CN104521539B - Gold leaf acer negundo twig cutting seedling method - Google Patents

Gold leaf acer negundo twig cutting seedling method Download PDF

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Publication number
CN104521539B
CN104521539B CN201510026553.9A CN201510026553A CN104521539B CN 104521539 B CN104521539 B CN 104521539B CN 201510026553 A CN201510026553 A CN 201510026553A CN 104521539 B CN104521539 B CN 104521539B
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cutting
gold leaf
cuttage
acer negundo
leaf acer
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CN104521539A (en
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黄成林
张宁
荆天
李春生
胡天培
朱文玲
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Zhonghui ecological environment Co., Ltd.
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Anhui Agricultural University AHAU
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention discloses a gold leaf acer negundo twig cutting seedling method which comprises the steps of cutting bed preparation, cutting stem selection, cutting shoot making, cutting shoot hormone rooting induction, cutting, post-cutting management and the like. Perlite is adopted as a substrate of a cutting bed, and a magnetic field network, an electric field network and an automatic atomizing system are arranged; cutting stems are selected within May, June and July; cutting stems with the diameter ranging from 4 mm to 6 mm are selected as cutting shoots and soaked in a growth regulating agent for 1 min to 2 h; a straight cutting method is adopted for cutting the cutting shoots into the seedbed, the automatic atomizing system carries out automatic atomization after cutting is finished when the air humidity is smaller than 90%; in the period from cutting to callus forming, the shading rate is kept 75%, the relative humidity is kept from 90% to 91%, and the temperature is kept from 30 DEG C to 40 DEG C; in the middle and later periods of cutting, the air humidity is controlled to range from 70% to 80%, the temperature is controlled to range from 30 DEG C to 40 DEG C, and the cutting shoots are processed by 100000 V voltage for 2 h every day. According to the gold leaf acer negundo twig cutting seedling method, the cutting environment most suitable for growth of gold leaf acer negundo twigs is obtained through screening by adjusting and controlling cutting shoot obtaining time, the cutting environment, the cutting shoot selection, the cutting substrate selection, the selection of hormone promoting rooting and other aspects, and the method is stable and efficient for gold leaf acer negundo twig cutting propagation.

Description

A kind of gold leaf Acer negundo. L epicormic branch cutting method
Technical field
The invention belongs to technical field of plant asexual propagation is and in particular to gold leaf Acer negundo. L epicormic branch cuttage technology.
Background technology
Gold leaf Acer negundo. L (acer negundo ' kellys gold ') is the Aceraceae Acer L high megaphanerophyte of fallen leaves, is north The variety of U.S. Acer negundo. L (acer negundo), also known as leaf maple, ash-leaved maple, original North America, in North America, Europe extensively For urban afforestation.Russia has plants and is used for build shelter-forest in a large number, and the end of the eighties in last century has just introduced China, tree crown Ball-shaped, sprig is smooth, often by white wax powder, imparipinnate leafs opposite, very big 5-7 piece of lobule, leaf color is soft, and blade is whole The individual season of growth shows as yellow, spring bright yellow, fades to yellow green, summer not burnt leaf, raceme, flower no lobe;Infructescence Sagging, two wings are carried out at an acute angle, and May elder generation's leaf is bloomed, and September fruit is ripe.
Light, happiness Cold and cool climate, resistance to -40 DEG C~-45 DEG C low temperature, drought-enduring, waterlogging, less demanding to soil property, no matter fertile lean Or soda acid soil all can grow, adaptable, cold tolerance is strong, has wider Bioclimatic analysis, pest and disease damage is few, is that city is beautiful The beautiful ornamental tree species of change, green barren hill, color.Spring and summer autumn green dry, Flos Carthami is graceful and bold and unrestrained;Winter green dry become red dry, Warm and ardent, it is described as " the princess tree " in shade tree.Preferably make shade tree, shade tree, specimen tree, flourishing it is easy to shape Become large-area colony landscape, there is the very big market demand, be the important middle level trees showing autumn scenery in scenic forest, numerous Autumn scenery Colored- leaf Plants in, gold leaf Acer negundo. L is taken the course of its own, great glamour.Not only leaf color is golden yellow bright for gold leaf Acer negundo. L, and Increment is big, is rare high megaphanerophyte in Colored- leaf Plants family.
