CN109042215A - A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling - Google Patents
A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling Download PDFInfo
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- CN109042215A CN109042215A CN201811046094.0A CN201811046094A CN109042215A CN 109042215 A CN109042215 A CN 109042215A CN 201811046094 A CN201811046094 A CN 201811046094A CN 109042215 A CN109042215 A CN 109042215A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G23/00—Forestry
- A01G23/02—Transplanting, uprooting, felling or delimbing trees
- A01G23/04—Transplanting trees; Devices for grasping the root ball, e.g. stump forceps; Wrappings or packages for transporting trees
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of processing methods of anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, the following steps are included: 1) take out Eucalyptus Tissue Cultured bottle seedling from culture bottle, every bottle of bottle seedling cleans culture medium in clear water, the Eucalyptus Tissue Cultured bottle seedling of clean culture medium is impregnated 5-10 seconds in yellow mud as pugil；2) bottle seedling that step 1) obtains uniformly is placed on the raising tray equipped with disinfection seedling medium according to the specification that line-spacing is 5-10cm, covers the root of Eucalyptus Tissue Cultured bottle seedling with the yellow soil that partial size is 1-3 millimeters, and cover sunshade net；3) it after being cultivated 2-3 days under sunshade net, takes root deep sea fish oil dilute solution to Eucalyptus Tissue Cultured bottle seedling blade spraying；Packing and transporting is carried out after continuing culture 3-5 days.It can make Eucalyptus Tissue Cultured bottle seedling during long-distance transport by this method, root is not perishable, and bottle seedling is less subject to damage, high survival rate when transplanting.
Description
Technical field
The present invention relates to seedling transportation field, especially a kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling.
Technical background
Eucalyptus (Eucalyptus robusta Smith) is Myrtaceae (Myrtaceae) plant.Eucalyptus is south China
The maximum fast-growing, high-yield woods of area's cultivated area, nursery is mainly bred by way of tissue culture, and produces Eucalyptus Tissue Cultured bottle seedling
Tissue culture factory is concentrated mainly on the Suixi County and Nanning, Fusui County, Laibin City, Liuzhou City in Guangdong.And tissue-culture container seedling
Seedling management after transplanting and transplanting is mainly distributed on the suitable plantings eucalyptus township such as Guangxi, Guangdong, Fujian, Yunnan, Guizhou, Sichuan
It is carried out in the nursery in town, for afforestation after turning out qualified nursery stock.Therefore, it is necessary to Eucalyptus Tissue Cultured bottle seedling is transported to each nursery to move
It plants.
Eucalyptus Tissue Cultured bottle seedling is in long-distance transport, since bottle seedling is grown in culture bottle always in cultivating process, growth
Environment is superior, and plant children is tender.Once after the culture medium of over cleaning root, it is placed in crowded foam transport case, was transporting
It is jolted etc. in the case where unfavorable factors in journey by atmosphere draught-free, car, Eucalyptus Tissue Cultured bottle seedling root is easily rotted, and eucalyptus is caused
Survival rate is low after setting tissue-culture container seedling transplanting, causes damages to seedling culture agriculture, while also constraining the remote of Eucalyptus Tissue Cultured bottle seedling
Transport and short-term storage.
A kind of processing method of anti-long-distance transport of Eucalyptus Tissue Cultured seedling, this method are disclosed in the patent No. 201610149963.7
It to be handled 1-3 time by outdoor water planting, and supplement pure originally Aquaponic 1-3 days after auxin in square plate is outwelled, then with growing
Element processing, every 4d-7d replace auxin in square plate, can packing and transporting after water planting 15-40 days.But this method is cumbersome,
It takes a long time, production cost is higher.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of processing method of anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling,
Make Eucalyptus Tissue Cultured bottle seedling during anti-long-distance transport, root is not perishable, and bottle seedling is less subject to damage, survival rate when transplanting
Height, and process is simple, production cost is low.
