CN102812893A - Culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles - Google Patents
Culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles Download PDFInfo
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- CN102812893A CN102812893A CN2012102902665A CN201210290266A CN102812893A CN 102812893 A CN102812893 A CN 102812893A CN 2012102902665 A CN2012102902665 A CN 2012102902665A CN 201210290266 A CN201210290266 A CN 201210290266A CN 102812893 A CN102812893 A CN 102812893A
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Abstract
The invention provides a culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles in water. The culture method includes the following steps: the needle fascicles of a slash pine x Caribbean pine F1 hybrid seed tree are taken and disinfected with carbendazim solution for 2 to 3 hours; the bases of the needle fascicles are cut; the bases of the needle fascicles are immersed in NAA solution for 12 to 16 hours, and the needle fascicles are washed with clean water for 2 to 3 times and vertically placed in glass bottles loaded with nutrient solution; the needle fascicles are cultured in a plastic greenhouse which is shaded by a shading net, and the nutrient solution is replaced once each week. By researching the affection of the age of the seed tree of the slash pine x Caribbean pine F1 hybrid needle fascicles, the concentration of growth regulator, the ingredients of the nutrient solution and other factors on the rooting of the needle fascicles, the invention obtains the optimal culture method for rooting the needle fascicles in water, and the rooting rate of the needle fascicles reaches 91.96 percent. The material source is rich, the operating method is simple, and is convenient to use, and the rooting rate is high. The operating process cannot pollute the environment, and the culture method is safe to use, and can be used for rooting needle fascicles of pine trees in water.
Description
Affiliated technical field
The present invention relates to the water culture technology of taking root, more particularly the present invention relates to a kind of wet cultural method that adds pine needle Shu Shenggen, it is applicable to that the needle-leaved bundle water planting of pine tree takes root.
Background technology
Wet-land pine tree (Pinus elliottii Engelm) * pinus caribaea (P.caribaea Morelet) F
1Hybrid (being called for short the wet pine that adds) has characteristics such as speed is given birth to, amount of growth is big, trunk is perfectly straight, branch is good, cold tolerance is strong, is adapted at China's south afforestation and promotes.
Because wet-land pine tree and pinus caribaea's hybridization affinity is low; Cause that hybrid seed yields poorly, nursery stock quantity is few; Nursery stock output and social demand are particularly thorny; For satisfying the production needs, the forest-science academy, Guangdong Province carried out tender skill cottage propagation research from 1996, the basic hybrid pine scale softwood cutting propagation technology of grasping by 2000.Wet add the pine cuttage seedling with the seedling forestation comparative test result shows, both afforestation effect difference is little, therefore must walk sexual creation, the approach of asexual utilization in wet adding in the loose good hybrid popularization.And the needle-leaved bundle base portion of pine tree includes growing point, possesses the potentiality of germination and the ability of taking root, and needle-leaved bundle is grown seedlings and had the numerous coefficient of bigger expansion than epicormic branch cutting, expands at choiceness in the preservation of numerous popularization, excellent genes resource to have higher advantage.
Summary of the invention
The object of the invention: through the wet water planting that adds the pine needle bundle, increase the quantity of choiceness nursery stock, quicken the process of afforestation improved variety.Wetting adds the cultural method that pine needle Shu Shuipei is taken root, and it is characterized in that:
The elite stand age of tree of gathering needle-leaved bundle is 2～5 years
The needle-leaved bundle disinfectant is 0.5% carbendazim solution
Growth regulator NAA concentration is 50～125mg/kg
The weight percentage of each component of nutrient solution is:
In the foregoing invention scheme, being characterized as of the wet preferred cultural method that adds the pine needle bundle:
The elite stand origin of gathering needle-leaved bundle is cuttage seeding or seedling, and the age of tree is 2～3 years
The carbendazim solution of needle-leaved bundle employing 0.5% soaks the above 0.6cm of its base portion 2.5h of place
Growth regulator NAA concentration is 50～100mg/kg
The weight percentage of each component of nutrient solution is:
The present invention is wet to add being characterized as of best cultural method of pine needle bundle:
The elite stand origin of gathering needle-leaved bundle is cuttage seeding, and the age of tree is 3 years
The carbendazim solution of needle-leaved bundle employing 0.5% soaks its base portion 0.6cm 2h of place
Growth regulator NAA concentration is 100mg/kg, soaks the above 0.6cm of the needle-leaved bundle base portion 16h of place
The weight percentage of each component of nutrient solution is:
The present invention is through studying the influence that wet elite stand source that adds the pine needle bundle and age, growth regulating agent concentration, nutrient solution prescription etc. are taken root to needle-leaved bundle, obtains wetly to add the best cultural method that pine needle Shu Shuipei is taken root, and the needle-leaved bundle rooting rate reaches 91.96%.Material source of the present invention is abundant, and method of operating is simple, and is easy to use, and rooting rate is high.In addition, the cultural method that needle-leaved bundle water planting of the present invention is taken root can not pollute environment in operating process, and is safe in utilization, and the needle-leaved bundle water planting that can be used for pine tree is taken root.
