CN103385166A - Cultivation method of pocket ornamental philodendron cv. wendimbe by utilization of tissue culture technology - Google Patents
Cultivation method of pocket ornamental philodendron cv. wendimbe by utilization of tissue culture technology Download PDFInfo
- Publication number
- CN103385166A CN103385166A CN2012101442753A CN201210144275A CN103385166A CN 103385166 A CN103385166 A CN 103385166A CN 2012101442753 A CN2012101442753 A CN 2012101442753A CN 201210144275 A CN201210144275 A CN 201210144275A CN 103385166 A CN103385166 A CN 103385166A
- Authority
- CN
- China
- Prior art keywords
- tissue culture
- bottle
- ornamental
- wendimbe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a cultivation method of pocket ornamental philodendron cv. wendimbe by utilization of tissue culture technology. Philodendron cv. wendimbe tissue culture seedling, through test screening, can adapt to a bottle mouth closed condition, is not strict in requirements of temperature and light, has a long survival period in indoor natural conditions, has good ornamental value and is a suitable pocket ornamental flower breed for cultivation. During the preparation of the pocket ornamental philodendron cv. wendimbe, beautifully designed vases and jars are selected and used as culture vessels, a culture medium is MS + BA 0.5mg/L + NAA 0.05mg/L + white granulated sugar 50.0g/L + agar 6.2g/L, and subpackage amount is accounted for 1/5-1/4 of the volume of a bottle; about 5% of grape purple, carmine, lemon yellow and other food colourants can be added into the colorless transparent agar culture medium for increasing ornamental effect; after inoculation of subculture sprout clumpy buds or young shoots of the philodendron cv. wendimbe, a bottle mouth is sealed, and then the bottle is decorated with colorized packing paper. The philodendron cv. wendimbe tissue culture seedling prepared by the technology is simple in method, can be placed for 14 months on a bedside, a table side and a dining table in indoors; plantlets in the bottle can gradually differentiate and grow until the whole bottle is fully filled, and are full of life and very beautiful.
Description
Technical field
The present invention relates to a kind of tissue culture technique that utilizes and cultivate pocket method of viewing and admiring green emperor.
Background technology
It is comparatively popular on Korea S, market, Taiwan in recent years that mini to carry flowers be the test tube flowers, be referred to as again " angel's greenhouse ", be utilize tissue culture technique with plant seedling growth in the large cuvette of thumb or art up vial, due to plant and container small, being particularly suitable for making becomes various ornaments, as necklace, mobile phone chain etc.
The pocket ornamental plants that the present invention cultivates is mainly used in interior decoration.Society, raising due to living standard, people more and more pay attention to the beautifying and decorating of room, working environment, the ornamental plants of some small and exquisite top grades can allow the people in busy city life, enjoy at any time the green that the Nature is given, experience life and life, day by day be subject to consumer's favor.Along with development and the extensive use of plant tissue culture technique, utilize tissue culture technology to develop pocket gift flower grass in bottle, be about to ornamental plants and cultivate in aseptic medium, be equipped with special-shape bottle and unique decoration of beautiful design, make it have more sight.These pocket flowers bottlenecks sealings, sanitation and hygiene, small and exquisite, can be placed on the head of a bed, case limit, dining table, removed the loaded down with trivial details of common flowers daily management from, can meet grow flower love flower people's the demand of knowledge of those shortages, adapt to the allegro life style of modern.The pocket flowers of group training are unique novel, can be used as a kind of art work of elegance, have wide market prospects.
Cultivate pocket flowers and be different from the conventional organization culture technique, general group training seedling just needed subculture once about two months, the medium that more renews, and as pocket flowers in the bottle of gift need in the situation that not subculture cultivate and to survive considerable time, so blake bottle is airtight, keep the moisture of medium, also will delay the growth of test-tube plantlet, to reduce nutrient consumption; Its growing environment more complicated, can not have superior temperature, illumination condition as conventional group training chamber simultaneously; And in bottle, plant can also normal growth, does not reduce ornamental value, and this just needs to adopt suitable ornamental plants kind and culture technique.
Summary of the invention
The object of the invention is to, set up a kind of method of utilizing tissue culture technique to cultivate pocket ornamental plants in bottle.
The technical solution adopted for the present invention to solve the technical problems is: at first utilize tissue culture technique to filter out the ornamental plants kind that can grow in Indoor Natural condition, closed environment, and regulate and control its growth, cultivate the group training seedling that can survive for a long time; Ornamental plants group training seedling is cultivated in the aseptic bottle of beautiful design, and in addition unique decoration, give different implied meanings, makes it have more artistic appreciation.
