CN106804428B - A kind of Cremastra appendiculata method for culturing seedlings - Google Patents
A kind of Cremastra appendiculata method for culturing seedlings Download PDFInfo
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- CN106804428B CN106804428B CN201710052795.4A CN201710052795A CN106804428B CN 106804428 B CN106804428 B CN 106804428B CN 201710052795 A CN201710052795 A CN 201710052795A CN 106804428 B CN106804428 B CN 106804428B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of Cremastra appendiculata method for culturing seedlings that can greatly shorten growing-seedling period.The Cremastra appendiculata method for culturing seedlings includes seed selection, prepares culture medium, inoculation, culturing room's culture, hardening, bottle outlet cultivation, fertilising, transplants, the present invention improves tissue culture and hardening off method and process, and it develops and just starts the culture medium prescription taken root in the tissue culture first stage, next nearly 3 months tissue culture time is omitted, the switching process of tissue-cultured seedling twice is eliminated simultaneously, a large amount of manpower financial capacity is saved, the sufficient time is provided for the production of next group Cremastra appendiculata seedling.It is suitble to promote the use in biotechnology.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of Cremastra appendiculata method for culturing seedlings.
Background technology
Cremastra appendiculata is orchid family cuckoo Cymbidium herbaceos perennial.Claim edible tulip or hair arrowhead after the drying of its pseudobulb, it can
It is used as medicine, attaches most importance to and want Chinese medicine in short supply.Modern medicine study shows to use in edible tulip, and there is antitumor action, external application to control sore, worm
Snake bite, skin scald or burn etc..Since people rob formula excavation for a long time, self germ mechanism limits in addition, causes resource
It is exhausted.As medicinal material market increases Cremastra appendiculata demand, Cremastra appendiculata has quickly been multiplied into problem urgently to be resolved hurrily.Later,
Researcher has developed the cultivation Cremastra appendiculata seedling under sterile tissue condition of culture, the disadvantage is that process is cumbersome, long time period,
It is of high cost.
Invention content
Technical problem to be solved by the invention is to provide a kind of Cremastra appendiculata nursery sides that can greatly shorten growing-seedling period
Method.
The technical solution adopted by the present invention to solve the technical problems is：The Cremastra appendiculata method for culturing seedlings, includes the following steps：
A, it chooses seeds：Cremastra appendiculata pod seed is chosen, and is carried out disinfection processing to Cremastra appendiculata pod seed；
B, culture medium is prepared：The culture medium is made of 1/2~1MS, 2.5~4.5g/L agar, 0~0.1mg/LNAA,
The pH value of the culture medium is 5.8, and the culture medium prepared is fitted into tissue culture bottle；
C, it is inoculated with：It will be uniformly sprinkling upon on the culture medium in tissue culture bottle by the Cremastra appendiculata pod seed disinfected；
D, culturing room cultivates：It is 1500~3000lx, temperature 25 that tissue culture bottle after will be inoculated, which is placed on intensity of illumination,
30~60d is cultivated in~30 DEG C of gnotobasis, light application time is 8~12h/d；
E, hardening：After culturing room cultivates, it is that 20~30 DEG C have in collarium border cultivates 4 that tissue culture bottle, which is placed on temperature,
~7d；
F, bottle outlet is cultivated：The seedling in tissue culture bottle is taken out and cleaned up after hardening, is then transplanted in greenhouse
Seed plate in；
G, it applies fertilizer：It is transplanted to seed plate and sprays foliar fertilizer after a week, and monthly apply since being transplanted to after seed plate quantitative
Composite fertilizer；
H, it transplants：Bottle outlet is transplanted in natural land after cultivating 3~6 months and is cultivated.
Further, in stepb, the culture medium is made of 1/2MS, 4g/L agar, 0.01mg/LNAA.
Further, in step D, will be inoculated after tissue culture bottle to be placed on intensity of illumination be 2000lx, temperature is
45d, light application time 10h/d are cultivated in 25 DEG C of gnotobasis.
Further, in step F, upper layer and lower layer soil is equipped in the seed plate, the lower soil is nutrition
The weight ratio of the mixture of soil and farm manure, the Nutrition Soil and farm manure is 1：1, the upper layer of soil is coconut palm chaff, the seedling
The pH value of soil is 5.5~6.5 in disk.
Further, the thickness of the lower soil is 3cm, the thickness of the upper layer of soil is 2cm.
Further, in stepb, preparing culture medium is carried out in gnotobasis, in step C, is seeded in nothing
It is carried out in collarium border.