The technical solution adopted by the present invention is that a kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, including with
Lower step:
1) Eucalyptus Tissue Cultured bottle seedling is taken out from culture bottle, every bottle of bottle seedling is cleaned culture medium in clear water, will be washed as pugil
The Eucalyptus Tissue Cultured bottle seedling of net culture medium is impregnated 5-10 seconds in yellow mud；
2) bottle seedling that step 1) obtains is placed according to the specification homogeneous vertical that line-spacing is 5-10cm equipped with disinfection nursery base
On the raising tray of matter, the root of Eucalyptus Tissue Cultured bottle seedling is covered with the yellow soil that partial size is 1-3 millimeters, and cover sunshade net；
3) it after being cultivated 2-3 days under sunshade net, takes root deep sea fish oil dilution to Eucalyptus Tissue Cultured bottle seedling blade spraying；Continue to train
Packing and transporting is carried out after supporting 3-5 days.
Further, yellow mud described in step 1) is using 5-10 by weight parts of yellow soil, 0.05-0.1 parts of phosphorus
Acid dihydride potassium, 10-20 a part clear water mix well；0.1-0.5 parts of hydroxyethyl celluloses, 0.05-0.1 parts of polypropylene are successively added again
Sour potassium, the powder of carbendazim that 0.002-0.005 parts of effective component contents are 50% are manufactured after mixing well.
Further, seedling medium described in step 2) is using 60-70 parts of red heart soil by weight, coco bran 10-
30 parts, 3-4 parts of hydroxyethyl cellulose, 0.5-1 parts of potassium dihydrogen phosphate and 0.5-1 parts of poly-aspartic-acid potassium mix.
It further, when putting Eucalyptus Tissue Cultured bottle seedling in step 2), is not dismantled into after every bottle of bottle seedling is cleaned culture medium
Single plant, every bottle of bottle seedling are placed in as pugil equipped on the raising tray for having sterilized matrix；Putting line-spacing is 5-10cm.
Further, when continuing culture in step 3), the blade and stem of Eucalyptus Tissue Cultured bottle seedling slightly turn red by green, and have
I.e. packing and transporting after new root is born.
Further, when packing and transporting, will treated that Eucalyptus Tissue Cultured bottle seedling is neatly vertically arranged is placed in bubble chamber, then
1000 times of 70% root-rot spirit wettable powder agent solution is diluted in foliage-spray.
The method have the advantages that:
1. in yellow mud and seedling medium formula, potassium dihydrogen phosphate, hydroxyethyl cellulose, polyacrylic acid potassium and poly- lucid asparagus
Propylhomoserin potassium main function is not allow the dehydration of Eucalyptus Tissue Cultured bottle seedling and withered as water-retaining agent, and bottle seedling is promoted to train on raising tray
Rapid Rooting when supporting；Meanwhile potassium dihydrogen phosphate can make the stalk of Eucalyptus Tissue Cultured bottle seedling and blade it is seasoned and in transit not vulnerable to
Damage.
2. the stickiness of yellow soil is strong, it is easy to bind with Eucalyptus Tissue Cultured bottle seedling root system after being made into yellow mud；The hole of red heart soil
Degree is big, good permeability, and Eucalyptus Tissue Cultured bottle seedling root system is easy to raw new root.
3. the principle of entire processing method is in the present invention: by making bottle seedling quick to Eucalyptus Tissue Cultured bottle seedling Short-term Culture
It takes root；And it is seasoned by the stalk and blade of Eucalyptus Tissue Cultured bottle seedling by applying potassium dihydrogen phosphate, so that bottle seedling be made to be not easy in transit
It is impaired.
Specific embodiment
The present invention is further illustrated below with reference to embodiment, but the scope of protection of present invention is not limited to implement
The range of example statement.
