CN111492942A - Gel matrix for plant branch cutting cultivation and production line thereof - Google Patents

Gel matrix for plant branch cutting cultivation and production line thereof Download PDF

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Publication number
CN111492942A
CN111492942A CN202010414290.XA CN202010414290A CN111492942A CN 111492942 A CN111492942 A CN 111492942A CN 202010414290 A CN202010414290 A CN 202010414290A CN 111492942 A CN111492942 A CN 111492942A
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gel
low
agarose
tank
gel layer
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朱家伟
韩超
夏慧
王蒋
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Nanjing Ninglv Biotechnology Co Ltd
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Nanjing Ninglv Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/40Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
    • A01G24/44Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure in block, mat or sheet form
    • A01G24/46Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure in block, mat or sheet form multi-layered
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/30Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00Processes of treating or compounding macromolecular substances
    • C08J3/02Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
    • C08J3/03Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
    • C08J3/075Macromolecular gels
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/12Agar-agar; Derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a gel matrix for cuttage cultivation of plant branches and a production line thereof, wherein the gel matrix is formed by laminating a plurality of functional gel layers and comprises a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and an antibacterial gel layer, and the application of gel in the plant cuttage direction is realized by compounding a plurality of functional gels, so that the rooting of cuttage plants can be effectively promoted, plant individuals can be quickly cultivated and are antibacterial, and the survival rate of the cuttage plants is ensured.

Description

Gel matrix for plant branch cutting cultivation and production line thereof
Technical Field
The invention relates to the technical field of plant planting, in particular to a gel matrix for cutting cultivation of plant branches and a production line thereof.
Background
Plant cutting, also known as plant cuttings, is a common propagation method for growing plants. The plant is cut into the stem, leaf, root, bud, etc. and inserted into soil, sand or soaked in water to root and be planted separately to become new independent plant.
The conventional plant cuttage needs to use culture media such as soil, sand or water, in the culture process, nutrient substances and water need to be supplemented at any moment, the operation is very complicated, the rooting rate is poor, and the survival rate of the plants is low.
Disclosure of Invention
The invention aims at the problems and discloses a gel matrix for plant branch cutting cultivation and a production line thereof.
The specific technical scheme is as follows:
a gel matrix for cuttage cultivation of plant branches is characterized by being formed by stacking a plurality of functional gel layers, wherein each functional gel layer comprises a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, and the high-concentration nutrient gel layer, the low-concentration nutrient gel layer and the bacteriostatic gel layer are sequentially stacked and superposed from bottom to top;
the high-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, agarose and nutrient substances, the low-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, plant root growth-promoting hormone, agarose and nutrient substances, and the bacteriostatic gel layer is prepared from water, agarose and a bactericide.
The gel matrix for plant branch cutting cultivation is prepared by the following steps:
the preparation method of the high-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, finally cooling at normal temperature, and solidifying to obtain a high-concentration nutrient gel layer;
the preparation method of the low-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, then cooling to 43-45 ℃ at constant temperature, adding plant root growth-promoting hormone, uniformly stirring, finally cooling at normal temperature, and solidifying to obtain a low-concentration nutrient gel layer;
the preparation method of the bacteriostatic gel layer comprises the following steps: adding agarose into water, heating to boil until the agarose is completely dissolved, then adding a bactericide, uniformly stirring, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, finally cooling at normal temperature, and solidifying to obtain the high-concentration nutrient gel layer.
The gel matrix for the cuttage cultivation of the plant branches is characterized in that each liter of high-concentration nutrient gel layer contains 8-10 g of agarose, 0.1g of dehydroethylenediamine, 1-2 g of nutrient substances and the balance of water; 8-10 g of agarose, 0.1g of dehydroethylenediamine, 0.1-0.5 g of nutrient substances, 0.2g of plant root growth-promoting hormone and the balance of water are contained in each liter of low-concentration nutrient gel layer; each liter of the bacteriostatic gel layer contains 10-12 g of agarose, 0.5-1 g of bactericide and the balance of water.
The gel matrix for the cutting cultivation of the plant branches is characterized in that the molar concentration of the hydrogen chloride and the sodium hydroxide is 1 mol/L.
