CN109042215B - Treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings - Google Patents
Treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G23/00—Forestry
- A01G23/02—Transplanting, uprooting, felling or delimbing trees
- A01G23/04—Transplanting trees; Devices for grasping the root ball, e.g. stump forceps; Wrappings or packages for transporting trees
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention discloses a treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings, which comprises the following steps: 1) taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 5-10 seconds; 2) uniformly placing the bottle seedlings obtained in the step 1) on a seedling culture tray filled with a sterilized seedling culture medium according to the specification of the row spacing of 5-10cm, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil with the particle size of 1-3 mm, and covering a sunshade net; 3) after culturing for 2-3 days under a sunshade net, spraying a rooting deep sea fish oil dilution solution on leaves of the eucalyptus tissue culture bottle seedlings; continuously culturing for 3-5 days, and packaging and transporting. By the method, roots of eucalyptus tissue culture bottle seedlings are not easy to rot in the long-distance transportation process, the bottle seedlings are less damaged, and the survival rate is high during transplanting.
Description
Technical Field
The invention relates to the field of seedling transportation, in particular to a treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings.
Technical Field
Eucalyptus globulus Smith (Eucalyptus robusta Smith) is a plant of Myrtaceae (Myrtaceae). Eucalyptus is a fast growing high-yield forest with the largest planting area in south China, the seedling is mainly bred in a tissue culture mode, and tissue culture factories for producing eucalyptus tissue culture bottle seedlings are mainly concentrated in Tuxi county in Guangdong, Nanning City in Guangxi, Susei county, guest city and Liuzhou city in Guangxi. The transplantation of tissue culture bottle seedlings and the seedling management after the transplantation are mainly distributed in nursery gardens suitable for planting eucalyptus in towns and towns, such as Guangxi, Guangdong, Fujian, Yunnan, Guizhou, Sichuan and the like, and qualified seedlings are cultured for forestation. Therefore, the eucalyptus tissue culture bottle seedlings need to be transported to each nursery for transplantation.
In the long-distance transportation of eucalyptus tissue culture bottle seedlings, the bottle seedlings always grow in the culture bottle in the cultivation process, the growth environment is superior, and plants are tender. Once the root culture medium is cleaned, the eucalyptus tissue culture bottle seedlings are placed in a crowded foam transport box, and under the condition that adverse factors such as air non-circulation and vehicle bumping are caused in the transport process, the roots of the eucalyptus tissue culture bottle seedlings are easy to rot, so that the survival rate of the transplanted eucalyptus tissue culture bottle seedlings is low, the seedlings are lost for seedling growers, and meanwhile, the long-distance transport and short-term storage of the eucalyptus tissue culture bottle seedlings are also restricted.
The patent No. 201610149963.7 discloses a treatment method for long-distance transportation resistance of eucalyptus tissue culture seedlings, which comprises the steps of carrying out outdoor water culture for 1-3 times, pouring out auxin in a square plate, supplementing pure tap water for culture for 1-3 days, treating with the auxin, replacing the auxin in the square plate every 4-7 days, and carrying out packing transportation after 15-40 days of water culture. However, the method is complicated, takes a long time and has high production cost.
Disclosure of Invention
In order to solve the technical problems, the invention provides a treatment method for eucalyptus tissue culture bottle seedlings to resist long-distance transportation, so that roots of the eucalyptus tissue culture bottle seedlings are not easy to rot in the process of resisting long-distance transportation, the bottle seedlings are less damaged, the survival rate is high during transplantation, the working procedures are simple, and the production cost is low.
The technical scheme adopted by the invention is that the treatment method for resisting long-distance transportation of the eucalyptus tissue culture bottle seedlings comprises the following steps:
1) taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 5-10 seconds;
2) uniformly and vertically placing the bottle seedlings obtained in the step 1) on a seedling raising tray filled with a sterilized seedling raising substrate according to the specification of the row spacing of 5-10cm, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil with the particle size of 1-3 mm, and covering a sunshade net;
3) after culturing for 2-3 days under a sunshade net, spraying a rooting deep sea fish oil diluent on leaves of the eucalyptus tissue culture bottle seedlings; continuously culturing for 3-5 days, and packaging and transporting.
Further, the yellow mud slurry in the step 1) is prepared by fully and uniformly mixing 5-10 parts by weight of yellow core soil, 0.05-0.1 part by weight of potassium dihydrogen phosphate and 10-20 parts by weight of clear water; then sequentially adding 0.1-0.5 part of hydroxyethyl cellulose, 0.05-0.1 part of potassium polyacrylate and 0.002-0.005 part of carbendazim powder with the effective component content of 50 percent, and fully and uniformly mixing to prepare the bactericide.
