CN109320368A - The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae - Google Patents
The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae Download PDFInfo
- Publication number
- CN109320368A CN109320368A CN201811324230.8A CN201811324230A CN109320368A CN 109320368 A CN109320368 A CN 109320368A CN 201811324230 A CN201811324230 A CN 201811324230A CN 109320368 A CN109320368 A CN 109320368A
- Authority
- CN
- China
- Prior art keywords
- biological agent
- liriomyza bryoniae
- mass fraction
- prevention
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Inorganic Chemistry (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of biological agents and preparation method thereof for preventing and treating liriomyza bryoniae, including formula as below: the mass fraction of microorganism is 0.1 ‰~1 ‰, the mass fraction of shrimp shell meal is 0.1 ‰~10 ‰, the mass fraction of chitin is 0.5 ‰~5 ‰, the mass fraction of collagen is 0.01 ‰~1 ‰, the mass fraction of brown sugar is 1 ‰~20 ‰, the mass fraction of compound amino acid is 1 ‰~50 ‰, the mass fraction of compound fertilizer is 1 ‰~10 ‰, remaining is water；The water is domestic water；The microorganism includes bacillus subtilis.The present invention not only can be reduced the pollution of chemical pesticide, but also can achieve the effect that cost is controllable, and said preparation proportion is simple, easy to use, can ovum to liriomyza bryoniae and larva effectively killed, killing rate is up to 95% or more.
Description
Technical field
The present invention relates to technical field of biological control, more particularly, to a kind of biological agent for preventing and treating liriomyza bryoniae and its preparation
Method.
Background technique
Liriomyza bryoniae is also known as " scrawly handwriting ", is China's plant risk quarantine pest insect.Its larva and adult can all endanger blade,
Larva slips into feeding chlorenchyma, complications under host's blade epidermis and walks, and forms irregular canescence threadiness gallery, inside there is friendship
For the black frass of marshalling；Adult stabs blade cell in blade surface feeding and oviposition, forms the nearly circle of needle point size
Shape stabs " hole ", causes damages.It is found for the first time from liriomyza bryoniae in 1993 in Hainan Province of China, 1994 are classified as internal quarantine object,
So far generally occur in a provinces, autonomous regions and municipalities of China more than 20, it is especially the most serious with Hainan Province.30 can occur every year
In mostly generation, seriously endangers Kidney bean, cucumber, cucurbita pepo, sponge gourd, balsam pear, tomato, capsicum, beet, Chinese cabbage, crowndaisy chrysanthemum, fennel, celery
Equal vegetables, typically result in underproduction 20-30%, and it is anti-to become China pest up to 50% or more or even 80% or more for the underproduction when serious
Control problem.
The liriomyza bryoniae serious reason that causes harm is that host range is wide for liriomyza bryoniae first, almost common up to 33 section, 170 various plants
Vegetables can all occur, and once be difficult to completely remove；Followed by generation is more, and generation overlap is serious, and reproduction speed is fast；
Furthermore drug resistance is serious；Last there are also ceride layers to protect, and liriomyza bryoniae larva is in blade interior potential food, therefore blade face can be formed it
Protection.Currently to the prevention and treatment of liriomyza bryoniae mainly have yellow card's trap adult, pupa basin pupa killing, fumigation kill the physical methods such as adult and trapping at
The chemical methods such as worm.General control method both polluted the environment, and being used for multiple times can also make liriomyza bryoniae develop drug resistance, and reduces and makees
Need a kind of environmental pollution small, the prevention and treatment preparation that can be used for a long time to reduce this problem with effect.
Summary of the invention
The object of the present invention is to provide a kind of biological agents and preparation method thereof for preventing and treating liriomyza bryoniae, both can be reduced chemical agriculture
The pollution of medicine, and can achieve the effect that cost is controllable, and said preparation proportion is simple, it is easy to use, can ovum to liriomyza bryoniae and
Larva is effectively killed, and killing rate is up to 95% or more.
To achieve the above object, the present invention provides a kind of biological agents for preventing and treating liriomyza bryoniae, including formula as below:
The matter that the mass fraction of microorganism is 0.1 ‰~1 ‰, the mass fraction of shrimp shell meal is 0.1 ‰~10 ‰, chitin
Amount score is 0.5 ‰~5 ‰, the mass fraction of collagen is 0.01 ‰~1 ‰, the mass fraction of brown sugar is 1 ‰~20 ‰,
The mass fraction of compound amino acid is 1 ‰~50 ‰, the mass fraction of compound fertilizer is 1 ‰~10 ‰, remaining is water；
The water is domestic water；
The microorganism includes bacillus subtilis.
