CN107556119A - A kind of special nutrient fluid suitable for bottle plant thing - Google Patents
A kind of special nutrient fluid suitable for bottle plant thing Download PDFInfo
- Publication number
- CN107556119A CN107556119A CN201710999226.0A CN201710999226A CN107556119A CN 107556119 A CN107556119 A CN 107556119A CN 201710999226 A CN201710999226 A CN 201710999226A CN 107556119 A CN107556119 A CN 107556119A
- Authority
- CN
- China
- Prior art keywords
- plant
- bottle
- nutrient solution
- nutrient
- thing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a kind of special nutrient fluid suitable for bottle plant thing, solute is in the nutrient solution：Potassium nitrate, potassium dihydrogen phosphate, ammonium nitrate, epsom salt, peptone, Hua Bao, 6 BA, NAA, CCC, white sugar, agar；Part material is dissolved in distilled water successively in proportion, is diluted to set concentration, agar, the white sugar then added after heating and melting, pH value is adjusted to 5.8；Add phytochrome；Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C；By dendrobium candidum bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, lidstock, packaging.The invention provides a kind of bottle cultivation plant artwork for integrating and viewing and admiring and cultivating, periodic maintenance need not be carried out after plant is put into bottle, is not in lesion, health can be grown up strong and sturdy 23 years in bottle, and nutrient solution is lucuriant in design, there is certain ornamental value；The plant transplantation in bottle can additionally be come out at any time, be planted.
Description
Technical field
The invention belongs to field of plant growing technology, is related to a kind of nutrient solution, and being applied to bottle more particularly to one kind transplants
The special nutrient fluid of thing.
Background technology
With people's being on the increase, it is necessary to which horticultural production base provides more and better flowers to the green plant demand of flowers
Green plant, but traditional sowing and cuttage mode have not reached seedling quantity needed for production base, and time consuming, plant tissue
Culture is arisen at the historic moment.Plant Tissue Breeding is plant Sterile Culture Methods Used, also known as cultured in vitro, be had according to plant cell it is all-round
Property theory, by the in vitro organ of required plant (such as root, stem, leaf, stem apex, flower, fruit), tissue (such as forming layer, epidermis,
Cortex, marrow cell, endosperm etc.) or cell (such as megaspore, microspore, body cell) and plasm be seeded in containing various
Cultivated in the culture medium of nutriment and plant hormone, obtained the intact plant of regeneration.Some have economic value
Plant can also be used for large batch of industrialization production by way of Plant Tissue Breeding, to meet the needs of our markets.
But because most people lacks raise flowers experience and patience, many green plants of flowers often occur over time
It is slow-growing, wilt, rotten, it is even withered phenomena such as.The green plant of some flowers needs water planting, but water planting usually require it is larger
Container, not only taking up space is also easy to make the water in container to spill to bring unnecessary trouble, for being placed close to electrical equipment or space
Deficiency is absolutely unsafe in the case of have child.
The content of the invention
In order to solve problems of the prior art, the invention discloses a kind of special nutritional suitable for bottle plant thing
Liquid, it is desirable to provide a kind of bottle viewed and admired and cultivated that integrates plants plant artwork, need not be carried out after plant is put into bottle
Periodic maintenance, be not in lesion, health can be grown up strong and sturdy 2-3 in bottle, and nutrient solution is lucuriant in design, have certain
Ornamental value；The plant transplantation in bottle can additionally be come out at any time, be planted.
The present invention is achieved through the following technical solutions：
A kind of special nutrient fluid suitable for bottle plant thing, the nutrient solution includes following solute, and the solute is described
Concentration in nutrient solution is as follows：
Potassium nitrate（KNO3） 0.3-0.6g/L
Potassium dihydrogen phosphate（KH2PO4） 0.2-0.3g/L
Ammonium nitrate（NH4NO3） 0.45-0.55g/L
Epsom salt（MgSO4·7H2O） 0.27-0.3g/L
Peptone 0.8-1.5g/L
Spend precious 0.3-0.5g/L
6-BA 0.1-0.2mg/mL
NAA（Methyl α-naphthyl acetate） 0.05-0.1mg/mL
CCC（Cycocel） 0.5-1 mg/mL
White sugar 2.5-3.0 g/L
Agar 4.5-6g/L.
In order that its ornamental value is more preferably, vegetation color hormone, the vegetation color can also be included in the nutrient solution
Hormone is 1-2g/L.