That gathers needle-leaved bundle wetly adds loose elite stand source and the age of tree is big to the rooting rate influence, and rooting rate has to increase with the elite stand age of tree and descends, and rooting rate is higher in the period of 2～5, wherein, the highest with the originate from needle-leaved bundle rooting rate of elite stand of livings cuttage in 3 years;
Growth regulator NAA concentration effect in 50～125mg/kg scope is remarkable, and wherein, 100mg/kg is best;
Best nutrient solution prescription comprises following component: ammonium nitrate 0.004%; Boric acid 0.0075%; Cobastab
10.002%; Water: 99.9865%.
Optimized technical scheme of the present invention is:
A) a kind of wet cultural method that adds pine needle Shu Shenggen is characterized in that:
The elite stand origin of gathering needle-leaved bundle is cuttage seeding, and the age of tree is 3 years,
Needle-leaved bundle adopts 0.5% carbendazim solution sterilization 2h
Growth regulator NAA concentration is that 100mg/kg handles 16h
The weight percentage of each component of nutrient solution is:
The cultural method that should wet add pine needle Shu Shenggen is following; Get the needle-leaved bundle of 3 years living cuttage seeding source elite stands; Be put in and 0.5% carbendazim solution is housed soaks the above 0.6cm of its base bottom 2h of place; After using concentration to soak the above 0.6cm of the needle-leaved bundle base bottom 16h of place again, towards Xian 3 times, be put in and foster liquid be housed (constituent is: ammonium nitrate 0.004% with clear water as the NAA liquid of 100mg/kg; Boric acid 0.0075%; Cobastab
10.002%; Water: in the vial 99.9865%); Place above-mentioned vial on the culturing rack in the plastic tunnel that shades with shading net and cultivate, change nutrient solution weekly 1 time.
B) a kind of wet cultural method that adds pine needle Shu Shenggen is characterized in that:
The elite stand origin of gathering needle-leaved bundle is cuttage seeding, and the age of tree is 3 years,
Needle-leaved bundle adopts 0.5% carbendazim solution sterilization 2.5h
Growth regulator NAA concentration is that 50mg/kg handles 16h
The weight percentage of each component of nutrient solution is:
The cultural method that should wet add pine needle Shu Shenggen is following; Get the needle-leaved bundle of 3 years living cuttage seeding source elite stands; Be put in and 0.5% carbendazim solution is housed soaks its base portion 2.5h; After using concentration to soak needle-leaved bundle base portion 16h again, towards Xian 3 times, be put in and foster liquid be housed (constituent is: ammonium nitrate 0.004% with clear water as 50mg/kgNAA liquid; Boric acid 0.0075%; Cobastab
10.002%; Water: in the vial 99.9865%); Place above-mentioned vial on the culturing rack in the plastic tunnel that shades with shading net and cultivate, change nutrient solution weekly 1 time.
C) a kind of wet cultural method that adds pine needle Shu Shenggen is characterized in that:
The elite stand origin of gathering needle-leaved bundle is seedling, and the age of tree is 3 years,
Needle-leaved bundle adopts 0.5% carbendazim solution sterilization 3h
Growth regulator NAA concentration is that 100mg/kg handles 12～16h
The weight percentage of each component of nutrient solution is:
The cultural method that should wet add pine needle Shu Shenggen is following; Get the needle-leaved bundle of 3 years living seedling source elite stands; Be put in and 0.5% carbendazim solution is housed soaks its base portion 3h; After using concentration to soak needle-leaved bundle base portion 12～16h again, towards Xian 3 times, be put in and foster liquid be housed (constituent is: ammonium nitrate 0.004% with clear water as 100mg/kgNAA liquid; Boric acid 0.0075%; Cobastab
10.002%; Water: in the vial 99.9865%); Place above-mentioned vial on the culturing rack in the plastic tunnel that shades with shading net and cultivate, change nutrient solution weekly 1 time.
Embodiment
Elite stand source, NAA concentration and the nutrient solution prescription of gathering needle-leaved bundle is at the wet effect as shown in the table embodiment of pine needle Shu Shuipei in taking root that add:
Table 1 separate sources (life in 3 years) elite stand and NAA concentration and nutrient composition are made the result mutually
| Embodiment | The elite stand source | NAA concentration (mg/kg) | Nutrient solution key component (unit: mg/kg) | Rooting rate (%) |
| 1 | Cuttage seeding | 100 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 91.96 |
| 2 | Cuttage seeding | 50 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 86.87 |
| 3 | Seedling | 100 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 86.87 |
| 4 | Seedling | 50 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 79.96 |
| 5 | Cuttage seeding | 100 | 40(KH 2PO 4)+75(H 3BO 4)+20(VB 1) | 54.79 |
| 6 | Seedling | 100 | 40(KH 2PO 4)+75(H 3BO 4)+20(VB 1) | 53.79 |
| 7 | Cuttage seeding | 125 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 43.81 |
| 8 | Seedling | 125 | 40(NH 4NO 3)+75(H 3BO 4)+20(VB 1) | 42.49 |
| 9 | Seedling | 50 | 40(KH 2PO 4)+75(H 3BO 4)+20(VB 1) | 38.24 |
| 10 | Seedling | 125 | 40(KH 2PO 4)+75(H 3BO 4)+20(VB 1) | 19.44 |
The practical implementation method is:
With embodiment 1 is example, with thumb, forefinger beat easily life in 3 years in cuttage source wet add loose elite stand last 1 year summer shoot the needle-leaved bundle of middle and lower part, be bundled into bundle with rope, be put in be equipped with high 0.5% carbendazim solution of 0.6cm barrel in soak 2～3h; Fall the base portion (promptly needle-leaved bundle is done and cut the base processing) of belt leather then at the intersection straight snips of primary leaf and base portion with scissors; Be put in soak 16h in the vial that the high 100mg/kgNAA solution of 0.6cm is housed after, take out with flushing with clean water 3 times, place that (constituent is: ammonium nitrate 0.004% in that nutrient solution is housed vertically; Boric acid 0.0075%; Cobastab
10.002%; Water: in the vial 99.9865%); Place above-mentioned vial on the culturing rack in the plastic tunnel that shades with shading net and cultivate, change nutrient solution weekly 1 time.Through sending out roots in 4～5 weeks in base bottom.
