CN106665307A - Efficient sweet osmanthus cutting seedling raising method - Google Patents
Efficient sweet osmanthus cutting seedling raising method Download PDFInfo
- Publication number
- CN106665307A CN106665307A CN201611215977.0A CN201611215977A CN106665307A CN 106665307 A CN106665307 A CN 106665307A CN 201611215977 A CN201611215977 A CN 201611215977A CN 106665307 A CN106665307 A CN 106665307A
- Authority
- CN
- China
- Prior art keywords
- cuttage
- days
- water
- points
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of garden cultivation and particularly relates to an efficient sweet osmanthus cutting seedling raising method. Cuttage is soilless substrate-water culture cuttage, microbial bacterial powder water solutions are used for watering after cuttage, and the microbial bacterial powder water solutions include a bacillus water solution, a lactic acid bacteria water solution and an actinomycetes water solution. The efficient sweet osmanthus cutting seedling raising method comprises the specific steps of culture plate preparation, substrate preparation, substrate laying, cutting processing, cuttage water culture, filing covering, strain conditioning, water and fertilizer management, seedling hardening and transplanting. The efficient sweet osmanthus cutting seedling raising method is high in survival rate, makes root systems vigorous and developed and grow healthily and strongly, and a cultivation period is short.
Description
Technical field
The invention belongs to gardens technical field of cultivation, the efficient cuttage and seedling culture method of more particularly to a kind of Flos Osmanthi Fragrantis.
Background technology
Flos Osmanthi Fragrantis also known as wooden slippers, have the cultivation history of more than 2500 years in China, with abundant germ plasm resource and genetic diversity
Property, it is the distinctive ornamencal flower and tree of China and fragrant seeds.Flos Osmanthi Fragrantis are one of great tradition famous flowers of China ten, and evergreen all the year round, leaf color is dense
Strongly fragrant, the florescence can open for two seasons every year, and fragrance is strong, and delicate fragrance is far overflow, and is widely used in the green of gardens, garden, scenic spot etc.
Change.
At present, the reproduction technique of Flos Osmanthi Fragrantis is mainly sowing, cuttage, grafting etc..Seed propagation is mainly in knot of annual florescence
Beam gathers fragrans seed, and seed propagation is carried out after storage.But most of Flos Osmanthi Fragrantis cultivar factor rooms occur to degenerate and can not tie
It is real, so seminal propagation has certain limitation.Further, since the plant early flowering season that seed sowing is obtained is later, field planting 10
Nian Houcai starts to bloom, and spend it is little, spend few, hereditary character is unstable, is typically only used as being used when stock or breeding.
Flos Osmanthi Fragrantis grafting does sth. in advance 1-2 and blooms than cutting seedling, and what is had even can bloom then, and grafting can keep kind
Good characteristic, and the adaptability of the resistance raising osmanthus fragrans using stock, relatively cold-resistant and Salt And Alkali Tolerance, can expand Flos Osmanthi Fragrantis
Introducing and planting scope.But because graft technology has high demands, there can only be skilled craftsman's manual operations at present, large-scale production is subject to
Certain restriction；It is at present in production to adopt Ligustrum quihoui, water wax, ligustrum lucidum ait etc. to do stock more, but the grafting later stage easily occurs
The uncoordinated phenomenon such as up-thin-low-thick or upper coarse and lower fine, causes aesthetics to reduce.
Flos Osmanthi Fragrantis cuttage can be divided into cuttage and epicormic branch cuttage according to the difference of branch degree of lignification.Using spring tip skewer
Insert with June most preferably, using summer branch cuttage preferably September part, the two seasons are adapted to plant growing and wound healing.Flos Osmanthi Fragrantis skewer
Inserting needs the cuttage environment such as suitable temperature, humidity and illumination condition just to can guarantee that surviving for cutting.Conventional at present has routine
Cuttage, closing cuttage and full exposure spraying three kinds of cuttage forms of cuttage, though and Camless valve train technology has research to fail giving birth to
Widely apply on product, Water culture is taken root difficulty due to xylophyta, bigger using difficulty.Currently for the Camless valve train of Flos Osmanthi Fragrantis
Carry out in substrate more, require in substrate configuration process strictly, and it is relatively costly, heavy industrialization is still not suitable at present should
With.
The content of the invention
To solve above-mentioned technical problem, the present invention is intended to provide a kind of efficient cuttage and seedling culture method of Flos Osmanthi Fragrantis, the method employing
Soilless hydroponic culture promotes Flos Osmanthi Fragrantis to take root, and production process is simple to operate, can carry out indoors, convenient control ambient temperature, cultivation period
It is short.