Gold leaf Acer negundo. L seed sowing variability is big, is unfavorable for ensureing the merit of this kind, current gold leaf Acer negundo. L Breeding adopts propagation by grafiting, but propagation by grafiting is higher to technical requirements, will cultivate masterful technique work, and is limited by stock seeds System, the cultivation of stock also will expend certain human and material resources, and overall reproductive speed is slow, relatively costly.
Epicormic branch cuttage is widely used as a kind of technology of suitable scale high efficiency quick breeding, has rapid appearance of taking root Easily, the advantages of well developed root system, survival rate height, fringe bar abundance, cycle is short of emerging, and maternal plant can completely be retained Institute is advantageous, can keep well continuing color tree species leaf color character, be well suited for the breeding for color tree species in gardens.Open Exhibition gold leaf Acer negundo. L epicormic branch cuttage, and cutting collection is combined with the pruning of summer sparse branching, the branch removing can be turned waste into wealth, Utilize once again, the breeding and Seedling Industrialization Development promoting gold leaf Acer negundo. L is had great importance.
Research conclusion with regard to gold leaf Acer negundo. L epicormic branch cuttage is inconsistent, and the index weighing growth is excessively single, about grinding Study carefully result it is also shown that Aceraceae plant is seeds difficult to take root, containing internal hormones and related enzyme such as tannin, belong to and induce the life of root base Root type.
Content of the invention
The problem that the present invention need to solve is not enough for prior art, is promoted using advanced high-pressure electrostatic magnetization seedling-raising technique Enter cuttage rooting, simultaneously from cuttage environment, hormone kind, hormone concentration, cutting time, adopt in terms of fringe portion position etc. to gold leaf compound leaf Maple cutting propagation carries out system regulation, to screening the optimum condition of gold leaf Acer negundo. L softwood cutting propagation, is gold leaf Acer negundo. L Softwood cutting propagation provides a kind of method of stability and high efficiency.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of gold leaf Acer negundo. L epicormic branch cutting method, its feature is, comprises the steps:
(1) slotting machine prepares:
Slotting machine is located in temperature control green house, is substrate using perlite, paving 8-10cm thickness perlite, its lower laying Magnet, Make sealing coat with non-woven fabrics between Magnet and perlite, at 40cm above slotting machine, lay upper electric field net, the lower electricity of magnetic underlayer laying Net, upper electric field side off the net lays automatic spraying system;
(2) cutting is chosen
Select the cloudy day, the raw then twig taking robust growth on gold leaf Acer negundo. L maternal plant, no disease and pests harm, as cutting, is inserted Bar access time is 5-7 month;
(3) cutting makes:
Select diameter 4mm-6mm cutting as cutting, each cutting is cut into 10cm-15cm, retain 2-3 section, upper end exists It is cut into flat mouth, lower end is truncated at the lower 0.5cm of section, and otch smooths at 2cm on bud;Each cutting retains less by one in the compound leaf of side / 2nd blades of piece;By described cutting soaking disinfection 15 minutes;
(4) the hormone root induction of cutting and cuttage:
Cutting bottom after disinfecting soaks 1min-2h in 50-800ppm growth regulator;Using straight cutting method skewer Insert in the slotting machine described in step (1), inserting soil depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, line-spacing 7cm;
(5) manage after cuttage:
In temperature control green house, from cuttage to Callus formation during, keep shading rate 75%, relative humidity 90-91%, 30 ℃-40℃;By simulating blade sensor, as air humidity < 90%, start auto spraying, reach 90- to relative humidity Stop spraying when 91%;Cuttage middle and late stage is then to be gradually lowered air humidity to the phase of taking root after Callus formation, controls air Humidity is in 70%-80%, 30 DEG C -40 DEG C of temperature;It is equipped with high-voltage generator, by upper and lower electric field net daily with 100,000 volts simultaneously Voltage is processed 2 hours to cutting.
