CN106888950A - A kind of agarose gel matrix and preparation method thereof - Google Patents
A kind of agarose gel matrix and preparation method thereof Download PDFInfo
- Publication number
- CN106888950A CN106888950A CN201710098767.6A CN201710098767A CN106888950A CN 106888950 A CN106888950 A CN 106888950A CN 201710098767 A CN201710098767 A CN 201710098767A CN 106888950 A CN106888950 A CN 106888950A
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- agarose gel
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of agarose gel matrix and preparation method thereof, the preparation method includes:By water, agar powder and inorganic salts mix, by the first mixture heating stirring to boil and agar powder and inorganic salts dissolving, obtain the first solution;The pH value of the first solution is adjusted, the first solution is poured into container, solid gel shape is cooled at room temperature after removing bubble, obtain the first nutritive layer;Water and agar powder are mixed, the second mixture heating stirring to agar powder is dissolved, obtained the second solution;Adjust the pH value of the second solution;It is poured on after second solution is cooled down at room temperature on the first nutritive layer, solid gel shape is continued cool at room temperature after removing bubble, obtains the second nutritive layer.The agarose gel matrix that the present invention is obtained is used for plant soil-less culturing, do not spilt with matrix, user can watch the root system of cultivated plant by matrix, cycle of watering is flexible, without often adding or changing nutritional ingredient, Consumer's Experience, and repeatable utilization are improve, it is nontoxic, the characteristics of low cost.
Description
Technical field
The present invention relates to plant soil-less culturing technical field, more particularly to a kind of agarose gel matrix and its preparation side
Method.
Background technology
Potted plant is widely deployed in daily life as interior decoration.In order to allow plant preferably to grow, lead to
Often need in potted plant interior addition culture matrix, and from for the type of culture matrix, potted plant is generally divided into tradition native cultivation
Training and the major class of soilless culture two, wherein, soilless culture is primarily referred to as directly cultivating potted plant with nutrient solution, with often benefit
The shortcomings of filling or change nutrient solution, liquid and easily splash.
In recent years, gel-type vehicle is gradually applied to potted plant soilless culture field, typically solidifying with polyacrylamide
Glue or high hydroscopic resin gel particle as transparent cultivation matrix, in soilless culture of flowers, flower arrangement, fancy horticulture.But it is poly-
The unpolymerized acrylamide remained in acrylamide gel is toxic to human nerve, if long-term use can endanger the mankind and be good for
Health;And high hydroscopic resin gel particle has extremely strong water swellability, existing Related Cases report that it easily blocks after being eaten by mistake
Enteron aisle.Therefore, the polyacrylamide gel matrix and high hydroscopic resin gel-type vehicle are unsuitable for, for potted plant, using model
Enclose narrow.
The content of the invention
In view of this, the present invention provides a kind of agarose gel matrix and preparation method thereof, and the agarose gel matrix is not
Spill, user can watch the root system of cultivated plant by matrix, water the cycle flexibly, without often addition or replacing nutrition
Composition, improves Consumer's Experience, and it is repeatable utilize, it is nontoxic, using scope is wide, low cost.
The present invention is solved the above problems by following technological means:
A kind of preparation method of agarose gel matrix of the invention, including:According to certain mass ratio and volume ratio
Example mixes the inorganic salts of water, the agar powder of the first preset concentration and the second preset concentration, obtains the first mixture;By described
One mixture is heated and stirred, until the first mixture boiled and agar powder and inorganic salts are completely dissolved, obtains
One solution;The pH value of first solution is adjusted to the first default pH value, and first solution adjusted after pH value is poured into
In container, solid gel shape is cooled at room temperature after removing bubble, obtain the first nutritive layer;According to certain mass ratio and body
Product ratio is mixed the agar powder of water and the 3rd preset concentration, obtains the second mixture;By second mixture heating
And stir, until the second mixture boiled and agar powder is completely dissolved, obtains the second solution;Adjust described second molten
The pH value of liquid is fallen to the second default pH value, and after second solution adjusted after pH value is cooled into preset temperature at room temperature
On first nutritive layer, solid gel shape is continued cool at room temperature after removing bubble, obtain the second nutritive layer.
Further, first preset concentration is 0.8g/100mL~2g/100mL.
Further, the value of second preset concentration is 5~10 times of appropriate inorganic salinity.
Further, the 3rd preset concentration is 0.5g/100mL~1g/100mL.
Further, the preset temperature is 65 DEG C~80 DEG C.
Further, the inorganic salts are inorganic salt powder or inorganic salts concentrate.
Further, before pH value to the first default pH value of regulation first solution, also include:Described
The first pigment is added in one solution;Before pH value to the second default pH value of regulation second solution, also include:
The second pigment is added in second solution.
Further, the scope of the described first default pH value is 5~8, and the scope of the described second default pH value is 5~8.
A kind of agarose gel matrix of the invention, including:Water, the agar powder of the first preset concentration, the second preset concentration
Inorganic salts and the 3rd preset concentration agar powder.
