CN105145305B - A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree - Google Patents
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree Download PDFInfo
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- CN105145305B CN105145305B CN201510586045.6A CN201510586045A CN105145305B CN 105145305 B CN105145305 B CN 105145305B CN 201510586045 A CN201510586045 A CN 201510586045A CN 105145305 B CN105145305 B CN 105145305B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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Abstract
The present invention relates to a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, belong to conifer vegetative propagation technique field.The present invention is creatively proposed with obvious 8~10 years raw big trees of wet-land pine tree of growth vigor as maternal plant, in the case where keeping its normal growth, only takes a small amount of side shoot childrenization to handle and be fast and efficiently multiplied into clone using needle-leaved bundle.The inventive method is to break the resting state of needle-leaved bundle by water planting mode of leaving one's post, and necessary Nutrients uptake cell differentiation is provided gradually forms sprouting and growing for callus, root restriction, adventitious root and bud, then sprout form and the completely the same aciculiform acrospire of seedling, new nursery stock is developed into, therefore the inventive method is not restricted by wet-land pine tree mother tree age.In addition, the present invention only needs to carry out excellent maternal plant the " weakness " trimming of a small amount of side shoot, it is ensured that the integrality of maternal plant and the stability of genetic test system, it is easy to carry out large-scale promotion application in production.
Description
Technical field
The present invention relates to conifer vegetative propagation technique field, specifically, the present invention relates to a kind of big tree needle of wet-land pine tree
The water planting method for culturing seedlings of beam.
Background technology
Wet-land pine tree introduces a fine variety history existing more than 60 years of China, and with stronger adaptability, diseases and insect pests resistance is stronger, raw
Length is vigorous, and its trunk is logical straight satisfactory, and Gum yield is high and best in quality, is good papermaking and rosin raw material forest, has driven wood pulp
Papermaking, timber processing and the serial Product processing industrialization of rosin are developed rapidly, with having evolved into south China low mountains and hills
Area's Major Tree Species Planted and important cycle industrial material forest seeds.Although most of coniferous species vegetative propagation is difficult, with nothing
The constantly improve of sexual reproduction technology, vegetative propagation is widely used in the stock breeding of coniferous species more and more.By asexual
Breeding, can choose the good select tree of character from existing germ plasm resource or kind is bred and utilization and extention, both overcome
Seminal propagation nursery stock goes out the shortcoming of garden time length, and the merit of parent can be kept again.With growing directly from seeds or breeding garden conventional breeding journey
Sequence is compared, and its breeding improvement cycle shortens for 1/2~1/3 time than breeding of growing directly from seeds, and can be in the case of the poor identical of selection
Maximum genetic gain, and clone stands forest form is neat are obtained, is easy to intensive farming and processing and utilization, effectively improves forest land
Utilization ratio and the output value.
Wet-land pine tree is studied and is mainly epicormic branch cutting using most conventional nutrient propagation methods at present, passes through construction
Cutting orchard eliminates mother tree age effect through continuous childrenization processing, gathers semi-lignified fringe bar cuttage root-taking under certain environment control
After cultivate into clone.（Torch pine, wet-land pine tree and masson pine Building Techniques of Cuttings Orchard, to hold, Fujian rice wine, 2001,
21(2)；Torch pine, wet-land pine tree and Cuttage Seedling-Raising of Masson Pine technology, to hold, Fujian rice wine, 2001.21 (3)；Wet-land pine tree
The research of cutting orchard and Technique on Cuttage Propagation, Pan Jian, Chen Jiashou, Anhui forestry science and technology, 1997（1）.）.In addition, needle-leaved bundle is
Extremely short the stem in a dormant state for having a needle, needle-leaved bundle water planting nursery is also ripe wet-land pine tree vegetative propagation technique
One of, the technology has ten in terms of construction wet-land pine tree Seed-breeding techniques system, the Developing Clonal Forestry development of propulsion wet-land pine tree
Divide important meaning.（Pine needle Shu Yumiao, Zhou Xintie, Yang Chenggui, China Forestry Publishing House, 1988；Needle of Slash Pine Shu Miao
Clonal planting is studied, Zhou Xintie etc., Hubei forestry science and technology, 1990, (3):14-16；The preliminary of Needle of Slash Pine Shu Shuipei is ground
Study carefully, Li Tingting, Xu Ye continent etc., Hubei forestry science and technology, 2010.）But it is female that above two asexual reproduction method is only applicable to young age
Strain, give birth to the then spray or needle-leaved bundle gathered from the big tree of pine tree of 8 years raw above is difficult to breed seedling by the above method, i.e.,
Make its nursery stock also growth retardation of taking root on a small quantity, and its survival rate is also very low.As can be seen here, routine side disclosed in prior art
Method is not suitable for the big tree needle-leaved bundle of non-young age maternal plant wet-land pine tree and carries out vegetative propagation, and prior art also rarely has particularly for non-children
Age conifer maternal plant carries out the report in terms of quick, efficient, extensive vegetative propagation technique.
In summary, how preferably to overcome the age effect in restriction conifer vegetative propagation technique and position effect, dash forward
It is marred by a scar, etc. pass technological difficulties, is current researcher technical problem urgently to be resolved hurrily.
The content of the invention
The deficiency that the problem of present invention is directed to pointed in background technology and prior art are present, it is an object of the invention to
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree is provided, this method is creatively proposed with growth vigor obvious 8
The raw big tree of wet-land pine tree is maternal plant within~10 years, in the case where keeping its normal growth, takes a small amount of side shoot childrenization to handle and profit
Clone is fast and efficiently multiplied into needle-leaved bundle, hence it is evident that shorten breeding cycle, can carry out amount reproduction, advance significantly
Improved variety process, breaches the age that traditional conifer clonal reproduction method is only applicable to the needle-leaved bundle nursery of 1~2 year raw nursery stock
Effect defect；And this method only needs to carry out excellent maternal plant the " weakness " trimming of a small amount of side shoot, it is ensured that the integrality and something lost of maternal plant
Pass the stability of measurement system, it is easy to carry out large-scale promotion application in production.