The gel matrix for the cuttage cultivation of the plant branches is characterized in that the nutrient substances are a mixture of a plurality of urea, sodium nitrate, potassium nitrate, calcium superphosphate, ammonium sulfate, potassium sulfate and magnesium sulfate; the plant growth-promoting root hormone is indoleacetic acid or sodium naphthaleneacetate or a mixture of the indoleacetic acid and the sodium naphthaleneacetate; the bactericide is chlorothalonil powder.
A gel matrix production line for plant branch cuttage cultivation is characterized by comprising a first gel complete set, a second gel complete set and a third gel complete set which are respectively used for preparing and canning a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, a conveying belt used for conveying filling bottles and an aluminum foil sealing machine used for sealing the filling bottles;
the first gel complete equipment comprises a first mixing tank, a first heat preservation adjusting tank, a first low-temperature intermediate tank and a first filling machine, wherein the first mixing tank, the first heat preservation adjusting tank, the first low-temperature intermediate tank and the first filling machine are sequentially connected, a first quantitative controller is arranged at a feed inlet of the first mixing tank and used for controlling the supply amount of water, dehydroethylene diamine, agarose and nutrient substances, and the first heat preservation adjusting tank is communicated with the first mixing tank and provided with a second quantitative controller used for controlling the supply amount of hydrogen chloride and sodium hydroxide;
the second gel complete equipment comprises a second mixing tank, a second heat-preservation adjusting tank, a second low-temperature intermediate tank and a second filling machine, wherein the second mixing tank, the second heat-preservation adjusting tank, the second low-temperature intermediate tank and the second filling machine are sequentially connected, a third quantitative controller is arranged at a feed inlet of the second mixing tank and used for controlling the supply amount of water, dehydroethylenediamine, agarose and nutrient substances, the second heat-preservation adjusting tank is communicated with the second mixing tank and provided with a fourth quantitative controller used for controlling the supply amount of hydrogen chloride and sodium hydroxide, and the second low-temperature intermediate tank is communicated with the second heat-preservation adjusting tank and provided with a fifth quantitative controller used for controlling the supply amount of plant growth-promoting root hormone;
the third gel complete equipment comprises a third material mixing tank, a third heat-preservation adjusting tank, a third low-temperature intermediate tank and a third filling machine, wherein the third material mixing tank, the third heat-preservation adjusting tank, the third low-temperature intermediate tank and the third filling machine are sequentially connected, a sixth quantitative controller is arranged at a material inlet of the third material mixing tank and used for controlling the supply amount of water and agarose, and the third heat-preservation adjusting tank is communicated with the third material mixing tank and provided with a seventh quantitative controller and used for controlling the supply amount of bactericide;
the conveying belt is arranged along the directions of the first gel complete equipment, the second gel complete equipment, the third gel complete equipment and the aluminum foil sealing machine, is positioned below the first filling machine, the second filling machine and the third filling machine, and is communicated with the aluminum foil sealing machine.
The production process of the gel matrix production line for cutting cultivation of the plant branches comprises the following specific steps:
(1) preparing functional gel;
(1-1) preparation of high-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a first mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, stirring uniformly, continuing for 10 minutes to obtain a fluid high-concentration nutrient gel, then pumping into a first heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a first low-temperature intermediate tank after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(1-2) preparing a low-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a second mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, stirring uniformly, continuing for 10 minutes to obtain a fluid low-concentration nutrient gel, then pumping into a second heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; after the PH value is adjusted, cooling to 43-45 ℃, adding plant root growth promoting hormone, uniformly stirring, finally pumping into a second low-temperature intermediate tank, and preserving heat and temporarily storing at 35-40 ℃;
(1-3) preparing an antibacterial gel: quantitatively adding water and agarose into a third mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding a bactericide, uniformly stirring, continuing for 10 minutes to obtain a fluid antibacterial gel, then pumping into a third heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a third low-temperature intermediate tank after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(2) layering and canning the gel; the method comprises the following steps that filling bottles sequentially pass through a first filling machine, a second filling machine and a third filling machine through a conveyor belt, temporarily-stored fluid high-concentration nutrient gel is guided into the first filling machine and is filled at the temperature of 32-35 ℃, the high-concentration nutrient gel is filled into the bottom layer of the filling bottle, then, the filling bottle which completes filling at the bottom layer is moved to the second filling machine, the temporarily-stored fluid low-concentration nutrient gel is guided into the second filling machine and is filled at the temperature of 32-35 ℃, the low-concentration nutrient gel is filled into the middle layer of the filling bottle, finally, the filling bottle which completes filling at the middle layer is moved to the third filling machine, the temporarily-stored fluid bacteriostatic gel is guided into the third filling machine and is filled at the temperature of 32-35 ℃, and the bacteriostatic gel is filled into;
(3) and (4) sealing and capping the filling bottle: and conveying the canned filling bottles to an aluminum foil sealing machine through a conveyor belt to finish aluminum foil sealing, and covering to obtain a canned gel matrix finished product.
