CN107278741A - The method that brown paddy plant hopper is prevented and treated using Harpactor fuscipes - Google Patents

The method that brown paddy plant hopper is prevented and treated using Harpactor fuscipes Download PDF

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Publication number
CN107278741A
CN107278741A CN201710443734.0A CN201710443734A CN107278741A CN 107278741 A CN107278741 A CN 107278741A CN 201710443734 A CN201710443734 A CN 201710443734A CN 107278741 A CN107278741 A CN 107278741A
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age
plant hopper
paddy plant
brown paddy
harpactor fuscipes
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CN107278741B (en
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刘光华
邓海滨
张鑫
甘泳红
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NANXIONG SCIENTIFIC RESEARCH INSTITUTE OF GUANGDONG TOBACCO
Zhongkai University of Agriculture and Engineering
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NANXIONG SCIENTIFIC RESEARCH INSTITUTE OF GUANGDONG TOBACCO
Zhongkai University of Agriculture and Engineering
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention discloses a kind of method that utilization Harpactor fuscipes prevent and treat brown paddy plant hopper, step is:Step one, the brown paddy plant hopper in the target rice field is sampled using five point sampling and identifies its worm age;Step 2;Then utility efficiency sampling method counts the insect density of the brown paddy plant hopper in 2~3 ages in the target rice field;Step 3, then implement preventing and controlling:First the ponding in the target rice field is drained, then single discharges the 4 age nymphs and 5 age nymphs of Harpactor fuscipes, the quantity ratio of 4 age nymphs of the Harpactor fuscipes, 5 age nymphs and the brown paddy plant hopper in 2~3 ages is 2:1.5~2:60.The present invention reduces the usage amount of chemical pesticide on paddy rice, and reduction paddy pesticide residual protects farmland ecological environment, reliable technical guarantee is provided for grain security.

Description

The method that brown paddy plant hopper is prevented and treated using Harpactor fuscipes
Technical field
The present invention relates to the field of biological control of harmful organism, and in particular to one kind prevents and treats brown paddy plant hopper using Harpactor fuscipes Method.
Background technology
Brown paddy plant hopper [Nalaparvata lugens] category Homoptera Delphacidae, also known as brown back rice plant hopper, are the important pests of paddy rice One of.Be distributed on the brown paddy plant hopper world with causing harm, immigration pattern, again state in terms of rampant mechanism, the research of natural enemy function Inside and outside existing many reports.Since nearly half a century, brown back rice plant hopper occurrence scope is wide, area big and breaks out frequency height, often outburst Cause disaster, as important pests on China paddy rice, while causing serious economic loss.Preventing and treating to planthopper at present, still with Rice resistant variety seed selection and chemical agent are combined based on preventing and treating.A large amount of the unreasonable of chemical agent use, and can not only mitigate The generation of plant hopper, and the residues of pesticides of environmental pollution and soil are easily caused, endanger human health.Make full use of the biology of natural enemy Pest controlling mode turns into one of current research emphasis.
Harpactor fuscipes [Harpactor fuscipes (Fabrieius)] are Semiptera Reduviidae insect predators, main It is distributed in the ground such as Guangdong, Guangxi, Yunnan, Hunan, Fujian and Anhui.Nymph had for five ages altogether, and female male imago is by five age nymph plumages Change is formed.
The content of the invention
It is an object of the present invention to provide a kind of method that utilization Harpactor fuscipes prevent and treat brown paddy plant hopper, control Rice Brown flies The harm of lice, and then the usage amount of chemical pesticide on paddy rice is reduced, reduction paddy pesticide residual protects farmland ecological environment, Reliable technical guarantee is provided for grain security.
