CN103636373B - One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant - Google Patents

One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant Download PDF

Info

Publication number
CN103636373B
CN103636373B CN201310536612.8A CN201310536612A CN103636373B CN 103636373 B CN103636373 B CN 103636373B CN 201310536612 A CN201310536612 A CN 201310536612A CN 103636373 B CN103636373 B CN 103636373B
Authority
CN
China
Prior art keywords
fresh
bog moss
alive
plant
cuttings
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201310536612.8A
Other languages
Chinese (zh)
Other versions
CN103636373A (en
Inventor
陈英敏
郑云普
王贺新
乌凤章
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dalian University
Original Assignee
Dalian University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dalian University filed Critical Dalian University
Priority to CN201310536612.8A priority Critical patent/CN103636373B/en
Publication of CN103636373A publication Critical patent/CN103636373A/en
Application granted granted Critical
Publication of CN103636373B publication Critical patent/CN103636373B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention belongs to technical field of plant asexual propagation, be specifically related to one and utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, be particularly useful for carrying out fast breeding to some main genus vaccinium plants.Choose blueberry raw semi-lignified branch or tissue culturing seedling's stem section then, with cuttings base portion after soaking with indole butyric acid 2 hours.The endogenous growth model extracted from genus vaccinium plant is inoculated in fresh and alive bog moss culture matrix.Cutting is inserted fresh and alive bog moss matrix, then carry out plastic mulching.After cuttage, temperature controls at 15 ~ 28 DEG C, and relative moisture controls 80 ~ 90%, after skewer rooting of cuttings, at interval of within 20 days, applying the liquid complete value fertilizer that concentration is about 0.5%.This method utilizes fresh and alive bog moss as the matrix of genus vaccinium plant cuttage nursery, and improves the survival rate of seedling of genus vaccinium plant by the mycorhiza of inoculation genus vaccinium plant, ensures to cultivate stalwartness, high quality seedling without damage by disease and insect.

