CN104094854A - In-vivo ornamental plant in closed transparent container, and manufacturing method thereof - Google Patents
In-vivo ornamental plant in closed transparent container, and manufacturing method thereof Download PDFInfo
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- CN104094854A CN104094854A CN201410368461.4A CN201410368461A CN104094854A CN 104094854 A CN104094854 A CN 104094854A CN 201410368461 A CN201410368461 A CN 201410368461A CN 104094854 A CN104094854 A CN 104094854A
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Abstract
The invention provides an in-vivo ornamental plant in a closed transparent container, and a manufacturing method thereof. The manufacturing method comprises the following steps: selecting an explant, performing sterilization treatment on the explant, preparing a medium, inoculating the explant to the medium of inducing cluster buds, placing the medium to a culture chamber with appropriate light, temperature, and humidity for culture, performing continuous culture after cluster buds grow out, putting the explant with cluster buds to a medium suitable for proliferation, culturing at an appropriate environment, preparing an ornamental medium, selecting aseptic seedlings which have appropriate sizes and height, and grow well to be inoculated into the appropriate ornamental medium under an aseptic operation environment, culturing about two weeks in a culture chamber with appropriate light, temperature, and humidity, and then continuously culturing under a scattered light condition at the room temperature. According to the invention, a tissue culture rapid propagation technology is adopted, so that the ornamental form of the plant can be more novel, and the ornamental plant can be more beautiful, is sterile and sanitary, and quick in finished product growth speed as an explant form is adopted, can grow without watering, and is portable.
Description
Technical field
The invention belongs to novel household ornaments field, relate in particular to a kind of live body ornamental plant in sealing transparent vessel and preparation method thereof.
Background technology
In actual life, in order to experience the pure and fresh breath of the Nature whenever and wherever possible in the time in leisure, in the busy date, obtain loosening of the most comfortable, people conventionally stay at home or green plants is put by office, but green plants is difficult to arrange, time-to-live is not long, on market, has occurred now a kind ofly utilizing the mini flowers of making in plant tissue culture technique and gnotobasis as household decorations, meets the demand of people to flowers product.Plant tissue culture technique started from for 20 beginnings of the century, had formed a set of comparatively complete technical system and had progressively moved towards popular through Long-Term Scientific Study and engineering practice development.By sterile working, the organ of plant corpus, cell, organize even protoplast, be inoculated on the medium of artificial preparation, under manually operated environmental condition, cultivate, make it growth, breed or grow technology and the method for whole plant.Tissue culture technique has overcome the insurmountable difficulty of some routine techniques, has realized detoxification, regeneration, the Fast-propagation of plant, and is applied to crop, seedling production, has improved the reproductive efficiency of seedling.The mankind that develop into that are based upon the biotechnology on laboratory research basis have brought huge interests and wealth, and along with scientific and technological development, the intension of biotechnology also constantly changes.Biotechnology in plant most widely used general, obtain benefit maximum aspect be exactly fast breeding technique, it has broken through the space-time limitation that seedling is produced.
In prior art, open file (patent No. is the method for 200610007511.1 aseptic culture living plant artworks), its process be select as handicraft can sterilizing container and in natural environment growing plants seedling, plant is rinsed and asepticize processing repeatedly, prepare again medium, pour into container closure sterilizing, again by aseptic sprouting access container, use can filtered air ventilative cork sealing, the seedling survival rate of cultivating by said method is not high, survival rate is less than 40%, kind is few, one bottle of one flower, and the color of medium is single, moulding and kind are single.
Summary of the invention
Technical problem solved by the invention is to provide a kind of live body ornamental plant in sealing transparent vessel and preparation method thereof, thereby solves the problem in above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions:
A kind of live body ornamental plant in sealing transparent vessel, comprise sealing transparent vessel, medium, plants and accessories, described sealing transparent vessel is glass, the closed container of the difformity size of plastics or other transparent materials, described medium is taking MS medium as minimal medium, add different plant hormones according to different plants and be mixed with the medium of induced bundle bud and the medium of propagation, add pigment to be deployed into the medium of viewing and admiring of different colours, described plants is that the aseptic fast traditional font of setting up plant in sealing transparent vessel is, by explant material is carried out to sterilization treatment, after medium culture that is inoculated into induced bundle bud etc. grows clump bud, access in the medium of suitable propagation and continue to cultivate, be linked into again and view and admire just can growing and live body ornamental plant easy to carry without watering of turning out in medium in adapt circumstance, described accessories are designed to household pendulum decorations or mobile phone, ornament in knapsack and car.