Wherein, the bottle plant thing is the medicinal material of low consumption oxygen amount, preferably this kind of rare medicine of dendrobium candidum and/or roxburgh anoectochilus terminal bud
Material.
The effect that following table illustrates after changing nutrient solution prescription compares.
The effect of nutrient solution compares
。
The nutrient solution, which is made by the steps, to be formed：
（1）By KNO3、KH2PO4、NH4NO3、MgSO4·7H2O, peptone, Hua Bao, hormone are dissolved in distilled water successively in proportion
In, it is diluted to set concentration；
（2）Agar after heating and melting, white sugar are moved into step（1）In the solution of gained, pH value is adjusted to 5.8；
（3）Vegetation color hormone is added to step（2）In the solution of gained；
（4）Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C, and nutrient solution is moved in bottle；
（5）By plant bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, lidstock, bag
Dress；The plant bulb preferentially selects dendrobium candidum or roxburgh anoectochilus terminal bud bulb.
The method that plant is cultivated using above-mentioned special nutrient fluid, by plant bulb（It is preferred that dendrobium candidum or roxburgh anoectochilus terminal bud bulb）
It is inoculated into bottle, flourish is carried out in bottle sterile and being fully sealed, obtains bottle plant thing, wherein, the plant bulb
（It is preferred that dendrobium candidum or roxburgh anoectochilus terminal bud bulb）Inoculum concentration for 1 plant/（4-8）ML nutrient solutions；Preferably, plant bulb（It is preferred that iron
The skin stem of noble dendrobium or roxburgh anoectochilus terminal bud bulb）Inoculum concentration be 1 plant/5mL nutrient solutions.Bottle implants typically can health grow up strong and sturdy 2-3, and
Nutrient solution is lucuriant in design, and children are the growths that bottle implants are viewed and admired by vial, can not only learn the general knowledge of plant, also
Decoration function can be played, or relatives and friends are given as present.In order to improve aesthetics, bottle can be made as：Star,
The special-shaped bottles such as Little Bear, Doraemon, swan, four five-pointed stars, boots, are arranged in pairs or groups with the nutrient solution of different colours, bottle is transplanted thing
It is more attractive.
After bottle implants are grown up, the bottle can be transplanted thing and be transplanted, specific method for transplanting is by as follows
What step was realized：
（1）The bottle of bottle plant thing is opened, takes out plant；
（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；
（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot
It is in soil, soil pressure is real, 150-260mL water is poured, 200 mL water effects of watering every time are more preferable；
（4）A water was poured every 3-5 days, each 150-260mL, every time preferably watering 200mL are first 3 months, every to apply within 10-15 days
Fertilizer once, after three months, monthly applies fertilizer once, each dose is：The graceful fertilizers for potted flowers 3-5 grains of Fuller；
Whole environment temperature control is between 18-30 DEG C.
The present invention has the following advantages that compared with prior art：
（1）Plant seedling is not in entering directly into resident's hand after the stable Environment cultivation in tissue culture laboratory, and is also to again
Handled by hardening techniques such as nature alternating temperature sunshines, plant is more suitable for the complicated living environment of resident in itself；
（2）The nutrient solution that plant is absorbed in bottle can not only meet a bottle implants normal growth by developing meticulously
Needs, can also strengthen plant resistance to environment stress, coordinate hardening technique, dual fail-safe is provided for the extraneous complex environment of plant adaptation, with
Realization need not carry out periodic maintenance after plant is put into bottle, be not in lesion, health can be grown up strong and sturdy 2-3 in bottle
The target in year；
（3）Complete solar illuminating system is configured with, the release of its photosynthesis is increased while plant health is grown up
Oxygen, while bottle also add the air valve for being easy to people to breathe in itself, breathe oxygen-enriched caused by photosynthesis of plant；
（4）Plant in bottle, which has, selects very rare Chinese medicine（Dendrobium candidum, roxburgh anoectochilus terminal bud etc.）So that people can breathe richness
There is peculiar pleasant fragrant thoroughwort delicate fragrance while oxygen.
Embodiment
Embodiment 1
A kind of special nutrient fluid that dendrobium candidum is planted suitable for bottle, solute and its dense in the nutrient solution in the nutrient solution
Degree is as follows：
Potassium nitrate 0.6g/L
Potassium dihydrogen phosphate 0.2g/L
Ammonium nitrate 0.55g/L
Epsom salt 0.27g/L
Peptone 1.5g/L
Spend precious 0.3g/L
6-BA 0.2mg/mL
NAA 0.05mg/mL
CCC 1mg/mL
The g/L of white sugar 2.5
Agar 6g/L
Vegetation color hormone 2g/L.
