CN102487967A - Preparation method of biopesticide - Google Patents
Preparation method of biopesticide Download PDFInfo
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- CN102487967A CN102487967A CN2011103602413A CN201110360241A CN102487967A CN 102487967 A CN102487967 A CN 102487967A CN 2011103602413 A CN2011103602413 A CN 2011103602413A CN 201110360241 A CN201110360241 A CN 201110360241A CN 102487967 A CN102487967 A CN 102487967A
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Abstract
The invention belongs to the technical field of chemical inventions, and discloses a preparation method of a biopesticide. The preparation method of the biopesticide utilizes a raw material of foots obtained by barbadosnut biodiesel production to realize preparation of the biopesticide. The preparation method of the biopesticide comprises the following steps of 1, adding 20 to 60% of a potassium hydroxide solution or a sodium hydroxide solution into foots obtained by barbadosnut biodiesel production at a temperature of 40 to 100 DEG C, adjusting a pH value until the pH value is 12, and preparing emulsion, or 2, dispersing foots obtained by barbadosnut biodiesel production by a dispersant, an emulsifier, an antifreezing agent and a stabilizing agent to obtain aqueous emulsion, wherein the emulsion or the aqueous emulsion is the biopesticide. The biopesticide obtained by the preparation method provided by the invention has good effects of killing crop pests of pomacea canaliculata, pieris rapae L. and the like, has a low cost and good effects, and produces low pollution on the environment.
Description
Technical field
The invention belongs to chemistry invention technical field, be specifically related to a kind of Jatropha curcas oil that comes from and produce oil foot after the biodiesel, be applied to kill plant insect as agricultural chemicals.
Technical background
As everyone knows, with chemical pesticide relatively, plant pesticide has environmental friendliness,, advantage such as be difficult for develop immunity to drugs low to toxicity to man and livestock, is the focus of pesticide research and application.
Jatropha curcas is a kind of impoverishment tolerant arid, adaptable plant, and its kind benevolence oil content is high, is the desirable feedstock of biodiesel, is considered to the most rising a kind of energy-source plant.Exploitation has had a large amount of research as biodiesel about Jatropha curcas, and while toxic component wherein also obtains paying close attention to as the potential value of natural insecticide.Reported the insecticidal activity [GUIHAIA, 2011,31 (1): 129] of little seeds of a tung oil tree shell extract like people such as Li Yuchuan, people such as Wang Zhaoyu have studied drug effect [Nanfang Medical Univ journal, 2009 of little tungoiltree juvenile leaf methanolic extract to Pomacea canaliculata; 29 (6) 1235-1237], Li Jings etc. have reported that the Jatropha curcas terpene alcohol I that from seed, extracts is as the application of biopesticide (Chinese patent number: 200610020278.0).
Pomacea canaliculata (Pomacea canaliculata Lamarck) belongs to Mollusca, Gastropoda, and Mesogastropoda, Ampullariidae originates in South America Amazon River basin.Introduced China as winkle in 1981, because of its adaptability is strong, breeding becomes the huge exotic invasive species of harm rapidly, and to crops, especially paddy rice is very harmful, can cause the aquatic crops underproduction such as paddy rice more than 50% when serious, even total crop failure.Simultaneously, breechblock can also be propagated the Guangdong Guan Yuan nematode that causes human eosinophilic meningoencephalitis.
Cabbage caterpillar (Pieris rapae L) claim imported cabbageworm again, belongs to the Lepidoptera Sulfur butterfly.Distribution is almost all arranged all over the world, all have in China general each province that distributes.Brassicaceous vegetables such as this worm main harm wild cabbage, cauliflower, Chinese cabbage, radish, rape are had a liking for thick leafy vegetable partially, and traditional Chinese medicines such as Radix Isatidis are also vulnerable.Its larva stings food host blade, only gnaws mesophyll before 2 ages, stays the layer of transparent epidermis; Nibble the blade hole after 3 ages or incise, blade is all eaten up only residual thick vein and petiole when serious; Cause total crop failure, the ight soil of cabbage caterpillar discharge simultaneously infects soft rot again easily.
