CN105993789A - North America holly tender branch cutting fast asexual propagation method - Google Patents
North America holly tender branch cutting fast asexual propagation method Download PDFInfo
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- CN105993789A CN105993789A CN201610398794.0A CN201610398794A CN105993789A CN 105993789 A CN105993789 A CN 105993789A CN 201610398794 A CN201610398794 A CN 201610398794A CN 105993789 A CN105993789 A CN 105993789A
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- 238000005520 cutting process Methods 0.000 title claims abstract description 108
- 235000003332 Ilex aquifolium Nutrition 0.000 title abstract 5
- 235000002294 holly Nutrition 0.000 title abstract 5
- 235000002296 holly Nutrition 0.000 title abstract 5
- 241000209027 Ilex aquifolium Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000002023 wood Substances 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 238000005286 illumination Methods 0.000 claims abstract description 6
- 240000003972 Ilex verticillata Species 0.000 claims description 37
- 235000003338 Ilex verticillata Nutrition 0.000 claims description 37
- 239000002689 soil Substances 0.000 claims description 35
- 239000000243 solution Substances 0.000 claims description 24
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 14
- 241000607479 Yersinia pestis Species 0.000 claims description 13
- 201000010099 disease Diseases 0.000 claims description 13
- 239000006013 carbendazim Substances 0.000 claims description 12
- 230000000249 desinfective Effects 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 238000010899 nucleation Methods 0.000 claims description 9
- 230000001954 sterilising Effects 0.000 claims description 9
- 239000003337 fertilizer Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 7
- 229940088597 Hormone Drugs 0.000 claims description 6
- VZJVWSHVAAUDKD-UHFFFAOYSA-N Potassium permanganate Chemical compound [K+].[O-][Mn](=O)(=O)=O VZJVWSHVAAUDKD-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000005556 hormone Substances 0.000 claims description 6
- 239000002985 plastic film Substances 0.000 claims description 6
- 238000009333 weeding Methods 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 230000003020 moisturizing Effects 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 230000000717 retained Effects 0.000 claims description 4
- 239000003448 gibberellin Substances 0.000 claims description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 3
- 238000009412 basement excavation Methods 0.000 claims description 2
- 238000005056 compaction Methods 0.000 claims description 2
- 230000003203 everyday Effects 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 239000010409 thin film Substances 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N Carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims 3
- 241000209035 Ilex Species 0.000 abstract description 5
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000001737 promoting Effects 0.000 abstract 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 10
- 230000004083 survival Effects 0.000 description 9
- 210000003608 Feces Anatomy 0.000 description 8
- 239000010871 livestock manure Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-Naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- XHXUANMFYXWVNG-ADEWGFFLSA-N Menthyl acetate Chemical compound CC(C)[C@@H]1CC[C@@H](C)C[C@H]1OC(C)=O XHXUANMFYXWVNG-ADEWGFFLSA-N 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M Monopotassium phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 241000398309 Satyrium ilicis Species 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 230000010496 root system development Effects 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 235000006439 Lemna minor Nutrition 0.000 description 1
- 240000001474 Portulaca oleracea Species 0.000 description 1
- 235000001855 Portulaca oleracea Nutrition 0.000 description 1
- 240000007072 Prunus domestica Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000844 anti-bacterial Effects 0.000 description 1
- 230000001488 breeding Effects 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- XKYFHUXZPRFUTH-UHFFFAOYSA-N dipotassium;dioxido(dioxo)manganese Chemical compound [K+].[K+].[O-][Mn]([O-])(=O)=O XKYFHUXZPRFUTH-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000011068 load Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
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- 239000003415 peat Substances 0.000 description 1
- 230000002085 persistent Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000750 progressive Effects 0.000 description 1
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- 230000002786 root growth Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
Abstract
The invention belongs to the technical field of plant asexual propagation, and relates to a North America holly tender branch cutting fast asexual propagation method. The method comprises the steps that North America holly is collected inside a garden to be adopted as a seed tree for artificial management, protection and cultivation, and scion wood is collected and subjected to cutting into a cutting bed with a cutting medium. The illumination, the relative temperature and humidity and the water content of the medium inside the cutting bed are controlled, on the condition that seed tree sources are quite limited, a large number of good North America holly cutting seedlings can be rapidly obtained, and a foundation is laid for promoting North America holly industrial development.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to a kind of asexual Fast-propagation side of ilex verticillata epicormic branch cuttage
Method.
