CN104620832A - Cutting propagation method for ancient platycladus orientalis - Google Patents

Cutting propagation method for ancient platycladus orientalis Download PDF

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CN104620832A
CN104620832A CN201510079301.2A CN201510079301A CN104620832A CN 104620832 A CN104620832 A CN 104620832A CN 201510079301 A CN201510079301 A CN 201510079301A CN 104620832 A CN104620832 A CN 104620832A
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cuttings
arbor
ancient
vitae
years
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CN104620832B (en
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常二梅
江泽平
刘建锋
许洋
魏黔春
赵育新
李迎超
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Research Institute of Forestry of Chinese Academy of Forestry
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Research Institute of Forestry of Chinese Academy of Forestry
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029—Receptacles for seedlings
    • A01G9/0299—Handling or transporting of soil blocks or seedlings

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a cutting propagation method for an ancient platycladus orientalis. The method includes the steps: sequentially using potassium permanganate, sodium lignite and hormone for treating cuttings of the ancient platycladus orientalis to obtain treated cuttings; subjecting the treated cuttings to cuttage and cultivation in a matrix to realize cutting propagation of the ancient platycladus orientalis. According to experimental results, the cutting propagation method has the advantages that survival rate in clonal propagation of the ancient platycladus orientalis is increased, rooting time of the ancient platycladus orientalis is shortened, and references are provided for research on clonal propagation of other ancient trees.

Description

Ancient arbor-vitae cuttage breeding method
Technical field
The present invention relates to biological technical field, particularly relate to ancient arbor-vitae cuttage breeding method.
Background technology
Arbor-vitae is long at China's cultivation history, life-span reaches the ancient arbor-vitae of centuries, even several thousand, and in the whole nation, all there is distribution in many areas, along with coercing of being subject to of its inside and outside of ancient tree age growth increases gradually, arbor-vitae in thousand arbor-vitae of especially more than 3000 has now entered the high length of time, how effectively tree vigo(u)r is very weak, clonal propagation arbor-vitae at advanced age, make it life forever continuous, be worth and show, become global Chinese's question of common concern, also become the problem that local forest department needs solution badly.The age of tree is larger, and taking root more, difficulty is from being in progress both at home and abroad, and in the cottage propagation of centuries ancient tree, have some researchs at present, such as, 600 years ginkgo cutting plantation are 8%-25%.The discovery during age of tree to be the taxus chinensis in northeast of 300 years, 500 years and 600 years be material carries out cuttage, along with the cuttings elite stand age of tree, the increase at branch age, rooting percent presents reduction trend.In addition also have report to the ginkgo of about 1300 and the cottage propagation of Chinese scholartree, but ancient Chinese scholartree cuttage does not survive.According to U.S.'s New York Times, cut after fringe is planted from the fox-brush pine of 4600 years old (Bristlecone pine) and but do not survive, the successful cottage propagation of the arbor-vitae ancient tree more than 1000 is still blank.
The age of tree is larger, more difficultly takes root, and the internal cause affecting cottage propagation has: the difference etc. of age effect, position effect, colony and individual regeneration capacity, affects cuttage and expands numerous external cause and mainly contain growth regulator kind and concentration, matrix, illumination, temp. and humidity etc.
Summary of the invention
An object of the present invention is to provide a kind of ancient arbor-vitae cuttage breeding method.
Method provided by the invention, comprises the steps A:
The cuttings deriving from ancient arbor-vitae is used potassium permanganate, sodium lignate and HORMONE TREATMENT successively, obtains the cuttings after processing; By the cuttage in matrix of the cuttings after described process, cultivating to taking root, realizing ancient arbor-vitae cottage propagation.
Ancient arbor-vitae is the arbor-vitae that the age of tree is greater than 100 years.
In said method, described the cuttings deriving from ancient arbor-vitae to be comprised the steps: by potassium permanganate, sodium lignate and hormone-treated method successively
1) cuttings deriving from ancient arbor-vitae is soaked in potassium permanganate solution, obtain cuttings after potassium permanganate process;
2) cuttings after described potassium permanganate process is soaked in the sodium lignate aqueous solution, obtain cuttings after sodium lignate process;
3) cuttings after described sodium lignate process is soaked in hormone solution, obtain the cuttings after processing.
In said method, described hormone is NAA and IBA;
Described hormone solution is by solute and solvent composition, and described solute is NAA and IBA.
