CN109716940A - A kind of method of indoor quickly breeding vine tea seedling - Google Patents
A kind of method of indoor quickly breeding vine tea seedling Download PDFInfo
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- CN109716940A CN109716940A CN201910074726.2A CN201910074726A CN109716940A CN 109716940 A CN109716940 A CN 109716940A CN 201910074726 A CN201910074726 A CN 201910074726A CN 109716940 A CN109716940 A CN 109716940A
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- vine tea
- cuttage
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- tea seedling
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Abstract
The present invention relates to a kind of methods of indoor quickly breeding vine tea seedling, belong to field of plant tissue culture technique.This method chooses the vine tea branch of semi-lignified or the tendril of vine tea browning is cut into cuttage branch as raw material；Cuttage branch is dipped into rooting powder solution；It takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；Cuttage stem cutting will be dried enter water planting field planting sponge and be placed in fast numerous disk；Fast numerous disk equipped with cuttage branch is placed in the Huo Gelan nutrient solution of India pears type spore bacterium and is cultivated；It is bred in the environment for controlling humidity, temperature, until long seedling.Propagation method of the invention, reproduction speed is fast, not by seasonal effect, and reproductive survival rate is high.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and in particular to a kind of method of indoor quickly breeding vine tea seedling.
Background technique
Vine tea is traditional one of Chinese herbal medicine, civil using extensive.Vine tea is Vitaceae Ampelopsis ampelopsis grossdentata
The young stem and leaf of (Ampelopsis grossedentata) (Hand-Mazz) W.T.wang is widely distributed in the torrid zone and Asia heat
Band area, tender stem and blade are used for health tea beverage, be used to treat disease in terms of liver in Chinese medicine.Ampelopsis's
Active constituent is mainly flavonoids (flavonoids) substance ampelopsin (ampelopsin, AMP), is also dihydromyricetin
(dihydromyricetin, DHM).Flavonoids is reported with antiviral, antiallergy, anticoagulation, anti-inflammatory in recent years, antitumor
And anti-oxidation efficacy, effect potential to human health, therefore cause extensive concern.The biology and pharmacology of dihydromyricetin
Effect includes hypoglycemic, anti-oxidant and hepatoprotective effect, recent studies have shown that this flavonoids is living with anticancer to certain cancers
Property, including liver cancer, breast cancer, prostate cancer and bladder cancer.DHM or a kind of natural, have it is anti-oxidant, remove from
By performances such as bases.Hepatitis B viruses (HBV) and suppression are able to suppress to the research discovery HDM of the liver protection pharmacological action of this flavonoids at present
The proliferation of HepG2 cell processed, thus one for becoming screening liver disease therapy and health medicine and food is extremely promising next
Source.
Traditional vine tea is all, as people are to the understanding of vine tea health-care efficacy, to drink rattan based on the picking of wild vine tea
The number of tea is more and more, becomes larger to the demand of vine tea, and the need of people masses are only no longer satisfied by the wild vine tea of tradition
It asks, the method for this invention, a large amount of quickly breeding vine tea seedlings can be carried out, the proliferative speed of vine tea seedling is improved, increase vine tea
Seedling survives quantity, expands vine tea cultivated area, vine tea yield is improved, to meet society need.
Summary of the invention
It is an object of the invention to be mainly derived from wild species picking, limited amount, by certain for traditional vine tea
Limitation；The vine tea seedling in cultivation is mainly by based on cutting propagation in ground at present, by limitation, the present invention such as season, survival rate be low
A kind of method of indoor quickly breeding vine tea seedling is provided.This method reproduction speed is fast, high survival rate, is not subject to seasonal restrictions.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of method of indoor quickly breeding vine tea seedling of the invention, comprising the following steps:
(1) the vine tea branch of semi-lignified or the tendril of vine tea browning are chosen as raw material, and is cut into every section of 15-
20cm long, there are the cuttage branches of 2-4 section, put down on clip oblique, and upper clip is above the bud section at 1-1.5cm；
(2) the above part 3-4cm of clip under cuttage branch is dipped into rooting powder solution；
(3) it takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；
It (4) will be in the lower clip insertion water planting field planting sponge for the cuttage branch that dried；
(5) water planting for having cuttage branch field planting sponge is placed in fast numerous disk of the Huo Gelan nutrient solution equipped with India pears type spore bacterium and is trained
It supports；
(6) humid control is bred in 26-30 DEG C of environment in 80%-90%, temperature control, until long seedling.