Background technology
Ilex verticillata is Aquifoliaceae Ilex evergreen broad-leaved dungarunga, is that Forestry Fruit Tree Science Inst., Wuhan City is from the U.S.
The hybridization Ilicis Purpureae kind introduced, tree-like well branch and leaf are fine and close, and the Fructus Pyracanthae phase is long, and sight is good.Suitable garden, lawn
Isolated planting, hedgerow planting and highway isolation belt greening, making potted landscape etc., horticultural use is extensive.
At present, the seeding and seedling raising of ilex verticillata, sprout and need 1～2 year, bud ratio is the lowest, and seedling growth is slow.
Therefore, in order to protect and multiply this species resource and realizing sustainable development and utilization, a kind of north rapidly and efficiently is explored
U.S. Ilicis Purpureae method for culturing seedlings shortens growing-seedling period, thus advances the scale of ilex verticillata kind artificial propagation nursery, enters
And advancing the industrialization process of ilex verticillata, this is particularly important.
Finding according to retrieval, Wang Li English 2014 is its Master's thesis " ilex verticillata is bred and Landscape Application research "
In to disclose ilex verticillata optimization process be 500mg l-1IBA solution rooting rate may be up to 97.69%, but this examination
Test the most rigorous, not to thickness, length, the degree of lignification of cutting, take fringe position etc. and illustrate.
Zou Yi duckweed uses 1000mg l in 2014 in its Master's thesis " research of ilex verticillata fast breeding technique "-1IBA
Solution rooting rate reaches 68.75%, and its rooting rate is the lowest.
Summary of the invention
In view of the deficiencies in the prior art, it is an object of the invention to provide that a kind of cultivation period is short, the North America winter of low cost
Blue or green epicormic branch cuttage asexual rapid propagation method.By taking ilex verticillata epicormic branch cuttage asexual rapid propagation method of the present invention,
Under conditions of elite stand resource is extremely limited, it is possible to quickly obtain substantial amounts of excellent ilex verticillata cutting seedling, for advancing north
The industrialized development of U.S. Ilicis Purpureae lays the first stone.
In order to realize the purpose of the present invention, inventor passes through a large amount of creative experimental studies persistent exploration, eventually through
Gather ilex verticillata in garden, manually manage and protect cultivation as elite stand, gather tloig-cutlage in the cuttage having cutting medium
In bed, illumination in control cutting bed, relative humiture and water content of substrate, it is thus achieved that the most excellent ilex verticillata cuttage
Wood Seedling, its concrete technical scheme overview is as follows:
A kind of ilex verticillata epicormic branch cuttage asexual rapid propagation method, including elite stand manage and protect cultivation, cutting collection and process,
Managing operation after cuttage and cuttage, concrete operation step is as follows:
(1) elite stand manages and protects cultivation:
1. nursery lot selects: select that illumination is sufficient, soil is fertile, the convenient level land of irrigation and drainage or the gentle slope of gradient≤10 °
Ground is as ground, elite stand garden；
2. nursery lot turns over whole: entirely cultivate garden in advance, arranges furrow wide 1～1.5m, case high 0.1～0.2m, and soil is thin
Broken smooth, remove weeds and use sufficient base fertilizer；
3. elite stand gather: by the end of November～next year by the end of April, the locality healthy and strong North America without pest and disease damage of footpath more than 2cm
Ilicis Purpureae is standby as elite stand；The ilex verticillata of ground footpath≤5cm is with naked excavation；The ilex verticillata of ground footpath ＞ 5cm needs
