CN103766139A - Method for producing worm grass - Google Patents

Abstract

Healthy living barley pests of 4 years old to 6 years old are selected, 0.02-0.3ml spore reproduction fermenting mash containing worm grass hypha sporophytes is injected to a pest body close to a head by using a subcutaneous injection method, the pest body is put into a sterilized culture bottle, and worm grass with the barley pests as carriers can be obtained through temperature control, light control and humidity control.

Description

The production method of a kind of Chinese caterpillar fungus

Technical field

The present invention relates to the production method of a kind of Chinese caterpillar fungus.Relating in particular to a kind of existing polypide has again careless Chinese caterpillar fungus production method, belongs to edible mushroom field.

Background technology

[0002] northern Chinese caterpillar Fungus and Cordyceps sinensis, is referred to as Chinese caterpillar fungus in the present invention, is famous and precious nourishing material, can improve people's immunocompetence, along with the exhaustion day by day of wild resource, manually train clay and be the inevitable course, but at present, the medium that northern Chinese caterpillar Fungus uses is wheat or rice, only have grass, there is no worm shape, also have and use silkworm and silkworm chrysalis, but silkworm eats mulberry leaf, be difficult to cultivate continuously throughout the year.And the cultivation of the artificial bionic of Cordyceps sinensis is made nutrition base with bat moth larvae, this worm is very long vegetative period, and cultivation is wasted time and energy.The invention provides one and do nutrition base carrier with barley worm, it had both been applicable to northern Chinese caterpillar Fungus mycelial growth, was also applicable to Chinese caterpillar fungus bacterial filament growth, and can grows stroma body.

The invention has the beneficial effects as follows: the growth of barley worm is fast, feeding habits are assorted, food source is wide, can cultivate throughout the year, uses it as the medium of Chinese caterpillar fungus, cost is very low, and the existing worm shape of the Chinese caterpillar fungus of turning out, has again stroma body, and the utmost point is as wild Chinese caterpillar fungus, and because being live body cultivation, its effective caterpillar fungus nutritire component content of producing is all very high.

Summary of the invention

Current known culture medium of Cordyceps militaris has: rice, wheat, silkworm, silkworm chrysalis and bat moth larvae.The invention provides a kind of Chinese caterpillar fungus production method of making medium with barley worm, production process is: by Chinese caterpillar fungus test tube slant kind, press well-established law, obtaining shaking flask expands after breeding, in aseptic lucifuge, in 15 to 25 degrees Celsius of environment, leave standstill 7 to 15 days, select and above mash, have white hypha body layer, the expansion breeding that what mash was limpid contain thallospore body is for subsequent use.Then select the healthy live body barley worm in 4 to 6 ages, be placed in the environment of 1 to 5 degree Celsius 15 to 45 minutes, make it enter resting state, then spill seminal plasma wash disinfection 15 to 300 seconds with 75 degree, after airing polypide, in the situation that not injuring polypide brain and enteron aisle, by injection to polypide 0.02 to the 0.3 milliliter of above-mentioned for subsequent use expansion breeding mash that contains Chinese caterpillar fungus hypha sporophyte of hypodermic injection near head, then polypide is put into the blake bottle through sterilization, temperature is controlled to 10 to 25 degrees Celsius, relative moisture is 50% to 60% shading environment, through 5 to 10 days, visible polypide is stiff, in body, cover with Chinese caterpillar fungus hypha, then, temperature is controlled to 15 to 25 degrees Celsius, relative moisture is controlled at 75% to 85%, shine more than 10 hours every day with fluorescent lamp, through 30 to 70 days, when polypide stroma grows to 1 centimetre of left and right, take out low temperature drying and obtain the Chinese caterpillar fungus that barley worm is carrier.

Specific embodiment

By Chinese caterpillar fungus test tube slant kind, by well-established law, obtain shaking flask and expand after breeding, in aseptic lucifuge, in 20 degrees Celsius of environment, leave standstill 10 days, to select and above mash, have white hypha body layer, the expansion breeding that what mash was limpid contain thallospore body is for subsequent use.Then select the healthy barley worm in 6 ages, be placed in the environment of 3 degrees Celsius 30 minutes, make it enter resting state, then spill seminal plasma wash disinfection 30 seconds with 75 degree, after airing polypide, by injection to polypide the 0.1 milliliter of above-mentioned for subsequent use expansion breeding mash that contains thallospore body of hypodermic injection near head, then polypide is put into the blake bottle through sterilization, be placed in 15 to 18 degrees Celsius of temperature, relative moisture is 50% to 60% shading environment, through 7 days, pick out the polypide of feeling like jelly, the stiff polypide of only covering with Chinese caterpillar fungus hypha in surplus body, then, temperature is controlled to 15 to 25 degrees Celsius, relative moisture is controlled at 80% to 85%, shine 12 hours every day with fluorescent lamp, through approximately 45 days, Chinese caterpillar fungus stroma grows to 1 centimetre of left and right, take out low temperature drying and obtain the Chinese caterpillar fungus that barley worm is carrier.

Claims (1)

1. a production method for Chinese caterpillar fungus, is characterized in that: make medium with barley worm, select the healthy live body barley worm in 4 to 6 ages, by injection to polypide 0.02 to the 0.3 milliliter of mash that contains Chinese caterpillar fungus hypha sporophyte of hypodermic injection near head.