The mass percent concentration of rooting powder solution is 50% in above-mentioned steps (2), soaking time 1h.
The formula of the Huo Gelan nutrient solution of India pears type spore bacterium in above-mentioned steps (5) are as follows:
The preparation of India's pyriform spore bacterium stoste: in activated India's pyriform spore bacterium fungus block access Huo Gelan fluid nutrient medium,
28 DEG C, 200r/min culture 7d after, obtain India's pyriform spore mycelium stoste；Mycelium stoste is diluted 10 times, is prepared
The Huo Gelan nutrient solution of India pears type spore bacterium, for the fast numerous of cuttage branch.
The wherein formula of Huo Gelan nutrient solution are as follows:
1) a great number of elements ingredient:
Four water-calcium nitrate 945mg/L
Potassium nitrate 506mg/L
Ammonium nitrate 80mg/L
Potassium dihydrogen phosphate 136mg/L
Magnesium sulfate 493mg/L
2) iron salt solutions
Ferrous sulfate heptahydrate 2.78g
Disodium ethylene diamine tetraacetate (EDTANa2) 3.73g
Distilled water 500mL
3) liquid microelement
Potassium iodide 0.83mg/l
Boric acid 6.2mg/L
Manganese sulfate 22.3mg/L
Zinc sulfate 8.6mg/L
Sodium molybdate 0.25mg/L
Copper sulphate 0.025mg/L
Cobalt chloride 0.025mg/L
Iron salt solutions 2.5mL
Liquid microelement 5mL
A great number of elements ingredient is added, distilled water is dissolved to 1L, adjusts pH=6.0.
The present invention has the advantages that
The present invention obtains cuttage branch using the tendril of vine tea branch or the vine tea browning of semi-lignified as raw material, with life
Root powder promotes skewer Cutting rooting, using a kind of Huo Gelan nutrient solution of the India pears type spore bacterium of indoor quickly breeding vine tea seedling as cuttage
The growth of branch provides nutriment, realizes the quick breeding without vine tea seedling under the conditions of soil.It can make vine tea seedling using this method
Survival rate is 1.62 times (spring survival rate is 60%) of cuttage shoot survival percent in spring ground, is cuttage seeding in summer ground up to 97%
2.16 times (summer survival rates be 45%) of survival rate are that (autumn survival rate is for 1.83 times of cuttage shoot survival percent in autumn ground
It 53%), is 2.77 times (winter survival rate is 35%) of cuttage shoot survival percent in winter ground.This method reproduction speed is fast, not by season
Section influences, and reproductive survival rate is high, grasps simple and efficient, cost savings.
Detailed description of the invention
Fig. 1 numerous cuttage branch fastly.
Fig. 2 cuttage branch is numerous fastly in fast numerous disk.
Fig. 3 spring it is fast it is numerous survive after vine tea seedling.
Fig. 4 summer it is fast it is numerous survive after vine tea seedling.
Fig. 5 summer it is fast it is numerous survive after vine tea seedling be transplanted to nutrient bag.
Fig. 6 autumn it is fast it is numerous survive after vine tea seedling.
Fig. 7 autumn it is fast it is numerous survive after vine tea seedling be transplanted to nutrient bag.
Fig. 8 winter it is fast it is numerous survive after vine tea seedling be transplanted to nutrient bag.
Specific embodiment
Embodiment 1
The present embodiment is implemented in spring
A kind of method of indoor quickly breeding vine tea seedling, comprising the following steps:
(1) the vine tea branch of semi-lignified is chosen as raw material, and is cut into every section of 15cm long, the cuttage branch there are 2 sections, clip
On put down oblique, for upper clip above the bud section at 1cm, fast numerous cuttage branch is shown in Fig. 1；
(2) the above part 3cm of clip under cuttage branch is dipped into 1h in the rooting powder solution that mass percent concentration is 50%；
(3) it takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；
(4) clip insertion water planting under the cuttage branch dried is colonized in sponge；
(5) water planting for having cuttage branch field planting sponge is placed in fast numerous disk of the Huo Gelan nutrient solution equipped with India pears type spore bacterium and is trained
It supports (Fig. 2)；
(6) bred in 26 DEG C of environment in control humidity in 80%, temperature, until long seedling, planting percent can up to 97%, it is fast it is numerous at
Vine tea seedling after work is shown in Fig. 3.