Preferably, the microorganism further includes Bacillus cercus and bacillus amyloliquefaciens, the bacillus subtilis,
The quantity of said waxy bacillus and bacillus amyloliquefaciens ratio is 0.5~5:0.5~5:0.5~5.
Preferably, the compound amino acid is soybean meal extractive, peanut dregs extract or keratin extract.
Preferably, in the compound fertilizer N, P, K mass ratio be 10~20:10~20:10~20, wherein N, P, K with
Water-soluble component exists, such as: NO3-, NH4 +、PO3-、PO4 3-、HPO4 2-、H2PO4-、P6O18 6-、P2O7 4-、K+, in some examples
In, N, P, K existence form in compound fertilizer are NH4NO3/K2HPO4/KH2PO4, N/P/K=15/15/15, N/P/K=15/15/
17 or N/P/K=15/15/20.
Preferably, the collagen is the powder that Animal Skin is crushed or extracted, wherein the content of collagen
It is 30%~65%, in some examples, collagen is pigskin collagen；In other examples, collagen is animal glue
Former albumen；It is collagen there are also collagen in some examples.
A kind of preparation method of biological agent that preventing and treating liriomyza bryoniae, comprising the following steps:
Firstly, by 0.1 ‰~1 ‰ microorganism, 0.1 ‰~10 ‰ shrimp shell meal, 0.5 ‰~5 ‰ chitin,
0.01 ‰~1 ‰ collagen, 1 ‰~20 ‰ brown sugar, 1 ‰~50 ‰ compound amino acid, 1 ‰~10 ‰ compound fertilizer
It is mixed with water and carries out the obtained biological agent that ferments, the temperature of fermentation is room temperature, and time 48h, condition is bubble aeration, pH value control
System viable count in 6~8, biological agent obtained is not less than 1 × 109A/mL~20 × 109A/mL, preferred biological agent
Middle viable count is 1 × 1010A/mL.This biological agent, which can play interior suction poisoning to the young ovum of liriomyza bryoniae and take oxygen by force, makes its asphyxia
Effect, thus play the role of prevent and treat liriomyza bryoniae.
Preferably, the fermentation temperature is 25 DEG C~35 DEG C, and the rate of the bubble aeration is 20L/min, and dissolved oxygen amount is
20%.
Preferably, the biological agent is applied on prevention and treatment liriomyza bryoniae.
Preferably, in application, the biological agent is 1 mass parts, and water is 1~10 mass parts.
Preferably, the biological agent is sprayed or is sprayed in corresponding crop surface, the application of the biological agent
Amount is 10L/ mus~100L/ mus.
Bacillus subtilis is usually used in the fields such as sewage treatment, aquaculture.Research has shown that withered grass bacterium can be generated
Element, polymyxins, nystatin, gramicidins isoreactivity substance are strong to soil pathogenic bacteria inhibiting effect；Bacillus cercus and
Bacillus amyloliquefaciens viable count is not less than 1 × 1010cfu/mL。
Amino acid is important nutriment, and the main component of compound amino acid used in the present invention is glycine, the third ammonia
Acid, leucine, isoleucine, valine, cystine, cysteine, methionine, serine, threonine, phenylalanine, junket
Propylhomoserin, tryptophan, proline, hydroxyproline, glutamic acid, asparatate etc., source does not limit, and can be animal or plant
Acidolysis or alkaline hydrolysis are made, and are also possible to microbial fermentation and are made, in the present invention, the compound amino acid is by soybean meal extractive, flower
Raw slag extract or keratin extract.
The source of prawn shell powder of the present invention does not limit, and can be the aquatic dynamic rich in chitin of any source of rivers,lakes and seas
The dry product of beyond the region of objective existence shell (also may include meat) crosses 20 mesh~100 meshes particulate powders after crushed.
Therefore, the biological agent and preparation method thereof of the prevention and treatment liriomyza bryoniae of the present invention using the above structure, both can be reduced
It learns to farm the pollution of medicine, and can achieve the effect that cost is controllable, and said preparation proportion is simple, it is easy to use, it can be to liriomyza bryoniae
Ovum and larva are effectively killed, and killing rate is up to 95% or more.
