CN113287576A - Method for artificial feeding of euseiulus nissei in simulated habitat - Google Patents
Method for artificial feeding of euseiulus nissei in simulated habitat Download PDFInfo
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- CN113287576A CN113287576A CN202110524841.2A CN202110524841A CN113287576A CN 113287576 A CN113287576 A CN 113287576A CN 202110524841 A CN202110524841 A CN 202110524841A CN 113287576 A CN113287576 A CN 113287576A
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- euseiulus
- nissei
- habitat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a method for artificial rearing of euseiulus nissei by simulating a habitat, which comprises the following steps: manufacturing an individual breeding device: the transparent acrylic plate comprises two groups of transparent acrylic plates, wherein a group of through holes are formed in the centers of the acrylic plates at the upper ends of the acrylic plates, a layer of black paper towel is arranged in an interlayer of the two groups of acrylic plates, and a layer of black plastic film is adhered to the black paper towel; feeding and hatching the euseiulus nissei eggs by different nutrient additives, feeding, pairing and oviposition in an individual rearing device after the euseiulus nissei eggs are feathered into female adult mites, and repeating the operations on the eggs; and (4) manufacturing a habitat model culture platform, and creating biological factor conditions influencing the growth and development of euseiulus nissei in the habitat model. According to the method, the growth and development conditions of individuals fed with euseiulus nissei by different nutrient substances are evaluated through a life surface technology, and then population expansion of the euseiulus nissei is carried out through simulation of an ideal habitat, so that population expansion and seed preservation are carried out on the euseiulus nissei effectively by using the method, and a theoretical basis is provided for research and development and production practice of pest control products.
Description
Technical Field
The invention belongs to the technical field of biological control of agricultural diseases and insect pests, and particularly relates to a method for artificial feeding of euseiulus nissei in a simulated habitat.
Background
The euseiulus nissei is an important kind of phytoseiulus persisei, and the euseiulus nissei is often used as a preferred predatory mite kind in biological control due to the characteristics of high generation overlapping property, strong fecundity, long service life of female adult mites, high predatory capacity and the like; can prey on various pest mites such as panonychus citri, tetranychus urticae koch, tetranychus cinnabarinus and the like, can prey on eggs and nymphs of small pests such as aphids, whiteflies and the like, and is an important natural enemy of agricultural and forestry pest mites. As a local species in China, the method has the advantages of strong adaptability, small ecological risk and the like, and has important economic significance; meanwhile, the artificial propagation euseiulus nissei is used for preventing and controlling harmful mites, is a biological prevention and control method which is friendly to the ecological environment, and has wide application prospect in agricultural production, particularly green and organic agricultural product production; and thus has long received attention. At present, the large-scale propagation of the aleyrodids (or artificial feed) cannot be expanded by feeding the aleyrodids, and only natural preys, pollen and the like can be relied on.
Therefore, a method for artificially feeding euseiulus nissei in a simulated wild natural habitat is needed, and on the premise of not breaking ecological balance in the simulated habitat, a stable food source is provided, and mass propagation of the euseiulus nissei is supplied durably and safely. In the propagation expanding period, the growth and development conditions of individuals after different nutrient adding substances are fed to euseiulus nissei are evaluated through a life table technology, and the optimal mixed feed is selected for manufacturing a habitat model feeding table.
Disclosure of Invention
Aiming at the defects and problems in the prior art, the invention aims to provide a method for artificially feeding euseiulus nissei in a simulated habitat, which can quickly expand the population, reduce the experiment cost and is simple and easy to operate.
The invention is realized by the following technical scheme:
a method for artificial rearing of euseiulus nissei by simulated habitat comprises the following steps:
1. manufacturing an individual rearing device of euseiulus nissei:
the individual breeding device comprises two groups of transparent acrylic plates, wherein a group of through holes are formed in the centers of the acrylic plates at the upper ends of the acrylic plates, a layer of black paper towel is arranged in an interlayer of the two groups of acrylic plates, and a layer of black plastic film is adhered to the black paper towel; and (3) putting the subsequent euseiulus nissei into the fresh-keeping film, covering the fresh-keeping film with the fresh-keeping film to prevent the euseiulus nisseyi from escaping, pricking the fresh-keeping film with an insect needle to keep air permeability, and finally clamping the two ends with a clamp.
