CN104429596A - Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms - Google Patents
Method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms Download PDFInfo
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- CN104429596A CN104429596A CN201410641452.8A CN201410641452A CN104429596A CN 104429596 A CN104429596 A CN 104429596A CN 201410641452 A CN201410641452 A CN 201410641452A CN 104429596 A CN104429596 A CN 104429596A
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- silkworm
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- chinese caterpillar
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- caterpillar fungus
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 104
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 title abstract description 8
- 244000025254 Cannabis sativa Species 0.000 title abstract 5
- 238000000034 method Methods 0.000 claims abstract description 37
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 230000012447 hatching Effects 0.000 claims abstract description 6
- 241000233866 Fungi Species 0.000 claims description 36
- 240000001307 Myosotis scorpioides Species 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 238000002347 injection Methods 0.000 claims description 19
- 239000007924 injection Substances 0.000 claims description 19
- 241000190633 Cordyceps Species 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 235000013399 edible fruits Nutrition 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 240000000249 Morus alba Species 0.000 claims description 5
- 235000008708 Morus alba Nutrition 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 241000257303 Hymenoptera Species 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000007547 defect Effects 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000000249 desinfective effect Effects 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000009366 sericulture Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mushroom Cultivation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a method for producing silkworm insect grass by injecting cordyceps militaris to matured silkworms. The method includes the step of hatching and raising silkworm eggs, the step of disinfecting the matured silkworms, the step of infecting the silkworms, the step of injecting bacterium solution silkworms, the step of conducting illumination and temperature treatment on the injected silkworms and the step of treating the harvested silkworm insect grass. According to the method for producing the silkworm insect grass by injecting the cordyceps militaris to the matured silkworms, the matured silkworms are inoculated by the cordyceps militaris, the silkworm insect grass can be cultivated, and the method has the advantages of being simple in production process, high in success rate, low in cost, high in efficiency, capable of taking effect quickly and the like.
Description
Technical field
The present invention relates to Chinese caterpillar fungus and cultivate field, be specifically related to the method that a kind of matured silkworm injection cordyceps produces silkworm Chinese caterpillar fungus.
Background technology
Chinese caterpillar fungus is a kind of bacterium worm complex of the medicine-food two-purpose containing various bioactivators, has extensively long use history among the people, also the dark favor by Japan, Korea S, the every country in Southeast Asia people.The working condition very Special complex of natural cordyceps, output is rare and famous and precious, and digs due to artificial unordered Mining, and make natural cs increasingly in short supply, the protection of wild Chinese caterpillar fungus and the contradiction of the market demand are becoming increasingly acute.
For the ecotope of protection wild Chinese caterpillar fungus existence, government has put into effect to limit to dig or prohibit digs wild Chinese caterpillar fungus policy, and encourages scientific worker to research and develop other Chinese caterpillar fungus substitutes, to meet the need of market.
In more than 60 kind of domestic Chinese caterpillar fungus worm, composition, the pharmacological action of Cordyceps militaris are similar to natural cs, or even better.And it is relatively easily simple owing to researching and developing, be therefore listed in the type culture being suitable for most researching and developing Chinese caterpillar fungus substitute.
There are sericulture in more than 5000 year and the long history with silkworm in China, has abundant silkworm egg to provide former and sericulture experience.Select silkworm as the host of cordyceps, exploitation silkworm Chinese caterpillar fungus substitutes day by day rare Cordyceps sinensis, is obviously a wise selection.Inventor goes through the several years and to concentrate on studies exploration, have developed method of producing silkworm Chinese caterpillar fungus with ripe silkworm inoculation cordyceps of the present invention, the inventive method has the features such as production process is simple, success rate is high, cost is low, efficiency is high, instant effect, the silkworm people can be improved to breed silkworms added value, realize building up the family fortunes; The Chinese caterpillar fungus price that Chinese caterpillar fungus market is high can be stabilized again, meet the demand that people eat Chinese caterpillar fungus medicine.