In order to realize the above-mentioned purpose of the present invention, inventor passes through substantial amounts of experimental study, have developed a kind of wet-land pine tree
The water planting method for culturing seedlings of big tree needle-leaved bundle, the technical scheme of methods described is as follows：
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, methods described is comprised the following steps：
（1）The larger fine individual plant of growth selection amount, is entered by fast-growing, high-yield woods technical regulation in 8~10 years raw standing forests
Row tending management, rationally control density keep sufficient tree crown growing space, by loosening the soil and apply fertilizer etc., measure promotes forest strong
Strong growth；
（2）2~5 tree crown middle and lower parts of selection are healthy and strong, the preferable primary lateral branches of illumination condition, right before the flowing of early spring resin
It carries out trimming rush and sprouted；
（3）Erase in time for 4~May small and weak or excessive rudiment, make 2~3 healthy and strong rudiment fast-growths into young sprout coppice shoot；
（4）Work as step（3）The coppice shoot grows into before semi-lignified, its terminal bud are not sprouted and pruned away for the second time
Top, retains 3~5cm of length, after small and weak or excessive rudiment of being erased after rudiment, and every retains 1~2 healthy and strong rudiment；
（5）Mid or late October erases young sprout terminal bud, interrupts fringe bar top elongation growth, promotes needle-leaved bundle nutrient reserve simultaneously to tie up
Hold its youngization state；
（6）Collection handles the needle-leaved bundle on coppice shoot by childrenization after mid or late December maternal plant stops growing；
（7）Selection room ventilation is ventilative, the place of without sunlight direct projection, builds sand bed, uses mass concentration for 0.2~0.5%
KMnO4Solution is by step（6）The needle-leaved bundle collected carries out disinfection, and is then drenched with water purification, and needle-leaved bundle is upright
During embedment is husky, the needle-leaved bundle length of embedment is the 1/2 of needle-leaved bundle length, pours once permeable after having buried immediately, and cover film
Moisturizing, it is 5~8 DEG C to keep indoor temperature, keeps sand bed moistening, and the sand storage time is 25~35 days；
（8）Water planting frame is modulated into tissue culture frame, two layers above and below water planting frame point, from translucency is good, high-temp resisting high-humidity resisting, easily
The vial of cleaning is as water planting bottle, and drying is standby after being rinsed well after the vial is carried out disinfection before water planting with clear water
With；
（9）Weigh a certain amount of boric acid, vitamin B1, ammonium nitrate be dissolved in water, be configured to nutrient solution, stand-by, the culture
The concentration of liquid mesoboric acid is 75mg/L, vitamin B1Concentration be 20mg/L, the concentration of ammonium nitrate is 20mg/L, the nutrient solution
PH value be 6.0~6.5；
（10）It is general to start water planting nursery in mid-February, needle-leaved bundle is first taken out from sand bed and most fine sand is washed, leaf color is selected
Normally, base portion parcel is loose or slightly has the healthy and strong needle-leaved bundle expanded, cuts off base portion with blade, makes otch smooth, using carbendazim
Taken out after solution or Bravo solution immersion needle-leaved bundle base portion 30min, needle-leaved bundle base portion is managed, the life prepared in advance is put into
In root element solution, soak time is 12~16h, and needle-leaved bundle is taken out after the completion of processing and residual liquor is cleaned up with clear water；
（11）By step（9）The nutrient solution prepared adds step（8）In described water planting bottle, liquid level is controlled
Every needle-leaved bundle base portion is set all to be immersed in nutrient solution, often in insertion bottle for 1.0~1.5cm, then after needle-leaved bundle is dismissed by bundle
30~50, bottle, put it is uniform, then by the water planting bottle for installing needle-leaved bundle and nutrient solution it is neat, be equably placed in water planting frame
On, it is subsequently placed in plastic, thermal-insulation canopy or glasshouse, is not contacted with each other as degree with the blade tip of needle-leaved bundle；
（12）Maximum temperature in auxiliary heating, control roof insulating or glasshouse is carried out with electric heater and is no more than 35
DEG C, minimum temperature is not less than 12 DEG C, and it is 60~80% to control relative air humidity in roof insulating or glasshouse, such as strong day
According to need to carry out appropriate shading, night, which is used, keeps the depth of nutrient solution in water planting bottle not low during fluorescent lamp supplementary light, water planting
In 1cm, if not enough, step need to be added in time（9）Described nutrient solution；A foliar fertilizer was sprayed every 7 days, the fertilizer is by step
Suddenly（9）The culture formula of liquid is prepared, and is once cleaned and changes nutrient solution within every 7~10 days；
（13）30~40 days needle-leaved bundles of water planting are taken root, and start to transplant when root length reaches 1~2cm：First in seedling before transplanting
The deep ditches of 2~3cm are excavated by 18~20cm line-spacing on bed surface, the thick pure thin river sands of 2cm are spread in ditch, are transplanted first 1~2 day
Drench river sand, need to be carried out during transplanting under moisturizing dark conditions, an aperture is opened on fine sand with scuppit or waddy, rooted seedling is put
Enter in hole, root system is unfolded and is combined closely with sandy soil, the depth of planting is that seedling root neck is slightly below native face, and spacing in the rows is 3~5cm, is planted
Pour permeable after complete immediately, transplanted after being truncated when root length is more than 5 cm, put arch bamboo chip on seedbed surface after transplanting, cover film thermal insulating
Moisturizing；
（14）Strengthen management, the humidity of running check transplanting medium, after removing film after Survival rate of nursery stock, is transplanted to seedbed
Needle-leaved bundle start within 10 days or so to grow new root, original water root by white be transformed into brown, lignifying after be gradually transformed into
Normal root system；
（15）Two months or so needle-leaved bundle germinating to seedling, after bud is unearthed, carries out weeding by field seedling raising method, apply fertilizer, pour
The seedling managements such as water, the prevention and control of plant diseases, pest control.
Further, above-mentioned technical proposal step（2）Described in pruning method be：By primary lateral branches and two grades of sturdy side shoots
Leading shoot was blocked at spring upper one year life branch, and other side shoots are wiped out at the blight wood of autumn growth upper one year, while wiping out the weak branch of disease branch.
