CN101182471B - Highly effective cultivating method for Chinese caterpillar fungus - Google Patents
Highly effective cultivating method for Chinese caterpillar fungus Download PDFInfo
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- CN101182471B CN101182471B CN2007101900642A CN200710190064A CN101182471B CN 101182471 B CN101182471 B CN 101182471B CN 2007101900642 A CN2007101900642 A CN 2007101900642A CN 200710190064 A CN200710190064 A CN 200710190064A CN 101182471 B CN101182471 B CN 101182471B
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- caterpillar fungus
- chinese caterpillar
- cordyceps militaris
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Abstract
The invention discloses a highly effective culture method of Chinese caterpillar fungus. The method is that with the liquid fermentation of Chinese caterpillar fungus strain, a large number of Chinese caterpillar fungus mycelium are cultivated after a few days; the cultivated species of Chinese caterpillar fungus can be produced in a large scale; then the Chinese caterpillar fungus mycelium produced after fermentation are inoculated on silkworm chrysalis or rice medium which is treated by surface sterilization; the further cultivation of the Chinese caterpillar fungus mycelium are processed at a certain appropriate temperature and humidity with necessary ventilation and light management; a large amount of artificial cordyceps militaris which has same form and similar function with the wild Chinese caterpillar fungus is obtained after a few days. The highly effective culture method of Chinese caterpillar fungus of the invention has not only rapidness and effectiveness but also low culture cost. The invention has important significance and popularization and application value for the development of precious Chinese medicine resource, the extension of novel application of .silkworm industry resource and the improvement of additional value.
Description
Technical field
The present invention relates to fungal organism and cultivate field, particularly a kind of high-efficient method for cultivating of Cordyceps militaris (L.) Link..
Background technology
Cordyceps militaris (L.) Link. has improves immunizing power, plurality kinds of health care physiological function such as antitumor, anti-oxidant.The cultural method of Cordyceps militaris (L.) Link. has many reports, as the artificial culturing method (ZL87106987.3) of silkworm chrysalis Cordyceps sinensis; The artificial culture method (ZL97113190.2) of Chinese caterpillar funguses such as Cordyceps militaris (L.) Link.; Silkworm chrysalis Cordyceps sinensis industrialized preparing process (ZL00130381.3) etc.But these silkworm chrysalis Cordyceps sinensis all are to utilize solid spawn to cultivate, promptly at first female kind of solid spawn is inoculated on the solid medium, produce through amplifying production solid spawn cultivar step by step, inoculating on the substratum such as silkworm chrysalis or rice, the suitability for industrialized production difficulty is bigger.
Liquid spawn is solid spawn to be transferred on the liquid nutrient medium form through liquid fermenting; it is a kind of advanced person's of getting up of development in recent years fungus growing technique; it has advantages such as the bacterium speed of sending out is fast, efficient is high, pollution is low; be suitable for industrialization, mass-producing and automatic production, thereby help improving labor productivity and economic benefit.
Summary of the invention
Technical problem is in order to solve the existing deficiency of above-mentioned patented technology, the purpose of this invention is to provide a kind of efficiently, can satisfy the Cordyceps militaris (L.) Link. method of cultivation of suitability for industrialized production needs.
Technical scheme in order to achieve the above object, a kind of high-efficient method for cultivating of Cordyceps militaris (L.) Link. of the present invention, the concrete steps of its method are:
1. the preparation of liquid nutrient medium.N by 1: 2: C ratio, an amount of MgSO
4, KH
2PO
4Deng inorganic substance, through dissolving, adjust pH is made liquid nutrient medium, makes aseptic liquid nutrient medium through the autoclaving supervisor again;
2. Cordyceps militaris spawn is inoculated and fermentation culture.The Cordyceps militaris spawn (as Cordycepsmilitaris Link) of highly pathogenicity is inoculated in the aseptic liquid nutrient medium under gnotobasis, and fermentation culture in fermentor tank is until forming a large amount of mycelium;
3. the cultivation of Cordyceps militaris (L.) Link..The Cordyceps militaris (L.) Link. fungus filament that fermentation back is formed be inoculated in the silkworm chrysalis body of sterilizing or other substratum such as rice on, under Chinese caterpillar fungus growing environments such as suitable temperature, humidity and light, cultivate, until growing Cordyceps sporophore.
4. results of Cordyceps militaris (L.) Link..About 45 days of inoculation back, the Cordyccps-militaris-(L.)-link. Sporophore of turning out begins maturation (the sporophore head grows sporophyte), can carry out the results of Cordyceps militaris (L.) Link..
Beneficial effect beneficial effect of the present invention is presented as following four advantages:
The strain fermentation time short, three tier structure kind (female kind, original seed, the cultivar) time is no more than 15 days, solid spawn then more hundred days;
2. inoculation speed is fast, inoculates more than fast ten times than solid is conventional;
Liquid spawn have pollute low, cell age neat, improve more than 10 times than the cultivation output of solid spawn;
4. Cordyceps militaris (L.) Link. fast growth shortens 1/3rd (promptly 45 days beginning maturations, solid spawn vegetative period about 60 days) than cultivation time of solid spawn.