2. The euseiulus nissei applied to the life table technology grows, develops and breeds:
s21, collecting eggs of the euseiulus nissei laid within 24h for testing, selecting the eggs laid by the euseiulus nissei into a plurality of groups of feeding cells by using a No. 0 writing brush, feeding pollen, pollen + sugar, pollen + yeast as different treatment groups for 1 egg in each feeding cell, creating biological factor conditions influencing the growth and development of the euseiulus nissei in a habitat model in the feeding cells, observing once every 8h, and recording the development progress and survival condition;
s22, feeding the adult female mites after emerging, selecting the male mites to be paired with the adult female mites, selecting the male mites to the feeding device in time if the male mites die, observing the oviposition and survival conditions of the euseiulus nisseiuseiuseiuseiuseye once every 24 hours, and recording the early stage of oviposition, the oviposition time, the latest oviposition time, the daily oviposition amount and the service life of the euseiuseiuseiuseiuseiuseiuseiuseyi until the adult female mites die;
s23, lightly transferring the eggs laid every day to a new feeding chamber by using a No. 0 writing brush, and counting the hatchability of the eggs;
s24, S21 to S23 are repeated 3 times per treatment group.
3. Making a habitat model culture platform, and creating biological factor conditions influencing the growth and development of euseiulus nissei in the habitat model:
s31, using a plastic box as a cultivation platform, paving a layer of black sponge soaked in water in the plastic box, and paving a layer of black absorbent paper with the same area on the black sponge for absorbing water of euseiulus nissei;
s32, spreading the punched black plastic film on black absorbent paper, and enabling the plastic film to be tightly attached to the sponge at the edge part without bubbles or bulges;
s33, placing 5-6 egg-laying clusters stacked by colored cotton silk for female adult mite reproduction;
s34, adding nutrient additives into the black plastic film to serve as ideal nutrient substances artificially supplied;
s35, pricking a citrus plant in the center of the culture platform, picking panonychus citri, and simulating field abiotic influence factors to form a habitat model culture platform;
s36, placing the manufactured habitat model cultivation platform at a temperature of 26 +/-1 ℃, a relative humidity of 80 +/-5%, a light period of L: d ═ 14: 10 (simulating biological influencing factors in a habitat).
Preferably, in the process of breeding euseiulus nissei through growth, development and propagation, the optimal combination of the nutrient additives is camellia pollen + yeast.
Compared with the prior art, the invention has the beneficial effects that:
(1) the manufacturing method of the individual rearing device for euseiulus nissei provided by the invention overcomes the problems of large space occupancy rate, time and labor consumption, high escape rate and the like of the traditional rearing box culture method;
(2) the invention utilizes different nutrient additives to feed euseiulus nissei to obtain the optimal mixture by comparison: the camellia pollen and the yeast can achieve higher egg laying amount and longer egg laying period, and provide theoretical basis for biological control of agricultural pests.
(3) The neoseiulus nisseiulus habitat model provided by the invention adopts a method of mixing optimal nutrient additives with camellia pollen and feeding the mixture in combination with natural preys to obtain the condition of abiotic influence factors of the natural habitat, combines with a phytotron, can be fed in large scale, meets the requirement of rapid population expansion of the neoseiulus nisseiulus, is used for research and development of experimental and biological control effect commodities, and realizes the ultimate goal of applying theories to production practice.
Drawings
FIG. 1 is a schematic diagram of the internal side view of an individual feeding device according to the present invention; in the figure, 1-preservative film, 2-upper acrylic plate, 3-through hole, 4-black plastic film, 5-black paper towel, 6-lower acrylic plate and 7-clamp;
FIG. 2 is a schematic diagram showing the overall structure of the installed individual feeding device of the present invention;
FIG. 3 is a diagram of the status of Amblyseius nissei predatory Panonychus citri in a simulated habitat of the invention, wherein A is Amblyseius nissei and B is Amblyseius citri;
FIG. 4 is a graph of hatchability of Euseiulus nissei eggs with different nutrient supplements.
Detailed Description
The invention is further described below with reference to the accompanying drawings and examples. The experimental methods in the following examples are conventional methods unless otherwise indicated, and materials to which the present invention relates are all commonly available and commercially available.
