CN105766583B - A kind of black Japanese red pine needle-leaved bundle water planting rooting method - Google Patents
A kind of black Japanese red pine needle-leaved bundle water planting rooting method Download PDFInfo
- Publication number
- CN105766583B CN105766583B CN201610179314.1A CN201610179314A CN105766583B CN 105766583 B CN105766583 B CN 105766583B CN 201610179314 A CN201610179314 A CN 201610179314A CN 105766583 B CN105766583 B CN 105766583B
- Authority
- CN
- China
- Prior art keywords
- needle
- leaved bundle
- leaved
- bundle
- water planting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Hydroponics (AREA)
Abstract
The invention discloses a kind of black Japanese red pine needle-leaved bundle water planting rooting methods, its main feature is that, including the acquisition of solution culture facilities, water planting carrier, needle-leaved bundle, needle-leaved bundle sand storage, compounding, HORMONE TREATMENT, needle-leaved bundle penetrate fixed, humiture control；The needle-leaved bundle utilization rate and effect of method are obviously improved, and change water planting carrier, solve the problems, such as that needle-leaved bundle is hanging；The improvement of conditioning agent and ratio of nutrient solution solves the problems, such as the yellow that needle-leaved bundle occurs by malnutrition；Water planting site requirements is not tight, and humiture has more controllability, and it is apparent to shorten rootage duration.
Description
Technical field：
The present invention relates to nursery subjects in forestry technology field（Code 2202220）, specifically black Japanese red pine needle
Leaf Shu Shuipei rooting methods；For the Pinus seeds asexual propagation of seedlings such as black pine, Japanese red pine.
Background technology：
Japanese red pine is important afforestation vanguard tree seed, is one of the Main Constructive Species of warm temperate zone coastal area warm nature coniferous forest.
The existing a century of black pine introduces a fine variety history, anti-for Northern Coastal Region since its anti-pine moth, Matsumura pine scale harm obtain large-area applications
It protects a forest the dominant tree of construction.
Black Japanese red pine is to make the improved seeds that maternal, Japanese red pine makees male parent selection and breeding using black pine, have growth is fast, resistance is strong,
The big feature of genetic gain.Its 7 a gives birth to height increament beyond the black Japanese red pine F of control1Population mixture 52.75%~123.32%, it is thick raw
Long amount is 1.22~1.44 times of control than compareing big 1.65~2.70cm, even crown diameter.Suitable for being pushed away in the area of black pine growth
Wide application.
Using the degeneration-resistant of selection and breeding, fast-growing, adaptable black Japanese red pine select tree material, carries out vegetative propagation and profit is protected
With raising breeding coefficient is to realize elite germplasm kindization and construction clonal seed orchard effective way, is that eco forest systerm is built
If basis and key.
Needle-leaved bundle water planting nursery is a kind of mode of vegetative propagation, and needle-leaved bundle water planting research has had made great progress, horse
Tail pine, wet-land pine tree, Chinese pine, pinus caribaea and Chai Song achieve success, but black pine water planting is unsuccessful.
The raw pinus khasys needle-leaved bundle rooting rate highest of Li Fuxiu etc., 3 a, best nutritional liquid are 75mg/ kg H3BO3+ 20mg/
kg NH4NO3 + 20mg/ kg VB1 , indoor sand storage, temperature control is at 5~8 DEG C, 30 d of time；Qie Ji, water in vinyl house
Training, control temperature is in 15~25 DEG C, 85% or more humidity.7 d change one time of nutrition liquid.Earliest rootage duration is the 30th d, the 40th~
50 d reach peak of taking root.
Yang Chenggui etc., 2 a give birth to Needle of Slash Pine beam rooting rate highest, 20~30 d of sand storage；It is big in plastics to be packed into culture plate
Water planting in canopy, controlling temperature system is in 16~28 DEG C, 80% or more humidity.
Che Shaohui, 2 a breeder mother's tree needle-leaved bundle rooting rate highests of Chinese pine, sampling time is best with December, and rooting rate is reachable
50%.5 DEG C or so of the sand storage temperature of needle-leaved bundle, the sand storage time is best with 30~35 d;Rooting rate can be improved by cutting base.It is suitable
Nutrient solution prescription be 50mgL-1+ 28 mgL of boric acid-1+ 30 mgL of ammonium nitrate-1Vitamin B1+20 mg·L-1Di(2-ethylhexyl)phosphate
The experimental enviroment of hydrogen potassium, 16-24 DEG C of maintenance may advantageously facilitate the generation of adventitious root.20 d generate adventitious root, speed of taking root after 30 d
Rate is accelerated.