The gel matrix production line for plant branch cuttage cultivation is characterized in that a turnover table is arranged on the conveyor belt, the canning middle time is adjusted, the canning time of two adjacent times is controlled to be 10-20 minutes, and the coagulation of the canned functional gel is guaranteed.
The gel matrix production line for plant branch cuttage cultivation is characterized in that the volume ratio of the high-concentration nutrient gel layer to the low-concentration nutrient gel layer to the bacteriostatic gel layer in the canned gel matrix finished product is 1: 10-20: 0.5-5.
The above gel matrix production line for plant branch cuttage cultivation, wherein, the aluminium foil capper is full-automatic aluminium foil capper.
The invention has the beneficial effects that:
the invention discloses a gel matrix for cuttage cultivation of plant branches and a production line thereof, wherein the gel matrix is formed by laminating a plurality of functional gel layers and comprises a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and an antibacterial gel layer, and the application of gel in the plant cuttage direction is realized by compounding a plurality of functional gels, so that the rooting of cuttage plants can be effectively promoted, plant individuals can be quickly cultivated and are antibacterial, and the survival rate of the cuttage plants is ensured.
Drawings
FIG. 1 is a schematic view of the present invention.
FIG. 2 is a schematic diagram of a gel matrix production process.
FIG. 3 is a schematic view of a gel matrix production line.
The reference numbers illustrate: 1. a first gel kit; 2. a second gel kit; 3. a third gel kit; 4. a first mixing tank; 5. a first heat-preserving conditioning tank; 6. a first cryogenic intermediate tank; 7. a first filling machine; 8. a second mixing tank; 9. a second heat-preservation adjusting tank; 10. a second cryogenic intermediate tank; 11. a second filling machine; 12. a third mixing tank; 13. a third heat-preservation adjusting tank; 14. a third cryogenic intermediate tank; 15. a third filling machine; 16. a first quantitative controller; 17. a second quantitative controller; 18. a third quantitative controller; 19. a fourth quantitative controller; 20. a fifth quantitative controller; 21. a sixth quantitative controller; 22. a seventh quantitative controller; 23. a conveyor belt; 24. an aluminum foil sealing machine; 25. a turnover table.
Detailed Description
In order to make the technical solution of the present invention clearer and clearer, the present invention is further described below with reference to embodiments, and any solution obtained by substituting technical features of the technical solution of the present invention with equivalents and performing conventional reasoning falls within the scope of the present invention.
Example one
The gel matrix for plant branch cuttage cultivation is characterized by being formed by stacking a plurality of functional gel layers, wherein each functional gel layer comprises a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, and the high-concentration nutrient gel layer, the low-concentration nutrient gel layer and the bacteriostatic gel layer are stacked and superposed from bottom to top in sequence;
the high-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, agarose and nutrient substances, the low-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, plant root growth-promoting hormone, agarose and nutrient substances, and the bacteriostatic gel layer is prepared from water, agarose and a bactericide;
the preparation method of the gel matrix comprises the following steps:
the preparation method of the high-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, finally cooling at normal temperature, and solidifying to obtain a high-concentration nutrient gel layer;
the preparation method of the low-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, then cooling to 43-45 ℃ at constant temperature, adding plant root growth-promoting hormone, uniformly stirring, finally cooling at normal temperature, and solidifying to obtain a low-concentration nutrient gel layer;
the preparation method of the bacteriostatic gel layer comprises the following steps: adding agarose into water, heating to boil until the agarose is completely dissolved, then adding a bactericide, uniformly stirring, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, and finally cooling at normal temperature and solidifying to obtain a high-concentration nutrient gel layer;
the nutrient gel layer comprises 8-10 g of agarose, 0.1g of dehydroethylenediamine, 1-2 g of nutrient substances and the balance of water, and the low-concentration nutrient gel layer comprises 8-10 g of agarose, 0.1g of dehydroethylenediamine, 0.1-0.5 g of nutrient substances, 0.2g of plant root growth-promoting hormone and the balance of water, wherein the bacteriostatic gel layer comprises 10-12 g of agarose, 0.5-1 g of bactericide and the balance of water, the molar concentration of hydrogen chloride and sodium hydroxide is 1 mol/L, the nutrient substances are a mixture of urea, sodium nitrate, potassium nitrate, calcium superphosphate, ammonium sulfate, potassium sulfate and magnesium sulfate, the plant root growth-promoting hormone is indoleacetic acid or sodium naphthylate or a mixture of indoleacetic acid and sodium naphthylacetate;
