CN108124749A - A kind of method that clematis Water culture is taken root - Google Patents

A kind of method that clematis Water culture is taken root Download PDF

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Publication number
CN108124749A
CN108124749A CN201711320853.3A CN201711320853A CN108124749A CN 108124749 A CN108124749 A CN 108124749A CN 201711320853 A CN201711320853 A CN 201711320853A CN 108124749 A CN108124749 A CN 108124749A
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clematis
cuttage
water culture
cuttings
water
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CN108124749B (en
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胡青荻
郑坚
钱仁卷
张旭乐
马晓华
刘洪见
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ZHEJIANG PROV INST OF SUBTROPICAL CROPS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

A kind of method that clematis Water culture is taken root, management, acclimatization and transplants process after the preparation of preparation, mill water culture nutrient solution including cuttage vessel, the selection of clematis cuttings and processing, water planting culture, cuttage, on the basis of water culture technique, by influence of the plant growth regulator to clematis cuttage root-taking, cutting plantation is improved;Make the conditions such as moisture, temperature, illumination and air humidity controllable during cutting propagation, reduce influence of the external environment to plant propagation rate, cutting plantation is stablized, plant growth state well overcomes the problems, such as that cuttage is subject to seasonal restrictions, shorten breeding cycle, improve cuttage survival rate, cuttings survival rate is up to more than 85%, be conducive to factorial praluction and the popularization and application of clematis plant, there are preferable economic benefit, social benefit and ecological benefits.