Below by embodiment, technical scheme of the present invention will be described in further detail.
Specific embodiment
The present invention provides a kind of biological agents and preparation method thereof for preventing and treating liriomyza bryoniae, and those skilled in the art can be with
Present disclosure is used for reference, realization of process parameters is suitably modified.In particular, it should be pointed out that all similar substitutions and modifications are to ability
It will be apparent from for the technical staff in domain, they are considered as being included in the present invention.Preparation method of the invention and
Using being described by preferred embodiment, related personnel can obviously not depart from the content of present invention, spirit and scope
It is interior that methods herein and application are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
The present invention provides a kind of biological agents for preventing and treating liriomyza bryoniae, and including formula as below: the mass fraction of microorganism is
0.1 ‰~1 ‰, the mass fraction of shrimp shell meal is 0.1 ‰~10 ‰, the mass fraction of chitin is 0.5 ‰~5 ‰, collagen egg
White mass fraction is 0.01 ‰~1 ‰, the mass fraction of brown sugar is 1 ‰~20 ‰, the mass fraction of compound amino acid is 1 ‰
~50 ‰, the mass fraction of compound fertilizer is 1 ‰~10 ‰, remaining is water, and water is domestic water, and microorganism includes bacillus subtilis
Bacterium, Bacillus cercus and bacillus amyloliquefaciens, bacillus subtilis, Bacillus cercus and bacillus amyloliquefaciens number
Amount than be 0.5~5:0.5~5:0.5~5, compound amino acid be soybean meal extractive, peanut dregs extract or keratin extract,
The mass ratio of N, P, K are 10~20:10~20:10~20 in compound fertilizer, and wherein N, P, K are with the presence of water-soluble component, such as:
NO3-、NH4 +、PO3-、PO4 3-、HPO4 2-、H2PO4-、P6O18 6-、P2O7 4-、K+, in some instances, N, P, K in compound fertilizer are deposited
It is NH in form4NO3/K2HPO4/KH2PO4, N/P/K=15/15/15, N/P/K=15/15/17 or N/P/K=15/15/20,
Collagen is the powder that Animal Skin is crushed or extracted, and wherein the content of collagen is 30%~65%, Yi Xieshi
In example, collagen is pigskin collagen；In other examples, collagen is cow leather collagen；There are also some examples
Middle collagen is collagen.
A kind of preparation method of biological agent that preventing and treating liriomyza bryoniae, comprising the following steps:
Firstly, by 0.1 ‰~1 ‰ microorganism, 0.1 ‰~10 ‰ shrimp shell meal, 0.5 ‰~5 ‰ chitin,
0.01 ‰~1 ‰ collagen, 1 ‰~20 ‰ brown sugar, 1 ‰~50 ‰ compound amino acid, 1 ‰~10 ‰ compound fertilizer
It being mixed with water and carries out the obtained biological agent that ferments, the temperature of fermentation is room temperature, generally 25 DEG C~35 DEG C, time 48h, condition
For bubble aeration, 6~8, the rate of bubble aeration is 20L/min, dissolved oxygen amount 20%, biological agent obtained for pH value control
Middle viable count is not less than 1 × 109A/mL~20 × 109A/mL, viable count is 1 × 10 in preferred biological agent10A/mL.
This biological agent can play the role of interior suction poisoning to the young ovum of liriomyza bryoniae and taking oxygen by force makes it suffocate, so that it is latent to play prevention and treatment spot
The effect of fly, in application, biological agent is 1 mass parts, and water is 1~10 mass parts, sprays or be sprayed at correspondence for biological agent
Crop surface on, the amount of application of the biological agent is 10L/ mus~100L/ mus.
Embodiment 1
Fetch water 1000L, bacillus subtilis 0.1kg, shrimp shell meal 0.1kg, chitin 0.5kg, collagen 0.01kg, red
Sugared 3kg, compound amino acid 3kg, compound fertilizer 3kg, wherein collagen is pigskin collagen, and compound amino acid is dregs of beans extraction
Object, N/P/K=15/15/15 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin, collagen, brown sugar, compounded amino
Acid, compound fertilizer's bubble aeration that is added to the water are cultivated, and cultivation temperature is 25 DEG C of room temperature, incubation time 48h, pH value control
6~8, the rate of bubble aeration is 20L/min, and dissolved oxygen amount is 20% to get biological agent.