according to the embodiment, the multifunctional expansion of the gel is realized by improving the gel formula, the application of the gel in the plant cuttage direction is realized by compounding a plurality of functional gels, the plant cuttage is carried out through the gel matrix of the embodiment, the plant rooting rate and the survival rate can be effectively ensured and improved, additional supplement of nutrient substances and water is not needed, and the use is convenient;
the gel matrix of the embodiment is compounded by adopting a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, wherein the low-concentration nutrient gel layer is rich in plant root growth promoting hormone and promotes the rooting of the cuttage plants, the high-concentration nutrient gel layer can be absorbed by the rooted cuttage plants to quickly culture individual plants, and the bacteriostatic gel layer can effectively inhibit bacteria, prevent the root system infection of the cuttage plants and ensure the survival rate of the cuttage plants;
the selection of nutrients can be adjusted according to the nutritional needs of the plant species.
Example two
The gel matrix production line for plant branch cuttage cultivation is characterized by comprising a first gel complete equipment 1, a second gel complete equipment 2 and a third gel complete equipment 3 which are respectively used for preparing and canning a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, a conveyor belt 23 used for conveying filling bottles and an aluminum foil sealing machine 24 used for sealing the filling bottles;
the first gel complete equipment 1 comprises a first mixing tank 4, a first heat-preservation adjusting tank 5, a first low-temperature intermediate tank 6 and a first filling machine 7, wherein the first mixing tank 4, the first heat-preservation adjusting tank 5, the first low-temperature intermediate tank 6 and the first filling machine 7 are sequentially connected, a first quantitative controller 16 is arranged at a feed inlet of the first mixing tank 4 and used for controlling the supply amount of water, dehydroethylenediamine, agarose and nutrient substances, and the first heat-preservation adjusting tank 5 is communicated with the first mixing tank 4 and provided with a second quantitative controller 17 used for controlling the supply amount of hydrogen chloride and sodium hydroxide;
the second gel complete equipment 2 comprises a second mixing tank 8, a second heat-preservation adjusting tank 9, a second low-temperature intermediate tank 10 and a second filling machine 11, wherein the second mixing tank 8, the second heat-preservation adjusting tank 9, the second low-temperature intermediate tank 10 and the second filling machine 11 are sequentially connected, a third quantitative controller 18 is arranged at a feed inlet of the second mixing tank 8 and used for controlling the supply amount of water, dehydroethylenediamine, agarose and nutrient substances, the second heat-preservation adjusting tank 9 is communicated with the second mixing tank 8 and provided with a fourth quantitative controller 19 for controlling the supply amount of hydrogen chloride and sodium hydroxide, and the second low-temperature intermediate tank 10 is communicated with the second heat-preservation adjusting tank 9 and provided with a fifth quantitative controller 20 for controlling the supply amount of plant growth-promoting root hormone;
the third gel complete equipment 3 comprises a third mixing tank 12, a third heat-preservation adjusting tank 13, a third low-temperature intermediate tank 14 and a third filling machine 15, wherein the third mixing tank 12, the third heat-preservation adjusting tank 13, the third low-temperature intermediate tank 14 and the third filling machine 15 are sequentially connected, a sixth quantitative controller 21 is arranged at a feed inlet of the third mixing tank 12 and used for controlling the supply of water and agarose, and the third heat-preservation adjusting tank 13 is communicated with the third mixing tank 12 and provided with a seventh quantitative controller 22 used for controlling the supply of bactericide;
the conveyor belt 23 is arranged along the directions of the first gel complete equipment 1, the second gel complete equipment 2, the third gel complete equipment 3 and the aluminum foil sealing machine 24, is positioned below the first filling machine 7, the second filling machine 11 and the third filling machine 15, and is communicated with the aluminum foil sealing machine 24;
the production process of the gel covering matrix production line comprises the following specific steps:
(1) preparing functional gel;