The nutrient solution, which is made by the steps, to be formed：
（1）By KNO3、KH2PO4、NH4NO3、MgSO4·7H2O, peptone, Hua Bao, hormone are dissolved in distilled water successively in proportion
In, it is diluted to set concentration；
（2）Agar after heating and melting, white sugar are moved into step（1）In the solution of gained, pH value is adjusted to 5.8；
（3）Vegetation color hormone is added to step（2）In the solution of gained；
（4）Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C, and nutrient solution is moved in bottle；
（5）By dendrobium candidum bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, and capping is close
Envelope, packaging.
The method that plant is cultivated using above-mentioned special nutrient fluid, dendrobium candidum bulb is inoculated into bottle, sterile and complete
Flourish is carried out in hermetically sealed bottle, obtains bottle plant thing, wherein nutrient solution and inoculated plant amount is 1 plant/8mL nutrient solutions.
After bottle implants are grown up, the bottle can be transplanted thing and be transplanted, specific method for transplanting is by as follows
What step was realized：
（1）The bottle of bottle plant thing is opened, takes out plant；
（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；
（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot
It is in soil, soil pressure is real, pour 150mL water；
（4）A water was poured every 3 days, each 150mL, first 3 months, fertilising once, after three months, was monthly applied fertilizer per 10-15 days
Once, each dose is：The graceful fertilizers for potted flowers 3-5 grains of Fuller；
Whole environment temperature control is between 18-30 DEG C.
Embodiment 2
A kind of special nutrient fluid that roxburgh anoectochilus terminal bud is planted suitable for bottle, solute and its concentration in the nutrient solution in the nutrient solution
It is as follows：
Potassium nitrate 0.3g/L
Potassium dihydrogen phosphate 0.3g/L
Ammonium nitrate 0.45g/L
Epsom salt 0.3g/L
Peptone 0.8g/L
Spend precious 0.5g/L
6-BA 0.1mg/mL
NAA 0.1mg/mL
CCC 0.5 mg/mL
The g/L of white sugar 3.0
Agar 4.5g/L.
The nutrient solution, which is made by the steps, to be formed：
（1）By KNO3、KH2PO4、NH4NO3、MgSO4·7H2O, peptone, Hua Bao, hormone are dissolved in distilled water successively in proportion
In, it is diluted to set concentration；
（2）Agar after heating and melting, white sugar are moved into step（1）In the solution of gained, pH value is adjusted to 5.8；
（3）Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C, and nutrient solution is moved in bottle；
（4）By dendrobium candidum bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, and capping is close
Envelope, packaging.
The method that plant is cultivated using above-mentioned special nutrient fluid, roxburgh anoectochilus terminal bud bulb is inoculated into bottle, sterile and complete
Flourish is carried out in the bottle of sealing, bottle plant thing is obtained, wherein wherein nutrient solution and inoculated plant amount are 1 plant/5mL nutrition
Liquid.
After bottle implants are grown up, the bottle can be transplanted thing and be transplanted, specific method for transplanting is by as follows
What step was realized：
（1）The bottle of bottle plant thing is opened, takes out plant；
（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；
（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot
It is in soil, soil pressure is real, pour 260mL water；
（4）A water was poured every 5 days, each 260mL, first 3 months, fertilising once, after three months, was monthly applied fertilizer per 10-15 days
Once, each dose is：The graceful fertilizers for potted flowers 3-5 grains of Fuller；
Whole environment temperature control is between 18-30 DEG C.
Embodiment 3
A kind of special nutrient fluid that dendrobium candidum is planted suitable for bottle, solute and its dense in the nutrient solution in the nutrient solution
Degree is as follows：
Potassium nitrate 0.5g/L
Potassium dihydrogen phosphate 0.25g/L
Ammonium nitrate 0.50g/L
Epsom salt 0.28g/L
Peptone 0.8g/L
Spend precious 0.4g/L
6-BA 0.15mg/mL
NAA 0.08mg/mL
CCC 0.8mg/mL
The g/L of white sugar 2.8
Agar 5.6g/L
Vegetation color hormone 1g/L.
