CN109452154B - Indoor cutting seedling method for hemp - Google Patents
Indoor cutting seedling method for hemp Download PDFInfo
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- CN109452154B CN109452154B CN201811330194.6A CN201811330194A CN109452154B CN 109452154 B CN109452154 B CN 109452154B CN 201811330194 A CN201811330194 A CN 201811330194A CN 109452154 B CN109452154 B CN 109452154B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention provides a hemp indoor cutting seedling method, which at least comprises the following steps: (1) matrix treatment: soaking the rock wool blocks in water with the pH value of 5.0-5.7; then soaking the mixture in a nitrogen-phosphorus-potassium compound nutrient solution with the pH value of 5.7-6.0; (2) cuttage: soaking the hemp cutting shoots in a rooting agent, inserting rock wool blocks, and keeping the humidity at 60-65%; the temperature is 22-24 ℃, and the light intensity is 55-65 mu mol/(m)2S), culturing seedlings indoors under the condition that the density ratio of the red light to the blue light is 0.55:1-0.63: 1; (3) management: spraying amino acid foliar fertilizer twice a day, wherein the concentration of the foliar fertilizer is 0.05-0.1%; supplementing water and nutrition in the matrix. The method for cultivating the hemp shortens the seedling cultivation time, the hemp starts to root after one week of cuttage, and the hemp can completely root in two weeks, so that the seedling cultivation purpose is achieved; the survival rate of the seedlings is high and can reach 100 percent; the plants are all green in the process of seedling culture; meanwhile, compared with a soilless rapid propagation method, the method greatly reduces the using amount of the nutrient solution.
Description
Technical Field
The invention relates to a hemp indoor cutting seedling method.
Background
Cannabis sativa (Cannabis sativa) belongs to the genus Cannabis (Cannabinaceae) of the family Cannabis. It is an annual herbaceous plant, and is mostly a male and female heteroplant. The hemp has important economic value and can be used for various aspects such as textile, food, medicine, building materials, paper making and the like; more and more researches prove that the cannabinoid has wide pharmacological action, is widely applied to the treatment of chronic diseases and has large market demand.
The industrial hemp propagation is an important link of the cultivation technology, and the propagation modes widely applied in production are sexual propagation and vegetative propagation; wherein the seed propagation and growth period is long and the plant is easy to generate diseases in the seedling stage; the cutting seedling method is simple to operate, low in cost, fast in seedling and regular in growth, can effectively solve the problem of large individual difference of seed propagation, and is an optimal method for quickly obtaining hemp seedlings with high content and consistent characteristics.
In conventional cuttage, such as soil matrix cuttage propagation and novel soilless rapid propagation method, cutting slips start to root after half a month, and seedlings are lifted and transplanted after one month. About 100 cuttage seedlings can be obtained after 3 months, the seedling raising period is long, and the cuttage seedlings are planted outdoors, are easily influenced by the environment and have low survival rate; the seedling culture space requirement is large, the production quantity is small, and the soil-borne disease rate is high. The soilless rapid propagation method adopts a nutrient solution circulating system, the nutrient solution used for seedling culture is large in amount, the nutrient solution needs to be replaced in stages, and the soilless rapid propagation method has the defects of complex operation, low utilization rate of the nutrient solution, long seedling culture period and the like.
Disclosure of Invention
In view of the above disadvantages of the prior art, the present invention aims to provide an indoor cutting seedling method for hemp, which is used for solving the problems of long seedling time and low survival rate in the prior art.