(1-1) preparation of high-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a first mixing tank 4, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, uniformly stirring, continuing for 10 minutes to obtain a fluid high-concentration nutrient gel, then pumping into a first heat preservation adjusting tank 5, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a first low-temperature intermediate tank 6 after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(1-2) preparing a low-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a second mixing tank 8, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, uniformly stirring, continuing for 10 minutes to obtain a fluid low-concentration nutrient gel, then pumping into a second heat preservation adjusting tank 9, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; after the PH value is adjusted, cooling to 43-45 ℃, adding plant root growth promoting hormone, uniformly stirring, finally pumping into a second low-temperature intermediate tank 10, and preserving heat and temporarily storing at 35-40 ℃;
(1-3) preparing an antibacterial gel: quantitatively adding water and agarose into a third mixing tank 12, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding a bactericide, uniformly stirring, continuing for 10 minutes to obtain a fluid-shaped antibacterial gel, then pumping into a third heat preservation adjusting tank 13, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a third low-temperature intermediate tank 14 after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(2) layering and canning the gel; the filling bottles sequentially pass through a first filling machine 7, a second filling machine 11 and a third filling machine 15 through a conveyor belt 23, temporarily stored fluid high-concentration nutrient gel is guided into the first filling machine 7, canning is carried out at the temperature of 32-35 ℃, the high-concentration nutrient gel is filled into the bottom layer of the filling bottle, then the filling bottle which completes the canning of the bottom layer moves to the second filling machine 11, the temporarily stored fluid low-concentration nutrient gel is guided into the second filling machine 11, canning is carried out at the temperature of 32-35 ℃, the low-concentration nutrient gel is filled into the middle layer of the filling bottle, finally, the filling bottle which completes the canning of the middle layer moves to the third filling machine 15, the temporarily stored fluid bacteriostatic gel is guided into the third filling machine 15, canning is carried out at the temperature of 32-35 ℃, and the bacteriostatic gel is filled into the top layer of;
(3) and (4) sealing and capping the filling bottle: conveying the canned filling bottles to an aluminum foil sealing machine 24 through a conveyor belt 23 to complete aluminum foil sealing, and covering to obtain canned gel matrix finished products;
the conveying belt 23 is provided with a turnover table 25, the canning middle time is adjusted, the canning time of two adjacent times is controlled to be 10-20 minutes, and the coagulation of the canned functional gel is ensured; the volume ratio of the high-concentration nutrient gel layer to the low-concentration nutrient gel layer to the bacteriostatic gel layer in the canned gel matrix finished product is 1: 10-20: 0.5-5; the aluminum foil sealing machine 24 is a full-automatic aluminum foil sealing machine 24;
the embodiment discloses a production line of a gel matrix and a production process thereof, the canned gel matrix can be rapidly prepared by the production line and the process, the production is stable, the efficiency is high, the prepared gel matrix can be directly used for cuttage of cuttage plants, and the use is convenient and fast.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. A gel matrix for cuttage cultivation of plant branches is characterized by being formed by stacking a plurality of functional gel layers, wherein each functional gel layer comprises a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, and the high-concentration nutrient gel layer, the low-concentration nutrient gel layer and the bacteriostatic gel layer are sequentially stacked and superposed from bottom to top;
the high-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, agarose and nutrient substances, the low-concentration nutrient gel layer is prepared from water, dehydroethylenediamine, plant root growth-promoting hormone, agarose and nutrient substances, and the bacteriostatic gel layer is prepared from water, agarose and a bactericide.
2. The gel matrix according to claim 1, wherein the gel matrix is prepared by the following steps:
the preparation method of the high-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, finally cooling at normal temperature, and solidifying to obtain a high-concentration nutrient gel layer;
the preparation method of the low-concentration nutrient gel layer comprises the following steps: adding agarose and nutrient substances into water, heating to boil, uniformly stirring until the agarose is completely dissolved, adding dehydroethylenediamine, uniformly mixing, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, then cooling to 43-45 ℃ at constant temperature, adding plant root growth-promoting hormone, uniformly stirring, finally cooling at normal temperature, and solidifying to obtain a low-concentration nutrient gel layer;
the preparation method of the bacteriostatic gel layer comprises the following steps: adding agarose into water, heating to boil until the agarose is completely dissolved, then adding a bactericide, uniformly stirring, adjusting the pH value to 6.5-7.5 by using hydrogen chloride and sodium hydroxide, finally cooling at normal temperature, and solidifying to obtain the high-concentration nutrient gel layer.
