CN109673290A - A kind of regenerated watermelon tip cutting engrafting method of reduction stock sprout tillers - Google Patents
A kind of regenerated watermelon tip cutting engrafting method of reduction stock sprout tillers Download PDFInfo
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- CN109673290A CN109673290A CN201910032154.1A CN201910032154A CN109673290A CN 109673290 A CN109673290 A CN 109673290A CN 201910032154 A CN201910032154 A CN 201910032154A CN 109673290 A CN109673290 A CN 109673290A
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- 241000219109 Citrullus Species 0.000 title claims abstract description 37
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 34
- DARPYRSDRJYGIF-PTNGSMBKSA-N (Z)-3-ethoxy-2-naphthalen-2-ylsulfonylprop-2-enenitrile Chemical compound C1=CC=CC2=CC(S(=O)(=O)C(\C#N)=C/OCC)=CC=C21 DARPYRSDRJYGIF-PTNGSMBKSA-N 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 7
- 230000003020 moisturizing effect Effects 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 229920006255 plastic film Polymers 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 230000008929 regeneration Effects 0.000 abstract description 8
- 238000011069 regeneration method Methods 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 238000007796 conventional method Methods 0.000 description 7
- 235000019441 ethanol Nutrition 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of regenerated watermelon tip cutting engrafting method of reduction stock sprout tillers, it includes stock nursery, scion nursery, grafting and management, belongs to crop-planting field.The present invention can effectively inhibit the regeneration of stock sprout tillers, reduce the number of artificial removal's sprout tillers and time after grafting, reduce labour's investment, the graft survival rate of watermelon can also be improved simultaneously, reduce nutrient competition, promote the growth of scion, and improve grafting quality, lays a good foundation to obtain high yield and high quality watermelon.
Description
Technical field
The invention belongs to crop-planting fields, are related to Grafting watermelon method, and especially a kind of reduction stock sprout tillers is regenerated
Watermelon tip cutting engrafting method.
Background technique
Watermelon is the important garden crop in China, is promoting increasing peasant income and is meeting the growing melon and fruit party in request of people
Face plays great function.In recent years, continuous cropping obstacle is got worse in cultivating watermelon, especially under Protected Cultivation, is withered
The soil-borne diseases such as disease aggravate, and influence the normal growth of watermelon plant, so as to cause yield reduction and quality decline, resistance
The development of watermelon industry is hindered.Grafting be overcome an effective measures of the above problem, thus watermelon production in obtained it is general
All over application.
The main method of Grafting watermelon is the method for plant propagation with cutting, cleft graft, inarching method etc. at present.Engrafting method of the present invention is
The method of plant propagation with cutting.Since after grafting, sprout tillers can occur repeatedly in grafting stock position, and remove and be more troublesome, waste of manpower object
Power.Mepiquat chloride is a kind of important growth regulatory substance, in view of the above-mentioned problems, the present invention develops a kind of inhibition Grafted Watermelon anvil
The wooden regenerated chemical method of sprout tillers, i.e., handled stock growing point by mepiquat chloride, and discovery can not only reduce stock and sprout
In addition tiller regeneration rate can also improve Grafting watermelon survival rate and improve seedling quality.
Summary of the invention
The object of the present invention is to provide a kind of regenerated watermelon tip cutting engrafting methods of reduction stock sprout tillers, it is intended to inhibit grafting
The regeneration of stock sprout tillers and raising Grafting watermelon survival rate afterwards.
The technical scheme adopted by the invention is that:
A kind of regenerated watermelon tip cutting engrafting method of reduction stock sprout tillers, comprising the following steps:
1) stock nursery: stock seed being carried out disinfection, is soaked seed, vernalization processing, is then seeded in hole tray, long to 1 leaf 1
For grafting when the heart；
2) scion nursery: watermelon ingraftment seed is carried out disinfection, is soaked seed, vernalization processing, is then broadcasted sowing in flat chassis, son
For grafting when Ye Gang is open and flat；
3) it grafts: first removing stock growing point before grafting, then drop 0.01-0.1ml mass concentration is at removal position
Scion is grafted on stock by the mepiquat chloride ethanol solution of 2-5% in 5 days by tip cutting method；
4) it manages: carrying out epidemic disaster and illumination management after grafting.