Described hormone solution preparation method: after being dissolved with a small amount of ethanol by hormone, then be mixed to get hormone solution with water.
In said method, the potassium permanganate mass percentage in described potassium permanganate solution is 0.05%;
Lignin acid concentration in the described sodium lignate aqueous solution is 500-2000mg.L -1;
In described hormone solution, NAA and IBA concentration is 600-4000mg.L -1.
In said method, the sodium lignate of described ancient arbor-vitae and correspondence thereof and hormone concentration following 1)-4) in any one:
1) age of tree of described ancient arbor-vitae is 100 years or 300 years or 3000, and the lignin acid concentration derived from described in deriving from the described sodium lignate aqueous solution corresponding to the cuttings of ancient arbor-vitae is 2000mg.L -1; Derive from NAA and IBA concentration in described hormone solution corresponding to the cuttings of ancient arbor-vitae described in deriving from and be 1000mg.L -1;
2) age of tree of described ancient arbor-vitae is 700, and the lignin acid concentration derived from described in deriving from the described sodium lignate aqueous solution corresponding to the cuttings of ancient arbor-vitae is 500mg.L -1; Derive from NAA and IBA concentration in described hormone solution corresponding to the cuttings of ancient arbor-vitae described in deriving from and be 600mg.L -1.
In said method, step 2) in, to derive from the age of tree be the cuttings of 100 years or 300 years or ancient arbor-vitae in 3000 is 2000mg.L in lignin acid concentration -1the sodium lignate aqueous solution in soak 1min,
Deriving from the age of tree is that the cuttings of 700 years ancient arbor-vitaes is at 500mg.L -1the sodium lignate aqueous solution in soak 2h,
Step 3) in, deriving from the age of tree is that the cuttings of 100 years or 300 years or ancient arbor-vitae in 3000 is 1000mg.L in NAA and IBA concentration -1hormone solution in soak 1min;
Deriving from the age of tree is that the cuttings of 700 years ancient arbor-vitaes is at 600mg.L -1solute be soak 1h in the hormone solution of NAA and IBA.
In said method, described in derive from ancient arbor-vitae cuttings be the raw branch of described ancient arbor-vitae tree crown sunny side top (position, tree crown top 3/1 to four/2nds) 1-3.
In said method, carry out disinfection to described cuttings in described cultivation, the bactericidal agent that described sterilization adopts is in methyl isophthalic acid H-2-benzimidazole carbamate, thiophanate-methyl, mancozeb, bordeaux mixture, thiophanate-methyl MnZn and ziram these 6 kinds at least two kinds.
Above-mentioned disinfection way is that 6 kinds of rotations use.
Another object of the present invention is to provide a kind of method of cultivating ancient arbor-vitae cuttage seeding.
Method provided by the invention, comprises the described steps A in above-mentioned cottage method
The age of tree of ancient arbor-vitae is 100 years or 300 years or 700 years or 3000.
Experiment of the present invention proves, this research makes full use of the arbor-vitae of all ages and classes, by comprehensively carrying out cottage propagation relation technological researching, explored through nearly 2 years, tentatively capture a Technique on Cuttage Propagation difficult problem, grope the cottage propagation system of a set of arbor-vitae ancient tree, and obtain the strain of arbor-vitae cuttage in 3000 clone seedling 11.This research is not only protection and clonal propagation arbor-vitae ancient tree is laid a good foundation, for other ancient tree vegetative propagations provide direction.
Ancient tree clonal propagation systems will create very large economic benefit to aspects such as the preservation of ancient tree excellent genes, culture technique results transferring, technical advice and trainings.The ancient tree excellent genes remained is for the breeding of the plant such as jujube tree, tea tree and genetic improvement and promote and can bring huge economic benefit to self-employed tree cultivator's industry again.Ancient tree has the background of deep culture, and the Xuanyuan cypress as Huang Di Mausoleum is called " father of world cypress " by Englishman, and ancient tree clonal propagation can preserve the complete genome of ancient tree, avoids Forest Culture and tourist culture legacy to disappear, significant.
Accompanying drawing explanation
Fig. 1 is different parts rooting percent.
Fig. 2 is cuttings Callus induction rate after mortifier is removed.
