CN105766583A - Water culture rooting method of needle-leaved bundles of Pinus thunbergii and P.densiflora hybrid - Google Patents
Water culture rooting method of needle-leaved bundles of Pinus thunbergii and P.densiflora hybrid Download PDFInfo
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Abstract
The invention discloses a water culture rooting method of needle-leaved bundles of Pinus thunbergii and P.densiflora hybrid. The method is characterized by comprising steps as follows: preparation of water culture facilities and water culture carriers, collection of the needle-leaved bundles, storage of the needle-leaved bundles in sand, preparation of a nutrient solution, hormone treatment, penetration and fixation of the needle-leaved bundles, temperature and humidity control and the like. According to the method, the utilization rate and the efficacy of the needle-leaved bundles are remarkably increased, the water culture carriers are changed, and the suspension problem of the needle-leaved bundles is solved; the yellowing problem caused by malnutrition of the needle-leaved bundles is solved due to improvement of the matching ratio of a modifier to the nutrient solution; requirements for the water culture field are not strict, the temperature and the humidity are better controlled, and the rooting time is remarkably shortened.
Description
Technical field:
The present invention relates to nursery subject in forestry technology field (code 2202220), specifically black Pinus densiflora needle-leaved bundle water planting rooting method；For the Pinus seeds nursery stock asexual propagation such as Pollen pini thunbergii, Pinus densiflora.
Background technology:
Pinus densiflora is important afforestation vanguard tree seed, is one of the Main Constructive Species of coastal area, warm temperate zone warm nature Coniferous forest.What Pollen pini thunbergii was existing century-old introduces a fine variety history, obtains large-area applications due to its anti-pine moth, Matsumura pine scale harm, for the dominant tree of Northern Coastal Region shelterbelt construction.
Black Pinus densiflora is to utilize Pollen pini thunbergii to make improved seeds maternal, that Pinus densiflora does male parent selection-breeding, has that growth is fast, strong stress resistance, a feature that genetic gain is big.The raw height increament of its 7a is beyond compareing black Pinus densiflora F1Population mixture 52.75%～123.32%, girth growth amount is than compareing big 1.65～2.70cm, and even crown diameter is 1.22～1.44 times of comparison.It is suitable for the regional popularization and application in Pollen pini thunbergii growth.
Utilize degeneration-resistant, fast-growing, the adaptable black Pinus densiflora select tree material of selection-breeding; carry out asexual propagation to be protected by utilizing; improve breeding coefficient, be realize elite germplasm kindization and construction clonal seed orchard effective way, be basis and the key of eco forest systerm construction.
Needle-leaved bundle water planting nursery, is vegetative a kind of mode, and needle-leaved bundle water planting research is achieved with remarkable progress, and Pinus massoniana Lamb, pinus elliottii, Pinus tabuliformis, pinus caribaea and Chai Song achieve successfully, but Pollen pini thunbergii water planting is unsuccessful.
Li Fuxiu etc., 3a raw Pinus kesiya Royle ex Gordon var. langbianensis (A.Chev) Gaussen needle-leaved bundle rooting rate is the highest, and best nutritional liquid is 75mg/kgH3BO3+ 20mg/kgNH4NO3+ 20mg/kgVB1, indoor sand is hidden, and temperature controls at 5～8 DEG C, time 30d；Qie Ji, water planting in hot house, control temperature 15～25 DEG C, humidity more than 85%.7d changes one time of nutrition liquid.Rootage duration is 30d the earliest, and 40～50d reaches peak of taking root.
Yang Chenggui etc., 2a are raw, and Needle of Slash Pine bundle rooting rate is the highest, husky Tibetan 20～30d；Loading culture plate water planting in hot house, controlling temperature system is 16～28 DEG C, humidity more than 80%.
Che Shaohui, Pinus tabuliformis 2a Rhizoma Anemarrhenae's tree needle-leaved bundle rooting rate is the highest, and sampling time is best with December, and rooting rate is up to 50%.The sand of needle-leaved bundle hides temperature about 5 DEG C, and the husky Tibetan time is with 30～35d for the best;Cut base and can improve rooting rate.Suitable nutrient solution prescription is 50mg L-1Boric acid+28mg L-1Ammonium nitrate+30mg L-1Vitamin B1+20mg·L-1Potassium dihydrogen phosphate, maintains the experimental enviroment of 16-24 DEG C and may advantageously facilitate the generation of adventitious root.20d produces adventitious root, and speed of taking root after 30d is accelerated.
