CN103385166B - A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor - Google Patents
A kind of utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor Download PDFInfo
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- CN103385166B CN103385166B CN201210144275.3A CN201210144275A CN103385166B CN 103385166 B CN103385166 B CN 103385166B CN 201210144275 A CN201210144275 A CN 201210144275A CN 103385166 B CN103385166 B CN 103385166B
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Abstract
The invention discloses a kind of method utilizing tissue culture technology to cultivate pocket ornamental plant, through experiment sieving, green emperor's tissue cultured seedling adapts to bottleneck airtight condition, the strictest to temperature, light as requested, under the conditions of Indoor Natural, survival period is long, and sight is good, for suitably cultivating pocket ornamental flower kind.Prepare pocket when viewing and admiring green emperor culture vessel select the vase of beautiful design, Cans；Culture medium is MS+BA0.5mg/L+NAA0.05mg/L+ white sugar 50.0g/L+ agar 6.2g/L, and dispensed loading amount accounts for 1/5 ~ 1/4 that bottle is long-pending；For increasing appreciation effect, the food coloring such as greyish purple, carmine, lemon yellow of about 5% can be added in water white agar culture medium；After inoculating green emperor's Regenerated plant clump bud or the tender tip, airtight bottleneck, then decorate with color packing paper.Utilizing this technology to make and pocket view and admire green emperor's tissue cultured seedling, method is simple, can place up to 14 months on the indoor head of a bed, case limit, dining table, and in bottle, seedling gradually breaks up, grow until full whole bottle, full of life, the most attractive in appearance.
Description
Technical field
The present invention relates to a kind of utilize tissue culture technique cultivate the pocket method viewing and admiring green emperor.
Background technology
The most popular on Korea S, market, Taiwan in recent years mini carry flowers i.e. test tube flowers, it is referred to as again " angel's greenhouse ", it is to utilize tissue culture technique by plant seedling growth in the big cuvette of thumb or art up vial, owing to plant and container are small, it is particularly suitable for making and becomes various ornament, such as necklace, mobile phone chain etc..
The pocket ornamental plant that the present invention cultivates is mainly used in interior decoration.Society, raising due to living standard, the beautifying and decorating in room, working environment is increasingly paid attention to by people, the ornamental plant of some small and exquisite top grades can allow people in busy city life, enjoy the green that the Nature gives at any time, experience life and life, be increasingly subject to consumers.Along with development and the extensively application of plant tissue culture technique, utilize tissue culture technology to develop pocket gift flower grass in bottle, will cultivate in aseptic culture medium by ornamental plant, be equipped with the special-shape bottle of beautiful design and the decoration of uniqueness so that it is more sight.These pocket flowers bottlenecks are closed, and sanitation and hygiene are small and exquisite, can be placed on the head of a bed, case limit, dining table, eliminate the loaded down with trivial details of common flowers daily management, can meet those love lacking knowledge of growing flower flower Man's Demands, adapt to the allegro life style of modern.It is novel that group trains pocket flowers uniqueness, can have wide market prospect as the art work of a kind of elegance.
Cultivate pocket flowers and be different from conventional organization culture technique, general tissue cultured seedling about two months just needs subculture once, the culture medium more renewed, and need to survive considerable time in the case of not successive transfer culture as pocket flowers in the bottle of gift, so culture bottle is airtight, keeping the moisture of culture medium, the growth of test tube Seedling to be delayed, to reduce nutrient consumption；Its growing environment is more complicated simultaneously, it is impossible to as there are superior temperature, illumination condition in routine group training room；And in bottle plant can also normal growth, do not reduce ornamental value, this is accomplished by using suitable ornamental plant kind and culture technique.
Summary of the invention
It is an object of the invention to, set up and a kind of utilize tissue culture technique to cultivate the method for pocket ornamental plant in bottle.
The technical solution adopted for the present invention to solve the technical problems is: filters out the ornamental plant kind that can grow in Indoor Natural condition, closed environment first with tissue culture technique, and regulates and controls its growth, cultivates the tissue cultured seedling that can survive for a long time；Ornamental plant tissue cultured seedling is cultivated in the aseptic bottle of beautiful design, in addition unique decoration, give different implied meaning so that it is more artistic appreciation.
The particular content of the present invention, i.e. the concrete grammar that cultivation group trains pocket ornamental plant is as follows:
1. screen suitable ornamental plant kind
Green emperor likes woods taro another name green emperor, emperor, evergreen wooden Climbing Plant, and its profile is graceful, and refined gas is pure, is to arrange household, the excellent foliage plant of office space, very popular.Through experiment sieving, green emperor's tissue cultured seedling adapts to bottleneck bad condition airtight, ventilative；Not strict to temperature, light as requested, well-grown under 14 ~ 33 DEG C of temperature ranges, Indoor Natural scattered light；In bottle, plant type is short and small, and nutrient consumption is few, and survival period is long, has higher ornamental value, for suitable pocket ornamental flower kind.