Fig. 3 is rooting percent after mortifier is removed.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
In following embodiment, the method for cuttage is as follows:
(1) cuttings is taked
2013-2014 clip with 5 years raw arbor-vitaes be contrast, on take the Temple of Heaven arbor-vitae and raw arbor-vitae (Huang Di Mausoleum Xuanyuan, Shaanxi cypress) tree crown middle and lower part, south in about 3000 3 years raw branches of 100 years, 300 years, 700 years the first round mitogenetic, growth selection stalwartness, branch (for cuttings) without damage by disease and insect, otch is encased with l Water Paper, spray water, bubble chamber is taken back.
(2) cuttings preparation and process
Be cut into about 20 cuttings (each 5 ~ 10cm) by each for the branch taken back, lower cut, from bud 1 ~ 1.5cm, is cut sth. askew, and otch should be level and smooth, at cuttings base portion 1 ~ 1.5cm place blade rip cutting 2 ~ 3 cutter, deeply reaches xylem, shears rear every 20 a bundles.Cuttings puts into the carbendazim solution leaching 15min of 1 ‰ immediately with sterilization, then cleans with clear water.Hormone is selected to soak.The cuttage degree of depth 8 ~ 10cm.Compacting matrix after inserting, and spray water 1 time with watering can immediately.
(3) matrix prepares
Peat: perlite=3:7
(4) slotting machine prepares
In Chinese forest-science academy, be provided with the circular slotting machine that radius is 6m, slotting machine is provided with intermittent spraying device, front thin rear close, often holds and puts 25, shower nozzle.Before cuttage 1d with 3 ‰ potassium permanganate cutting medium is carried out disinfection, cuttage clear water on the same day irrigates.
The cuttage fringe part of embodiment 1, ancient arbor-vitae is groped
One, the ancient arbor-vitae rooting percent of the different age of tree of hormon process
Growth hormone has the effect significantly promoting that adventive root and side root occur and grow, because the suitability of different somatotropin to cuttage rooting is inconsiderate, and different plant is also different to the adaptability of different somatotropin, therefore, the somatotropin of suitable kind is selected to seem particularly important to the cuttage of arbor-vitae ancient tree.Hormone is comparatively conventional these 3 kinds of hormones of kind ABT1, NAA and NAA:IBA=1:1 in selecting cuttage to test, and concentration design is divided into high concentration, middle concentration and low concentration.
ABT1 solution, NAA solution and NI solution (NAA:IBA=1:1) are according to different concentration preparation hormon solution, and concentration design is divided into low concentration (200-600mg.L -1; Shown in table 1), middle concentration (1000-3000mg.L -1; Shown in table 2) and high concentration (4000-10000mg.L -1; Shown in table 3).
With 5 years raw arbor-vitaes for contrast, the arbor-vitae of 100 years, 300 years, 700 years is tested as follows to take the Temple of Heaven:
In the aqueous solution (shown in table 1-3) containing hormon, 1-2h is soaked by after ancient for different age of tree arbor-vitae cuttings sterilization, then cuttage in matrix, cultivate.
After cultivating 45d, detect the total amount of the amount/cuttage cuttings of the callus rate=generation callus cuttings of the ancient arbor-vitae of the different age of tree;
After cultivating 90d, detect the total amount of the amount/cuttage cuttings of the rooting rate=cuttings of taking root of the ancient arbor-vitae of the different age of tree.
Result is as follows:
1, low concentration hormone is on the impact of arbor-vitae ancient tree cuttage root-taking
When using the low concentration HORMONE TREATMENT cuttings shown in table 1, result as table 1,5 years raw treelet 600mg.L -1aBT1 solution-treated 1h and 400mg.L -1nI solution (NAA:IBA=1:1) processes 1h all can reach good effect, and its Callus induction rate and rooting rate are 100%, and within 100 years, raw arbor-vitae is with 200mg.L -1aBT1 solution-treated 2h can have maximum Callus induction rate to be 18%, and final rooting rate is 12%.And 300 years and 700 years arbor-vitae cuttings are with 200mg.L -1nI solution (NAA:IBA=1:1) processes 4h, and Callus induction rate is 23% and 32% respectively, and rooting rate is 14% and 15%.
Table 1. low concentration hormone solution process all ages and classes arbor-vitae Callus induction rate, rooting rate
Note: A represents ABT1, N represents NAA, and NI represents NAA:IBA=1:1, and A200 represents that hormone concentration is 200mg.L -1aBT1, lower with, NI200 represents that the concentration of NAA and IBA in NI solution is 200mg.L -1.