The method for artificial rearing of euseiulus nissei by simulating the habitat comprises the following steps:
step 1, manufacturing of individual feeding device applied to life-sheet technology
As shown in figures 1 and 2, the individual incubator is composed of two transparent acrylic plates (an upper acrylic plate 2 with the size of 250mm × 50mm × 4mm and a lower acrylic plate 6 with the size of 50mm × 50mm × 1 mm), a through hole 3 with the diameter of 2cm is arranged in the middle of the upper acrylic plate 2, a black paper towel 5 with the size of 55mm × 55mm is additionally arranged between the bottom layer and the middle layer of the acrylic plate, and a layer of black plastic film 4 is attached to the black paper towel 5.
(1) Eggs laid in 24 hours are respectively collected from a pollen feeding group, a pollen + sugar and a pollen + yeast treatment group for testing, the eggs laid by euseiulus nissei are picked into a feeding chamber by using a No. 0 writing brush, 80 eggs (1 egg in each feeding chamber) are provided in total, the pollen + sugar and the pollen + yeast are respectively fed to serve as different treatment groups, and the development progress and survival condition are observed once every 8 hours and recorded;
(2) after the euseiulus nisseyi is feathered into female adult mites, feeding the adult mites by using the individual rearing device manufactured in the step 1 (in the specific implementation, the euseiulus nisseyi is placed into the individual rearing device, then the adult mites are covered by a preservative film 1 to prevent the adult mites from escaping, 1 hole is pricked in the preservative film by using an insect needle to keep air permeability, and finally two ends of the adult mites are clamped by using a clamp 7), selecting male mites to be paired with the male mites, picking the male mites to the rearing device in time if the male mites die, observing the oviposition and survival conditions of the euseiulus nisseiuseiuseiuseiuseyi once every 24 hours, recording the pre-oviposition and oviposition time, the latest oviposition time, the daily oviposition amount and the service life of the euseiulus nisseiuni until the adult mites die;
(3) we gently transfer the eggs laid daily to a new rearing chamber with a No. 0 brush pen, and count the hatchability of the eggs. The actual number of female mites successfully mating per treatment was about 40;
(4) the above operation was repeated 3 times per treatment group.
Step 3, manufacturing a habitat model culture platform:
(1) laying a layer of black sponge with the size of 20cm multiplied by 20cm and soaked by water in a plastic box with the size of 25cm multiplied by 30cm, and laying a layer of black absorbent paper with the same area for absorbing water by euseiulus nissei;
(2) spreading a black plastic film with a size of 22cm × 22cm and perforated with 0.5cm holes on black absorbent paper, and tightly adhering the plastic film to the sponge at the edge part (no air bubbles or bubbles exist);
(3) placing 5-6 spawning clusters stacked by colored cotton silk for female adult mite propagation;
(4) adding a nutrient feed mixed by camellia pollen and yeast into the black plastic film to serve as an ideal nutrient substance artificially provided;
(5) pricking a citrus plant with the height of 15cm in the center of a culture table, and picking panonychus citri to simulate a field abiotic influence factor;
(6) placing the manufactured habitat model cultivation platform at a temperature of 26 +/-1 ℃, a relative humidity of 80 +/-5%, a light cycle of L: d ═ 14: 10 (biological influencing factors in the simulated habitat), the state diagram of the Amblyseius nissei A predating panonychus citri B in the simulated habitat is shown in figure 3.
Observing the growth, development and propagation conditions of euseiulus nissei under the condition of adding different nutrient additives, wherein the influence conditions are shown in the following tables 1 and 2:
TABLE 1 development of various status of Euseiulus Nile with different nutritional supplements
| Nutrient supplement | Egg/day | Young mite/day | If mite before the day | Nymph/day | Generation/day |
| Pollen powder | 2.01±0.03a | 0.92±0.02a | 0.94±0.02b | 1.09±0.02a | 4.96±0.04b |
| Pollen and sugar | 2.05±0.03a | 1.00±0.03a | 1.03±0.03a | 1.01±0.03b | 5.11±0.05a |
| Pollen + yeast | 1.95±0.02a | 0.95±0.03a | 0.96±0.22b | 0.98±0.20c | 4.83±0.06c |
TABLE 2 Effect of different nutritional supplements on the longevity and oviposition of Euseiulus nissei
The hatchability of euseiulus nissei eggs with different nutrient additives is shown in fig. 4, and the combination of table 1 and table 2 shows that the pollen + yeast feeding can achieve higher egg laying amount and longer egg laying period.