The greenest emperor organizes the foundation of nurture system
Gather green emperor's Potted orchard terminal bud or axillary bud stem section, tap water is rinsed well, sterilize 10 ~ 12 points with 0.1% mercuric chloride solution under aseptic condition, aseptic water washing 4 ~ 5 times, it is inoculated on MS+BA1.0mg/L+NAA0.05mg/L+ white sugar 30.0g/L+ agar 6.0g/L subculture medium, before medium sterilization, regulates pH6.0；Pollution, the clump bud of normal differentiation will not had to carry out enrichment culture, once, condition of culture is temperature 25 ± 3 DEG C to about 2 months subcultures, intensity of illumination 2000lx, light application time 10-14h/d.
The most pocket making viewing and admiring green emperor's tissue cultured seedling
(1) for delaying green emperor's tissue cultured seedling to grow, extend its holding time, preparation is viewed and admired the culture medium of tissue cultured seedling and is adjusted to: MS+BA0.5mg/L+NAA0.05mg/L+ white sugar 50.0g/L+ agar 6.0g/L, about pH6.0, for increasing appreciation effect, the food coloring such as greyish purple, carmine, lemon yellow of about 5% can be added in water white agar culture medium.
(2) selecting the vase of beautiful design, Cans, subpackage accounts for bottle and amasss the culture medium of 1/5 ~ 1/4, if space is relatively big in bottle, can accommodate more culture medium, and the holding time is longer.Green emperor's Regenerated plant clump bud or the tender tip is inoculated after autoclaving, airtight bottleneck, then with color packing paper decoration.
(3) that makes pocket views and admires that green emperor's tissue cultured seedling is the longest on the indoor head of a bed, case limit, dining table to be placed 14 months, and in bottle, seedling gradually breaks up, grow until full whole bottle, full of life, the most attractive in appearance.
Patent of the present invention provides the benefit that, has filtered out and has adapted to bottleneck airtight condition, common household working environment, and survival period is long, and suitable for making views and admires the group training green emperor of flower variety of gift；Utilizing that this fabrication techniques is pocket views and admires green emperor, method is simple, and appreciation effect is good, and the holding time is long, can make a present as novel, unique, have wide market prospect.
Accompanying drawing explanation
Fig. 1 is pocket to view and admire green emperor's tissue cultured seedling
Detailed description of the invention
Between 2006-2007, on MS+BA1.0mg/L+NAA0.05mg/L+ white sugar 30.0g/L+ agar 6.0g/L proliferated culture medium, inoculation emerald, green emperor, leopard's spots Rhizoma Marantae arundinaceae, cat's eye arrowroot, the seven color foliage plant tissue cultured seedlinies such as ti and Codiaeum Variegatum (L.) Bl., it is respectively adopted tampon and adds sealed membrane gas permeable material and vinyon closing triangular flask bottleneck, common sunny side and back indoor cultivation (experimental period room temperature at 14 DEG C ~ 33 DEG C, natural scattering light) it is put in after inoculation.Result investigation shows that all kind tissue cultured seedlinies use breathable sealing material, equal well-grown, but culture medium is gradually evaporated, exhausted, and the In Vitro conservation time is at about 80 ~ 120d.The tissue cultured seedling speed of growth of airtight bottleneck slows down, and culture medium moisture is difficult to evaporation, and the holding time extends, but leopard's spots Rhizoma Marantae arundinaceae and cat's eye arrowroot have vitreous shoot to occur, and yellow leaf occur in seven color tis and Codiaeum Variegatum (L.) Bl., and the holding time is up to 180d；Emerald greenery are healthy and strong, simply have aerial root to occur, plant type shows slightly in disorder, can preserve 240d；The greenest emperor of upgrowth situation, the greenest bud is grown thickly, until culture medium is the most dry during 380d.Thus filter out green emperor and make the tissue cultured seedling kind of pocket ornamental flower for optimum.
2008-2009, for delaying green emperor's tissue cultured seedling to grow, extend its holding time, culture medium is adjusted to MS+BA0.5mg/L+NAA0.05mg/L+ white sugar 50.0g/L+ agar 6.0g/L by test further, about pH6.0, and add some food coloring pigment, make colored culture medium, to strengthen appreciation effect.Culture medium being distributed in the vase of beautiful design, Cans, dispensed loading amount is more than during Sterile culture and airtight bottleneck, to extend the holding time, inoculates green emperor's Regenerated plant clump bud or the tender tip after autoclaving, with color packing paper wrapping decoration.What thus method was made pocket views and admires green emperor's tissue cultured seedling after indoor placement 420d, and culture medium is the most dry, and now in bottle, little seedling differentiation, growth are filled with whole bottle, full of life, the most attractive in appearance.