Further, above-mentioned technical proposal step（7）Described in the sand bed built made with brick or plank, the sand
80~100cm of bedside, deep 20~30cm, the needle-leaved bundle neat bury in sand bed put into some rows, and with bundled phase in a line
Company, which buries, puts, and it is 8~10cm that the needle-leaved bundle, which buries the line-spacing put,.
Further, step in above-mentioned technical proposal（8）The water planting frame bottom is highly 50cm, between two layers at intervals of
80~100cm, the vial is cylindrical glass bottle, and the internal diameter of the vial is 6~8cm, interior a height of 8~10cm.
It is further preferred that silica-gel electrothermic plate is covered with described in above-mentioned technical proposal on water planting frame.
Further, step in above-mentioned technical proposal（10）Described in needle-leaved bundle root in carbendazim or Bravo solution
The soaking depth in year is 1~2cm；Soaking depth of the needle-leaved bundle in root element solution is 1~2cm, and the carbendazim is molten
What liquid was selected is 50% carbendazim, 500 times of liquid, and what the Bravo solution was selected is 70% Bravo, 800 times of liquid, the root element
Solution is NAA, IBA that concentration is 50mg/L or 911 root element solution.
Further, above-mentioned technical proposal step（12）Described in control day temperature be 25~28 DEG C, the night temperature
Spend for 15~18 DEG C.
Further, above-mentioned technical proposal step（11）Described in the selection of plastic, thermal-insulation canopy having a good transport service, illumination it is sufficient,
Ventilated, flatly building close to water source, the height of the roof insulating is that 2~3m, the length of side are 10~25m, ceiling
For arch or the Simple double-layer vinyl house on inclined-plane, heating, humidification, illuminator or facility are provided with the roof insulating.
Further, above-mentioned technical proposal step（12）Described in the shading time between the ︰ 00 of 11 ︰ 00~15, open hide
Shade net carries out appropriate shading, the night supplementary light time be 18 ︰ 00 to the ︰ 00 of next day 7, use intensity of illumination for 2000lx's
Fluorescent lamp supplementary light.
Further, above-mentioned technical proposal step（12）Described in keep medium depth be 1.0~1.5cm.
Compared with prior art, the present invention has following beneficial effect：
（1）The plant nutrient tissue that the present invention is used to breed nursery stock is needle-leaved bundle, hence it is evident that be different from prior art use
Side shoot or rudiment bar, needle-leaved bundle are extremely short the stems for having a needle, the shaping age of pine tree sprouting are typically grown on, in its shape
Also break up budding simultaneously during, the breeding coefficient of defect individual early stage can not only be improved with needle-leaved bundle breeding nursery stock, and
The inclined hat or base portion germinating phenomenon of tloig-cutlage seedling can be overcome, be that the production of breeding clone nursery stock technology illustrates prospect；
（2）The present invention is different from traditional cuttage and seedling culture method, and the present invention breaks stopping for needle-leaved bundle by water planting mode of leaving one's post
Dormancy state, and necessary Nutrients uptake cell differentiation is provided gradually forms sprouting for callus, root restriction, adventitious root and bud
With growth, then sprout form and the completely the same aciculiform acrospire of seedling, develop into new nursery stock；And the present invention can be protected
The youngization state of the big tree needle-leaved bundle of wet-land pine tree is held, therefore the inventive method is not restricted by wet-land pine tree mother tree age, can be applied to 8
The big tree of~10 years raw wet-land pine trees, preferably overcomes the age effect of the maternal plant in prior art restriction conifer vegetative propagation technique
Should and position effect；
（3）The " weakness " that the present invention only carries out a small amount of side shoot to excellent maternal plant is trimmed, the influence to maternal plant normal growth and development
It is little, the integrality of pilot forest and the systematicness of genetic test were both ensure that, to carry out to it longer-term, more scientific system
Genetic test, can keep original material to carry out more massive breeding and promote again；
（4）The big tree needle-leaved bundle rooting rate of wet-land pine tree that the inventive method is obtained is high, and with higher breeding coefficient：By 1
Strain maternal plant handles 3 side shoots, and every side shoot retains 20 coppice shoots, and every coppice shoot can gather 40 qualified needle-leaved bundles, with rooting rate
Counted with planting percent 70%, 1000 plants of clone nursery stocks can be produced year, fast breeding and can promoted the use of fine strains；
（5）The inventive method effectively shortens breeding cycle, with grow directly from seeds or breeding garden conventional breeding programmes compared with, the application can
Shorten the time of breeding cycle 1/2~1/3, the genetic gain of maximum can be obtained in the case of the poor identical of selection, and it is asexual
It is standing forest forest form is neat, is easy to intensive farming and processing and utilization, effectively improves forest land utilization ratio and the output value；
（6）Compared with the technologies such as tissue culture, body cell generation, the inventive method operation is relatively simple, and cost is relatively low, it is easy to
Popularization and application in production.
Embodiment
Technical scheme is described in detail below by specific embodiment.