Embodiment 1:
A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, steps are as follows:
(1) Eucalyptus Tissue Cultured bottle seedling is taken out from culture bottle, every bottle of bottle seedling cleans culture medium as pugil in clear water, will
The Eucalyptus Tissue Cultured bottle seedling for cleaning culture medium is impregnated 10 seconds in yellow mud.Yellow mud is to weigh 10kg yellow soil fine crushing, 0.1kg phosphorus
Acid dihydride potassium, 20kg clear water mix well；Successively addition 0.5kg hydroxyethyl cellulose, 0.1kg polyacrylic acid potassium, 5g are effective again
The powder of carbendazim that component content is 50%, it is manufactured after mixing well.
(2) by the Eucalyptus Tissue Cultured bottle seedling after impregnating in yellow mud, dress is uniformly placed according to the specification that line-spacing is 10cm
Have on the raising tray of seedling medium, each raising tray is put 60 bottles of bottle seedlings (1500 plants), and the gold zone for being 1-3 millimeters with partial size
The root of soil covering Eucalyptus Tissue Cultured bottle seedling, and cover sunshade net；The seedling medium is mixed by the configuration of following mass parts: grain
The red heart soil 60kg, coco bran 30kg, hydroxyethyl cellulose 3kg that diameter is 1-3 millimeters, potassium dihydrogen phosphate 1kg, poly-aspartic-acid potassium
1kg。
(3) after being cultivated 3 days under sunshade net, the day one-for being 500 times to Eucalyptus Tissue Cultured bottle seedling blade spraying dilution volume multiple
It takes root deep sea fish oil solution.
(4) continue under sunshade net culture 5 days after, by Eucalyptus Tissue Cultured bottle seedling be neatly placed in 50cm (length) × 30cm (width) ×
In 10cm (height) bubble chamber, each bubble chamber places 5000 plants of Eucalyptus Tissue Cultured seedlings, and foliage-spray dilutes 1000 times of 70% root
Rotten spirit wettable powder agent solution.
Eucalyptus Tissue Cultured bottle seedling is by this method treated seedling growth character and anti-long-distance transport effect comparative analysis:
Eucalyptus Tissue Cultured bottle seedling is after this method is handled, the life of the Eucalyptus Tissue Cultureds bottle seedling such as blade, stem, root hair, tip of a root
Long character obviously tends to ageing.Before and after the processing, the growth traits of Eucalyptus Tissue Cultured bottle seedling describes table 1 specific as follows to this method:
Table 1: the growth traits comparative analysis of Eucalyptus Tissue Cultured bottle seedling before and after the processing
| Before this method processing | After this method processing | |
| Blade | Blade is in light green color, and blade seems that children is tender | Blade is in bronzing, the obvious ageing of blade |
| Stem | Entire stem is in light green color, frangibility | Stem head takes on a red color, and good toughness is not easily broken |
| Root hair | It there are no new root staple length to go out | Root staple length can be clearly seen to go out |
| The tip of a root | Fail clearly to see tip of a root elongation | Tip of a root elongation can clearly be seen |
This method handles 100,000 plants of Eucalyptus Tissue Cultured bottle seedling, and cost of labor is 200 yuan, and matrix cost is 80 yuan, water power cost
It is 20 yuan, other costs are 50 yuan, add up to 350 yuan.This method handles 100,000 plants of Eucalyptus Tissue Cultured bottle seedling, 0.0035 yuan every plant；This
Outside, the treatment method time is short, and processing 8 days can packing and transporting.