A preparation method for live body ornamental plant in sealing transparent vessel, specifically comprises the steps:
(1) choosing of explant: choose Growth of Ornamental Plants stalwartness, without the suitable position of damage by disease and insect as explant, comprise embryo, endosperm, ovule, ovary, stem section, stem apex, stem tuber, bulb, blade, inflorescence, petal, flower pesticide, holder, fruit, seed, cell and protoplast;
(2) explant is carried out to sterilization treatment: tentatively clean with running water or detergent, then under the environment of sterile working, use disinfectant sterilizing, then use aseptic water washing, drain moisture and blot for subsequent use with filter paper;
(3) preparation of medium: first prepare MS medium, taking MS medium as minimal medium, add different plant hormones according to different plants and be mixed with the medium of induced bundle bud and the medium of propagation, packing after the pH of adjustment medium, the medium preparing is placed in to sterilizing 20min at 121 DEG C, standing, cooling for subsequent use, set up the sterile propagation system of plant;
(4) use aseptic utensil suitably to cut explant material, be inoculated on the medium of induced bundle bud, the culturing room that is placed in suitable illumination, temperature, humidity cultivates, and waits to grow and continues cultivation after clump bud, access on the medium of suitable propagation, in adapt circumstance, cultivate;
(5) view and admire the preparation of medium: taking MS medium as minimal medium, prepare with distilled water, add pigment to be deployed into the medium of viewing and admiring of different colours, add after the pH of medium is viewed and admired in different plant hormones and adjustment and be distributed in sealing transparent vessel according to different plants, the medium of viewing and admiring preparing is placed in to sterilizing 20min at 121 DEG C, standing, cooling for subsequent use;
(6) under the environment of sterile working, select suitable size, highly, well-grown aseptic seedling is inoculated into suitable viewing and admiring on medium, culturing room in suitable illumination, temperature, humidity cultivates about two weeks, proceeds to afterwards under room temperature, scattered light condition and continues to cultivate.
The preparation of described MS medium is first macroelement to be mixed with to 20 times of mother liquors, again trace element, organic principle, molysite are mixed with to 100 times of mother liquors, by adding agar 7.0g, sucrose 30g, macroelement mother liquor 50ml in every liter of MS medium, trace element mother liquor 10ml, organic principle mother liquor 10ml, mother liquid of iron salt 10ml, prepares and get final product with distilled water;
Described macroelement comprises NH
4nO
3, KNO
3, CaCl
22H
2o, MgSO
47H
2o, KH
2pO
4; By the weight ratio of each composition in every five liters of mother liquors be:
NH
4NO
3 165.0 g
KNO
3 190.0 g
CaCl
2·2H
2O 44.0 g
MgSO
4·7H
2O 37.0 g
KH
2PO
4 17.0 g；
Described trace element comprises KI, H
3bO
3, MnSO
44H
2o, ZnSO
47H
2o, Na
2moO
42H
2o, CuSO
45H
2o; The weight ratio that rises each composition in mother liquor by each is:
KI 83.0mg
H
3BO
3 620.0 mg
MnSO
4·4H
2O 2230.0 mg
ZnSO
4·7H
2O 860.0 mg
Na
2MoO
4·2H
2O 25.0 mg
CuSO
4·5H
2O 2.50 mg
CoCl
2·6H
2O 2.50 mg；
Described molysite comprises FeSO
47H
2o, Na
2eDTA2H
2o; By the weight ratio of each composition in a premium on currency be:
FeSO
4·7H
2O 2780.0 mg
Na
2EDTA·2H
2O 3730.0mg；
Described organic principle comprises inositol, nicotinic acid, puridoxine hydrochloride, thiamine hydrochloride, glycine; The weight ratio that rises each composition in mother liquor by each is:
Inositol 10000 mg
Nicotinic acid 50 mg
Puridoxine hydrochloride 50 mg
Thiamine hydrochloride 10 mg
Glycine 200mg.