Preferably, the stock is cucurbit stock, more preferably the glad anvil in capital 1.
Preferably, the watermelon scion is early good 8424.
Preferably, the mass concentration of the mepiquat chloride ethanol solution is 3%.
Preferably, the temperature management is: 1-3d keeps 28-30 DEG C of daytime temperature, 23-25 DEG C of night temperature after grafting；4-6d daytime temperature
26-28 DEG C, 20-22 DEG C of night temperature；Thereafter temperature is gradually decrease to 22-25 DEG C of daytime temperature, 18-20 DEG C of night temperature as grafting grows；
When watermelon seedling length to 11 heart of leaf, night temperature is maintained at 16-18 DEG C.
Preferably, the moisture management is: total principle is " dry not wilt, wet not ponding "；1-3d after grafting, with moisturizing
Based on, but scion growing point should not ponding, can use plastic film wrap up grafting moisturizing；4-5d after grafting should gradually divulge information
Light, ventilation time is not wilted with scion cotyledon to be advisable；After 10d by seedbed management.
Preferably, the illumination management is: as long as scion is not wilted after grafting, should increase illumination as far as possible；Grafting
1-3d afterwards, daytime covers sunshade net shading when illumination is stronger, and early morning is appropriate at dusk light-exposed, and the time wants short；Gradually extend light after 3d
According to the time, it is not required to shade again after 1 week；When Winter-Spring nursery, such as low temperature sleet or successive cloudy days gas, appropriate light filling is answered.
The beneficial effects of the present invention are:
(1) the method for the present invention is used, in particular by mepiquat chloride (1,1- dimethyl piperidyl chloride) to stock
After growing point processing, it can effectively inhibit the regeneration of stock sprout tillers, sprout tillers regeneration rate reduces by 39.14% compared with conventional method, thus
Reduce the number of artificial removal's sprout tillers and time after grafting, reduces labour's investment.
(2) the method for the present invention is used, the graft survival rate of watermelon reaches 93.97%, improves 4.03% than conventional method.
(3) the method for the present invention is used, seedling vigorous index, root long, root surface area, root diameter, the root body of watermelon can also be increased
Long-pending and tip of a root number, illustrates that this method also can be reduced nutrient competition, promotes the growth of scion, and improve grafting quality, to obtain
High yield and high quality watermelon is obtained to lay a good foundation.
Detailed description of the invention
Fig. 1 is to graft stock respectively at 0,3,5 day using after conventional method removal growing point, and that observes after 24 days sprouts
Tiller regeneration effect figure, arrow is directed toward stock sprout tillers in figure.
Fig. 2 is to graft after handling stock growing point with mepiquat chloride respectively at 0,3,5 day, and the sprout tillers observed after 24 days is again
Effect picture is given birth to, stock sprout tillers quantity significantly reduces in figure.
Specific embodiment
The present invention will be described in detail combined with specific embodiments below.
Embodiment 1
Variety selection: the present embodiment chooses cucurbit stock " the glad anvil in capital 1 " and is used as graft stock, and variety of watermelon is " early good
8424 " be scion.
Culture facility disinfection: utensil used in nursery place, arched shed, canopy film, insulation quilt and entire production link and make
Used matrix uses 40% formaldehyde, 50 times of liquid spraying disinfections, dosage 30ml/m2, 48h is closed, then the 5d that divulges information waits for formaldehyde
Nursery work can be carried out after volatilization completely.
(1) stock nursery: stock seed being carried out disinfection, is then hoted water treatment of seeds seed, and soak seed 8-24h, then
Seed is rinsed and is dried, 30 DEG C or so vernalization, when radicle it is long to 0.5cm when be seeded in hole tray, it is long to 11 heart of leaf when transferred
It connects.
(2) scion nursery: watermelon ingraftment seed being carried out disinfection, is then hoted water treatment of seeds seed, and soak seed 6-8h；So
Seed is rinsed afterwards and is dried, 30 DEG C or so vernalization, when radicle it is long to 0.5cm when broadcast sowing in flat chassis, when cotyledon is just open and flat into
Row grafting.