In order to achieve the above and other related objects, the present invention provides a hemp indoor cutting seedling method, which at least comprises the following steps:
(1) matrix treatment: soaking the rock wool blocks in water with the pH value of 5.0-5.7; then soaking the mixture in a nitrogen-phosphorus-potassium compound nutrient solution with the pH value of 5.7-6.0;
(2) cuttage: soaking the hemp cutting slips in a rooting agent or a mixed solution of naphthylacetic acid and indoleacetic acid, inserting rock wool blocks, and keeping the humidity at 60-65%; the temperature is 22-24 ℃, and the light intensity is 55-65 mu mol/(m)2S), culturing seedlings indoors under the condition that the density ratio of the red light to the blue light is 0.55:1-0.63: 1;
(3) management: spraying amino acid foliar fertilizer twice a day, wherein the concentration of the foliar fertilizer is 0.05-0.1%; when the total mass loss of the substrate is 20-25%, if the cutting shoot does not root, the substrate is soaked in a nitrogen-potassium-phosphorus mixed fertilizer with the pH value of 5.7-6.0 and the EC value of 0-0.4 ms/cm, and if the cutting shoot roots, the substrate is soaked in a nitrogen-potassium-phosphorus mixed fertilizer with the pH value of 5.7-6.0 and the EC value of 0.4-1.0 ms/cm
And (4) adding nitrogen, potassium and phosphorus mixed fertilizer, and then continuously culturing to obtain the seedling.
Further, the cutting box containing the rock wool blocks is sterilized before the rock wool blocks are processed in the step (1). The disinfection can be carried out by the disinfection method in the prior art, for example, the disinfection method is cleaning by using disinfectant water, and 500ppm hypochlorous acid can be selected as the disinfectant.
Further, the water soaking time in the step (1) is 5-10 minutes, and the water is controlled for 1-2 minutes; the nutrient solution is soaked for 5-10 minutes, and water is controlled for 1-2 minutes. The pH of the water may be adjusted to 5.0 to 5.7 by adding an acid to the water, and for example, 0.5% hydrochloric acid, nitric acid, acetic acid, or other inorganic acid may be used.
Further, the nutrient solution in the step (1) is a nitrogen-phosphorus-potassium composite nutrient solution, wherein the mass ratio of nitrogen to phosphorus to potassium is 12-15: 7-15: 8-15. The nutrient solution can be purchased in the market or prepared by self, for example, quick-acting ternary compound fertilizer, Meile universal concentrated liquid fertilizer or 1:1 potassium dihydrogen phosphate: ammonium nitrate and the like are adopted in the mass ratio.
Further, in the step (2), a fluorescent lamp or an LED lamp is used for illumination.
Further, the length of the hemp cutting shoot selected in the step (2) is 10cm-17cm, the diameter is more than or equal to 2.0mm, the leaves are trimmed, so that the cutting shoot has three pairs of true leaves, and the tail end of the cutting shoot is cut into an inclined plane of 30-60 degrees.
Further, the branch of the cutting shoot adopts a twig with a single bud, and the twig can be lignified or semi-lignified.
Further, the rooting agent in the step (2) is any one or more of trypan, flourishing root powder or rapid root growth liquid, and the soaking time is 30-60 seconds. The rooting agent mainly comprises the components of indbutyl-naphthylacetic acid. The rooting agent produced by the three manufacturers is added with water to prepare a solution if the rooting agent is solid, and water can be added or not added if the rooting agent is liquid, so long as the concentration of an effective substance of the solution of the rooting agent is 500-800 mg/L. For example, ABT produced by guang agrichemical corporation of the four kingdom countries can be used as it is.
Furthermore, the concentration of the naphthylacetic acid in the mixed liquid of the naphthylacetic acid and the indoleacetic acid is 350-450 mg/L, and the concentration of the indoleacetic acid is 80-120 mg/L. Furthermore, the soaking time in the mixed solution of the marijuana cutting naphthylacetic acid and the indoleacetic acid is 90-150S.
Further, the step (2) of soaking the hemp cutting slips in the rooting agent further comprises the steps of dipping the hemp cutting slips in the rooting agent containing rooting powder gel, and then inserting the rooting powder gel into rock wool blocks.
Further, the Gel in step (2) is Glonex Rooting Gel-clone Gel.
Further, the depth of the hemp cutting slips inserted into the rock wool blocks in the step (2) is 1.5-2 cm; the row spacing of the cutting is 5-10 cm, and the planting spacing is 2-5 cm.