The present invention has following beneficial effect:
A kind of live body ornamental plant in sealing transparent vessel of the present invention, maintenance is simple, pollen can not shed, owing to there being the bottle cap of breathing freely and preventing bacterium, the in-built medium of sealing transparent vessel, can supply with plant growth desired nutritional, therefore plant only need get final product normal growth at suitable illumination and temperature, removes the puzzlement of watering, fertilizing from.After plant root growth prosperity, after the laundering period, can be by plantlet of transplant in soil, plant grows normal development.The present invention utilizes tissue culture technology, plants ' aesthetics form is become to novel and beautiful more, Sterilized sanitation, has external packing sealing transparent vessel, various plants can be combined, play miniature potted landscape flower arrangement function, and can not damage by pressure, be convenient to transport, outer planting build, finished product is fast, just can grow and easy to carry without watering.
Brief description of the drawings
Fig. 1 is the structural representation that adopts the finger rose of cultivating in the vial with bottle cap of the inventive method making.
Shown in Fig. 1: 1, the vial with bottle cap; 2, medium; 3, finger rose; 4, ornament.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with specific embodiment, further set forth the present invention.
Referring to Fig. 1, the finger rose of cultivating in a kind of vial with bottle cap, comprises vial 1, medium 2, finger rose 3 and ornament 4 with bottle cap.
A preparation method of cultivating finger rose in the vial with bottle cap, specifically comprises the steps:
(1) choosing of explant: choose finger rose robust growth, without the stem section of damage by disease and insect as explant;
(2) explant is carried out to sterilization treatment: tentatively clean with running water or detergent, then under the environment of sterile working, use disinfectant sterilizing, then use aseptic water washing, drain moisture and blot for subsequent use with filter paper;
(3) preparation of medium: first prepare MS medium;
By macroelement by the weight ratio of each composition in five liters of mother liquors be first: NH
4nO
3165.0 g, KNO
3190.0 g, CaCl
22H
2o44.0 g, MgSO
47H
2o37.0 g, KH
2pO
417.0 g are mixed with 20 times of mother liquors;
By trace element by the weight ratio of each composition in one liter of mother liquor be again: KI83.0 mg, H
3bO
3620.0 mg, MnSO
44H
2o2230.0 mg, ZnSO
47H
2o860.0 mg, Na
2moO
42H
2o25.0 mg, CuSO
45H
2o2.50 mg; CoCl
26H
2o2.50 mg is mixed with 100 times of mother liquors;
By molysite by the weight ratio of each composition in one liter of mother liquor be again: FeSO
47H
2o2780.0 mg, Na
2eDTA2H
2o3730.0mg is mixed with 100 times of mother liquors;
By organic principle by the weight ratio of each composition in one liter of mother liquor be again: inositol 10000 mg, nicotinic acid 50 mg, puridoxine hydrochloride 50 mg, thiamine hydrochloride 10 mg, glycine 200mg are mixed with 100 times of mother liquors, by adding agar 7.0g, sucrose 30g, macroelement mother liquor 50ml in every liter of MS medium, trace element mother liquor 10ml, organic principle mother liquor 10ml, mother liquid of iron salt 10ml, prepares and get final product with distilled water;
Again taking MS medium as minimal medium, add plant hormone and be mixed with the medium of induced bundle bud and the medium of propagation, packing after the pH of adjustment medium, is placed in sterilizing 20min at 121 DEG C by the medium preparing, standing, cooling for subsequent use, set up the sterile propagation system of plant;
(4) use aseptic utensil suitably to cut explant material, be inoculated on the medium of induced bundle bud, the culturing room that is placed in sunny, temperature and is 18 DEG C-25 DEG C cultivates, and waits after growing clump bud and continues cultivation, access on the medium of suitable propagation, in adapt circumstance, cultivate;
(5) view and admire the preparation of medium: taking MS medium as minimal medium, add pigment to be deployed into the medium of viewing and admiring of different colours, add after the pH of medium is viewed and admired in different plant hormones and adjustment and be distributed in the vial with bottle cap according to different plants, the medium of viewing and admiring preparing is placed in to sterilizing 20min at 121 DEG C, standing, cooling for subsequent use;
(6) under the environment of sterile working, select suitable size, highly, well-grown aseptic seedling is inoculated into suitable viewing and admiring on medium, culturing room in suitable illumination, temperature, humidity cultivates about two weeks, proceed to afterwards under room temperature, scattered light condition and continue to cultivate, this product avoids being placed in hot environment, product is frangible, need be placed in the maintenance of smooth safe place, product should not firmly rock.