The biopesticide that derives from plant is usually relatively more expensive, is difficult to the universal low agricultural product of added value that are used for and produces.Biopesticide with the preparation of Jatropha curcas oil foot all has good killing effect to above-mentioned two kinds of insects.And the Jatropha curcas oil foot does not almost have cost as a kind of discarded object, and the technical process that is processed as agricultural chemicals is also fairly simple, can be used as a kind of cheap biopesticide and uses.
Summary of the invention
Characteristic of the present invention is that the discarded object oil foot after the Jatropha curcas production biodiesel is used as biopesticide.The utilization of this oil foot has not only solved the processing of discarded object, also can be used as cheap it and effective biopesticide.
Jatropha curcas oil has insecticidal activity, and handling its structure of back through strong acid and strong base possibly change, and does not detect several kinds of diterpene that insecticidal activity is arranged that document is reported in the oil foot.Experiment proof water soluble ingredient activity is very low, and fat-soluble part insecticidal activity still has activity preferably.Because oil foot remains grease, be insoluble in water, effective kill pests is so need be dispersed in the water.Owing to contain a large amount of nonesterified LCFAs in the oil foot, can be used as surfactant and be beneficial to the liposoluble constituent with insecticidal activity is scattered in the water so add soap that highly basic forms.Other can also can be used for the dispersion with insecticide with the emulsifier that liposoluble constituent is dispersed in the water.
The present invention utilizes oil foot after the Jatropha curcas production biodiesel at 40-100 ℃ of potassium hydroxide or sodium hydroxide solution that adds 20-60%, regulates the pH value and is 12 and be prepared as emulsion; Or oil foot disperseed with emulsifier, dispersant, antifreezing agent, stabilizing agent and the aqueous emulsion made; This aqueous emulsion is biopesticide.
According to the common preparation method of the aqueous emulsion of agricultural chemicals, selected emulsifier is an APES, like styryl phenol polyethenoxy ether, methyl-benzyl APEO, also can select alkylphosphonic etc. for use; Dispersant is sodium phosphate trimer and sodium methylene bis-naphthalene sulfonate; Antifreezing agent is a propane diols; Stabilizing agent is a phenyl glycidyl ether; Dispersion medium is a water.
Embodiment
Through embodiment the present invention is specifically described below.
Embodiment 1: the oil foot sample 55g that gets the biodiesel of methyl alcohol/sulfuric acid/Jatropha curcas grease exchange preparation adds in the 500ml round-bottomed flask and is heated to 80 ℃, and stirring condition drips 45g 20% potassium hydroxide solution down, continues to stir 30 minutes, and preparation becomes emulsion.
Embodiment 2: the oil foot sample 55g that gets the biodiesel of methyl alcohol/sulfuric acid/Jatropha curcas grease exchange preparation adds in the 500ml round-bottomed flask and is heated to 80 ℃; Stirring condition drips 15g 60% potassium hydroxide solution down; Continue to stir 30 minutes, adding water 30g preparation becomes emulsion.
Embodiment 3: the oil foot sample 55g that gets the biodiesel of methyl alcohol/sulfuric acid/Jatropha curcas grease exchange preparation; Styryl phenol polyethenoxy ether 10g, sodium phosphate trimer 6g, phenyl glycidyl ether 6g, propane diols 3g and water 20g are added in the high speed shear mixing emulsifier successively, and adopting high speed shear to be mixed with becomes moisture 20% barbadosnut seed oil foot emulsion.
Embodiment 4: the infusion method among the WHO " the whole screen method in molluscicide laboratory " is adopted in Pomacea canaliculata poisoning experiment.Two kinds of barbadosnut seed oil foot emulsions are mixed with solution in different concentration respectively, place the glass container of capacity 1L, throw in 10 healthy Pomacea canaliculatas for every group; Gauze is added a cover in fully submergence on container, take out respectively in 24,48, during 72h; With dechlorination water flushing 3 times, in the condition of raising held 72h, inspection then: opening the operculum scrambler is live body; Remainder breaks the spiral shell shell into pieces and with dissecting its software of acupuncture, the nonresponder is a dead volume.20mg.L is set simultaneously
-1The methaldehyde and blank control group as contrast, 3 repetitions are established in each processing.20～25 ℃ of test temperatures.The result sees table one.
