WO2016024836A1

WO2016024836A1 - Method for mass-producing seed potatoes

Info

Publication number: WO2016024836A1
Application number: PCT/KR2015/008506
Authority: WO
Inventors: 김수만; 김광해
Original assignee: 김수만; 김광해
Priority date: 2014-08-13
Filing date: 2015-08-13
Publication date: 2016-02-18
Also published as: KR101544321B1

Abstract

The present invention relates to a method for mass-producing seed potatoes and, more specifically, to a method wherein, when seed potatoes are produced by cultivating potato stalk plants in a screen greenhouse, the growth of the potato stalk plants is temporarily stopped by controlling at least one of a nutrient solution, moisture, and a temperature, to induce the expression of daughter branches, thereby mass-producing sterile seed potatoes without viral infection. The method for mass-producing seed potatoes according to the present invention comprises the steps of: producing potato stalk plants by collecting growing points of potatoes, followed by sterile culture; and producing seed potatoes by cultivating the potato stalk plants in a screen greenhouse, wherein in the step of producing seed potatoes, the growth of the potato stalk plants is temporarily stopped to induce the expression of daughter branches.

Description

【Specification】

[Name of invention]

Mass production method of seed potatoes

Technical Field

The present invention relates to a mass production method of seed potatoes, and more particularly, in the production of seed potatoes by cultivating potato stem plants in a mansil house, by controlling any one or more of nutrient solution, moisture, and temperature to stop the growth of potato stem plants. The present invention relates to a method of inducing the expression of welfare to produce a large amount of sterile seed potatoes not infected with a virus.

Background Art

Potatoes are the four staple crops widely cultivated in 160 countries around the world, and are the most promising alkaline food crops for the development of the food industry in the 21st century. The crops are the crops with the highest energy and yield per unit area. However, if you use virus-infected seed potatoes, the yield will be reduced by 30-80%, so you must use good seed potatoes without virus infection. Domestic seed potatoes spread around 20-30%, and the global average spread around 10%. The low penetration rate of seed potatoes is due to the lack of technology for producing large amounts of seed potatoes from the first disease-free tissue cultured potato stem plants, and the high production cost.

SUBSTITUTE SHEET RULE 26) RO / KR Because In addition, it takes four to five years to grow and grow disease-free tissue cultured potato stem plants and supply them to farmers as seed potatoes. There are various problems in sanitizing seed potatoes during this period. Existing seed potato production and supply system produces small seed potatoes (basic species) from tissue culture potato stem plants by house top or hydroponic cultivation. It is grown and supplied to farms. Such a system has a disadvantage in that it takes about 4 to 5 years to produce seed potatoes supplied to farms from potato stem plants grown in tissues. The reason for the long time is that it takes much time and expense to produce seed potatoes from the first tissue cultured potato stem plants, and the multiplication process is necessary because the system for producing seed potatoes in large quantities has not yet been established.

[Detailed Description of the Invention]

[Technical problem]

The present invention has been made to solve the problems of the prior art as described above in the cultivation of three potato stem plants in the mansil house to produce seed potatoes to control any one or more of the nutrient solution, moisture and temperature of the potato stem plants It is an object of the present invention to provide a method of inducing the expression of welfare by pausing growth and producing a large amount of sterile seed potatoes not infected with a virus.

SUBSTITUTE SHEET RULE 26 RO / KR In addition, another object of the present invention is to provide a method for producing a large number of seed potatoes, seed seed potatoes, seed potatoes with excellent production capacity of seed seeds. In addition, the present invention by producing more than 10 basic species from the potato stem plant was able to disseminate the seed potato of the second generation to the farmhouse that was able to disseminate seed potatoes to the farmhouse only until the fourth to fifth generation conventionally Another object of the present invention is to provide a method for mass production of seed potatoes, which can shorten the production period of seed potatoes for farmhouse dissemination from two to three years from four to five years. The problem to be solved of the present invention is not limited to those mentioned above, and other problems to be solved that are not mentioned will be clearly understood by those skilled in the art from the following description.

Technical Solution

Mass production method of seed potatoes according to the present invention in order to achieve the above and the object comprises the steps of collecting the growth point of the potato, aseptic culture to produce a potato stem plant; And cultivating the potato stem plant in a mansilla house to produce seed potatoes, wherein the seed potato production step is characterized by inducing the expression of welfare by pausing the growth of the potato stem plant. .

SUBSTITUTE SHEET RULE 26 RO / KR In addition, the method for mass production of seed potatoes according to the present invention lowers the dilution multiple of the anti-scarf nutrient solution to the potato stem plants by lowering the concentration of the thick dilute leaves and then suspends the growth of the potato plants. It is characterized by inducing expression. More specifically, it is preferable to lower the dilution drainage of the anti-scarfing nutrient solution to the potato stem plants by 2 to 5 times the standard dilution drainage and to thicken the concentration of the foliar.

