CN105754880A - Nutrient solution for hydroponics plants capable of continuously releasing negative ions - Google Patents

Nutrient solution for hydroponics plants capable of continuously releasing negative ions Download PDF

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Publication number
CN105754880A
CN105754880A CN201610241314.XA CN201610241314A CN105754880A CN 105754880 A CN105754880 A CN 105754880A CN 201610241314 A CN201610241314 A CN 201610241314A CN 105754880 A CN105754880 A CN 105754880A
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nutritional solution
nutrient solution
plant
hydroponics
anion
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CN105754880B (en
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杨志坚
郑金贵
吴仁烨
许明
刘江洪
曹晓华
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
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  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)
  • Hydroponics (AREA)

Abstract

The invention relates to nutrient solution for hydroponics plants capable of releasing negative ions, and belongs to the field of functional plant hydroponics.The negative ions can be continuously released by the hydroponics plants such as tequila by the aid of the nutrient solution, and the lives of the hydroponics plants can be prolonged by the aid of the nutrient solution.The nutrient solution is improved Hoagland nutrient solution with piriformospora indica fungi.The nutrient solution has the advantages that the hydroponics plants (including the tequila and yucca gloriosa) can be cultivated by the aid of soilless cultivation methods, the nutrient solution can be applied to the hydroponics plants on plant negative ion machines, accordingly, the negative ions can be continuously and efficiently released, growth of the root systems of the hydroponics plants can be promoted, the leaves of the hydroponics plants keep deeply green, the negative ions can be assuredly continuously and efficiently released by the hydroponics plants in the plant negative ion machines, and the lives of the plants can be prolonged.