Further, the MS culture mediums include KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2·2H2O、
MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、
FeSO4·4H2O, the content of glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin, inositol, sucrose, each component is as described below：
KNO3For 1900mg/L, NH4NO3For 1650mg/L, MgSO47H2O is 370mg/L, KH2PO4For 170mg/L, CaCl2·2H2O
For 440mg/L, MnSO4·4H2O is 22.3mg/L, ZnSO4·7H2O is 8.6mg/L, H3BO3For 6.2mg/L, KI 0.83mg/
L, NaMoO4·2H2O is 0.25mg/L, CuSO4·5H2O is 0.025mg/L, CoCl2·6H2O is 0.025mg/L, Na2-EDTA
For 37.3mg/L, FeSO4·4H2O is 27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.1mg/L, and hydrochloric acid pyrrole is trembled
Alcohol is 0.5mg/L, niacin 0.5mg/L, inositol 100mg/L, sucrose 30g/L.
Further, the 1/2MS culture mediums include KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2·
2H2O、MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-
EDTA、FeSO4·4H2O, the content of glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin, inositol, sucrose, each component is as follows
It is described：KNO3For 950mg/L, NH4NO3For 825mg/L, MgSO47H2O is 185mg/L, KH2PO4For 85mg/L, CaCl2·
2H2O is 220mg/L, MnSO4·4H2O is 11.15mg/L, ZnSO4·7H2O is 4.3mg/L, H3BO3It is for 3.1mg/L, KI
0.415mg/L, NaMoO4·2H2O is 0.125mg/L, CuSO4·5H2O is 0.0125mg/L, CoCl2·6H2O is
0.0125mg/L, Na2- EDTA is 37.3mg/L, FeSO4·4H2O is 27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride
For 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, inositol 100mg/L, sucrose 30g/L.
The beneficial effects of the present invention are：The Cremastra appendiculata method for culturing seedlings includes seed selection, prepares culture medium, inoculation, culturing room
Culture, hardening, bottle outlet are cultivated, fertilising, are transplanted, and the present invention improves tissue culture and hardening off method and process, and develops
The tissue culture first stage just starts the culture medium prescription taken root, and next nearly 3 months tissue culture time is omitted, saves simultaneously
The switching process of tissue-cultured seedling twice, saves a large amount of manpower financial capacity, is provided for the production of next group Cremastra appendiculata seedling sufficient
Time.
Specific implementation mode
Cremastra appendiculata method for culturing seedlings of the present invention, includes the following steps：
A, it chooses seeds：Cremastra appendiculata pod seed is chosen, and is carried out disinfection processing to Cremastra appendiculata pod seed；
B, culture medium is prepared：The culture medium is made of 1/2~1MS, 2.5~4.5g/L agar, 0~0.1mg/LNAA,
The pH value of the culture medium is 5.8, and the culture medium prepared is fitted into tissue culture bottle；
C, it is inoculated with：It will be uniformly sprinkling upon on the culture medium in tissue culture bottle by the Cremastra appendiculata pod seed disinfected；
D, culturing room cultivates：It is 1500~3000lx, temperature 25 that tissue culture bottle after will be inoculated, which is placed on intensity of illumination,
30~60d is cultivated in~30 DEG C of gnotobasis, light application time is 8~12h/d；
E, hardening：After culturing room cultivates, it is that 20~30 DEG C have in collarium border cultivates 4 that tissue culture bottle, which is placed on temperature,
~7d；
F, bottle outlet is cultivated：The seedling in tissue culture bottle is taken out and cleaned up after hardening, is then transplanted in greenhouse
Seed plate in；
G, it applies fertilizer：It is transplanted to seed plate and sprays foliar fertilizer after a week, and monthly apply since being transplanted to after seed plate quantitative
Composite fertilizer；
H, it transplants：Bottle outlet is transplanted in natural land after cultivating 3~6 months and is cultivated.
The Cremastra appendiculata method for culturing seedlings includes seed selection, prepares culture medium, inoculation, culturing room's culture, hardening, bottle outlet cultivation, applies
Fertilizer, transplanting, the present invention improves tissue culture and hardening off method and process, and develops and just start in the tissue culture first stage
The culture medium prescription taken root, is omitted next nearly 3 months tissue culture time, while eliminating the switching of tissue-cultured seedling twice
Journey saves a large amount of manpower financial capacity, and the sufficient time is provided for the production of next group Cremastra appendiculata seedling.
