CN108719031A

CN108719031A - A kind of clematis Water culture rooting method

Info

Publication number: CN108719031A
Application number: CN201810566669.5A
Authority: CN
Inventors: 李素梅; 李林芳; 王淑安; 李亚; 汪庆; 王鹏; 杨如同
Original assignee: Institute of Botany of CAS
Current assignee: Institute of Botany of CAS
Priority date: 2018-06-05
Filing date: 2018-06-05
Publication date: 2018-11-02
Anticipated expiration: 2038-06-05
Also published as: CN108719031B

Abstract

The invention discloses a kind of Water culture rooting methods of clematis, including selecting suitable clematis skewer item to be put into culture of rootage liquid, it is cultivated 6 weeks under spraying greenhouse experiment, wherein the culture of rootage liquid is the nutrient solution containing auxins hormone, after a plurality of 2-3cm new lives root system occurs in clematis branch, it is transferred in plug media and carries out seedling management.Ciltivating process proposed by the invention can allow clematis skewer Rapid Rooting, the method amount of taking root is big, root system is full sturdy, greatly enhance the cuttage planting percent of clematis, it solves the key technology difficulty of clematis cutting propagation, lays a good foundation for the industrialized implementation that clematis quickly breeds.

Description

A kind of clematis Water culture rooting method

Technical field：

The invention belongs to nutrient solution cuttage Rapid Rooting technical fields, and in particular to the Water culture rooting method of clematis.

Background technology：

Clematis（Clematis）It is a kind of climbing plant of Ranunculaceae, large flower and brilliant color, various in style, ornamental value is high, has The good reputation of " liana queen ".In recent years, the market demand of clematis increasingly goes up, and form sharp contrast therewith is clematis Seedling breeding is backward in technique, cannot be satisfied the market demand.Clematis modes of reproduction is generally begun at every the most commonly used is matrix cuttage The middle ten days and the last ten days in April in year, but its slow, growing-seedling period of taking root is up to 90 days, and clematis internode is longer in addition, carries the branch of bud point Limited amount, causes the matrix cutting propagation coefficient of clematis relatively low, and the large-scale production and market for limiting clematis supply It answers.Therefore, the Rapid Rooting problem for how solving clematis, is the key technique that clematis quickly breeds, to clematis Industrialization production be of great significance with meeting the market demand.

Invention content：

The purpose of the present invention is to provide a kind of clematis Water culture rooting methods, pass through the application of culture of rootage liquid so that Clematis cuttage branch Rapid Rooting, improves the cutting propagation coefficient of clematis.

The present invention is that implementation by the following technical programs is realized, a kind of clematis Water culture rooting method include with Lower step：

（1）The selection of branch：Annual semi-lignified branch is chosen, each branch retains a pair of of bud point, and upper cut is close to bud Point is cut flat, and lower cut is mitered into 45 degree of angles from bud point 4-5cm, retains a piece of intact leaf.

（2）Branch is disinfected：The branch cut, which is put into 1000 times of carbendazim solution, to be impregnated 2 hours, to notch into Row disinfection.

（3）Culture of rootage liquid is prepared：Prepare the KH of 2 M₂PO₄Mother liquor is added in babinet according to 1000 extension rates and is tuned into life Root culture solution, i.e. 2 mM of final concentration, add methyl α-naphthyl acetate so that final concentration of 2 mg/L of methyl α-naphthyl acetate.Culture of rootage liquid is weekly more It changes once, wherein methyl α-naphthyl acetate only adds in first week culture of rootage liquid, and the nutrient solution replaced below no longer adds.

（4）Water culture：The clematis branch cut is fixed with sponge in the circular hole for being inserted into water planting case lid, and lower cut is allowed to soak Steep 2-3 cm in culture of rootage liquid.

（5）Environmental parameter regulates and controls：Water planting babinet is placed on hot-house culture, and temperature 20-25 degree is equipped with automatic spraying device, Humidity maintains 90%, 200 μm of ol m of intensity of illumination^-2 s^-1, light application time 12 hours.

（6）Water culture seedling is colonized：Cuttage can find that lower cut nearby generates huge callus after 7 days, 15 days Occur a large amount of sturdy newborn root systems afterwards, the skewer item of 2-3 cm root longs can transplant in plug media, place in spraying greenhouse Routine Management nursery.

It is compared to the existing matrix cottage method of clematis, is taken root with nutrition using the Water culture of culture of rootage liquid State is uniform, rootage duration is short, the healthy and strong advantages such as consistent of root system, improves the survival rate of clematis cuttage and seedling culture, and can be with It is not subject to seasonal restrictions and carries out cycle cuttage root-taking, suitable for being applied in large-scale production, greatly improve clematis Cutting propagation efficiency, lay a good foundation for the industrialization production of clematis.