The present invention solves the technical scheme that is used of the technical problem:A kind of utilization Harpactor fuscipes preventing and treating brown paddy plant hopper Method, step is:
Step one, when target rice field is in tillering stage or heading stage, using five point sampling in the target rice field Brown paddy plant hopper is sampled and identifies its worm age;
Step 2;When identify the brown paddy plant hopper worm age be 2~3 age when, then utility efficiency sampling method is investigated and united Count the insect density of the brown paddy plant hopper in 2~3 ages in the target rice field;
Step 3, when the target rice field is in tillering stage, the insect density of the brown paddy plant hopper in 2~3 ages is up to 1000/hundred It is above section level, or, when the target rice field is in heading stage, the insect density of the brown paddy plant hopper in 2~3 ages up to 500/encyclopaedia with On;Then implement preventing and controlling:First the ponding in the target rice field is drained, then single discharges 4 age nymphs of Harpactor fuscipes With 5 age nymphs, the quantity ratio of 4 age nymphs of the Harpactor fuscipes, 5 age nymphs and the brown paddy plant hopper in 2~3 ages is 2:1.5~2: 60。
Specifically, the investigation and sampling method of the insect density of the brown paddy plant hopper in 2~3 ages is to use parallel on target rice field Jump method takes an investigation, and every 2 sections of investigation investigate 30 sections, retreaded with ceramic whiteware disc brush last layer machine oil and be put into paddy rice middle and lower part altogether, It is quick to pat under rice strain two, then the quantity for the brown paddy plant hopper for looking into the age of meter 2~3 has been held rapidly, statistics conversion obtains the 2 of this target rice field (unit is the insect density of the brown paddy plant hopper in~3 ages:Head/encyclopaedia).
Further, the method for breeding of the Harpactor fuscipes, step is followed successively by:
(1) fresh light green tobacco leaf is placed in the first culture box, its petiole is wrapped up with the rayon balls for being stained with clear water;
(2) the Harpactor fuscipes female adult pest of yellow meal worm advanced age nymph and post-coitum is positioned on the blade of the tobacco leaf, The Harpactor fuscipes female adult pest of the post-coitum is 1 with the quantity ratio of yellow meal worm advanced age nymph:3~5;
(3) the described first culture box is placed in the growth cabinet of 28 ± 2 DEG C of temperature and relative humidity 60 ± 15%, light Secretly compare L:D=14h:10h;
(4) check whether the Harpactor fuscipes female adult pest lays eggs, take out the Harpactor fuscipes female adult pest after spawning;
(5) the pieces of an egg hatching situation of the Harpactor fuscipes is checked, after hatching, the incubates nymph of Harpactor fuscipes is taken Go out, be placed in the second culture box, single head is raised;
(6) yellow meal worm advanced age nymph, incubates nymph and the Huang of the Harpactor fuscipes are put into the described second culture box The quantity ratio of mealworm advanced age nymph is 1:1;
(7) developmental state of the Harpactor fuscipes in the second culture box described in regular check, periodic collection Harpactor fuscipes 3 age nymphs, 4 age nymphs, 5 age nymphs and adult collect, save backup.
Further, the storage temperature of the step (7) is 22~26 DEG C.
Wherein, the first culture box be the basal diameter 12cm, it is high 5cm's and the plastic casing of air-vent is opened on box top; The second culture box be basal diameter 2cm, it is high 1.5cm's and the plastic caddy of air-vent is opened on box top.
Compared with prior art, with following good effect:The present invention innovatively prevents and treats brown paddy plant hopper using Harpactor fuscipes, The harm of Brown Planthopper is controlled, and then reduces the usage amount of chemical pesticide on paddy rice, reduction paddy pesticide residual protects agriculture Field ecological environment, reliable technical guarantee is provided for grain security, with innovative and perspective, is carried out on this basis artificial Breeding and putting into actual production has bright prospects;Moreover, to breed Harpactor fuscipes by using the method for artificial breeding And stored, to be applied in preventing and treating brown paddy plant hopper.
With reference to embodiment, the invention will be further described.