Wu Huishan etc., wet plus pine tree age are 2~3 a, base processing are cut to needle-leaved bundle, using 0.5% carbendazim solution
2.5 h at 0.6 cm of base portion or more, a concentration of 50~100mg/kg of growth regulator NAA are soaked, nutrient solution is 75mg/ kg
H3BO3 + 40mg/ kg NH4NO3 + 20mg/ kg VB1, needle-leaved bundle is put in the vial equipped with nutrient solution, and vial is placed in
It is cultivated on culturing rack in the vinyl house to be shaded with shading net, every 7 d replaces 1 nutrient solution.It will be by 28~35 d
Base bottom sends out roots.
Perhaps Asia etc., the wet-land pine tree age of tree be 8~10 a, use mass concentration for 0.2~0.5% KMnO4Solution disinfection,
Indoor sand storage and lid thin film moisturizing, temperature control is at 5~8 DEG C, 25~35 d of time；It is modulated into water planting frame with tissue culture frame, at 2 months
The middle ten days, Qie Ji are put into advance prepared root element solution, and soaking time is 12~16 h, and vial makees water culture bottle, every bottle
It 30~50, is placed on electric heater in plastic, thermal-insulation canopy or glasshouse and carries out auxiliary heating, temperature is controlled 12~35
DEG C, relative humidity is 60~80%, and culture solution proportioning is boric acid 75mg/L, vitamin B1 20mg/L, ammonium nitrate 20mg/L, pH
Value is 6.0~6.5；Every 7~10 d replaces culture solution.30~40 d needle-leaved bundles of water planting are taken root.
In terms of the nursery of black pine water planting：Beam Yutang etc., several factors for influencing black pine needle-leaved bundle cultured in vitro regeneration plant
[J] Plant Physiology Communications, 1990, it is cultivated on root media, young-shoot rooting rate is 1%, radical 1~3；Pottery tin
Hilllock etc., the Shandong black pine needle-leaved bundle Seedling Cultivation report [J] forestry science and technology, 1985, needle-leaved bundle rooting rate is 19.3%；Zhao
Wide ball etc., asexual reproduction method experimental study [J] forestry science and technologies communication of black Japanese red pine, 1986, needle-leaved bundle rooting rate is
28%。
In above-mentioned technology, needle-leaved bundle is both needed to cut base processing；Water planting carrier is all made of vial；Ammonium nitrate dosage in nutrient solution
20-40mg/ kg, biphosphate potassium application rate 20mg/ kg；Water planting need to carry out in plastic, thermal-insulation canopy or glasshouse；Humiture
In control, 15~25 DEG C of Li Fu shows temperature, 85% or more humidity, 16~28 DEG C of the osmanthus Yang Cheng temperature, 80% or more humidity, vehicle lacks brightness temperature
16~24 DEG C are spent, perhaps 12~35 DEG C of Asia temperature, humidity 60~80%.
The current prior art exists following insufficient：
1 ﹚ cuts base and is not easy to grasp, and needle-leaved bundle easily cuts growing point when cutting base processing, and operation is time-consuming and laborious；
2 ﹚ vials make water culture bottle, and there are ventilating air permeabilities, and poor, needle-leaved bundle easily goes mouldy and hanging problem；
3 ﹚ ammonium nitrate and biphosphate potassium application rate are low, there are problems that needle-leaved bundle yellow occurs because of malnutrition；
4 ﹚ low temperature are low, the temperature difference is big, and there are needle-leaved bundle rootage duration delay issues；Postpone in rootage duration, humidity
Needle-leaved bundle easily goes mouldy when big.
Invention content：
The purpose of the present invention is overcoming the shortcomings of above-mentioned prior art, and provide a kind of black Japanese red pine needle-leaved bundle water planting side of taking root
Method；Mainly solve in the prior art because carrier is unreasonable, Qie Ji is not easy to grasp, caused by malnutritive and humiture poor controllability etc.
The easy yellow of needle-leaved bundle go mouldy, the problems such as efficiency is low, technological applicability is not strong.