Further, the seedling substrate in the step 2) is prepared by mixing 60-70 parts of red heart soil, 10-30 parts of coconut coir, 3-4 parts of hydroxyethyl cellulose, 0.5-1 part of monopotassium phosphate and 0.5-1 part of potassium polyaspartate according to parts by weight.
Further, when placing eucalyptus tissue culture bottle seedlings in the step 2), cleaning each bottle of bottle seedlings with a culture medium, and then placing each bottle of bottle seedlings on a seedling raising tray filled with a sterilized substrate as a bunch without being disassembled into a single plant; the placing line spacing is 5-10 cm.
Further, when the cultivation is continued in the step 3), the leaves and stems of the eucalyptus tissue culture bottle seedlings turn red slightly from green, and new roots grow out, and then the seedlings are packaged and transported.
Further, when the eucalyptus tissue culture bottle seedlings are packaged and transported, the treated eucalyptus tissue culture bottle seedlings are placed in a foam box in a tidy and vertical mode, and then 70% of root rot wettable powder solution diluted by 1000 times is sprayed on leaf surfaces.
The invention has the following beneficial effects:
1. in the formula of the yellow mud and the seedling substrate, potassium dihydrogen phosphate, hydroxyethyl cellulose, potassium polyacrylate and potassium polyaspartate are mainly used as water retention agents, so that eucalyptus tissue culture bottle seedlings are prevented from dying due to water loss, and rapid rooting of the bottle seedlings during culture on a seedling tray is promoted; meanwhile, the monopotassium phosphate can be used for aging the stems and leaves of the eucalyptus tissue culture bottle seedlings and preventing the eucalyptus tissue culture bottle seedlings from being damaged easily in transportation.
2. The yellow core soil has strong viscosity, and is easy to adhere to the root system of the eucalyptus tissue culture bottle seedling after being made into yellow mud; the red heart soil has large porosity and good air permeability, and the root system of the eucalyptus tissue culture bottle seedling is easy to grow new roots.
3. The principle of the whole treatment method in the invention is as follows: the bottle seedlings of the eucalyptus tissue culture are cultured in a short period, so that the bottle seedlings rapidly take roots; and the stems and leaves of the eucalyptus tissue culture bottle seedlings are aged by applying monopotassium phosphate, so that the bottle seedlings are not easily damaged in transportation.
Detailed Description
The invention is further illustrated by the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.
Example 1:
a treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings comprises the following steps:
(1) and taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 10 seconds. The yellow mud is prepared by weighing 10kg of fine yellow core soil, 0.1kg of monopotassium phosphate and 20kg of clear water, and fully and uniformly mixing; then 0.5kg of hydroxyethyl cellulose, 0.1kg of potassium polyacrylate and 5g of carbendazim powder with the effective component content of 50 percent are added in sequence and are fully and evenly mixed to prepare the bactericide.
(2) Uniformly placing eucalyptus tissue culture bottle seedlings soaked in the yellow mud on seedling culture trays filled with seedling culture substrates according to the specification of the row spacing of 10cm, placing 60 bottles (1500 plants) of seedlings on each seedling culture tray, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil with the particle size of 1-3 mm, and covering a sunshade net; the seedling culture substrate is prepared and mixed by the following components in parts by mass: 60kg of red core soil with the particle size of 1-3 mm, 30kg of coconut coir, 3kg of hydroxyethyl cellulose, 1kg of monopotassium phosphate and 1kg of polyaspartic acid potassium.
(3) After 3 days of cultivation under a sunshade net, a Tianyi-rooting deep sea fish oil solution with the dilution volume multiple of 500 times is sprayed on leaves of the eucalyptus tissue culture bottle seedlings.
(4) After continuously culturing for 5 days under a sunshade net, putting the eucalyptus tissue culture bottle seedlings in foam boxes of 50cm (length) multiplied by 30cm (width) multiplied by 10cm (height), placing 5000 eucalyptus tissue culture seedlings in each foam box, and spraying 70% of a 70% rhizofurin wettable powder solution diluted by 1000 times on leaf surfaces.