Embodiment
Embodiment 1
The present embodiment 1 is the method for breeding of Harpactor fuscipes, and its step is followed successively by:
(1) in basal diameter 12cm, fresh light green tobacco leaf is placed in high 5cm plastic casing, tobacco leaf can contact plastic casing Periphery, petiole is wrapped up with the rayon balls for being stained with clear water, and the amount of clear water addition is advisable with just overflowing rayon balls, plastic casing Open air-vent in box top;
(2) the Harpactor fuscipes female adult pest of yellow meal worm advanced age nymph and post-coitum is positioned on tobacco leaf, it is described to hand over The quantity ratio of Harpactor fuscipes female adult pest and yellow meal worm advanced age nymph after matching somebody with somebody is 1:3~5;
(3) plastic casing is placed in the growth cabinet of 28 ± 2 DEG C of temperature and relative humidity 60 ± 15%, Light To Dark Ratio L:D =14h:10h;
(4) check whether the Harpactor fuscipes female adult pest lays eggs daily, by the Harpactor fuscipes female adult pest after spawning Take out, can be mated with other male imagos;
(5) the pieces of an egg hatching situation of Harpactor fuscipes is checked daily, and after hatching, the incubates nymph of Harpactor fuscipes is used Banister bruss single head is chosen, and is placed in basal diameter 2cm, in high 1.5cm plastic caddy, air-vent is opened on box top;Single head is raised;
(6) yellow meal worm advanced age nymph, the blusher are put into the plastic caddy of the incubates nymph equipped with Harpactor fuscipes The true stinkbug incubates nymph and the quantity ratio of yellow meal worm advanced age nymph of hunting is 1:1;
(7) developmental state of the Harpactor fuscipes in plastic caddy, and supplement feed are checked daily;Collect once within every 14 days 3 age nymphs, 4 age nymphs, 5 age nymphs and the adult of Harpactor fuscipes, save backup;
(8) 3 age nymphs of Harpactor fuscipes, 4 age nymphs, 5 age nymphs and adult are in 22~26 DEG C of preservations.
Wherein, the first culture box be the basal diameter 12cm, it is high 5cm's and the plastic casing of air-vent is opened on box top;Second Culture box be basal diameter 2cm, it is high 1.5cm's and the plastic caddy of air-vent is opened on box top.
Each worm state survival of the daily observed and recorded Harpactor fuscipes of the present embodiment 1 and developmental state obtain data, are shown in Table 1, with The Harpactor fuscipes well-grown that yellow meal worm advanced age nymph is fed.Each age nymph survival rate of Harpactor fuscipes 83.05% with On.One age, two ages, three ages, four ages, the phase of going through in five ages are respectively 7.71 days, 6.90 days, 8.98 days, 10.05 days, 16.20 days, Nymphal period is about 50 days.It can be seen that, yellow meal worm advanced age nymph makees preferably meet Harpactor fuscipes growth for examination feed Development needs.
Each age nymph survival rate of the Harpactor fuscipes of table 1 and development duration (average value ± standard error)
Those skilled in the art understand that the raising of yellow meal worm is relatively easy, and technology more ripe and cost is relatively low, no By seasonal effect, had a clear superiority compared with prodenia litura and other artificial eye-liquid feeds.
Embodiment 2
The present embodiment 2 is the prey ability experiment of Harpactor fuscipes, and its step is as follows successively:
I. tillering stage paddy rice is planted in diameter 25cm Plastic Drum, enclosing is done using the uniform lucite paper for pricking hole, And prevent test worm from escaping with grenadine and rubber band capping;
II. Plastic Drum is placed in ventilation and the good outdoor environment of illumination, daily mean temperature is 25 ± 2 DEG C;
III. first 3 age nymphs of Harpactor fuscipes, 4 age nymphs, 5 age nymphs and female adult pest are satiated with food 24 hours before testing, Then starvation is tested for 48 hours;
IV. the brown paddy plant hopper in 3 ages of access 30 is raised in the strain of potted plant rice, when it grew to for 4 age, in the Plastic Drum 3 age nymphs, 4 age nymphs, 5 age nymphs and the female adult pest of the Harpactor fuscipes after step III processing are inside delivered respectively.