Further, the time for soaking the substrate in the step (3) in the mixed fertilizer is 10-20 seconds.
Further, the amino acid foliar fertilizer can adopt a foliar fertilizer of commercially available common amino acid. Such as fish peptide chitinase, carbohydrase fish protein, and spray green.
Further, the mass ratio of nitrogen, phosphorus and potassium in the nitrogen, phosphorus and potassium mixed fertilizer used in the step (3) is 12-15: 7-15: 8-15. The nitrogen-phosphorus-potassium compound fertilizer can be prepared by self or can be obtained commercially, such as 1:1 quick-acting ternary compound fertilizer, Meile-particle general concentrated liquid fertilizer or 1:1 monopotassium phosphate, ammonium nitrate and the like.
Furthermore, the management in the step (3) also comprises the step of placing each rock wool block on a shelf, so that the purpose of saving space is achieved.
Further, the method also comprises the following step (4) of hardening seedlings: transferring the seedlings into a culture medium, keeping the temperature and the humidity, and keeping the illumination intensity at 100-150 mu mol/(m)2S) and culturing for 2-6d while keeping the density ratio of the red light to the blue light to the luminous flux of 0.75: 1-1.50: 1.
If the seedlings need to be cultured in soil subsequently, the rockwool blocks can be removed, and the seedlings are transferred to a culture medium for continuous culture; if the rock wool blocks need to be cultured continuously subsequently, the rock wool blocks do not need to be removed.
Furthermore, the indoor culture forming mode in the method can adopt plastic greenhouses, brick wall pots, factory rooms and household rooms.
As mentioned above, the indoor cutting seedling method for the hemp has the following beneficial effects:
the seedling raising time is shortened, the root starts after one week of cuttage, and the root can be completely raised in two weeks, so that the seedling raising purpose is achieved; the survival rate of the seedlings is high and can reach 100 percent; the plants are all green in the process of seedling culture; meanwhile, compared with a soilless rapid propagation method, the method greatly reduces the using amount of the nutrient solution.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
TABLE 1 Experimental materials
Example 1
(1) In the seedling raising room, a shelf with a height of about 70cm is prepared, the shelf can be installed in multiple layers, and a fluorescent lamp tube is installed on each layer. Maintaining the space humidity at 60%; the temperature is 22 ℃, and the illumination is 55 mu mol/(m)2·s)。
And cleaning the cuttage box by using sodium hypochlorite of 500ppm, and airing for later use.
Soaking rock wool block in RO water with pH of 5.5 for 5min, and controlling water for 1 min; soaking in nutrient solution (nitrogen phosphorus potassium mass ratio of 12: 8: 9) with pH of 5.7 for 5min, and controlling water for 1 min.
(2) Selecting strong single-bud twigs, wherein the length of the cutting shoot is 15m, and the thickness of the cutting shoot is equal to or larger than 2.0 mm; trimming leaves and keeping three pairs of true leaves of the cutting slips.
Cutting the cutting slips into a slope with the length of 13cm and the tail end of 45 degrees by using a blade, scribing the tail end of the slope, putting the cut cutting slips into 0.5% ABT by mass percent, soaking for 30s, and dipping for 30 s.
Inserting the cutting slips into the rock wool blocks, wherein the cutting depth is about 2cm, the cutting slips and the rock wool blocks are tightly combined, the row spacing of the cutting slips is 8.3cm, the plant spacing is 3.8cm, and a cover of a seedling raising box is covered in time during the cutting process so as to maintain the good state of the cutting slips. The total number of the strains was 200.
