CN103340090B - Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer - Google Patents

Abstract

A method for making mushroom barley cultivars by using white loam as a filter layer, characterized in that: the process steps for making the barley cultivars include: preparing materials, including processing and making barley medium for use and taking a sufficient amount of natural white loam for use Adjust the water content of the white loam to 24-27%, and adjust the pH value to 7.3-7.7; bottle the wheat grain medium according to the conventional method, and then load the white loam so that the loaded white loam is located in the wheat grain medium Above and 4-5cm away from the mouth of the bottle, the thickness of the white loam is 1.5-2cm; inoculate after sterilization; then place the culture bottle in the culture room and cultivate the cultivars according to the conventional method. The invention not only plays the role of filtering miscellaneous bacteria, but also overcomes the phenomenon of miscellaneous bacteria pollution in the production process to the greatest extent, and the yield of finished products can reach 99-100%, and the filtering layer is easy to obtain materials and low in cost.

Description

Method for making mushroom barley cultivar by using white loam as filter layer

The present invention is a divisional application with the title of "Using white loam as a filter layer to make mushrooms and Agaricus blazei strains", the application date is January 09, 2012, and the application number is 201210013247.8.

technical field

The invention relates to a production technology of edible fungus strains, in particular to a method for producing mushroom wheat cultivars by using white loam as a filter layer.

Background technique

As we all know, the key prerequisite for the success of artificial cultivation of edible fungi such as Agaricus blazei is the strain. Only excellent strains can achieve high quality and high yield. It is to purify and rejuvenate from the fruiting body to obtain the mother species (also known as the first-class species); the second stage is to produce the original species, that is, to multiply the mother species to the original species, that is, to use the mycelium of the mother species to inoculate Into the original seed culture medium, expand and breed and cultivate into the original seed (secondary species); the third stage is to produce the cultivated species, it is not enough to have the original seed, the next step is to expand it to the cultivated species in the third stage (Promptly three kinds), cultivated species is exactly the bacterial classification (becoming the bacterial classification that can directly put into production) after utilizing the mycelium of original species to expand once again. Since it is difficult to achieve a true aseptic operation state in the production process of mushrooms and Agaricus blazei (referring to original species or cultivated species), the contamination rate of miscellaneous bacteria reaches 20%-30%, even as high as 50%, in order to reduce Strain pollution rate, increase the yield of strains, and several measures to reduce the contamination rate of edible fungi such as mushrooms and Agaricus blazei are:

One of the measures generally adopts the method of larger inoculation volume. Let the strains that need to be cultivated have a sufficient amount of hyphae. When getting along with the miscellaneous bacteria that may be mixed in, use the co-growth advantage to inhibit the growth of miscellaneous bacteria and achieve the effect of improving the yield of the strains.

The second measure is to pack the fermented material (thickness of about 1cm) in the mouth of the bottle. It is about 4cm away from the mouth of the bottle above the culture medium, and the fermented material (made by special fermentation) with a thickness of 1-2cm is loaded, and then sterilized. Because the fermentation material itself is sterile, after the bacteria are inserted, the mycelia will quickly cover the upper part of the entire bottle while eating the material, and it is easy to form a co-growth advantage to inhibit miscellaneous bacteria. This method can greatly reduce the pollution rate , the disadvantage is that the operation is complicated, time-consuming and labor-intensive.

Although using the above-mentioned measures to reduce the contamination rate of bacterial strains has a good effect of improving the yield of bacterial strains in general, due to the traditional production of original species (secondary species) and cultivated species (third-level species) such as mushrooms and Agaricus blazei In the process of seeding), fermented rice straw or cotton husks or cow dung and other fermented materials that are easy to retain moisture are used as the inoculation block of the mother seed or the original seed to form a mycelium culture substrate after germination, and these mycelium culture substrates are used as guides. Primer (Note: These inoculation block materials that form mycelium culture substrate are often referred to as guide material), which will redirect mycelium from the original inoculation block to the new cultivation substrate to facilitate the formation of co-growth advantages (note : as the material of guide material generally all is the fermented material that some nutrition is rich and has the raw material of good moisturizing ability to make through special fermentation to form co-growth advantage favorably), as can be seen from the above, due to large-scale bacteria The production process of the species, especially in the inoculation process, is difficult to achieve a true aseptic operation state, which has a great negative impact on the common practice of using the co-growth advantage of the strain hyphae to inhibit the growth of miscellaneous bacteria, not only reducing the bacteria The pollution rate is limited and the operation is cumbersome.

In addition, with the development of science, traditional dung grass strains (also known as dung grass cultivars) used for edible fungi such as mushrooms and Agaricus blazei have been on the verge of being eliminated, and the next one is to make mushrooms and Agaricus blazei. As the original species and cultivars for production, wheat grain strains emerged as the times require. Edible fungus such as mushrooms and Agaricus blazei have short production cycle, simple process, consistent bacterial age, strong stress resistance, convenient sowing, and not easy to age. It is more resistant to storage and transportation, so it is more important to use the above-mentioned measures to reduce the pollution rate when making wheat grain strains, but it is also difficult to achieve true aseptic operation in the production process, and often due to guidance The material is rich in nutrients, and the mycelium of the miscellaneous bacteria also eats the material and grows very quickly, thus greatly weakening the original co-growth advantage. The rate is low, which seriously restricts and affects the production efficiency.