(3) it grafts: when 11 heart of leaf of stock, removing growing point, drop 0.05ml (about one drop) mass concentration is at removal position
3% mepiquat chloride ethanol solution (ethyl alcohol for being 50% using mass concentration is solvent), was grafted onto anvil by tip cutting method for scion in 5 days
On wood, on the day before grafting, scion, stock matrix need to be sprinkled profoundly water.
The concrete operations of tip cutting method grafting are as follows:
Stock processing: insertion grafting label, it is at 45 ° to another cotyledon petioles base portion that grafting label are close to cotyledon petioles middle arteries base portion
Left and right oblique cutting, jack depth are that grafting label slightly penetrate brokenly stock hypocotyl cortex, and grafting label wouldn't be extracted；
Scion processing: cutting scion, scion seedling of choosing, and at 0.5-1.0cm below cotyledon base portion, a knife of splaying, inclined-plane is long
0.7-1.0cm；
Grafting: scion is inserted into stock, is extracted to graft to sign to splay scion and is injected stock jack downwards, interface is tight
Real, stock cotyledon and scion cotyledon are crossed as "+" shape.
(4) managing after grafting:
Temperature management: 1-3d daytime temperature is kept for 28-30 DEG C after grafting, 23-25 DEG C of night temperature.26-28 DEG C of temperature of 4-6d daytime, night temperature
20-22℃.Thereafter temperature management is gradually decreased as grafting grows, 22-25 DEG C of daytime temperature, 18-20 DEG C of night temperature.When watermelon seedling is long
When to 11 heart of leaf, night temperature is positively retained at 16-18 DEG C.As temperature is excessively high preferably with sunshade net cooling or appropriate ventilation drop during nursery
Temperature maintains the temperature at 32 DEG C or less.
Moisture management: total principle is " dry not wilt, wet not ponding ".1-3d after grafting, based on moisturizing, but scion is raw
Long point should not ponding.Grafting moisturizing can be wrapped up with plastic film.4-5d after grafting, should gradually ventilation and penetrating light, ventilation time with
Scion cotyledon does not wilt and is advisable.After 10d by seedbed management.
Illumination management: as long as scion is not wilted after grafting, illumination should be increased as far as possible.1-3d after grafting, illumination compared with
Daytime covers sunshade net shading when strong, and early morning is appropriate at dusk light-exposed, and the time wants short.Gradually extend light application time after 3d, after 1 week not
It need to shade again.When Winter-Spring nursery, such as low temperature sleet or successive cloudy days gas, appropriate light filling is answered.
Conventional method (control does not use mepiquat chloride):
When 11 heart of leaf of stock, artificial removal's stock growing point repeatedly removes stock sprout tillers after grafting survival, remaining process with
1 method of embodiment is identical.
Embodiment 1 is compared with the grafting index of correlation of direct tip cutting connection, the results are shown in Table 1.
1 the method for the present invention of table is compared with conventional method index of correlation
1 the method for the present invention of table (measures) for the 24th day after grafting compared with conventional method index of correlation
Note: seedling vigorous index: (stem thickness/plant height) × overground part dry weight refers at stock growing point mepiquat chloride for 0 day, 3 days, 5 days
To the time for starting grafting after reason.
The present invention handles stock growing point using mepiquat chloride, can effectively inhibit the regeneration of sprout tillers, and sprout tillers regeneration rate relatively passes
System method reduces by 39.14%, to reduce the number of artificial removal's sprout tillers and time after grafting, reduces the throwing of manpower and material resources
Enter；The graft survival rate of watermelon reaches 93.97%, improves 4.03% than conventional method；In addition, being compared with the traditional method, this hair
Bright seedling vigorous index, root long, root surface area, root diameter, root volume and tip of a root number increase, and Miao Gengzhuan illustrates that the present invention can also be effective
Nutrient competition is reduced, promotes the growth of scion, and improve grafting quality, is laid a good foundation to obtain high yield and high quality watermelon.