Also product can be transplanted, the growth of still can normally blooming, transplant first day bottle cap is opened to 1/3rd, within second day, open 2/3rds, allow plant slowly adapt to new environment, product is taken out gently in bottle, be placed in flowerpot, medium can be used for the sand or the earth that stir with fertilizer in basin, entangle plant with plastic paper, plant is placed in to the suitable place of sunny temperature, keep ground moistening, unclamp film paper some every day, film paper can be removed until plant shakes down completely.
More than show and described general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (3)
1. the live body ornamental plant in sealing transparent vessel, it is characterized in that: comprise sealing transparent vessel, medium, plants and accessories, described sealing transparent vessel is band base test tube or vial with cover or band plug circular glass cylinder, described medium is taking MS medium as minimal medium, add different plant hormones according to different plants and be mixed with the medium of induced bundle bud and the medium of propagation, add pigment to be deployed into the medium of viewing and admiring of different colours, described plants is that the aseptic fast traditional font of setting up plant in sealing transparent vessel is, by explant material is carried out to sterilization treatment, after medium culture that is inoculated into induced bundle bud etc. grows clump bud, access in the medium of suitable propagation and continue to cultivate, be linked into again and view and admire just can growing and live body ornamental plant easy to carry without watering of turning out in medium in adapt circumstance, described accessories are designed to household pendulum decorations or mobile phone, ornament in knapsack and car.
2. a preparation method for the live body ornamental plant in sealing transparent vessel, is characterized in that: specifically comprise the steps:
(1) choosing of explant: choose Growth of Ornamental Plants stalwartness, without the suitable position of damage by disease and insect as explant, comprise embryo, endosperm, ovule, ovary, stem section, stem apex, stem tuber, bulb, blade, inflorescence, petal, flower pesticide, holder, fruit, seed, cell and protoplast;
(2) explant is carried out to sterilization treatment: tentatively clean with running water or detergent, then under the environment of sterile working, use disinfectant sterilizing, then use aseptic water washing, drain moisture and blot for subsequent use with filter paper;
(3) preparation of medium: first prepare MS medium, taking MS medium as minimal medium, add different plant hormones according to different plants and be mixed with the medium of induced bundle bud and the medium of propagation, packing after the pH of adjustment medium, the medium preparing is placed in to sterilizing 20min at 121 DEG C, standing, cooling for subsequent use, set up the sterile propagation system of plant;
(4) use aseptic utensil suitably to cut explant material, be inoculated on the medium of induced bundle bud, the culturing room that is placed in suitable illumination, temperature, humidity cultivates, and waits to grow and continues cultivation after clump bud, access on the medium of suitable propagation, in adapt circumstance, cultivate;
(5) view and admire the preparation of medium: taking MS medium as minimal medium, prepare with distilled water, add pigment to be deployed into the medium of viewing and admiring of different colours, add after the pH of medium is viewed and admired in different plant hormones and adjustment and be distributed in sealing transparent vessel according to different plants, the medium of viewing and admiring preparing is placed in to sterilizing 20min at 121 DEG C, standing, cooling for subsequent use;
(6) under the environment of sterile working, select suitable size, highly, well-grown aseptic seedling is inoculated into suitable viewing and admiring on medium, culturing room in suitable illumination, temperature, humidity cultivates about two weeks, proceeds to afterwards under room temperature, scattered light condition and continues to cultivate.