(3) During the cuttage period, the seedling raising box is kept open all the way and is always covered with a cover. Spraying amino acid foliar fertilizer twice every day, namely spraying green: the concentration of the foliar fertilizer is 0.05 percent;
on the 3 rd day of culture, (the cuttings do not root) rock wool blocks with 20% of total mass loss of the matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 8: 9) with the pH value being 5.7 and the EC value being 0.4ms/cm for 10 s;
on the 7 th day of culture, (cutting shoots have rooted) rock wool blocks with 20% of total mass loss of matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 8: 9) with the pH value being 5.7 and the EC value being 0.6ms/cm for 10 s;
on the 12 th day of culture (the cutting has rooted), the rockwool blocks with 20% of total mass loss of the matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 8: 9) with the pH value being 5.7 and the EC value being 1.0ms/cm for 10s, and then the seedlings are continuously cultured.
(4) Transferring the seedlings to a culture medium, keeping the temperature at 24 ℃ and the humidity at 65%, and irradiating with light at an intensity of 100 [ mu ] mol/(m)2S), maintaining the ratio of red to blue at 0.75:1, and culturing for 3 d.
The experimental results are as follows: the cutting shoots start to root at the 7 th day, 186 plants can survive at the 12 th day of cutting, and the rooting rate is more than 93%. Can survive 200 plants in the 14 th day, the rooting rate reaches 100 percent, the survival rate of 200 plants after transplantation is 100 percent. The plants are all green in the process of seedling culture.
Example 2
(1) In the seedling raising room, a shelf with a height of about 70cm is prepared, the shelf can be installed in multiple layers, and a fluorescent lamp tube is installed on each layer. Maintaining the space humidity at 62%; the temperature is 24 ℃, and the illumination is 58 mu mol/(m)2·s)。
And cleaning the cuttage box by using sodium hypochlorite of 500ppm, and airing for later use.
Soaking rock wool block in RO water with pH of 5.0 for 5min, and controlling water for 1 min; soaking in nutrient solution (nitrogen phosphorus potassium mass ratio of 15: 15: 15) with pH of 5.0 for 5min, and controlling water for 1 min.
(2) Selecting strong single-bud twigs, wherein the length of the cutting shoot is 10cm-15cm, and the thickness of the cutting shoot is equal to or larger than 2.0 mm; trimming leaves and keeping three pairs of true leaves of the cutting slips.
Cutting the cutting slips into inclined planes with the length of 10cm and the tail end of 55 degrees by using a blade, scribing the tail ends of the inclined planes, putting the cut cutting slips into ABT with the mass fraction of 0.7%, soaking for 45s, and dipping for 45 s.
Inserting the cutting slips into the rock wool blocks, wherein the cutting depth is about 1.5-2cm, the cutting slips and the rock wool blocks are tightly combined, the row spacing of the cutting slips is 5cm, the plant spacing is 5cm, and a cover of a seedling raising box is covered in time during the cutting process so as to maintain the good state of the cutting slips. The total number of the strains was 120.
(3) During the cuttage period, the seedling raising box is kept open all the way and is always covered with a cover. Spraying amino acid foliar fertilizer twice every day, namely spraying green: the concentration of the foliar fertilizer is 0.08 percent;
on the 4 th day of culture, (the cuttings do not root) rock wool blocks with 20% of total mass loss of the matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) with the EC being 0.1ms/cm and the pH being 5.8 for 10 s;
on the 8 th day of culture (the cutting has rooted), soaking rock wool blocks with 20% of total mass loss of the matrix in a nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) for 10s, wherein the EC is 0.4ms/cm, the pH is 5.8;
on the 12 th day of culture (the cutting has rooted), rock wool blocks with 20% of total mass loss of the matrix are soaked in a nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) for 10s, wherein the EC is 0.4ms/cm, the pH is 5.8.
(4) Transferring the seedlings to a culture medium, maintaining the temperature and humidity, and irradiating with light intensity of 100 μmol/(m)2S), maintaining the ratio of blue to red at 0.95:1, and culturing for 4 days.
The experimental results are as follows: the 6 th day of the cutting slip starts to root, 114 plants can survive at the 12 th day of cutting, and the rooting rate is more than 95%. 120 plants can survive at 13d, the rooting rate reaches 100%, and the survival rate of transplanting 120 plants is 100%. The plants are all green in the process of seedling culture.