The nutrient solution, which is made by the steps, to be formed：
（1）By KNO3、KH2PO4、NH4NO3、MgSO4·7H2O, peptone, Hua Bao, hormone are dissolved in distilled water successively in proportion
In, it is diluted to set concentration；
（2）Agar after heating and melting, white sugar are moved into step（1）In the solution of gained, pH value is adjusted to 5.8；
（3）Vegetation color hormone is added to step（2）In the solution of gained；
（4）Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C, and nutrient solution is moved in bottle；
（5）By dendrobium candidum bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, and capping is close
Envelope, packaging.
The method that plant is cultivated using above-mentioned special nutrient fluid, dendrobium candidum bulb is inoculated into bottle, sterile and complete
Flourish is carried out in hermetically sealed bottle, obtains bottle plant thing, wherein nutrient solution and inoculated plant amount is 1 plant/4mL nutrient solutions.
After bottle implants are grown up, the bottle can be transplanted thing and be transplanted, specific method for transplanting is by as follows
What step was realized：
（1）The bottle of bottle plant thing is opened, takes out plant；
（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；
（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot
It is in soil, soil pressure is real, pour 200mL water；
（4）A water was poured every 3 days, each 200mL, first 3 months, fertilising once, after three months, was monthly applied fertilizer per 10-15 days
Once, each dose is：The graceful fertilizers for potted flowers 3-5 grains of Fuller；
Whole environment temperature control is between 18-30 DEG C.
Bottle implants in embodiment 1 ~ 3 are healthy after removing to grow vigorously.
Claims (10)
- A kind of 1. special nutrient fluid suitable for bottle plant thing, it is characterised in that：The nutrient solution includes following solute, and institute It is as follows to state concentration of the solute in the nutrient solution：KNO3 0.3-0.6g/LKH2PO4 0.2-0.3g/LNH4NO3 0.45-0.55g/LMgSO4·7H2O 0.27-0.3g/LPeptone 0-1.5g/LSpend precious 0.3-0.5g/L6-BA 0.1-0.2mg/mLNAA 0.05-0.1mg/mLCCC 0.5-1.0 mg/mLWhite sugar 2.5-3.0 g/LAgar 4.5-6.0g/L.
- 2. special nutrient fluid as claimed in claim 1, it is characterised in that：Also include vegetation color hormone in the nutrient solution, The vegetation color hormone is 1-2g/L.
- 3. special nutrient fluid as claimed in claim 1, it is characterised in that：The bottle plant thing is the medicinal material of low consumption oxygen amount.
- 4. special nutrient fluid as claimed in claim 1, it is characterised in that：The bottle plant thing is dendrobium candidum or roxburgh anoectochilus terminal bud.
- 5. a kind of preparation method suitable for bottle plant thing special nutrient fluid, it is characterised in that be achieved by the steps of：（1）By KNO3、KH2PO4、NH4NO3、MgSO4·7H2O, peptone, Hua Bao, hormone are dissolved in distilled water successively in proportion In, it is diluted to set concentration；（2）Agar after heating and melting, white sugar are moved into step（1）In the solution of gained, pH value is adjusted to 5.8；（3）Vegetation color hormone is added to step（2）In the solution of gained；（4）Sterilization treatment 20min obtains nutrient solution under the conditions of 121 DEG C, and nutrient solution is moved in bottle；（5）By plant bulb after inducing, breed, break up, selection is of good performance to be inoculated into bottle, lidstock, bag Dress；Wherein, the inoculum concentration of the plant bulb be 1 plant/（4-8）ML nutrient solutions, it is preferable that 1 plant/5mL nutrient solutions.
- 6. preparation method as claimed in claim 5, it is characterised in that：The plant bulb is dendrobium candidum or roxburgh anoectochilus terminal bud ball Stem.
- 7. a kind of method that any special nutrient fluid cultivates plant in 1-4 using claim, plant bulb is inoculated into In bottle, flourish is carried out in bottle sterile and being fully sealed, obtains bottle plant thing, wherein, the inoculation of the plant bulb Measure for 1 plant/（4-8）ML nutrient solutions.
- 8. method as claimed in claim 5, it is characterised in that：The inoculum concentration of the plant bulb is 1 plant/5mL nutrient solutions.
- 9. bottle described in a kind of claim 7 or 8 transplants the method for transplanting of thing, it is characterised in that is to be achieved by the steps of 's：（1）The bottle of bottle plant thing is opened, takes out plant；（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot It is in soil, soil pressure is real, pour 150-260mL water；（4）A water was poured every 3-5 days, each 150-260mL, first 3 months, was applied fertilizer once per 10-15 days, after three months, often Once, each dose is for moon fertilising：The graceful fertilizers for potted flowers 3-5 grains of Fuller；Whole environment temperature control is between 18-30 DEG C.