For achieving the above object, the present invention is achieved through the following technical solutions：
A kind of efficient cuttage and seedling culture method of Flos Osmanthi Fragrantis, the cuttage is soilless substrate-Water culture, and microbial bacteria is used after cuttage
Amidin waters, and it is water-soluble that the microbial bacteria amidin is respectively bacillus cereuss aqueous solution, lactic acid bacteria aqueous solution, actinomycetes
Liquid；Concrete steps prepare including culture plate, matrix treatments, lay substrate, and cutting treatment, cuttage water planting, overlay film, strain is nursed one's health,
Water and fertilizer management, seedling exercising is transplanted；Specifically include following steps：
(1) culture plate prepares：Long 150cm, width 70cm, high 45cm tanks are prepared, support, support are set at the high 20cm of tank
It is upper to place long 30cm, wide 10cm, the seedlings nursing plate of deep 10cm；
(2) matrix treatments：Vermiculitum, perlite, plant ash are exposed to the sun two days, microwave sterilizating is standby；
(3) laying of substrate：Vermiculitum after process is impregnated with nutritional solution, is tiled to bottom seedlings nursing plate, thickness 1-2cm；It is precious
Zhu Yan is thick with the 1-2cm that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg, and the 5cm of plant ash tiling afterwards is standby；
(4) cutting treatment：Strong age elite stand of the life in 10 to 15 years without disease pest, clip tree crown periphery middle and upper part is selected to give birth to wooden then
Change or semi-lignified stalwartness branch, be cut into long 10cm or so, 3 buds of band, top stays 1 pair of leaf, lower end tiltedly to cut away from the lower 0.5cm of section；
Concentration is to soak 1-2h in 30mg/kg ATB rooting agent solutions, dries surface moisture, standby；
(5) cuttage water planting：By in the plant ash in the cutting insertion seedlings nursing plate after process, lower end contacts with Margarita rock stratum,
Irrigated with bacillus cereuss aqueous solution after compacting, bacillus cereuss concentration is 106~108cfu/500ml；
(6) overlay film：Add water in tank so that the water surface is concordant with frame bottom, sink top is covered with transparent plastic film,
Thin film top is covered with 70% commercial shade net；
(7) strain conditioning：Two days after overlay film, film is taken off, divulge information 5h, with lactic acid bacteria aqueous solution plant ash is sprayed, lactic acid bacteria hangs
Liquid concentration is 104~106Cfu/500ml, fountain height is every seedlings nursing plate 250ml；Overlay film after sprinkling, after nursing one's health 2 days, takes off film, divulges information
5h, with actinomycetes aqueous solution plant ash is sprayed, and unwrapping wire bacteria suspension concentration is 106~108Cfu/500ml, fountain height is every seedlings nursing plate
250m1；Overlay film culture is to root eruption after sprinkling；
(8) water and fertilizer management：Surface is sprayed with 1000 times of diluents of EM bacterial manure within 20 days after transplanting, 500g is sprayed per seedlings nursing plate,
Ensure Liquid element arrangements keep nutrition liquid level in tank concordant with support into nutritional solution in tank；
(9) seedling exercising：30 is early 8 points to 9 points daily after inserting, and late 4 points to 5 points, shade net is taken off with thin film, seedling exercising 15 days；
8 points to 10 points of every morning after 45 days, late 3 points to 5 points, shade net is taken off with thin film, seedling exercising 5 days after inserting；Insert latter 50 days often
It early 8 points, at 3 points in afternoon, shade net are taken off with thin film, seedling exercising 10 days；
(10) transplant：60 days seedling exercising are completed after inserting, can be by the Flos Osmanthi Fragrantis seedling replanting for cultivating to big Tanaka, if transplanting time
Temperature is relatively low, notes antifreeze.
Wherein, the support is metal gauze.The face of the tank three is light-proof material, is simultaneously transparent material preparation.
10-15 is inserted during cuttage water planting in each seedlings nursing plate.
As optimal technical scheme, the nutritional solution described in step 3 and step 8 is for composition：Ca(NO3)2·4H2O
2.8mmol/L, KNO34.0mmol/L, MgSO4·7H2O 0.7mmol/L, KH2PO40.9, NH4HPO41.3, NH4NO3
1.7mmol/L, K2HPO40.5mmol/L, NaCl 0.2mmol/L；Disodiumedetate ferrum 75.18umo/L, boric acid
40.30umo/L, manganese sulfate 11.2umo/L, zinc sulfate 1.1umo/L copper sulfate 0.15umo/L ammonium molybdate 0.02umo/L.
As optimal technical scheme, the microwave sterilizating condition in the step (2) is that microwave dose is 3-5kw/kg, microwave
Process time is 5-8min, and microwave treatment temperature is 90 DEG C -100 DEG C.
Soilless hydroponic culture cuttage raise seedling technique is adopted using the present invention, strong root of taking root for 2 months is can reach, basically reaching transplanting will
Ask, with short production cycle, cuttage process labor workload is little, and without the need for site preparation of cutting the earth, cultivating process is simple to operation.
Further to verify effect of the invention, applicant carried out Experimental comparison.
During cuttage of the present invention be compared with remaining several cuttage technique, each cottage method callus is gone out
Between current, the index such as adventitious root time of occurrence, adventitious root bar number, root length, rooting rate is measured.Saw per 3 days in experimentation
Cuttage branch root development situation is examined, each culture plate diverse location takes 10 plants, the plant in 5 culture plates is chosen respectively, altogether
50 plants, take the meansigma methodss of 50 plants of each indexs.Water culture changed a water or nutritional solution per 2 days in continuous mode.Traditional matrices
Cuttage is adopted and identical substrate of the present invention, is Vermiculitum, perlite, plant ash substrate.Traditional earth culture cuttage adopts closed skewer
Slotting method for culturing seedlings is carried out.