2, middle concentration hormone is on the impact of arbor-vitae ancient tree cuttage root-taking
When using concentration HORMONE TREATMENT cuttings in shown in table 2, result is as table 2, and within 5 years, raw treelet Callus induction rate is all more than 50%, wherein uses 1000mg.L -1aBT1 solution-treated 1min and 1000mg.L -1nI solution-treated 30s all can reach good rooting efficiency, and its rooting rate is 88%, and the latter has higher Callus induction rate after treatment, is 100%.Within 100 years, raw arbor-vitae is with 2000mg.L -1nI solution-treated 3min and 3000mg.L -1nI solution-treated 1min Callus induction rate all reaches 33%, and rooting rate difference is little, is 19% and 18% respectively.Within 300 years, arbor-vitae cuttings is with 1000mg.L -1after NI solution-treated 30s, Callus induction rate is 48%, and rooting rate can reach 24%.And 700 years arbor-vitae cuttings are with 1000mg.L -1aBT1 solution-treated 1min effect is best, and have 20% cuttings to take root, 40% cuttings can break up callus.
Concentration HORMONE TREATMENT all ages and classes arbor-vitae Callus induction rate, rooting rate in table 2
Note: A represents ABT1, N represents NAA, and NI represents NAA:IBA=1:1, and A200 represents that hormone concentration is 200mg.L -1aBT1, lower with.
3, High concentration auxin is on the impact of arbor-vitae ancient tree cuttage root-taking
Use the High concentration auxin process cuttings of table 3, result is as shown in table 3,5 years raw treelets and 100 years arbor-vitae cuttings 8000mg.L -1during ABT1 solution-treated 5s, Callus induction rate is the highest, is respectively 99% and 67%, finally takes root, have notable difference, and within 5 years, raw treelet has 83% to take root, and within 100 years, then only has 18% to take root.300 years and 700 years arbor-vitae cuttings are then with 4000mg.L -1during NI solution-treated 5s, effect is better.
Table 3 High concentration auxin process all ages and classes arbor-vitae Callus induction rate, rooting rate
Note: A represents ABT1, N represents NAA, and NI represents NAA:IBA=1:1, and A200 represents that hormone concentration is 200mg.L -1aBT1, lower with.
Just all in all, when using High concentration auxin process cuttings, during its rooting efficiency does not use, the hormone-treated cuttings of concentration is good.
After hormones recipe determination, according to the experimental result tentatively drawn, the best root-inducing promoter being conducive to improving arbor-vitae at advanced age (especially the age of tree of 300 years and 700 years) cuttage rooting ability is NAA:IBA=1:1, further this combination formula is carried out refinement, by setting variable concentrations and processing time, match out best scheme further.
Two, NI different disposal is on the impact of cuttage rooting
With 5 years raw arbor-vitaes for contrast, the arbor-vitae of 100 years, 300 years, 700 years is tested as follows to take the Temple of Heaven:
At variable concentrations NI solution (NAA:IBA=1:1 after ancient for different age of tree arbor-vitae cuttings is sterilized; Table 4) the middle immersion 4h-1min time, then cuttage in matrix, cultivate.
Detect callus rate and the rooting rate of the ancient arbor-vitae of the different age of tree after four months, result is as table 4, and the best HORMONE TREATMENT concentration corresponding to all ages and classes cuttings is different.Wherein 5 years raw treelets and 100 years arbor-vitae cuttings are with 1000mg.L -1nI solution-treated 2min Callus induction rate is respectively 90% and 67%, and rooting rate is 79% and 25%.Within 300 years, arbor-vitae cuttings is then with 1000mg.L -1nI solution-treated 1min reaches higher rooting rate, and be 20%, and the Callus induction rate of 700 years arbor-vitae cuttings reaches 53%, the hormone used is 600mg.L -1nI solution-treated 1h, the highest rooting rate reaches 16%.
Table 4 variable concentrations NI solution-treated all ages and classes rooting percent
Can find out, 1000mg.L -1(NAA and IBA is 1000mg.L to NI solution -1), be 100 years, the NI concentration of 300 arbor-vitae the bests, for 700 years, NAA:IBA concentration was 600mg.L -1, be the NI concentration of the best.
Consider the processing time, then select NAA:IBA to be 1000mg.L -1optimum N I concentration as ancient arbor-vitae is carried out following experiment and is groped.
