CN101878720A - Method for domesticating and cultivating wild mushroom - Google Patents
Method for domesticating and cultivating wild mushroom Download PDFInfo
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- CN101878720A CN101878720A CN2010102150700A CN201010215070A CN101878720A CN 101878720 A CN101878720 A CN 101878720A CN 2010102150700 A CN2010102150700 A CN 2010102150700A CN 201010215070 A CN201010215070 A CN 201010215070A CN 101878720 A CN101878720 A CN 101878720A
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Abstract
The invention discloses a method for domesticating and cultivating wild mushroom. The method comprises original seed decomposition, preparation of mother culture medium and inoculation of secondary and tertiary strains, and ensures the quality of the domesticated and cultivated wild mushroom by controlling temperature and humidity thereof. The method has the effects of overcoming the defects in the prior art, increasing the yield of the wild mushroom and improving the quality thereof.
Description
Technical field
The present invention relates to the cultivating method of mushroom, particularly the domesticating cultivation method of a kind of wild bacterium.
Background technology
Wild bacterium is a kind of natural, free of contamination organic food.Because wild bacterium is subjected to the restriction of season and temperature, and to have only some months time, reserves every year all be limited.Most of sales departments all use general edible mushroom to replace wild bacterium, but real wild bacterium is the organic food that supply falls short of demand on the market really.As the domestication of Cordyceps sinensis, Chinese caterpillar fungus is human indispensable traditional Chinese medicine, is the best product of health tonifying Qi, also is the famous and precious medicine that supply falls short of demand on the market.Exactly because so, wild bacterium domesticating and cultivating is just seemed particularly important, and the control of domesticating and cultivating kind temperature and humidity then is domesticating and cultivating kind most important parts.
Summary of the invention
The invention provides the domesticating cultivation method of a kind of wild bacterium, this method is carried out according to following steps:
A. original seed decomposes, and selects to physically well develop, maturity is that eight to ninety percent original seed decomposes, and the original seed after the decomposition is put into pre-prepd medium in vitro, observes and choose to grow preferably original seed and inoculate with mother culture media;
B. the preparation of mother culture media, at first, (1) the moyashi 500g for preparing to prepare burden in three minutes, liquor white sugar 20g, agar 20g, hickory chick footing soil 50g, water 1000ml; (2) wooden oak bits 500g, liquor white sugar 20g, agar 20g, water 1000ml; (3) potato 200g, liquor white sugar 20g, agar 20g, peptone 0.5g, beef extract 0.5g, water 1000ml; Then all batchings are placed in the general pot and dissolve together, put into test tube after dissolving, when putting into test tube, make the liquid inclined-plane reach the 75%-85% of whole test tube, original seed can better be inoculated with medium slant;
Secondly, utilize the steam pressure cooker to carry out high-temperature sterilization, air pressure reaches exhaust in 0.7 o'clock once, is depressed into barometric 1.5mpa then and keeps 120 minutes with final vacuum;
At last, take out test tube after the exhaust, put inclined-plane to cooling back and form the inclined-plane naturally;
C. two, the inoculation of three-class strain, at first,, be placed in order in the nontoxic inoculating hood ready medium or female the kind, sterilize again with potassium permanganate, formaldehyde then, sterilization goes to inoculate after afterwards etc. the sterilization smell drains.At first open ready culture base-material bottleneck during inoculation;
Secondly, open female test tube mouth of planting, get hyphal development is good in its test tube mother and plant the female base in invisible spectro inclined-plane and inoculate, cultivate behind the sealing secondary medium bottleneck with secondary medium;
At last, the medium of second class inoculum and three-class strain is put into nontoxic disinfection cabinet, carry out disinfection with potassium permanganate and formaldehyde equally, smell to be sterilized is inoculated after disappearing again, at first open all bottle caps of three-class strain medium during inoculation, and then open the second class inoculum bottle cap; The bottle that utilizes transfer needle or inoculation spoon that the secondary vaccine is put into the three-class strain medium hits exactly, and each bottle is as the criterion by 3 millimeters, seals the three-class strain bottle then, carries out normal temperature again and cultivates.
Wherein, during described steam autoclave up, temperature is 200 ℃; Before original seed decomposed in the described a step, the original seed of two bases that cut all took out original seed with 0.1% mercury water logging bubble and washes with cold water after 3-5 minute in sterile board, use 75% alcohol wipe again, and then decomposed with female and plant cultivation.
The invention has the beneficial effects as follows: overcome shortcoming of the prior art, increased product yield and improved its quality.