The technical scheme is that：A kind of black Japanese red pine needle-leaved bundle water planting rooting method, is characterized in that, including such as
Lower step：
A solution culture facilities：It is modulated into water planting frame with tissue culture frame, water planting frame is divided multilayer, illuminated using LED light, and water planting place is
It is indoor；
B water planting carriers：Using plastic pallet as hydroponic box, thick 1.2~1.5 cm cystosepiments are needle-leaved bundle carrier, foam
Plate is cut into the fritter no more than 15 × 15 cm；
C needle-leaved bundles acquire：Give birth to black Japanese red pine clone or hybrid F from 2~4 a in annual 1~2 month1For on nursery stock, clip
The 1 a life branches to reach full growth, acquisition color is dark green, needle-leaved bundle of robust growth is as test material；
D needle-leaved bundle sand storages：15 ~ 20 cm Box of foamed plastics of height are taken, bear will be through 0.5% liquor potassic permanganate
The thin river sand of disinfection is contained into wherein；
Needle-leaved bundle of the base portion with growing point is chosen, with 0.3% KMnO4Solution impregnates base portion 10min, and flowing water is cleaned；
Needle-leaved bundle branch after cleaning is embedded in thin river sand, and depth is the 1/3 of needle-leaved bundle length, and when embedment inclines needle-leaved bundle
Oblique direct motion expansion, 6~8cm of line space；
Box of foamed plastics equipped with needle-leaved bundle is put into the interior of ventilated without sunlight direct projection, control indoor temperature 2~
8 DEG C, keep river sand humidity 50% ~ 60%, sand storage time 30d；
E compoundings：According to H3BO3 50mg·L-1 + NH4NO360 mg·L-1+ VB1 25 mg·L-1 +
KH2PO4 40 mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be made into
Nutrient solution；The pH value of the nutrient solution is 6.0~6.5；
F HORMONE TREATMENTs：After sand storage, take out needle-leaved bundle and simultaneously remove fine sand, select leaf color is normal, base portion without go mouldy, it is swollen
Big needle-leaved bundle is impregnated with 800 times of liquid of 70% thiophanate-methyl wettable powder, 800 times of liquid or 10% Wettable polyoxin powder
Needle-leaved bundle base portion 30min；
It takes out needle-leaved bundle and manages base portion, be put into 200 mgL-1 IAA+200mg·L-1The growth tune that IBA is prepared
It saves in agent, impregnates 15 h of needle-leaved bundle base portion, aseptic water washing 3~4 times after immersion；
G needle-leaved bundles penetrate fixation：It is punched on cystosepiment with elongated hard object, needle-leaved bundle tip is closed up, along punching side
To penetrating, cystosepiment is just penetrated with leaf sheath to be advisable；1 × 1.5 cm of needle-leaved bundle spacing；By the cystosepiment merging with needle-leaved bundle
In plastic pallet equipped with nutrient solution, a pallet holding multiple pieces do not overlap each other；2.5~3.5 cm of Depth Nutrient Solution；
H humiture controls：Indoors, above-mentioned plastic pallet is placed on water planting frame, it is 150~180 μ to be placed in illumination
mol·m-2·s-1LED light under cultivate, 12 h of daily illumination, control 23~28 DEG C of temperature, relative humidity 50~70%；Every 6~
7 d replace 1 nutrient solution, periodically observe and record black Japanese red pine needle-leaved bundle condition of rooting.
Further, the needle-leaved bundle acquisition described in step c, right-handed thumb and index finger pinch needle-leaved bundle base portion, first left
Right direction twists, then is pulled along the needle-leaved bundle direction of growth, removes needle-leaved bundle.
Further, described in Step d contain into Box of foamed plastics thin river sand humidity be 60% ~ 70%, thin river sand is away from case
Along 2~3 cm.