Anti- long-distance transport effect analysis is carried out by the Eucalyptus Tissue Cultured bottle seedling that this method is handled, is handled without this method
As control.The study found that the Eucalyptus Tissue Cultured bottle seedling is transported through Liuzhou city, Guangxi province to Heyuan City of Guangdong Province purple on coach
The town golden county Huang Tang nursery, transportation range about 800km, haulage time are 40 hours.Transport reach nursery after, place 3 days, 5 days,
Root-rot rate after 10 days, which is substantially less than, to be compareed；It after Eucalyptus Tissue Cultured seedling transports to nursery, is transplanted after placing 2 days, after transplanting 30 days
Survival rate is significantly higher than control.As it can be seen that the Eucalyptus Tissue Cultured bottle seedling by treatment method carries out anti-long-distance transport significant effect.Tool
Body effect comparative analysis, see the table below 2:
Table 2: Eucalyptus Tissue Cultured bottle seedling is by this method treated anti-long-distance transport effect comparative analysis
Embodiment 2:
A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, steps are as follows:
(1) Eucalyptus Tissue Cultured bottle seedling is taken out from culture bottle, every bottle of bottle seedling cleans culture medium as pugil in clear water, will
The Eucalyptus Tissue Cultured bottle seedling for cleaning culture medium is impregnated 5 seconds in yellow mud.Yellow mud is to weigh 5kg yellow soil fine crushing, 0.05kg phosphorus
Acid dihydride potassium, 10kg clear water mix well；Successively addition 0.1kg hydroxyethyl cellulose, 0.05kg polyacrylic acid potassium, 2g are effective again
The powder of carbendazim that component content is 50%, it is manufactured after mixing well.
(2) it by the Eucalyptus Tissue Cultured bottle seedling after being impregnated in yellow mud, is uniformly placed in and is equipped with according to the specification that line-spacing is 5cm
On the raising tray of seedling medium, each raising tray puts 60 bottle seedlings (1500 plants), and is covered with the yellow soil that partial size is 1-3 millimeters
The root of lid Eucalyptus Tissue Cultured bottle seedling, and cover sunshade net；The seedling medium is mixed by the configuration of following mass parts: partial size is
1-3 millimeters of red heart soil 70kg, coco bran 20kg, hydroxyethyl cellulose 4kg, potassium dihydrogen phosphate 0.5kg, poly-aspartic-acid potassium
0.5kg。
(3) after cultivating 2 days under sunshade net, the day one-for being 500 times to Eucalyptus Tissue Cultured bottle seedling blade spraying volume multiple is taken root
Deep sea fish oil solution.
(4) continue under sunshade net culture 3 days after, by Eucalyptus Tissue Cultured bottle seedling be neatly placed in 50cm (length) × 30cm (width) ×
In 10cm (height) bubble chamber, each bubble chamber places 5000 plants of Eucalyptus Tissue Cultured seedlings, and foliage-spray dilutes 1000 times of 70% root
Rotten spirit wettable powder agent solution.
Eucalyptus Tissue Cultured bottle seedling is by this method treated seedling growth character and anti-long-distance transport effect comparative analysis:
Eucalyptus Tissue Cultured bottle seedling is after this method is handled, the life of the Eucalyptus Tissue Cultureds bottle seedling such as blade, stem, root hair, tip of a root
Long character obviously tends to ageing.Before and after the processing, the growth traits of Eucalyptus Tissue Cultured bottle seedling describes table 3 specific as follows to this method:
Table 3: the growth traits comparative analysis of Eucalyptus Tissue Cultured bottle seedling before and after the processing
This method handles 80,000 plants of Eucalyptus Tissue Cultured bottle seedling, and cost of labor is 180 yuan, and matrix cost is 80 yuan, and water power cost is
20 yuan, other costs are 40 yuan, add up to 320 yuan.This method handles 100,000 plants of Eucalyptus Tissue Cultured bottle seedling, 0.004 yuan every plant；In addition,
The processing method time is short, and processing 5 days can packing and transporting.