The growth characters and the long-distance transport resistance effect of the seedlings of the eucalyptus tissue culture bottle seedlings treated by the method are compared and analyzed:
after the eucalyptus tissue culture bottle seedlings are treated by the method, the growth traits of the eucalyptus tissue culture bottle seedlings such as leaves, stems, root hairs and root tips are obviously aging. Before and after the treatment by the method, the growth traits of the eucalyptus tissue culture bottle seedlings are described as the following table 1:
table 1: comparative analysis of growth traits of eucalyptus tissue culture bottle seedlings before and after treatment
| Before treatment in the method | After the treatment of the method | |
| Blade | The leaves are light green, and the leaves are tender | The leaves are reddish brown and obviously aged |
| Stem of straw | The whole stem is light green and is easy to break | The head of the stem is red, has good toughness and is not easy to break |
| Root hair | No new root hair growing | Clearly visible root hair growth |
| Root tip | Elongation of the root tip not clearly seen | Clearly visible root tip elongation |
The method treats 10 thousands of eucalyptus tissue culture bottle seedlings, the labor cost is 200 yuan, the matrix cost is 80 yuan, the water and electricity cost is 20 yuan, the other costs are 50 yuan, and the total cost is 350 yuan. The method treats 10 thousands of eucalyptus tissue culture bottle seedlings, each of which is 0.0035 yuan; in addition, the processing method has short time, and can be packed and transported after being processed for 8 days.
The eucalyptus tissue culture bottle seedlings treated by the method are subjected to long-distance transport resistance effect analysis, and the eucalyptus tissue culture bottle seedlings which are not treated by the method are used as a control. Researches show that the eucalyptus tissue culture bottle seedlings are transported to a nursery garden of Huangtang town in purple county, Heyuan, Guangdong province on a coach through Guangxi Liuzhou city, the transportation distance is about 800km, and the transportation time is 40 hours. After the fertilizer is transported to a nursery, the root rot rate is obviously lower than that of a control after the fertilizer is placed for 3 days, 5 days and 10 days; after the eucalyptus tissue culture seedlings are transported to a nursery garden and placed for 2 days, transplanting is carried out, and the survival rate after 30 days of transplanting is obviously higher than that of a control. Therefore, the eucalyptus tissue culture bottle seedlings subjected to the treatment method have obvious long-distance transportation resistance effect. The specific effect is analyzed by comparison, and is shown in the following table 2:
table 2: comparative analysis on long-distance transport resistance effect of eucalyptus tissue culture bottle seedlings treated by using method
Example 2:
a treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings comprises the following steps:
(1) and taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 5 seconds. The yellow mud is prepared by weighing 5kg of finely-divided yellow core soil, 0.05kg of monopotassium phosphate and 10kg of clear water, and fully and uniformly mixing; then 0.1kg of hydroxyethyl cellulose, 0.05kg of potassium polyacrylate and 2g of carbendazim powder with the effective component content of 50 percent are added in sequence and are fully and evenly mixed to prepare the bactericide.
(2) Uniformly placing eucalyptus tissue culture bottle seedlings soaked in the yellow mud on seedling culture trays filled with seedling culture substrates according to the specification of the row spacing of 5cm, placing 60 bottles (1500 plants) of seedlings on each seedling culture tray, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil with the particle size of 1-3 mm, and covering a sunshade net; the seedling culture substrate is prepared and mixed by the following components in parts by mass: 70kg of red core soil with the particle size of 1-3 mm, 20kg of coconut coir, 4kg of hydroxyethyl cellulose, 0.5kg of monopotassium phosphate and 0.5kg of polyaspartic acid potassium.
(3) After 2 days of cultivation under a sunshade net, a Tianyi-rooting deep sea fish oil solution with the volume multiple of 500 times is sprayed on leaves of the eucalyptus tissue culture bottle seedlings.
(4) After continuously culturing for 3 days under a sunshade net, putting the eucalyptus tissue culture bottle seedlings in foam boxes of 50cm (length) multiplied by 30cm (width) multiplied by 10cm (height), placing 5000 eucalyptus tissue culture seedlings in each foam box, and spraying 70% of a 70% rhizofurin wettable powder solution diluted by 1000 times on leaf surfaces.
The growth characters and the long-distance transport resistance effect of the seedlings of the eucalyptus tissue culture bottle seedlings treated by the method are compared and analyzed:
after the eucalyptus tissue culture bottle seedlings are treated by the method, the growth traits of the eucalyptus tissue culture bottle seedlings such as leaves, stems, root hairs and root tips are obviously aging. Before and after the treatment by the method, the growth traits of the eucalyptus tissue culture bottle seedlings are described as follows:
table 3: comparative analysis of growth traits of eucalyptus tissue culture bottle seedlings before and after treatment
The method treats 8 thousands of eucalyptus tissue culture bottle seedlings, the labor cost is 180 yuan, the matrix cost is 80 yuan, the water and electricity cost is 20 yuan, the other costs are 40 yuan, and the total cost is 320 yuan. The method treats 10 thousands of eucalyptus tissue culture bottle seedlings, each seedling is 0.004 yuan; in addition, the processing method has short time, and the packaging and transportation can be realized after 5 days of processing.