Three, the impact of bar position on cuttage root-taking is adopted
The inserted part of cuttings on elite stand is different, and cell division capacity is different, and the content also difference to some extent promoting material and internal hormones and related enzyme of taking root, affects the generation of callus and the formation of adventive root, cause rootability different.It is generally acknowledged, from the cuttings that tree crown bottom is taked, because formed obstruction material is few, nutrition content is higher, so rooting rate is high.But also someone thinks tree crown is in optical position, and branch flushes, and bottom is owing to being covered, and branch is undergrowth often, and therefore branch rootability in bottom is not as top.
Take on 100 years arbor-vitae east, the Temple of Heaven, Beijing, under east, Nan Shang, go down south, on west, under west, go up north, the cuttings in lower 8 orientation, north, and select concentration 1000mg.L after sterilization -1(NAA and IBA is 1000mg.L to NI solution -1) process 1min, then cuttage in matrix, cultivate (table 5).
Cuttage is after four months, and callus rate and rooting rate statistics are carried out in the cuttage experiment of difference being adopted to bar position.Result, as Fig. 1, be positioned at cuttings more easily the taking root compared with bottom cuttings on tree crown top, and the cuttings being positioned at elite stand the south is high compared with the Callus induction rate of other orientation cuttings, can reach 47%, and rooting rate is up to 42%.Therefore, when choosing cuttings, best to be positioned at top in the south (top 1/2 to 3/4 tree crown) cuttings.
Table 5 is for adopting the impact of bar position on cuttage root-taking
Four, endogenous Rooting is removed
Cuttings is more difficult takes root, and may take root and hinders material relevant with existing in body some.In general, the content of inhibitor can increase along with the increase of parental age, the corresponding rising along with the increase of shoot maturation degree.On same tree crown, the content of bottom inhibitor can be less than top inhibitor content.Inhibitor hinders the supposition of taking root of cuttings to have two kinds: one to be the effect weakening or stop growth hormone; Two is by being trapped in cuttings cut surface, as resin or tannin, thus affects the water suction of cuttings base portion.
A large amount of experiments shows, affects endogenous Rooting mainly phenols, flavonoids, tannin and the resin etc. of plant cuttage root-taking, and therefore mortifier removes experiment, carries out mainly for this several material, and in conjunction with Measure choice reset mode used in document.
HORMONE TREATMENT selects the good concentration 1000mg.L-1NI solution (NAA and IBA is 1000mg.L-1) of cuttage root-taking effect to process cuttings, study different mortifier reset mode to the impact of arbor-vitae cuttage, experimental design is as table 6, specific as follows:
With 5 years raw arbor-vitaes for contrast, the take the Temple of Heaven cuttings on top in the south of arbor-vitae tree crown of 100 years, 300 years, 700 years, after sterilization, be immersed in 0.1% silver nitrate aqueous solution, 0.1% aqueous acetic acid, 2% ethanol water, water, liquid detergent, 0.05% potassium permanganate solution different time respectively, use concentration 1000mg.L-1NI solution (NAA, IBA and water mixing again, obtain the aqueous solution, the final concentration of NAA and IBA is made to be 1000mg.L-1) process 1min, cuttage in matrix again, cultivates.
Cuttage, after four months, is investigated cuttage rooting situation, and its result such as Fig. 2 and Fig. 3, Fig. 2 are Callus induction rate result, and Fig. 3 is that mortifier removes result.Can find out, insert with 0.1% silver nitrate process, 5 years raw treelets
Fringe, its Callus induction rate can be made to reach 90%, and rooting rate reaches 62%.And with regard to 100 years, 300 years and 700 years arbor-vitae cuttings, use 0.05%KMnO 4process then can reach good rooting efficiency, and Callus induction rate is respectively 37%, 43% and 36%, and rooting rate is respectively 20%, 17% and 12%.
Result shows, and all ages and classes, different mortifier reset modes have larger difference to the impact of arbor-vitae cuttage rooting.In general, with KMnO 4process is beneficial to the arbor-vitae cuttings calli induction and cuttage rooting that are greater than 100 years age of trees most, and 0.1% acetic acid process does not have obvious effect to the removing of cuttings mortifier.