Anti- long-distance transport effect analysis is carried out by the Eucalyptus Tissue Cultured bottle seedling that this method is handled, is handled without this method
As control.The study found that the Eucalyptus Tissue Cultured bottle seedling is transported through Liuzhou city, Guangxi province to Sichuan Province's Yibin City on coach
Nursery, transportation range about 1000km, haulage time are 48 hours.Root after transport reaches nursery, after placing 3 days, 5 days, 10 days
Rotten rate is substantially less than and compares；It after Eucalyptus Tissue Cultured seedling transports to nursery, is transplanted after placing 2 days, the survival rate after transplanting 30 days is significant
Higher than control.As it can be seen that the Eucalyptus Tissue Cultured bottle seedling by treatment method carries out anti-long-distance transport significant effect.Specific effect compares
Analysis, see the table below 4:
Table 4: Eucalyptus Tissue Cultured bottle seedling is by this method treated anti-long-distance transport effect comparative analysis
Embodiment 3:
A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, steps are as follows:
(1) Eucalyptus Tissue Cultured bottle seedling is taken out from culture bottle, every bottle of bottle seedling cleans culture medium as pugil in clear water, will
The Eucalyptus Tissue Cultured bottle seedling for cleaning culture medium is impregnated 8 seconds in yellow mud.Yellow mud is to weigh 7.5kg yellow soil fine crushing, 0.08kg
Potassium dihydrogen phosphate, 15kg clear water mix well；0.3kg hydroxyethyl cellulose, 0.08kg polyacrylic acid potassium are successively added again, and 4g has
The powder of carbendazim that component content is 50% is imitated, it is manufactured after mixing well.
(2) it by the Eucalyptus Tissue Cultured bottle seedling after being impregnated in yellow mud, is uniformly placed in and is equipped with according to the specification that line-spacing is 5cm
On the raising tray of seedling medium, each raising tray puts 60 bottle seedlings (1500 plants), and is covered with the yellow soil that partial size is 1-3 millimeters
The root of lid Eucalyptus Tissue Cultured bottle seedling, and cover sunshade net；The seedling medium is mixed by the configuration of following mass parts: partial size is
1-3 millimeters of red heart soil 65kg, coco bran 24kg, hydroxyethyl cellulose 3.5kg, potassium dihydrogen phosphate 1kg, poly-aspartic-acid potassium
1kg。
(3) after cultivating 3 days under sunshade net, the day one-for being 500 times to Eucalyptus Tissue Cultured bottle seedling blade spraying volume multiple is taken root
Deep sea fish oil solution.
(4) continue under sunshade net culture 4 days after, by Eucalyptus Tissue Cultured bottle seedling be neatly placed in 50cm (length) × 30cm (width) ×
In 10cm (height) bubble chamber, each bubble chamber places 5000 plants of Eucalyptus Tissue Cultured seedlings, 1000 times of foliage-spray of 70% root-rot spirit
Wettable powder agent solution.
Eucalyptus Tissue Cultured bottle seedling is by this method treated seedling growth character and anti-long-distance transport effect comparative analysis:
Eucalyptus Tissue Cultured bottle seedling is after this method is handled, the life of the Eucalyptus Tissue Cultureds bottle seedling such as blade, stem, root hair, tip of a root
Long character obviously tends to ageing.Before and after the processing, the growth traits of Eucalyptus Tissue Cultured bottle seedling describes table 5 specific as follows to this method:
Table 5: the growth traits comparative analysis of Eucalyptus Tissue Cultured bottle seedling before and after the processing
| Before this method processing | After this method processing | |
| Blade | Blade is in light green color, and blade seems that children is tender | Blade is in bronzing, the obvious ageing of blade |
| Stem | Entire stem is in light green color, frangibility | Stem head takes on a red color, and good toughness is not easily broken |
| Root hair | It there are no new root staple length to go out | Root staple length can be clearly seen to go out |
| The tip of a root | Fail clearly to see tip of a root elongation | Tip of a root elongation can clearly be seen |
This method handles 120,000 plants of Eucalyptus Tissue Cultured bottle seedling, and cost of labor is 220 yuan, and matrix cost is 80 yuan, water power cost
It is 20 yuan, other costs are 60 yuan, add up to 380 yuan.This method handles 100,000 plants of Eucalyptus Tissue Cultured bottle seedling, 0.0032 yuan every plant；This
Outside, the processing method time is short, and processing 7 days can packing and transporting.