First, experimental site overview
Sample plot is located at Hunan Province Yiyang City Yin Feng gardens nursery base, and geographical coordinate is (E110.43, N28.2), and category is sub-
The continental monsoon moist climate in the torrid zone, sunny in base, abundant rainfall has a moderate climate, makes a clear distinction between the four seasons.
2nd, cottage method
2.1 traditional Water cultures：Water planting substrate is done using the tap water for shining one day after, will treat that cuttage branch is in concentration
1-2h is soaked in 30mg/kg ATB rooting agent solutions, surface moisture is dried, in the tap water after insertion solarization, cutting 20 is often processed
Branch, is repeated 3 times.
2.2 suspension culture cuttages：Using shining tap water one day after, by the recipe configuration nutritional solution in the present invention, i.e.,：
Ca(NO3)2·4H2O 2.8mmol/L, KNO34.0mmol/L, MgSO4·7H2O 0.7mmol/L, KH2PO40.9, NH4HPO4
1.3, NH4NO31.7mmol/L, K2HPO40.5mmol/L, NaCl 0.2mmol/L；Disodiumedetate ferrum
75.18umo/L, boric acid 40.30umo/L, manganese sulfate 11.2umo/L, zinc sulfate 1.1umo/L copper sulfate 0.15umo/L ammonium molybdates
0.02umo/L.To treat that cuttage branch soaks 1-2h in concentration is 30mg/kg ATB rooting agent solutions, dry surface moisture, insert
In entering the tap water after shining, cutting 20 is often processed, be repeated 3 times.
2.3 nutritional solution microorganism Water cultures：Nutrient solution is identical with nutritional solution substrate cuttage, operating procedure also phase
Together.It is 10 to add bacillus cereuss to cause bacillus cereuss concentration in nutritional solution after cuttage6~108cfu/500ml；Cultivation is two days later
With lactic acid bacteria aqueous solution is added, lactic acid bacteria suspension concentration is 104~106Cfu/500ml, changes actinomycetes aqueous solution sprinkling base after 2 days
Matter, unwrapping wire bacteria suspension concentration is 106~108cfu/500ml。
2.4 traditional matrices cuttages：Vermiculitum, perlite, plant ash are adopted for substrate, is done by 1: 1: 2.5 mass ratio mixing
Cutting medium.To treat that cuttage branch soaks 1-2h in concentration is 30mg/kg ATB rooting agent solutions, dry surface moisture, insert
Enter cutting, often process cutting 20, be repeated 3 times.
2.5 nutritional solution substrate cuttages：Adopt Vermiculitum, perlite, plant ash for substrate, Vermiculitum is impregnated with nutritional solution, tile
To bottom seedlings nursing plate, thickness 1-2cm；Perlite is thick with the 1-2cm that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg,
The 5cm of plant ash tiling afterwards, it is standby.To treat that cuttage branch soaks 1-2h in concentration is 30mg/kgATB rooting agent solutions, dry in the air
Dry surface moisture, inserts vegetation grieshoch, often processes cutting 20, is repeated 3 times.The nutritional solution and of the present invention group and nutritional solution water
Training group is identical.
2.6 nutritional solution microbial matrices cuttages：Vermiculitum, perlite, plant ash are adopted for substrate, Vermiculitum nutrition immersion
Thoroughly, tile to bottom seedlings nursing plate, thickness 1-2cm；The perlite 1- that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg
2cm is thick, the 5cm of plant ash tiling afterwards, standby.To treat that cuttage branch soaks 1- in concentration is 30mg/kgATB rooting agent solutions
2h, dries surface moisture, inserts vegetation grieshoch, often processes cutting 20, is repeated 3 times.The nutritional solution and of the present invention group and battalion
Nutrient solution water planting group is identical.Irrigated with bacillus cereuss aqueous solution after cuttage, bacillus cereuss concentration is 106~108cfu/500ml；Cultivation
Two days later substrate is sprayed with lactic acid bacteria aqueous solution, lactic acid bacteria suspension concentration is 104~106Cfu/500ml, overlay film after sprinkling, conditioning
After 2 days, film is taken off, divulge information 5h, with actinomycetes aqueous solution substrate is sprayed, unwrapping wire bacteria suspension concentration is 106~108cfu/500ml；Spray
Rear overlay film culture is spilt to root eruption.
2.7 traditional earth culture cuttages：Carry out with reference to the method for Chinese patent CN104663209.
Measurement result is as follows：
The present invention of table 1 and several traditional Flos Osmanthi Fragrantis cuttage technique comparative effectiveness
As shown in Table 1, the sprout time of calluss and indefinite of can significantly shortening compared with traditional cuttage technique of the invention
Root time of occurrence, root hair growth is vigorous in take root quantity and the rooting rate, and rooting process of raising cuttage branch, strong.Experiment
During find, traditional Water culture rooting rate is relatively low, and incision easily occurs to rot, and causes survival rate to reduce, and cuttage process
In need to constantly change water or nutritional solution, cultivating process workload is big, and cost of labor is high.Substrate cuttage root development is slower, growth
Cycle is long, and survival rate is more of the invention to reduce about 30%.Traditional earth culture cutting propagation, nursery early stage site preparation, fertilising are to cultivate successfully
Key, and there is rotten ratio height more of the present invention in cutting seedling bottom, and root growth is slower, and survival rate is reduced about than the present invention
17%.