- 10. method for transplanting as claimed in claim 9, it is characterised in that：It is achieved by the steps of：（1）The bottle of bottle plant thing is opened, takes out plant；（2）The nutrient solution of plant root is cleaned up, shady place is placed on and dries, be advisable with root without nutrient solution；（3）Appropriate soil is placed into flowerpot, step will be passed through（2）Root is immersed in by the planting of processing entirely in flowerpot It is in soil, soil pressure is real, pour 200mL water；（4）A water was poured every 3-5 days, each 200mL, first 3 months, fertilising once, after three months, was monthly applied per 10-15 days Once, each dose is fertilizer：The graceful fertilizers for potted flowers 3-5 grains of Fuller；Whole environment temperature control is between 18-30 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999226.0A CN107556119A (en) | 2017-10-24 | 2017-10-24 | A kind of special nutrient fluid suitable for bottle plant thing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710999226.0A CN107556119A (en) | 2017-10-24 | 2017-10-24 | A kind of special nutrient fluid suitable for bottle plant thing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107556119A true CN107556119A (en) | 2018-01-09 |
Family
ID=60986008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710999226.0A Pending CN107556119A (en) | 2017-10-24 | 2017-10-24 | A kind of special nutrient fluid suitable for bottle plant thing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107556119A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| CN101278646A (en) * | 2008-05-26 | 2008-10-08 | 福建农林大学 | Method for producing sterilized miniascape |
| CN102050653A (en) * | 2010-10-15 | 2011-05-11 | 天津滨海国际花卉科技园区股份有限公司 | Zygocactus cultivation nutrient solution and cultivation method thereof |
| CN102499074A (en) * | 2011-09-30 | 2012-06-20 | 杭州鼎韵农业开发有限公司 | Tissue culture medium for anoectochilus roxburghii |
| CN103011969A (en) * | 2012-12-25 | 2013-04-03 | 浙江农林大学 | Medium specialized for plant tissue culture, and preparation method thereof |
| CN103918551A (en) * | 2014-01-16 | 2014-07-16 | 贵州清控置业有限公司 | Imitation wild cultivation method of mycorrhizated dendrobium plant tea tree |
| CN103931496A (en) * | 2014-04-02 | 2014-07-23 | 河南省南阳农业学校 | Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN104982196A (en) * | 2015-06-26 | 2015-10-21 | 江苏绿苑园林建设有限公司 | Cultivation method for accelerating growth speed of nandina domestica 'Fire power' |
| CN105104209A (en) * | 2015-09-24 | 2015-12-02 | 戴亚峰 | Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method |
| CN106386510A (en) * | 2016-12-06 | 2017-02-15 | 肇庆学院 | Manufacturing method of Anoectochilus roxburghii test tube flowers |
| CN107047305A (en) * | 2017-04-26 | 2017-08-18 | 福建省农业科学院作物研究所 | A kind of quick breeding method for tissue culture of dendrobium seedling |
| CN110178729A (en) * | 2019-05-20 | 2019-08-30 | 南阳师范学院 | A kind of process flow of in vitro plant text wound product |
-
2017
- 2017-10-24 CN CN201710999226.0A patent/CN107556119A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374237A (en) * | 2001-03-14 | 2002-10-16 | 杭州泛邦植物细胞工程有限公司 | Closed composite plant enjoying container |
| CN101278646A (en) * | 2008-05-26 | 2008-10-08 | 福建农林大学 | Method for producing sterilized miniascape |
| CN102050653A (en) * | 2010-10-15 | 2011-05-11 | 天津滨海国际花卉科技园区股份有限公司 | Zygocactus cultivation nutrient solution and cultivation method thereof |
| CN102499074A (en) * | 2011-09-30 | 2012-06-20 | 杭州鼎韵农业开发有限公司 | Tissue culture medium for anoectochilus roxburghii |
| CN103011969A (en) * | 2012-12-25 | 2013-04-03 | 浙江农林大学 | Medium specialized for plant tissue culture, and preparation method thereof |
| CN103918551A (en) * | 2014-01-16 | 2014-07-16 | 贵州清控置业有限公司 | Imitation wild cultivation method of mycorrhizated dendrobium plant tea tree |