Compared with the prior art a kind of black Japanese red pine needle-leaved bundle water planting rooting method of the present invention has essence outstanding
Property feature and marked improvement：
1, needle-leaved bundle utilization rate and effect are obviously improved.This method is not necessarily to carry out cutting base processing to needle-leaved bundle, and has skill
It is time-consuming and laborious to cut base processing operation for needle-leaved bundle in art, and easily cuts growing point；
2, change water planting carrier, solve the problems, such as that needle-leaved bundle is hanging.Prior art generally makees water culture bottle using vial, often
30~50, bottle exists and is crowded together that ventilating air permeability is poor, easily goes mouldy；Needle-leaved bundle contacted with nutrient solution it is inconsistent, especially not
Determine after root formed, it is hanging that crowded space easily causes part needle-leaved bundle；The present invention method using plastic pallet as hydroponic box,
Cystosepiment is needle-leaved bundle carrier, and ventilating air permeability is good, and needle-leaved bundle contacts uniformity with nutrient solution, solves vial and makees water planting
The above problem existing for bottle；
3, the improvement of ratio of nutrient solution solves the problems, such as needle-leaved bundle yellow.Ratio of nutrient solution water planting in prior art, easily goes out
There is yellow because of malnutrition in existing needle-leaved bundle；And increase ammonium nitrate and potassium dihydrogen phosphate in the method ratio of nutrient solution of the present invention
Dosage water planting, needle-leaved bundle yellow are effectively solved；
4, humiture has more controllability, and it is apparent to shorten rootage duration.Water planting need to establish vinyl house, low temperature in prior art
It is low, the temperature difference is big, rootage duration needs 30~40 d mostly；And the present invention carries out indoors, illumination is 150~180 μm of olm-2·
s-1, 12 h of daily illumination；23-28 DEG C of temperature, relative humidity 50~70%；The raising of water planting temperature and the improvement of illumination, make needle
15~19 d of Shu Shuipei can take root, and average root length reaches 2.7cm after 25 d；It takes root in advance than prior art 15 d or more.
Description of the drawings：
Fig. 1 is the embodiment of the present invention 1 water planting, 12 days situation schematic diagrams of taking root；
Fig. 2 is the embodiment of the present invention 1 water planting, 15 days situation schematic diagrams of taking root；
Fig. 3 is the embodiment of the present invention 1 water planting, 19 days situation schematic diagrams of taking root；
Fig. 4 is the embodiment of the present invention 1 water planting, 25 days situation schematic diagrams of taking root.
Specific implementation mode：
For a better understanding and implementation, with reference to embodiment, the present invention will be described in detail；Illustrated embodiment is only used for solving
The present invention is released, the scope of the present invention is not intended to limit.
Embodiment 1, black Japanese red pine needle-leaved bundle water planting rooting method, using following steps：
1, solution culture facilities：It is modulated into water planting frame with tissue culture frame, water planting frame can divide multilayer, be illuminated using LED light；Water planting place
For interior；
2, water planting carrier：Using plastic pallet as hydroponic box, thick 1.35 cm cystosepiments are needle-leaved bundle carrier, cystosepiment
It is cut into the fritter no more than 15 × 15 cm；
3, needle-leaved bundle acquires：Annual 1~2 month black Japanese red pine clone is given birth to from 2~4 a, the 1 a lifes that clip reaches full growth
Branch, acquisition color is dark green, needle-leaved bundle of robust growth is as test material；When acquisition, right-handed thumb and index finger pinch
Needle-leaved bundle base portion, first left and right directions twisting, then pulled along the needle-leaved bundle direction of growth, remove needle-leaved bundle；
4, needle-leaved bundle sand storage：17.5 cm Box of foamed plastics of height are taken, bear is several, will be through 0.5% potassium permanganate
The thin river sand of solution disinfection（Humidity 60-70%）It contains into wherein, away from 2.5 cm of case upper edge；
Needle-leaved bundle of the base portion with growing point is chosen, with 0.3% KMnO4Solution impregnates base portion 10min, and flowing water is cleaned；
Needle-leaved bundle branch after cleaning be embedded to it is husky in, depth is about the 1/3 of needle-leaved bundle length, when embedment slightly by needle-leaved bundle
Tilt direct motion expansion, 7 cm of line space；
Box of foamed plastics equipped with needle-leaved bundle is put into the interior of ventilated without sunlight direct projection, control indoor temperature 2~
8 DEG C, keep river sand humidity 50% ~ 60%, 30 d of sand storage time；
5, nutrient solution is prepared：According to H3BO3 50mg·L-1 + NH4NO360 mg·L-1+ VB1 25 mg·L-1 +
KH2PO4 40 mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, prepare
At nutrient solution, the pH value of nutrient solution is 6.0~6.5；
6, HORMONE TREATMENT：It after sand storage, takes out needle-leaved bundle and simultaneously removes fine sand, select that leaf color is normal, base portion is without going mouldy, slightly
There is the needle-leaved bundle expanded, impregnates needle-leaved bundle base portion 30min with 70% thiophanate-methyl wettable powder, 800 times of liquid；
It takes out needle-leaved bundle and manages base portion, be put into 200 mgL-1 IAA+200mg·L-1The growth tune that IBA is prepared
It saves in agent, impregnates 15 h of needle-leaved bundle base portion, aseptic water washing 3 times after immersion；
7, needle-leaved bundle penetrates fixation：It is punched on cystosepiment with closely-pitched label, needle-leaved bundle tip is closed up, worn along punching direction
Enter, cystosepiment is just penetrated with leaf sheath to be advisable；1 × 1.5 cm of needle-leaved bundle spacing；Cystosepiment merging with needle-leaved bundle is equipped with
In the plastic pallet of nutrient solution, pallet can holding multiple pieces, be subject to and do not overlap, 3.0 cm of Depth Nutrient Solution；
8, humiture control：Indoors, above-mentioned plastic pallet is placed on water planting frame, it is 165 μm of ol to be placed in illumination
m-2·s-1LED light under cultivate, 12 h of daily illumination, control 23~28 DEG C of temperature, relative humidity 50~70%；Every 6.5 d is more
1 nutrient solution is changed, black Japanese red pine needle-leaved bundle condition of rooting is periodically observed and recorded.