Rooting rate using nutritional solution microorganism Water culture Flos Osmanthi Fragrantis is minimum, and observation finds that butt rot is serious, it is impossible to raw
Root, root growing way is most weak.Using the more traditional earth culture of nutritional solution substrate microorganism cuttage calluss time of occurrence, water planting and tradition
Substrate cuttage has shifted to an earlier date, but adventitious root time of occurrence has backwardness, and the more traditional earth culture of rooting rate is low, but apparently higher than tradition
Water planting.
In sum, the survival rate and rooting rate of cutting seedling can be effectively improved using the cuttage and seedling culture method of the present invention, is carried
High product quality so that root growth vigor strengthens, and greatly shortens the cutting propagation time.
Description of the drawings
Fig. 1 culture plate structural representations of the present invention.
The implication of each labelling is in figure：1- tanks, 2- supports, 3- vermiculite layers, 4- Margaritas rock stratum, 5- vegetation grieshoch, 6- educates
Seed plate
Specific embodiment
With reference to specific embodiment, the present invention will be further described, it is pointed out that following examples be only with
The form for enumerating is not limited to that to the indicative explaination done of the invention, but protection scope of the present invention.
Embodiment 1
A kind of efficient cuttage and seedling culture method of Flos Osmanthi Fragrantis, cuttage is soilless substrate-Water culture, and microbial germ powder water is used after cuttage
Solution waters, and microbial bacteria amidin is respectively bacillus cereuss aqueous solution, lactic acid bacteria aqueous solution, actinomycetes aqueous solution.Including
Culture plate prepares, matrix treatments, lays substrate, and cutting treatment, cuttage water planting, overlay film, strain conditioning, water and fertilizer management, seedling exercising is moved
Plant；Specifically include following steps：
(1) culture plate prepares：Long 150cm, width 70cm, the tank of high 45cm are prepared, support is set at the high 20cm of tank,
Long 30cm, wide 10cm, the seedlings nursing plate of deep 10cm are placed on frame；As shown in Figure 1；
(2) matrix treatments：Vermiculitum, perlite, plant ash are exposed to the sun two days, microwave sterilizating is standby；
(3) laying of substrate：Vermiculitum after process is impregnated with nutritional solution, is tiled to bottom seedlings nursing plate, thickness 1-2cm；It is precious
Zhu Yan is thick with the 1-2cm that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg, and the 5cm of plant ash tiling afterwards is standby；
(4) cutting treatment：Strong age elite stand of the life in 10 to 15 years without disease pest, clip tree crown periphery middle and upper part is selected to give birth to wooden then
Change or semi-lignified stalwartness branch, be cut into long 10cm or so, 3 buds of band, top stays 1 pair of leaf, lower end tiltedly to cut away from the lower 0.5cm of section；
Concentration is to soak 1-2h in 30mg/kg ATB rooting agent solutions, dries surface moisture, standby；
(5) substrate-Water culture：By in the plant ash in the cutting insertion seedlings nursing plate after process, lower end and Margarita rock stratum
Contact, is irrigated after compacting with bacillus cereuss aqueous solution, and bacillus cereuss concentration is 106~108cfu/500ml；
(6) overlay film：Add water in tank so that the water surface is concordant with frame bottom, sink top is covered with transparent plastic film,
Thin film top is covered with 70% commercial shade net；
(7) strain conditioning：Two days after overlay film, film is taken off, divulge information 5h, with lactic acid bacteria aqueous solution plant ash is sprayed, lactic acid bacteria hangs
Liquid concentration is 104~106Cfu/500ml, fountain height is every seedlings nursing plate 250ml；Overlay film after sprinkling, after nursing one's health 2 days, takes off film, divulges information
5h, with actinomycetes aqueous solution plant ash is sprayed, and unwrapping wire bacteria suspension concentration is 106~108Cfu/500ml, fountain height is every seedlings nursing plate
250ml；Overlay film culture is to root eruption after sprinkling；
(8) water and fertilizer management：Surface is sprayed with 1000 times of diluents of EM bacterial manure within 20 days after transplanting, 500g is sprayed per seedlings nursing plate,
Ensure Liquid element arrangements keep nutrition liquid level in tank concordant with support into nutritional solution in tank；
(9) seedling exercising：30 is early 8 points to 9 points daily after inserting, late 4 points to 5 points, takes shade net and thin film off seedling exercising 15 days；Insert
Afterwards 8 points to 10 points of every morning after 45 days, take shade net and thin film off seedling exercising 5 days at late 3 points to 5 points；Insert latter 50 days early daily
8 points, at 3 points in afternoon, are taken shade net and thin film off seedling exercising 10 days；
(10) transplant：60 days seedling exercising are completed after inserting, can be by the Flos Osmanthi Fragrantis seedling replanting for cultivating to big Tanaka, if transplanting time
Temperature is relatively low, notes antifreeze.
Calluss time of occurrence, adventitious root time of occurrence, adventitious root bar number, adventitious root are long after observation the present embodiment cuttage
And rooting rate index, with clear water, vermiculite matrix culture, traditional earth culture is to do controlled trial, and testing result is as shown in table 2.
Rooting inhibitors after the present embodiment cuttage of table 2
As shown in Table 2, Flos Osmanthi Fragrantis branch calluss time of occurrence, adventitious root time of occurrence have bright after the present embodiment cuttage
Aobvious shifts to an earlier date, and the more traditional earth culture method of adventitious root quantity and root length is more excellent after taking root, and rooting rate is up to more than 90%.