Described cutting time is late July.
The growth regulator of described step (4) is iba, and concentration is 50ppm, and soak time is 2h.
The present invention having the beneficial effect that with respect to prior art
Cuttage and seedling culture method of the present invention, accelerates gold leaf Acer negundo. L twig and takes root, and promotes cutting growth promoter, not only shortens Growing-seedling period, makees cutting in combination with the useless branch cut during using summer training or grafting, reduces cost, be conducive to gold leaf Acer negundo. L cutting propagation industrialization is carried out.
Cottage method of the present invention, takes root in two weeks, rooting rate reaches 81.11% in a large number, the cutting survival rate after taking root 93.4%.
Electrostatic field of the present invention produces micro-current potential difference under elevated pressure conditions, makes cellular endogenous auxin from electronegative potential to height Current potential transport, negative ions move, break cutting in vitro after of short duration dormancy.Potential difference is the important dynamic of Polar Transport of Auxin Power, on the one hand because transmembrane potential increases, makes hormone transmit faster, after living cells damaged, current of injury produced by it Strengthen therefrom, promote information and the transmission of material, so that injury is produced and repair responsing reaction, thus promoting cutting wound healing Organize the formation of.Another aspect cell membrane carries out the important channel of mass exchange as cell and surrounding physiological environment, has extremely Crucial effect.When in certain electrostatic field to plant, cell membrane can be made excited, improve the permeability of ion, and then promote Enter the process that cell absorbs ion, promote cutting cells in vivo to break up, improve the speed of the mass exchange inside and outside cell membrane, quickly It is converted into cell of taking root, the activity of activated in advance enzyme, accelerates metabolism, improve photosynthetic rate and the breathing rate of cutting blade Thus promoting cutting internal water and the conveying activity of nutrient.
Specific embodiment
Below by way of specific embodiment, explanation is further explained to technical solution of the present invention.
(1) seedbed prepares:
Experiment is carried out in the intelligent temperature control booth of Hefei forestry Advanced Technology Center, booth area about 2000, totally 98 Seedbed.It is substrate using perlite, seedbed specification is 10.0m × 1.2m × 0.4m, for ease of operation and slotting machine draining, in seedbed The heart is higher than both sides 2cm, and the bottom will reserve osculum.Tiling 10cm thickness perlite on seedbed, its lower laying Magnet, in Magnet and Make sealing coat with non-woven fabrics between perlite, at 40cm above slotting machine, lay upper electric field net, the lower electric field net of magnetic underlayer laying, on Electric field side off the net lays automatic spraying system, is equipped with simulation blade sensor, is sprayed water according to slotting machine moisture situation, is equipped with high simultaneously Voltage generator, daily 100,000 volts of low pressure are processed 2 hours to cutting.
(2) cutting is chosen:
Select the cloudy day to take the raw then twig of robust growth on maternal plant, no disease and pests harm, choose hypomere lignifying in branch The high part of degree does cutting.And respectively in May 24, June 24, July 24 respectively adopted a cutting and carries out cutting time examination Test contrast.
(3) cutting makes:
According to prior art, cutting is cut into 10cm-15cm (retain 2~3 section), the cuttage branch of a diameter of 4mm-6mm, Upper end is cut into flat mouth at 2cm on bud, and lower end is truncated at the lower 0.5cm of section, and otch smooths.Thickness according to cutting selects reservation one Less 1/2nd a piece of blades in the compound leaf of side.Cutting 30 is a bundle, puts in 25% carbendazol wettable powder and soaks Sterilization 15 minutes.And select cutting top, middle part, bottom to carry out contrast test, substrate, hormone, cutting time as cutting respectively The test that gold leaf Acer negundo. L cutting propagation is affected is all using cutting in the middle part of branch.