Further, first preset concentration is 0.8g/100mL~2g/100mL, and the value of second preset concentration is 5
~10 times of appropriate inorganic salinity, the 3rd preset concentration is 0.5g/100mL~1g/100mL.
A kind of agarose gel matrix of the invention and preparation method thereof has the advantages that:
1. agarose gel matrix of the invention is solid, is not spilt;
2. agarose gel matrix of the invention is translucent gels, is put into transparent vessel during for soilless culture, user
The root system of cultivated plant can be watched by matrix, Consumer's Experience is improve;
3. agarose gel matrix of the invention includes the first nutritive layer and the second nutritive layer, and user can select according to demand
First nutritive layer and the second nutritive layer of certain volume, this double-deck agarose gel matrix can containing it is substantial amounts of, in ladder
The described inorganic salts of the cultivated plant growth of diffusion profile are spent, without often supplementing the nutrients, cultivated plant will not be also caused
Dehydration is caused to damage by the inorganic salts of high concentration;
4. pigment, the container of selection different shaping can be also added in agarose gel matrix of the invention, different face are obtained
Color, the agarose gel matrix of moulding, view and admire for user;
5. agarose gel matrix of the invention has good retentiveness, is absorbed water after dehydration restorative, so that pouring
The water cycle is flexible;
6. agarose gel matrix of the invention can be after heating and melting, then cooled and solidified, repeats and utilizes;Agarose
It is the natural polysaecharides material extracted from algae, used as conventional food additives, nontoxic, low cost uses scope
Extensively;
7. agarose gel matrix of the invention has elasticity and toughness, it may not be necessary to the container such as vase or flowerpot, directly
Connect and provide physical support, easy to use and low cost to cultivated plant;
8. agarose gel matrix of the invention can be prepared using running water, and operating environment and operative material are without aseptic
Treatment;
9. agarose gel matrix of the invention need not add antibiotic, bacteriostatic agent, and itself can prevent living contaminants.
In sum, agarose gel matrix of the invention does not spill, and user can watch cultivated plant by matrix
Root system, water the cycle flexibly, without often addition or change nutritional ingredient, improve Consumer's Experience, and it is repeatable utilize, nothing
Poison, using scope is wide, low cost.
Brief description of the drawings
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is a kind of schematic flow sheet of the preparation method of agarose gel matrix of the invention.
Specific embodiment
Below with reference to accompanying drawing, the present invention is described in detail, as shown in Figure 1:A kind of Ago-Gel of the present embodiment
The preparation method of matrix comprises the following steps:
101st, it is according to certain mass ratio and volume ratio that water, the agar powder of the first preset concentration and second is default dense
The inorganic salts mixing of degree, obtains the first mixture.
Wherein, first preset concentration is 0.8g/100mL~2g/100mL, the value of second preset concentration for 5~
10 times of appropriate inorganic salinity, the inorganic salts are inorganic salt powder or inorganic salts concentrate, and water can be running water.
It should be noted that the species of the inorganic salts, composition and suitable concentration depend on the floristics to be cultivated.
102nd, first mixture is heated and is stirred, until first mixture boiled and the agar powder and nothing
Machine salt is completely dissolved, and obtains the first solution.
Wherein, the scope of the pH value of the first solution is 5~8.
103rd, the first pigment is added in first solution.
104th, the pH value of first solution is adjusted to the first default pH value, and will adjust first solution after pH value
Pour into container, solid gel shape is cooled at room temperature after removing bubble, obtain the first nutritive layer.
Wherein, the scope of the described first default pH value is 5~8.
105th, the agar powder of water and the 3rd preset concentration is mixed according to certain mass ratio and volume ratio, is obtained
Second mixture.
Wherein, the 3rd preset concentration is 0.5g/100mL~1g/100mL.
106th, second mixture is heated and is stirred, until the second mixture boiled and agar powder is complete
Dissolving, obtains the second solution.
Wherein, the scope of the pH value of the second solution is 5~8.
107th, the second pigment is added in second solution.
108th, the pH value of second solution is adjusted to the second default pH value, and will adjust second solution after pH value
It is poured on first nutritive layer after being cooled to preset temperature at room temperature, solid is continued cool at room temperature after removing bubble
Gel, obtains the second nutritive layer.
Wherein, the preset temperature is 65 DEG C~80 DEG C, and the scope of the described second default pH value is 5~8.
It should be noted that user can according to demand select first nutritive layer and the second nutritive layer of different volumes, preferably
, the volume of the second nutritive layer is 5~10 times of the volume of the first nutritive layer.
A kind of agarose gel matrix of the present embodiment, including:It is water, the agar powder of the first preset concentration, second default dense
The agar powder of the inorganic salts of degree and the 3rd preset concentration.
Wherein, first preset concentration is 0.8g/100mL~2g/100mL, the value of second preset concentration for 5~
10 times of appropriate inorganic salinity, the 3rd preset concentration is 0.5g/100mL~1g/100mL.