The present embodiment 2 determines 3 age nymphs, 4 age nymphs, 5 age nymphs and the female adult pest of Harpactor fuscipes to 4 age brown paddy plant hoppers Predation experiment.Per the quantity 30 of the brown paddy plant hopper in the strain of basin rice.The quantity of survival brown paddy plant hopper is checked after 1 day, 3 days, 7 days.Experiment If 5 repetitions.Calculate the corrected mortality of brown paddy plant hopper.
After measure, 1 day after processing, 3 days, the corrected mortality of the brown paddy plant hopper of 7 days be shown in Table 2.As shown in Table 2, blusher is really hunted 4 ages of stinkbug and 5 age nymphs are best to the prey ability of brown paddy plant hopper, and the death rate of brown paddy plant hopper reaches 100% after 7d.3 age nymphs and Female adult pest is poor to the prey ability of brown paddy plant hopper, and the death rate of brown paddy plant hopper is respectively 60.67% and 72.67% after 7d.
Prey ability of the Harpactor fuscipes of the different larval instar of table 2 to brown paddy plant hopper
Embodiment 3
The present embodiment 3 is the method that brown paddy plant hopper is prevented and treated using Harpactor fuscipes, and step is followed successively by:
Step one, when target rice field is in tillering stage or heading stage, using five point sampling in the target rice field Brown paddy plant hopper is sampled and identifies its worm age;
Step 2, when identify the brown paddy plant hopper worm age be 2~3 age when, then utility efficiency sampling method is investigated and united The insect density of the brown paddy plant hopper in 2~3 ages in the target rice field is counted, the target rice field of the present embodiment 3 is 5m2Cell, An investigation, every 2 sections of investigation, the section paddy rice of Gong Cha 30, with ceramic whiteware disc brush last layer machine are taken using parallel jump method on target rice field Oil, which retreads, is put into paddy rice middle and lower part, quick to pat under rice strain two, then has held the quantity for the brown paddy plant hopper for looking into the age of meter 2~3, statistics rapidly Conversion obtains the insect density of the brown paddy plant hopper in 2~3 ages in this target rice field, and (unit is:Head/encyclopaedia).
Step 3, when the target rice field is in tillering stage, and the insect density of the brown paddy plant hopper in 2~3 age reaches It is more than 1000/encyclopaedia, or, when the target rice field is in heading stage, and the insect population of the brown paddy plant hopper in 2~3 age is close Spend up to more than 500/encyclopaedia;Then implement preventing and controlling:First the ponding in the target rice field is drained, then single release blusher The true 4 age nymphs and 5 age nymphs for hunting stinkbug, 4 age nymphs of the Harpactor fuscipes, the number of 5 age nymphs and the brown paddy plant hopper in 2~3 ages Amount is than being 2:1.5~2:60.
Embodiment 4
The present embodiment 4 is the prevention effect experiment for the method that brown paddy plant hopper is prevented and treated using Harpactor fuscipes, and experiment is in triplicate.
The prevention effect experiment of the method for brown paddy plant hopper is prevented and treated using Harpactor fuscipes, step is as follows successively:
I. the experiment rice field plot area that each processing is chosen is 5m2, field is fixed on by wooden frame, size is long 2m × wide 2m × high 1.8m, periphery is surrounded with the grenadine of 20 mesh or so, and the wicket of a free switch, convenient turnover, per small are left in side Interval is every more than 100cm;Experiment sets 3 repetitions;
Ii. it will raise to the Harpactor fuscipes of corresponding age to choose and be put into plastic caddy, be satiated with food 24 hours, it is then hungry Discharged within 48 hours;
Iii. the insect population for the brown paddy plant hopper for clapping method investigation rice 2~3 ages of clump before stinkbug using basin is put;
Iv. 3 ages, 4 ages, 5 age Harpactor fuscipes nymphs or each 15 of female adult pest are respectively connected in different districts, during release The cloudy calm condition of selection, Harpactor fuscipes are placed in and shrouded in rice clump at from 10~15cm of earth's surface;
V. put after stinkbug 1 day and 7 days, sampled by investigation method same step iii, the residual work borer population of investigation brown paddy plant hopper, Statistics encyclopaedia residual individuals simultaneously calculate correcting controling effect.
