CN104604459A - Hard branch cutting propagation method of styrax japonicus seedlings - Google Patents
Hard branch cutting propagation method of styrax japonicus seedlings Download PDFInfo
- Publication number
- CN104604459A CN104604459A CN201310535214.4A CN201310535214A CN104604459A CN 104604459 A CN104604459 A CN 104604459A CN 201310535214 A CN201310535214 A CN 201310535214A CN 104604459 A CN104604459 A CN 104604459A
- Authority
- CN
- China
- Prior art keywords
- cuttings
- cuttage
- branch
- cutting
- propagation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a hard branch cutting propagation method of styrax japonicus seedlings. The method includes the following steps: (1) mixing pearlite and peat at a volume ratio of 1:1 to obtain a cutting substrate; (2) clipping strong branches without pest and disease damages from a strong seed tree of the styrax japonicus as seed branches, and performing cutting of the clipped hard branches after the beginning of spring, wherein the thicknesses of the branches is 0.3-0.5 cm; (3) cutting the hard branches into branch sections with lengths of 8-10 cm as cuttings using pruning shears, wherein each branch section has 2-3 buds; (4) soaking the cuttings in ABT rooting powder or a naphthalene acetic acid (NAA) solution; (5) performing cutting of the treated cuttings into a cutting bed (two-thirds of the cuttings into the soil), opening a spraying system, and watering the cutting bed thoroughly, so as to make the base parts of the cuttings contact with the substrate; and (6) watering thoroughly to make the cuttings contact with the substrate completely after the cutting is performed. The method has the advantages of strong operability, good treatment effect and wide market prospects and promotion values, and is beneficial to expand propagation of elite strains of the styrax japonicus.
Description
Technical field
The present invention relates to a kind of method for propagation, be specifically related to the cuttage propagation method of six directions snowball nursery stock, belong to phytobiology field.
Background technology
Six directions snowball is Guo Xing bio tech ltd, Jiangsu by the experimental study of 5 years, the superior type selected from Styrax tonkinesis (Styrax tonkinensis) natural population, be characterized in weather and the soil condition that can adapt to In Nanjing, the superior families nursery stock cultivated in Liuhe District, Nanjing at present grows fine.The six directions snowball selected from Styrax tonkinesis natural population not only cold resistance is strong, and serves many purposes.Six directions snowball seed oil content up to 70%, and containing the necessary oleic acid of a large amount of human body and linoleic acid, is not only the good raw material of processing biological diesel-oil, and is had very high edibility; Six directions snowball fast growth, 5-6 is a cycle of rotational cutting, and cut down rear regeneration power strong, biological yield is high, and its limb not only can be used as desirable technique, plywood and paper pulp material raw material, also can be used as the raw material producing bio-ethanol; Six directions snowball resin is containing balsamic acid, and can be used as valuable medicinal, is a kind of rising medicinal plant; Six directions snowball trunk is logical straight, and the dark green back of the body in blade face is white, and flower is like jasmine, and white bunchiness, delicate fragrance is also a kind of excellent green tree species.
Because six directions snowball natural resources are less, and seed has hibernation feature, and reproductive number is limited, constrains the development of six directions snowball Nursery Stock Industry to a certain extent.In order to improve the reproduction speed of six directions snowball nursery stock, and for the expanding propagation of improved seeds provide economical, Propagation Methods fast, carry out the research of six directions snowball nursery stock cottage propagation very necessary.But the domestic and international research report to the breeding of six directions snowball nursery stock cuttage is also very rarely seen at present.
In early days in research, applicant once have studied for this large kind of Styrax tonkinesis the method how improving its cottage propagation, and by research and development achievement application at that time patent (Chinese patent application 201210345280.0), but this kind of method is separately for the little kind of this seed selection of six directions snowball, due to the difference of botany growth characteristics, the method effect developed for Styrax tonkinesis of applicant bad at that time, be in particular in the kind bar choosing method adopting Styrax tonkinesis, cut cuttings method, cuttings processing methods etc. can play certain inhibitory action to the cottage propagation of six directions snowball, cause the branch of final cottage propagation cannot at germination rate, rooting rate obtaining a good balance (adopts branch ratio that the final rudiment * of scheme is at that time taken root not higher than 40%, botany there is no the value of actual use).Meanwhile, the cuttage degree of depth of Styrax tonkinesis crosses the growth that deeply also significantly can suppress cottage propagation branch.