(4) the hormone root induction of cutting and cuttage:
External source root-promoting hormone is abt1 root-inducing powder, naphthalene acetic acid (naa), indolebutyric acid (iba), heteroauxing (iaa), naa+ Need first to add auxiliary solvent during iba (isoconcentration mixing), abt1 dissolving, then use warm water solution.Naphthalene acetic acid (naa), indolebutyric acid (iba) and heteroauxing (iaa) dissolving when need to add a little sodium hydroxide hydrotropy.Cutting bottom after disinfecting exists 2h is soaked in 50ppm, 100ppm, 200ppm growth regulator;Dip 1min in 500,800ppm growth regulator, solution does not have Cross base portion 3cm, every 30 cutting are one group, experiment is processed in triplicate and with clear water as comparison, carries out root-promoting hormone and dense The contrast test of degree.The test that cutting position, cutting time, cuttage environment affect on gold leaf Acer negundo. L cutting propagation all adopts iba50ppm.
Using straight cutting method, before cuttage, substrate is poured permeable, cuttage immediately under moisture state after HORMONE TREATMENT, light after cuttage Light press cutting surrounding substrate, is allowed to be fully contacted with cutting, and inserting soil depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, OK Away from 7cm.Cuttage sprays 400 times of liquid of 25% carbendazol wettable powder after terminating, and prevents fungal infection cutting.
(5) manage after cuttage:
In temperature control green house, from cuttage to Callus formation during, keep shading rate 75%, relative humidity 90-91%, 30 ℃-40℃;By simulating blade sensor, as air humidity < 90%, start auto spraying, reach 90- to relative humidity Stop spraying when 91%;Cuttage middle and late stage is then to be gradually lowered air humidity to the phase of taking root after Callus formation, controls air Humidity is in 70%-80%, 30 DEG C -40 DEG C of temperature;It is equipped with high-voltage generator, by upper and lower electric field net daily with 100,000 volts simultaneously Voltage is processed 2 hours to cutting.Culture can become transplantation of seedlings in one month.
The present embodiment relevant experimental data is shown in Table 1-3.
The impact that the process of table 1 hormon is taken root to gold leaf Acer negundo. L epicormic branch cuttage
The different cutting time of table 2 is to gold leaf Acer negundo. L epicormic branch cuttage Rooting effect
The different cutting position of table 3 is to gold leaf Acer negundo. L epicormic branch cuttage Rooting effect

Claims (1)

1. a kind of gold leaf Acer negundo. L epicormic branch cutting method is it is characterised in that comprise the steps:
(1) slotting machine prepares:
Slotting machine is located in temperature control green house, is substrate using perlite, paving 8-10cm thickness perlite, and its lower laying Magnet, in Magnet Make sealing coat with non-woven fabrics and perlite between, at 40cm above slotting machine, lay upper electric field net, the lower electric field net of magnetic underlayer laying, Upper electric field side off the net lays automatic spraying system;
(2) cutting is chosen
Select the cloudy day, the raw then twig taking robust growth on gold leaf Acer negundo. L maternal plant, no disease and pests harm as cutting, select by cutting The time is taken to be 5-7 month;
(3) cutting makes:
Select diameter 4mm-6mm cutting as cutting, each cutting is cut into 10cm-15cm, retain 2-3 section, upper end is on bud It is cut into flat mouth, lower end is truncated at the lower 0.5cm of section, and otch smooths at 2cm;Each cutting retains less a piece of in the compound leaf of side / 2nd blades;By described cutting soaking disinfection 15 minutes;
(4) the hormone root induction of cutting and cuttage:
Cutting bottom after disinfecting soaks 2h in 50ppmiba growth regulator;Using straight cutting method cuttage in step (1) slotting machine described in, inserting soil depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, line-spacing 7cm;
(5) manage after cuttage:
In temperature control green house, from cuttage to Callus formation during, keep shading rate 75%, relative humidity 90-91%, 30 DEG C- 40℃;As air humidity < 90%, start auto spraying, reach to relative humidity and during 90-91%, stop spraying;Cuttage middle and late stage Then it is gradually lowered air humidity to the phase of taking root after Callus formation, control air humidity in 70%-80%, 30 DEG C of temperature- 40℃;It is equipped with high-voltage generator simultaneously, by upper and lower electric field net daily 100,000 volts of voltages, cutting is processed 2 hours;
Described cutting time is late July.
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