Embodiment 1
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, methods described comprises the following steps：
（1）Fine individual plant in 8 years raw clonal test Lin Zhongxuans of wet-land pine tree select 2 choiceness 82-15,86-8
Each 1 plant, listing mark；（2）Before resin flowing at the beginning of 3 months, 2~3 tree crown middle and lower part growths are selected in every plant of fine individual plant
Healthy and strong, the preferable side shoot of illumination condition is trimmed：First by other sides in addition to primary lateral branches or two grades of sturdy side shoot leading shoots
Branch is amputated at the blight wood of autumn growth upper one year, small and weak or undergrowth side shoot is then all amputated from raw base portion, then in master
Blocked on the tip at 1 year spring life branch, only retain 3~5cm length have the leading shoot base portion of needle-leaved bundle, it is ensured that the development of this section is good for
It is strong；（3）Rudiment at the top of the disconnected branch of observation in 4~May, small and weak or excessive rudiment of erasing in time is filled with ensureing that 2~3 healthy and strong rudiments have
The nutrition of foot and growing space, promote its sturdy, fast-growth into young sprout；（4）When coppice shoot grows into semi-lignified, its terminal bud not
Top is pruned away before sprouting for the second time, retains 3~5cm of length, after small and weak or excessive rudiment of being erased after rudiment, every reservation
1~2 healthy and strong rudiment；（5）Mid or late October young sprout terminal bud of erasing interrupts fringe bar top elongation growth, promotes needle-leaved bundle nutrition storage
It is standby and maintain its youngization state；（6）Collection handles the needle-leaved bundle on coppice shoot by childrenization after mid or late December maternal plant stops growing：
Fringe bar first half robust growth, development health, the needle-leaved bundle of no disease and pests harm are selected, needle-leaved bundle base portion is pinched with thumb and forefinger,
Needle-leaved bundle is removed together with fringe bar epidermis, it is to avoid needle-leaved bundle scatters, base portion is arranged neat, elastic appropriateness ties up bunchy；
（7）Selection room ventilation is ventilative, the place of without sunlight direct projection, and growth 5m, wide 80cm, deep 20cm sand bed, from punching are of brick
Wash clean, the river sand of medium-coarse as matrix, the needle-leaved bundle used before sand storage mass concentration for 0.5% KMnO4Solution
Water purification is drenched after sterilization, is then uprightly put into needle-leaved bundle in wet sand, and length of embedment is the 1/2 of needle-leaved bundle length, vertical after having buried
Pour once permeable, and cover film moisturizing, it is 6 DEG C to keep indoor temperature, keep sand bed moistening, needle-leaved bundle sand bed is refrigerated 28
My god；（8）Selection has a good transport service, illumination is sufficient, it is ventilated, close to water source flatly, it is that 2.5m, the length of side are to build height
15m, ceiling are the Simple double-layer vinyl house on arch or inclined-plane, and install heating, humidification, illuminator in warm canopy or set
Apply, to regulate and control warm canopy humiture and illumination condition；（9）It is modulated into tissue culture frame above and below water planting frame, water planting frame point two layers, bottom is high
Spend for 50cm, at intervals of 80cm between two layers, from translucency is good, high-temp resisting high-humidity resisting, easily cleaning, internal diameter is 8cm, interior a height of
8cm vial is as water planting bottle, with the KMnO that mass concentration is 0.5% before water planting4Solution immersion 30min sterilizations, clear water is rinsed
Drying for standby after clean；（10）75mg boric acid, 20mg vitamin Bs are weighed respectively1, 20mg ammonium nitrate be dissolved in 1L deionized waters,
Nutrient solution is configured to after mixing, stand-by, the concentration of the nutrient solution mesoboric acid is 75mg/L, vitamin B1Concentration be 20mg/
L, the concentration of ammonium nitrate is 20mg/L, and the pH value of the nutrient solution is 6.0~6.5；（11）Start water planting within 10~12 days 2 months to educate
Seedling, drains away the water after first sand storage needle-leaved bundle is cleaned with clear water, selection leaf color is normal, base portion parcel is loose or slightly expand it is strong
Strong needle-leaved bundle, continues to use the base portion that sharp blade vertically cuts off needle-leaved bundle, it is ensured that cut under needle-leaved bundle base portion needle attachment region
Mouth is smooth, then steeps needle-leaved bundle base portion 30min with 50% carbendazim, 500 times of immersions, and needle-leaved bundle root soaking depth is 1~2cm, is disappeared
Needle-leaved bundle base portion is managed after poison, is put into the 50mg/L NAA root element solution prepared in advance and soaks in batch, soaking depth
Needle-leaved bundle is taken out for from the upward 1~2cm in needle-leaved bundle root, soak time is 14h, after the completion of processing and with clear water by residual liquor
Clean up；（12）By step（10）The nutrient solution prepared adds step（9）In described water planting bottle, control liquid level is high
Spend for 1.0~1.5cm, then every needle-leaved bundle base portion is all immersed in culture in insertion water planting bottle after needle-leaved bundle is dismissed by bundle
In liquid, 30~50 every bottle, put uniformly, then neatly, be equably placed in the water planting bottle for installing needle-leaved bundle and nutrient solution
On water planting frame, do not contacted with each other as degree, water planting is placed in warm canopy with the blade tip of needle-leaved bundle then；（13）Entered with electric heater
Row auxiliary heating, it is 25~28 DEG C to control day temperature in warm canopy room, and nocturnal temperature is 15~18 DEG C, and humidity is 60~80%；Such as
Meet strong sunlight selection and appropriate shading is carried out between the ︰ 00 of 11 ︰ 00~15, night uses fluorescent lamp supplementary light, and supplementary light is strong
Spend for 2000lx, the supplementary light time is to keep the depth of nutrient solution in water planting bottle not during 18 ︰ 00 to the ︰ 00 of next day 7, water planting
Less than 1cm, if not enough, step need to be added in time（10）Described nutrient solution；A foliar fertilizer, the fertilizer were sprayed every 7 days
By step（10）The culture formula of liquid is prepared, and is once cleaned and changes nutrient solution within every 7~10 days；Fair weather Indoor Air
Temperature rise to or during more than 35 DEG C, open that to can adjust door and window in warm canopy aeration-cooling in time, and be subject to appropriate shading and spraying, keep
Sanitation and hygiene in warm canopy, remove the source of infection in time；（14）Needle-leaved bundle water planting starts to produce callus, 20 days or so for 15 days or so
Root shape projection is initially formed, rooting rate is up to 78.6% at 30 days, and average every needle-leaved bundle is taken root 3.2；Before and after Clear and Bright, work as root
Length is transplanted when reaching 1cm~2cm：First 2cm~3cm depths are excavated during transplanting by 18cm~20cm line-spacing on seedbed
Ditch, the thick pure thin river sands of 2cm are spread in ditch, preceding 1~2 day river sand of drenching is transplanted, the moisturizing screening of rooted seedling is noted during transplanting
Light, opens an aperture with scuppit or waddy on fine sand, rooted seedling is put into hole, it is ensured that root system is unfolded and closely tied with sandy soil
Close, the depth of planting is that seedling root neck is slightly below native face, and spacing in the rows 3cm~5cm pours permeable, truncated when root length is more than 5cm immediately after having planted
After transplant, arch bamboo chip is put after transplanting on seedbed, film thermal insulating moisturizing is covered；（15）It is vaporific daily in 20 days after the transplanting of crop field
Water 1~2 time, side is irrigated when damage caused by a drought is heavier, keep the water surface to be less than in 3~5cm of bed surface, 40 days after the transfer, enter weekly
Foliage dressing of row, loosens the soil, keeps Seedling rhizosphere topsoil loose in time after raining or repeatedly water every time when bud being unearthed,
In order to avoid the hardened influence rooted seedling of matrix is survived and germinateed, too deep soil layer is flowed or blow over and cover completely in removing, and righting lodging, crooked or needle are attached
In the nursery stock of bed surface, the needle-leaved bundle for being transplanted to seedbed starts to grow new root for 10 days or so, and water root originally is transformed into by white
Gradually it is transformed into normal root system after brown, lignifying；（16）Two months or so needle-leaved bundles can germinating to seedling, planting percent reaches
74.3%, after bud is unearthed, by field seedling raising method carry out weeding, apply fertilizer, water, the seedling management such as the prevention and control of plant diseases, pest control.