During above-mentioned Cremastra appendiculata method for culturing seedlings, in stepb, the culture medium by 1/2MS, 4g/L agar,
0.01mg/LNAA is formed.The culture medium of the proportioning is specific to Cremastra appendiculata research and development, and rooting efficiency is preferable, wherein 4g/L
Agar so that culture medium is in half solidification semi liquid state state, so that the seedling that sprouting goes out is easily isolated with culture medium, be convenient for the later stage
Transplanting.
In order to promote Cremastra appendiculata to root, in step D, will be inoculated after tissue culture bottle be placed on intensity of illumination and be
2000lx, temperature are to cultivate 45d, light application time 10h/d in 25 DEG C of gnotobasis.
Further, in step F, upper layer and lower layer soil is equipped in the seed plate, the lower soil is nutrition
The weight ratio of the mixture of soil and farm manure, the Nutrition Soil and farm manure is 1：1, the upper layer of soil is coconut palm chaff, the seedling
The pH value of soil is 5.5~6.5 in disk.Soil matrix in the seed plate be specific to immature Cremastra appendiculata seedling root growth and its
The soil matrix of development mainly realizes two layers of distribution in soil matrix, and the coconut palm chaff on upper layer can keep moisture, suitable for children
The root growth of seedling, the Nutrition Soil of the lower layer and mixture of farm manure is lasting provides nutrient for Cremastra appendiculata seedling, and by pH value control
System can allow immature tissue-cultured seedling smoothly to be grown up between 5.5~6.5, and this method can produce Cremastra appendiculata seedling in batches in the short time.For
The thickness of the further growth for promoting Cremastra appendiculata seedling, the lower soil is 3cm, and the thickness of the upper layer of soil is 2cm.
In order to avoid the infection of extraneous bacterium, in stepb, preparing culture medium is carried out in gnotobasis, in step C
In, it is seeded in gnotobasis and carries out.
Further, the MS culture mediums include KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2·2H2O、
MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、
FeSO4·4H2O, the content of glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin, inositol, sucrose, each component is as described below：
KNO3For 1900mg/L, NH4NO3For 1650mg/L, MgSO47H2O is 370mg/L, KH2PO4For 170mg/L, CaCl2·2H2O
For 440mg/L, MnSO4·4H2O is 22.3mg/L, ZnSO4·7H2O is 8.6mg/L, H3BO3For 6.2mg/L, KI 0.83mg/
L, NaMoO4·2H2O is 0.25mg/L, CuSO4·5H2O is 0.025mg/L, CoCl2·6H2O is 0.025mg/L, Na2-EDTA
For 37.3mg/L, FeSO4·4H2O is 27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.1mg/L, and hydrochloric acid pyrrole is trembled
Alcohol is 0.5mg/L, niacin 0.5mg/L, inositol 100mg/L, sucrose 30g/L.This formula is specific to Cremastra appendiculata seedling
Configuration, the tissue cultures more suitable for Cremastra appendiculata seedling.
Further, the 1/2MS culture mediums include KNO3、NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2·
2H2O、MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-
EDTA、FeSO4·4H2O, the content of glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin, inositol, sucrose, each component is as follows
It is described：KNO3For 950mg/L, NH4NO3For 825mg/L, MgSO47H2O is 185mg/L, KH2PO4For 85mg/L, CaCl2·
2H2O is 220mg/L, MnSO4·4H2O is 11.15mg/L, ZnSO4·7H2O is 4.3mg/L, H3BO3It is for 3.1mg/L, KI
0.415mg/L, NaMoO4·2H2O is 0.125mg/L, CuSO4·5H2O is 0.0125mg/L, CoCl2·6H2O is
0.0125mg/L, Na2- EDTA is 37.3mg/L, FeSO4·4H2O is 27.8mg/L, glycine 2.0mg/L, thiamine hydrochloride
For 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, inositol 100mg/L, sucrose 30g/L.This formula
It is specific to the configuration of Cremastra appendiculata seedling, the tissue cultures more suitable for Cremastra appendiculata seedling.