Table 6 mortifier removes cuttage experiment
Five, plant growth regulator is on the impact of all ages and classes arbor-vitae cuttage root-taking
Certain nutriment is needed, mainly carbohydrate and nitrogen compound during cuttage rooting.By aqueous sucrose solution process cuttings, hestening rooting better effects if.In addition, sodium lignate (ASL), as a plant growth regulators, has and promotes plant wound heeling, cuttings root of hair, raising percentage of seedgermination and accelerate the effects such as seedling growth, and can improve the resistance of forest seedling.By certain density ASL preimpregnation cuttings, in certain concentration range, there is obvious facilitation to taking root.Therefore, contrived experiment probes into the best ASL concentration of applicable arbor-vitae cottage propagation, and with the situation of taking root after aqueous sucrose solution process, specific as follows:
With 5 years raw arbor-vitaes for contrast, the take the Temple of Heaven cuttings on arbor-vitae tree crown top in the south of 100 years, 300 years, 700 years, after sterilization, be immersed in for 0.05% potassium permanganate solution 15min time, use sodium lignate (ASL) aqueous solution of variable concentrations and 10% aqueous sucrose solution to soak different time more respectively, then use concentration 1000mg.L -1nI solution-treated 1min, then cuttage in matrix, cultivate.
After four months, add up cuttage rooting situation, its result is as table 7.
Table 7 variable concentrations ASL process arbor-vitae Callus induction rate, rooting rate
As can be seen from Table 7, with certain density ASL and saccharose treatment arbor-vitae cuttings, certain effect is had to its rooting efficiency.All ages and classes desired concn is different.Within 5 years, raw treelet is with 200mg.L -1process 4h can form more callus, and rooting rate is up to 93%.100 years arbor-vitae 1000mg.L -1aSL aqueous solution process 3min and 2000mg.L -1it is 45% that ASL aqueous solution process 1min obtains identical rooting rate, and the Callus induction rate of the latter is higher.Variable concentrations ASL process 300 years cuttings, little to its Rooting effect.And with regard to 700 years arbor-vitae cuttings, low concentration soaks for a long time and is more conducive to it and takes root.Sucrose also has certain age effect to the impact of cuttage rooting, but is beneficial to taking root of cuttings generally.
Consider matter of time, 100 years and 300 years arbor-vitaes select 2000mg.L -1aSL aqueous solution soaking 1min, within 700 years, arbor-vitae selects 500mg.L -1aSL aqueous solution soaking 2h.
Six, bactericidal agent screening
After cuttage, cuttings is vulnerable to virus infections and direct sunlight too much causes temperature too high and burns seedling dehydration death, therefore regularly does sterilization rooting treatment after cuttage.Bactericidal agent comprises: carbendazim, thiophanate methyl (thiophanate-methyl), mancozeb, bordeaux mixture, thiophanate-methyl MnZn, good fortune good fortune zinc etc., specific as follows:
Take the cuttings on the Temple of Heaven arbor-vitae tree crown of 100 years top in the south, after sterilization, be immersed in for 0.05% potassium permanganate solution 15min time, then use 2000mg.L -1aSL aqueous solution soaking 1min, then use concentration 1000mg.L-1NI solution-treated 1min, then cuttage in matrix, cultivate.Nurturing period, on every Mondays, pasteurised completely sterilizing is carried out in four pairs of nursery stocks and seedbed, until cuttage root-taking (rootage duration is that rootage duration is 3 months to the time growing first adventive root and stop from cuttage), disinfection way is following 1) or 2):
1) carbendazim:
The biochemical Co., Ltd of Zhejiang spring scenery; Product registration number: LS95505
Carbendazim (methyl isophthalic acid H-2-benzimidazole carbamate) dilutes 500 times, and 2g converts 1kg water, and on every Mondays, four respectively to spray once, every square metre is sprayed about 1kg solution.
2) 6 kinds of bactericidal agent wheels are used
Carbendazim (methyl isophthalic acid H-2-benzimidazole carbamate), thiophanate methyl (thiophanate-methyl), mancozeb, bordeaux mixture, first frost MnZn and these 6 kinds of bactericidal agent wheels of Fu Fu zinc (zine dimethyldithiocarbamate) are used, on every Mondays, four respectively spray once, the bactericidal agent of each sprinkling is different, the order adopted in the present embodiment is: spray thiophanate methyl (thiophanate-methyl) Monday, Thursday sprays mancozeb, second Monday sprays bordeaux mixture, second Thursday sprays bordeaux mixture, 3rd Monday sprays thiophanate-methyl, 3rd Thursday sprays MnZn and ziram (zine dimethyldithiocarbamate)
Thiophanate-methyl (thiophanate methyl SC): Longdeng Chemical Co Ltd, Jiangsu; Product registration number: PD20080138
Thiophanate methyl dilutes 500 times, and 2g converts 1kg water, and every square metre is sprayed about 1kg solution.