Summary of the invention
In view of this, the invention provides the method that a kind of matured silkworm injection cordyceps produces silkworm Chinese caterpillar fungus, by with ripe silkworm inoculation cordyceps, realize the cultivation of silkworm Chinese caterpillar fungus, there is the features such as production process is simple, success rate is high, cost is low, efficiency is high, instant effect.
The invention provides the method that a kind of matured silkworm injection cordyceps produces silkworm Chinese caterpillar fungus, comprising:
Step 1: the hatching of silkworm seed and raising: silkworm seed is through ethanol disinfection 5min, and under temperature is 23 DEG C ~ 25 DEG C and humidity is the condition of 65% ~ 80%, hatching is collected ants, and raises to 6th ~ 8 days 5 ages voluntarily with mulberry leaf;
Step 2: the sterilization of matured silkworm: on superclean bench, get that described step 1 obtains 5 age matured silkworm dry up through ethanol disinfection;
Step 3: infecting of silkworm: draw bacterium liquid with sterilizing syringe, and to every bar silkworm injection 0.3 ~ 0.5ml;
Step 4: the process of injection bacterium liquid silkworm: the body surface liquid drying up the silkworm of injection bacterium liquid, put into desinfection chamber dark maintenance 20 ~ 25 days, maintenance condition is that temperature keeps 18 DEG C ~ 22 DEG C, and humidity keeps 75% ~ 90%, between defects liability period, the stiff long bacterium change of silkworm is checked, and reject body in time;
Step 5: light and temperature process: the silkworm after stiff is placed in daytime 20 ~ 24 DEG C, night the environment of 18 ~ 20 DEG C, promote sprouting and the growth of fruit body; Treat that polypide covers with orange-yellow mycelia and length and quantity orange-yellow fruit body (i.e. stroma) not etc., can gather in the crops;
Step 6: the process of the silkworm Chinese caterpillar fungus of results: carry out normal temperature to the silkworm Chinese caterpillar fungus of results and dry or be dry under the condition of 50 DEG C in temperature, after drying, pack sealing is kept in Dark Place.
Preferably, the concentration of the ethanol in described step 1 is 75%.
Preferably, in step 2 to 5 age matured silkworm carry out 2 ethanol disinfections and dry up.
Preferably, with the homogenizer of sterilizing, even filtration is carried out to bacterium liquid, then carry out injection use.
Preferably, described bacterium liquid is by being proceeded in normal potato liquid nutrient medium by Cordyceps militaris spawn, and jolts at dark 18 DEG C ~ 20 DEG C that 4 ~ 6d obtains.
Preferably, described sterilizing syringe adopts the syringe of the 5ml of No. 6 syringe needles.
Preferably, in step 3, from the postabdomen inserting needle of silkworm to neck, in silkworm body, inject bacterium liquid, extract syringe needle out on one side backward.
Preferably, in steps of 5, through 50d ~ 60d, polypide will cover with orange-yellow mycelia and length and quantity orange-yellow fruit body (i.e. stroma) not etc.
Preferably, in step 2, sterilization being dried up rear each bar matured silkworm is contained in plastic casing with cover stand-by respectively.
Preferably, rear silkworm will be dried up and split in plastic casing, then put into desinfection chamber.
As shown from the above technical solution, matured silkworm injection cordyceps provided by the invention produces the method for silkworm Chinese caterpillar fungus, by inoculating cordyceps with ripe silkworm, realize the cultivation of silkworm Chinese caterpillar fungus, there is the features such as production process is simple, success rate is high, cost is low, efficiency is high, instant effect, the silkworm people can be improved to breed silkworms added value, realize building up the family fortunes; The Chinese caterpillar fungus price that Chinese caterpillar fungus market is high can be stabilized again, meet the demand that people eat Chinese caterpillar fungus medicine.