Specific implementation mode：

To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, the present invention is made It is further to be described in detail.

1）The configuration of mother liquor：Water culture nutrient solution is the KH of 2 mM₂PO₄, contain 2 mg/L auxins hormone naphthalenes simultaneously Acetic acid；Specifically, 136 grams of KH₂PO₄It is dissolved in the mother liquor that 1 M is configured in 1L pure water, 500 times of dilutions use；Methyl α-naphthyl acetate is used Dilute NaOH solution dissolves the mother liquor for being made into 2 mg/ml, and 1000 times of dilutions use.

2）The preparation of water planting container：Using the gray finish case of 400*600*125 mm sizes as culture babinet, clear water It cleans spare, is capped PVC board in finishing box, 6*9 circular hole is got according to 25 mm of aperture, 40 mm of spacing in PVC board.

3）Water culture clematis：The branch of annual stalwartness is chosen, each branch retains a pair of of bud point, and upper cut is close Bud point is cut flat, and lower cut is chamfer from bud point 4-5cm at 45 degree of angles, retains a piece of complete leaf, it is molten to be first put into 1000 times of carbendazim It is impregnated 2 hours in liquid, carries out disinfection to notch, then fix being put into circular hole with sponge wrapping, its lower cut is made to immerse culture 2-3 cm in liquid.It is placed in 20-25 degree spraying greenhouse and cultivates, the situation of taking root of continuous observation clematis.

4）Cuttage root-taking, the situation of taking root of each kind are carried out to clematis fallen leaves and evergreen different cultivars using the present invention It is as follows：

Kind	Time（It）	It averagely takes root number（Item）	Rooting rate（%）
				Ivan's Mancur Olson	16	6.4	95%
Unreal purple	20	5.3	90%
				Snow dance	35	5	93%

5）The result shows that being cultivated under conditions of the present embodiment, clematis fallen leaves kind Ivan Mancur Olson and unreal purple were in culture 2 weeks Start to take root afterwards, evergreen kind snow dance is slightly slow, this may it is thicker with the epidermis of evergreen kind, callus has more present incision has It closes；The rooting rate of each kind can be 90% or more, and root amount is big, root system is strong, is transferred in matrix and grows rapidly.

Above is only a specific embodiment of the present invention, not it is used for limiting the practical range of the present invention, it is all according to this Any content modification, equivalent replacement, the improvement etc. done in patent application thinking, should be included in protection scope of the present invention it It is interior.

Claims

1. a kind of Water culture rooting method of clematis, including：Suitable clematis branch is chosen, is immersed in and is taken root In culture solution, cultivated 6 weeks under greenhouse experiment of spraying；Wherein the culture of rootage liquid is the nutrition containing auxins hormone Liquid；It is transferred to plug media after newly bearing the healthy and strong flourishing root systems of 2-3 cm and carries out seedling management.

2. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：That chooses waits for skewer Item of transplanting is annual semi-lignified branch, and each branch retains a pair of of bud point, and upper cut is cut flat close to bud point, lower cut from Bud point 4-5cm is chamfer in 45 degree of angles, retains a piece of complete leaf.

3. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：The branch cut is put Enter to impregnate in 1000 times of diluted carbendazim 2 hours and carries out notch disinfection.

4. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Cutting after disinfection It is fixed in the circular hole for being inserted into water planting case lid with sponge, lower cut is made to be soaked into culture of rootage liquid.

5. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Culture of rootage liquid Major Nutrient composition be 2 mM KH₂PO₄。

6. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Culture of rootage liquid In auxins hormone be methyl α-naphthyl acetate, the mother liquor of 2 mg/ml is prepared in ethyl alcohol dissolving, and 1000 times of dilutions are added, and use final concentration For 2 mg/L.

7. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Culture of rootage liquid is every Zhou Genghuan is primary, and wherein methyl α-naphthyl acetate only adds in first week culture of rootage liquid, and the nutrient solution replaced below no longer adds.

8. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Condition of culture is Greenhouse, temperature 20-25 degree are equipped with automatic spraying device and control humidity, 200 μm of ol m of intensity of illumination^-2 s^-1。

9. a kind of Water culture rooting method of clematis according to claim 1, it is characterised in that：Each cutting is newborn It is transferred in plug media after going out the healthy and strong flourishing root systems of 2-3cm, Routine Management nursery.