Description

One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant
Technical field
The invention belongs to technical field of plant asexual propagation, be specifically related to one and utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, be particularly useful for carrying out fast breeding to some main genus vaccinium plants.
Technical background
Genus vaccinium plant is under the jurisdiction of Ericaceae, is generally short and small shrub.The pulp of this genus most plants fruit is fine and smooth, and pericarp is thin, and seed is minimum, and juice is many, sweet and sour palatability, both can eat raw, also can be processed into jam, preserved fruit, fruit drink etc.Its fruit, except containing except conventional sugar, organic acid and vitamin C, is also rich in the trace element such as rare special composition and abundant potassium, iron, zinc, manganese in other fruit such as anthocyan, vitamin E, vitamin A, Cobastab, SOD, protein, fat.At pre-myopia prevention, strengthen in cardiac function, anticancer, anti-cardiovascular disease there is extremely important effect.
Cuttage is as the important means of seedling-wood breeding, simple and easy to do, and can breed out a large amount of breeding nursery stocks within a short period of time, is thus widely used.One of successful key factor of blueberry cuttage and seedling culture is the selection of matrix.The effect of matrix, except support and fixing plant, the more important thing is that as moisture, air, nutrient, acid-base value etc., and these are all relevant with the physicochemical character of matrix for plant growth provides a root system environment stablized, be suitable for.The particle size, density, total porosity etc. of its mesostroma are important character parameters.At present, the matrix of genus vaccinium plant cuttage mainly contains the types such as vermiculite, the peat composed of rotten mosses, river sand.But result of study in the past shows, the success rate utilizing above-mentioned several matrix to carry out cuttage to genus vaccinium plant is lower, is not ideal cutting medium.Research in recent years finds that bryophyte has the features such as water retention property is strong, air capacity of soils good, corrosion-resistant, temperature changing amplitude is little, bog moss is as the moss of extensively distribution in global range, especially extremely common in the Changbaishan area in China northeast, and find that genus vaccinium plant likes the growth of same bog moss together usually through our previous research, there is a kind of relation of symbiosis between them, in bog moss plant corpus, there is the endotrophic mycorrhiza identical with genus vaccinium plant.
Comprise bog moss based on this research and utilization bryophyte also had, as matrix, cottage propagation is carried out to genus vaccinium plant, but, and the liver moss of not all can as the desirable matrix of genus vaccinium plant cuttage, the non-fresh and alive property bryophyte of most employing is as matrix, in medium, add various bactericide simultaneously, thus making nursery reproductive survival rate low, cutting experiment is undesirable.Therefore, fresh and alive bog moss is utilized to be carry out vegetative a kind of effective method to genus vaccinium plant as genus vaccinium plant cuttage matrix.
Summary of the invention
For the present situation of current genus vaccinium plant asexual multiplication technology, the present invention is on the basis of understanding genus vaccinium plant seedling growth characteristic in depth, one is provided to utilize fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, utilize fresh and alive bog moss as the matrix of genus vaccinium plant cuttage nursery, and the survival rate of seedling of genus vaccinium plant is improved by the mycorhiza of inoculation genus vaccinium plant, ensure to cultivate stalwartness, high quality seedling without damage by disease and insect.
To achieve these goals, the present invention realizes as follows: one utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant, it is characterized in that:
(1) selection of cuttings and process: choosing then raw semi-lignified, fringe bar sturdy logical straight, leaf bud is full, blade does cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to calli induction, and then formation young root, long 6 ~ the 8cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: be first inoculated into being mixed with mycorhiza suspension by the VA Mycorrhizal Fungi spore extracted in great bilberry in a small amount of fresh and alive bog moss plant cultivated in Experimental Establishment, make the propagation that endotrophic mycorrhiza is a large amount of wherein; Then, the above-mentioned liver moss inoculated is utilized to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) ensures that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) ensure that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix used within 30 days.
(3) seedbed prepares: cuttage the last week, and above-mentioned bog moss matrix is laid into seedbed, can place one deck Polypropylence Sheet, non-woven fabrics or be other planting soils and bare area below slotting machine.
(4) cuttage: by the fresh and alive bog moss matrix of middle for step (1) the cuttings inserting step (2) processed, water content of substrate remains on 50 ~ 70%, porosity 60 ~ 70%, insertion depth 1 ~ 3cm, be uncovered with bud and blade and be advisable, carry out plastic mulching and shade.
(5) manage after cuttage: every day spray cooling, temperature control 15 ~ 28 DEG C, humid control is about 80 ~ 90%.After cuttage rooting at noon 12 o'clock to 15 time remove plastic film, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Applying N: P: K be 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, sprays 1 time at interval of 20d.
The described fresh and alive bog moss that utilizes carries out vegetative method to genus vaccinium plant, in order to ensure the activity extracting mycorhiza from genus vaccinium plant, does not add any bactericide in culture matrix.Adopt this method, cuttings starts to take root after cuttage 20d, and after 30 ~ 40d, survival rate is more than 90%, and seedling growth is healthy and strong, breeds efficiency and significantly improves.
Of the present inventionly effectively to be: compared with genus vaccinium plant asexual multiplication technology in the past, this method utilizes fresh and alive bog moss as the matrix of genus vaccinium plant cuttage nursery, and the survival rate of seedling of genus vaccinium plant is improved by the mycorhiza of inoculation genus vaccinium plant, ensure to cultivate stalwartness, high quality seedling without damage by disease and insect.
Embodiment
Different substrates water content and the cuttage degree of depth is described in the present invention in detail on the impact (for blueberry, in table 1 and table 2) of genus vaccinium plant cuttage survival rate below in conjunction with specific embodiment.
Embodiment one
(1) selection of cuttings and process: choosing then raw semi-lignified, fringe bar sturdy logical straight, leaf bud is full, blade does cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to calli induction, and then formation young root, the long 6cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: be first inoculated into being mixed with mycorhiza suspension by the VA Mycorrhizal Fungi spore extracted in great bilberry in a small amount of fresh and alive bog moss plant cultivated in Experimental Establishment, make the propagation that endotrophic mycorrhiza is a large amount of wherein; Then, the above-mentioned liver moss inoculated is utilized to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) ensures that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) ensure that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix used within 30 days.
(3) seedbed prepares: cuttage the last week, and above-mentioned bog moss matrix is laid into seedbed, can place one deck Polypropylence Sheet, non-woven fabrics or be other planting soils and bare area below slotting machine.
(4) cuttage: by the fresh and alive bog moss matrix of middle for step (1) the cuttings inserting step (2) processed, water content of substrate remains on 50%, porosity 60%, insertion depth 1cm, is uncovered and is advisable, carry out plastic mulching and shade with bud and blade.
(5) manage after cuttage: every day spray cooling, temperature controls 15 DEG C, and humid control is about 80%.After cuttage rooting at noon 12 o'clock to 15 time remove plastic film, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Applying N: P: K be 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, sprays 1 time at interval of 20d.
Embodiment two
(1) selection of cuttings and process: choosing then raw semi-lignified, fringe bar sturdy logical straight, leaf bud is full, blade does cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to calli induction, and then formation young root, the long 8cm of cuttings, soaks cuttings base portion 2h with the quickly dipped cutting of indolebutyric acid or with methyl α-naphthyl acetate.
(2) preparation of matrix: be first inoculated into being mixed with mycorhiza suspension by the VA Mycorrhizal Fungi spore extracted in great bilberry in a small amount of fresh and alive bog moss plant cultivated in Experimental Establishment, make the propagation that endotrophic mycorrhiza is a large amount of wherein; Then, the above-mentioned liver moss inoculated is utilized to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza.Described fresh and alive bog moss matrix, must meet following condition: (1) ensures that the liver moss of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) ensure that liver moss water content is more than 50%; (4) fresh and alive liver moss matrix used within 30 days.
(3) seedbed prepares: cuttage the last week, and above-mentioned bog moss matrix is laid into seedbed, can place one deck Polypropylence Sheet, non-woven fabrics or be other planting soils and bare area below slotting machine.
(4) cuttage: by the fresh and alive bog moss matrix of middle for step (1) the cuttings inserting step (2) processed, water content of substrate remains on 70%, porosity 70%, insertion depth 3cm, is uncovered and is advisable, carry out plastic mulching and shade with bud and blade.
(5) manage after cuttage: every day spray cooling, temperature controls 28 DEG C, and humid control is about 90%.After cuttage rooting at noon 12 o'clock to 15 time remove plastic film, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Applying N: P: K be 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, sprays 1 time at interval of 20d.
table 1. different substrates water content is on the impact of genus vaccinium plant cuttage survival rate.
the different cuttage degree of depth of table 2. is on the impact of genus vaccinium plant cuttage survival rate.