Embodiment 2
Fetch water 1000L, Bacillus cercus 0.1kg, shrimp shell meal 0.5kg, chitin 2kg, collagen 0.1kg, brown sugar
5kg, compound amino acid 3kg, compound fertilizer 1kg, wherein collagen is cow leather collagen, and compound amino acid is keratin extraction
Object, N/P/K=15/15/20 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin, collagen, brown sugar, compounded amino
Acid, compound fertilizer's bubble aeration that is added to the water are cultivated, and cultivation temperature is 28 DEG C of room temperature, incubation time 48h, pH value control
6~8, the rate of bubble aeration is 20L/min, and dissolved oxygen amount is 20% to get biological agent.
Embodiment 3
Fetch water 1000L, bacillus amyloliquefaciens 0.5kg, shrimp shell meal 1kg, chitin 1kg, collagen 0.5kg, brown sugar
3kg, compound amino acid 3kg, compound fertilizer 3kg, wherein collagen is collagen, and compound amino acid is dregs of beans extraction
Object, N/P/K=15/15/15 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin, collagen, brown sugar, compounded amino
Acid, compound fertilizer's bubble aeration that is added to the water are cultivated, and cultivation temperature is 29 DEG C of room temperature, incubation time 48h, pH value control
6~8, the rate of bubble aeration is 20L/min, and dissolved oxygen amount is 20% to get biological agent.
Embodiment 4
Fetch water 1000L, bacillus subtilis 0.2kg, Bacillus cercus 0.5kg, shrimp shell meal 1kg, chitin 3kg, glue
Former albumen 0.5kg, brown sugar 3kg, compound amino acid 1kg, compound fertilizer 2kg, wherein collagen is pigskin collagen, compound ammonia
Base acid is soybean meal extractive, N/P/K=15/15/17 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin, collagen,
Brown sugar, compound amino acid, compound fertilizer's bubble aeration are cultivated, and cultivation temperature is 28 DEG C of room temperature, incubation time 48h, pH value
6~8, the rate of bubble aeration is 20L/min for control, and dissolved oxygen amount is 20% to get biological agent.
Embodiment 5
Fetch water 1000L, bacillus subtilis 0.6kg, bacillus amyloliquefaciens 1kg, shrimp shell meal 2kg, chitin 0.5kg,
Collagen 0.01kg, brown sugar 2kg, compound amino acid 5kg, compound fertilizer 3kg, wherein collagen is cow leather collagen, multiple
Conjunction amino acid is peanut dregs extract, N/P/K=15/15/15 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin, glue
Former albumen, brown sugar, compound amino acid, the compound fertilizer bubble aeration that is added to the water are cultivated, and cultivation temperature is 33 DEG C of room temperature, training
Supporting the time is 48h, and 6~8, the rate of bubble aeration is 20L/min for pH value control, and dissolved oxygen amount is 20% to get biological agent.
Embodiment 6
Fetch water 1000L, Bacillus cercus 0.1kg, bacillus amyloliquefaciens 0.5kg, shrimp shell meal 0.5kg, chitin
0.5kg, collagen 1kg, brown sugar 5kg, compound amino acid 1kg, compound fertilizer 2kg, wherein collagen is cow leather collagen,
Compound amino acid is keratin extract, N/P/K=15/15/20 in compound fertilizer.By mentioned microorganism, shrimp shell meal, chitin,
Collagen, brown sugar, compound amino acid, the compound fertilizer bubble aeration that is added to the water are cultivated, and cultivation temperature is 27 DEG C of room temperature,
Incubation time is 48h, and 6~8, the rate of bubble aeration is 20L/min for pH value control, and dissolved oxygen amount is 20% to get biology system
Agent.
Embodiment 7
Fetch water 1000L, bacillus subtilis 0.5kg, Bacillus cercus 1kg, bacillus amyloliquefaciens 0.6kg, shrimp shell
Powder 1kg, chitin 0.5kg, collagen 0.01kg, brown sugar 5kg, compound amino acid 1kg, compound fertilizer 1kg, wherein collagen
For collagen, compound amino acid is keratin extract, N/P/K=15/15/20 in compound fertilizer.By mentioned microorganism,
Shrimp shell meal, chitin, collagen, brown sugar, compound amino acid, the compound fertilizer bubble aeration that is added to the water are cultivated, and are cultivated
Temperature is 35 DEG C of room temperature, incubation time 48h, and pH value control is 6~8, and the rate of bubble aeration is 20L/min, and dissolved oxygen amount is
20% to get biological agent.