Embodiment
The domesticating cultivation method of a kind of wild bacterium, carry out according to following steps:
A. original seed decomposes, and selects to physically well develop, maturity is that eight to ninety percent original seed decomposes, and the original seed after the decomposition is put into pre-prepd medium in vitro, observes and choose to grow preferably original seed and inoculate with mother culture media;
B. the preparation of mother culture media, at first, (1) the moyashi 500g for preparing to prepare burden in three minutes, liquor white sugar 20g, agar 20g, hickory chick footing soil 50g, water 1000ml; (2) wooden oak bits 500g, liquor white sugar 20g, agar 20g, water 1000ml; (3) potato 200g, liquor white sugar 20g, agar 20g, peptone 0.5g, beef extract 0.5g, water 1000ml; Then all batchings are placed in the general pot and dissolve together, put into test tube after dissolving, when putting into test tube, make the liquid inclined-plane reach the 75%-85% of whole test tube, original seed can better be inoculated with medium slant;
Secondly, utilize the steam pressure cooker to carry out high-temperature sterilization, air pressure reaches exhaust in 0.7 o'clock once, is depressed into barometric 1.5mpa then and keeps 120 minutes with final vacuum;
At last, take out test tube after the exhaust, put inclined-plane to cooling back and form the inclined-plane naturally;
C. two, the inoculation of three-class strain, at first,, be placed in order in the nontoxic inoculating hood ready medium or female the kind, sterilize again with potassium permanganate, formaldehyde then, sterilization goes to inoculate after afterwards etc. the sterilization smell drains.At first open ready culture base-material bottleneck during inoculation;
Secondly, open female test tube mouth of planting, get hyphal development is good in its test tube mother and plant the female base in invisible spectro inclined-plane and inoculate, cultivate behind the sealing secondary medium bottleneck with secondary medium;
At last, the medium of second class inoculum and three-class strain is put into nontoxic disinfection cabinet, carry out disinfection with potassium permanganate and formaldehyde equally, smell to be sterilized is inoculated after disappearing again, at first open all bottle caps of three-class strain medium during inoculation, and then open the second class inoculum bottle cap; The bottle that utilizes transfer needle or inoculation spoon that the secondary vaccine is put into the three-class strain medium hits exactly, and each bottle is as the criterion by 3 millimeters, seals the three-class strain bottle then, carries out normal temperature again and cultivates.
Wherein, during described steam autoclave up, temperature is 200 ℃; Before original seed decomposed in the described a step, the original seed of two bases that cut all took out original seed with 0.1% mercury water logging bubble and washes with cold water after 3-5 minute in sterile board, use 75% alcohol wipe again, and then decomposed with female and plant cultivation.
Among the step c, two, the system of selection of the medium of three-class strain has sawdust medium, cotton seed hulls medium, horse cow dung culture medium, secondly also have wheat bran, white sugar, quicklime, gypsum, superphosphate all to mix by a certain percentage and allocate by natural pH value.Concrete proportioning value is 5: 3.4: 1.57: 0.01: 0.01: 0.01.After the allotment, bottling utilizes the high-temperature sterilization pot to carry out disinfection.Exhausting cold air once is depressed into barometric 1.5mpa then and keeps taking out as standby with final vacuum in 2 hours during toxin expelling.
Because my ground day and night temperature is bigger, winter the temperature difference bigger we utilized manually to heat and controlled temperature.We adopt the method for high-temperature sterilization in the disinfecting process of medium, with disposable when reaching 200 ℃, maintain the temperature within two hours or utilize autoclave sterilization to make pressure reach 1.5mpa and keep overcoming the medium halfway problem of sterilizing with interior in two hours.
Because the domesticating and cultivating temperature and humidity of wild bacterium is very important, as long as there is good mycelia to send, the bacteria growing moulding is got soon.Therefore note the variation of temperature and humidity.
The temperature and humidity commonly used of table one, several wild bacterium domesticating and cultivatings
Original hase differentiation temperature fruit body development temperature optimum temperature ℃ humidity ℃
℃ ℃
Chinese caterpillar fungus 5-12 7-15 15-18 50-60
Hickory chick 15-24 17-28 20-24 70-90
Matsutake 25-30 25-33 25-30 60-80
Wild bacterium behind table two, the artificial domesticating cultivation and the mineral element content of natural wild bacterium
Project
Sample moisture content % crude protein % calcium mg ng phosphorus % potassium % zinc % crude fat butt %
Wild bacterium 11.68 29.1 785.1 1.20 3.58 105.5 4.28
Domestication bacterium 11.78 28.1 952.4 1.24 3.34 101.0 4.04
Wild bacterium behind table three, the artificial domesticating cultivation and the various amino acid contents of natural wild bacterium
By table two, table three as seen, nutritive value and the natural wild bacterium of the wild bacterium that employing this method is cultivated are basic identical, thereby have solved the problem of wild bacterium artificial domesticating cultivation.Broken the wild in the past bacterium situation of supply the market on a small quantity, increased the output of wild bacterium and reduced its production cost, made more people edible.