| CN103931496A (en) * | 2014-04-02 | 2014-07-23 | 河南省南阳农业学校 | Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method |
| CN104094854A (en) * | 2014-07-30 | 2014-10-15 | 陈小超 | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof |
| CN104982196A (en) * | 2015-06-26 | 2015-10-21 | 江苏绿苑园林建设有限公司 | Cultivation method for accelerating growth speed of nandina domestica 'Fire power' |
| CN105104209A (en) * | 2015-09-24 | 2015-12-02 | 戴亚峰 | Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method |
| CN106386510A (en) * | 2016-12-06 | 2017-02-15 | 肇庆学院 | Manufacturing method of Anoectochilus roxburghii test tube flowers |
| CN107047305A (en) * | 2017-04-26 | 2017-08-18 | 福建省农业科学院作物研究所 | A kind of quick breeding method for tissue culture of dendrobium seedling |
| CN110178729A (en) * | 2019-05-20 | 2019-08-30 | 南阳师范学院 | A kind of process flow of in vitro plant text wound product |
Non-Patent Citations (8)
| Title |
|---|
| 史宗义: "《养兰实用全书》", 31 January 2015, 福建科学出版社 * |
| 张之鹏等: "不同营养液对铁皮石斛生长及多糖含量的影响 ", 《南昌大学学报(理科版)》 * |
| 张传海等: "《植物生物技术》", 30 September 2015, 合肥工业大学出版社 * |
| 朱建华等: "《植物组织培养实用技术》", 31 January 2002, 中国计量出版社 * |
| 王运兵: "《无公害农药实用手册》", 30 September 2010, 河南科学技术出版社 * |
| 谢树云等: "铁皮石斛无土栽培技术的探讨 ", 《中国科技信息》 * |
| 郑志新等: "栽植方式和营养液对铁皮石斛试管苗移栽成活和生长的影响 ", 《安徽农业科学》 * |
| 陈忠辉: "《农业生物技术》", 28 February 2002, 高等教育出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101587707B1 (en) | Producing method of orchid seedlings | |
| CN105284620B (en) | A kind of method that Superearly peach bybrid embryo saves seedling | |
| CN105103714B (en) | Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f. | |
| CN106561456B (en) | A kind of Helen's pocket orchid aseptic seeding rapid propagation method | |
| CN101946709B (en) | Seedling raising method of plateau rhodiola crenulata | |
| CN102823503B (en) | Tissue culture medium for propagating anthurium buds by using buds | |
| CN104542281B (en) | Mediterranean Jia Opulus tissue culture propagation method | |
| CN104221827A (en) | Direct transplanting and soilless planting method for tissue culture seedlings of dendrobium officinale | |
| CN108293861A (en) | A kind of spring greenhouse small watermelon mixed-matrix soilless culture method | |
| CN107258299A (en) | The flower promoting method and cultural method of a kind of Bougainvillea spectabilis | |
| CN106804428B (en) | A kind of Cremastra appendiculata method for culturing seedlings | |
| CN104823799A (en) | Grape root zone limiting cultivating method | |
| CN104094854A (en) | In-vivo ornamental plant in closed transparent container, and manufacturing method thereof | |
| CN107624634A (en) | A kind of method of black chrysanthemum seed growing | |
| CN107333558A (en) | A kind of packed roxburgh anoectochilus terminal bud implantation methods of sealing | |
| CN107466850B (en) | Blueberry plantation and its seedling fast breeding method | |
| CN110122333A (en) | A kind of method that flame tree seed stratification is taken root | |
| CN105309291A (en) | Taxus X media hydroponic potted landscape and manufacturing method thereof | |
| CN107556119A (en) | A kind of special nutrient fluid suitable for bottle plant thing | |
| CN107494269A (en) | A kind of elimination little Hua all ages in blue tissue culture procedures endophyte method | |
| CN112042536A (en) | Construction method of tissue culture in-bottle micro landscape and tissue culture in-bottle micro landscape | |
| CN111742781A (en) | Efficient and practical Chinese chestnut ectomycorrhiza soil basin inoculation system and application thereof | |
| CN1973618B (en) | Tissue culture process of common lophatherum herb | |
| CN110301335A (en) | The cultivation of the lateral bud breeding bulb of west safflower | |
| CN109392619A (en) | Seedling raising method of plateau rhodiola crenulata |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180109 |
|
| RJ01 | Rejection of invention patent application after publication |