Biological agent made from the various embodiments described above, through detecting, inoculum density such as table 1:
Table 1 is the inoculum density of each embodiment
| Project | Inoculum density |
| Embodiment 1 | 2.8×1012 |
| Embodiment 2 | 6.7×1011 |
| Embodiment 3 | 8.1×1012 |
| Embodiment 4 | 2.1×1012 |
| Embodiment 5 | 1.3×1013 |
| Embodiment 6 | 4.3×1011 |
| Embodiment 7 | 9.0×1012 |
The result shows that the biological agent provided in each formulation Example of the present invention all has very high inoculum density.
Biological agent in embodiment 1-7 is diluted to 10 using sterile water6Cfu/mL detects its prevention and treatment to liriomyza bryoniae
Effect.
Field trial example 1
The test for preventing and treating cowpea (dragon's fountain No.1 cowpea, Jinan dragon's fountain seed Co., Ltd) Americal rice leaf miner.Place is located at
Three power source ecological agriculture garden greenhouse of Hainan Province Sanya betel nut paddy is administered in test on January 4th, 2018, and at this moment cowpea is just located
In quickly stretching the climing phase, and Americal rice leaf miner occurs universal, and every plant has aggrieved leaf.
Test sets biological agent stoste, biological agent 1:1 dilution, biological agent 1:2 dilution, 5% avermectin cream
Oily 1500 times of liquid and clear water control totally 5 processing.20 square metres of plot area, random alignment, in triplicate.On January 4th, 2018
Make overground part blade face using knapsack electric sprayer afternoon uniformly to spray, medical fluid is allowed uniformly to be stained with blade face and lower drop, amount of application
60 liters/mu.
Investigation method and statistics: carrying out before spray, using 5 point samplings, 5 points of every cell selection, and every 5 piers, often
Pier 1 expansion blade of listed investigation, every cell investigate 25 expansion leaves, record larva sum, as insect population before medicine, spray
Afterwards 2,6,10, day is respectively investigated once, records borer population living.Revision insect recluced rate is calculated, Revision insect recluced rate, i.e. control efficiency are corrected.
Calculation formula:
Revision insect recluced rate=(borer population living after insect population-application before being administered)/insect population × 100% before being administered
Control efficiency (correction Revision insect recluced rate)=(check plot Revision insect recluced rate-treatment region Revision insect recluced rate)/(1- control
Area's Revision insect recluced rate) × 100%
To the drug effect statistical result of 5 processing in test, such as table 2:
2 biological agent of table prevents and treats cowpea Americal rice leaf miner and tests drug effect statistical form
Biological agent stoste and its 1:1,1:2 dilution the 6th day control efficiency to cowpea Americal rice leaf miner after spray
(correction Revision insect recluced rate) is respectively as follows: 100%, 100%, 96.89%, 1500 times of liquid of significantly larger than 5% abamectin emulsifiable concentrate
66.5% control efficiency, the 10th day correction Revision insect recluced rate is respectively 99.83%, 99.12%, 89.84% after application, even more
Much higher than the control efficiency of 5% 1500 times of liquid 54.50% of abamectin emulsifiable concentrate.Therefore, biological agent stoste and biological agent 1:
1,1:2 dilution significant effect in terms of directly killing each instar larvae of cowpea Americal rice leaf miner and long-term control.
Field trial example 2
The test for preventing and treating cucumber (green No. 4 of saliva) Americal rice leaf miner.It is raw that place is located at three power source of Hainan Province Sanya betel nut paddy
State Agricultural Park greenhouse.It is administered in test on December 31st, 2018, at this moment cucumber Americal rice leaf miner is early period of origination, and every plant equal
It comes to harm.
Test sets biological agent stoste, biological agent 1:1 dilution, biological agent 1:2 dilution, 5% avermectin cream
Oily 1500 times of liquid and clear water control totally 5 processing.30 square metres of plot area, number 15, random alignment, in triplicate.
Afternoon on December 31st, 2018 makees overground part blade face using knapsack electric sprayer and uniformly sprays, and medical fluid is allowed uniformly to be stained with blade face
And lower drop, amount of application are 60 liters/mu.