Embodiment
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, clear, complete description is carried out below by the technical scheme in the embodiment of the present invention, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under the prerequisite not making creative work, all belongs to the scope of protection of the invention.
Embodiment:
The present embodiment material therefor comprises:
1.1, material: silkworm seed (cyanines pine X bright moon) is purchased from Shandong silkworm and mulberry institute.
1.2, cordyceps liquid: by by Cordyceps militaris spawn transferred species in normal potato liquid nutrient medium, under dark 20 DEG C of condition, jolt about 5d obtain.
1.3, medical 75% ethanol is commercial.
The method of the matured silkworm injection cordyceps production silkworm Chinese caterpillar fungus of the present embodiment comprises:
Step 1: the hatching of silkworm seed and raising: silkworm seed, through 75% ethanol disinfection 5min, is hatched and collected ants, raise voluntarily to 6th ~ 8 days 5 ages with mulberry leaf under 24 DEG C of temperature and humidity 80% conditions.
Step 2: the sterilization of matured silkworm: on superclean bench, get 269 5 age matured silkworm dry up through 2 ethanol disinfections, be contained in varying in size plastics with cover and closing stand-by respectively.
Step 3: bacterium liquid process: the even filtration of homogenizer of bacterium liquid sterilizing uses.
Step 4: infecting of silkworm: draw bacterium liquid with the 5ml sterilizing syringe of No. 6 syringe needles, from the postabdomen inserting needle of silkworm to neck, marginal not limit is taken out backward, every bar silkworm injection 0.3 ~ 0.5ml.
Step 5: the process of injection bacterium liquid silkworm: the silkworm of injection bacterium liquid, the liquid of silkworm body surface must be dried up, split former plastic casing Inner and put into desinfection chamber dark maintenance 20 ~ 25d, temperature keeps about 20 DEG C, humidity keeps about 90%, checks that the stiff long bacterium Bianization And of 1 silkworm rejects pollute corrupt silkworm body every 3d.
Step 6: light and temperature process: under the silkworm after stiff puts daytime 20 ~ 24 DEG C, night 18 ~ 20 DEG C of conditions, promotes sprouting and the growth of fruit body.About about 50d polypide covers with orange-yellow mycelia and length and quantity orange-yellow fruit body (stroma) not etc., can gather in the crops.
Step 7: the process of the silkworm Chinese caterpillar fungus of results: the silkworm Chinese caterpillar fungus of results is dried under 50 DEG C of conditions or normal temperature dries, pack sealing is kept in Dark Place.
The method of the present embodiment
Table one is for adopting the growth change of 10 box inoculation cordyceps silkworms of the method for the present embodiment.By table 1, often the distribution of box success rate is between 65% ~ 100% as seen, and average success rate is 89%.In existing method, the survival rate of producing silkworm Chinese caterpillar fungus with food silkworm is lower, and this method can reach 89% success rate, really through some effort, obtain not easily.
The growth change of table 1 10 box inoculation cordyceps silkworm
| Box number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Every summary |
| Silkworm number | 28 | 27 | 24 | 27 | 27 | 33 | 29 | 20 | 34 | 20 | 269 |
| Pollute number | 0 | 0 | 1 | 2 | 5 | 1 | 0 | 7 | 6 | 5 | 27 |
| Uncontaminated number | 28 | 27 | 23 | 25 | 22 | 32 | 29 | 20 | 28 | 15 | 242 |
| Synthesis power | 100 | 100 | 96 | 92 | 81 | 97 | 100 | 65 | 85 | 75 | 89 |
The silkworm Chinese caterpillar fungus of the maturation obtained according to the present embodiment is orange-yellow, and polypide covers with mycelia, and fruit body majority is grown thickly for bar-shaped, and there is ascus on top.The growth of silkworm Chinese caterpillar fungus is strong, and its fruit body is sturdy and quantity is also many, specifically in table 2:
The change of the weight of table 2 silkworm Chinese caterpillar fungus, length, fruit body length and quantity
It is to be noted: below 10mm fruit body is not in scope of statistics.