Embodiment 2
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, methods described comprises the following steps：
（1）Determined by the increment of 9 years raw wet-land pine tree High yielding family trial woodss, select 3 superior families G354,
G367, G73, select each 1 plant of fine individual plant, listing mark respectively；（2）Before the flowing of resin by the end of February, every plant of fine individual plant selection
2~3 tree crown middle and lower part robust growths, the preferable side shoots of illumination condition are trimmed, by primary lateral branches and two grades of sturdy side shoots
Leading shoot is blocked at spring the previous year life branch, and other side shoots are wiped out at the blight wood of autumn growth upper one year, while wiping out the weak branch of disease branch, only
Retain 3~5cm length have the leading shoot base portion of needle-leaved bundle, and ensure this section development stalwartness；（3）4~May erases in time
Small and weak or excessive rudiment, it is ensured that 2~3 healthy and strong rudiment fast-growths are into young sprout；（4）When coppice shoot grows into semi-lignified, Qi Ding
Bud is pruned away top for the second time before not sprouting, retain 3~5cm of length, after the small and weak or excessive rudiment, every of being erased after rudiment
Retain 1~2 healthy and strong rudiment；（5）Mid or late October erases young sprout terminal bud, interrupts fringe bar top elongation growth, promotes needle-leaved bundle
Nutrient reserve simultaneously maintains its youngization state；（6）Collection is by childrenization processing coppice shoot after mid or late December maternal plant stops growing
Needle-leaved bundle, selection fringe bar first half robust growth, development health, the needle-leaved bundle of no disease and pests harm, needle is pinched with thumb and forefinger
Beam base portion, removes needle-leaved bundle, it is to avoid needle-leaved bundle scatters together with fringe bar epidermis, and base portion is arranged into neat, elastic appropriateness bundle
It is bundled into beam；（7）Selection room ventilation is ventilative, the place of without sunlight direct projection, and growth 6m, wide 100cm, deep 30cm sand are of plank
Bed, from rinsing well, the river sand of medium-coarse as matrix, the needle-leaved bundle is 0.5% with mass concentration before sand storage
KMnO4Water purification is drenched after solution carries out disinfection, and is then uprightly put into needle-leaved bundle in wet sand, and the needle-leaved bundle is neat in sand bed
Bury and put into some rows, and put with bundled connected bury in a line, it is 10cm that the needle-leaved bundle, which buries the line-spacing put, and length of embedment is needle
The 1/2 of Shu Changdu, pours once permeable immediately after having buried, and covers film moisturizing, and it is 8 DEG C to keep indoor temperature, keeps sand bed wet
Profit, needle-leaved bundle sand bed is refrigerated 25 days；（8）It is modulated into tissue culture frame above and below water planting frame, water planting frame point two layers, bottom is highly
50cm, at intervals of 100cm between two layers, from translucency is good, high-temp resisting high-humidity resisting, easily cleaning, internal diameter is 6cm, interior a height of 8cm
Vial as water planting bottle, with the KMnO that mass concentration is 0.2% before water planting4Solution immersion 30min sterilizations, clear water rinses dry
Drying for standby after net；（9）75mg boric acid, 20mg vitamin Bs are weighed respectively1, 20mg ammonium nitrate be dissolved in 1L deionized waters, mix
After be configured to nutrient solution, stand-by, the concentration of the nutrient solution mesoboric acid is 75mg/L, vitamin B1Concentration be 20mg/L, nitre
The concentration of sour ammonium is 20mg/L, and the pH value of the nutrient solution is 6.0~6.5；（10）Start water planting nursery, water planting within 4~6 days 2 months
Room uses glasshouse, is drained away the water after first sand storage needle-leaved bundle is cleaned with clear water, selection leaf color is normal, base portion parcel is loose or
The healthy and strong needle-leaved bundle slightly expanded, under needle-leaved bundle base portion needle attachment region continuing to use sharp blade vertically cuts off needle-leaved bundle
Base portion, it is ensured that otch is smooth, then steep needle-leaved bundle base portion 30min, needle-leaved bundle root soaking depth with 70% Bravo, 800 times of immersions
For 1~2cm, needle-leaved bundle base portion is managed after sterilization, is put into the root element solution of 50mg/L 911 prepared in advance and soaks in batch
Bubble, soaking depth is that soak time is 16h from 1 upward~2cm of needle-leaved bundle root, and needle-leaved bundle is taken out after the completion of processing and is used in combination
Clear water cleans up residual liquor；（11）By step（9）The nutrient solution prepared adds step（8）Described water planting bottle
In, it is 1.0~1.5cm to control liquid level, then needle-leaved bundle is made into every needle-leaved bundle base portion by being inserted after bundle dismission in water planting bottle
All it is immersed in nutrient solution, 30~50 every bottle, puts uniformly, do not contacted with each other for degree, then by water with the blade tip of needle-leaved bundle
Training bottle is uniformly placed on the water planting frame for being covered with silica-gel electrothermic plate；（12）Computer heating control nutrient solution is aided in by using silica-gel electrothermic plate
Temperature, control nutrient solution temperature range be 18 DEG C~25 DEG C, humidity is by phjytotron atomizer or sprayer unit program
Control, setting air relative humidity is 60~80%, and night uses fluorescent lamp supplementary light, in case of strong sunlight is fitted at noon
The depth of nutrient solution is not less than 1cm in being kept during degree shading, water planting, checks whether needle-leaved bundle base portion is immersed in sooner or later daily
In nutrient solution, nutrient solution is added in time, prevents dehydration；Once cleaned and change nutrient solution within every 7~10 days, by pin during replacing
Ye Shu takes out from container, and clear water changes fresh medium after cleaning needle-leaved bundle base portion and vial；（13）During water planting 12 days
Start to produce callus, root shape projection is initially formed at 18 days, rooting rate is up to 74.2% at 30 days, average every needle-leaved bundle life