Particular content of the present invention, i.e. the concrete grammar of the pocket ornamental plants of cultivation group training is as follows:
1. screen suitable ornamental plants kind
Green emperor likes the green emperor of woods taro another name, emperor, evergreen wooden Climbing Plant, and its profile is graceful, and refined gas is pure, is the good ornamental foliage plant of arranging household, office space, and is very popular.Through experiment sieving, green emperor organizes the training seedling can adapt to airtight, the ventilative bad condition of bottleneck; Temperature, illumination are required not strict, well-grown under 14 ~ 33 ℃ of temperature ranges, Indoor Natural scattered light; In bottle, plant type is short and small, and nutrient consumption is few, and survival period is long, has higher ornamental value, is suitable pocket ornamental flower kind.
2. green emperor organizes the foundation of training system
Gather the potted plant seedling terminal bud of green emperor or axillary bud stem section, running water is rinsed well, sterilized 10 ~ 12 minutes with 0.1% mercuric chloride solution under aseptic condition, aseptic water washing 4 ~ 5 times, be inoculated on MS+BA1.0mg/L+NAA0.05mg/L+ white granulated sugar 30.0g/L+ agar 6.0g/L subculture medium, regulate pH6.0 before medium sterilization; To breed cultivation less than the clump bud of pollution, normal differentiation, about 2 months, once, condition of culture is 25 ± 3 ℃ of temperature to subculture, intensity of illumination 2000lx, light application time 10-14h/d.
Pocket view and admire green emperor organize the training seedling making
(1) organize the growth of training seedling for delaying green emperor, extend its holding time, the medium that group training seedling is viewed and admired in preparation is adjusted into: MS+BA0.5mg/L+NAA0.05mg/L+ white granulated sugar 50.0g/L+ agar 6.0g/L, the pH6.0 left and right,, for increasing appreciation effect, can add approximately 5% the food colorings such as greyish purple, carmine, lemon yellow in water white agar medium.
(2) select vase, the Cans of beautiful design, the medium that packing accounts for bottle long-pending 1/5 ~ 1/4,, if in bottle, space is larger, can hold more medium, and the holding time is longer.The green emperor's subculture seedling clump bud of inoculation or the tender tip after autoclaving, airtight bottleneck, then with color packing paper, decorate.
That (3) makes pocketly views and admires green emperor and organizes training seedling the longest placement 14 months on the indoor head of a bed, case limit, dining table, and in bottle, a seedling breaks up gradually, grows until be full of whole bottle, and is full of life, very attractive in appearance.
The beneficial effect of patent of the present invention is, filtered out and can adapt to bottleneck airtight condition, common room working environment, and survival period is long, and suitable for making is viewed and admired the group training flower variety-green emperor of gift; Utilize that this fabrication techniques is pocket views and admires green emperor, method is simple, and appreciation effect is good, and the holding time is long, can be used as novelty, unique making a present, and has wide market prospects.
Description of drawings
Fig. 1 pocketly views and admires green emperor and organizes the training seedling
Embodiment
Between 2006-2007, on MS+BA1.0mg/L+NAA0.05mg/L+ white granulated sugar 30.0g/L+ agar 6.0g/L proliferated culture medium, the ornamental foliage plant group training seedlings such as inoculation emerald, green emperor, leopard's spots arrowroot, cat's eye arrowroot, seven color tis and codiaeum variegatum, adopt respectively tampon to add sealed membrane gas permeable material and vinyon sealing triangular flask bottleneck, be put in the indoor cultivation of common sunny side and the back (duration of test room temperature at 14 ℃ ~ 33 ℃, natural scattering light) after inoculation.Result investigation shows, all kind groups training seedlings, adopt the breathable sealing material, equal well-growns, but medium evaporates gradually, exhausts, and the In Vitro conservation time is in 80 ~ 120d left and right.The group training seedling growth rate of airtight bottleneck slows down, and medium moisture is difficult for evaporation, and the holding time extends, but leopard's spots arrowroot and cat's eye arrowroot have the glass seedling to occur, and yellow leaf appears in seven color tis and codiaeum variegatum, and the holding time can reach 180d; The emerald greenery are healthy and strong, just have aerial root to occur, it is in disorder that plant type shows slightly, and can preserve 240d; That upgrowth situation is best is green emperor, and green bud is grown thickly always, until medium is fully dry during 380d.Filter out thus green emperor and make the group training seedling kind of pocket ornamental flower for optimum.