Description

A kind of method that clematis Water culture is taken root
Technical field
The invention belongs to plant cuttage propagation field, a kind of method taken root more particularly to clematis Water culture.
Background technology
Clematis(Clematis L.)It is under the jurisdiction of Ranunculaceae(Ranunculaceae), it is liana the most popular in recent years One of flowers, pattern enriches, flower pattern is different, type is various, possesses beautiful, graceful, generous unique ornamental value, can not only be used for The pot flowers of novel in style, it can also be used to the ground such as wall, arched door, style system vertical afforest, can also make cut-flower, by etc. should With leading in many world-class flower shows, " tough such as iron wire, the flowers are in blossom such as lotus " has the laudatory title of " liana queen ".In recent years, With flourishing for home gardening and afforestation, market demand also increases year by year, and only Zhejiang rainbow in 2016 is more limited The clematis sales volume of company increases to 5,000,000 from 800,000 in 2010, favorite of the clematis as home gardening, it is deep by The favorite outstanding person of consumer.
As having green plants with broad prospects for development, market demand gradually increases clematis, main at this stage There are three types of modess of reproduction:One kind is seed propagation, with the full seed of current year picking, is sowed in the fall, but clematis seed is sprouted Hair rate is relatively low, and most of clematis seed needs to sprout by the low temperature period of vernalization, and germinating time is longer, can not stablize Large-scale breeding;Second is cutting propagation, and the prior art generally carries out in the fall, needs can just take root, and skewer within 1-2 months It is unstable to be inserted into motility rate, is affected by the external environment larger, it can not production breeding;The third is mound layering, and this method uses Last year generates ripe branch press strip, usually takes root in 1 year, takes longer.
In general, the clematis seeding and seedling raising period is longer, and seedling quantity is unstable, in production to take cutting propagation more Technology, but also due to cuttage survival rate is relatively low, it is slow-growing, influence the popularization of its large-scale breeding.Therefore, how iron wire is solved Lotus cuttage difficult problem is the key that optimization clematis quickly breeds, and breeding, which is factory produced, to clematis is of great significance.
Invention content
In order to overcome the shortcomings of background technology, the present invention provides a kind of clematis Water culture rooting method, improves existing Cultivation period is long, it is slow to take root, survival rate is unstable present situation.
The technical solution adopted in the present invention:A kind of clematis Water culture rooting method, including following operating procedure:
1)The preparation of cuttage vessel:Opaque babinet is selected as cuttage vessel;
2)The preparation of mill water culture nutrient solution:Needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B and inorganic chelator EDTA-Na2, and pH value is 5.0-5.5;
3)The selection and processing of clematis cuttings:Select the maturation zone bud branch of the no disease and pests harm of semi-lignified or lignifying as Cuttings, the long 3-4cm of cuttings, top section are cross section, and lower part section is scarf, 1 blade is only stayed, by cuttings base portion 1- 2cm submerges and 15min is impregnated in plant growth regulator, and processing of drying in the shade is taken out after immersion, wherein, the plant growth regulator Select the methyl α-naphthyl acetate of a concentration of 200mg/L(NAA)Or the indolebutyric acid of a concentration of 100mg/L(IBA);
4)Water planting culture:Mill water culture nutrient solution is filled it up in cuttage vessel, by the water suction sea of the cuttings obtained in step 3 2cm thickness Continuous item package, and pass through cuttage fixed plate fixed placement in cuttage vessel;
5)It is managed after cuttage:Water planting room temperature is 18-28 DEG C, humidity 80-100%, and nutrient solution is replaced in timing, is cultivated 1-4 weeks;
6)Acclimatization and transplants:When the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, after transplanting Pour 1 water daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling.
Nutrient is with densimeter, N in the mill water culture nutrient solution:1.25-2.0 mM/L、P:0.15-0.2 mM/L、K: 0.4-0.8 mM/L、Ca:0.4-1.0 mM/L、Mg:0.4-1.0 mM/L、Fe:10-15 mM/L、Mn:3-6 mM/L、Mo: 0.1-0.35 mM/L、B:10-18 mM/L、Zn:0.3-0.7 mM/L、Cu:0.15-0.3 mM/L, inorganic chelator EDTA- A concentration of 20-40 mM/L of Na2, and pass through and add in HCl solution progress pH value adjusting.
The cuttage vessel is width 20-30cm, the plastic box of long 30-40cm, depth 10-15cm, the cuttage fixed plate For wide 20-30cm, long 30-40cm, thickness 0.8-1.5cm PVC foam plate, size just covers cuttage vessel, the cuttage Fixed plate is equipped with the circular hole of a diameter of 2.5cm, and mouth is placed as cuttings.
The cuttage fixed plate is carried out disinfection with 30%NaClO, is cleaned after impregnating 20min.
Mill water culture nutrient solution is replaced once, and mill water culture nutrient solution is kept to be contacted with water-absorbing sponge item, while avoid sunlight for every 3 days Direct projection keeps room ventilation good, and water planting is until take root.
The seedling cultivation depth is advisable with covering new root just, seedling medium for peat and perlite uniformly mix and Into mixed proportion 1:1.