Comparative example 1
The method of spring crop field breeding vine tea seedling, comprising the following steps:
(1) the vine tea branch of semi-lignified is chosen as raw material, and is cut into every section 15 length, the cuttage branch there are 2 sections, on clip
Put down oblique, upper clip is above the bud section at 1cm；
(2) seedbed: every 667 ㎡ spreads fertilizer over the fields in seedbed high-quality farmyard manure 2500kg, deep ploughing it is thin it is whole after be made into the compartment of 1.2m wide, compartment ditch depth
40cm, finally by black film mulching in compartment face；
(3) cuttage: being that 10cm specification is punched on the film of compartment with punch by seeding row spacing, under at random inserting raw material, uses soil pressure
Real hole guarantees that aerial part has 2 minor matters；
(4) daily management: seedbed is kept, without weeds, to take sunshade net without ponding；
(5) planting percent: 60%.
The method that vine tea seedling is quickly bred in Room 1 of the embodiment of the present invention, the survival rate of vine tea seedling is up to 97%；1 spring of comparative example
The method that vine tea seedling is bred in crop field, the survival rate of vine tea seedling is up to 60%；The survival rate of 1 vine tea seedling of the embodiment of the present invention is comparative example 1
1.62 times of the survival rate of vine tea seedling.
Embodiment 2
The present embodiment is implemented in summer.
A kind of method of indoor quickly breeding vine tea seedling, comprising the following steps:
(1) tendril of vine tea browning is chosen as raw material, and is cut into every section of 20cm long, the cuttage branch there are 4 sections, clip
On put down oblique, upper sheared edge is above the bud section at 1.5cm；
(2) the above part 4cm of clip under cuttage branch is dipped into 1h in the rooting powder solution that mass percent concentration is 50%；
(3) it takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；
(4) clip insertion water planting under the cuttage branch dried is colonized in sponge；
(5) water planting for having cuttage branch field planting sponge is placed in fast numerous disk of the Huo Gelan nutrient solution equipped with India pears type spore bacterium and is trained
It supports；
(6) bred in 30 DEG C of environment in control humidity in 90%, temperature, until long seedling, planting percent can up to 97%, it is fast it is numerous at
Vine tea seedling after work is shown in Fig. 4.
It is fast it is numerous survive after vine tea seedling be transplanted to continued growth in nutrient bag (Fig. 5).
Comparative example 2
The method of summer crop field breeding vine tea seedling, comprising the following steps:
(1) tendril of vine tea browning is chosen as raw material, and is cut into every section of 20cm long, the cuttage branch there are 4 sections, clip
On put down oblique, upper clip is above the bud section at 1.5cm；
(2) seedbed: every 667 ㎡ spreads fertilizer over the fields in seedbed high-quality farmyard manure 2500kg, deep ploughing it is thin it is whole after be made into the compartment of 1.2m wide, compartment ditch depth
40cm, finally by black film mulching in compartment face
(3) cuttage: being that 10cm specification is punched on the film of compartment with punch by seeding row spacing, under at random inserting raw material, uses soil pressure
Real hole guarantees that aerial part has 4 minor matters；
(4) daily management: seedbed is kept, without weeds, to take sunshade net without ponding.
(5) planting percent: 45%.
The method that vine tea seedling is quickly bred in Room 2 of the embodiment of the present invention, the survival rate of vine tea seedling is up to 97%；2 summer of comparative example
The method that vine tea seedling is bred in crop field, the survival rate of vine tea seedling is up to 45%；The survival rate of 2 vine tea seedling of the embodiment of the present invention is comparative example 2
2.16 times of the survival rate of vine tea seedling.
Embodiment 3
The present embodiment is implemented in the fall.