In 2013~2015 a, to influence elite stand age, sampling time that black Japanese red pine water planting takes root, the sand storage time, Qie Ji,
The factors such as ratio of nutrient solution, growth regulator are tested.
Using the scheme of above-described embodiment 1：Referring to Fig. 1-4, cell starts to divide after 6 d of water planting, and cut surface forms one
The callus of layer white, needle-leaved bundle base portion forms spherical callus group after 9 d, and part callus forms white after 12 d
Adventitious root, 15~19 d major part callus form adventitious root, and average root length reaches 2.7 cm, needle-leaved bundle rooting rate after 25 d
Up to 89%.61% and 69.7% has been respectively increased in the black pine water planting rooting rate obtained than the hilllocks Zhao Guang Qiu Hetaoxi.
Embodiment 2, black Japanese red pine needle-leaved bundle water planting rooting method, includes the following steps：
1, solution culture facilities：It is modulated into water planting frame with tissue culture frame, water planting frame can divide multilayer, be illuminated using LED light；Water planting place
For interior；
2, water planting carrier：Using plastic pallet as hydroponic box, thick 1.2 cm cystosepiments are needle-leaved bundle carrier, and cystosepiment is cut out
It is cut into the fritter no more than 15 × 15 cm；
3, needle-leaved bundle acquires：Annual 1~2 month, from 2~4 d hybrids F1For the 1 a lifes that on nursery stock, clip reaches full growth
Branch, acquisition color is dark green, needle-leaved bundle of robust growth is as test material；When acquisition, right-handed thumb and index finger pinch
Needle-leaved bundle base portion, first left and right directions twisting, then pulled along the needle-leaved bundle direction of growth, remove needle-leaved bundle；
4, needle-leaved bundle sand storage：15 cm Box of foamed plastics of height are taken, bear is several, will be molten through 0.5% potassium permanganate
The thin river sand of liquid disinfectant（Humidity 60-70%）It contains into wherein, away from 2 cm of case upper edge；
Needle-leaved bundle of the base portion with growing point is chosen, with 0.3% KMnO4Solution impregnates base portion 10min, and flowing water is cleaned；
Needle-leaved bundle branch after cleaning be embedded to it is husky in, depth is about the 1/3 of needle-leaved bundle length, when embedment slightly by needle-leaved bundle
Tilt direct motion expansion, 6 cm of line space；
Box of foamed plastics equipped with needle-leaved bundle is put into the interior of ventilated without sunlight direct projection, control indoor temperature 2~
8 DEG C, keep river sand humidity 50% ~ 60%, 30 d of sand storage time；
5, nutrient solution is prepared：According to H3BO3 50mg·L-1 + NH4NO360 mg·L-1+ VB1 25 mg·L-1 +
KH2PO4 40 mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, prepare
At culture solution, the pH value of nutrient solution is 6.0~6.5；
6, HORMONE TREATMENT：It after sand storage, takes out needle-leaved bundle and simultaneously removes fine sand, select that leaf color is normal, base portion is without going mouldy, slightly
There is the needle-leaved bundle expanded, impregnates needle-leaved bundle base portion 30min with 10% Wettable polyoxin powder, 800 times of liquid；
It takes out needle-leaved bundle and manages base portion, be put into 200 mgL-1 IAA+200mg·L-1The growth tune that IBA is prepared
It saves in agent, impregnates needle-leaved bundle base portion 15h, aseptic water washing 3 times after immersion；
7, needle-leaved bundle penetrates fixation：It is punched on cystosepiment with thin wire, needle-leaved bundle tip is closed up, worn along punching direction
Enter, cystosepiment is just penetrated with leaf sheath to be advisable；1 × 1.5 cm of needle-leaved bundle spacing；Cystosepiment merging with needle-leaved bundle is equipped with
In the plastic pallet of nutrient solution, pallet can holding multiple pieces, be subject to and do not overlap, 2.5 cm of Depth Nutrient Solution；
8, humiture control：Indoors, above-mentioned plastic pallet is placed on water planting frame, it is 150 μm of ol to be placed in illumination
m-2·s-1LED light under cultivate, 12 h of daily illumination, control 23~28 DEG C of temperature, relative humidity 50~70%；Every 6 d replaces 1
Secondary nutrient solution periodically observes and records black Japanese red pine needle-leaved bundle condition of rooting.