Contents of the invention

In view of the advantages of convenient inoculation, small amount of seed used, rapid germination and vigorous growth of mycelia, the advantage of forming a biological group on the material surface in a short period of time, reducing the contamination of miscellaneous bacteria, and being more suitable for large-scale production, the present invention The task of the invention is to provide a kind of method that utilizes white loam as filter layer to make mushroom wheat cultivars, so that not only the effect of filtering miscellaneous bacteria can be played, but also the phenomenon of miscellaneous bacteria pollution in the production process can be overcome to the greatest extent (miscellaneous bacteria are not easy Diffusion culture medium) also makes the age of bacteria consistent, and the material used in this filter layer is easy to obtain, the cost is light, and the effect of reducing the contamination rate of bacteria is good.

The present invention is realized by the following technical scheme, this method of the present invention utilizes white loam as filter layer to make mushroom wheat cultivar, and the method still includes the following process steps: the one is to produce female species (also known as first-class species) , the 2nd, produce (wheat grain) original seed (also known as secondary species), the 3rd, produce (wheat grain) cultivated species (also known as tertiary species, be the bacterial classification that can directly put into production), it is characterized in that: wherein The process steps of the production (wheat grain) cultivar (also known as the third-level species) described above include: material preparation, including processing and making wheat grain medium for subsequent use and (from the mountain) getting enough natural white loam for subsequent use; Adjust to 24-27%, adjust the pH value to 7.3-7.7 (such as about 7.5); bottle the wheat grain medium according to the conventional method (into the culture bottle), and then (from the bottle mouth) into the white loam , the white loam soil that packs is positioned at the top of the wheat grain culture medium and is apart from bottle mouth 4-5cm, and the white loam soil thickness is 1.5-2cm; Inoculate after the sterilization treatment, namely insert original species (also known as secondary species); after that Place the culture bottle connected with the original seed in the cultivation room and follow the conventional method to cultivate the cultivated species. After the cultivated wheat grains are prepared, they can be directly put into production to produce bacteria and fruit.

In the present invention, the processing of the wheat grain culture medium can be made according to the conventional method steps, including: soaking, pre-steaming, airing, mixing and bottling (750 milliliter glass seed bottles can be used for the production of wheat grain seeds), Sterilization (after sterilization, it will become wheat grain culture medium) and other steps for production.

The principle and beneficial effects of the present invention are: use the white loam on the mountain as the filter layer when the mushroom wheat cultivated species is produced, so that the original guide material is changed into a filter layer, which can overcome the phenomenon of bacteria pollution in the production process to the greatest extent (almost No pollution), the finished product rate can reach 99%-100%. The principle is that the white loam does not contain organic matter, which makes it difficult for miscellaneous mycelium to reproduce and grow in this soil, but the white loam has better moisture retention ability, so In the production process of mushroom wheat cultivars, using white loam as the filter layer on the wheat grains can filter out miscellaneous bacteria, but has little effect on the normal growth of fungal hyphae, because white loam contains enough water, the mycelia germinated from the inoculum block can pass through (through) the filter layer to reach the wheat grain medium, and even if there are bacteria spores mixed in, it is difficult to grow in this filter layer formed by white loam, and this The materials used in the filter layer are easy to obtain, light in cost and good in effect.

Detailed ways

The following is a specific embodiment of using the technical solution of the present invention to make mushroom wheat cultivars.

This method of using white loam as a filter layer to make mushroom wheat cultivars of the present invention still includes the following process steps: one is to produce the mother species (also known as the first-class species), and the other is to produce (wheat grain) original seeds (secondary species), the third is production (grain) cultivar (tertiary species, a strain that can be directly put into production), characterized in that: the production (grain) cultivar (also known as third-level species) The technological steps of the species) are: preparing materials, including processing and making wheat grain culture medium and getting enough natural white loam from the mountain for subsequent use; adjusting the water content of the white loam to 24-27%, and the pH value to 7.3-7.7 (if adjusted to 7.5 or so); According to the conventional method, the wheat grain medium is bottled (in the culture bottle), and then (from the bottle mouth) is filled with white loam, so that the packed white loam is located above the wheat grain medium and separated from the The mouth of the bottle is about 4-5cm, and the thickness of white loam is 1.5-2cm; inoculate after sterilization, that is, insert the original species (also known as the second-level species); then place the culture bottle connected with the original species in the cultivation room according to the routine The method is to cultivate the cultivars. After the cultivated wheat grains are ready, they can be directly put into production and used to grow bacteria and produce mushrooms.

Claims (2)

1. a method of utilizing white loam to make filter course making mushroom kernel cultivated species, the method comprises following processing step: the one, produce female kind, the 2nd, and produce wheat original seed, the 3rd, produce wheat cultivated species, it is characterized in that: the processing step of wherein said production wheat cultivated species comprises again: get the raw materials ready, comprise that processing and fabricating wheat medium is for subsequent use and get the natural white loam of q.s from mountain for subsequent use; White loam water content is transferred to 24-27%, and pH value is transferred to 7.3-7.7; By wheat medium bottling, reinstall white loam according to conventional method afterwards from bottle mouth position, the white loam that makes to pack into is positioned at the top of wheat medium and apart from bottleneck 4-5cm, white loam thickness is 1.5-2cm; After sterilization treatment, inoculate, access original seed; Afterwards the blake bottle that connects original seed is positioned over to the cultivation of carrying out cultivated species in culturing room according to conventional method.

2. the white loam of utilization according to claim 1 is made the method for filter course making mushroom kernel cultivated species, it is characterized in that: the processing and fabricating of described wheat medium is according to conventional method step, comprising: after immersion, pre-steaming, airing, spice bottling, sterilizing, become wheat medium.