At present, still there is not the further investigation for the breeding of six directions snowball nursery stock cuttage.
Summary of the invention
For the defect of prior art, the invention discloses a kind of cuttage propagation method being exclusively used in six directions snowball nursery stock, applicant carried out a large amount of research experiment for six directions snowball and found the method for effectively carrying out cottage propagation, adopt the germination rate of the final cuttage of method of the present invention and rooting rate to meet the requirement of plant amount reproduction growth.
For achieving the above object, the present invention is achieved through the following technical solutions:
The cuttage propagation method of six directions snowball nursery stock, comprises the following steps: perlite and peat are mixed and made into cutting medium by the volume ratio of 1:1 by (1); (2) healthy and strong from clip the healthy and strong elite stand of six directions snowball, without the branch of damage by disease and insect as kind of a bar, described branch is slightly 0.3-0.5cm, and the hard branch of clip is carried out cuttage after the beginning of spring; (3) be cut into the long branch section of 8-10cm as cuttings with secateurs, described branch section has 2-3 bud; (4) cuttings sheared is placed in ABT root-inducing powder or NAA solution soaks; (5) the cuttings cuttage processed is entered bed, insert 2/3 of branch in soil, after cuttage completes, open spraying system, slotting machine is irrigated, cuttings base portion and matrix are touched; (6) cuttage is watered permeable after completing, and cuttings is fully contacted with matrix.
In order to prevent from existing in cutting medium to the harmful mushroom of six directions snowball spray or other compositions, by the step that the carbendazim of dilution 800 ~ 1000 times or the U.S. spray solution cutting medium of anthrax good fortune carry out disinfection to it before described step (1) also comprises cuttage.
Concrete, within first 10 days, above-mentioned work can be carried out in cuttage, At All Other Times, according to the concrete condition proper extension of plant or to shorten also be feasible.
In the present invention, step (2) plant the standard chosen of bar be from the healthy and strong elite stand of the raw six directions snowball of 2-5 clip 1-2 raw healthy and strong, after the beginning of spring, carry out cuttage as kind bar again without the branch of damage by disease and insect.
Drawing after the choosing of the above-mentioned plant age of tree applicant carried out great many of experiments, is the age of tree being most suitable for six directions snowball cuttage growth.
After the above-mentioned beginning of spring, just cuttage both can be that the hard branch stayed upper one year in early March clip after the beginning of spring carries out cuttage, also can be that the autumn end of upper one year cuts hard branch then, prune good after huskyly hide moisturizing, after the beginning of spring, carry out cuttage again.
Wherein, the upper cut of step (3) cuttings otch is vertical with branch, and the distance from bud is 1-2cm, and lower cut is cut into horse ear shape.
Smooth cut should be made smooth when cuttings.
In the present invention, step (4) adopts in 400mg/kg ABT root-inducing powder or 100mg/kg NAA solution, soaks 2h, and fringe base immerses solution deep and is about 3cm.Preferably, 400mg/kg ABT rooting powder solution is adopted.
In the present invention, the line-spacing of cuttings can suitably adjust, and in order to ensure that blade that cuttings is stayed is not overlapped, step (5) seeding row spacing is 5cm × 10cm.
In order to promote normal growth, the sprinkling method of step (6) is in 30 days after inserting, spray in every 30 minutes 1 time, each spray 1-2 minute.
Consider the higher problem of temperature at high noon, interval of spraying water high noon can suitably be shortened.In 30-60 days after cuttage, should ensure blade face to hang with the globule and without ponding in matrix.
Confirmed by practice, after adopting cuttage 1-2 month of method six directions snowball nursery stock of the present invention, acrial part is rudiment, and underground part branch produces callus and adventive root.
In order to better promote that it grows, the potassium dihydrogen phosphate of foliage-spray 1/1000 mass concentration weekly growing of adventive root and the formation of side root can be promoted after rudiment.