Embodiment 3, black Japanese red pine needle-leaved bundle water planting rooting method, includes the following steps：
1, solution culture facilities：It is modulated into water planting frame with tissue culture frame, water planting frame can divide multilayer, be illuminated using LED light；Water planting place
For interior；
2, water planting carrier：Using plastic pallet as hydroponic box, thick 1.5 cm cystosepiments are needle-leaved bundle carrier, and cystosepiment is cut out
It is cut into the fritter no more than 15 × 15 cm；
3, needle-leaved bundle acquires：Annual 1~2 month black Japanese red pine clone is given birth to from 2~4 d, the 1 d lifes that clip reaches full growth
Branch, acquisition color is dark green, needle-leaved bundle of robust growth is as test material；When acquisition, right-handed thumb and index finger pinch
Needle-leaved bundle base portion, first left and right directions twisting, then pulled along the needle-leaved bundle direction of growth, remove needle-leaved bundle；
4, needle-leaved bundle sand storage：20 cm Box of foamed plastics of height are taken, bear is several, will be molten through 0.5% potassium permanganate
The thin river sand of liquid disinfectant（Humidity 60-70%）It contains into wherein, away from 3 cm of case upper edge；
Needle-leaved bundle of the base portion with growing point is chosen, with 0.3% KMnO4Solution impregnates base portion 10min, and flowing water is cleaned；
Needle-leaved bundle branch after cleaning be embedded to it is husky in, depth is about the 1/3 of needle-leaved bundle length, when embedment slightly by needle-leaved bundle
Tilt direct motion expansion, 8 cm of line space；
Box of foamed plastics equipped with needle-leaved bundle is put into the interior of ventilated without sunlight direct projection, control indoor temperature 2~
8 DEG C, keep river sand humidity 50% ~ 60%, 30 d of sand storage time；
5, nutrient solution is prepared：According to H3BO3 50mg·L-1 + NH4NO360 mg·L-1+ VB1 25 mg·L-1 +
KH2PO4 40 mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate be dissolved in deionized water, prepare
At culture solution, the pH value of nutrient solution is 6.0~6.5；
6, HORMONE TREATMENT：It after sand storage, takes out needle-leaved bundle and simultaneously removes fine sand, select that leaf color is normal, base portion is without going mouldy, slightly
There is the needle-leaved bundle expanded, impregnates needle-leaved bundle base portion 30min with 10% Wettable polyoxin powder, 800 times of liquid；
It takes out needle-leaved bundle and manages base portion, be put into 200 mgL-1 IAA+200mg·L-1The growth tune that IBA is prepared
It saves in agent, impregnates 15 h of needle-leaved bundle base portion, aseptic water washing 4 times after immersion；
7, needle-leaved bundle penetrates fixation：It is punched on cystosepiment with thin wire, needle-leaved bundle tip is closed up, worn along punching direction
Enter, cystosepiment is just penetrated with leaf sheath to be advisable；1 × 1.5 cm of needle-leaved bundle spacing；Cystosepiment merging with needle-leaved bundle is equipped with
In the plastic pallet of nutrient solution, pallet can holding multiple pieces, be subject to and do not overlap, 3.5 cm of Depth Nutrient Solution；
8, humiture control：Indoors, above-mentioned plastic pallet is placed on water planting frame, it is 180 μm of ol to be placed in illumination
m-2·s-1LED light under cultivate, 12 h of daily illumination, control 23~28 DEG C of temperature, relative humidity 50~70%；Every 7 d replaces 1
Secondary nutrient solution periodically observes and records black Japanese red pine needle-leaved bundle condition of rooting.