Wu Huishan etc., wet to add pinaster age be 2～3a, needle-leaved bundle is cut base and processes, adopt the carbendazim solution leaching base portion above 0.6cm place 2.5h of 0.5%, and growth regulator NAA concentration is 50～100mg/kg, and nutritional solution is 75mg/kgH3BO3+ 40mg/kgNH4NO3+ 20mg/kgVB1, needle-leaved bundle is put in equipped with in the vial of nutrient solution, and vial is placed on the culturing rack in the hot house shaded with shading net and cultivates, and every 7d changes 1 nutritional solution.Will send out roots in base bottom through 28～35d.
Being permitted Asia etc., the pinus elliottii age of tree is 8～10a, and adopting mass concentration is the KMnO of 0.2～0.5%4Solution disinfection, indoor sand is hidden and lid thin film moisturizing, and temperature controls at 5～8 DEG C, time 25～35d；It is modulated into water planting frame with group training frame, in mid-February, Qie Ji, puts in the root element solution prepared in advance, soak time is 12～16h, water planting bottle made by vial, 30～50 every bottle, is placed in plastic, thermal-insulation canopy or glasshouse electric heater and carries out auxiliary heating, temperature controls at 12～35 DEG C, relative humidity is 60～80%, and culture fluid proportioning is boric acid 75mg/L, vitamin B12 0mg/L, ammonium nitrate 20mg/L, and pH value is 6.0～6.5；Every 7～10d changes culture fluid.Water planting 30～40d needle-leaved bundle is taken root.
Pollen pini thunbergii water planting nursery aspect: beam Yutang etc., several factors [J] affecting Pollen pini thunbergii needle-leaved bundle isolated culture regeneration plant. Plant Physiology Communications, 1990, root media to be cultivated, young-shoot rooting rate is 1%, radical 1～3；Tao Xigang etc., Pollen pini thunbergii needle-leaved bundle Seedling Cultivation report [J]. Shandong forestry science and technology, 1985, needle-leaved bundle rooting rate is 19.3%；Zhao Guangqiu etc., the asexual reproduction method experimental study [J] of black Pinus densiflora. forestry science and technology communication, 1986, needle-leaved bundle rooting rate is 28%.
In above-mentioned technology, needle-leaved bundle is both needed to cut base and processes；Water planting carrier all adopts vial；Ammonium nitrate consumption 20-40mg/kg, biphosphate potassium application rate 20mg/kg in nutritional solution；Water planting need to carry out in plastic, thermal-insulation canopy or glasshouse；In Temperature and Humidity Control, Li Fu show temperature 15～25 DEG C, humidity more than 85%, Yang Cheng osmanthus temperature 16～28 DEG C, humidity more than 80%, the few brightness temperature 16～24 DEG C of car, permitted Asia temperature 12～35 DEG C, humidity 60～80%.
Current prior art exists following not enough:
1 cuts base not easily grasps, and needle-leaved bundle is cut when base processes and easily cut growing point, and operation is wasted time and energy；
Water planting bottle made by 2 vials, there is ventilating air permeability difference, needle-leaved bundle and easily goes mouldy and unsettled problem；
3 ammonium nitrates and biphosphate potassium application rate are low, there is needle-leaved bundle and occur yellow problem because of malnutrition；
4 low temperature are low, the temperature difference big, there is needle-leaved bundle rootage duration delay issue；In rootage duration delay situation, during high humidity, needle-leaved bundle easily goes mouldy.
Summary of the invention:
It is an object of the invention to overcome the deficiency of above-mentioned prior art, and a kind of black Pinus densiflora needle-leaved bundle water planting rooting method is provided；The problem such as mainly solve in prior art because carrier is unreasonable, Qie Ji not easily grasps, the easy yellow of needle-leaved bundle that malnutrition and humiture poor controllability etc. cause is gone mouldy, efficiency is low, technological applicability is not strong.