Example 3
(1) In the seedling raising room, a shelf with a height of about 70cm is prepared, the shelf can be installed in multiple layers, and a fluorescent lamp tube is installed on each layer. Maintaining the space humidity at 65%; the temperature is 23 ℃, and the illumination is 65 mu mol/(m)2·s)
And cleaning the cuttage box by using sodium hypochlorite of 500ppm, and airing for later use.
Soaking rock wool block in RO water with pH of 5.7 for 5min, and controlling water for 1 min; soaking in nutrient solution (nitrogen phosphorus potassium mass ratio of 10: 9: 9) with pH of 5.7 for 5min, and controlling water for 1 min.
(2) Selecting strong single-bud twigs, wherein the length of the cutting shoot is 10cm-15cm, and the thickness of the cutting shoot is equal to or larger than 2.0 mm; trimming leaves and keeping three pairs of true leaves of the cutting slips.
Cutting the cutting slips into inclined planes with the length of 12cm and the tail end of 60 degrees by using a blade, scribing the tail ends of the inclined planes, putting the cut cutting slips into 0.6% ABT by mass percent, soaking for 60s, and dipping for 30 s.
Inserting the cutting slips into the rock wool blocks, wherein the cutting depth is about 1.5-2cm, the cutting slips and the rock wool blocks are tightly combined, the row spacing of the cutting slips is 10cm, the plant spacing is 2cm, and a cover of a seedling raising box is covered in time during the cutting process to maintain the good state of the cutting slips. The total number of strains was 118.
(3) During the seedling raising period, the seedling raising box is kept open all the way and is always covered with a cover. Spraying amino acid foliar fertilizer twice every day, namely spraying green: the concentration of the foliar fertilizer is 0.05 to 0.1 percent; the mass of the matrix was weighed with a scale.
On the 4 th day of culture, (the cuttings do not root) rock wool blocks with 20% of total mass loss of the matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) with the EC being 0.2ms/cm and the pH being 5.8 for 10 s;
on the 8 th day of culture (the cutting starts to root), soaking rock wool blocks with 20% of total mass loss of the matrix in a nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) for 10s, wherein the EC is 0.6ms/cm, the pH is 5.8;
on the 12 th day of culture (the cutting has rooted), rock wool blocks with 20% of total mass loss of the matrix are soaked in a nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 15: 15: 15) for 10s, wherein the EC is 0.6ms/cm, the pH is 5.8.
(4) Transferring the seedlings to a culture medium, maintaining the temperature and humidity, and irradiating with light intensity of 100 μmol/(m)2S), maintaining the ratio of blue to red at 1.50:1, and culturing for 6 d.
The experimental results are as follows: the cutting shoots start to root at the 6 th day, and 111 plants can survive at the 12 th day of cutting, so that the rooting rate is more than 94%. Can survive 118 plants in the 13 th day, the rooting rate reaches 100 percent, the survival rate of the transplanted 118 plants is 100 percent. The plants are all green in the process of seedling culture.
Example 4
(1) In the seedling raising room, a shelf with a height of about 70cm is prepared, the shelf can be installed in multiple layers, and a fluorescent lamp tube is installed on each layer. Maintaining the space at a humidity of60 percent; the temperature is 24 ℃, and the illumination is 56 mu mol/(m)2·s)。
And cleaning the cuttage box by using sodium hypochlorite of 500ppm, and airing for later use.
Soaking rock wool block in RO water with pH of 5.7 for 10min, and controlling water for 2 min; soaking in nutrient solution (nitrogen phosphorus potassium mass ratio of 10: 9: 9) with pH of 5.7 for 8min, and controlling water for 2 min.
(2) Selecting strong single-bud twigs, wherein the length of the cutting shoot is 17cm, and the thickness of the cutting shoot is equal to or larger than 2.0 mm; trimming leaves and keeping three pairs of true leaves of the cutting slips.
Cutting the cutting slips into a slope with the length of 15cm and the end of 45 degrees by a blade, scribing the end of the slope, and soaking the cut cutting slips in a mixed solution of 400mg/L of naphthylacetic acid and 100mg/L of indoleacetic acid for 120 s.