Claims (6)
1. a kind of regenerated watermelon tip cutting engrafting method of reduction stock sprout tillers, it is characterised in that the following steps are included:
1) stock nursery: stock seed being carried out disinfection, is soaked seed, vernalization processing, is then seeded in hole tray, it is long to 11 heart of leaf when
For grafting；
2) scion nursery: watermelon ingraftment seed is carried out disinfection, is soaked seed, vernalization processing, is then broadcasted sowing in flat chassis, cotyledon is rigid
For grafting when open and flat；
3) it grafts: first removing stock growing point before grafting, then drop 0.01-0.1ml mass concentration is 2-5% at removal position
Mepiquat chloride ethanol solution, scion is grafted on stock by tip cutting method in 5 days；
4) it manages: carrying out epidemic disaster and illumination management after grafting.
2. reducing the regenerated watermelon tip cutting engrafting method of stock sprout tillers as described in claim 1, it is characterised in that: the stock
It is cucurbit stock.
3. reducing the regenerated watermelon tip cutting engrafting method of stock sprout tillers as described in claim 1, it is characterised in that: the casing coupling
The mass concentration of amine ethanol solution is 3%.
4. reducing the regenerated watermelon tip cutting engrafting method of stock sprout tillers as described in claim 1, which is characterized in that the temperature
Management is: 1-3d keeps 28-30 DEG C of daytime temperature, 23-25 DEG C of night temperature after grafting；26-28 DEG C of 4-6d daytime temperature, 20-22 DEG C of night temperature；Thereafter
Temperature is gradually decrease to 22-25 DEG C of daytime temperature, 18-20 DEG C of night temperature with grafting growth；When watermelon seedling length to 11 heart of leaf, night temperature
It is maintained at 16-18 DEG C.
5. reducing the regenerated watermelon tip cutting engrafting method of stock sprout tillers as described in claim 1, which is characterized in that the humidity
Management is: total principle is " dry not wilt, wet not ponding "；1-3d after grafting, based on moisturizing, but scion growing point Ying Buji
Water can wrap up grafting moisturizing with plastic film；4-5d after grafting, should gradually ventilation and penetrating light, ventilation time with scion cotyledon not
Wilting is advisable；After 10d by seedbed management.
6. reducing the regenerated watermelon tip cutting engrafting method of stock sprout tillers as described in claim 1, which is characterized in that the illumination
Management is: as long as scion is not wilted after grafting, should increase illumination as far as possible；1-3d after grafting, daytime is covered when illumination is stronger
Lid sunshade net shading, early morning is appropriate at dusk light-exposed, and the time wants short；Gradually extend light application time after 3d, is not required to shade again after 1 week；
When Winter-Spring nursery, such as low temperature sleet or successive cloudy days gas, appropriate light filling is answered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910032154.1A CN109673290B (en) | 2019-01-14 | 2019-01-14 | Watermelon top-inserting grafting method for reducing rootstock sprouting regeneration |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910032154.1A CN109673290B (en) | 2019-01-14 | 2019-01-14 | Watermelon top-inserting grafting method for reducing rootstock sprouting regeneration |
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| CN109673290A true CN109673290A (en) | 2019-04-26 |
| CN109673290B CN109673290B (en) | 2021-02-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113424713A (en) * | 2021-07-27 | 2021-09-24 | 南京市蔬菜科学研究所 | Method for reducing lateral bud germination rate of grafted seedling stock |
| CN115643925A (en) * | 2022-09-07 | 2023-01-31 | 南京市蔬菜科学研究所(南京市花卉科学研究所) | Application of glucose or sucrose in inhibiting growth of terminal bud of watermelon grafting stock |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113424713A (en) * | 2021-07-27 | 2021-09-24 | 南京市蔬菜科学研究所 | Method for reducing lateral bud germination rate of grafted seedling stock |
| CN115643925A (en) * | 2022-09-07 | 2023-01-31 | 南京市蔬菜科学研究所(南京市花卉科学研究所) | Application of glucose or sucrose in inhibiting growth of terminal bud of watermelon grafting stock |
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| Publication number | Publication date |
|---|---|
| CN109673290B (en) | 2021-02-26 |
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