The technical scheme is that a kind of black Pinus densiflora needle-leaved bundle water planting rooting method, it is characterized in that, comprises the steps:
A solution culture facilities: being modulated into water planting frame with group training frame, water planting frame divides multilamellar, adopts LED illumination, and water planting place is indoor；
B water planting carrier: adopt plastic pallet as hydroponic box, thick 1.2～1.5cm cystosepiment is needle-leaved bundle carrier, and cystosepiment is cut into the fritter less than 15 × 15cm；
C needle-leaved bundle gathers: annual 1～February, from the raw black Pinus densiflora clone of 2～4a or hybrid F1For on nursery stock, the raw branch of the 1a that clip reaches full growth, gather that color is dark green, the needle-leaved bundle of robust growth is as test material；
D needle-leaved bundle sand is hidden: takes height 15 ~ 20cm Box of foamed plastics, bear, contains the thin river sand of the disinfecting solution of potassium permanganate through 0.5% into wherein；
Choose the base portion needle-leaved bundle with growing point, with the KMnO of 0.3%4Solution soaking base portion 10min, flowing water is cleaned；
Needle-leaved bundle branch after cleaning imbeds in thin river sand, and the degree of depth is the 1/3 of needle-leaved bundle length, needle-leaved bundle tilts during embedment direct motion and launches, line space 6～8cm；
Will be equipped with the Box of foamed plastics of needle-leaved bundle and put into indoor ventilated, without direct sunlight, control indoor temperature 2～8 DEG C, keep river sand humidity 50% ~ 60%, husky Tibetan time 30d；
E compounding: according to H3BO350mg·L-1+NH4NO360mg·L-1+VB125mg·L-1+KH2PO440mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be made into nutritional solution；The pH value of described nutritional solution is 6.0～6.5；
F HORMONE TREATMENT: after husky Tibetan is terminated, take out needle-leaved bundle and remove fine sand, select that leaf color is normal, base portion without the needle-leaved bundle going mouldy, expanding, steep needle-leaved bundle base portion 30min by 800 times of liquid of 70% thiophanate-methyl wettable powder or 800 times of immersions of 10% Wettable polyoxin powder；
Take out needle-leaved bundle and base portion is managed, putting into and use 200mg L-1IAA+200mg·L-1In the growth regulator of IBA preparation, soak needle-leaved bundle base portion 15h, aseptic water washing 3～4 times after immersion；
G needle-leaved bundle penetrates fixing: punches on cystosepiment with elongated hard thing, is closed up by needle-leaved bundle tip, penetrates along punching direction, just penetrates cystosepiment with sheath and be advisable；Needle-leaved bundle spacing 1 × 1.5cm；Cystosepiment with needle-leaved bundle is inserted equipped with in the plastic pallet of nutritional solution, a pallet holding multiple pieces, do not overlap each other；Depth Nutrient Solution 2.5～3.5cm；
H humiture control: in indoor, is placed in above-mentioned plastic pallet on water planting frame, and being placed in illumination is 150～180 μm of ol m-2·s-1LED under cultivate, illumination every day 12h, control temperature 23～28 DEG C, relative humidity 50～70%；Every 6～7d changes 1 nutritional solution, and timing is observed and records black Pinus densiflora needle-leaved bundle condition of rooting.
Further, the needle-leaved bundle collection described in step c, right-handed thumb and forefinger pinch needle-leaved bundle base portion, first left and right directions twisting, then pull along the needle-leaved bundle direction of growth, take off needle-leaved bundle.
Further, it is 60% ~ 70% that the Sheng described in Step d enters the thin river sand humidity of Box of foamed plastics, thin river sand from case along 2～3cm.