Root 2.8, by the end of March at the beginning of 4 months, is transplanted when root length reaches 1~2cm：18cm is first pressed during transplanting on the face of seedbed
~20cm line-spacing excavates the deep ditches of 2cm~3cm, and the thick pure thin river sands of 2cm are spread in ditch, preceding 1~2 day river of drenching is transplanted
Sand, notes the moisturizing shading of rooted seedling, an aperture is opened on fine sand with scuppit or waddy, rooted seedling is put into hole during transplanting,
Ensure that root system is unfolded and combined closely with sandy soil, the depth of planting is that seedling root neck is slightly below native face, and spacing in the rows is 3cm~5cm, after having planted
Pour immediately permeable；Transplanted after being truncated when root length is more than 5 cm, put arch bamboo chip after transplanting on seedbed, cover film thermal insulating moisturizing；
（14）Strengthen management, the humidity of running check transplanting medium is sprayed when watering using watering can, in case the hardened influence of matrix is taken root
Seedling is survived and germinateed, after removing film after Survival rate of nursery stock, and the needle-leaved bundle for being transplanted to seedbed starts to grow new root for 10 days or so, originally
Water root be transformed into by white after brown, lignifying and be gradually transformed into normal root system；（15）Two months or so needle-leaved bundles
Germinating to seedling, planting percent is up to 71.7%, after bud is unearthed, by field seedling raising method carry out weeding, apply fertilizer, water, the prevention and control of plant diseases, pest control etc.
Seedling management.
Embodiment 3
A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, methods described comprises the following steps：
（1）Provenance Test for Exotic Pinus elliottii Lin Zhongxuan, which is originated in, in the 10 Nian Sheng U.S. selects 3 plants of individual plants；（2）In resin flowing mid-February
Before, select 2~3 tree crown middle and lower part robust growths, the preferable side shoots of illumination condition to be trimmed in every plant of fine individual plant, will
Primary lateral branches and two grades of sturdy side shoot leading shoots are blocked at spring the previous year life branch, and other side shoots are cut at the blight wood of autumn growth upper one year
Remove, only retain 3~5cm length have the leading shoot base portion of needle-leaved bundle, while wiping out the weak branch of disease branch；（3）4~May erases in time
Small and weak or excessive rudiment, it is ensured that 2~3 healthy and strong rudiment fast-growths are into young sprout；（4）When coppice shoot grows into semi-lignified, Qi Ding
Bud is pruned away top for the second time before not sprouting, retain 3~5cm of length, after the small and weak or excessive rudiment, every of being erased after rudiment
Retain 1~2 healthy and strong rudiment；（5）Mid or late October erases young sprout terminal bud, interrupts fringe bar top elongation growth, promotes needle-leaved bundle
Nutrient reserve simultaneously maintains its youngization state；（6）Collection is by childrenization processing coppice shoot after mid or late December maternal plant stops growing
Needle-leaved bundle, selection fringe bar first half robust growth, development health, the needle-leaved bundle of no disease and pests harm, needle is pinched with thumb and forefinger
Beam base portion, removes needle-leaved bundle, it is to avoid needle-leaved bundle scatters together with fringe bar epidermis, and base portion is arranged into neat, elastic appropriateness bundle
It is bundled into beam；（7）Selection room ventilation is ventilative, the place of without sunlight direct projection, and growth 8m, wide 100cm, deep 25cm sand are of plank
Bed, from rinsing well, the river sand of medium-coarse as matrix, the needle-leaved bundle is 0.2% with mass concentration before sand storage
KMnO4Water purification is drenched after sterilization, is then uprightly put into needle-leaved bundle in wet sand, if the needle-leaved bundle neat bury in sand bed is put into
Dry row, and put with bundled connected bury in a line, it is 8cm that the needle-leaved bundle, which buries the line-spacing put, and length of embedment is needle-leaved bundle length
1/2, pour once permeable after having buried immediately, and film moisturizing is covered, it is 5 DEG C to keep indoor temperature, sand bed moistening is kept, by needle
Beam sand bed is refrigerated 35 days；（8）Be modulated into water planting frame with tissue culture frame, two layers above and below water planting frame point, bottom highly is 50cm, two layers it
Between at intervals of 80cm, from translucency is good, high-temp resisting high-humidity resisting, easily cleaning, internal diameter is 8cm, and interior a height of 10cm vial is made
For water planting bottle, with the KMnO that mass concentration is 0.5% before water planting4Solution immersion 30min is carried out disinfection, and clear water is done after rinsing well
It is dry standby；（9）Weigh 75mg boric acid, 20mg vitamin Bs1, 20mg ammonium nitrate be dissolved in 1L deionized waters, be configured to after mixing training
Nutrient solution, stand-by, the concentration of the nutrient solution mesoboric acid is 75mg/L, vitamin B1Concentration be 20mg/L, the concentration of ammonium nitrate
For 20mg/L, the pH value of the nutrient solution is 6.0~6.5；（10）Start water planting within 14 days 2 months, water planting room uses glasshouse；First
Drained away the water after sand storage needle-leaved bundle is cleaned with clear water, selection leaf color is normal, base portion parcel is loose or healthy and strong pin that is slightly expanding
Ye Shu, the base portion that sharp blade vertically cuts off needle-leaved bundle is continued to use under needle-leaved bundle base portion needle attachment region, it is ensured that otch is put down
It is whole, then needle-leaved bundle base portion 30min is steeped with 50% carbendazim, 500 times of immersions, needle-leaved bundle root soaking depth is 1~2cm, after sterilization
Needle-leaved bundle base portion is managed, be put into batch in the root element solution of 50mg/L 911 prepared in advance soak, soaking depth be from
1 upward~2cm of needle-leaved bundle root, soak time is 18h, needle-leaved bundle is taken out after the completion of processing and with clear water that residual liquor is clear
Wash clean；（11）By step（9）The nutrient solution prepared adds step（8）In described water planting bottle, liquid level is controlled