Claims (3)
1. a kind of black Japanese red pine needle-leaved bundle water planting rooting method, which is characterized in that include the following steps：
A solution culture facilities：It is modulated into water planting frame with tissue culture frame, water planting frame is divided multilayer, illuminated using LED light, and water planting place is interior；
B water planting carriers：Using plastic pallet as hydroponic box, thick 1.2~1.5 cm cystosepiments are needle-leaved bundle carrier, and cystosepiment is cut out
It is cut into the fritter no more than 15 × 15 cm；
C needle-leaved bundles acquire：Give birth to black Japanese red pine clone or hybrid F from 2~4 a in annual 1~2 month1For on nursery stock, clip is fully sent out
The 1 a life branches educated, acquisition color is dark green, needle-leaved bundle of robust growth is as test material；
D needle-leaved bundle sand storages：15 ~ 20 cm Box of foamed plastics of height are taken, bear will be through 0.5% disinfecting solution of potassium permanganate
Thin river sand contain into wherein；
Needle-leaved bundle of the base portion with growing point is chosen, with 0.3% KMnO4Solution impregnates base portion 10min, and flowing water is cleaned；
Needle-leaved bundle branch after cleaning is embedded in thin river sand, and depth is the 1/3 of needle-leaved bundle length, and when embedment tilts needle-leaved bundle suitable
Row expansion, 6~8cm of line space；
Box of foamed plastics equipped with needle-leaved bundle is put into the interior of ventilated without sunlight direct projection, controls 2~8 DEG C of indoor temperature,
Keep river sand humidity 50% ~ 60%, sand storage time 30d；
E compoundings：According to H3BO3 50mg·L-1 + NH4NO360 mg·L-1+ VB1 25 mg·L-1 + KH2PO4 40
mg·L-1Proportioning, weigh boric acid, vitamin B1, ammonium nitrate, potassium dihydrogen phosphate, be dissolved in deionized water, be made into nutrient solution；Institute
The pH value for stating nutrient solution is 6.0~6.5；
F HORMONE TREATMENTs：It after sand storage, takes out needle-leaved bundle and simultaneously removes fine sand, select that leaf color is normal, base portion is without going mouldy, expand
Needle-leaved bundle impregnates needle with 800 times of liquid of 70% thiophanate-methyl wettable powder, 800 times of liquid or 10% Wettable polyoxin powder
Beam base portion 30min；
It takes out needle-leaved bundle and manages base portion, be put into 200 mgL-1 IAA+200mg·L-1The growth regulator that IBA is prepared
In, impregnate 15 h of needle-leaved bundle base portion, aseptic water washing 3~4 times after immersion；
G needle-leaved bundles penetrate fixation：It is punched on cystosepiment with elongated hard object, needle-leaved bundle tip is closed up, worn along punching direction
Enter, cystosepiment is just penetrated with leaf sheath to be advisable；1 × 1.5 cm of needle-leaved bundle spacing；Cystosepiment merging with needle-leaved bundle is equipped with
In the plastic pallet of nutrient solution, a pallet holding multiple pieces do not overlap each other；2.5~3.5 cm of Depth Nutrient Solution；
H humiture controls：Indoors, above-mentioned plastic pallet is placed on water planting frame, it is 150~180 μm of olm to be placed in illumination-2·s-1LED light under cultivate, 12 h of daily illumination, control 23~28 DEG C of temperature, relative humidity 50~70%；Every 6~7 d is more
1 nutrient solution is changed, black Japanese red pine needle-leaved bundle condition of rooting is periodically observed and recorded.
2. a kind of black Japanese red pine needle-leaved bundle water planting rooting method according to claim 1, which is characterized in that described in step c
Needle-leaved bundle acquires, and right-handed thumb and index finger pinch needle-leaved bundle base portion, and first left and right directions twists, then along the needle-leaved bundle direction of growth
It pulls, removes needle-leaved bundle.