Compared with the prior art a kind of black Pinus densiflora needle-leaved bundle water planting rooting method of the present invention has prominent substantive distinguishing features and marked improvement:
1, needle-leaved bundle utilization rate and effect are obviously improved.This method processes without needle-leaved bundle is cut base, and in prior art, needle-leaved bundle is cut base and processed operation and waste time and energy, and easily cuts growing point；
2, change water planting carrier, solve the unsettled problem of needle-leaved bundle.Water planting bottle made by the commonly used vial of prior art, 30～50 every bottle, exists and is crowded together ventilating air permeability difference, easily goes mouldy；Needle-leaved bundle contacts inconsistent with nutritional solution, and particularly after Adventitious root initiation, it is unsettled that crowded space easily causes part needle-leaved bundle；The method of the present invention adopts plastic pallet to be needle-leaved bundle carrier as hydroponic box, cystosepiment, and ventilating air permeability is good, and needle-leaved bundle contacts uniformity with nutritional solution, solves vial and makes the problems referred to above that water planting bottle exists；
3, the improvement of ratio of nutrient solution, solves needle-leaved bundle yellow problem.Prior art Middle nutrition liquid proportioning water planting, easily occurs that yellow occurs because of malnutrition in needle-leaved bundle；And the method ratio of nutrient solution of the present invention increases ammonium nitrate and biphosphate potassium application rate water planting, needle-leaved bundle yellow is effectively solved；
4, humiture more controllability, shortens rootage duration obvious.In prior art, water planting need to set up hot house, and low temperature is low, the temperature difference is big, and rootage duration needs 30～40d mostly；And the present invention carries out in indoor, illumination is 150～180 μm of ol m-2·s-1, illumination every day 12h；Temperature 23-28 DEG C, relative humidity 50～70%；The raising of water planting temperature and the improvement of illumination, make needle-leaved bundle water planting 15～19d to take root, and after 25d, average root is up to 2.7cm；Take root in advance more than 15d than prior art.
Accompanying drawing illustrates:
Fig. 1 is embodiments of the invention 1 water planting situation of the taking root schematic diagram of 12 days；
Fig. 2 is embodiments of the invention 1 water planting situation of the taking root schematic diagram of 15 days；
Fig. 3 is embodiments of the invention 1 water planting situation of the taking root schematic diagram of 19 days；
Fig. 4 is embodiments of the invention 1 water planting situation of the taking root schematic diagram of 25 days.
Detailed description of the invention:
In order to be more fully understood that and enforcement, describe the present invention in detail below in conjunction with embodiment；Illustrated embodiment is only used for explaining the present invention, is not intended to limit the scope of the present invention.
Embodiment 1, black Pinus densiflora needle-leaved bundle water planting rooting method, adopt following steps:
1, solution culture facilities: being modulated into water planting frame with group training frame, water planting frame can divide multilamellar, adopts LED illumination；Water planting place is indoor；
2, water planting carrier: adopt plastic pallet as hydroponic box, thick 1.35cm cystosepiment is needle-leaved bundle carrier, and cystosepiment is cut into the fritter less than 15 × 15cm；
3, needle-leaved bundle collection: annual 1～February, from the raw black Pinus densiflora clone of 2～4a, the raw branch of the 1a that clip reaches full growth, gather that color is dark green, the needle-leaved bundle of robust growth is as test material；During collection, right-handed thumb and forefinger pinch needle-leaved bundle base portion, first left and right directions twisting, then pull along the needle-leaved bundle direction of growth, take off needle-leaved bundle；
4, needle-leaved bundle sand is hidden: taking height 17.5cm Box of foamed plastics, bear is several, is contained by the thin river sand (humidity 60-70%) of disinfecting solution of potassium permanganate through 0.5% into wherein, from case along 2.5cm；
Choose the base portion needle-leaved bundle with growing point, with the KMnO of 0.3%4Solution soaking base portion 10min, flowing water is cleaned；
In needle-leaved bundle branch embedment sand after cleaning, the degree of depth is about the 1/3 of needle-leaved bundle length, needle-leaved bundle slightly tilts during embedment direct motion and launches, line space 7cm；
Will be equipped with the Box of foamed plastics of needle-leaved bundle and put into indoor ventilated, without direct sunlight, control indoor temperature 2～8 DEG C, keep river sand humidity 50% ~ 60%, husky Tibetan time 30d；
5, preparation nutritional solution: according to H3BO350mg·L-1+NH4NO360mg·L-1+VB125mg·L-1+KH2PO440mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be configured to nutritional solution, the pH value of nutritional solution is 6.0～6.5；
6, HORMONE TREATMENT: husky hide terminate after, take out needle-leaved bundle and also remove fine sand, select that leaf color is normal, base portion without the needle-leaved bundle going mouldy, slightly expanding, steep needle-leaved bundle base portion 30min by 800 times of immersions of 70% thiophanate-methyl wettable powder；
Take out needle-leaved bundle and base portion is managed, putting into and use 200mg L-1IAA+200mg·L-1In the growth regulator of IBA preparation, soak needle-leaved bundle base portion 15h, aseptic water washing 3 times after immersion；
7, needle-leaved bundle penetrates fixing: punch on cystosepiment with closely-pitched label, is closed up by needle-leaved bundle tip, penetrates along punching direction, just penetrates cystosepiment with sheath and be advisable；Needle-leaved bundle spacing 1 × 1.5cm；Cystosepiment with needle-leaved bundle is inserted equipped with in the plastic pallet of nutritional solution, pallet can holding multiple pieces, be as the criterion not overlap, Depth Nutrient Solution 3.0cm；
8, humiture control: in indoor, is placed in above-mentioned plastic pallet on water planting frame, and being placed in illumination is 165 μm of ol m-2·s-1LED under cultivate, illumination every day 12h, control temperature 23～28 DEG C, relative humidity 50～70%；Every 6.5d changes 1 nutritional solution, and timing is observed and records black Pinus densiflora needle-leaved bundle condition of rooting.