Every needle-leaved bundle base portion is set all to be immersed in nutrient solution in insertion water planting bottle for 1.0~1.5cm, then after needle-leaved bundle is dismissed by bundle
In, 30~50 every bottle, put uniformly, do not contacted with each other as degree, be then uniformly placed in water planting bottle with the blade tip of needle-leaved bundle
It is covered with the water planting frame of silica-gel electrothermic plate；（12）The temperature of computer heating control nutrient solution, control training are aided in by using silica-gel electrothermic plate
The temperature range of nutrient solution is 18 DEG C~25 DEG C, and humidity is by phjytotron atomizer or sprayer unit programme-control, setting air
Relative humidity 60~80%, night uses fluorescent lamp supplementary light, in case of strong sunlight carries out appropriate shading, water planting process at noon
The depth of nutrient solution is 1~1.5cm in middle holding, checks whether needle-leaved bundle base portion is immersed in nutrient solution sooner or later daily, in time
Nutrient solution is added, dehydration is prevented；Once cleaned and change nutrient solution within every 7~10 days, during replacing by needle-leaved bundle from container
Take out, clear water changes fresh medium after cleaning needle-leaved bundle base portion and vial；（13）Start to produce callus during water planting 13 days
Tissue, starts to take root at 20 days, and rooting rate is up to 68.8% at 30 days, April first arrival April 18, when root length reaches 1~2cm i.e.
Transplanted：The deep ditches of 2cm~3cm first are excavated by 18cm~20cm line-spacing on the face of seedbed during transplanting, 2cm are spread in ditch thick
Pure thin river sand, transplant preceding 1~2 day river sand of drenching, the moisturizing shading of rooted seedling noted during transplanting, with scuppit or waddy thin
An aperture is opened on sand, rooted seedling is put into hole, it is ensured that root system is unfolded and combined closely with sandy soil, the depth of planting is omited for seedling root neck
Less than native face, spacing in the rows 3cm~5cm pours permeable immediately after having planted, and transplants, is put after transplanting on seedbed after being truncated when root length is more than 5cm
Arch bamboo chip, covers film thermal insulating moisturizing；（14）Strengthen management, the humidity of running check transplanting medium uses watering can when watering
Spray, in order to avoid the hardened influence rooted seedling of matrix is survived and germinateed, after removing film after Survival rate of nursery stock, is transplanted to the needle-leaved bundle in seedbed
Start within 10 days or so to grow new root, original water root is transformed into after brown, lignifying by white is gradually transformed into normal root system；
（15）Two months or so needle-leaved bundles can germinating to seedling, planting percent is up to 85.7%.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (9)
1. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree, it is characterised in that：Methods described is comprised the following steps：
（1）The larger fine individual plant of growth selection amount, is comforted by fast-growing, high-yield woods technical regulation in 8~10 years raw standing forests
Management is educated, rationally control density keeps sufficient tree crown growing space, forest robust growth is promoted with measures of fertilizer by loosening the soil；
（2）2~5 tree crown middle and lower parts of selection are healthy and strong, the preferable side shoot of illumination condition, and it is repaiied before the flowing of early spring resin
Rush is cut to sprout；
（3）Erase in time for 4~May small and weak or excessive rudiment, make 2~3 healthy and strong rudiment fast-growths into young sprout coppice shoot；
（4）Work as step（3）The coppice shoot grows into before semi-lignified, its terminal bud are not sprouted and is pruned away top for the second time, protects
3~5cm of length is stayed, after small and weak or excessive rudiment of being erased after rudiment, every retains 1~2 healthy and strong rudiment；
（5）Mid or late October erases young sprout terminal bud, interrupts fringe bar top elongation growth, promotes needle-leaved bundle nutrient reserve simultaneously to maintain it
Youngization state；
（6）Collection handles the needle-leaved bundle on coppice shoot by childrenization after mid or late December maternal plant stops growing；
（7）Selection room ventilation is ventilative, the place of without sunlight direct projection, builds sand bed, uses mass concentration for 0.2~0.5%
KMnO4 solution is by step（6）The needle-leaved bundle collected is carried out disinfection, and is then drenched with water purification, and needle-leaved bundle is uprightly buried
Enter in sand, the needle-leaved bundle length of embedment is the 1/2 of needle-leaved bundle length, pours once permeable after having buried immediately, and cover film guarantor
Wet, it is 5~8 DEG C to keep indoor temperature, keeps sand bed moistening, and the sand storage time is 25~35 days；
（8）Water planting frame is modulated into tissue culture frame, two layers above and below water planting frame point, from translucency is good, high-temp resisting high-humidity resisting, easily cleaning
Vial as water planting bottle, drying for standby after being rinsed well after the vial is carried out disinfection before water planting with clear water；
（9）Weigh a certain amount of boric acid, vitamin B1, ammonium nitrate and be dissolved in water, be configured to nutrient solution, it is stand-by, in the nutrient solution
The concentration of boric acid is 75mg/L, and the concentration of vitamin B1 is 20mg/L, and the concentration of ammonium nitrate is 20mg/L, the pH of the nutrient solution
It is worth for 6.0~6.5；
（10）Start water planting nursery in mid-February, needle-leaved bundle is first taken out from sand bed and most fine sand is washed, selection leaf color is normal, base
Portion's parcel is loose or slightly has the healthy and strong needle-leaved bundle expanded, cuts off base portion with blade, makes otch smooth, using carbendazim solution or hundred
Taken out after bacterium clear solution immersion needle-leaved bundle base portion 30min, needle-leaved bundle base portion is managed, the root element solution prepared in advance is put into
In, soak time is 12~16h, and needle-leaved bundle is taken out after the completion of processing and residual liquor is cleaned up with clear water；
（11）By step（9）The nutrient solution prepared adds step（8）In described water planting bottle, the liquid level is controlled to be