Claims (4)
1. one kind utilizes tissue culture technology to cultivate the pocket method viewing and admiring green emperor, it is characterised in that including:
Step 1, utilizes tissue culture technique to filter out the ornamental plant kind that can grow in Indoor Natural condition, closed environment；
Step 2, uses the ornamental plant breed of variety filtered out to go out the ornamental plant tissue cultured seedling of long-time survival, views and admires described
Plant tissue culture Seedling is sealed in aseptic bottle, and is decorated so that it is have artistic appreciation；
Described step 1 specifically includes:
Add BA1.0mg/L at MS to add NAA0.05mg/L and add on agar 6.0g/L proliferated culture medium with white sugar 30.0g/L, connect
Planting the tissue cultured seedling of multiple foliage plant, wherein, described foliage plant tissue cultured seedling includes green emperor, emerald, leopard's spots Rhizoma Marantae arundinaceae, cat
Eye Rhizoma Marantae arundinaceae, seven color ti and Codiaeum Variegatum (L.) Bl.；
The described tissue cultured seedling of inoculation is respectively placed in employing tampon add the sealing of sealed membrane gas permeable material and use the airtight envelope of vinyon
Indoor cultivation is carried out, it is thus achieved that the result of the test of each tissue cultured seedling indoor cultivation in the triangular flask of mouth；Wherein, condition of culture is: room temperature
At 14 DEG C～33 DEG C, natural scattering light；
According to described result of the test, screening according to each tissue cultured seedling growth conditions, determining that green emperor's tissue cultured seedling is for can adapt to bottle
Mouth airtight condition, strict to temperature, light as requested, indoor survival period is long, has the pocket ornamental flower of higher ornamental value
Kind；Wherein, described tissue cultured seedling growth conditions includes: the tissue cultured seedling speed of growth, culture medium moisture evaporation situation, tissue cultured seedling are protected
Deposit the time；
Described step 2 specifically includes:
Gathering green emperor's Potted orchard terminal bud or axillary bud stem section, tap water is rinsed well, disappears with 0.1% mercuric chloride solution under aseptic condition
Poison 10～12 minutes, aseptic water washing 4～5 times；
Pollution, the clump bud of normal differentiation will not had to carry out enrichment culture, once, condition of culture is temperature 25 ± 3 DEG C to 2 months subcultures,
Intensity of illumination 2000lx, light application time 10-14h/d；
MS adds BA0.5mg/L add the subculture medium that NAA0.05mg/L adds agar 6.0g/L with white sugar 50.0g/L and be loaded on
In described aseptic bottle, regulate pH6.0, after autoclaving, inoculate green emperor's Regenerated plant clump bud or stem section, airtight bottleneck, then with coloured silk
Color wrapping paper is decorated.
The most according to claim 1 utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor, it is characterised in that described
Aseptic bottle selects the vase or Cans that space is big in beautiful design, bottle.
The most according to claim 1 utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor, it is characterised in that in nothing
The agar culture medium that color is transparent adds the food coloring of about 5%.
The most according to claim 3 utilize tissue culture technology cultivate the pocket method viewing and admiring green emperor, it is characterised in that described
Food coloring includes greyish purple, carmine, lemon yellow.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103858760B (en) * | 2014-02-26 | 2016-04-13 | 福建省农业科学院作物研究所 | The preparation method of a kind of roxburgh anoectochilus terminal bud test tube flowers |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN105900844B (en) * | 2016-05-13 | 2018-07-31 | 大连民族大学 | A kind of miniature ornamental plant and its cultural method |
| CN106016490A (en) * | 2016-07-13 | 2016-10-12 | 大连民族大学 | Ecotype air purifier |
| CN106386510A (en) * | 2016-12-06 | 2017-02-15 | 肇庆学院 | Manufacturing method of Anoectochilus roxburghii test tube flowers |
| CN107969340A (en) * | 2017-12-22 | 2018-05-01 | 绵阳师范学院 | A kind of culture medium and its cultural method of Fagus plant tissue culture |
| CN112042536A (en) * | 2020-09-09 | 2020-12-08 | 北京花乡花木集团有限公司 | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape |
| CN112335547A (en) * | 2020-11-11 | 2021-02-09 | 泉州市泉美生物科技有限公司 | Cultivation method of monarch tissue culture seedling micro-vase landscape |
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| CN201484047U (en) * | 2009-09-05 | 2010-05-26 | 浙江省农业科学院 | Miniature tissue-culture living-plant pendant ornament |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| USPP7030P (en) * | 1987-01-19 | 1989-09-12 | Philodendron Winterbourn |
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