In 2013～2015a, the factor such as the elite stand age that the black Pinus densiflora water planting of impact is taken root, sampling time, husky Tibetan time, Qie Ji, ratio of nutrient solution, growth regulator has been tested.
Adopt the scheme of above-described embodiment 1: referring to Fig. 1-4, after water planting 6d, cell starts division, cut surface forms callus one layer white, after 9d, needle-leaved bundle base portion forms spherical callus group, 12d rear section Callus formation white adventitious root, 15～19d major part Callus formation adventitious root, after 25d, average root is up to 2.7cm, and needle-leaved bundle rooting rate is up to 89%.The Pollen pini thunbergii water planting rooting rate obtained than Zhao Guang Qiu Hetaoxi hilllock has been respectively increased 61% and 69.7%.
Embodiment 2, black Pinus densiflora needle-leaved bundle water planting rooting method, comprise the steps:
1, solution culture facilities: being modulated into water planting frame with group training frame, water planting frame can divide multilamellar, adopts LED illumination；Water planting place is indoor；
2, water planting carrier: adopt plastic pallet as hydroponic box, thick 1.2cm cystosepiment is needle-leaved bundle carrier, and cystosepiment is cut into the fritter less than 15 × 15cm；
3, needle-leaved bundle collection: annual 1～February, from 2～4d hybrid F1For on nursery stock, the raw branch of the 1a that clip reaches full growth, gather that color is dark green, the needle-leaved bundle of robust growth is as test material；During collection, right-handed thumb and forefinger pinch needle-leaved bundle base portion, first left and right directions twisting, then pull along the needle-leaved bundle direction of growth, take off needle-leaved bundle；
4, needle-leaved bundle sand is hidden: taking height 15cm Box of foamed plastics, bear is several, is contained by the thin river sand (humidity 60-70%) of disinfecting solution of potassium permanganate through 0.5% into wherein, from case along 2cm；
Choose the base portion needle-leaved bundle with growing point, with the KMnO of 0.3%4Solution soaking base portion 10min, flowing water is cleaned；
In needle-leaved bundle branch embedment sand after cleaning, the degree of depth is about the 1/3 of needle-leaved bundle length, needle-leaved bundle slightly tilts during embedment direct motion and launches, line space 6cm；
Will be equipped with the Box of foamed plastics of needle-leaved bundle and put into indoor ventilated, without direct sunlight, control indoor temperature 2～8 DEG C, keep river sand humidity 50% ~ 60%, husky Tibetan time 30d；
5, preparation nutritional solution: according to H3BO350mg·L-1+NH4NO360mg·L-1+VB125mg·L-1+KH2PO440mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be configured to culture fluid, the pH value of nutritional solution is 6.0～6.5；
6, HORMONE TREATMENT: husky hide terminate after, take out needle-leaved bundle and also remove fine sand, select that leaf color is normal, base portion without the needle-leaved bundle going mouldy, slightly expanding, steep needle-leaved bundle base portion 30min by 800 times of immersions of 10% Wettable polyoxin powder；
Take out needle-leaved bundle and base portion is managed, putting into and use 200mg L-1IAA+200mg·L-1In the growth regulator of IBA preparation, soak needle-leaved bundle base portion 15h, aseptic water washing 3 times after immersion；
7, needle-leaved bundle penetrates fixing: punches on cystosepiment with thin wire, is closed up by needle-leaved bundle tip, penetrates along punching direction, just penetrates cystosepiment with sheath and be advisable；Needle-leaved bundle spacing 1 × 1.5cm；Cystosepiment with needle-leaved bundle is inserted equipped with in the plastic pallet of nutritional solution, pallet can holding multiple pieces, be as the criterion not overlap, Depth Nutrient Solution 2.5cm；
8, humiture control: in indoor, is placed in above-mentioned plastic pallet on water planting frame, and being placed in illumination is 150 μm of ol m-2·s-1LED under cultivate, illumination every day 12h, control temperature 23～28 DEG C, relative humidity 50～70%；Every 6d changes 1 nutritional solution, and timing is observed and records black Pinus densiflora needle-leaved bundle condition of rooting.