Embodiment
Concrete preparation process of the present invention is as follows:
1. the preparation of liquid nutrient medium.N (as yeast powder) by 1: 2: C (as glucose) ratio, 0.05%MgSO
4, 0.1%KH
2PO
4, 0.2% peptone and 3-4% tackifier after the dissolving, transfer pH6.5-7.0 to make liquid nutrient medium, make aseptic liquid nutrient medium through the autoclaving supervisor again;
2. Cordyceps militaris spawn is inoculated and fermentation culture.The Cordyceps militaris spawn (as Cordycepsmilitaris Link) of highly pathogenicity is inoculated in the aseptic liquid nutrient medium under gnotobasis, and aerobic fermentation was cultivated 3-5 days in fermentor tank, until forming a large amount of mycelium;
3. the cultivation of Cordyceps militaris (L.) Link..The Cordyceps militaris (L.) Link. fungus filament that fermentation back is formed be inoculated in the silkworm chrysalis body of sterilizing or other substratum such as rice on, in suitable temperature (18-23 ℃), humidity (85-95%), light (astigmatism naturally, illumination every day 14-18 hour) and Chinese caterpillar fungus growing environment such as ventilation cultivate down, until growing Cordyceps sporophore.
4. results of Cordyceps militaris (L.) Link..About 45 days of inoculation back, the Cordyccps-militaris-(L.)-link. Sporophore of turning out begins maturation (the sporophore head grows sporophyte), can carry out the results of Cordyceps militaris (L.) Link..The long 3-5cm of sophisticated Cordyccps-militaris-(L.)-link. Sporophore.
Cordyceps militaris spawn fermentation time of the present invention is short, and three tier structure kind (female kind, original seed, the cultivar) time is no more than 15 days, solid spawn then more hundred days; Inoculation speed is fast and easy and simple to handle, and more than fast ten times, inoculation efficient improves several times than the conventional inoculation of solid; Liquid spawn have pollute low, cell age is neat, output is high; Use the Cordyceps militaris (L.) Link. growth of this method cultivation fast, than the cultivation time shortening 1/3rd (beginning in promptly 45 days is ripe) of solid spawn.
Claims (1)
1. a kind of high-efficient method for cultivating of Cordyceps militaris (L.) Link. is characterized in that, steps of the method are:
The preparation of A, liquid nutrient medium, press 1: 2 N: the C proportioning adds 0.05%MgSO
4, 0.1%KH
2PO
4, 0.2% peptone and 3-4% tackifier, through dissolving, adjust pH is that 6.5-7.0 makes liquid nutrient medium, makes aseptic liquid nutrient medium through the autoclaving program again;
Inoculation of B, Cordyceps militaris spawn and fermentation culture are inoculated into the Cordyceps militaris spawn Cordycepsmilitaris Link of highly pathogenicity in the aseptic liquid nutrient medium under gnotobasis, are placed in the fermentor tank fermentation culture 3-5 days, until forming a large amount of mycelium;
The cultivation of C, Cordyceps militaris (L.) Link., the Cordyceps militaris (L.) Link. fungus filament that fermentation back is formed is inoculated in the silkworm chrysalis body of sterilizing or on the rice medium, and placing temperature is that 18-23 ℃, humidity are 85-95%, and illumination is the nature astigmatism, illumination every day was cultivated under the condition in 14-18 hour, until growing Cordyccps-militaris-(L.)-link. Sporophore;
The results of D, Cordyceps militaris (L.) Link. are inoculated back 45 days, and the Cordyccps-militaris-(L.)-link. Sporophore of turning out begins maturation, and the sporophore head grows sporophyte, can carry out the results of Cordyceps militaris (L.) Link..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101900642A CN101182471B (en) | 2007-11-16 | 2007-11-16 | Highly effective cultivating method for Chinese caterpillar fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101900642A CN101182471B (en) | 2007-11-16 | 2007-11-16 | Highly effective cultivating method for Chinese caterpillar fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101182471A CN101182471A (en) | 2008-05-21 |
| CN101182471B true CN101182471B (en) | 2010-09-08 |
Family
ID=39447896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101900642A Expired - Fee Related CN101182471B (en) | 2007-11-16 | 2007-11-16 | Highly effective cultivating method for Chinese caterpillar fungus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101182471B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283493B (en) * | 2013-06-28 | 2015-04-22 | 广东星河生物科技股份有限公司 | Large-scale cultivation method for Guangdong fruiting bodies of cordyceps military |
| CN103688759A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
| TWI645034B (en) * | 2017-08-17 | 2018-12-21 | 財團法人食品工業發展研究所 | Method for cultivating cordyceps militaris fruiting body |
| CN109220523A (en) * | 2018-09-26 | 2019-01-18 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris high yield cultural method |
| CN114410476A (en) * | 2021-10-29 | 2022-04-29 | 徐州鸿宇农业科技有限公司 | Method for culturing high-yield cordycepin silkworm chrysalis cordyceps by taking dry silkworm chrysalis after silk reeling as host |
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2007
- 2007-11-16 CN CN2007101900642A patent/CN101182471B/en not_active Expired - Fee Related
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| CN101182471A (en) | 2008-05-21 |
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| SE01 | Entry into force of request for substantive examination | ||
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Address after: 212028 Zhenjiang, Dantu Metro Industrial Park Rui East Road, No. 9 Patentee after: Jiangsu University of Science and Technology Address before: 212003 Zhenjiang,, Jiangsu, Jiangsu University of Science and Technology, No. Patentee before: Jiangsu University of Science and Technology |
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| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20080521 Assignee: Jiangsu Kang Bio Engineering stock Co., Ltd Assignor: Jiangsu University of Science and Technology Contract record no.: 2015320000228 Denomination of invention: Highly effective cultivating method for Chinese caterpillar fungus Granted publication date: 20100908 License type: Exclusive License Record date: 20150415 |
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Granted publication date: 20100908 Termination date: 20181116 |