3. The gel matrix for plant branch cutting cultivation according to claim 2, wherein each liter of the high-concentration nutrient gel layer contains 8-10 g of agarose, 0.1g of dehydroethylenediamine, 1-2 g of nutrients, and the balance of water; 8-10 g of agarose, 0.1g of dehydroethylenediamine, 0.1-0.5 g of nutrient substances, 0.2g of plant root growth-promoting hormone and the balance of water are contained in each liter of low-concentration nutrient gel layer; each liter of the bacteriostatic gel layer contains 10-12 g of agarose, 0.5-1 g of bactericide and the balance of water.
4. The gel matrix for use in plant shoot cutting cultivation according to claim 3, wherein the molar concentration of said hydrogen chloride and sodium hydroxide is 1 mol/L.
5. The gel matrix according to claim 4, wherein the nutrient is a mixture of urea, sodium nitrate, potassium nitrate, calcium superphosphate, ammonium sulfate, potassium sulfate, magnesium sulfate; the plant growth-promoting root hormone is indoleacetic acid or sodium naphthaleneacetate or a mixture of the indoleacetic acid and the sodium naphthaleneacetate; the bactericide is chlorothalonil powder.
6. A gel matrix production line for plant branch cuttage cultivation is characterized by comprising a first gel complete set, a second gel complete set and a third gel complete set which are respectively used for preparing and canning a high-concentration nutrient gel layer, a low-concentration nutrient gel layer and a bacteriostatic gel layer, a conveying belt used for conveying filling bottles and an aluminum foil sealing machine used for sealing the filling bottles;
the first gel complete equipment comprises a first mixing tank, a first heat preservation adjusting tank, a first low-temperature intermediate tank and a first filling machine, wherein the first mixing tank, the first heat preservation adjusting tank, the first low-temperature intermediate tank and the first filling machine are sequentially connected, a first quantitative controller is arranged at a feed inlet of the first mixing tank and used for controlling the supply amount of water, dehydroethylene diamine, agarose and nutrient substances, and the first heat preservation adjusting tank is communicated with the first mixing tank and provided with a second quantitative controller used for controlling the supply amount of hydrogen chloride and sodium hydroxide;
the second gel complete equipment comprises a second mixing tank, a second heat-preservation adjusting tank, a second low-temperature intermediate tank and a second filling machine, wherein the second mixing tank, the second heat-preservation adjusting tank, the second low-temperature intermediate tank and the second filling machine are sequentially connected, a third quantitative controller is arranged at a feed inlet of the second mixing tank and used for controlling the supply amount of water, dehydroethylenediamine, agarose and nutrient substances, the second heat-preservation adjusting tank is communicated with the second mixing tank and provided with a fourth quantitative controller used for controlling the supply amount of hydrogen chloride and sodium hydroxide, and the second low-temperature intermediate tank is communicated with the second heat-preservation adjusting tank and provided with a fifth quantitative controller used for controlling the supply amount of plant growth-promoting root hormone;
the third gel complete equipment comprises a third material mixing tank, a third heat-preservation adjusting tank, a third low-temperature intermediate tank and a third filling machine, wherein the third material mixing tank, the third heat-preservation adjusting tank, the third low-temperature intermediate tank and the third filling machine are sequentially connected, a sixth quantitative controller is arranged at a material inlet of the third material mixing tank and used for controlling the supply amount of water and agarose, and the third heat-preservation adjusting tank is communicated with the third material mixing tank and provided with a seventh quantitative controller and used for controlling the supply amount of bactericide;
the conveying belt is arranged along the directions of the first gel complete equipment, the second gel complete equipment, the third gel complete equipment and the aluminum foil sealing machine, is positioned below the first filling machine, the second filling machine and the third filling machine, and is communicated with the aluminum foil sealing machine.