2008-2009, organize the growth of training seedling for delaying green emperor, extend its holding time, further test is adjusted into MS+BA0.5mg/L+NAA0.05mg/L+ white granulated sugar 50.0g/L+ agar 6.0g/L with medium, the pH6.0 left and right, and add some food coloring pigment, make colored medium, to strengthen appreciation effect.Medium is distributed in the vase, Cans of beautiful design, dispensed loading amount slightly Duo and airtight bottleneck during than Sterile culture, to extend the holding time, and the green emperor's subculture seedling clump bud of inoculation or the tender tip after autoclaving, with color packing paper wrapping decoration.What method was made thus pocketly views and admires green emperor and organizes the training seedling after indoor placement 420d, and medium is fully dry, and in this moment bottle, seedling differentiation, growth have been full of whole bottle, and be full of life, very attractive in appearance.
Claims (6)
1. one kind is utilized tissue culture technology to cultivate pocket method of viewing and admiring green emperor, it is characterized in that utilizing tissue culture technique to filter out the ornamental plants kind that can grow in Indoor Natural condition, closed environment, and regulate and control its growth, cultivate the group training seedling that can survive for a long time; Ornamental plants group training seedling is cultivated in the aseptic bottle of beautiful design, and in addition unique decoration, make it have artistic appreciation.
2. the tissue culture technology that utilizes according to claim 1 is cultivated pocket method of viewing and admiring green emperor, it is characterized in that green emperor organizes the training seedling and can adapt to the bottleneck airtight condition, require not strict to temperature, illumination, indoor survival period is long, have higher ornamental value, be the pocket ornamental flower kind of suitable cultivation.
3. the tissue culture technology that utilizes according to claim 1 is cultivated pocket method of viewing and admiring green emperor, it is characterized in that culture vessel selects the vase of beautiful design, Cans etc.
4. the tissue culture technology that utilizes according to claim 1 is cultivated pocket method of viewing and admiring green emperor, it is characterized in that for extend pocket view and admire green emperor organize the training seedling time-to-live, medium is MS+BA0.5mg/L+NAA0.05mg/L+ white granulated sugar 50.0g/L+ agar 6.2g/L, the pH6.0 left and right, it is many that the medium dispensed loading amount is wanted, account for bottle long-pending 1/5 ~ 1/4.
5. the tissue culture technology that utilizes according to claim 1 is cultivated pocket method of viewing and admiring green emperor, it is characterized in that for increasing appreciation effect, can add approximately 5% the food colorings such as greyish purple, carmine, lemon yellow in water white agar medium.
6. the tissue culture technology that utilizes according to claim 1 is cultivated pocket method of viewing and admiring green emperor, after it is characterized in that inoculating green emperor's subculture seedling clump bud or the tender tip, and airtight bottleneck, then with color packing paper, decorate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210144275.3A CN103385166B (en) | 2012-05-11 | 2012-05-11 | A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210144275.3A CN103385166B (en) | 2012-05-11 | 2012-05-11 | A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103385166A true CN103385166A (en) | 2013-11-13 |
| CN103385166B CN103385166B (en) | 2016-08-10 |
Family
ID=49529730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210144275.3A Expired - Fee Related CN103385166B (en) | 2012-05-11 | 2012-05-11 | A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103385166B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN105900844A (en) * | 2016-05-13 | 2016-08-31 | 大连民族大学 | Microscopic ornamental plant and culture method thereof |
| CN106016490A (en) * | 2016-07-13 | 2016-10-12 | 大连民族大学 | Ecotype air purifier |
| CN106386510A (en) * | 2016-12-06 | 2017-02-15 | 肇庆学院 | Manufacturing method of Anoectochilus roxburghii test tube flowers |
| WO2019119653A1 (en) * | 2017-12-22 | 2019-06-27 | 绵阳师范学院 | Culture medium for tissue culture of fagus longipetiolata plants and culture method therefor |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU617096B2 (en) * | 1987-01-19 | 1991-11-21 | Ashley Corporation Pty. Ltd | A philodendron cultivar |