Band soil ball, the diameter of soil ball is 6～10 times of footpath, elite stand ground；
4. elite stand plantation: planted on the same day that elite stand collection is returned or next day；1～2d with 0.5%～1% in advance
Carbendazim solution to plantation earth carry out disinfection, footpath, ground 2～5cm elite stand root with 0.5%～1% carbendazim soaking disinfection
5～10min, taking-up clear water rinses repeatedly, then collar is all stamped mud with lower portion, takes out after drying, by 2
× 1.5m seeding row spacing field planting, irrigates and determines root water；Footpath, ground ＞ 5cm elite stand, directly by 3 × 2m seeding row spacing field planting, irrigates and determines root
Water；
5. truncation promotees to sprout: in the elite stand field planting same day or next day, the branch of elite stand carries out truncation and promotees to sprout, and intercepted length is branch
The 1/4～1/3 of bar；
6. elite stand management: after elite stand field planting, root soil covered with plastic film, surrounding soil compaction seals；Elite stand moves
After planting 30d, execute an azophoska every 7d from root 20～30cm periphery；3～April, when elite stand starts to take out new
During bud, remove plastic sheeting；Adopt the elite stand after fringe carry out weeding and loosen the soil；
(2) cutting collection and process:
1. cutting collection: in mid-June～late August, on scion-seed tree, clip life, robust growth then are without disease pest
Evil, semi-lignified twig, branch diameter >=0.2cm, length 5～10cm is as cuttage cutting；
2. fringe bar is pruned: the cutting fringe bar of collection cuts off whole blades of more than base portion 3～4cm, only retains the leaf on top
Sheet 1～2, stay the 1/2～1/3 of full leaf to the blade retained, then cutting lower end be cut to angle shape, under cutting
Drawing 3～5 cuttves at end 0.5～1cm, the degree of depth reaches exactly to xylem；
3. disinfect: in disinfectant solution, soak cutting lower end, take out after sterilization 2～5min, stand-by after rinsing with clear water；
4. HORMONE TREATMENT: sterilization after cutting base portion in batch with 300～1000mg/L indole-3-butyric acid solution soaking
5～10s, the naphthalene acid solution with 300～800mg/L soaks 5～10s, then pulls out and drains, carries out cuttage immediately；
(3) cuttage:
1. cool canopy is built: making cutting bed in warmhouse booth or in the booth put up, cutting bed is put up before cutting propagation
Obscurity is that 50%～75% cool canopy is in case shading；
2. cutting time: in mid-June～late August；
(4) manage after cuttage:
1. cuttage management: the cutting after cuttage is set up shed immediately, shed is set up plastic film cover moisturizing, at plastics
Re-lay the sunshade net of obscurity 50%～75% on thin film, keep cutting medium water content 60%～70%, the wettest
Degree is maintained at more than 90%；The first half of the month of cuttage, observe the humidity of soil and the humidity of cutting bed every day；According to temperature
Humidity is watered, until taking root；Every 7d is to cutting seedling spray time carbendazim sterilization, until can transplant after taking root.
2. manage after taking root:
A, seedling exercising: after 15d of taking root, gradually decrease and water, reduce soil moisture and air humidity, carry out seedling exercising；
B, rush bud: after 15d of taking root, every 7d waters a Gibberellins solution, continuous pouring 2～4 times to root；
After c, seedling exercising manage: carry out the preventing and treating of rich water quality management and pest and disease damage, until Seedling grow tall 15～20cm time go out garden move
Plant.
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, wherein elite stand in step (1)
During plantation, described ABT3 or the ABT6 aqueous solution that mud concentration is 50～150mg/L and fresh gold zone
Soil dilution mixture forms, in starchiness.