Inserting the cutting slips into the rock wool blocks, wherein the cutting depth is about 1.5-2cm, the cutting slips and the rock wool blocks are tightly combined, the row spacing of the cutting slips is 8cm, the plant spacing is 3cm, and a cover of a seedling raising box is covered in time during the cutting process so as to maintain the good state of the cutting slips. The total number of the strains was 138.
(3) During the seedling raising period, the seedling raising box is kept open all the way and is always covered with a cover. Spraying amino acid foliar fertilizer twice every day, namely spraying green: the concentration of the foliar fertilizer is 0.05 to 0.1 percent; the mass of the matrix was weighed with a scale.
On the 4 th day of culture, (the cuttings do not root) rock wool blocks with 20% of total mass loss of the matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 7: 8) with the EC being 0.3ms/cm and the pH being 5.7 for 10 s;
on the 8 th day of culture (the cutting has rooted), soaking rock wool blocks with 25% of total mass loss of the matrix in a nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 7: 8) with the EC being 0.5ms/cm and the pH being 5.7 for 10 s;
on the 12 th day of culture (the cuttings have rooted), rockwool blocks with 25% of total mass loss of matrix are soaked in nitrogen-potassium-phosphorus mixed fertilizer (the mass ratio of nitrogen to phosphorus to potassium is 12: 7: 8) with the EC being 0.8ms/cm and the pH being 5.7 for 10 s.
(4) Transferring the seedlings to a culture medium, maintaining the temperature and humidity, and irradiating with light at 150 μmol/(m)2S), maintaining the ratio of blue to red at 0.75:1, and culturing for 3 d.
The experimental results are as follows: the cutting shoot starts to root at the 7 th day, and can survive 117 plants at the 12 th day of cutting, and the rooting rate is more than 84%. Can survive 136 plants at 14 th day, the rooting rate reaches 98 percent, the survival rate of transplanting survival 136 plants is 100 percent. The plants are all green in the process of seedling culture.
The above examples are intended to illustrate the disclosed embodiments of the invention and are not to be construed as limiting the invention. In addition, various modifications of the methods and compositions set forth herein, as well as variations of the methods and compositions of the present invention, will be apparent to those skilled in the art without departing from the scope and spirit of the invention. While the invention has been specifically described in connection with various specific preferred embodiments thereof, it should be understood that the invention should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described embodiments which are obvious to those skilled in the art to which the invention pertains are intended to be covered by the scope of the present invention.
Claims (9)
1. An indoor cutting seedling method for hemp is characterized by at least comprising the following steps:
(1) matrix treatment: soaking the rock wool blocks in water with the pH value of 5.0-5.7; then soaking the mixture in a nitrogen-phosphorus-potassium compound nutrient solution with the pH value of 5.7-6.0;
(2) cuttage: soaking the hemp cutting slips in a rooting agent or a mixed solution of naphthylacetic acid and indoleacetic acid, inserting rock wool blocks, and keeping the humidity at 60-65%; the temperature is 22-24 ℃, and the light intensity is 55-65 mu mol/(m)2S), culturing seedlings indoors under the condition that the density ratio of the red light to the blue light is 0.55:1-0.63: 1;
(3) management: spraying amino acid foliar fertilizer twice a day, wherein the concentration of the foliar fertilizer is 0.05-0.1%; when the total mass loss of the substrate is 20-25%, if the cutting shoot does not root, soaking the substrate in a nitrogen-potassium-phosphorus mixed fertilizer with the pH value of 5.7-6.0 and the EC value of 0-0.4 ms/cm, if the cutting shoot has root, soaking the substrate in a nitrogen-potassium-phosphorus mixed fertilizer with the pH value of 5.7-6.0 and the EC value of 0.4-1.0 ms/cm, and then continuing to culture to obtain a seedling;
(4) hardening seedlings: transferring the seedlings into a culture medium, and keeping the temperature and the humidity at the illumination intensity of 100-150 mu mol/(m)2S), the density ratio of the red light to the blue light is 0.75: 1-1.50: 1, and the culture lasts for 2-6 d.