Embodiment 2
A kind of efficient cuttage and seedling culture method of Flos Osmanthi Fragrantis, cuttage is soilless substrate-Water culture, and microbial germ powder water is used after cuttage
Solution waters.Microbial bacteria amidin is respectively bacillus cereuss aqueous solution, lactic acid bacteria aqueous solution, actinomycetes aqueous solution；Specifically
Step prepares including culture plate, matrix treatments, the laying of substrate, cutting treatment, substrate-Water culture, overlay film, strain conditioning,
Water and fertilizer management, seedling exercising is transplanted；Specifically include following steps：
(1) culture plate prepares：Long 150cm, width 70cm, high 45cm tanks are prepared, support, support are set at the high 20cm of tank
It is upper to place long 30cm, wide 10cm, the seedlings nursing plate of deep 10cm；As described in Figure 1, support adopts wire netting, for supporting educating for top
Seed plate.
(2) matrix treatments：Vermiculitum, perlite, plant ash are exposed to the sun two days, microwave sterilizating is standby；The microwave sterilizating bar
It is 3-5kw/kg that part is microwave dose, and microwave treatment time is 5-8min, and microwave treatment temperature is 90 DEG C -100 DEG C；
(3) laying of substrate：Vermiculitum after process is impregnated with nutritional solution, is tiled to bottom seedlings nursing plate, thickness 1-2cm；It is precious
Zhu Yan is thick with the 1-2cm that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg, and the 5cm of plant ash tiling afterwards is standby；
(4) cutting treatment：Strong age elite stand of the life in 10 to 15 years without disease pest, clip tree crown periphery middle and upper part is selected to give birth to wooden then
Change or semi-lignified stalwartness branch, be cut into long 10cm or so, 3 buds of band, top stays 1 pair of leaf, lower end tiltedly to cut away from the lower 0.5cm of section；
Concentration is to soak 1-2h in 30mg/kg ATB rooting agent solutions, dries surface moisture, standby；
(5) substrate-Water culture：By in the plant ash in the cutting insertion seedlings nursing plate after process, lower end and Margarita rock stratum
Contact, is irrigated after compacting with bacillus cereuss aqueous solution, and bacillus cereuss concentration is 106~108cfu/500ml；
(6) overlay film：Add water in tank so that the water surface is concordant with frame bottom, sink top is covered with transparent plastic film,
Thin film top is covered with 70% commercial shade net；
(7) strain conditioning：Two days after overlay film, film is taken off, divulge information 5h, with lactic acid bacteria aqueous solution plant ash is sprayed, lactic acid bacteria hangs
Liquid concentration is 104~106Cfu/500ml, fountain height is every seedlings nursing plate 250ml；Overlay film after sprinkling, after nursing one's health 2 days, takes off film, divulges information
5h, with actinomycetes aqueous solution plant ash is sprayed, and unwrapping wire bacteria suspension concentration is 106~108Cfu/500ml, fountain height is every seedlings nursing plate
250ml；Overlay film culture is to root eruption after sprinkling；
(8) water and fertilizer management：Surface is sprayed with 1000 times of diluents of EM bacterial manure within 20 days after transplanting, 500g is sprayed per seedlings nursing plate,
Ensure Liquid element arrangements keep nutrition liquid level in tank concordant with support into nutritional solution in tank；
(9) seedling exercising：30 is early 8 points to 9 points daily after inserting, late 4 points to 5 points, takes shade net and thin film off seedling exercising 15 days；Insert
Afterwards 8 points to 10 points of every morning after 45 days, take shade net and thin film off seedling exercising 5 days at late 3 points to 5 points；Insert latter 50 days early daily
8 points, at 3 points in afternoon, are taken shade net and thin film off seedling exercising 10 days；
(10) transplant：60 days seedling exercising are completed after inserting, can be by the Flos Osmanthi Fragrantis seedling replanting for cultivating to big Tanaka, if transplanting time
Temperature is relatively low, notes antifreeze.
Calluss time of occurrence, adventitious root time of occurrence, adventitious root bar number, adventitious root are long after observation the present embodiment cuttage
And rooting rate index, with clear water, vermiculite matrix culture, Vermiculitum sterilization matrix culture group, traditional earth culture as controlled trial is done, detect
As a result it is as shown in table 3.
Rooting inhibitors after the present embodiment cuttage of table 3
As shown in Table 2, Flos Osmanthi Fragrantis branch calluss time of occurrence, adventitious root time of occurrence have bright after the present embodiment cuttage
Aobvious shifts to an earlier date, and the more traditional earth culture method of adventitious root quantity and root length is more excellent after taking root, and rooting rate is up to more than 90%.Identical
Sterilising conditions under, cultivated with the Vermiculitum after sterilizing, although significantly improve the rooting rate of Flos Osmanthi Fragrantis Seedling, but its adventitious root goes out
Postpone between current, and adventitious root bar number is less, adventitious root length is shorter.Find during cuttage, though after Vermiculitum is sterilized
So cuttage branch rotting rate reduction, but the growth promoter of its root becomes excellent expected from not occurring, and this is likely due to Vermiculitum
Beneficial microbe therein is killed in the lump after sterilizing, causes to promote the probioticss quantity of plant root development to reduce, so as to lead
Root development is caused to slow down.