Description

A kind of nutritional solution making hydroponic plant sustained release anion
Technical field
The present invention relates to the nutritional solution of a kind of hydroponic plant anion releasing, belong to functional type water culture field.
Background technology
Anion is referred to as the vitamin in the middle of air, auxin and long-lived element, the health of human body is highly beneficial, but we can only breathe the anion air of indoor every cubic centimetre about 500 at current daily life, ten thousand anion in every cubic centimetre 1.9 to 12.1 of the scenic spots such as Wuyi Mountain, fujian, utilize plant anion machine that people can be made to breathe the anion air of every cubic centimetre about 2.6 at indoor 1 meter of, be equivalent to the air of approximate Forest Park.
Stimulating the root of plant to make plant persistently efficient anion releasing currently with plant anion machine, occur in that three kinds of situations while Plant emission anion, 1. most plant anion concentration all has no change;2. the content of anion raises to some extent, but plant is quickly old and feeble, blade is withered, even death;3. small part plant is only had can to continue the plant of efficient anion releasing, such as 18 kind of plant such as Folium Agaves variegatae, Yucca gloriosa, and these plants need specific nutrition liquid to carry out maintenance, otherwise the amount of anion releasing also can constantly reduce, life-span is also very short can only maintain about 180 days, and blade tip begins to wither.
Summary of the invention
It is an object of the invention to provide a kind of specific nutrition liquid making the hydroponic plant energy sustained release anion such as Folium Agaves variegatae and extending its life-span.
The nutrient solution prescription of the present invention is the modified form Glan nutritional solution suddenly containing India pyriform spore fungus.
Described modified form Glan nutritional solution suddenly is the Huo Gelan nutritional solution of 1/4 times of concentration.
Wherein India's pyriform spore (Piriformosporaindica, Pi) has promotion plant growing raising, promotes Nutrient Absorption, has the effects such as resistance.
The preparation of pyriform spore bacterium stock solution: the truffle that activated accesses in modified form Glan fluid medium suddenly, 28 DEG C, after 200r/min cultivates 7d, obtain mycelium stock solution.During process, pyriform spore mycelium stock solution is diluted 10 times, every strain Folium Agaves variegatae imposes 10ml diluent.
The present invention also protects the application of described nutritional solution, is that described nutritional solution soilless culture method is cultivated hydroponic plant, makes the plant sustained release anion being applied on plant anion machine.
Wherein said hydroponic plant includes Folium Agaves variegatae, Yucca gloriosa.
This nutritional solution soilless culture method cultivates hydroponic plant (including Folium Agaves variegatae, Yucca gloriosa), make the hydroponic plant persistently efficient anion releasing being applied on plant anion machine, promote root growth, keep leaf dark green, ensure that it can continue efficient anion releasing in plant anion machine, extend the life-span of plant.
Accompanying drawing explanation
Fig. 1 is the chlorophyll content of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 2 is the activity of the SOD of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 3 is the activity of the POD of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 4 is the activity of the CAT of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 5 is total MDA activity of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 6 is the root growth length of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated;
Fig. 7 is the anion burst size of the Folium Agaves variegatae that variable concentrations nutritional solution is cultivated.
Detailed description of the invention
Embodiment 1
Plant source anion generator is by Fujian agriculturalCrop science institute of university carriesFor (Wang Yanying etc., the research of plant source anion generator indoor application, Guangzhou University's journal (natural science edition), 13rd volume the 1st phase, 2014), houseplant chooses the Folium Agaves variegatae (Agaveamericanavar.marginata) of Agavaceae Agave, India pyriform spore (Piriformosporaindica, Pi) (Zhang Wenying, India pyriform spore fungus promotes that Semen Sesami grows and improves Semen Sesami drought resistance).
Process of the test:
1. the truffle activated accesses Huo Gelan fluid medium.In (Huo Gelan nutrient solution prescription: four water calcium nitrite 945mg/l, potassium nitrate 506mg/l, ammonium nitrate 80mg/l, potassium dihydrogen phosphate 136mg/l, Magnesium sulfate heptahydrate 493mg/l), 28 DEG C, 200r/min cultivate after 7d, obtain mycelium stock solution.
2. join the Huo Gelan nutrient solution prescription of different quality concentration, concentration respectively 1 times, 1/2 times, 1/4,1/8,1/16 Huo Gelan nutrient solution prescription.
3. in the Huo Gelan culture fluid of variable concentrations, add pyriform spore bacterium process group, during process, pyriform spore mycelium stock solution is diluted 10 times, every strain Folium Agaves variegatae imposes 10ml diluent.
4. the Folium Agaves variegatae original soil root dug out in ground is all cut, wash clean wound, wound disinfection is carried out with 1000 times of liquid of 50% thiophanate methyl, dry 1 day, allow and its base portion is soaked 10 seconds in the IAA of 20ppm, after drying again, after putting it into improvement, the Huo Gelan nutrition complex culture medium of variable concentrations carries out water planting.
5. measure the growing state of root after cultivating 7 days, 14 days, 21 days respectively, and measure the length of root.
6. after cultivating 21 days, Folium Agaves variegatae is put into the content of plant anion its anion of aircraft measurements.
7. Huo Gelan nutritional solution compound prescription is changed once for 3 weeks.
8. being allowed to condition on plant anion machine sustained release anion measures its chlorophyll, SOD, POD, MDA, CAT after 3 months.
Result:
The present invention using modified 1/4 suddenly Glan nutritional solution and symbiosis pyriform spore bacterium compound prescription make the root system of Folium Agaves variegatae more sturdy, leaf dark green, anion concentration is high.As shown in figs. 1-7, chlorophyll content reaches 86.00spad, is 1.39 times that carry out cultivating with water;The activity of SOD reaches 146.37u/g, is 1.17 times that carry out cultivating with water;The activity of POD reaches 0.20U/mgport, is 1.43 times with 1 times and 1/16 times Huo Gelan compound nutritional formula of liquid cultivation;The activity of CAT reaches 99.07U/ml, is 16.47 times cultivated with 1 times of Huo Gelan compound criteria formula of liquid;Total MDA activity reaches 11.92nmol/ml, is 3.06 times of 1/8 times of Huo Gelan compound criteria formula of liquid cultivation;After cultivating one week, the root of Folium Agaves variegatae reaches 6.80cm, is 1.68 times of 1/16 times of Huo Gelan compound criteria formula of liquid cultivation;After cultivating two weeks, the root of Folium Agaves variegatae reaches 12.70cm, is 1.03 times of 1/16 times of Huo Gelan compound criteria formula of liquid cultivation;After cultivating three weeks, the root of Folium Agaves variegatae reaches 19.60cm, is 1.37 times of 1/16 times of Huo Gelan compound criteria formula of liquid cultivation;1 meter of every cubic centimetre of of anion burst size distance can reach 36.42 ten thousand anion, is utilize under 1 times of Huo Gelan nutritional solution and symbiosis pyriform spore bacterium compound prescription 2.67 times of anion releasing amount, is carry out directly cultivating anion releasing amount with water 2.04 times.The Huo Gelan compound nutritional formula of liquid of application variable concentrations cultivates Folium Agaves variegatae, and concentration is too high, and overnutrition occurs in the root of Folium Agaves variegatae, there is green paint in root, little growth of microorganism occurs, is unfavorable for indoor cultivation, carrying out cultivating Folium Agaves variegatae with pure water to bleach because of overnutrition blade, blade is thinning.Utilize 1/4 suddenly Glan composite nutrient-fluid cultivate Folium Agaves variegatae, energy sustained release anion on plant anion machine, and growth potential energy keeps good state (make the leaf dark green of Folium Agaves variegatae, blade tip be not easy to wither, root growth good), the life-span reaches more than 6 months.