In addition, the mass production method of seed potatoes according to the present invention is characterized in that the supply of moisture to the potato stem plant is stopped for a predetermined period of time to stop the growth of the potato plant to induce the expression of welfare. More specifically, it is preferable to stop the water supply for 3 to 10 days by adjusting the potato stem plant according to the weather conditions.

In addition, the mass production method of seed potato according to the present invention is characterized in that the growth of the potato stem plant is maintained at a predetermined low temperature to stop the growth of the potato plant to induce the expression of welfare (l 으로). More specifically, it is preferable to maintain the cultivation temperature of the potato stem plant at 2 ~ 5 ^° C for 2 to 3 days by 5 to 10 hours a day.

Advantageous Effects KR According to the mass production method of seed potatoes according to the present invention having the above configuration, in the production of seed potatoes by cultivating potato stem plants in the mansil house to control any one or more of nutrient solution, moisture and temperature to temporarily grow the growth of potato stem plants Stopping induces the expression of welfare and provides the effect of producing large quantities of sterile seed potatoes that are not infected with the virus. In addition, according to the mass production method of seed potatoes according to the present invention, it is possible to produce a large amount of seed potatoes, which are also excellent seed production of seed potatoes, seed production capacity is also excellent. In addition, according to the mass production method of seed potatoes according to the present invention, by producing more than 10 basic species from the potato stem plants, it is the upper generation that is 2-3 generations that the seed potatoes could not be distributed to farmhouses until 4-5 generations in the past It is possible to supply seed potatoes to farmers, thereby reducing the production period of farmers' seed seed potatoes from 4 to 5 years to 2-3 years. The effects of the present invention are not limited to those mentioned above, and other effects which are not mentioned will be clearly understood by those skilled in the art from the following description.

[Brief Description of Drawings]

SUBSTITUTE SHEET (RULE 26) RO / KR 1 is a photograph showing the growth of potato stem plants in tissue culture in the mass production method of seed potatoes according to the present invention.

Figure 2 is a photograph showing the appearance of a basic species after a week after planting the potato stem plants in the mansion house in the mass production method of seed potatoes according to the present invention. Figure 3a to 3c is a photograph showing the appearance of the basic species produced by the mass production method of seed potatoes according to the present invention.

Figure 4 is a photograph showing the appearance of the original species produced by the mass production method of seed potatoes according to the present invention.

Figure 5 is a photograph showing the appearance of the seed produced by the mass production method of seed potatoes according to the present invention.

[Form for implementation of invention]

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the following contents. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided to ensure that the disclosed contents are thorough and complete, and that the technical spirit of the present invention can be sufficiently delivered to those skilled in the art. Like numbers refer to like elements throughout. 1 is a photograph showing the growth of potato stem plants cultured in tissue in the method for mass production of seed potatoes according to the present invention, Figure 2 is a mass of seed potatoes according to the present invention

SUBSTITUTE SHEET (RULE 26) RO / KR Potato stem plants are planted in the loss house in the production method is a photograph showing the appearance of the base species after one week, Figure 3a to 3c is a view of the basic species produced by the mass production method of seed potatoes according to the present invention Figure 4 is a picture showing the state of the original species produced by the mass production method of seed potatoes according to the present invention, Figure 5 is a state of the original species produced by the mass production method of seed potatoes according to the present invention The picture shows. The present invention relates to a mass production method of seed potatoes, and in the production of seed potatoes by cultivating potato stem plants in the mansion house, by controlling one or more of the nutrient solution, moisture and temperature increase, the welfare of the potato stem plants by stopping the growth ( It relates to a method for producing seed potato in large quantities by inducing the expression of i). The production stage of seed seed potato is (1) potato stem plant cultivation-> (2) the production of seed seed potato through the cultivation of potato stem plants in the loss house-> (3) production of original species-> (4) Production of; Here, the present invention relates to the production step of the basic seed seed potatoes through the cultivation of the house thread house (2) potato stem plant, the potato stem plant is a plant obtained by culturing a clean room tissue by removing the growth point from the potato. Figure 1 shows the appearance of potato stem plants in the growth.

SUBSTITUTE SHEET RULE 26 RO / KR In the present invention, in the cultivation of the potato stem plants in the loss house, by artificially adjusting any one or more of the nutrient solution, moisture and temperature according to the season or crop situation, the welfare of the stems in the ground by pausing the growth of the potato stem plants Induces expression of (iii). Here, welfare (萄枝) refers to a stem that grows from the main stem to the ground, takes root and grows as a stem on which potatoes run.