3. a kind of black Japanese red pine needle-leaved bundle water planting rooting method according to claim 1, which is characterized in that described in Step d
Contain into Box of foamed plastics thin river sand humidity be 60% ~ 70%, thin river sand is away from 2~3 cm of case upper edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610179314.1A CN105766583B (en) | 2016-03-28 | 2016-03-28 | A kind of black Japanese red pine needle-leaved bundle water planting rooting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610179314.1A CN105766583B (en) | 2016-03-28 | 2016-03-28 | A kind of black Japanese red pine needle-leaved bundle water planting rooting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105766583A CN105766583A (en) | 2016-07-20 |
| CN105766583B true CN105766583B (en) | 2018-09-28 |
Family
ID=56391014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610179314.1A Expired - Fee Related CN105766583B (en) | 2016-03-28 | 2016-03-28 | A kind of black Japanese red pine needle-leaved bundle water planting rooting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105766583B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416807A (en) * | 2016-09-20 | 2017-02-22 | 山东省林业科学研究院 | Earlier-stage processing method for improving rooting rate of cutting slips of plants difficult to root |
| CN107232045A (en) * | 2017-06-30 | 2017-10-10 | 广西现代园林绿化工程种苗有限公司 | A kind of ciltivating process of copper coin grass |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102812893A (en) * | 2012-08-14 | 2012-12-12 | 广东省林业科学研究院 | Culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles |
| CN202714040U (en) * | 2012-04-20 | 2013-02-06 | 湖北省林业科学研究院 | Water culture slash pine needle-pointed leaf seedling with cutting soil |
| CN103109728A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Rapid seedling culturing method of pinus sylvestris in tube |
| CN105145305A (en) * | 2015-09-16 | 2015-12-16 | 湖北省林业科学研究院 | Water culture seedling method for slash pine big tree needle fascicles |
-
2016
- 2016-03-28 CN CN201610179314.1A patent/CN105766583B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202714040U (en) * | 2012-04-20 | 2013-02-06 | 湖北省林业科学研究院 | Water culture slash pine needle-pointed leaf seedling with cutting soil |
| CN102812893A (en) * | 2012-08-14 | 2012-12-12 | 广东省林业科学研究院 | Culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles |
| CN103109728A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Rapid seedling culturing method of pinus sylvestris in tube |
| CN105145305A (en) * | 2015-09-16 | 2015-12-16 | 湖北省林业科学研究院 | Water culture seedling method for slash pine big tree needle fascicles |
Non-Patent Citations (4)
| Title |
|---|
| 几种影响黑松针叶束离体培养再生植株的因子;梁玉堂等;《植物生理学通讯》;19901231(第3期);第31-33页 * |
| 奥地利黑松组培苗生根技术研究;王小玲等;《西北林学院学报》;20071231;第22卷(第1期);第63-66页 * |
| 黑松针叶束育苗技术试验报告;陶锡岗等;《山东林业科技》;19851231(第2期);第28-31页 * |
| 黑赤松的无性繁殖方法试验研究;赵广球等;《林业科技通讯》;19861231(第11期);第14-15页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105766583A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103733849B (en) | A kind of golden camellia old branch cuttage propagation method | |
| CN104798610B (en) | A kind of method for improving Chinese catalpa graft stock reproductive efficiency | |
| CN102498899A (en) | Method for cuttage propagation of rhododendron lapponicum | |
| CN104798670B (en) | A kind of Fraxinus velutina twig non-woven container cuttage and seedling culture method | |
| WO2017089958A1 (en) | Method for propagation of poplar tree from leaf cuttings | |
| CN102177795B (en) | Cuttage and seedling raising method for clematis hybrids | |
| CN101699956B (en) | Method for tender branch cutting propagation of Linze jujube | |
| CN102668979A (en) | Rooting culture method for poplar tissue culture seedlings | |
| CN101019492A (en) | Hetero stock grafting process for pecan | |
| CN104756738A (en) | Cutting seedling method for twigs of elm of solar greenhouse | |
| CN105075624B (en) | A kind of oil tea Water culture propagation method | |
| CN105454047A (en) | Tissue culture rapid propagation method of eucalyptus cloeziana | |
| CN102630544A (en) | Method for planting Picea koraiensis | |
| CN107197746B (en) | Breeding method of cunninghamia lanceolata field excellent resources | |
| CN107018896A (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN105766583B (en) | A kind of black Japanese red pine needle-leaved bundle water planting rooting method | |
| CN101953301B (en) | Rooting method of casuarina equisetifolia sterile test-tube seedling | |
| CN107926315A (en) | Chinese catalpa bud cutting method for culturing seedlings | |
| CN105766623B (en) | A kind of producing method for seed of capsicum cenospecies | |
| CN108012683A (en) | A kind of red autumnal leaves podocarpus blade cuttage breeding method | |
| CN105145305B (en) | A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree | |
| CN108719031A (en) | A kind of clematis Water culture rooting method | |
| CN109429755A (en) | A kind of method that lemon stock cultivates citrus seedling | |
| CN105638407A (en) | Method for acer palmatum gold thread twig cuttage | |
| CN103109728B (en) | Rapid seedling culturing method of pinus sylvestris in tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180928 Termination date: 20210328 |