Embodiment 3
A kind of efficient cuttage and seedling culture method of Flos Osmanthi Fragrantis, cuttage is soilless hydroponic culture cuttage, and microbial bacteria amidin is used after cuttage
Water.Microbial bacteria amidin is respectively bacillus cereuss aqueous solution, lactic acid bacteria aqueous solution, actinomycetes aqueous solution；Step includes
Culture plate prepares, matrix treatments, the laying of substrate, cutting treatment, substrate-Water culture, overlay film, strain conditioning, water and fertilizer management,
Seedling exercising, transplants；Specifically include following steps：
(1) culture plate prepares：Long 150cm, width 70cm, high 45cm tanks are prepared, support, support are set at the high 20cm of tank
It is upper to place long 30cm, wide 10cm, the seedlings nursing plate of deep 10cm；As described in Figure 1, support adopts wire netting, for supporting educating for top
Seed plate；The face of tank three is light-proof material, is simultaneously transparent material preparation, convenient selectively to absorb sunlight photograph by cutting seedling
Penetrate；
(2) matrix treatments：Vermiculitum, perlite, plant ash are exposed to the sun two days, microwave sterilizating is standby；The microwave sterilizating bar
It is 3-5kw/kg that part is microwave dose, and microwave treatment time is 5-8min, and microwave treatment temperature is 90 DEG C -100 DEG C；
(3) laying of substrate：Vermiculitum after process is impregnated with nutritional solution, is tiled to bottom seedlings nursing plate, thickness 1-2cm；It is precious
Zhu Yan is thick with the 1-2cm that tiles after the ATB rooting agent solutions immersion of 50~100mg/kg, and the 5cm of plant ash tiling afterwards is standby；Battalion
Nutrient solution formula is：Ca(NO3)2·4H2O 2.8mmol/L, KNO34.0mmol/L, MgSO4·7H2O 0.7mmol/L, KH2PO4
0.9, NH4HPO41.3, NH4NO31.7mmol/L, K2HPO40.5mmol/L, NaCl 0.2mmol/L；Ethylenediaminetetraacetic acid two
Sodium ferrum 75.18umo/L, boric acid 40.30umo/L, manganese sulfate 11.2umo/L, zinc sulfate 1.1umo/L copper sulfate 0.15umo/L molybdenums
Sour ammonium 0.02umo/L；
(4) cutting treatment：Strong age elite stand of the life in 10 to 15 years without disease pest, clip tree crown periphery middle and upper part is selected to give birth to wooden then
Change or semi-lignified stalwartness branch, be cut into long 10cm or so, 3 buds of band, top stays 1 pair of leaf, lower end tiltedly to cut away from the lower 0.5cm of section；
Concentration is to soak 1-2h in 30mg/kg ATB rooting agent solutions, dries surface moisture, standby；
(5) substrate-Water culture：By in the plant ash in the cutting insertion seedlings nursing plate after process, insert in each seedlings nursing plate
10-15, cutting lower end contacts with Margarita rock stratum, is irrigated with bacillus cereuss aqueous solution after compacting, and bacillus cereuss concentration is 106~
108cfu/500ml；
(6) overlay film：Add water in tank so that the water surface is concordant with frame bottom, sink top is covered with transparent plastic film,
Thin film top is covered with 70% commercial shade net；
(7) strain conditioning：Two days after overlay film, film is taken off, divulge information 5h, with lactic acid bacteria aqueous solution plant ash is sprayed, lactic acid bacteria hangs
Liquid concentration is 104~106Cfu/500ml, fountain height is every seedlings nursing plate 250ml；Overlay film after sprinkling, after nursing one's health 2 days, takes off film, divulges information
5h, with actinomycetes aqueous solution plant ash is sprayed, and unwrapping wire bacteria suspension concentration is 106~108Cfu/500ml, fountain height is every seedlings nursing plate
250ml；Overlay film culture is to root eruption after sprinkling；
(8) water and fertilizer management：Surface is sprayed with 1000 times of diluents of EM bacterial manure within 20 days after transplanting, 500g is sprayed per seedlings nursing plate,
Ensure Liquid element arrangements keep nutrition liquid level in tank concordant with support into nutritional solution in tank；
(9) seedling exercising：30 is early 8 points to 9 points daily after inserting, late 4 points to 5 points, takes shade net and thin film off seedling exercising 15 days；Insert
Afterwards 8 points to 10 points of every morning after 45 days, take shade net and thin film off seedling exercising 5 days at late 3 points to 5 points；Insert latter 50 days early daily
8 points, at 3 points in afternoon, are taken shade net and thin film off seedling exercising 10 days；
(10) transplant：60 days seedling exercising are completed after inserting, can be by the Flos Osmanthi Fragrantis seedling replanting for cultivating to big Tanaka, if transplanting time
Temperature is relatively low, notes antifreeze.
Calluss time of occurrence, adventitious root time of occurrence, adventitious root bar number, adventitious root are long after observation the present embodiment cuttage
And rooting rate index, it is micro- with clear water, Vermiculitum group, Vermiculitum sterilizing group, Vermiculitum nutritional solution group, Vermiculitum microorganism group, Vermiculitum nutritional solution
Biological group, earth culture group compare, testing result is as shown in table 4.