Claims (1)

1. utilize fresh and alive bog moss to carry out a vegetative method to genus vaccinium plant, it is characterized in that:
(1) selection of cuttings and process: choosing then raw semi-lignified, fringe bar sturdy logical straight, leaf bud is full, blade does cuttings without the branch of damage by disease and insect, described fringe bar degree of lignification is low, be beneficial to calli induction, and then formation young root, long 6 ~ the 8cm of cuttings, soaks cuttings base portion 2h by the quickly dipped cuttings of indolebutyric acid or with methyl α-naphthyl acetate;
(2) preparation of matrix: be first inoculated into being mixed with mycorhiza suspension by the VA Mycorrhizal Fungi spore extracted in great bilberry in a small amount of fresh and alive bog moss plant cultivated in Experimental Establishment, make the propagation that endotrophic mycorrhiza is a large amount of in fresh and alive bog moss plant; Then, the fresh and alive bog moss plant that inoculated is utilized to be inoculated in fresh and alive bog moss culture matrix as the maternal plant of endotrophic mycorrhiza;
(3) seedbed prepares: cuttage the last week, and above-mentioned fresh and alive bog moss culture matrix is laid into seedbed, be bare area or placement one deck Polypropylence Sheet or layer of non-woven fabric or other planting soils of one deck below slotting machine;
(4) cuttage: by the fresh and alive bog moss culture matrix of middle for step (1) the cuttings inserting step (2) processed, water content of substrate remains on 50 ~ 70%, porosity 60 ~ 70%, insertion depth 1 ~ 3cm, bud and blade are uncovered, and carry out plastic mulching and shade;
(5) manage after cuttage: every day spray cooling, temperature control 15 ~ 28 DEG C, humid control is 80 ~ 90%; After cuttage rooting at noon 12 o'clock to 15 time remove plastic film, reduce humidity, increase illumination, and carry out the work of weeding, insect protected; Applying N: P: K be 13: 26: 13 or N: P: K is the complete value fertilizer of 15: 30: 4, and fertilizer applies with liquid state, sprays 1 time at interval of 20d;
Described fresh and alive bog moss culture matrix, must meet following condition: (1) ensures that the fresh and alive bog moss plant of field acquisition is green fresh and alive state; (2) in transportation, utilize the woven bag of good air permeability; (3) ensure that fresh and alive bog moss plant water content is more than 50%; (4) fresh and alive bog moss culture matrix used within 30 days.
CN201310536612.8A 2013-11-04 2013-11-04 One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant Active CN103636373B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310536612.8A CN103636373B (en) 2013-11-04 2013-11-04 One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310536612.8A CN103636373B (en) 2013-11-04 2013-11-04 One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant

Publications (2)

Publication Number Publication Date
CN103636373A CN103636373A (en) 2014-03-19
CN103636373B true CN103636373B (en) 2016-03-02

Family

ID=50241791

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310536612.8A Active CN103636373B (en) 2013-11-04 2013-11-04 One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant

Country Status (1)

Country Link
CN (1) CN103636373B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104542210B (en) * 2014-11-28 2017-01-25 大连大学 Rapid propagation method for cranberry plant
CN104541887B (en) * 2015-01-09 2017-03-22 云南云秀花卉有限公司 Film-covered Chinese rose single bud cutting propagation method
CN104871762B (en) * 2015-04-29 2017-03-29 中南林业科技大学 Rhododendron auriculatum cuttage breeding method
CN106358890A (en) * 2015-07-23 2017-02-01 程向东 Vaccinium uliginosum cultivation technique
CN106171473A (en) * 2016-08-04 2016-12-07 余庆县农家人绿色食品开发有限公司 The implantation methods of Fructus Capsici
CN109661960B (en) * 2018-08-24 2021-06-15 伊春林业科学院 Wild Vaccinium uliginosum twig micro-cutting seedling raising technology

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101129126B (en) * 2007-10-17 2010-06-02 南京大学 Cottage root promoting method of blueberry green branch
CN101690452B (en) * 2009-09-23 2011-02-09 金陵科技学院 Hardwood cutting and seedling raising method for blueberry
CN102524061B (en) * 2011-12-03 2013-09-04 韦庆和 Rapid breeding technology for blueberry tissue culture seedling micro cuttings at high and cold area
CN102613078A (en) * 2012-03-27 2012-08-01 浙江师范大学 Method suitable for rapid rooting of blueberry tissue culture seedlings

Also Published As

Publication number Publication date
CN103636373A (en) 2014-03-19

Similar Documents

Publication Publication Date Title
CN103636373B (en) One utilizes fresh and alive bog moss to carry out vegetative method to genus vaccinium plant
CN101401524B (en) Cutting propagation method for beach plum epicormic branch
CN103688835B (en) Strawberry potted tray cutting seedling method
CN101874447B (en) Beach plum root cutting seedling method
CN103583212B (en) The method of carnation seedling cave dish cutting propagation
CN105454018A (en) Cultivation method for echeveria succulent plants
CN103168609B (en) Pleione seed original habitat natural sowing method
CN106416900A (en) Planting method for promoting production increase at earlier stage of sweet cherries
CN106416809A (en) Koelreuteria paniculata hardwood cutting quick-seedling method
CN106718115A (en) A kind of apocarya breeding improves engrafting method
CN103931473A (en) Efficient and fast propagation method of white-flower common melastoma herbs
CN103563665B (en) A kind of formative method realizing alpine rose ancient tree stake one tree and spend more
CN101697703B (en) Method for bickiron inoculation of buds symbiotic to root fungi for culturing seedlings of Chinese chestnut
CN102349430A (en) Method for breeding seedlings of grapes by using containers
CN105594540A (en) Camellia pubipetala grafting and seedling cultivating method
CN107667759A (en) A kind of implantation methods of natural organic white tea
CN102893847A (en) Seedling raising substrate and cutting propagation method of bilberry plants
CN109168689B (en) Soilless cutting substrate and cutting method for great nanchuan tree tea
CN104782455B (en) Matrix and its application for tobacco axillary bud cutting propagation
CN107125120A (en) A kind of Chinese rose cuttage breeding method
CN105638371B (en) A kind of green propagation method of Gu tea tree
CN105660286B (en) A method of cultivating eggplant tree using radical bud induction graft technology
CN109197193A (en) A kind of method of U.S.'s autumn flame Acer palmatum ' Atropurpureum' rapid cuttage breeding
CN105993522A (en) Method for improving tulip tree cutting rooting survival rate
CN109220261A (en) A kind of dove tree seedling breeding method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model