Investigation method and statistics: carrying out before spray, using 5 point samplings, 5 points of every cell selection, and 2 plants every, often
Strain 3 expansion blades of listed investigation, record larva sum, investigate as insect population before medicine.3 after spray, respectively investigate within 7,14 days
Once, borer population dead or living is recorded.Calculate the death rate, corrected mortality, Revision insect recluced rate, correction Revision insect recluced rate (prevention and treatment effect
Fruit), the lethal effect of the Americal rice leaf miner for evaluating different agents processing pair.Security survey: investigation in 1,3 day is made after application
Whether there is or not symptom of chemical damage for object, the safety with correct evaluation test medicament to crop.
Calculation formula:
Revision insect recluced rate=(borer population living after insect population-application before being administered)/insect population × 100% before being administered
Control efficiency (correction Revision insect recluced rate)=(check plot Revision insect recluced rate-treatment region Revision insect recluced rate)/(1- control
Area's Revision insect recluced rate) × 100%
To the drug effect statistical result of 5 processing in test, such as table 3:
3 biological agent of table prevents and treats cucumber Americal rice leaf miner and tests drug effect statistical form
Observation in 1 to 3 day occurs without key after spray.Biological agent stoste and biological agent 1:1,1:2 dilution are spraying
The 7th day control efficiency (correction Revision insect recluced rate) to cucumber Americal rice leaf miner is respectively as follows: 100%, 100%, 95.74% afterwards,
Far better than the control efficiency of 5% 1500 times of liquid 59.06% of abamectin emulsifiable concentrate, fortnight control efficiency is respectively after application
97.98%, 95.44%, 85.27% is even more the control efficiency much higher than 5% 1500 times of liquid 52.03% of abamectin emulsifiable concentrate.It can
See biological agent stoste and biological agent 1:1,1:2 dilution to the direct killing effect of each instar larvae of cowpea Americal rice leaf miner and length
Phase control action was significant, up to 14 days or more.And biological agent environmental pollution itself is small, can slow down the production of pest resistance to insecticide
It is raw, there is wide promotion prospect.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is limited,
Although the present invention is described in detail with reference to preferred embodiments, those skilled in the art should understand that: its according to
So can be with modification or equivalent replacement of the technical solution of the present invention are made, and these modifications or equivalent replacement cannot also make to repair
Technical solution after changing is detached from the spirit and scope of technical solution of the present invention.
Claims (10)
1. a kind of biological agent for preventing and treating liriomyza bryoniae, which is characterized in that including formula as below:
The mass fraction of microorganism is 0.1 ‰~1 ‰, the mass fraction of shrimp shell meal is 0.1 ‰~10 ‰, the quality of chitin point
Number is 0.5 ‰~5 ‰, the mass fraction of collagen is 0.01 ‰~1 ‰, the mass fraction of brown sugar is 1 ‰~20 ‰, compound
The mass fraction of amino acid is 1 ‰~50 ‰, the mass fraction of compound fertilizer is 1 ‰~10 ‰, remaining is water；
The water is domestic water；
The microorganism includes bacillus subtilis.
2. the biological agent of prevention and treatment liriomyza bryoniae according to claim 1, it is characterised in that: the microorganism further includes wax-like
Bacillus and bacillus amyloliquefaciens, the bacillus subtilis, said waxy bacillus and the solution starch gemma bar
The quantity ratio of bacterium is 0.5~5:0.5~5:0.5~5.
3. the biological agent of prevention and treatment liriomyza bryoniae according to claim 1, it is characterised in that: the compound amino acid is dregs of beans
Extract, peanut dregs extract or keratin extract.
4. the biological agent of prevention and treatment liriomyza bryoniae according to claim 1, it is characterised in that: N, P, K in the compound fertilizer
Mass ratio is 10~20:10~20:10~20, and wherein N, P, K are with the presence of water-soluble component.
5. the biological agent of prevention and treatment liriomyza bryoniae according to claim 1, it is characterised in that: the collagen is Animal Skin
The powder for being crushed or being extracted, wherein the content of collagen is 30%~65%.
6. a kind of preparation method of the biological agent of prevention and treatment liriomyza bryoniae as described in any one in claim 1-5, which is characterized in that
The following steps are included:
Firstly, by 0.1 ‰~1 ‰ microorganism, 0.1 ‰~10 ‰ shrimp shell meal, 0.5 ‰~5 ‰ chitin, 0.01 ‰~
1 ‰ collagen, 1 ‰~20 ‰ brown sugar, 1 ‰~50 ‰ compound amino acid, 1 ‰~10 ‰ compound fertilizer mixed with water
Carry out the obtained biological agent that ferments, the temperature of fermentation is room temperature, time 48h, and condition is bubble aeration, pH value control 6~
8。
7. the preparation method of the biological agent of prevention and treatment liriomyza bryoniae according to claim 6, it is characterised in that: the fermentation temperature
Degree is 25 DEG C~35 DEG C, and the rate of the bubble aeration is 20L/min, dissolved oxygen amount 20%.