2. The indoor hemp cutting seedling method according to claim 1, wherein: and (2) disinfecting the cutting box containing the rock wool blocks before treatment of the rock wool blocks in the step (1).
3. The indoor hemp cutting seedling method according to claim 1, wherein: the water soaking time in the step (1) is 5-10 minutes, and the water is controlled for 1-2 minutes; the nutrient solution is soaked for 5-10 minutes, and water is controlled for 1-2 minutes.
4. The indoor hemp cutting seedling method according to claim 1, wherein: the mass ratio of nitrogen, phosphorus and potassium in the nitrogen, phosphorus and potassium composite nutrient solution in the step (1) is 12-15: 7-15: 8-15.
5. The indoor hemp cutting seedling method according to claim 1, wherein: the length of the hemp cutting shoot selected in the step (2) is 10cm-15cm, the diameter is more than or equal to 2.0mm, the leaves are trimmed, so that the cutting shoot has three pairs of true leaves, and the tail end of the cutting shoot is cut into an inclined plane of 30-60 degrees.
6. The indoor hemp cutting seedling method according to claim 1, wherein: the rooting agent in the step (2) is selected from one or more of trypan, root-enhancing rooting powder or rapid rooting solution, and the soaking time is 30-60 seconds.
7. The indoor hemp cutting seedling method according to claim 1, wherein: and (3) after the hemp cutting is placed in the rooting agent for soaking in the step (2), dipping rooting gel, and then inserting the rooting gel into rock wool blocks.
8. The indoor hemp cutting seedling method according to claim 1, wherein: the amino acid foliar fertilizer is selected from any one or more of fish peptide chitinase, carbohydrase fish protein or spraying green.
9. The indoor hemp cutting seedling method according to claim 1, wherein: the mass ratio of nitrogen, phosphorus and potassium in the nitrogen, phosphorus and potassium mixed fertilizer used in the step (3) is 12-15: 7-15: 8-15.
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| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN110463705A (en) * | 2019-09-04 | 2019-11-19 | 黑龙江汉美生工业大麻科技有限公司 | A kind of dedicated root-growing agent of industrial hemp cuttage and preparation method thereof |
| CN110583467A (en) * | 2019-10-16 | 2019-12-20 | 广东圣之禾生物科技有限公司 | Automatic aeroponic rapid rooting and planting system and method for cuttage plants |
| US11116157B2 (en) | 2019-12-30 | 2021-09-14 | Rockwool International A/S | Method of propagating a Cannabis cutting |
| CN116548192A (en) * | 2019-12-30 | 2023-08-08 | 洛科威有限公司 | Propagation method of cannabis sativa cuttings |
| CN110972918B (en) * | 2019-12-31 | 2021-09-28 | 云南省农业科学院经济作物研究所 | Water culture rooting method for cutting seedlings of medicinal industrial hemp |
| CN111837673A (en) * | 2020-07-02 | 2020-10-30 | 黑龙江润地汉麻科技有限公司 | Cuttage method for cannabis sativa in pharmaceutical industry |
| CN112438129A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Method for promoting industrial hemp rooting |
| CN112341262A (en) * | 2020-11-18 | 2021-02-09 | 福建省中科生物股份有限公司 | Hemp water culture nutrient solution and application thereof |
| CN112438195A (en) * | 2020-11-18 | 2021-03-05 | 福建省中科生物股份有限公司 | Industrial hemp water culture cuttage method |
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| US9622426B2 (en) * | 2013-10-04 | 2017-04-18 | Charles E. Ankner | Method of improving the growth and production output of plants of the family Cannabaceae sensu stricto |
| CN107365202A (en) * | 2017-08-22 | 2017-11-21 | 宣城市乐丰农业技术服务有限公司 | One implantation methods to grow tobacco |
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