Claims (3)
1. a wet cultural method that adds pine needle Shu Shenggen the steps include: that the wet needle-leaved bundle that adds loose elite stand is as propagating materials;
Soak needle-leaved bundle base portion 2～3h with disinfectant; Soak needle-leaved bundle base portion 12～16h with NAA solution then, use flushing with clean water again 2～3 times;
Cultivate with nutrient solution at last, change nutrient solution weekly 1 time; Through above-mentioned processing, obtain the needle-leaved bundle of taking root; It is characterized in that:
The elite stand age of tree of gathering needle-leaved bundle is 2～5 years
The needle-leaved bundle disinfectant is 0.5% carbendazim solution
Growth regulator NAA concentration is 50～125mg/kg
The weight percentage of each component of nutrient solution is:
2. the wet cultural method that adds the pine needle bundle according to claim 1 is characterized in that: the elite stand origin of gathering needle-leaved bundle is cuttage seeding or seedling, and the age of tree is 2～3 years
The carbendazim solution of needle-leaved bundle employing 0.5% soaks the above 0.6cm of the base portion 2.5h of place
Growth regulator NAA concentration is 50～100mg/kg
The weight percentage of each component of nutrient solution is:
3. the wet cultural method that adds the pine needle bundle according to claim 1 is characterized in that:
The elite stand origin of gathering needle-leaved bundle is cuttage seeding, and the age of tree is 3 years
The carbendazim solution of needle-leaved bundle employing 0.5% soaks the above 0.6cm of its base portion 2h of place
Growth regulator NAA concentration is 100mg/kg, soaks needle-leaved bundle base portion 16h
The weight percentage of each component of nutrient solution is:
.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145305A (en) * | 2015-09-16 | 2015-12-16 | 湖北省林业科学研究院 | Water culture seedling method for slash pine big tree needle fascicles |
| CN105766583A (en) * | 2016-03-28 | 2016-07-20 | 烟台市林业科学研究所 | Water culture rooting method of needle-leaved bundles of Pinus thunbergii and P.densiflora hybrid |
| CN116219052A (en) * | 2022-09-22 | 2023-06-06 | 北京林业大学 | Marker gene Pt7G61650 for identifying different periods of nutrient growth of slash pine and application method thereof |
Citations (1)
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| CN1491535A (en) * | 2002-10-25 | 2004-04-28 | 中国科学院沈阳应用生态研究所 | Root inductive method for microbody reproduction of Japan dahurian larch |
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2012
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1491535A (en) * | 2002-10-25 | 2004-04-28 | 中国科学院沈阳应用生态研究所 | Root inductive method for microbody reproduction of Japan dahurian larch |
Non-Patent Citations (5)
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| 乐海波等: "油松针叶束繁殖研究", 《湖北林业科技》 * |
| 吴敏等: "柴松针叶束水培生根的研究", 《西北林学院学报》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145305A (en) * | 2015-09-16 | 2015-12-16 | 湖北省林业科学研究院 | Water culture seedling method for slash pine big tree needle fascicles |
| CN105145305B (en) * | 2015-09-16 | 2017-09-26 | 湖北省林业科学研究院 | A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree |
| CN105766583A (en) * | 2016-03-28 | 2016-07-20 | 烟台市林业科学研究所 | Water culture rooting method of needle-leaved bundles of Pinus thunbergii and P.densiflora hybrid |
| CN105766583B (en) * | 2016-03-28 | 2018-09-28 | 烟台市林业科学研究所 | A kind of black Japanese red pine needle-leaved bundle water planting rooting method |
| CN116219052A (en) * | 2022-09-22 | 2023-06-06 | 北京林业大学 | Marker gene Pt7G61650 for identifying different periods of nutrient growth of slash pine and application method thereof |
| CN116219052B (en) * | 2022-09-22 | 2023-09-05 | 北京林业大学 | Marker gene Pt7G61650 for identifying different periods of nutrient growth of slash pine and application method thereof |
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Application publication date: 20121212 |