Table one kills the Pomacea canaliculata experimental result
Embodiment 5: dipping leaf dish feeding method is adopted in cabbage caterpillar poisoning experiment.Fresh cabbage leaves cleaned dry, break into the leaf dish of 2cm * 2cm with square card punch, the Jatropha curcas oil foot emulsion dilution of putting into variable concentrations respectively floods 5s; After taking-up is dried, put into diameter and be the preserve moisture culture dish of filter paper of being lined with of 9cm, every ware is put 5 and is handled the leaf dish; Insert cabbage caterpillar 3 instar larvaes of hungry 3h; 1 in every ware, 10 of every processing are carried out 3 times and are repeated; Simultaneously with the negative contrast of acetone soln after the dilution of 0.1% lauryl sodium sulfate aqueous solution, with 50% phoxim emulsifiable concentrate as positive control.Be to raise in 60%～70% the biochemical incubator for (25 ± 1) ℃, relative moisture supplying the examination larva to be put in temperature then, detect the cabbage caterpillar lethality respectively at 12,24,48, behind the 72h, experimental result is seen table two.
Table two poisoning cabbage caterpillar experimental result
Claims (3)
1. the preparation method of a biopesticide is characterized in that: the oil foot after the Jatropha curcas production biodiesel is at 40-100 ℃ of potassium hydroxide or sodium hydroxide solution that adds 20-60%, regulates the pH value and is 12 and be prepared as emulsion; Or oil foot disperseed with emulsifier, dispersant, antifreezing agent, stabilizing agent and the aqueous emulsion made; This aqueous emulsion is biopesticide.
2. according to the preparation method of the biopesticide described in the claim 1, it is characterized in that: described emulsifier is APES or alkylphosphonic or styryl phenol polyethenoxy ether or methyl-benzyl APEO; Dispersant is sodium phosphate trimer or sodium methylene bis-naphthalene sulfonate; Antifreezing agent is a propane diols; Stabilizing agent is a phenyl glycidyl ether; Dispersion medium is a water.
3. the biopesticide of method preparation according to claim 1 is killed the purposes of cabbage caterpillar or Pomacea canaliculata insect in agricultural production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103602413A CN102487967A (en) | 2011-11-15 | 2011-11-15 | Preparation method of biopesticide |
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| CN2011103602413A CN102487967A (en) | 2011-11-15 | 2011-11-15 | Preparation method of biopesticide |
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| CN102487967A true CN102487967A (en) | 2012-06-13 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103719157A (en) * | 2013-12-12 | 2014-04-16 | 江苏集贤绿色化学科技研究院有限公司 | Biodiesel emulsion |
| CN111184033A (en) * | 2020-01-13 | 2020-05-22 | 乐山师范学院 | Molluscicide compounded by invasive weed eupatorium adenophorum extract and preparation method thereof |
| CN114557355A (en) * | 2022-01-21 | 2022-05-31 | 赤天化科技集团有限公司 | Molluscicidal composition containing metaldehyde and application thereof |
-
2011
- 2011-11-15 CN CN2011103602413A patent/CN102487967A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103719157A (en) * | 2013-12-12 | 2014-04-16 | 江苏集贤绿色化学科技研究院有限公司 | Biodiesel emulsion |
| CN111184033A (en) * | 2020-01-13 | 2020-05-22 | 乐山师范学院 | Molluscicide compounded by invasive weed eupatorium adenophorum extract and preparation method thereof |
| CN114557355A (en) * | 2022-01-21 | 2022-05-31 | 赤天化科技集团有限公司 | Molluscicidal composition containing metaldehyde and application thereof |
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Application publication date: 20120613 |
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