The foregoing merely represents preferred embodiments of the invention, which are described in some detail and detail, and therefore should not be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the present invention, and these are all within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (5)
1. A method for artificial rearing of euseiulus nissei in a simulated habitat, which is characterized by comprising the following steps:
step S1, manufacturing an individual breeding device: the individual breeding device comprises two groups of transparent acrylic plates, wherein a group of through holes are formed in the centers of the acrylic plates at the upper ends of the acrylic plates, a layer of black paper towel is arranged in an interlayer of each of the two groups of acrylic plates, and a layer of black plastic film is adhered to the black paper towel;
step S2, the euseiulus nisseiulus is applied to the life table technology for growth, development and propagation: feeding and hatching the euseiulus nissei eggs by different nutrient additives, feeding, pairing and oviposition in the individual rearing device after the euseiulus nissei eggs are feathered into female adult mites, and repeating the operations on the eggs;
step S3, making a habitat model culture platform: creating biological factor conditions influencing the growth and development of the euseiulus nissei in a habitat model, and using the biological factor conditions to feed the euseiulus nissei.
2. The method for artificial rearing of euseiulus nissei through simulated habitat according to claim 1, wherein the step S2 specifically comprises:
s21, collecting eggs of euseiulus nissei laid within 24h for testing, selecting the eggs laid by the euseiulus nissei into a plurality of groups of feeding cells by using a No. 0 writing brush, feeding pollen, pollen + sugar and pollen + yeast into each feeding cell by 1 egg, and taking the pollen, the pollen + sugar and the pollen + yeast as different treatment groups, observing once every 8h and recording the development progress and survival condition;
s22, after emerging into female adult mites, feeding the adult mites by using an individual rearing device, selecting male mites to be paired with the adult mites, if the male mites die, selecting the male mites to the rearing device in time, observing the oviposition and survival conditions of the euseiulus nisseiuseiuseiusei once every 24h, and recording the oviposition earlier stage and oviposition time, the latest oviposition time, the daily oviposition amount and the service life until the adult mites die;
s23, lightly transferring the eggs laid every day to a new feeding chamber by using a No. 0 writing brush, and counting the hatchability of the eggs;
s24, S21 to S23 are repeated 3 times per treatment group.
3. The method for artificial rearing of euseiulus nissei through simulated habitat according to claim 1, wherein the step S3 specifically comprises:
s31, using a plastic box as a cultivation platform, paving a layer of black sponge soaked in water in the plastic box, and paving a layer of black absorbent paper with the same area on the black sponge for absorbing water of euseiulus nissei;
s32, spreading the punched black plastic film on black absorbent paper, and enabling the plastic film to be tightly attached to the sponge at the edge part without bubbles or bulges;
s33, placing 5-6 egg-laying clusters stacked by colored cotton silk for female adult mite reproduction;
s34, adding nutrient additives into the black plastic film to serve as ideal nutrient substances artificially supplied;
s35, pricking a citrus plant in the center of the culture platform, picking panonychus citri, and simulating field abiotic influence factors to form a habitat model culture platform;
s36, placing the manufactured habitat model cultivation platform at a temperature of 26 +/-1 ℃, a relative humidity of 80 +/-5%, a light period of L: d ═ 14: 10 in a climatic cabinet.
4. The method for artificial rearing of euseiulus nissei using a simulated habitat according to any of claims 1 to 3, wherein: the nutrient additive for feeding euseiulus nissei adopts camellia pollen and yeast.
5. The method for artificial rearing of euseiulus nissei using simulated habitat according to claim 1, wherein the method comprises the following steps: the outside of the individual breeding device is wrapped by a preservative film, holes are punched in the preservative film by using insect needles to keep air permeability, and finally the two ends of the individual breeding device are clamped by clips to keep fixation.
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2021
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Application publication date: 20210824 |