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, wherein step (2) cutting is adopted
Collection: clip raw, robust growth then is without pest and disease damage, semi-lignified twig on scion-seed tree, branch diameter >=0.2cm,
Long 5～10cm as cuttage cutting.
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, the wherein cuttage of step (2)
Method is: drawing 3-5 cutter away from cutting lower end 0.5～1cm, the degree of depth reaches exactly to xylem.
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, the wherein cuttage of step (2)
Method is: sterilization after cutting base portion in batch with 300～1000mg/L indole-3-butyric acid solution soaking 5～10s,
Naphthalene acid solution with 300～800mg/L soaks 5～10s, then pulls out and drains, carries out cuttage immediately；
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, wherein step (3) cutting bed
Be set to edge of bed high 20～30cm, wide l～1.5m, long 6～15m；Bottom thickness 18-23cm in cutting bed
Clean fresh river sand or cobble make drainage blanket, upper strata is the plain red soil of thickness 10～20cm, and with 0.2%～0.5%
Potassium permanganate solution 2～3d carries out disinfection in advance.
Preferably, ilex verticillata green wood cutting asexual rapid propagation method as above, the wherein cuttage of step (3)
Method is: carry out cuttage by the seeding row spacing of 6 × 8cm on cutting bed, and the cuttage degree of depth is the 1/3～1/2 of cutting length,
Then the cutting medium around cutting is compacted；Water permeable double flushing blade after cuttage immediately, make cutting and cutting medium
Combine closely.
Compared with prior art, the ilex verticillata green wood cutting asexual rapid propagation method that the present invention relates to has following excellent
Point and progressive:
(1) ground, garden of female Miao Peiyu is to select outdoor plot, requires low to growing environment, simple to operate, convenient management；
Selected cutting medium raw material is easy to get, and propagation method is easily implemented.
(2) female Miao Yizhi taking root liquid and mixing with soil make mud, the viscous root of leaching, and female Miao Neng quickly recovers and grows
New root system, thus improve elite stand survival rate, promote it and sprout sprouting, grow 1～can sprout substantial amounts of after 3 months
Branch, shoot growth is rapid, and blade viridescent is dense, branch is healthy and strong, thus improves and adopt spike number amount.
(3) truncation promotees to sprout method is to block at 10～15cm bottom elite stand branch, can promote that elite stand sprouts newly quickly
Bud, branch also grows comparatively fast.
(4) requirement of fringe bar collection is that branch is about 5～10cm, shorter than the branch of general cottage propagation, can shorten fringe
The interval time that bar gathers, can effectively reduce cottage propagation material deterioration inside, fringe bar is in large supply, nursery in unit are
Quantity is many, land utilization ratio is high, improves reproductive efficiency.
(5) under conditions of elite stand resource is extremely limited, it is possible to quickly obtain substantial amounts of excellent ilex verticillata cutting seedling,
For advancing the industrialized development of ilex verticillata to lay the first stone.
(6) during cuttage, NAA and IBA is used together, promotes growth and the main root growth of fibrous root, and root system
The increase of quantity.
Detailed description of the invention
Following example further describe implementation process and the beneficial effect of the inventive method, and embodiment is only used for illustration
Purpose, does not limits the scope of the invention, simultaneously those of ordinary skill in the art according to the present invention done obvious
Change within being also contained in the scope of the invention.It addition, reagent of the present invention, wherein:
Gibberellins solution is configured to high concentration mother solution, i.e. 500mg/L when using.Concrete configuration method: weigh 50mg red
Mycin, first dissolves with a small amount of 95% ethanol, is then settled to 100mL with distilled water, can use.
The chemical composition of carbendazim is 2-benzimidazole ylcarbamic acid methyl ester, is the efficiently interior absorption of a kind of broad spectrum type low toxicity
Antibacterial, used in the present invention is 50% carbendazol wettable powder, is configured to high concentration mother solution, is during use
200 times of liquid.Weigh 1g carbendazim, be settled to 200ml with clear water, can use.