1. First, the control of nutrient solution will be described. When potato stem plants are grown in a mans house, they provide nutrients for nitric acid, phosphoric acid and kali. Nitrate accelerates growth and leaves green. Phosphoric acid and kali also make it stronger than growth. As a crop, potatoes refer to stalks in the ground. Inhibit the growth of the stems of the genus, so in order to suppress the excessive growth of the stems in the ground, foliar fertilization (葉面施肥) is used to prevent overgrowth. The nutrient solution used for foliar fertilization is composed of only phosphoric acid and kali, except for nitric acid. The commercially available nutritive nutrient solution in the market is usually diluted and used. A standard dilution factor is defined, and in the present invention, the dilution is lowered to a lower value than the standard dilution factor so that the concentration is increased to prevent potato growth. By suspending the growth of the stem plants until they are almost immediately before the asa, the potato stem plants are kept in the ground for reproduction at this moment.

SUBSTITUTE SHEET RULE 26 RO / KR It is destined to express welfare in enjoyment. If the standard dilution multiple of the nutrient solution for preventing overgrowth is 1,000 times, it can be configured by diluting it to 200 to 500 times by lowering it to 2 to 5 times. If the standard dilution multiple is less than 2 times, the effect of pausing growth cannot be achieved, and if it is more than 5 times, it is preferable to maintain the above range because the potato stem plant dies at all. In addition, foliar fertilization of a high concentration of phosphoric acid / kali nutrient solution for pausing the growth of potato stem plants of the present invention is preferably carried out once every 7 to 10 days. In particular, it is more preferable to at the time of sprouting from the stem above the ground. In addition, the induction of welfare expression by the regulation of the nutrient solution is a hot and humid season, and it is more preferable to apply it in the summer when the man is active.

Example 1

Growing potato enjoyment plants prepared by removing the growth point from potatoes and cultivating clean room tissue in the mansill house, 500 times of commercially available nutritive nutrient solution (diluted with water 1,00 (2) Repeated several times, once every 10 days, Phosphoric acid of the commercially available nutritive nutrient solution used in the present invention.

SUBSTITUTE SHEET RULE 26 RO / KR The content of min and kali components is 16-17 wt% and 16-17 wt%, respectively, and the foliar application per 100 pyeong (per 330 m ² ) is about 5 liters (), and the standard dilution drainage is 1,000 times (to prevent water over 1,000 HI) Nutrient solution).

2. Next, the regulation of moisture will be described. When cultivating potato stem plants in the loss house, the supply of water is suspended to suspend the growth of the potato stem plants until the end of the death period, so that the potato stem plants are well-being in the ground for reproduction at this moment. (甸枝) will be expressed. In addition, it is preferable to stop the water supply for pausing the growth of the potato stem plant of the present invention about 3 to 10 days. If it is less than 3 days can not achieve a pause effect of growth, if it exceeds 10 days potato stem plants will be killed at all, so it is preferable to maintain the above range. In addition, induction of welfare expression by controlling moisture is preferably applied in spring and autumn. This is because it is difficult to achieve a pause of growth even when the water supply is stopped in the summer and the water supply is stopped. In the dry winter, the potato stem plants are adapted to the environment where the water supply is somewhat low.

SUBSTITUTE SHEET RULE 26) RO / KR Example 2

In cultivating potato stem plants prepared by removing the growth points from potatoes and culturing in clean room tissues in the mansilla house, increasing the water supply for 3 to 10 days was repeated many times.

3. Finally, the temperature control will be described. Potato stem plants are maintained at a predetermined low temperature, lower than the normal cultivation temperature of 15 to 25 ^° C, when the cultivation of potato stem plants is carried out in the loss house, until the growth of the potato plants is reached until just before the verb. Welfare 枝 is expressed in the ground stem for instant reproduction. In addition, low temperature cultivation for pausing the growth of the potato stem plant of the present invention is preferably maintained at 2 ~ 5 ^° C for 2 to 3 days by 5 to 10 hours a day. If it exceeds 5 ^° C, it is not possible to achieve the effect of pausing growth, and if it is less than 2 ^° C, it is preferable to maintain the above range because potato stem plants will be verbs at all. In addition, it is desirable to apply welfare expression by temperature control in spring and autumn. Because of the high temperature in summer, it requires a lot of cost and facilities to keep the temperature low.

SUBSTITUTE SHEET (RULE 26) RO / KR This is because potato stem plants are somewhat adapted to the low-environmental environment in cold winters.

Example 3

In cultivation of potato stem plants prepared in clean room tissue culture by removing the growth point from potatoes, maintaining ^at 2-5 ^° C for 2 to 3 days for 5 to 10 hours was repeated several times. .