Embodiment 1：
A, it chooses seeds：Cremastra appendiculata pod seed is chosen, and is carried out disinfection processing to Cremastra appendiculata pod seed in gnotobasis；
B, culture medium is prepared：The culture medium is made of 1/2MS, 4g/L agar, 0.01mg/LNAA, the culture medium
PH value is 5.8, and the culture medium prepared is fitted into tissue culture bottle；
C, it is inoculated with：It will be uniformly sprinkling upon on the culture medium in tissue culture bottle by the Cremastra appendiculata pod seed disinfected；
D, culturing room cultivates：It is 2000lx, the nothing that temperature is 25 DEG C that tissue culture bottle after will be inoculated, which is placed on intensity of illumination,
45d, light application time 10h/d are cultivated in collarium border；
E, hardening：After culturing room cultivates, seedling is observed at this time and grows the root of two 1cm or so, when two panels leaf
7d in the greenhouse of outdoor without sunlight direct projection is placed it in, temperature is 20~30 DEG C；
F, bottle outlet is cultivated：The seedling in tissue culture bottle is taken out and cleaned up after hardening, is then transplanted in greenhouse
Seed plate in；Upper layer and lower layer soil is equipped in the seed plate, the lower soil is the mixture of Nutrition Soil and farm manure, institute
The weight ratio for stating Nutrition Soil and farm manure is 1：1, the upper layer of soil is coconut palm chaff, and the thickness of the lower soil is 3cm, described
The thickness of upper layer of soil is 2cm, and the pH value of soil is adjusted using lime to 6.2 in the seed plate, and in the whole process, soil is answered
Moistening is kept at any time；
G, it applies fertilizer：It is transplanted to seed plate and sprays foliar fertilizer after a week, and monthly apply since being transplanted to after seed plate quantitative
Composite fertilizer；
H, it transplants：Bottle outlet is transplanted in natural land after cultivating 3~6 months and is cultivated.
In step A-D, Cremastra appendiculata seed disinfection and the young plant for sprouting formation are carried out under sterile environment, in step
In rapid E-H, Cremastra appendiculata carries out in the environment for having bacterium.
Embodiment 2：
A, it chooses seeds：Cremastra appendiculata pod seed is chosen, and is carried out disinfection processing to Cremastra appendiculata pod seed in gnotobasis；
B, culture medium is prepared：The culture medium is made of MS, 4g/L agar, 0mg/LNAA, and the pH value of the culture medium is
5.8, the culture medium prepared is fitted into tissue culture bottle；
C, it is inoculated with：It will be uniformly sprinkling upon on the culture medium in tissue culture bottle by the Cremastra appendiculata pod seed disinfected；
D, culturing room cultivates：It is 2000lx, the nothing that temperature is 27 DEG C that tissue culture bottle after will be inoculated, which is placed on intensity of illumination,
45d, light application time 12h/d are cultivated in collarium border；
E, hardening：After culturing room cultivates, seedling is observed at this time and grows the root of two 1cm or so, when two panels leaf
4d in the greenhouse of outdoor without sunlight direct projection is placed it in, temperature is 20~30 DEG C；
F, bottle outlet is cultivated：The seedling in tissue culture bottle is taken out and cleaned up after hardening, is then transplanted in greenhouse
Seed plate in；Upper layer and lower layer soil is equipped in the seed plate, the lower soil is the mixture of Nutrition Soil and farm manure, institute
The weight ratio for stating Nutrition Soil and farm manure is 1：1, the upper layer of soil is coconut palm chaff, and the thickness of the lower soil is 3cm, described
The thickness of upper layer of soil is 2cm, and the pH value of soil is adjusted using lime to 5.5 in the seed plate, and in the whole process, soil is answered
Moistening is kept at any time；
G, it applies fertilizer：It is transplanted to seed plate and sprays foliar fertilizer after a week, and monthly apply since being transplanted to after seed plate quantitative
Composite fertilizer；
H, it transplants：Bottle outlet is transplanted in natural land after cultivating 3~6 months and is cultivated.
Claims (8)
1. a kind of Cremastra appendiculata method for culturing seedlings, it is characterised in that include the following steps：
A, it chooses seeds：Cremastra appendiculata pod seed is chosen, and is carried out disinfection processing to Cremastra appendiculata pod seed；
B, culture medium is prepared：The culture medium is made of 1/2~1MS, 2.5~4.5g/L agar, 0~0.1mg/LNAA, described
The pH value of culture medium is 5.8, and the culture medium prepared is fitted into tissue culture bottle；
C, it is inoculated with：It will be uniformly sprinkling upon on the culture medium in tissue culture bottle by the Cremastra appendiculata pod seed disinfected；
D, culturing room cultivates：It is 1500~3000lx that tissue culture bottle after will be inoculated, which is placed on intensity of illumination, and temperature is 25~30
DEG C gnotobasis in cultivate 30~60d, light application time be 8~12h/d；
E, hardening：After culturing room cultivates, by tissue culture bottle be placed on temperature be 20~30 DEG C have in collarium border culture 4~
7d；
F, bottle outlet is cultivated：The seedling in tissue culture bottle is taken out and cleaned up after hardening, the seedling being then transplanted in greenhouse
In disk；
G, it applies fertilizer：It is transplanted to seed plate and sprays foliar fertilizer after a week, and monthly apply since being transplanted to after seed plate quantitative compound
Fertilizer；
H, it transplants：Bottle outlet is transplanted in natural land after cultivating 3~6 months and is cultivated.
2. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：In stepb, the culture medium by 1/2MS,
4g/L agar, 0.01mg/LNAA compositions.
3. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：In step D, the tissue culture bottle after will be inoculated
It is 2000lx to be placed on intensity of illumination, and 45d, light application time 10h/d are cultivated in the gnotobasis that temperature is 25 DEG C.
4. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：In step F, it is equipped in the seed plate
Lower Two layer soil, the lower soil are the mixture of Nutrition Soil and farm manure, and the weight ratio of the Nutrition Soil and farm manure is
1：1, the upper layer of soil is coconut palm chaff, and the pH value of soil is 5.5~6.5 in the seed plate.
5. Cremastra appendiculata method for culturing seedlings as claimed in claim 4, it is characterised in that：The thickness of the lower soil is 3cm, described
The thickness of upper layer of soil is 2cm.
6. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：In stepb, it is sterile to prepare culture medium
It is carried out in environment, in step C, is seeded in gnotobasis and carries out.
7. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：The MS culture mediums include KNO3、NH4NO3、
MgSO4·7H2O、KH2PO4、CaCl2·2H2O、MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·2H2O、
CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、FeSO4·4H2O, glycine, thiamine hydrochloride, puridoxine hydrochloride, niacin,
The content of inositol, sucrose, each component is as described below：KNO3For 1900mg/L, NH4NO3For 1650mg/L, MgSO47H2O is
370mg/L, KH2PO4For 170mg/L, CaCl2.2H2O is 440mg/L, MnSO4·4H2O is 22.3mg/L, ZnSO4·7H2O is
8.6mg/L, H3BO3For 6.2mg/L, KI 0.83mg/L, NaMoO4·2H2O is 0.25mg/L, CuSO4·5H2O is
0.025mg/L, CoCl2·6H2O is 0.025mg/L, Na2- EDTA is 37.3mg/L, FeSO4·4H2O is 27.8mg/L, sweet ammonia
Acid is 2.0mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, and niacin 0.5mg/L, inositol is
100mg/L, sucrose 30g/L.
8. Cremastra appendiculata method for culturing seedlings as described in claim 1, it is characterised in that：The 1/2MS culture mediums include KNO3、
NH4NO3、MgSO4·7H2O、KH2PO4、CaCl2.2H2O、MnSO4·4H2O、ZnSO4·7H2O、H3BO3、KI、NaMoO4·
2H2O、CuSO4·5H2O、CoCl2·6H2O、Na2-EDTA、FeSO4·4H2O, glycine, thiamine hydrochloride, puridoxine hydrochloride,
The content of niacin, inositol, sucrose, each component is as described below：KNO3For 950mg/L, NH4NO3For 825mg/L, MgSO47H2O
For 185mg/L, KH2PO4For 85mg/L, CaCl2.2H2O is 220mg/L, MnSO4·4H2O is 11.15mg/L, ZnSO4·7H2O
For 4.3mg/L, H3BO3For 3.1mg/L, KI 0.415mg/L, NaMoO4·2H2O is 0.125mg/L, CuSO4·5H2O is
0.0125mg/L, CoCl2·6H2O is 0.0125mg/L, Na2- EDTA is 37.3mg/L, FeSO4·4H2O is 27.8mg/L, sweet
Propylhomoserin is 2.0mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, and inositol is
100mg/L, sucrose 30g/L.
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| CN110771486B (en) * | 2019-11-14 | 2022-03-01 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Seedling hardening method for tissue culture seedlings of hybrid orchid |
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Effective date of registration: 20190419 Address after: 611130 No. 203, 204 and 205, No. 355 Kejin Road, Wenjiang District, Chengdu City, Sichuan Province Patentee after: Chengdu Metropolitan Modern Agricultural Industry Technology Research Institute Co., Ltd. Address before: 611130 Huimin Road, Wenjiang District, Chengdu, Sichuan Province, No. 211 Patentee before: Sichuan Agricultural University |
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