ABT3 is mainly used in nursery stock transplanting, promotes root system development, improves survival rate, increases anti-adversity ability.Concrete preparation
Method: put in nonmetallic vessel by 1gABT3 root-inducing powder, then adds 100～150ml ethanol dissolves, limit addition is stirred
Mix limit, after making root-inducing powder fully dissolve, add water and be diluted to suitable concentration used.
ABT6 is mainly used in nursery stock transplanting, promotes root system development, improves survival rate, increases anti-adversity ability.During configuration,
This root-inducing powder is directly dissolved in water.
Embodiment 1:
Illumination abundance, fertile soil, the convenient level land of irrigation and drainage or ground, gentle slope is selected to cultivate ground, garden as elite stand.Garden stage
Road width 40cm, furrow width 1.2m, the long 8m of furrow, dig trenches to drain the water away on the spot, and smooth remove impurity applies enough base manure simultaneously.Institute
Base manure proportioning raw materials be: fertilizer 90%, become thoroughly decomposed cake fertilizer 7% and azophoska 3%；Use base manure total amount for every mu
It is 800～1000kg.Mid or late October locality footpath 2～5cm the healthy and strong ilex verticillata without pest and disease damage as adopting
Fringe elite stand, the elite stand gathered be naked without mud, retain major part root system.The same day that female Seedling collection is returned plants
Plant.The healthy and strong ilex verticillata without pest and disease damage of ground footpath ＞ 5cm is as scion-seed tree, the elite stand band soil ball gathered, soil
Bulb diameter is 7 times of footpath, elite stand ground, and the same day that female Seedling collection is returned plants.First with 0.5% carbendazim solution pair
Footpath 2～5cm elite stand root soaking disinfection 10min taking-up clear water in ground rinses 3 times, then the root of female Seedling is all soaked viscous
On with the mud of taking root of the ABT3 hormone aqueous solution that concentration is 50mg/L Yu the freshest red soil dilution mixture
Slurry, takes out after drying by 2 × 1.5cm density field planting.After elite stand transplants 30d, execute one time 0.5% every 15d pouring and be combined
Rich water solution, and combine the work spraying microbial inoculum, weeding.Elite stand field planting same day, cut the branch to elite stand by profit and carry out
Truncation promotees to sprout, and is applied to compound fertilizer's aqueous solution that elite stand root concentration is 0.5%.
In late June, sprouting long more than the 6cm of branch when new, can gather cutting during semi-lignified, the fringe bar of collection is cut
Remove whole blades of the above 3cm of base portion, the blade 1 on a reservation top～2, the blade retained is cut off 1/3～1/2,
Again cutting lower end is cut to angle shape.Elite stand after adopting fringe is carried out weeding, loosens the soil and every strain applies the fertilizer become thoroughly decomposed
150g, calcium magnesium phosphate 150g, simultaneously every 10d concentration be 0.2% potassium dihydrogen phosphate and auximone spray tree body,
Repeatedly promote to sprout, take fringe for next cuttage and prepare.Gather fringe bar prune, in fringe bar base portion 1cm draw 3～
5 cuttves, the degree of depth reaches xylem.The cutting base portion sterilized is immersed in indole-3-butyric acid 500mg/L and naphthalene acetic acid in batch
In 500mg/L aqueous solution each 5～10s, pull out after air-dried 2～5min by the seeding row spacing cuttage of 6 × 8cm in being filled with skewer
Inserting in the cutting bed of substrate, cutting medium used is the freshest plain red soil；Cuttage is drenched after completing and is determined root water.