3. the preparation method of a kind of live body ornamental plant in sealing transparent vessel according to claim 2, it is characterized in that: the preparation of described MS medium is first macroelement to be mixed with to 20 times of mother liquors, again trace element, organic principle, molysite are mixed with to 100 times of mother liquors, by adding agar 7.0g, sucrose 30g, macroelement mother liquor 50ml in every liter of MS medium, trace element mother liquor 10ml, organic principle mother liquor 10ml, mother liquid of iron salt 10ml, prepares and get final product with distilled water;
Described macroelement comprises NH
4nO
3, KNO
3, CaCl
22H
2o, MgSO
47H
2o, KH
2pO
4; By the weight ratio of each composition in every five liters of mother liquors be:
NH
4NO
3 165.0 g
KNO
3 190.0 g
CaCl
2·2H
2O 44.0 g
MgSO
4·7H
2O 37.0 g
KH
2PO
4 17.0 g；
Described trace element comprises KI, H
3bO
3, MnSO
44H
2o, ZnSO
47H
2o, Na
2moO
42H
2o, CuSO
45H
2o; The weight ratio that rises each composition in mother liquor by each is:
KI 83.0mg
H
3BO
3 620.0 mg
MnSO
4·4H
2O 2230.0 mg
ZnSO
4·7H
2O 860.0 mg
Na
2MoO
4·2H
2O 25.0 mg
CuSO
4·5H
2O 2.50 mg
CoCl
2·6H
2O 2.50 mg；
Described molysite comprises FeSO
47H
2o, Na
2eDTA2H
2o; By the weight ratio of each composition in a premium on currency be:
FeSO
4·7H
2O 2780.0 mg
Na
2EDTA·2H
2O 3730.0mg；
Described organic principle comprises inositol, nicotinic acid, puridoxine hydrochloride, thiamine hydrochloride, glycine; The weight ratio that rises each composition in mother liquor by each is:
Inositol 10000 mg
Nicotinic acid 50 mg
Puridoxine hydrochloride 50 mg
Thiamine hydrochloride 10 mg
Glycine 200mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410368461.4A CN104094854A (en) | 2014-07-30 | 2014-07-30 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410368461.4A CN104094854A (en) | 2014-07-30 | 2014-07-30 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104094854A true CN104094854A (en) | 2014-10-15 |
Family
ID=51663691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410368461.4A Pending CN104094854A (en) | 2014-07-30 | 2014-07-30 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900844A (en) * | 2016-05-13 | 2016-08-31 | 大连民族大学 | Microscopic ornamental plant and culture method thereof |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415335A (en) * | 2011-08-31 | 2012-04-18 | 华中农业大学 | Manufacture method of angiopteris esculenta tube flowers |
| CN202588020U (en) * | 2012-05-15 | 2012-12-12 | 山东农业大学 | Ornamental miniature foliage plant product |
| CN103385166A (en) * | 2012-05-11 | 2013-11-13 | 河北农业大学 | Method for cultivating pocket ornamental Ludi king by tissue culture technology |
-
2014
- 2014-07-30 CN CN201410368461.4A patent/CN104094854A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415335A (en) * | 2011-08-31 | 2012-04-18 | 华中农业大学 | Manufacture method of angiopteris esculenta tube flowers |
| CN103385166A (en) * | 2012-05-11 | 2013-11-13 | 河北农业大学 | Method for cultivating pocket ornamental Ludi king by tissue culture technology |
| CN202588020U (en) * | 2012-05-15 | 2012-12-12 | 山东农业大学 | Ornamental miniature foliage plant product |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900844A (en) * | 2016-05-13 | 2016-08-31 | 大连民族大学 | Microscopic ornamental plant and culture method thereof |
| CN105900844B (en) * | 2016-05-13 | 2018-07-31 | 大连民族大学 | A kind of miniature ornamental plant and its cultural method |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| CN111642380A (en) * | 2019-12-03 | 2020-09-11 | 南阳师范学院 | Process flow of micro landscape artwork in test tube |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
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Application publication date: 20141015 |
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