Claims (3)
1. the domesticating cultivation method of a wild bacterium, it is characterized in that: this method comprises the steps:
A. original seed decomposes, and selects to physically well develop, maturity is that eight to ninety percent original seed decomposes, and the original seed after the decomposition is put into pre-prepd medium in vitro, observes and choose to grow preferably original seed and inoculate with mother culture media;
B. the preparation of mother culture media, at first, (1) the moyashi 500g for preparing to prepare burden in three minutes, liquor white sugar 20g, agar 20g, hickory chick footing soil 50g, water 1000ml; (2) wooden oak bits 500g, liquor white sugar 20g, agar 20g, water 1000ml; (3) potato 200g, liquor white sugar 20g, agar 20g, peptone 0.5g, beef extract 0.5g, water 1000ml; Then all batchings are placed in the general pot and dissolve together, put into test tube after dissolving, when putting into test tube, make the liquid inclined-plane reach the 75%-85% of whole test tube, original seed can better be inoculated with medium slant;
Secondly, utilize the steam pressure cooker to carry out high-temperature sterilization, air pressure reaches exhaust in 0.7 o'clock once, is depressed into barometric 1.5mpa then and keeps 120 minutes with final vacuum;
At last, take out test tube after the exhaust, put inclined-plane to cooling back and form the inclined-plane naturally;
C. two, the inoculation of three-class strain, at first,, be placed in order in the nontoxic inoculating hood ready medium or female the kind, sterilize again with potassium permanganate, formaldehyde then, sterilization goes to inoculate after afterwards etc. the sterilization smell drains.At first open ready culture base-material bottleneck during inoculation;
Secondly, open female test tube mouth of planting, get hyphal development is good in its test tube mother and plant the female base in invisible spectro inclined-plane and inoculate, cultivate behind the sealing secondary medium bottleneck with secondary medium;
At last, the medium of second class inoculum and three-class strain is put into nontoxic disinfection cabinet, carry out disinfection with potassium permanganate and formaldehyde equally, smell to be sterilized is inoculated after disappearing again, at first open all bottle caps of three-class strain medium during inoculation, and then open the second class inoculum bottle cap; The bottle that utilizes transfer needle or inoculation spoon that the secondary vaccine is put into the three-class strain medium hits exactly, and each bottle is as the criterion by 3 millimeters, seals the three-class strain bottle then, carries out normal temperature again and cultivates.
2. according to the domesticating cultivation method of the described a kind of wild bacterium of claim 1, it is characterized in that: during described steam autoclave up, temperature is 200 ℃.
3. according to the domesticating cultivation method of the described a kind of wild bacterium of claim 1, it is characterized in that: before original seed decomposes in the described a step, the original seed of two bases that cut all takes out original seed with 0.1% mercury water logging bubble and washes with cold water after 3-5 minute in sterile board, use 75% alcohol wipe again, and then decompose and female kind cultivation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102150700A CN101878720A (en) | 2010-07-01 | 2010-07-01 | Method for domesticating and cultivating wild mushroom |
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| CN2010102150700A CN101878720A (en) | 2010-07-01 | 2010-07-01 | Method for domesticating and cultivating wild mushroom |
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| CN101878720A true CN101878720A (en) | 2010-11-10 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN104823698A (en) * | 2015-04-19 | 2015-08-12 | 新疆农业科学院植物保护研究所 | Wild lepiota artificial domestication and cultivation method |
| CN105154342A (en) * | 2015-10-27 | 2015-12-16 | 宋瑞鹏 | Method for cultivating liquid-state morchella strain |
| CN106538241A (en) * | 2016-10-31 | 2017-03-29 | 郝哲 | A kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method |
-
2010
- 2010-07-01 CN CN2010102150700A patent/CN101878720A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103155785B (en) * | 2011-12-09 | 2014-04-16 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN104823698A (en) * | 2015-04-19 | 2015-08-12 | 新疆农业科学院植物保护研究所 | Wild lepiota artificial domestication and cultivation method |
| CN104823698B (en) * | 2015-04-19 | 2017-03-22 | 新疆农业科学院植物保护研究所 | Wild lepiota artificial domestication and cultivation method |
| CN105154342A (en) * | 2015-10-27 | 2015-12-16 | 宋瑞鹏 | Method for cultivating liquid-state morchella strain |
| CN106538241A (en) * | 2016-10-31 | 2017-03-29 | 郝哲 | A kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method |
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Application publication date: 20101110 |