In sum, can think and can go out 242 silkworm Chinese caterpillar funguses with 269 food mulberry leaf silkworm injection cordyceps successful incubation, success rate is up to 89%; And then can think that the inventive method is reliably easy, success rate is high.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or equivalent replacement is carried out to wherein portion of techniques feature, and these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (10)
1. matured silkworm injection cordyceps produces a method for silkworm Chinese caterpillar fungus, and it is characterized in that, described method comprises:
Step 1: the hatching of silkworm seed and raising: silkworm seed is through ethanol disinfection 5min, and under temperature is 23 DEG C ~ 25 DEG C and humidity is the condition of 65% ~ 80%, hatching is collected ants, and raises to 6th ~ 8 days 5 ages voluntarily with mulberry leaf;
Step 2: the sterilization of matured silkworm: on superclean bench, get that described step 1 obtains 5 age matured silkworm dry up through ethanol disinfection;
Step 3: infecting of silkworm: draw bacterium liquid with sterilizing syringe, and to every bar silkworm injection 0.3 ~ 0.5ml;
Step 4: the process of injection bacterium liquid silkworm: the body surface liquid drying up the silkworm of injection bacterium liquid, put into desinfection chamber dark maintenance 20 ~ 25 days, maintenance condition is that temperature keeps 18 DEG C ~ 22 DEG C, humidity keeps 75% ~ 90%, between defects liability period, the stiff long bacterium change of silkworm is checked, and reject the silkworm body polluting corruption in time;
Step 5: light and temperature process: the silkworm after stiff is placed in daytime 20 ~ 24 DEG C, night the environment of 18 ~ 20 DEG C, promote sprouting and the growth of fruit body; Treat that polypide covers with orange-yellow mycelia and length and quantity orange-yellow fruit body (i.e. stroma) not etc., can gather in the crops;
Step 6: the process of the silkworm Chinese caterpillar fungus of results: carry out normal temperature to the silkworm Chinese caterpillar fungus of results and dry or be dry under the condition of 50 DEG C in temperature, after drying, pack sealing is kept in Dark Place.
2. method according to claim 1, is characterized in that, the concentration of the ethanol in described step 1 is 75%.
3. method according to claim 1, is characterized in that, in step 2 to 5 age matured silkworm carry out 2 ethanol disinfections and dry up.
4. method according to claim 1, is characterized in that, carries out even filtration with the homogenizer of sterilizing to bacterium liquid, then carries out injection use.
5. method according to claim 1, is characterized in that, described bacterium liquid be by by Cordyceps militaris spawn transferred species in normal potato liquid nutrient medium, and jolt under dark 18 DEG C ~ 20 DEG C conditions and to obtain for 4 ~ 6 days.
6. method according to claim 1, is characterized in that, described sterilizing syringe adopts the syringe of the 5ml of No. 6 syringe needles.
7. method according to claim 1, is characterized in that, in step 3, from the postabdomen inserting needle of silkworm to neck, in silkworm body, injects bacterium liquid, extracts syringe needle out on one side backward.
8. method according to claim 1, is characterized in that, in steps of 5, through 45 ~ 60 days, polypide will cover with orange-yellow mycelia and length and quantity orange-yellow fruit body (i.e. stroma) not etc.
9. method according to claim 1, is characterized in that, in step 2, sterilization is dried up rear each bar matured silkworm and is contained in plastic casing with cover stand-by respectively.