Cutting bed used be set to edge of bed height 30cm, wide lm, long 6m；Drainage blanket is made with clean fresh river sand in slotting machine,
Thickness 20cm, then loads cutting medium, thickness 10～15cm, and enters with 0.2% potassium permanganate solution 2d in advance
Row sterilization.Cutting after cuttage encircleing plastic film cover moisturizing immediately, keeps water content of substrate 60%, relative humidity is protected
Hold more than 90%.Spray 1 0.5% carbendazim every 7d, and add 0.2% potassium dihydrogen phosphate, spray after mix homogeneously
Cutting blade, until taking root.Period cutting to be purged in time if any drying up, go mouldy and falling leaves.Have after 60d
Take root on a small quantity appearance.The most every 7d investigates a secondary root situation.Complete survey survival rate after 90d, rooting rate reaches 95%,
Average every strain number of taking root is 37.Now carry out seedling exercising and reinforcement imposes root manure and the pipe such as foliage dressing, the prevention and control of plant diseases, pest control
Science and engineering is made, ready for outplanting.
Gather satisfactory branch to the last ten-days period in July and make second batch fringe bar, repeat the mistake after gathering first fringe bar
Journey.Complete survey survival rate after 90d after second batch tloig-cutlage, rooting rate reaches 92%, and average every strain number of taking root is
33.
Gather satisfactory branch to the last ten-days period in August and make the 3rd batch of fringe article, repeat the mistake after gathering first fringe bar
Journey.Complete survey survival rate after 90d after 3rd batch of tloig-cutlage, rooting rate reaches 90%, and average every strain number of taking root is
32.
Embodiment 2:
Select that illumination is sufficient, soil is more fertile, the convenient level land of irrigation and drainage is as ground, female Miao Peiyu garden.First garden is carried out
Quan Ken, divides seedbed and pavement, pavement width 40cm, furrow width 1.5m, the long 8m of furrow, digs trenches to drain the water away on the spot, smooth removes
Miscellaneous, apply enough base manure simultaneously.Base manure Material Percentage proportioning used is: every mu is used base manure total amount is 1000kg.
11～the healthy and strong ilex verticillata without pest and disease damage of December locality footpath more than 2cm life in 2 years as adopting fringe mother's Seedling, adopted
Female Seedling of collection be naked without mud, retain major part root system.The same day that female Seedling collection is returned plants.First with 0.5%
Female shoot root portion soaking disinfection 15～20min taking-up clear water is rinsed by carbendazim solution, then the root of female Seedling is all soaked
It is stained with the slurry of taking root with the ABT6 hormone aqueous solution that concentration is 50mg/L Yu the freshest yellow soil dilution mixture
(hormone water: the mass ratio of yellow soil is 1:3～4), takes out after drying by 30 × 45cm density field planting.Female Miao Yizhi
After 30d, every 15d Lin Shiyici 0.5% compound fertilizer aqueous solution, and combine the work spraying microbial inoculum, weeding.Female Seedling
Field planting same day, cut by profit and elite stand is carried out truncation promote to sprout, and be applied to compound fertilizer's aqueous solution that concentration is 0.5%.
In mid-June, sprout branch length 8～30cm, thick more than the 0.2cm in base portion footpath when new, can adopt during semi-lignified
Collection cutting, the fringe bar of collection cuts off whole blades of the above 2cm of base portion, and the blade 1 on a reservation top～2, to guarantor
The blade stayed slightly is cut short, then cutting lower end is cut to angle shape.Elite stand after adopting fringe is carried out weeding, loosens the soil.Adopt
The fringe bar of collection is pruned, and draws 3～5 cuttves in fringe bar base portion 1cm, and the degree of depth reaches xylem.The cutting base portion sterilized is become
Batch be immersed in indole-3-butyric acid 1000mg/L and naphthalene acetic acid 1000mg/L aqueous solution each 5～10s, pull out air-dried 2～
After 5min by the seeding row spacing cuttage of 6 × 8cm in the cutting bed being filled with cutting medium, cutting medium used is by body
Long-pending percentage ratio is that the peat soil three of 90% clean fresh yellow soil, 5% clean river sand and 5% is uniformly mixed；
Cuttage is drenched after completing and is determined root water.Cutting bed used be set to edge of bed height 30cm, wide l00cm, long 7m；In slotting machine
Make drainage blanket, thickness 20cm with clean fresh river sand, then load cutting medium, thickness 10cm, and high with 0.3%
Potassium manganate solution 2d in advance carries out disinfection.Cutting after cuttage is encircleed immediately plastic film cover moisturizing, keeps substrate aqueous
Amount 65%, relative humidity is maintained at more than 90%.Spray 1 0.5% carbendazim every 8d, and add 0.2% phosphoric acid
Potassium dihydrogen, sprays cutting blade, until taking root after mix homogeneously.Period cutting is wanted if any drying up, go mouldy and falling leaves
It is purged in time.60d has appearance of taking root on a small quantity.The most every 7d investigates a secondary root situation.Complete survey after 90d
Survival rate, rooting rate reaches 85.5%, and average every strain number of taking root is 35.Now carry out seedling exercising and reinforcement imposes root manure
Work is managed with foliage dressing, the prevention and control of plant diseases, pest control etc., ready for outplanting.