The beneficial effects of the invention are as follows:Using above scheme, 1, the present invention on the basis of water culture technique, given birth to by plant Influence of the long conditioning agent to clematis cuttage root-taking improves cutting plantation;Make moisture, temperature, light during cutting propagation According to controllable with the conditions such as air humidity, influence of the external environment to plant propagation rate is reduced, cutting plantation is stablized, plant life Long status is good;2nd, the present invention can expand breeding area, while overcome the problems, such as that cuttage is subject to seasonal restrictions, and shorten breeding cycle, Cuttage survival rate is improved, cuttings survival rate is conducive to factorial praluction and the popularization and application of clematis plant, has up to more than 85% There are preferable economic benefit, social benefit and ecological benefits.
Specific embodiment
Embodiment one
A kind of clematis Water culture rooting method, including following operating procedure:
1)The preparation of cuttage vessel:Select opaque babinet as cuttage vessel and PVC foam plate as cuttage fixed plate, institute The circular hole that cuttage fixed plate is equipped with a diameter of 2.5cm is stated, mouth is placed as cuttings;
2)The preparation of mill water culture nutrient solution:Needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B and inorganic chelator EDTA-Na2, and pass through and add in HCl solution and adjust pH value to 5.0-5.5;
Wherein, nutrient presses densimeter N:1.25 mM/L、P:0.2 mM/L、K:0.5 mM/L、Ca:0.5 mM/L、Mg:0.5 mM/L、Fe:10mM/L、Mn:4mM/L、Mo:0.2mM/L、B:10mM/L、Zn:0.3 mM/L、Cu:0.1mM/L, inorganic chelator A concentration of 20mM/L of EDTA-Na2;
3)The selection and processing of clematis cuttings:Select the maturation zone bud branch of the no disease and pests harm of semi-lignified or lignifying as Cuttings, the long 3-4cm of cuttings, top section are cross section, and lower part section is scarf, 1 blade is only stayed, by cuttings base portion 1- 2cm submerges in the naphthalene acid solution of a concentration of 100mg/L and impregnates 15min, then takes out and dries in the shade to ensure to absorb;
4)Water planting culture:Mill water culture nutrient solution is filled it up in cuttage vessel, by the water suction sea of the cuttings obtained in step 3 2cm thickness Continuous item package, and the circular hole for passing through cuttage fixed plate is fixed, and cuttings is made to be immersed in mill water culture nutrient solution, water-absorbing sponge then with Mill water culture nutrient solution keeps contact;
5)It is managed after cuttage:Water planting room temperature is 18-28 DEG C, and humidity 80-100% replaces a mill water culture nutrient solution in every 3 days, together When avoid direct sunlight, keep room ventilation good, cultivate 1-4 weeks;
6)Acclimatization and transplants:When the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, after transplanting Pour 1 water daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling.
Embodiment two
A kind of clematis Water culture rooting method, operating procedure is substantially identical with embodiment one, the difference lies in:Step 3 In the middle naphthalene acid solution that cuttings base portion 1-2cm is submerged to a concentration of 200mg/L and impregnate 15min.
Embodiment three
A kind of clematis Water culture rooting method, operating procedure is substantially identical with embodiment one, the difference lies in:Step 3 In the middle naphthalene acid solution that cuttings base portion 1-2cm is submerged to a concentration of 300mg/L and impregnate 15min.
Example IV
A kind of clematis Water culture rooting method, operating procedure is substantially identical with embodiment one, the difference lies in:Step 3 In the middle indolebutyric acid solution that cuttings base portion 1-2cm is submerged to a concentration of 100mg/L and impregnate 15min.
Embodiment five
A kind of clematis Water culture rooting method, operating procedure is substantially identical with embodiment one, the difference lies in:Step 3 In the middle indolebutyric acid solution that cuttings base portion 1-2cm is submerged to a concentration of 200mg/L and impregnate 15min.
Embodiment six
A kind of clematis Water culture rooting method, operating procedure is substantially identical with embodiment one, the difference lies in:Step 3 In the middle indolebutyric acid solution that cuttings base portion 1-2cm is submerged to a concentration of 300mg/L and impregnate 15min.
Water culture is carried out by six kinds of different implementations to clematis to take root, and Statistical Comparison is as a result, obtain as follows Conclusion:
With the best results of 200mg/L naphthalene acid solutions and 100mg/L indolebutyric acid solution, through statistics, cuttage root-taking into Power is respectively 89.67% and 86.43%, is above embodiment, and quantity of taking root is 2-3 times of other groups, average root long 1.65cm, and by hardening 5 days after, reach root system stable state, reach seedling cultivation requirement.
When seedling is cultivated, seedling cultivation depth is advisable with covering new root just, and seedling medium is uniform for peat and perlite It mixes, mixed proportion 1:1.
In addition, when preparing mill water culture nutrient solution, each the concentrating of nutrients allows there are certain error, the mill water culture nutrient solution Middle nutrient, inorganic chelator EDTA-Na2 are maintained at densimeter in following range:N:1.25-2.0 mM/L、P: 0.15-0.2 mM/L、K:0.4-0.8 mM/L、Ca:0.4-1.0 mM/L、Mg:0.4-1.0 mM/L、Fe:10-15 mM/L、 Mn:3-6 mM/L、Mo:0.1-0.35 mM/L、B:10-18 mM/L、Zn:0.3-0.7 mM/L、Cu:0.15-0.3 mM/L, nothing Machine chelating agent EDTA-Na2:20-40 mM/L.
The size of the cuttage vessel is just all right suitable for cuttings, should not be too large wasting space, also unsuitable too small Growth is influenced, cuttage vessel generally selects the plastic box of wide 20-30cm, long 30-40cm, depth 10-15cm, and the cuttage is consolidated Fixed board size just covers cuttage vessel, width 20-30cm, long 30-40cm, thickness 0.8-1.5cm, the cuttage fixed plate Before the use, can 20min be impregnated by 30%NaClO, carry out disinfection cleaning, it is ensured that nontoxic sterile to clematis cuttings one Good growing environment, be further ensured that survival rooting rate.
Embodiment is not construed as the limitation to invention, but any spiritual improvements introduced based on the present invention, all Ying Ben Within the protection domain of invention.