Anti- long-distance transport effect analysis is carried out by the Eucalyptus Tissue Cultured bottle seedling that this method is handled, is handled without this method
As control.The study found that the Eucalyptus Tissue Cultured bottle seedling is transported through Liuzhou city, Guangxi province to Yunnan Province Yanshan County on coach
Nursery, transportation range about 900km, haulage time are 48 hours.Root-rot after transport reaches nursery, after placing 3 days, 5 days, 10 days
Rate is substantially less than and compares；It after Eucalyptus Tissue Cultured seedling transports to nursery, is transplanted after placing 2 days, the survival rate after transplanting 30 days is significantly high
In control.As it can be seen that the Eucalyptus Tissue Cultured bottle seedling by treatment method carries out anti-long-distance transport significant effect.Specific effect compares point
Analysis, see the table below 6:
Table 6: Eucalyptus Tissue Cultured bottle seedling is by this method treated anti-long-distance transport effect comparative analysis
The above embodiments are only the preferred technical solution of the present invention, and are not construed as limitation of the invention, this hair
Bright protection scope should be with the technical solution of claim record, technical characteristic in the technical solution recorded including claim
Equivalents are protection scope.Equivalent replacement i.e. within this range is improved, also within protection scope of the present invention.
Claims (6)
1. a kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling, which comprises the following steps:
1) Eucalyptus Tissue Cultured bottle seedling is taken out from culture bottle, every bottle of bottle seedling cleans culture medium, by clean training as pugil in clear water
The Eucalyptus Tissue Cultured bottle seedling for supporting base is impregnated 5-10 seconds in yellow mud；
2) the bottle seedling vertical display that step 1) obtains covers eucalyptus with yellow soil on the raising tray equipped with disinfection seedling medium
The root of tissue-culture container seedling, and cover sunshade net；
3) it after being cultivated 2-3 days under sunshade net, takes root deep sea fish oil dilution to Eucalyptus Tissue Cultured bottle seedling blade spraying；Continue to cultivate 3-
Packing and transporting is carried out after 5 days.
2. according to the method described in claim 1, it is characterized by: yellow mud described in step 1) is using 5- by weight
10 parts of yellow soils, 0.05-0.1 parts of potassium dihydrogen phosphates, 10-20 parts of clear water mix well；0.1-0.5 parts of ethoxys are successively added again
Cellulose, 0.05-0.1 parts of polyacrylic acid potassiums, the powder of carbendazim that 0.002-0.005 parts of effective component contents are 50% are sufficiently mixed
It is manufactured after even.
3. according to the method described in claim 1, it is characterized by: seedling medium described in step 2 is that use is red by weight
60-70 parts of cubsoil, 10-30 parts of coco bran, 3-4 parts of hydroxyethyl cellulose, 0.5-1 parts of potassium dihydrogen phosphate and poly-aspartic-acid potassium
0.5-1 parts mix.
4. according to the method described in claim 1, it is characterized by: being by every bottle when putting Eucalyptus Tissue Cultured bottle seedling in step 2
Bottle seedling does not dismantle into single plant after cleaning culture medium, and every bottle of bottle seedling is placed in as pugil equipped with the raising tray for having sterilized matrix
On；Putting line-spacing is 5-10cm.
5. according to the method described in claim 1, it is characterized by: continue in step 3) culture when, the leaf of Eucalyptus Tissue Cultured bottle seedling
Piece and stem slightly turn red by green, and i.e. packing and transporting after having new root to bear.
6. method described in -5 any one according to claim 1, it is characterised in that:, will treated eucalyptus when packing and transporting
Tissue-culture container seedling is neatly vertically arranged and is placed in bubble chamber, and 1000 times of 70% root-rot spirit wettable powder is then diluted in foliage-spray
Agent solution.
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