The field control effect for putting 1 day and 7 days after stinkbug is shown in Table 3.
Field efficacy of the Harpactor fuscipes in each worm age of table 3 to brown paddy plant hopper
As shown in Table 3, release the age of Harpactor fuscipes 4,5 age nymphs preventing and treating brown paddy plant hopper effect preferably, prevention effect is More than 90%, significantly larger than 3 age nymphs and adult controlling effect, difference 55.67% and 66.79%.Can with reference to the result of table 1 , 4 ages of Harpactor fuscipes, the optimal age that 5 age nymphs are predation brown paddy plant hopper.
In summary, in practice, according to relevant documentation & info, when temperature is 26 DEG C, the age of brown paddy plant hopper 2,3 ages go through the phase About 2 day time, and 4 ages of Harpactor fuscipes go through the phase about 10 days, the phase of going through in 5 ages is about 18 days, sees supplementary notes.Can See, after 4 ages, 5 age nymphs in the age of brown paddy plant hopper 2~3 release Harpactor fuscipes, the age of brown paddy plant hopper will grow into advanced age nymph (4 ages or 5 ages).That is, discharging Harpactor fuscipes at the age of brown paddy plant hopper 2,3 age, its main prey is that brown paddy plant hopper is high Age nymph (4 ages or 5 ages), rather than low age nymph (2 ages or 3 ages).Similarly, in the age of brown paddy plant hopper 4 (phase of going through is about 3 days), 5 Discharge Harpactor fuscipes during age (phase of going through is about 4 days), the worm state of Harpactor fuscipes predation brown paddy plant hopper be likely to brown paddy plant hopper into Worm, this is unfavorable for Harpactor fuscipes predation.It can be seen that, Harpactor fuscipes are discharged at 2~3 age of brown paddy plant hopper, can obtain and preferably catch Eat effect.
In addition, being calculated by above-mentioned predation data, the age of Harpactor fuscipes 4, the number of 5 age nymphs and 2~3 age brown paddy plant hopper nymphs Amount is than being 2:1.5~2:When 60, on the premise of rationally control cost, preferable control action can be played to brown paddy plant hopper.
Embodiment 5
The present embodiment 5 is test method, to drain the brown paddy plant hopper prevention effect of the ponding to Harpactor fuscipes in target rice field Influence.
The consistent rice field field of rice varieties, breeding time, growing way is selected as system investigation and tests field, release blusher is true Hunt by Rice Cropping standard progress Routine Management before stinkbug, every the insect density of ten days follow-up investigation rice field brown paddy plant hoppers.Investigation side Method is sampled using parallel great-jump-forward, every 2 section paddy rice of investigation, and 30 section paddy rice are investigated altogether.Basin claps method, and disk lightly inclines when looking into worm Oblique cutting enters rice row, and disk lower edge is close to native face or water surface rice clump base portion, and quick to flop plant middle and lower part, continuous shooting is twice.Record respectively The adult quantity of the brown paddy plant hopper of different wing and sex and each age nymph quantity, and it is converted into encyclopaedia worm amount (head/encyclopaedia). Selection 5m in selected system investigation experiment field2Cell, drains water and does not drain two kinds of water (depth of water is about 2~3cm) respectively Processing, cell periphery is surrounded with 2 meters high 20 mesh gauze, when the quantity of brown paddy plant hopper reaches certain density (May 8), in cell 10 Harpactor fuscipes 4 age nymphs, the quantity of periodic investigation brown paddy plant hopper are inside discharged respectively.Two cell planthopper variable densities are obtained Table 4 is obtained, is analyzed.