1.0~1.5cm, then every needle-leaved bundle base portion is all immersed in nutrient solution by insertion bottle after bundle dismission needle-leaved bundle, every bottle
30~50, put uniformly, then neatly, be equably placed in the water planting bottle for installing needle-leaved bundle and nutrient solution on water planting frame,
It is subsequently placed in plastic, thermal-insulation canopy or glasshouse, is not contacted with each other as degree with the blade tip of needle-leaved bundle；
（12）Maximum temperature in auxiliary heating, control roof insulating or glasshouse is carried out with electric heater and is no more than 35 DEG C, most
Low temperature is not less than 12 DEG C, and it is 60~80% to control relative air humidity in roof insulating or glasshouse, in case of strong sunlight need to enter
Row appropriateness shading, night uses the depth for keeping nutrient solution in water planting bottle during fluorescent lamp supplementary light, water planting to be not less than 1cm,
If not enough, step need to be added in time（9）Described nutrient solution；A foliar fertilizer was sprayed every 7 days, the foliar fertilizer presses step
（9）The culture formula of liquid is prepared, and is once cleaned and changes nutrient solution within every 7~10 days；
（13）30~40 days needle-leaved bundles of water planting are taken root, and start to transplant when root length reaches 1~2cm：First in seedbed face before transplanting
The upper line-spacing by 18~20cm excavates ditch deep 2~3cm, and the thick pure thin river sands of 2cm are spread in ditch, and transplanting is drenched for first 1~2 day
River sand, need to carry out under moisturizing dark conditions during transplanting, an aperture is opened on fine sand with scuppit or waddy, rooted seedling is put into hole
It is interior, root system is unfolded and is combined closely with sandy soil, the depth of planting is that seedling root neck is slightly below native face, and spacing in the rows is 3~5cm, after having planted
Pour permeable immediately, transplanted after being truncated when root length is more than 5cm, put arch bamboo chip on seedbed surface after transplanting, cover film thermal insulating moisturizing；
（14）Strengthen management, the humidity of running check transplanting medium, after removing film after Survival rate of nursery stock, is transplanted to the pin in seedbed
Leaf beam starts to grow new root for 10 days or so, original water root be transformed into by white after brown, lignifying be gradually transformed into it is normal
Root system；
（15）Two months or so needle-leaved bundle germinating to seedling, after bud is unearthed, by field seedling raising method carry out weeding, apply fertilizer, water, disease
Pest control seedling management；
Step（2）Described in pruning method be：Primary lateral branches and two grades of sturdy side shoot leading shoots were blocked at spring upper one year life branch,
Other side shoots are wiped out at the blight wood of autumn growth upper one year, while wiping out the weak branch of disease branch.
2. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（7）Described in the sand bed built made with brick or plank, the wide 80~100cm of sand bed, deep 20~30cm, the needle
Beam neat bury in sand bed puts into some rows, and with a line it is bundled be connected to bury put, the needle-leaved bundle bury the line-spacing put for 8~
10cm。
3. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（8）The water planting frame bottom is highly that, at intervals of 80~100cm, the vial is cylindrical glass bottle between 50cm, two layers,
The internal diameter of the vial is 6~8cm, interior a height of 8~10cm.
4. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 3, it is characterised in that：The water
Silica-gel electrothermic plate is covered with training frame.
5. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（10）Described in soaking depth of the needle-leaved bundle root in carbendazim or Bravo solution be 1~2cm；The needle-leaved bundle is being taken root
Soaking depth in plain solution is 1~2cm, and what the carbendazim solution was selected is 50% carbendazim, 500 times of liquid, the Bravo
What solution was selected is 70% Bravo, 800 times of liquid, and the root element solution is NAA, IBA or 911 root elements that concentration is 50mg/L
Solution.
6. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（12）Described in control day temperature be 25~28 DEG C, the nocturnal temperature be 15~18 DEG C.
7. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（11）Described in the selection of plastic, thermal-insulation canopy having a good transport service, illumination is sufficient, it is ventilated, close to flatly building for water source
Height into, the roof insulating is that 2~3m, the length of side are 10~25m, and ceiling is the Simple double-layer vinyl house on arch or inclined-plane,
Heating, humidification, illuminator are provided with the roof insulating.
8. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 6 or 7, it is characterised in that：Step
Suddenly（12）Described in the shading time between the ︰ 00 of 11 ︰ 00~15, open shading screen and carry out appropriate shading, the night supplements light
It is 18 ︰ 00 to the ︰ 00 of next day 7 according to the time, uses intensity of illumination for 2000lx fluorescent lamp supplementary light.
9. a kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree according to claim 1, it is characterised in that：Step
（12）Described in keep medium depth be 1.0~1.5cm.
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| CN105325294A (en) * | 2015-10-23 | 2016-02-17 | 辽宁省林业科学研究院 | Pinus-contorta needle-leaved-bundle water culturing nutrient solution and method |
| CN105766583B (en) * | 2016-03-28 | 2018-09-28 | 烟台市林业科学研究所 | A kind of black Japanese red pine needle-leaved bundle water planting rooting method |
| CN109644848A (en) * | 2019-01-17 | 2019-04-19 | 黔东南苗族侗族自治州农业科学院 | A kind of control method of shaddock insect pest |
| CN112868516A (en) * | 2021-01-15 | 2021-06-01 | 深圳园林股份有限公司 | Rapid propagation method for woody plants in wetland |
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| C06 | Publication | ||
| PB01 | Publication | ||
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Inventor after: Xu Yezhou Inventor after: Du Chaoqun Inventor after: Yang Daigui Inventor after: Chen Huachao Inventor after: Fan Banghai Inventor after: Xu Xiuhuan Inventor after: Lei Qinghu Inventor after: Fang Lidan Inventor before: Xu Yezhou Inventor before: Du Chaoqun Inventor before: Huang Guowei Inventor before: Chen Huachao Inventor before: Liu Xing |
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