Claims (7)

1. a kind of method that clematis Water culture is taken root, it is characterised in that:Including following operating procedure:
1)The preparation of cuttage vessel:Opaque babinet is selected as cuttage vessel;
2)The preparation of mill water culture nutrient solution:Needed in the mill water culture nutrient solution comprising nutrient N, P, K, Ca, Mg, Fe, Zn, Mn, Cu, Mo, B and inorganic chelator EDTA-Na2, and pH value is 5.0-5.5;
3)The selection and processing of clematis cuttings:Select the maturation zone bud branch of the no disease and pests harm of semi-lignified or lignifying as Cuttings, the long 3-4cm of cuttings, top section are cross section, and lower part section is scarf, 1 blade is only stayed, by cuttings base portion 1- 2cm submerges and 15min is impregnated in plant growth regulator, and processing of drying in the shade is taken out after immersion;
4)Water planting culture:Mill water culture nutrient solution is filled it up in cuttage vessel, by the water suction sea of the cuttings obtained in step 3 2cm thickness Continuous item package, and pass through cuttage fixed plate fixed placement in cuttage vessel;
5)It is managed after cuttage:Water planting room temperature is 18-28 DEG C, humidity 80-100%, and nutrient solution is replaced in timing, is cultivated 1-4 weeks;
6)Acclimatization and transplants:When the clematis cuttings root long of water planting reaches 1-2cm, which is moved into seedling-growing container, after transplanting Pour 1 water daily, see after 3 days it is dry see pour, avoid direct sunlight, obtain seedling.
2. the method that clematis Water culture according to claim 1 is taken root, it is characterised in that:The plant growth regulating Agent selects the methyl α-naphthyl acetate of a concentration of 200mg/L(NAA)Or the indolebutyric acid of a concentration of 100mg/L(IBA).
3. the method that clematis Water culture according to claim 1 is taken root, it is characterised in that:In the mill water culture nutrient solution Nutrient is with densimeter, N:1.25-2.0 mM/L、P:0.15-0.2 mM/L、K:0.4-0.8 mM/L、Ca:0.4-1.0 mM/L、Mg:0.4-1.0 mM/L、Fe:10-15 mM/L、Mn:3-6 mM/L、Mo:0.1-0.35 mM/L、B:10-18 mM/L、 Zn:0.3-0.7 mM/L、Cu:A concentration of 20-40 mM/L of 0.15-0.3 mM/L, inorganic chelator EDTA-Na2, and pass through It adds in HCl solution and carries out pH value adjusting.
4. the method that clematis Water culture according to claim 1 is taken root, it is characterised in that:The cuttage vessel is width 20-30cm, long 30-40cm, depth 10-15cm plastic box, the cuttage fixed plate for width 20-30cm, long 30-40cm, thickness The PVC foam plate of 0.8-1.5cm, size just cover cuttage vessel, and the cuttage fixed plate is equipped with a diameter of 2.5cm's Circular hole places mouth as cuttings.
5. the method that clematis Water culture according to claim 4 is taken root, it is characterised in that:The cuttage fixed plate is used 30%NaClO carries out disinfection, and is cleaned after impregnating 20min.
6. the method that clematis Water culture according to claim 1 is taken root, it is characterised in that:Every 3 days of mill water culture nutrient solution It replaces once, and mill water culture nutrient solution is kept to be contacted with water-absorbing sponge item, while avoid direct sunlight, keep room ventilation good, Water planting is until take root.
7. the method that clematis Water culture according to claim 1 is taken root, it is characterised in that:The seedling cultivation depth It is advisable with covering new root just, seedling medium is uniformly mixed for peat with perlite, mixed proportion 1:1.
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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN108834860A (en) * 2018-07-09 2018-11-20 中国科学院昆明植物研究所 Rare and endangered medicinal plant swordleaf dragon tree water planting Rapid Rooting seedling establishment method
CN109716940A (en) * 2019-01-25 2019-05-07 福建农林大学 A kind of method of indoor quickly breeding vine tea seedling
CN111133903A (en) * 2020-01-16 2020-05-12 青岛农业大学 Alfalfa water culture cutting propagation method

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CN104082152A (en) * 2014-08-04 2014-10-08 云南农业大学 Tissue culture and rapid propagation method for clematis ranunculoides
CN104396750A (en) * 2014-11-18 2015-03-11 罗翼 Tissue culture propagation method for clematis lanuginosa
CN105145176A (en) * 2015-09-01 2015-12-16 张莘蔓 Method for water culture cutting propagation of callistemon cirtinus
CN106818443A (en) * 2017-03-24 2017-06-13 卢鹏 A kind of red maple bough water planting cuttage breeding method

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834860A (en) * 2018-07-09 2018-11-20 中国科学院昆明植物研究所 Rare and endangered medicinal plant swordleaf dragon tree water planting Rapid Rooting seedling establishment method
CN109716940A (en) * 2019-01-25 2019-05-07 福建农林大学 A kind of method of indoor quickly breeding vine tea seedling
CN111133903A (en) * 2020-01-16 2020-05-12 青岛农业大学 Alfalfa water culture cutting propagation method
CN111133903B (en) * 2020-01-16 2022-01-28 青岛农业大学 Alfalfa water culture cutting propagation method

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