The Population of Rice Brown Planthopper Number dynamics of table 4 (head/encyclopaedia)
As shown in Table 4, population number of the population number well below not drainage sunk well of rice terrace is drained.As a result show, Carrying out drainage sunk well is conducive to Harpactor fuscipes to prey on brown paddy plant hopper.
It is below supplementary notes:
1st, worm age
Worm age is a kind of biological vocabulary, under normal circumstances, and the growth of insect children phase will peel to a certain extent, insect Age or growth course can make index with number of times is casted off a skin.The larva just come out to from egg hatching before peeling for the first time is referred to as First instar larvae, the larva after being peeled through first time is referred to as the second instar larvae.The rest may be inferred by analogy.
2nd, the worm age of Harpactor fuscipes
Harpactor fuscipes nymph has five ages, that is, one has five ages, after casting off a skin for the last time, that is, changes For adult, male and female at this moment can be differentiated.By observational study, (body length, body are wide, head capsule length, head for the growth indexes of Harpactor fuscipes Shell is wide, body weight and development duration are shown in Table 5 and table 6.
Each worm state growth indexes measured value of the Harpactor fuscipes of table 5 (average value ± standard error)
Development duration (average value ± standard error) (unit of the Harpactor fuscipes of table 6:My god)
3rd, yellow meal worm and its advanced age nymph.
Yellow meal worm [Tenebrio molitor] is called bread worm, and coleoptera is under the jurisdiction of on entomological taxonomy, intends walking First section, meal beetle Eimeria (Tenebrio).The nymph body of yellow meal worm is elongated, cylindrical, and mature larva body length is about 24~ 29mm, newly hatched larvae is milky, after be changed into yellowish-brown, each section back side front and rear edge is filbert, and internode and the outside of belly are yellow-white, respectively Instar larvae body length and head capsule it is wide be distinguishing larval instars Main Basiss.Yellow meal worm was generally for 12 ages, and advanced age described in this patent, nymph was The nymph in 8-12 ages.
4th, section:The technical term of paddy rice, is typically made up of 5~20 individual plants, and some usual documents are " clump ".
5th, parallel jump method:For the national sampling survey commonly used in Entomological Ecology.Taken in rice field every some rows A line (or several rows) is investigated.The investigation quantity of rice strain can conveniently be set according to paddy environment, experiment demand or experiment.
6th, correcting controling effect and corrected mortality calculation formula are:
Correcting controling effect=(treatment region plant hopper decline rate-check plot plant hopper decline rate)/(1- check plots plant hopper is declined Rate) × 100%;
Corrected mortality=[(the processing death rate-control death rate)/(1- compares the death rate)] × 100%.
The invention is not limited in above-mentioned embodiment, if the various changes or modification to the present invention do not depart from the present invention Spirit and scope, if these are changed and modification belongs within the scope of the claim and equivalent technologies of the present invention, then this hair It is bright to be also intended to comprising these changes and modification.

Claims (5)

1. a kind of method that utilization Harpactor fuscipes prevent and treat brown paddy plant hopper, it is characterised in that step is:
Step one, when target rice field is in tillering stage or heading stage, using five point sampling to brown winged in the target rice field Lice is sampled and identifies its worm age;
Step 2, when identify the brown paddy plant hopper worm age be 2~3 age when, then utility efficiency sampling method is investigated and counts institute State the insect density of the brown paddy plant hopper in 2~3 ages in target rice field;
Step 3, when the target rice field is in tillering stage, the insect density of the brown paddy plant hopper in 2~3 ages up to 1000/encyclopaedia with On, or, when the target rice field is in heading stage, the insect density of the brown paddy plant hopper in 2~3 ages is up to more than 500/encyclopaedia; Then implement preventing and controlling:First the ponding in the target rice field is drained, then single discharges the 4 age nymphs and 5 of Harpactor fuscipes The quantity ratio of age nymph, 4 age nymphs of the Harpactor fuscipes, 5 age nymphs and the brown paddy plant hopper in 2~3 ages is 2:1.5~2:60.