Gather second batch fringe bar to the last ten-days period in July, repeat the process after gathering first fringe bar.Second batch fringe bar skewer
Complete survey survival rate after 90d after inserting, rooting rate reaches 81.5%, and average every strain number of taking root is 32.
Gather satisfactory branch to the last ten-days period in August and make the 3rd batch of fringe article, repeat the mistake after gathering first fringe bar
Journey.Complete survey survival rate after 90d after 3rd batch of tloig-cutlage, rooting rate reaches 80.5%, and average every strain is taken root number
It it is 30.
Claims (7)
1. an ilex verticillata green wood cutting asexual rapid propagation method, including elite stand manage and protect cultivation, cutting collection and process,
Managing operation after cuttage and cuttage, concrete operation step is as follows:
(1) elite stand manages and protects cultivation:
1. nursery lot selects: select that illumination is sufficient, soil is fertile, the convenient level land of irrigation and drainage or the gentle slope of gradient≤10 °
Ground is as ground, elite stand garden；
2. nursery lot turns over whole: entirely cultivate garden in advance, arranges furrow wide 1～1.5m, case high 0.1～0.2m, and soil is in small, broken bits
Smooth, remove weeds and use sufficient base fertilizer；
3. elite stand gather: by the end of November～next year by the end of April, the locality healthy and strong North America without pest and disease damage of footpath more than 2cm
Ilicis Purpureae is standby as elite stand；The ilex verticillata of ground footpath≤5cm is with naked excavation；The ilex verticillata of ground footpath ＞ 5cm needs band
Soil ball, the diameter of soil ball is 6～10 times of footpath, elite stand ground；
4. elite stand plantation: planted on the same day that elite stand collection is returned or next day；1～2d with 0.5%～1% in advance
Carbendazim solution to plantation earth carry out disinfection, footpath, ground 2～5cm elite stand root with 0.5%～1% carbendazim soaking disinfection
5～10min, taking-up clear water rinses repeatedly, then collar is all stamped mud with lower portion, takes out after drying, by 2
× 1.5m seeding row spacing field planting, irrigates and determines root water；Footpath, ground ＞ 5cm elite stand, directly by 3 × 2m seeding row spacing field planting, irrigates and determines root
Water；
5. truncation promotees to sprout: in the elite stand field planting same day or next day, the branch of elite stand carries out truncation and promotees to sprout, and intercepted length is branch
The 1/4～1/3 of bar；
6. elite stand management: after elite stand field planting, root soil covered with plastic film, surrounding soil compaction seals；Elite stand moves
After planting 30d, execute an azophoska every 7d from root 20～30cm periphery；3～April, when elite stand starts to take out new
During bud, remove plastic sheeting；Adopt the elite stand after fringe carry out weeding and loosen the soil；
(2) cutting collection and process:
1. cutting collection: in mid-June～late August, on scion-seed tree, clip life, robust growth then are without disease pest
Evil, semi-lignified twig, branch diameter >=0.2cm, length 5～10cm is as cuttage cutting；
2. fringe bar is pruned: the cutting fringe bar of collection cuts off whole blades of more than base portion 3～4cm, only retains the leaf on top
Sheet 1～2, stay the 1/2～1/3 of full leaf to the blade retained, then cutting lower end be cut to angle shape, under cutting
Drawing 3～5 cuttves at end 0.5～1cm, the degree of depth reaches exactly to xylem；
3. disinfect: in disinfectant solution, soak cutting lower end, take out after sterilization 2～5min, stand-by after rinsing with clear water；