Method of the present invention, cuttage is after 60 days, and cuttage rooting survives, and rooting rate can reach 75%, and cuttage can be transplanted after 3 months.
Compared with prior art, not yet have the cuttage breeding method relating to this jasmine kind of six directions snowball in prior art, existing method is used for six directions snowball effect cannot be satisfactory.Method disclosed by the invention is a kind of concrete, workable, and the method that treatment effect is good, is conducive to the expanding propagation of six directions snowball improved seeds, has wide market prospects.
Embodiment
In the following embodiments, six directions snowball plant species used is in May, 2013 buying from Guo Xing bio tech ltd, Jiangsu, and the said firm starts this kind of merchandise sales in March, 2013.
Embodiment 1: adopt the following cottage propagation carrying out six directions snowball
(1) cutting medium is prepared: perlite and peat are mixed and made into cutting medium by the volume ratio of 1:1.On March 10th, 2013, i.e. first 10 days of cuttage, spray cutting medium with the carbendazim solution of dilution 800 ~ 1000 times and carry out disinfection to it;
(2) plant bar to choose: from 5 years healthy and strong elite stands of raw six directions snowball clip 2 years raw stalwartnesses, without the branch of damage by disease and insect as kind of a bar, branch is slightly 0.3-0.5cm.The hard branch that clip stayed upper one year March 20 after the beginning of spring carries out cuttage.
(3) cuttings is cut: be cut into the long branch section of 8-10cm as cuttings with secateurs, every section has 2-3 bud.Cuttings otch is smooth, and upper cut is vertical with branch, and the distance from bud is 1-2cm, and lower cut is cut into horse ear shape.
(4) cuttings process: every 50 of the cuttings sheared is bundled into, is placed in 400mg/kg ABT rooting powder solution, soaks 2h, and fringe base immerses solution deep and is about 3cm.
(5) cuttage: timely for the cuttings processed cuttage is entered bed, seeding row spacing one be 5cm × 10cm, insert 2/3 of branch in soil, after cuttage completes, open spraying system in time, slotting machine is irrigated, cuttings base portion and matrix are touched.
(6) management after inserting: in 30 days after inserting, spraying in every 30 minutes 1 time, each spray 1-2 minute, interval of spraying water high noon is suitably shortened.After 30 days, extend spray intervals gradually, overcast and rainyly not spray water.
On June 20th, 2013, namely insert latter 3 months, statistics cuttage survival rate.
Result: through statistics, rooting percent is 75%, and nursery stock grows fine.
Embodiment 2: adopt the following cottage propagation carrying out six directions snowball
(1) cutting medium is prepared: perlite and peat are mixed and made into cutting medium by the volume ratio of 1:1.On March 10th, 2013, i.e. first 10 days of cuttage, carry out disinfection to it with the U.S. spray solution cutting medium of the anthrax good fortune of dilution 800 ~ 1000 times;
(2) plant bar to choose: from 3 years healthy and strong elite stands of raw six directions snowball clip 1 year raw stalwartness, without the branch of damage by disease and insect as kind of a bar, branch is slightly 0.3-0.5cm.The hard branch that clip stayed upper one year March 20 after the beginning of spring carries out cuttage.
(3) cuttings is cut: be cut into the long branch section of 8-10cm as cuttings with secateurs, every section has 2-3 bud.Cuttings otch is smooth, and upper cut is vertical with branch, and the distance from bud is 1-2cm, and lower cut is cut into horse ear shape.
(4) cuttings process: every 50 of the cuttings sheared is bundled into, is placed in 100mg/kg NAA solution, soaks 2h, and fringe base immerses solution deep and is about 3cm.
(5) cuttage: timely for the cuttings processed cuttage is entered bed, seeding row spacing one be 5cm × 10cm, insert 2/3 of branch in soil, after cuttage completes, open spraying system in time, slotting machine is irrigated, cuttings base portion and matrix are touched.
(6) management after inserting: in 30 days after inserting, spraying in every 30 minutes 1 time, each spray 1-2 minute, interval of spraying water high noon is suitably shortened.After 30 days, extend spray intervals gradually, overcast and rainyly not spray water.