7. The gel matrix production line for plant shoot cutting cultivation according to claim 6, wherein the production process of the gel matrix production line comprises the following specific steps:
(1) preparing functional gel;
(1-1) preparation of high-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a first mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, stirring uniformly, continuing for 10 minutes to obtain a fluid high-concentration nutrient gel, then pumping into a first heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a first low-temperature intermediate tank after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(1-2) preparing a low-concentration nutrient gel: quantitatively adding water, agarose and nutrient substances into a second mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding dehydroethylenediamine, stirring uniformly, continuing for 10 minutes to obtain a fluid low-concentration nutrient gel, then pumping into a second heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; after the PH value is adjusted, cooling to 43-45 ℃, adding plant root growth promoting hormone, uniformly stirring, finally pumping into a second low-temperature intermediate tank, and preserving heat and temporarily storing at 35-40 ℃;
(1-3) preparing an antibacterial gel: quantitatively adding water and agarose into a third mixing tank, heating to boil, preserving heat, stirring at a high speed, controlling the stirring speed at 1500-2000R/min, continuing for 20-30 minutes, adding a bactericide, uniformly stirring, continuing for 10 minutes to obtain a fluid antibacterial gel, then pumping into a third heat preservation adjusting tank, preserving heat at 65-75 ℃, and adding hydrogen chloride and sodium hydroxide to adjust the pH value to 6.5-7.5; pumping into a third low-temperature intermediate tank after the pH value is adjusted, and preserving heat and temporarily storing at 35-40 ℃;
(2) layering and canning the gel; the method comprises the following steps that filling bottles sequentially pass through a first filling machine, a second filling machine and a third filling machine through a conveyor belt, temporarily-stored fluid high-concentration nutrient gel is guided into the first filling machine and is filled at the temperature of 32-35 ℃, the high-concentration nutrient gel is filled into the bottom layer of the filling bottle, then, the filling bottle which completes filling at the bottom layer is moved to the second filling machine, the temporarily-stored fluid low-concentration nutrient gel is guided into the second filling machine and is filled at the temperature of 32-35 ℃, the low-concentration nutrient gel is filled into the middle layer of the filling bottle, finally, the filling bottle which completes filling at the middle layer is moved to the third filling machine, the temporarily-stored fluid bacteriostatic gel is guided into the third filling machine and is filled at the temperature of 32-35 ℃, and the bacteriostatic gel is filled into;
(3) and (4) sealing and capping the filling bottle: and conveying the canned filling bottles to an aluminum foil sealing machine through a conveyor belt to finish aluminum foil sealing, and covering to obtain a canned gel matrix finished product.
8. The gel matrix production line for plant branch cutting cultivation according to claim 7, wherein a revolving table is arranged on the conveyor belt, the middle time of canning is adjusted, and the canning time of two adjacent times is controlled within 10-20 minutes, so that the coagulation of the canned functional gel is ensured.
9. The gel matrix production line for plant branch cutting cultivation according to claim 8, wherein the volume ratio of the high-concentration nutrient gel layer, the low-concentration nutrient gel layer and the bacteriostatic gel layer in the canned finished gel matrix is 1: 10-20: 0.5-5.
10. The gel matrix production line for plant shoot cutting cultivation according to claim 9, wherein the aluminum foil sealer is a fully automatic aluminum foil sealer.
CN202010414290.XA 2020-05-15 2020-05-15 Gel matrix for plant branch cutting cultivation and production line thereof Withdrawn CN111492942A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202514487U (en) * 2012-02-28 2012-11-07 中国热带农业科学院橡胶研究所 Production line for yield stimulant of rubber trees
CN105123680A (en) * 2015-08-25 2015-12-09 钱夕华 Low-concentration NAA gel root dipping method
CN106888950A (en) * 2017-02-23 2017-06-27 朱家伟 A kind of agarose gel matrix and preparation method thereof
CN107347473A (en) * 2017-07-04 2017-11-17 岭南园林股份有限公司 A kind of trees drip solution production system and its production technology
CN108617296A (en) * 2018-04-20 2018-10-09 泉州医学高等专科学校 A kind of cottage method of liana plant

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202514487U (en) * 2012-02-28 2012-11-07 中国热带农业科学院橡胶研究所 Production line for yield stimulant of rubber trees
CN105123680A (en) * 2015-08-25 2015-12-09 钱夕华 Low-concentration NAA gel root dipping method
CN106888950A (en) * 2017-02-23 2017-06-27 朱家伟 A kind of agarose gel matrix and preparation method thereof
CN107347473A (en) * 2017-07-04 2017-11-17 岭南园林股份有限公司 A kind of trees drip solution production system and its production technology
CN108617296A (en) * 2018-04-20 2018-10-09 泉州医学高等专科学校 A kind of cottage method of liana plant

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