10. method according to claim 1, is characterized in that, will dry up rear silkworm and split plastic casing Inner, then put into desinfection chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410641452.8A CN104429596B (en) | 2014-11-13 | The method that matured silkworm injection Cordyceps militaris (L.) Link. fungus produces silkworm grass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410641452.8A CN104429596B (en) | 2014-11-13 | The method that matured silkworm injection Cordyceps militaris (L.) Link. fungus produces silkworm grass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104429596A true CN104429596A (en) | 2015-03-25 |
| CN104429596B CN104429596B (en) | 2017-01-04 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982225A (en) * | 2015-07-02 | 2015-10-21 | 溧阳市天目湖农业发展有限公司 | Novel male silkworm cordyceps culturing process and method for culturing silkworm cordyceps parent species |
| CN105706738A (en) * | 2016-02-23 | 2016-06-29 | 张家港顺泰元生物科技有限公司 | Cordycepsmilitaris cultivation method |
| CN106134758A (en) * | 2015-04-10 | 2016-11-23 | 北京银桥绿色农业科技有限公司 | Aseptic silkworm inoculation Cordyceps militaris (L.) Link. fungus cultivates silkworm grass method |
| CN106818211A (en) * | 2017-02-17 | 2017-06-13 | 四川久远生物技术有限公司 | A kind of method that utilization empty stomach silkworm cultivates silkworm grass |
| CN107494458A (en) * | 2017-08-15 | 2017-12-22 | 广西八园农业科技有限公司 | Method that is a kind of while producing flat board silk and the larva of a silkworm with batrytis |
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| CN101720627A (en) * | 2009-12-16 | 2010-06-09 | 江苏江南生物科技有限公司 | Method for culturing cordyceps militaris by living silkworm chrysalises |
| CN102550283A (en) * | 2010-12-30 | 2012-07-11 | 杨发国 | Technology for artificially breeding cordyceps sinensis by substituting silkworm pupae for hepialus armoricanus |
| CN103262751A (en) * | 2013-05-15 | 2013-08-28 | 刘东波 | Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae |
| CN103477871A (en) * | 2013-09-29 | 2014-01-01 | 浙江理工大学 | Cultivation method and application of cordyceps militaris |
| CN103688759A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
| CN103704022A (en) * | 2013-12-20 | 2014-04-09 | 凌中鑫 | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
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| CN101720627A (en) * | 2009-12-16 | 2010-06-09 | 江苏江南生物科技有限公司 | Method for culturing cordyceps militaris by living silkworm chrysalises |
| CN102550283A (en) * | 2010-12-30 | 2012-07-11 | 杨发国 | Technology for artificially breeding cordyceps sinensis by substituting silkworm pupae for hepialus armoricanus |
| CN103262751A (en) * | 2013-05-15 | 2013-08-28 | 刘东波 | Method for cultivating cordyceps militaris by using cordyceps militaris strains to infect silkworm larvae |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134758A (en) * | 2015-04-10 | 2016-11-23 | 北京银桥绿色农业科技有限公司 | Aseptic silkworm inoculation Cordyceps militaris (L.) Link. fungus cultivates silkworm grass method |
| CN104982225A (en) * | 2015-07-02 | 2015-10-21 | 溧阳市天目湖农业发展有限公司 | Novel male silkworm cordyceps culturing process and method for culturing silkworm cordyceps parent species |
| CN104982225B (en) * | 2015-07-02 | 2017-06-16 | 溧阳市天目湖农业发展有限公司 | The method of male silkworm grass cultivating technique and culture silkworm grass parent species |
| CN105706738A (en) * | 2016-02-23 | 2016-06-29 | 张家港顺泰元生物科技有限公司 | Cordycepsmilitaris cultivation method |
| CN106818211A (en) * | 2017-02-17 | 2017-06-13 | 四川久远生物技术有限公司 | A kind of method that utilization empty stomach silkworm cultivates silkworm grass |
| CN107494458A (en) * | 2017-08-15 | 2017-12-22 | 广西八园农业科技有限公司 | Method that is a kind of while producing flat board silk and the larva of a silkworm with batrytis |
| CN107494458B (en) * | 2017-08-15 | 2020-10-16 | 广西八园农业科技有限公司 | Method for simultaneously producing flat silk and white muscardine silkworm |
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