2. the method for brown paddy plant hopper is prevented and treated using Harpactor fuscipes as claimed in claim 1, it is characterised in that the Harpactor fuscipes Method for breeding, step is followed successively by:
(1) fresh light green tobacco leaf is placed in the first culture box, its petiole is wrapped up with the rayon balls for being stained with clear water;
(2) the Harpactor fuscipes female adult pest of yellow meal worm advanced age nymph and post-coitum is positioned on the blade of the tobacco leaf, it is described The Harpactor fuscipes female adult pest of post-coitum is 1 with the quantity ratio of yellow meal worm advanced age nymph:3~5;
(3) the described first culture box is placed in the growth cabinet of 28 ± 2 DEG C of temperature and relative humidity 60 ± 15%, Light To Dark Ratio L:D=14h:10h;
(4) check whether the Harpactor fuscipes female adult pest lays eggs, take out the Harpactor fuscipes female adult pest after spawning;
(5) the pieces of an egg hatching situation of the Harpactor fuscipes is checked, after hatching, the incubates nymph of Harpactor fuscipes is taken out, It is placed in the second culture box, single head is raised;
(6) yellow meal worm advanced age nymph, the incubates nymph of the Harpactor fuscipes and yellow meal worm are put into the described second culture box The quantity ratio of advanced age nymph is 1:1;
(7) developmental state of the Harpactor fuscipes in the second culture box described in regular check, 3 ages of periodic collection Harpactor fuscipes Nymph, 4 age nymphs, 5 age nymphs and adult collect, and save backup.
3. the method for brown paddy plant hopper is prevented and treated using Harpactor fuscipes as claimed in claim 1, it is characterised in that:The insect density Investigation and sampling method is to take an investigation using parallel jump method on target rice field.
4. the method for brown paddy plant hopper is prevented and treated using Harpactor fuscipes as claimed in claim 3, it is characterised in that:The parallel jump method For every 2 sections of investigation investigate 30 sections, retreaded with ceramic whiteware disc brush last layer machine oil and be put into paddy rice middle and lower part altogether, quick to pat rice strain Under two, then the quantity for the brown paddy plant hopper for looking into the age of meter 2~3 has been held rapidly, statistics conversion obtains the brown of 2~3 ages in this target rice field and flown The insect density of lice.
5. the method for brown paddy plant hopper is prevented and treated using Harpactor fuscipes as claimed in claim 2, it is characterised in that:The step (7) The temperature of preservation is 22~26 DEG C.
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CN109644946A (en) * 2018-12-29 2019-04-19 广州瑞丰生物科技有限公司 A kind of method for releasing of Predacious function
CN112674034A (en) * 2020-12-24 2021-04-20 广东省烟草南雄科学研究所 Method for feeding red-spotted bugs in large scale by utilizing endive super-oncozus and application

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Publication number Priority date Publication date Assignee Title
CN108513953A (en) * 2018-04-09 2018-09-11 云南农业大学 A method of parasitic wasp is improved to hidden kind of parasitic rate of Bemisia tabaci MED
CN109644946A (en) * 2018-12-29 2019-04-19 广州瑞丰生物科技有限公司 A kind of method for releasing of Predacious function
CN109644946B (en) * 2018-12-29 2021-07-16 广州瑞丰生物科技有限公司 Release method of fleahopper furgus
CN109644950A (en) * 2019-02-11 2019-04-19 广东省烟草南雄科学研究所 A kind of Harpactor fuscipes large-scale breeding method
CN109644950B (en) * 2019-02-11 2021-11-26 广东省烟草南雄科学研究所 Large-scale breeding method for red-color lygus lucorum
CN112674034A (en) * 2020-12-24 2021-04-20 广东省烟草南雄科学研究所 Method for feeding red-spotted bugs in large scale by utilizing endive super-oncozus and application

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