A kind of method of indoor quickly breeding vine tea seedling, comprising the following steps:
(1) the vine tea branch of semi-lignified is chosen as raw material, and is cut into every section of 18cm long, the cuttage branch there are 3 sections, clip
On put down oblique, upper clip is above the bud section at about 1.3cm；
(2) the above part 3.5cm of clip under cuttage branch is dipped into 1h in the rooting powder solution that mass percent concentration is 50%；
(3) it takes out and is soaked with that root-inducing powder cuttage branch is In Shade to be dried to surface no moisture；
(4) clip insertion water planting under the cuttage branch dried is colonized in sponge；
(5) water planting for having cuttage branch field planting sponge is placed in fast numerous disk of the Huo Gelan nutrient solution equipped with India pears type spore bacterium and is trained
It supports；
(6) bred in 28 DEG C of environment in control humidity in 85%, temperature, until long seedling, planting percent can up to 97%, it is fast it is numerous at
Vine tea seedling after work is shown in Fig. 6.
It is fast it is numerous survive after vine tea seedling be transplanted to continued growth in nutrient bag (Fig. 7).
Comparative example 3
The method of autumn crop field breeding vine tea seedling, comprising the following steps:
(1) raw material that the vine tea branch of semi-lignified is used as are chosen, and are cut into every section of 18cm long, the cuttage branch there are 3 sections, are cut
Put down oblique on mouthful, upper clip is above the bud section at 1.3cm；
(2) seedbed: every 667 ㎡ spreads fertilizer over the fields in seedbed high-quality farmyard manure 2500kg, deep ploughing it is thin it is whole after be made into the compartment of 1.2m wide, compartment ditch depth
40cm, finally by black film mulching in compartment face；
(3) cuttage: being that 10cm specification is punched on the film of compartment with punch by seeding row spacing, under at random inserting raw material, uses soil pressure
Real hole guarantees that aerial part has 3 minor matters；
(4) daily management: seedbed is kept, without weeds, to take sunshade net without ponding；
(5) planting percent: 53%.
The method that vine tea seedling is quickly bred in Room 3 of the embodiment of the present invention, the survival rate of vine tea seedling is up to 97%；3 autumn of comparative example
The method that vine tea seedling is bred in crop field, the survival rate of vine tea seedling is up to 53%；The survival rate of 3 vine tea seedling of the embodiment of the present invention is comparative example 3
1.83 times of the survival rate of vine tea seedling.
Embodiment 4
The present embodiment is implemented in winter.
A kind of method of indoor quickly breeding vine tea seedling, comprising the following steps:
(1) the vine tea branch raw material for choosing semi-lignified are cut into every section of 20cm long, the cuttage branch there are 4 sections, under putting down on clip
Tiltedly, upper clip is above the bud section at 1.5cm；
(2) the above part 4cm of clip under cuttage branch is dipped into 1h in the rooting powder solution that quality is 50% than percent concentration；
(3) it takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；
(4) clip insertion water planting under the cuttage branch dried is colonized in sponge；
(5) it will be cultivated in the fast numerous disk for the Huo Gelan nutrient solution that the water planting for having cuttage branch field planting sponge is placed in India pears type spore bacterium；
(6) it is bred in 26 DEG C of environment in control humidity in 80%, temperature, until long seedling, planting percent can be up to 97%.
It is fast it is numerous survive after vine tea seedling be transplanted to continued growth in nutrient bag (Fig. 8).
Comparative example 4
The method of winter crop field breeding vine tea seedling, comprising the following steps:
(1) the vine tea branch of semi-lignified is chosen as raw material, and is cut into every section of 20cm long, the cuttage branch there are 4 sections, clip
On put down oblique, upper clip is above the bud section at 1.5cm；
(2) seedbed: every 667 ㎡ spreads fertilizer over the fields in seedbed high-quality farmyard manure 2500kg, deep ploughing it is thin it is whole after be made into the compartment of 1.2m wide, compartment ditch depth
40cm, finally by black film mulching in compartment face；
(3) cuttage: being that 10cm specification is punched on the film of compartment with punch by seeding row spacing, under at random inserting raw material, uses soil pressure
Real hole guarantees that aerial part has 4 minor matters；
(4) daily management: seedbed is kept, without weeds, to take sunshade net without ponding.
(5) planting percent: 35%.