Specifically, being using the method that the agarose gel matrix carries out plant culture:With glass bar by the second nutritive layer
At stamp behind 1cm~3cm deep space, by vegetable seeds to be cultivated, or clean up and grow macroscopic water root
Plant shoots, or by certain density thick root-promoting hormone treat and the macroscopic water root of strong point cuttage branch
Insert in the space of the second nutritive layer, complete plant culture.
It should be noted that user can according to demand select the container of different shaping freely to mould agarose gel matrix
Moulding.
The present invention provides a kind of agarose gel matrix and preparation method thereof, and its agarose gel matrix for obtaining is solid
Body, does not spill;It is translucent gels, is put into transparent vessel during for soilless culture, user can watches cultivation by matrix
The root system of plant, improves Consumer's Experience;And the agarose gel matrix includes the first nutritive layer and the second nutritive layer, Yong Huke
Selecting certain thickness first nutritive layer and the second nutritive layer, this double-deck agarose gel matrix according to demand can contain
The described inorganic salts of substantial amounts of, diffusion profile in gradient cultivated plant growth, without often supplementing the nutrients, will not also make
Obtain cultivated plant causes dehydration to damage by the inorganic salts of high concentration;It can in addition contain can also add color in agarose gel matrix
The container of element, selection different shaping, obtains the agarose gel matrix of different colours, different shaping, is viewed and admired for user;With very
Good retentiveness, absorbs water restorative, after dehydration so that the cycle of watering is flexible;Can be after heating and melting, then cooled and solidified,
It is repeatable to utilize, it is nontoxic, it is wide using scope;With elasticity and toughness, it may not be necessary to the container such as vase or flowerpot, directly to cultivation
Cultivate thing and physical support, easy to use and low cost are provided;Can be prepared using running water, operating environment and operative material need not
Aseptic process;And antibiotic, bacteriostatic agent need not be added, itself can prevent living contaminants, because comprising only fine jade in gel-type vehicle
This carbon source of lipolysaccharide, it is impossible to absorbed by most microorganisms.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to skill of the invention
Art scheme is modified or equivalent, and without deviating from the objective and scope of technical solution of the present invention, it all should cover at this
In the middle of the right of invention.
Claims (10)
1. a kind of preparation method of agarose gel matrix, it is characterised in that:Including:
According to certain mass ratio and volume ratio by the inorganic of water, the agar powder of the first preset concentration and the second preset concentration
Salt mixes, and obtains the first mixture;
First mixture is heated and stirred, until the first mixture boiled and agar powder and inorganic salts are complete
Dissolving, obtains the first solution;
The pH value of first solution is adjusted to the first default pH value, and first solution adjusted after pH value is poured into container
In, solid gel shape is cooled at room temperature after removing bubble, obtain the first nutritive layer;
The agar powder of water and the 3rd preset concentration is mixed according to certain mass ratio and volume ratio, is obtained the second mixing
Thing;
Second mixture is heated and stirred, until the second mixture boiled and agar powder is completely dissolved, obtains
To the second solution;
The pH value of second solution is adjusted to the second default pH value, and second solution after pH value will be adjusted at room temperature
It is poured on first nutritive layer after being cooled to preset temperature, solid gel shape is continued cool at room temperature after removing bubble,
Obtain the second nutritive layer.
2. the preparation method of a kind of agarose gel matrix according to claim 1, it is characterised in that:Described first presets
Concentration is 0.8g/100mL~2g/100mL.
3. the preparation method of a kind of agarose gel matrix according to claim 2, it is characterised in that:Described second presets
The value of concentration is 5~10 times of appropriate inorganic salinity.
4. the preparation method of a kind of agarose gel matrix according to claim 3, it is characterised in that:Described 3rd presets
Concentration is 0.5g/100mL~1g/100mL.
5. the preparation method of a kind of agarose gel matrix according to claim 4, it is characterised in that:The preset temperature
It is 65 DEG C~80 DEG C.
6. the preparation method of a kind of agarose gel matrix according to claim 1, it is characterised in that:The inorganic salts are
Inorganic salt powder or inorganic salts concentrate.
7. the preparation method of a kind of agarose gel matrix according to claim any one of 1-6, it is characterised in that:Institute
Before stating pH value to the first default pH value for adjusting first solution, also include:
The first pigment is added in first solution;
Before pH value to the second default pH value of regulation second solution, also include:
The second pigment is added in second solution.
8. the preparation method of a kind of agarose gel matrix according to claim 1, it is characterised in that:Described first presets
The scope of pH value is 5~8, and the scope of the described second default pH value is 5~8.
9. a kind of agarose gel matrix, it is characterised in that:Including:Water, the agar powder of the first preset concentration, the second preset concentration
Inorganic salts and the 3rd preset concentration agar powder.
10. a kind of agarose gel matrix according to claim 9, it is characterised in that:First preset concentration is
0.8g/100mL~2g/100mL, the value of second preset concentration is 5~10 times of appropriate inorganic salinity, and the described 3rd is pre-
If concentration is 0.5g/100mL~1g/100mL.
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