Embodiment 3, black Pinus densiflora needle-leaved bundle water planting rooting method, comprise the steps:
1, solution culture facilities: being modulated into water planting frame with group training frame, water planting frame can divide multilamellar, adopts LED illumination；Water planting place is indoor；
2, water planting carrier: adopt plastic pallet as hydroponic box, thick 1.5cm cystosepiment is needle-leaved bundle carrier, and cystosepiment is cut into the fritter less than 15 × 15cm；
3, needle-leaved bundle collection: annual 1～February, from the raw black Pinus densiflora clone of 2～4d, the raw branch of the 1d that clip reaches full growth, gather that color is dark green, the needle-leaved bundle of robust growth is as test material；During collection, right-handed thumb and forefinger pinch needle-leaved bundle base portion, first left and right directions twisting, then pull along the needle-leaved bundle direction of growth, take off needle-leaved bundle；
4, needle-leaved bundle sand is hidden: taking height 20cm Box of foamed plastics, bear is several, is contained by the thin river sand (humidity 60-70%) of disinfecting solution of potassium permanganate through 0.5% into wherein, from case along 3cm；
Choose the base portion needle-leaved bundle with growing point, with the KMnO of 0.3%4Solution soaking base portion 10min, flowing water is cleaned；
In needle-leaved bundle branch embedment sand after cleaning, the degree of depth is about the 1/3 of needle-leaved bundle length, needle-leaved bundle slightly tilts during embedment direct motion and launches, line space 8cm；
Will be equipped with the Box of foamed plastics of needle-leaved bundle and put into indoor ventilated, without direct sunlight, control indoor temperature 2～8 DEG C, keep river sand humidity 50% ~ 60%, husky Tibetan time 30d；
5, preparation nutritional solution: according to H3BO350mg·L-1+NH4NO360mg·L-1+VB125mg·L-1+KH2PO440mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate be dissolved in deionized water, be configured to culture fluid, the pH value of nutritional solution is 6.0～6.5；
6, HORMONE TREATMENT: husky hide terminate after, take out needle-leaved bundle and also remove fine sand, select that leaf color is normal, base portion without the needle-leaved bundle going mouldy, slightly expanding, steep needle-leaved bundle base portion 30min by 800 times of immersions of 10% Wettable polyoxin powder；
Take out needle-leaved bundle and base portion is managed, putting into and use 200mg L-1IAA+200mg·L-1In the growth regulator of IBA preparation, soak needle-leaved bundle base portion 15h, aseptic water washing 4 times after immersion；
7, needle-leaved bundle penetrates fixing: punches on cystosepiment with thin wire, is closed up by needle-leaved bundle tip, penetrates along punching direction, just penetrates cystosepiment with sheath and be advisable；Needle-leaved bundle spacing 1 × 1.5cm；Cystosepiment with needle-leaved bundle is inserted equipped with in the plastic pallet of nutritional solution, pallet can holding multiple pieces, be as the criterion not overlap, Depth Nutrient Solution 3.5cm；
8, humiture control: in indoor, is placed in above-mentioned plastic pallet on water planting frame, and being placed in illumination is 180 μm of ol m-2·s-1LED under cultivate, illumination every day 12h, control temperature 23～28 DEG C, relative humidity 50～70%；Every 7d changes 1 nutritional solution, and timing is observed and records black Pinus densiflora needle-leaved bundle condition of rooting.