Mancozeb: Limin Chemical Co., Ltd.; Product registration number: PD20040011
Mancozeb dilutes 500 times, and 2g converts 1kg water, and every square metre is sprayed about 1-2kg solution.
Bordeaux mixture: Ying Sui Guang Nongzhensheng Chemical Co., Ltd. of Guangdong Province; Product registration number: LS20003F030332
Bordeaux mixture dilutes 1000 times, and 1g converts 1kg water, and every square metre is sprayed about 1-2kg solution.
First frost MnZn: Zhejiang Prov HeBen pesticide Chemical Co., Ltd; Product registration number: PD20086169
First frost MnZn dilutes 500 times, and 2g converts 1kg water, and every square metre is sprayed about 1-2kg solution.
Good fortune good fortune zinc (zine dimethyldithiocarbamate): Hebei Zan Feng biotechnology Co., Ltd;
Good fortune good fortune zinc dilutes 250 times, and 4g converts 1kg water, and every square metre is sprayed about 1-2kg solution.
Result is as shown in table 8, and draw after carrying out a large amount of bactericidal agent screening, multiple bactericidal agent uses the best results obtained in turn.
Table 8 is greenery rate after raw arbor-vitae sterilizing in 100 years
Embodiment arbor-vitae Technique on Cuttage Propagation system in 2,3000
Each process 20.Repeat 3 times.
Arbor-vitae in 3000 due to the age of tree larger, cottage propagation is comparatively difficult, utilizing all ages and classes arbor-vitae cottage propagation to match out on the basis of good hormone combination and cuttings process, inquiring into the Technique on Cuttage Propagation system of Xuanyuan cypress further, making its reproductive success to utilizing cuttage mode.Good hormone ABT1 and NAA:IBA (1:1) in the above results is selected to carry out cuttage to Xuanyuan cypress in cuttage process.In cuttage process, cuttings growing state is observed, with rooting rate quantity for index, specific as follows:
Within 2013, take the cuttings on arbor-vitae (Xuanyuan, the Shaanxi cypress of Baidupedia or " in tree, the elderly person for whom a birthday celebration is being held knows how many ", " the many wonders of China ancient tree ") tree crown of 3000 in different month top in the south, after sterilization, be immersed in for 0.05% potassium permanganate solution 15min time, then use 2000mg.L -1aSL aqueous solution soaking 1min, then variable concentrations hormon solution-treated in table 9 respectively, then cuttage in matrix, cultivate.Nurturing period, on every Mondays, four pairs of nursery stocks and seedbed carry out pasteurised completely sterilizing, until cuttage root-taking, disinfection way is that several bactericidal agents wheels that in embodiment 1, table 8 adopts are used.Result is as table 9 and table 10.
Table 9 arbor-vitae rooting rate in 3000
Table 10 arbor-vitae cuttage system for handling in 3000
Can see in experimentation, the system being conducive to arbor-vitae cutting survival in 3000 is: take June 3000 arbor-vitae (Huang Di Mausoleum Xuanyuan cypress) raw then healthy and strong coppice shoot NAA:IBA (1:1) 1000mg.L of southern middle and upper part -1the rooting rate of arbor-vitae process 1min in 3000 is up to 2.5%, in the meantime, by being regularly cuttings sterilization, and carries out the control and management of moisture, temperature.Under above-mentioned system condition, to in November, 2013, existing 11 cuttings are successfully taken root and well-grown (having done 1000 cuttings altogether), at present the strain of survival seedling 6, average height 16.75cm, plant height is respectively: 27cm, 23cm, 19.5cm, 14cm, 11cm, 6cm.

Claims (9)

1. an ancient arbor-vitae cuttage breeding method, comprises the steps A:
The cuttings deriving from ancient arbor-vitae is used potassium permanganate, sodium lignate and HORMONE TREATMENT successively, obtains the cuttings after processing; By the cuttage in matrix of the cuttings after described process, cultivating to taking root, realizing ancient arbor-vitae cottage propagation.
2. method according to claim 1, is characterized in that:
Described the cuttings deriving from ancient arbor-vitae to be comprised the steps: by potassium permanganate, sodium lignate and hormone-treated method successively
1) cuttings deriving from ancient arbor-vitae is soaked in potassium permanganate solution, obtain cuttings after potassium permanganate process;
2) cuttings after described potassium permanganate process is soaked in the sodium lignate aqueous solution, obtain cuttings after sodium lignate process;
3) cuttings after described sodium lignate process is soaked in hormone solution, obtain the cuttings after processing.