Example 4

Production of basic species

In the present invention, the potato stem plant culture / growth period is about 20 days, and the potato enjoyment plant is dormant for about a period of time (50 to 100) after about 100 days (protozoa is about 70 days, but only about 120 days is grown). The basic species was produced after the growth period. Figure 2 is a photograph showing a state that a base paper is generated after a week after planting the potato stem plant in the loss house, in the conventional case is only one or two basic species are produced, but the examples of Examples 1 to 3 Basic species according to the mass production method of seed potato according to the invention can be confirmed that more than 10 basic species are generated as shown in Figure 3a to 3c. In fact, by artificially controlling at least one or more of the nutrient solution, moisture and temperature according to the present invention according to seasons or crop conditions, the growth of potato stem plants is suspended to induce the expression of welfare.

SUBSTITUTE SHEET (RULE 26) RO / KR It could have produced ten to as many as thirty basic species.

Example 5

Production of native species

Basic species produced in Examples 1 to 3 were planted about 25 weeks per pyeong after a period of dormancy (45 to 90 days for each variety) for a period of time to produce the raw species through a growth period of about 100 days. The yield per pyeong was about 10-25kg. As shown in FIG. 4, the harvester primitive species extends more than 1 m of welfare stem and produces about 100 tubers, indicating that the bean-like basic species of Examples 1 to 3 have enormous energy.

Example 6

Production of seed

The seed stock of Test Example 2 was planted for about 25 weeks per pyeong after a period of dormancy (45 to 90 days for each variety), and the seed stock was produced after a growth period of about 100 days. As shown in FIG. 5, the seed stock was able to produce a considerable size of a weight of 1,385 g, and a large number of tubers of 1 Kg or more were produced. According to the present invention, the average yield of the seed stock was 8.5 Kg per pyeong, and the yield of the seed stock was able to produce up to 25 Kg. According to the mass production method of seed potatoes according to the present invention through Example 5 and Example 6, it is also very excellent in the production capacity of the original species and seed species of the seed seed potatoes

SUBSTITUTE SHEET (RULE 26) RO / KR You can check it. In addition, according to the mass production method of seed potatoes according to the present invention through Examples 1 to 6, by producing a large amount of seed potato from the potato stem plant, it is not distributed to farmers until 4 to 5 generations over the conventional 4-5 years Compared to the old ones, it can be confirmed that the source or source species, which are two or three generations, can be supplied to farms within two to three years. The present invention described above is merely illustrative, and those skilled in the art will appreciate that various modifications and equivalent other embodiments are possible therefrom. Therefore, it will be understood that the present invention is not limited to the forms mentioned in the above detailed description. Therefore, the true technical protection scope of the present invention should be defined by the technical spirit of the appended claims. It is also to be understood that the present invention includes all modifications, equivalents and substitutes within the spirit of the present invention as defined by the appended claims.

SUBSTITUTE SHEET (RULE 26) RO / KR

Claims

[Range of request]

[Claim 1]

Collecting potato growth points and aseptically culturing the potato stem plants; And cultivating the potato stem plant in a mansilla house to produce seed potatoes.

Mass production method of seed potatoes, characterized in that to stop the growth of the potato stem plant in the seed potato production step to induce the expression of welfare (甸枝).

【Claim ² 】

In U, at least

It is characterized by lowering the dilution rate of the nutrient solution in the potato stem plant to a lower dilution rate than the standard dilution rate to fertilize the foliar so that the growth of the potato plant is suspended to induce the expression of welfare. Mass production of seed potatoes.

[Claim 3]

The method of claim 1

A method for mass production of seed potatoes, characterized in that the supply of moisture to the potato stem plant is stopped for a predetermined period of time to stop the growth of the potato plant to induce the expression of welfare.

SUBSTITUTE SHEET (RULE 26) RO / KR

[Claim 4]

The method of claim 1,

A method for mass production of seed potatoes characterized in that the growth of the potato stem plant is maintained at a predetermined low temperature to stop the growth of the potato plant to induce the expression of welfare (fi).

[Claim 5]

The method of claim 2,

Diluting the dilution factor of the anti-overgrown nutrient solution to the potato stem plant by 2-5 times lower than the standard dilution factor, and fertilize the growth of the potato plant to stop the growth of the potato plant, thereby inducing the expression of welfare (T 枝). Mass production of seed potatoes.

[Claim 6]

According to claim 13

Mass production method of seed potato, characterized in that to stop the supply of water to the potato stem plant for 3 to 10 days to stop the growth of the potato plantation to induce the expression of welfare (葡枝).

[Claim 7]

The method of claim 4,

SUBSTITUTE SHEET (RULE 26) RO / KR Planting temperature of the potato stem plant is maintained at 5 ^° C for 2 to 3 days for 5 to 10 hours a day to pause the growth of the potato plant, the expression of welfare (甸枝) characterized in that Mass production method.

SUBSTITUTE SHEET (RULE 26) RO / KR