4. HORMONE TREATMENT: sterilization after cutting base portion in batch with 300～1000mg/L indole-3-butyric acid solution soaking 5～
10s, the naphthalene acid solution with 300～800mg/L soaks 5～10s, then pulls out and drain, carry out cuttage immediately；
(3) cuttage:
1. cool canopy is built: making cutting bed in warmhouse booth or in the booth put up, cutting bed is put up before cutting propagation
Obscurity is that 50%～75% cool canopy is in case shading；
2. cutting time: in mid-June～late August；
(4) manage after cuttage:
1. cuttage management: the cutting after cuttage is set up shed immediately, shed is set up plastic film cover moisturizing, at plastics
Re-lay the sunshade net of obscurity 50%～75% on thin film, keep cutting medium water content 60%～70%, relative humidity
It is maintained at more than 90%；The first half of the month of cuttage, observe the humidity of soil and the humidity of cutting bed every day；According to humiture
Water, until taking root；Every 7d is to cutting seedling spray time carbendazim sterilization, until can transplant after taking root.
2. manage after taking root:
A, seedling exercising: after 15d of taking root, gradually decrease and water, reduce soil moisture and air humidity, carry out seedling exercising；
B, rush bud: after 15d of taking root, every 7d waters a Gibberellins solution, continuous pouring 2～4 times to root；
After c, seedling exercising manage: carry out the preventing and treating of rich water quality management and pest and disease damage, until Seedling grow tall 15～20cm time go out garden transplant.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(1) in elite stand plantation time, described ABT3 or the ABT6 aqueous solution that mud concentration is 50～150mg/L with
Fresh yellow soil dilution mixture forms, in starchiness.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(2) when cutting gathers, clip raw, robust growth then is without pest and disease damage, semi-lignified twig, branch diameter >=0.2cm,
Length 5～10cm is as cuttage cutting.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(2) fringe bar is pruned: the cutting fringe bar of collection cuts off whole blades of more than base portion 3～4cm, only retains the blade on top
1～2, the blade retained is stayed the 1/2～1/3 of full leaf, then cutting lower end is cut to angle shape, from cutting lower end
Drawing 3～5 cuttves at 0.5～1cm, the degree of depth reaches exactly to xylem.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(2) HORMONE TREATMENT: sterilization after cutting base portion in batch with 300～1000mg/L indole-3-butyric acid solution soaking 5～
10min, the naphthalene acid solution with 300～800mg/L soaks 5～10min, then pulls out and drain, carry out cuttage immediately.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(3) cutting bed be set to edge of bed high 20～30cm, wide l～1.5m, long 6～15m；In cutting bed, bottom is thick
Clean fresh river sand or the cobble of degree 18-23cm make drainage blanket, and upper strata is the plain red soil of thickness 10～20cm, and uses
0.2%～0.5% potassium permanganate solution 2～3d carries out disinfection in advance.
Ilex verticillata green wood cutting asexual rapid propagation method the most according to claim 1, it is characterised in that: step
(3) cottage method is: carry out cuttage by the seeding row spacing of 6 × 8cm on cutting bed, and the cuttage degree of depth is cutting length
1/3～1/2, then the cutting medium around cutting is compacted；Water permeable double flushing blade after cuttage immediately, make cutting with
Cutting medium is combined closely.
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