| CN2222077Y (en) * | 1995-05-02 | 1996-03-13 | 武汉市林业果树科学研究所 | Soilless growing plant art and craft potted landscape |
| CN201484047U (en) * | 2009-09-05 | 2010-05-26 | 浙江省农业科学院 | Miniature tissue-culture living-plant pendant ornament |
-
2012
- 2012-05-11 CN CN201210144275.3A patent/CN103385166B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU617096B2 (en) * | 1987-01-19 | 1991-11-21 | Ashley Corporation Pty. Ltd | A philodendron cultivar |
| CN2222077Y (en) * | 1995-05-02 | 1996-03-13 | 武汉市林业果树科学研究所 | Soilless growing plant art and craft potted landscape |
| CN201484047U (en) * | 2009-09-05 | 2010-05-26 | 浙江省农业科学院 | Miniature tissue-culture living-plant pendant ornament |
Non-Patent Citations (4)
| Title |
|---|
| M. A. K. AZAD ET AL.: "PLANT REGENERATION FROM MESOPHYLL PROTOPLASTS OF A MEDICINAL PLANT，PHELLODENDRON AMURENSE RUPR.", 《IN VITRO CELL. DEV. BIOL.—PLANT》, vol. 42, 31 December 2006 (2006-12-31), pages 502 - 507 * |
| 周长东: "绿帝王组织培养快速繁殖技术", 《山西林业科技》, no. 1, 31 March 2006 (2006-03-31) * |
| 朱根发: "蔓绿绒属观赏植物的组织培养快速繁殖技术", 《植物学通报》, vol. 20, no. 3, 31 December 2003 (2003-12-31), pages 342 - 345 * |
| 朱西儒等主编: "《商品花卉生产以及保鲜技术》", 30 June 2001, article "绿帝王喜林芋" * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858760A (en) * | 2014-02-26 | 2014-06-18 | 福建省农业科学院作物研究所 | Manufacturing method of test-tube type anoectohilus formosanus |
| CN103858760B (en) * | 2014-02-26 | 2016-04-13 | 福建省农业科学院作物研究所 | The preparation method of a kind of roxburgh anoectochilus terminal bud test tube flowers |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN105900844A (en) * | 2016-05-13 | 2016-08-31 | 大连民族大学 | Microscopic ornamental plant and culture method thereof |
| CN106016490A (en) * | 2016-07-13 | 2016-10-12 | 大连民族大学 | Ecotype air purifier |
| CN106386510A (en) * | 2016-12-06 | 2017-02-15 | 肇庆学院 | Manufacturing method of Anoectochilus roxburghii test tube flowers |
| WO2019119653A1 (en) * | 2017-12-22 | 2019-06-27 | 绵阳师范学院 | Culture medium for tissue culture of fagus longipetiolata plants and culture method therefor |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103385166B (en) | 2016-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103385166B (en) | A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor | |
| CN107006353A (en) | A kind of rose cuttage breeding method and a kind of rose indoor cultivation method for Ganoderma | |
| CN102217549B (en) | Culture method of rieger begonias test tube flowers | |
| CN103069989A (en) | Method for producing esthetic bryophyte bottle | |
| CN107018905B (en) | A kind of bottle orchid test tube seedling preserving seed method | |
| CN202588020U (en) | Ornamental miniature foliage plant product | |
| CN105532398B (en) | A kind of hosta tissue culture seedling Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method | |
| CN106171996B (en) | A kind of rapid propagation method of wild white birch | |
| CN105028213A (en) | Tissue-culturing rapid propagation method for dendrobium officinale | |
| CN104094854A (en) | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof | |
| CN107027461A (en) | The method of pinching quickly breeding Growth anddevelopment potted flower | |
| CN107683768B (en) | A kind of acclimatization and transplants method of the syringa reticulata var mandshurica tissue-cultured seedling of callus induction | |
| CN100391333C (en) | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum | |
| CN108848977A (en) | A kind of promotion blueberry tissue-cultured seedling rooting method | |
| CN201153429Y (en) | Flower-pot for cultivation without soil | |
| CN112042536A (en) | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape | |
| CN104255488B (en) | The cultural method of a kind of single-lobe petunia test tube flowers | |
| CN1132769C (en) | Closed composite plant enjoying container | |
| CN104541860A (en) | Manufacturing process for lightweight pitaya pot culture | |
| CN104365477A (en) | Secondary proliferation culture method of color osmanthusfragrans test-tube plantlets | |
| CN110419374A (en) | A kind of breeding method of the tree-like cultivation mode of colorful fruit variety | |
| CN104542271A (en) | Mustard soilless cultivation method | |
| CN107691159A (en) | A kind of potted plant sweet potato cultivation method | |
| CN2471102Y (en) | Cultivated pot for plant and flower | |
| CN201659840U (en) | Tissue culture living plant ornament |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 Termination date: 20170511 |