On June 20th, 2013, namely insert latter 3 months, statistics cuttage survival rate.
Result: through statistics, rooting percent is 70%, and nursery stock grows fine.
Meanwhile, applicant carries out cuttage after immersion 2h to various ABT, NAA, ABT+NAA solution of other variable concentrations to six directions snowball 1 year stiff stem cutting fringe, and after the growth of 90 days, carry out the germination rate added up and rooting rate, result is as shown in table 1 below:
The hard branch rooting percent of table 1 hormon process
As can be known from Table 1, between hormon process there is larger difference in the rooting rate of hard stem cutting fringe, and wherein best with the effect of ABT400mg/L process, rooting rate reaches 75%.Secondly be 100mg/kg NAA solution, rooting rate reaches 70%.
In the research process of six directions snowball kind, applicant also attempts the cuttage breeding method being used for Styrax tonkinesis in early days (specifically to adopt the embodiment 1 of 201210345280.0 Chinese patent application specification embodiment parts, the method of 2) for six directions snowball cottage propagation of the present invention, find due to the two many difference existed on biological characteristics, the cuttage breeding method of Styrax tonkinesis is used for its final rooting rate of six directions snowball not higher than 50%, repeating in enormous quantities in experiment, one can only be up to the level of 40%, practical application needs cannot be met.
Claims (7)
1. the cuttage propagation method of six directions snowball nursery stock, is characterized in that comprising the following steps: perlite and peat are mixed and made into cutting medium by the volume ratio of 1:1 by (1); (2) healthy and strong from clip the healthy and strong elite stand of six directions snowball, without the branch of damage by disease and insect as kind of a bar, described branch is slightly 0.3-0.5cm, and the hard branch of clip is carried out cuttage after the beginning of spring; (3) be cut into the long branch section of 8-10cm as cuttings with secateurs, described branch section has 2-3 bud; (4) cuttings sheared is placed in ABT root-inducing powder or NAA solution soaks; (5) the cuttings cuttage processed is entered bed, insert 2/3 of branch in soil, after cuttage completes, open spraying system, slotting machine is irrigated, cuttings base portion and matrix are touched; (6) cuttage is watered permeable after completing, and cuttings is fully contacted with matrix.
2. cuttage propagation method according to claim 1, is characterized in that the step carried out disinfection to it with carbendazim or the U.S. spray solution cutting medium of anthrax good fortune of dilution 800 ~ 1000 times before step (1) also comprises cuttage.
3. cuttage propagation method according to claim 1, it is characterized in that step (2) plant the standard chosen of bar be from the healthy and strong elite stand of the raw six directions snowball of 2-5 clip 1-2 raw healthy and strong, without the branch of damage by disease and insect as kind of a bar.
4. cuttage propagation method according to claim 1, it is characterized in that the upper cut of step (3) cuttings otch is vertical with branch, the distance from bud is 1-2cm, and lower cut is cut into horse ear shape.
5. cuttage propagation method according to claim 1, it is characterized in that step (4) adopts in 400mg/kg ABT root-inducing powder or 100mg/kg NAA solution, soak 2h, fringe base immerses solution deep and is about 3cm.
6. cuttage propagation method according to claim 1, is characterized in that step (5) seeding row spacing is 5cm × 10cm.