Rooting inhibitors after the present embodiment cuttage of table 4
In table 4, each treatment conditions are as follows：
Clear water group：With clear water as culture matrix, a water is changed within two days；
Vermiculitum group：With Vermiculitum as culture medium, water as needed, carry out by traditional matrices cottage method；
Vermiculitum sterilizing group：Vermiculitum is carried out using the present embodiment identical method to do culture matrix after microwave sterilizating, other
Cultural method is identical with Vermiculitum group；
Vermiculitum nutritional solution group：With Vermiculitum as culture medium, poured using the present embodiment identical nutritional solution in cultivation
Water, is needed to water by seedling growth；
Vermiculitum microorganism group：With Vermiculitum as culture medium, entered using the present embodiment identical microbial inoculum in cultivation
Row pouring, irrigation amount and time same the present embodiment；
Vermiculitum nutritional solution microorganism group：With Vermiculitum as culture medium, the present embodiment identical microorganism is adopted in cultivation
Bacterium solution is poured, irrigation amount and time same the present embodiment.Meanwhile, dose adopts the nutrition of the present embodiment during cuttage
Liquid；
Earth culture group：Carry out according to traditional closed earth culture cottage method.
As shown in Table 4, calluss time of occurrence after cuttage, adventitious root time of occurrence, adventitious root bar number, adventitious root length and
The indexs such as rooting rate are superior to other experimental grouies.Add nutritional solution, microorganism etc. on the basis of traditional matrices culture can not
Reach preferable effect.The characteristics of method of the present embodiment has prominent and progress.
Claims (6)
1. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis, it is characterised in that the cuttage is soilless substrate-Water culture, after cuttage
Watered with microbial bacteria amidin, the microbial bacteria amidin be respectively bacillus cereuss aqueous solution, lactic acid bacteria aqueous solution,
Actinomycetes aqueous solution；Concrete steps prepare including culture plate, matrix treatments, the laying of substrate, cutting treatment, substrate-water planting skewer
Insert, overlay film, strain conditioning, water and fertilizer management, seedling exercising is transplanted；Specifically include following steps：
(1) culture plate prepares：Long 150cm, width 70cm, high 45cm tanks are prepared, support is set at the high 20cm of tank, put on support
Put long 30cm, wide 10cm, the seedlings nursing plate of deep 10cm；
(2) matrix treatments：Vermiculitum, perlite, plant ash are exposed to the sun two days, microwave sterilizating is standby；
(3) laying of substrate：Vermiculitum after process is impregnated with nutritional solution, is tiled to bottom seedlings nursing plate, thickness 1-2cm；Perlite
With tiling after the ATB rooting agent solutions immersion of 50~100mg/kg, 1-2cm is thick, and the 5cm of plant ash tiling afterwards is standby；
(4) cutting treatment：Select strong age elite stand of the life in 10 to 15 years without disease pest, clip tree crown periphery middle and upper part give birth to then lignifying or
Semi-lignified stalwartness branch, is cut into long 10cm or so, 3 buds of band, and top stays 1 pair of leaf, lower end tiltedly to cut away from the lower 0.5cm of section；In concentration
To soak 1-2h in 30mg/kg ATB rooting agent solutions, surface moisture is dried, it is standby；
(5) cuttage water planting：By in the plant ash in the cutting insertion seedlings nursing plate after process, lower end contacts with Margarita rock stratum, compacting
Irrigated with bacillus cereuss aqueous solution afterwards, bacillus cereuss concentration is 106~108cfu/500ml；
(6) overlay film：Add water in tank so that the water surface is concordant with frame bottom, sink top is covered with transparent plastic film, thin film
Top is covered with 70% commercial shade net；
(7) strain conditioning：Two days after overlay film, film is taken off, divulge information 5h, and with lactic acid bacteria aqueous solution plant ash is sprayed, and lactic acid bacteria suspension is dense
Spend for 104~106Cfu/500ml, fountain height is every seedlings nursing plate 250ml；Overlay film after sprinkling, after nursing one's health 2 days, takes off film, and divulge information 5h,
Plant ash is sprayed with actinomycetes aqueous solution, unwrapping wire bacteria suspension concentration is 106~108Cfu/500ml, fountain height is every seedlings nursing plate
250ml；Overlay film culture is to root eruption after sprinkling；
(8) water and fertilizer management：Surface is sprayed with 1000 times of diluents of EM bacterial manure within 20 days after transplanting, 500g, Xiang Shui are sprayed per seedlings nursing plate
Ensure Liquid element arrangements keep nutrition liquid level in tank concordant with support into nutritional solution in groove：
(9) seedling exercising：30 is early 8 points to 9 points daily after inserting, and late 4 points to 5 points, shade net is taken off with thin film, seedling exercising 15 days；After inserting
8 points to 10 points of every morning after 45 days, shade net are taken off with thin film by late 3 points to 5 points, seedling exercising 5 days；50 days daily early 8 after inserting
Point is taken off shade net and thin film, seedling exercising 10 days at 3 points in afternoon；
(10) transplant：60 days seedling exercising are completed after inserting, can be by the Flos Osmanthi Fragrantis seedling replanting for cultivating to big Tanaka, if transplanting time temperature
It is relatively low, note antifreeze.
2. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis as claimed in claim 1, it is characterised in that：The support is tinsel
Net.
3. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis as claimed in claim 1, it is characterised in that：The face of the tank three is impermeable
Luminescent material, is simultaneously transparent material preparation.
4. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis as claimed in claim 1, it is characterised in that：During cuttage water planting each
10-15 is inserted in seedlings nursing plate.
5. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis as claimed in claim 1, it is characterised in that：Described in step 3 and step 8
Nutritional solution be for composition：Ca(NO3)2·4H2O 2.8mmol/L, KNO34.0mmol/L, MgSO4·7H2O 0.7mmol/L,
KH2PO40.9, NH4HPO41.3, NH4NO31.7mmol/L, K2HPO40.5mmol/L, NaCl 0.2mmol/L；Ethylenediaminetetraacetic acid
Disodium iron 75.18umo/L, boric acid 40.30umo/L, manganese sulfate 11.2umo/L, zinc sulfate 1.1umo/L copper sulfate 0.15umo/L
Ammonium molybdate 0.02umo/L.
6. the efficient cuttage and seedling culture method of a kind of Flos Osmanthi Fragrantis as claimed in claim 1, it is characterised in that：It is micro- in the step (2)
It is 3-5kw/kg that ripple sterilising conditions are microwave dose, and microwave treatment time is 5-8min, and microwave treatment temperature is 90 DEG C -100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611215977.0A CN106665307A (en) | 2016-12-26 | 2016-12-26 | Efficient sweet osmanthus cutting seedling raising method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611215977.0A CN106665307A (en) | 2016-12-26 | 2016-12-26 | Efficient sweet osmanthus cutting seedling raising method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106665307A true CN106665307A (en) | 2017-05-17 |
Family
ID=58871419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611215977.0A Pending CN106665307A (en) | 2016-12-26 | 2016-12-26 | Efficient sweet osmanthus cutting seedling raising method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106665307A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155782A (en) * | 2017-06-22 | 2017-09-15 | 和县赭洛山茶叶种植专业合作社 | A kind of sweet osmanthus cuttage and seedling culture method |
| CN113261438A (en) * | 2021-06-08 | 2021-08-17 | 中国医学科学院药用植物研究所 | Method for promoting transplanting survival of pilose antler grass stem cutting seedlings |
| CN116569843A (en) * | 2023-06-14 | 2023-08-11 | 中国林业科学研究院林业研究所 | Method for shallow liquid rooting of idesia polycarpa |
-
2016
- 2016-12-26 CN CN201611215977.0A patent/CN106665307A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155782A (en) * | 2017-06-22 | 2017-09-15 | 和县赭洛山茶叶种植专业合作社 | A kind of sweet osmanthus cuttage and seedling culture method |
| CN113261438A (en) * | 2021-06-08 | 2021-08-17 | 中国医学科学院药用植物研究所 | Method for promoting transplanting survival of pilose antler grass stem cutting seedlings |
| CN116569843A (en) * | 2023-06-14 | 2023-08-11 | 中国林业科学研究院林业研究所 | Method for shallow liquid rooting of idesia polycarpa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104303969A (en) | Chinese rose water planting cutting propagation method | |
| CN100426952C (en) | Tissue culture seedling-growing method for bird's-net fern | |
| CN103931473B (en) | A kind of method spending the efficient Fast-propagation of tomonea in vain | |
| CN103404349B (en) | Cultivation method for dwarf potted rixianggui (variety of sweet-scented osmanthus) | |
| CN104904502A (en) | Method for grating potted seedling cultivation of watermelon in summer | |
| CN107018896B (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN103843540A (en) | Photinia serrulata plantlet cutting propagation method | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN107182754B (en) | Container soilless culture process for peony | |
| CN104542281A (en) | Tissue culture and propagation method of viburnum tinus | |
| CN106665307A (en) | Efficient sweet osmanthus cutting seedling raising method | |
| CN102349430A (en) | Method for breeding seedlings of grapes by using containers | |
| CN109328959A (en) | A kind of cultural method of cuckoo new varieties | |
| CN107660464B (en) | Tissue culture rapid propagation method for excellent seedlings of sequoia sempervirens | |
| CN102057871B (en) | Method for breeding cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for north latitude 26 to 30 degrees | |
| CN103461029A (en) | Flower-fruit pot soil structure and preparation method thereof | |
| CN108124749A (en) | A kind of method that clematis Water culture is taken root | |
| CN107211717A (en) | A kind of muskmelon heliogreenhouse early spring, strawberry cultivation method autumn and winter | |
| CN106171713A (en) | A kind of cultural method of southern magnolia | |
| CN106105641A (en) | A kind of cultural method of southern magnolia | |
| CN107683768B (en) | A kind of acclimatization and transplants method of the syringa reticulata var mandshurica tissue-cultured seedling of callus induction | |
| CN110122333A (en) | A kind of method that flame tree seed stratification is taken root | |
| CN104982188A (en) | Planting method for petunia hybrida | |
| CN103609415B (en) | A kind of matrix of cultivating climbing plant and the method using matrix cottage propagation | |
| CN105961126A (en) | Southern magnolia cultivation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170517 |
|
| WD01 | Invention patent application deemed withdrawn after publication |