3. method according to claim 1 and 2, is characterized in that: described hormone is NAA and IBA;
Described hormone solution is by solute and solvent composition, and described solute is NAA and IBA.
4. method according to claim 3, is characterized in that:
Potassium permanganate mass percentage in described potassium permanganate solution is 0.05%;
Lignin acid concentration in the described sodium lignate aqueous solution is 500-2000mg.L -1;
In described hormone solution, NAA and IBA concentration is 600-4000mg.L -1.
5. method according to claim 4, is characterized in that:
The sodium lignate of described ancient arbor-vitae and correspondence thereof and hormone concentration following 1) or 2):
1) age of tree of described ancient arbor-vitae is 100 years or 300 years or 3000, and the lignin acid concentration derived from the described sodium lignate aqueous solution corresponding to the cuttings of described ancient arbor-vitae is 2000mg.L -1; Derive from NAA and IBA concentration in described hormone solution corresponding to the cuttings of described ancient arbor-vitae and be 1000mg.L -1;
2) age of tree of described ancient arbor-vitae is 700 years, and the lignin acid concentration derived from the described sodium lignate aqueous solution corresponding to the cuttings of described ancient arbor-vitae is 500mg.L -1; Derive from NAA and IBA concentration in described hormone solution corresponding to the cuttings of described ancient arbor-vitae and be 600mg.L -1.
6., according to described method arbitrary in claim 2-5, it is characterized in that:
Step 2) in, to derive from the age of tree be the cuttings of 100 years or 300 years or ancient arbor-vitae in 3000 is 2000mg.L in lignin acid concentration -1the sodium lignate aqueous solution in soak 1min,
Deriving from the age of tree is that the cuttings of 700 years ancient arbor-vitaes is at 500mg.L -1the sodium lignate aqueous solution in soak 2h,
Step 3) in, deriving from the age of tree is that the cuttings of 100 years or 300 years or ancient arbor-vitae in 3000 is 1000mg.L in NAA and IBA concentration -1hormone solution in soak 1min;
Deriving from the age of tree is that the cuttings of 700 years ancient arbor-vitaes is at 600mg.L -1solute be soak 1h in the hormone solution of NAA and IBA.
7., according to described method arbitrary in claim 1-6, it is characterized in that: described in derive from ancient arbor-vitae cuttings be the raw branch of described ancient arbor-vitae tree crown sunny side top 1-3.
8. according to described method arbitrary in claim 1-7, it is characterized in that: carry out disinfection to described cuttings in described cultivation, the bactericidal agent that described sterilization adopts is in these 6 kinds, methyl isophthalic acid H-2-benzimidazole carbamate, thiophanate-methyl, mancozeb, bordeaux mixture, thiophanate-methyl MnZn and Fu Fu zinc at least two kinds.
9. cultivate a method for ancient arbor-vitae cuttage seeding, comprise claim 1-8 arbitrary in described steps A.
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CN105145681A (en) * 2015-10-10 2015-12-16 北京阿格瑞斯生物技术有限公司 Corn compound spraying agent, preparation method, using method and application thereof
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CN105993789A (en) * 2016-06-07 2016-10-12 武汉市农业科学技术研究院林业果树科学研究所 North America holly tender branch cutting fast asexual propagation method
CN108093904A (en) * 2017-12-18 2018-06-01 山东省林业科学研究院 A kind of method using red beech perennial branch cutting propagation
CN108990572A (en) * 2018-08-18 2018-12-14 西南林业大学 A kind of Gu raising seedlings by cuttage for Poplars
CN109220270A (en) * 2018-11-06 2019-01-18 北京林业大学 A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination
CN110800486A (en) * 2019-10-21 2020-02-18 中国林业科学研究院林业研究所 Method for improving ancient tree cuttage survival rate
CN110800486B (en) * 2019-10-21 2022-02-22 中国林业科学研究院林业研究所 Method for improving ancient tree cuttage survival rate
CN112369229A (en) * 2020-09-09 2021-02-19 北京市林业果树科学研究院 Cuttage propagation method for platycladus orientalis through electric heating hotbed in winter
CN115136801A (en) * 2022-07-18 2022-10-04 中国林业科学研究院林业研究所 Full-illumination spraying cuttage method for ancient arborvitae
CN115176609A (en) * 2022-07-18 2022-10-14 中国林业科学研究院林业研究所 Grafting method for ancient arborvitae

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