7. cuttage propagation method according to claim 1, is characterized in that the sprinkling method of step (6) is in after inserting 30 days, spray in every 30 minutes 1 time, each spray 1-2 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310535214.4A CN104604459A (en) | 2013-11-01 | 2013-11-01 | Hard branch cutting propagation method of styrax japonicus seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310535214.4A CN104604459A (en) | 2013-11-01 | 2013-11-01 | Hard branch cutting propagation method of styrax japonicus seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104604459A true CN104604459A (en) | 2015-05-13 |
Family
ID=53139338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310535214.4A Pending CN104604459A (en) | 2013-11-01 | 2013-11-01 | Hard branch cutting propagation method of styrax japonicus seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104604459A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106145A (en) * | 2016-06-12 | 2016-11-16 | 青岛农业大学 | A kind of method for in-vitro rapid propagation of snowball embryo Seedling stem section |
| CN106577227A (en) * | 2016-12-02 | 2017-04-26 | 四川中科水务科技有限公司 | Quick cutting propagation method of lythrum salicaria |
| CN107360848A (en) * | 2017-09-13 | 2017-11-21 | 安徽徽思远生态农业发展有限公司 | A kind of Jasmine is transplanted the method for cultivation |
| CN107493872A (en) * | 2017-08-22 | 2017-12-22 | 北京农学院 | A kind of thunberg lespedeza cuttage propagation method |
| CN112243714A (en) * | 2020-10-16 | 2021-01-22 | 上海植物园 | Hardwood cutting propagation method for magnolia sieboldii |
| CN117243102A (en) * | 2023-09-12 | 2023-12-19 | 南京林业大学 | Cultivation method of tokyo wild jasmine |
-
2013
- 2013-11-01 CN CN201310535214.4A patent/CN104604459A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106145A (en) * | 2016-06-12 | 2016-11-16 | 青岛农业大学 | A kind of method for in-vitro rapid propagation of snowball embryo Seedling stem section |
| CN106106145B (en) * | 2016-06-12 | 2022-04-15 | 青岛农业大学 | In-vitro rapid propagation method for wild jasmine embryo seedling stem section |
| CN106577227A (en) * | 2016-12-02 | 2017-04-26 | 四川中科水务科技有限公司 | Quick cutting propagation method of lythrum salicaria |
| CN107493872A (en) * | 2017-08-22 | 2017-12-22 | 北京农学院 | A kind of thunberg lespedeza cuttage propagation method |
| CN107360848A (en) * | 2017-09-13 | 2017-11-21 | 安徽徽思远生态农业发展有限公司 | A kind of Jasmine is transplanted the method for cultivation |
| CN112243714A (en) * | 2020-10-16 | 2021-01-22 | 上海植物园 | Hardwood cutting propagation method for magnolia sieboldii |
| CN117243102A (en) * | 2023-09-12 | 2023-12-19 | 南京林业大学 | Cultivation method of tokyo wild jasmine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103039277B (en) | Bark grafting method of apocarya | |
| CN104585007B (en) | The cuttage and seedling culture method of American red-maple | |
| CN103931473B (en) | A kind of method spending the efficient Fast-propagation of tomonea in vain | |
| CN104322361B (en) | The method of a kind of Shandong wax No. 5 hardwood cutting and seedling raisings | |
| CN102783368B (en) | White kiwi breeding and cultivating technology | |
| CN104604459A (en) | Hard branch cutting propagation method of styrax japonicus seedlings | |
| CN103988697A (en) | Method for propagating apocarya seedlings | |
| CN102860204A (en) | Cuttage propagation method for styrax tonkinensis | |
| CN103749137A (en) | Cutting seedling method for malus toringoides | |
| CN101530041B (en) | Drupaceous economic forest rootstock hardwood cutting propagation method | |
| CN103609295A (en) | Method for large-scale cultivation of jatropha curcas seedlings | |
| CN101156551B (en) | Dendrobium nobile stem segment cuttage method | |
| CN105145071A (en) | Cutting propagation method of brucea javanica | |
| CN105519362A (en) | Method for grafting macadamia nuts | |
| CN106358903A (en) | High-yield pomegranate planting method | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN104737761B (en) | The cuttage breeding method of sinojackia xylocarpa | |
| CN102893811A (en) | Walnut grafting method | |
| CN103918430B (en) | A kind of styrax tonkinensis Craib ex Hart cuttage breeding method | |
| CN108925265A (en) | A kind of charming cuckoo cuttage breeding method of high mountain greening | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN103392491B (en) | Method for cutting seedling of Zenia insignis through adopting quarter sawing process | |
| CN102696370A (en) | Twig cutting method of Lycium Chinense Mill. of variety Mengqi No.1 | |
| CN103703997B (en) | Reproduction method for improving eucalyptus cutting rooting rate and scion strip utilization rate | |
| CN105830708A (en) | Cutting seedling raising method for acer truncatum bunge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150513 |