Claims (5)

1. a nutritional solution, it is characterised in that: described nutritional solution is modified form Glan nutritional solution or the nutrition product suddenly containing India pyriform spore fungus.
2. nutritional solution according to claim 1, it is characterised in that: described modified form Glan nutritional solution suddenly is the Huo Gelan nutritional solution of 1/4 times of concentration.
3. nutritional solution according to claim 1, it is characterised in that: described compounding is: the truffle that activated accessed in modified form Glan fluid medium suddenly, 28 DEG C, after 200r/min cultivates 7d, obtain mycelium stock solution.
4. the application of the nutritional solution as according to any one of claim 1-3, it is characterised in that: described nutritional solution soilless culture method is cultivated hydroponic plant, makes the plant sustained release anion being applied on plant anion machine.
5. the application of nutritional solution according to claim 4, it is characterised in that: described hydroponic plant includes Folium Agaves variegatae, Yucca gloriosa.
CN201610241314.XA 2016-04-19 2016-04-19 A kind of nutrient solution making hydroponic plant sustained release anion Expired - Fee Related CN105754880B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109716940A (en) * 2019-01-25 2019-05-07 福建农林大学 A kind of method of indoor quickly breeding vine tea seedling

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103858685A (en) * 2014-03-13 2014-06-18 贵州中烟工业有限责任公司 Method for reducing lead content of tobacco leaves
CN103898040A (en) * 2014-03-13 2014-07-02 浙江大学 Nano-zinc oxide-based culture medium capable of promoting microbial growth
WO2015001575A1 (en) * 2013-07-05 2015-01-08 Amity University Plant growth promoting formulation of piriformospora indica and azotobacter chroococcum with talcum powder

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015001575A1 (en) * 2013-07-05 2015-01-08 Amity University Plant growth promoting formulation of piriformospora indica and azotobacter chroococcum with talcum powder
CN103858685A (en) * 2014-03-13 2014-06-18 贵州中烟工业有限责任公司 Method for reducing lead content of tobacco leaves
CN103898040A (en) * 2014-03-13 2014-07-02 浙江大学 Nano-zinc oxide-based culture medium capable of promoting microbial growth

Non-Patent Citations (2)

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MANOJ KUMAR等: "Antioxidant enzyme activities in maize plants", 《MICROBIOLOGY》 *
王凤让等: "印度梨形孢及其近似种Sebacina vermifera 促进番茄生长发育及磷吸收", 《浙江大学学报(农业与生命科学版)》 *

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109716940A (en) * 2019-01-25 2019-05-07 福建农林大学 A kind of method of indoor quickly breeding vine tea seedling

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