The method that vine tea seedling is quickly bred in Room 4 of the embodiment of the present invention, the survival rate of vine tea seedling is up to 97%；4 winter of comparative example
The method that vine tea seedling is bred in crop field, the survival rate of vine tea seedling is up to 35%；The survival rate of 3 vine tea seedling of the embodiment of the present invention is comparative example 3
2.77 times of the survival rate of vine tea seedling.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (4)
1. a kind of method of indoor quickly breeding vine tea seedling, which comprises the following steps:
(1) it chooses the vine tea branch of semi-lignified or the tendril of vine tea browning is cut into cuttage branch as raw material；
(2) cuttage branch is dipped into rooting powder solution；
(3) it takes out and is soaked with the cuttage branch of root-inducing powder and In Shade dries to surface no moisture；
(4) cuttage stem cutting will be dried to enter in water planting field planting sponge；
(5) water planting for having cuttage branch field planting sponge is placed in fast numerous disk of the Huo Gelan nutrient solution equipped with India pears type spore bacterium and is trained
It supports；
(6) it is bred in the environment for controlling humidity, temperature, until long seedling.
2. a kind of method of indoor quickly breeding vine tea seedling according to claim 1, which is characterized in that step (1) is described
Cuttage every section of 15-20cm long of branch puts down oblique there are 2-4 section on clip, upper clip is above the bud section at about 1-1.5cm.
3. a kind of method of indoor quickly breeding vine tea seedling according to claim 1, which is characterized in that institute in step (2)
The mass percent concentration for the rooting powder solution stated is 50%；The above part 3-4cm of clip is dipped into rooting powder solution under cuttage branch
In；Soaking time is 1 hour.
4. a kind of method of indoor quickly breeding vine tea seedling according to claim 1, which is characterized in that institute in step (6)
The humid control for stating environment is controlled in 80%-90% temperature at 26-30 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521568A (en) * | 2019-10-09 | 2019-12-03 | 北京中农富通园艺有限公司 | A kind of rapid propagation method of wedding gauze kerchief bracketplant |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1390442A (en) * | 2001-10-26 | 2003-01-15 | 衡东县农业局 | Technique for cutting reproduction and directional transplant of vine tea |
| CN104303969A (en) * | 2014-09-28 | 2015-01-28 | 临沂大学 | Chinese rose water planting cutting propagation method |
| CN105754880A (en) * | 2016-04-19 | 2016-07-13 | 福建农林大学 | Nutrient solution for hydroponics plants capable of continuously releasing negative ions |
| CN105766458A (en) * | 2016-03-14 | 2016-07-20 | 湖北华饴生物科技有限公司 | Ampelopsin grossedentata light matrix seedling tray cuttage seedling method |
| CN108124749A (en) * | 2017-12-12 | 2018-06-08 | 浙江省亚热带作物研究所 | A kind of method that clematis Water culture is taken root |
| CN108719031A (en) * | 2018-06-05 | 2018-11-02 | 江苏省中国科学院植物研究所 | A kind of clematis Water culture rooting method |
-
2019
- 2019-01-25 CN CN201910074726.2A patent/CN109716940A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1390442A (en) * | 2001-10-26 | 2003-01-15 | 衡东县农业局 | Technique for cutting reproduction and directional transplant of vine tea |
| CN104303969A (en) * | 2014-09-28 | 2015-01-28 | 临沂大学 | Chinese rose water planting cutting propagation method |
| CN105766458A (en) * | 2016-03-14 | 2016-07-20 | 湖北华饴生物科技有限公司 | Ampelopsin grossedentata light matrix seedling tray cuttage seedling method |
| CN105754880A (en) * | 2016-04-19 | 2016-07-13 | 福建农林大学 | Nutrient solution for hydroponics plants capable of continuously releasing negative ions |
| CN108124749A (en) * | 2017-12-12 | 2018-06-08 | 浙江省亚热带作物研究所 | A kind of method that clematis Water culture is taken root |
| CN108719031A (en) * | 2018-06-05 | 2018-11-02 | 江苏省中国科学院植物研究所 | A kind of clematis Water culture rooting method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521568A (en) * | 2019-10-09 | 2019-12-03 | 北京中农富通园艺有限公司 | A kind of rapid propagation method of wedding gauze kerchief bracketplant |
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Application publication date: 20190507 |