The eucalyptus tissue culture bottle seedlings treated by the method are subjected to long-distance transport resistance effect analysis, and the eucalyptus tissue culture bottle seedlings which are not treated by the method are used as a control. Researches show that the eucalyptus tissue culture bottle seedlings are transported to a certain nursery garden of Yibin city of Sichuan province on a coach bus through Guangxi Liuzhou city, the transportation distance is about 1000km, and the transportation time is 48 hours. After the fertilizer is transported to a nursery, the root rot rate is obviously lower than that of a control after the fertilizer is placed for 3 days, 5 days and 10 days; after the eucalyptus tissue culture seedlings are transported to a nursery garden and placed for 2 days, transplanting is carried out, and the survival rate after 30 days of transplanting is obviously higher than that of a control. Therefore, the eucalyptus tissue culture bottle seedlings subjected to the treatment method have obvious long-distance transportation resistance effect. The specific effect is analyzed by comparison, and is shown in the following table 4:
table 4: comparative analysis on long-distance transport resistance effect of eucalyptus tissue culture bottle seedlings treated by using method
Example 3:
a treatment method for long-distance transportation resistance of eucalyptus tissue culture bottle seedlings comprises the following steps:
(1) and taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 8 seconds. The yellow mud is prepared by weighing 7.5kg of fine yellow core soil, 0.08kg of monopotassium phosphate and 15kg of clear water, and fully and uniformly mixing; then 0.3kg of hydroxyethyl cellulose, 0.08kg of potassium polyacrylate and 4g of carbendazim powder with the effective component content of 50 percent are added in sequence and are fully and evenly mixed to prepare the bactericide.
(2) Uniformly placing eucalyptus tissue culture bottle seedlings soaked in the yellow mud on seedling culture trays filled with seedling culture substrates according to the specification of the row spacing of 5cm, placing 60 bottles (1500 plants) of seedlings on each seedling culture tray, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil with the particle size of 1-3 mm, and covering a sunshade net; the seedling culture substrate is prepared and mixed by the following components in parts by mass: 65kg of red core soil with the particle size of 1-3 mm, 24kg of coconut coir, 3.5kg of hydroxyethyl cellulose, 1kg of monopotassium phosphate and 1kg of polyaspartic acid potassium.
(3) After 3 days of cultivation under a sunshade net, a Tianyi-rooting deep sea fish oil solution with the volume multiple of 500 times is sprayed on leaves of the eucalyptus tissue culture bottle seedlings.
(4) After continuously culturing for 4 days under a sunshade net, putting the eucalyptus tissue culture bottle seedlings in foam boxes of 50cm (length) multiplied by 30cm (width) multiplied by 10cm (height), placing 5000 eucalyptus tissue culture seedlings in each foam box, and spraying 70% of a 70% rhizofurin wettable powder solution of which the leaf surface is 1000 times of that of the eucalyptus tissue culture seedlings.
The growth characters and the long-distance transport resistance effect of the seedlings of the eucalyptus tissue culture bottle seedlings treated by the method are compared and analyzed:
after the eucalyptus tissue culture bottle seedlings are treated by the method, the growth traits of the eucalyptus tissue culture bottle seedlings such as leaves, stems, root hairs and root tips are obviously aging. Before and after the treatment by the method, the growth traits of the eucalyptus tissue culture bottle seedlings are described as follows:
table 5: comparative analysis of growth traits of eucalyptus tissue culture bottle seedlings before and after treatment
| Before treatment in the method | After the treatment of the method | |
| Blade | The leaves are light green, and the leaves are tender | The leaves are reddish brown and obviously aged |
| Stem of straw | The whole stem is light green and is easy to break | The head of the stem is red, has good toughness and is not easy to break |
| Root hair | No new root hair growing | Clearly visible root hair growth |
| Root tip | Elongation of the root tip not clearly seen | Clearly visible root tip elongation |
The method treats 12 thousands of eucalyptus tissue culture bottle seedlings, the labor cost is 220 yuan, the matrix cost is 80 yuan, the water and electricity cost is 20 yuan, the other costs are 60 yuan, and the total cost is 380 yuan. The method treats 10 thousands of eucalyptus tissue culture bottle seedlings, each of which is 0.0032 yuan; in addition, the processing method has short time, and the packaging and transportation can be carried out after 7 days of processing.