Claims (3)
1. a black Pinus densiflora needle-leaved bundle water planting rooting method, it is characterised in that comprise the steps:
A solution culture facilities: being modulated into water planting frame with group training frame, water planting frame divides multilamellar, adopts LED illumination, and water planting place is indoor；
B water planting carrier: adopt plastic pallet as hydroponic box, thick 1.2～1.5cm cystosepiment is needle-leaved bundle carrier, and cystosepiment is cut into the fritter less than 15 × 15cm；
C needle-leaved bundle gathers: annual 1～February, from the raw black Pinus densiflora clone of 2～4a or hybrid F1For on nursery stock, the raw branch of the 1a that clip reaches full growth, gather that color is dark green, the needle-leaved bundle of robust growth is as test material；
D needle-leaved bundle sand is hidden: takes height 15 ~ 20cm Box of foamed plastics, bear, contains the thin river sand of the disinfecting solution of potassium permanganate through 0.5% into wherein；
Choose the base portion needle-leaved bundle with growing point, with the KMnO of 0.3%4Solution soaking base portion 10min, flowing water is cleaned；
Needle-leaved bundle branch after cleaning imbeds in thin river sand, and the degree of depth is the 1/3 of needle-leaved bundle length, needle-leaved bundle tilts during embedment direct motion and launches, line space 6～8cm；
Will be equipped with the Box of foamed plastics of needle-leaved bundle and put into indoor ventilated, without direct sunlight, control indoor temperature 2～8 DEG C, keep river sand humidity 50% ~ 60%, husky Tibetan time 30d；
E compounding: according to H3BO350mg·L-1+NH4NO360mg·L-1+VB125mg·L-1+KH2PO440mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be made into nutritional solution；The pH value of described nutritional solution is 6.0～6.5；
F HORMONE TREATMENT: after husky Tibetan is terminated, take out needle-leaved bundle and remove fine sand, select that leaf color is normal, base portion without the needle-leaved bundle going mouldy, expanding, steep needle-leaved bundle base portion 30min by 800 times of liquid of 70% thiophanate-methyl wettable powder or 800 times of immersions of 10% Wettable polyoxin powder；
Take out needle-leaved bundle and base portion is managed, putting into and use 200mg L-1IAA+200mg·L-1In the growth regulator of IBA preparation, soak needle-leaved bundle base portion 15h, aseptic water washing 3～4 times after immersion；
G needle-leaved bundle penetrates fixing: punches on cystosepiment with elongated hard thing, is closed up by needle-leaved bundle tip, penetrates along punching direction, just penetrates cystosepiment with sheath and be advisable；Needle-leaved bundle spacing 1 × 1.5cm；Cystosepiment with needle-leaved bundle is inserted equipped with in the plastic pallet of nutritional solution, a pallet holding multiple pieces, do not overlap each other；Depth Nutrient Solution 2.5～3.5cm；
H humiture control: in indoor, is placed in above-mentioned plastic pallet on water planting frame, and being placed in illumination is 150～180 μm of ol m-2·s-1LED under cultivate, illumination every day 12h, control temperature 23～28 DEG C, relative humidity 50～70%；Every 6～7d changes 1 nutritional solution, and timing is observed and records black Pinus densiflora needle-leaved bundle condition of rooting.
2. one according to claim 1 black Pinus densiflora needle-leaved bundle water planting rooting method, it is characterised in that the needle-leaved bundle collection described in step c, right-handed thumb and forefinger pinch needle-leaved bundle base portion, first left and right directions twisting, then pull along the needle-leaved bundle direction of growth, take off needle-leaved bundle.
3. one according to claim 1 black Pinus densiflora needle-leaved bundle water planting rooting method, it is characterised in that it is 60% ~ 70% that the Sheng described in Step d enters the thin river sand humidity of Box of foamed plastics, thin river sand from case along 2～3cm.
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