The eucalyptus tissue culture bottle seedlings treated by the method are subjected to long-distance transport resistance effect analysis, and the eucalyptus tissue culture bottle seedlings which are not treated by the method are used as a control. Researches show that the eucalyptus tissue culture bottle seedlings are transported to a nursery garden in inkstone county of Yunnan province on a coach through Guangxi Liuzhou city, the transportation distance is about 900km, and the transportation time is 48 hours. After the fertilizer is transported to a nursery, the root rot rate is obviously lower than that of a control after the fertilizer is placed for 3 days, 5 days and 10 days; after the eucalyptus tissue culture seedlings are transported to a nursery garden and placed for 2 days, transplanting is carried out, and the survival rate after 30 days of transplanting is obviously higher than that of a control. Therefore, the eucalyptus tissue culture bottle seedlings subjected to the treatment method have obvious long-distance transportation resistance effect. The specific effect is analyzed by comparison, and is shown in the following table 6:
table 6: comparative analysis on long-distance transport resistance effect of eucalyptus tissue culture bottle seedlings treated by using method
The above-described embodiments are merely preferred embodiments of the present invention, and should not be construed as limiting the present invention, and the scope of the present invention is defined by the claims, and equivalents including technical features described in the claims. I.e., equivalent alterations and modifications within the scope hereof, are also intended to be within the scope of the invention.
Claims (2)
1. The long-distance transportation-resistant treatment method for the eucalyptus tissue culture bottle seedlings is characterized by comprising the following steps of:
1) taking out the eucalyptus tissue culture bottle seedlings from the culture bottle, taking each bottle of the eucalyptus tissue culture bottle seedlings as a bunch, cleaning the culture medium in clear water, and soaking the eucalyptus tissue culture bottle seedlings, which are cleaned with the culture medium, in yellow mud for 5-10 seconds; the yellow mud is prepared by fully and uniformly mixing 5-10 parts by weight of yellow core soil, 0.05-0.1 part by weight of potassium dihydrogen phosphate and 10-20 parts by weight of clear water; then sequentially adding 0.1-0.5 part of hydroxyethyl cellulose, 0.05-0.1 part of potassium polyacrylate and 0.002-0.005 part of carbendazim powder with the effective component content of 50 percent, and fully and uniformly mixing to prepare the bactericide;
2) vertically placing the bottle seedlings obtained in the step 1) on a seedling raising tray filled with a sterilized seedling raising matrix, covering the roots of the eucalyptus tissue culture bottle seedlings with yellow core soil, and covering a sunshade net; the seedling raising substrate is prepared by mixing 60-70 parts of red heart soil, 10-30 parts of coconut chaff, 3-4 parts of hydroxyethyl cellulose, 0.5-1 part of monopotassium phosphate and 0.5-1 part of polyaspartic acid potassium by weight;
3) after culturing for 2-3 days under a sunshade net, spraying a rooting deep sea fish oil diluent on leaves of the eucalyptus tissue culture bottle seedlings; and (3) continuing culturing for 3-5 days, wherein during culturing, the leaves and stems of the eucalyptus tissue culture bottle seedlings turn red slightly from green, and new roots grow out, namely, packaging and transporting, during packaging and transporting, the treated eucalyptus tissue culture bottle seedlings are placed in a foam box in a tidy and vertical manner, and then 70% of rhizoverine wettable powder solution diluted by 1000 times is sprayed on the leaf surfaces.
2. The method of claim 1, wherein: when the eucalyptus tissue culture bottle seedlings are placed in the step 2), each bottle of seedlings is cleaned with a culture medium and then is not disassembled into a single plant, and each bottle of seedlings is used as a bunch and placed on a seedling culture tray filled with a sterilized substrate; the placing line spacing is 5-10 cm.
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| CN105638419A (en) * | 2016-03-16 | 2016-06-08 | 蒋凡 | Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings |
| CN105993452A (en) * | 2016-05-18 | 2016-10-12 | 合肥公盈园艺科技有限公司 | Planting method capable of improving survival rate of seedlings |
| CN107056472A (en) * | 2017-05-10 | 2017-08-18 | 蚌埠市众星蔬果科技专业合作社联合社 | A kind of seed propagation method of fragrans seed |
| CN107384425A (en) * | 2017-08-08 | 2017-11-24 | 叶金仁 | Water-loss reducer of soil |
| CN108219797A (en) * | 2017-12-12 | 2018-06-29 | 浙江海洋大学 | A kind of soil moisturizing agent |
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2018
- 2018-09-07 CN CN201811046094.0A patent/CN109042215B/en not_active Expired - Fee Related
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