8. the preparation method of the biological agent of prevention and treatment liriomyza bryoniae according to claim 7, it is characterised in that: the biology system
Agent is applied on prevention and treatment liriomyza bryoniae.
9. the preparation method of the biological agent of prevention and treatment liriomyza bryoniae according to claim 8, it is characterised in that: in application when institute
Stating biological agent is 1 mass parts, and water is 1~10 mass parts.
10. the preparation method of the biological agent of prevention and treatment liriomyza bryoniae according to claim 8, it is characterised in that: by the life
Object preparation is sprayed or is sprayed in corresponding crop surface, and the amount of application of the biological agent is 10L/ mus~100L/ mus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811324230.8A CN109320368A (en) | 2018-11-08 | 2018-11-08 | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811324230.8A CN109320368A (en) | 2018-11-08 | 2018-11-08 | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109320368A true CN109320368A (en) | 2019-02-12 |
Family
ID=65261194
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811324230.8A Pending CN109320368A (en) | 2018-11-08 | 2018-11-08 | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109320368A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110833075A (en) * | 2019-10-11 | 2020-02-25 | 北京泰克美高新技术有限公司 | Compound preparation and application thereof in treating pine plague wood infected with pine wilt disease |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186558A (en) * | 2014-07-31 | 2014-12-10 | 泗阳县聚丰生态农业发展有限公司 | Agent used for preventing and treating liriomyza sativae of okra |
| CN104230580A (en) * | 2014-08-21 | 2014-12-24 | 上海亨康生物科技有限公司 | Composite microbial disease/insect-resistant agent for green vegetables and manufacturing method thereof |
| CN104738092A (en) * | 2014-05-04 | 2015-07-01 | 陕西兰迪生物技术有限公司 | Composite preparation for preventing and treating cyanosis as well as preparation method and application method of composite preparation |
| CN105410032A (en) * | 2015-12-21 | 2016-03-23 | 云南省微生物发酵工程研究中心有限公司 | Microbial trapping agent and application thereof |
| CN107287139A (en) * | 2017-08-03 | 2017-10-24 | 北京泰克美高新技术有限公司 | A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved |
-
2018
- 2018-11-08 CN CN201811324230.8A patent/CN109320368A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738092A (en) * | 2014-05-04 | 2015-07-01 | 陕西兰迪生物技术有限公司 | Composite preparation for preventing and treating cyanosis as well as preparation method and application method of composite preparation |
| CN104186558A (en) * | 2014-07-31 | 2014-12-10 | 泗阳县聚丰生态农业发展有限公司 | Agent used for preventing and treating liriomyza sativae of okra |
| CN104230580A (en) * | 2014-08-21 | 2014-12-24 | 上海亨康生物科技有限公司 | Composite microbial disease/insect-resistant agent for green vegetables and manufacturing method thereof |
| CN105410032A (en) * | 2015-12-21 | 2016-03-23 | 云南省微生物发酵工程研究中心有限公司 | Microbial trapping agent and application thereof |
| CN107287139A (en) * | 2017-08-03 | 2017-10-24 | 北京泰克美高新技术有限公司 | A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110833075A (en) * | 2019-10-11 | 2020-02-25 | 北京泰克美高新技术有限公司 | Compound preparation and application thereof in treating pine plague wood infected with pine wilt disease |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106544278A (en) | A kind of pathogenic fungi for Blatta seu periplaneta Biological control | |
| CN105198602A (en) | Fertilizer with natural insecticidal effect and preparation method thereof | |
| CN106350464A (en) | Microbial agent for preventing and treating cumquat huanglongbing and preparation method thereof | |
| CN106591203B (en) | A kind of Paenibacillus polymyxa KM2501-1 and its application | |
| JP2020531583A (en) | Acaricides and their applications | |
| WO2017107872A1 (en) | Microbial attractant, and use | |
| CN104692957A (en) | Biological organic fertilizer containing bacillus subtilis and application thereof | |
| CN101589716A (en) | The compounded pesticides of Osthole and matrine and application thereof | |
| CN106135291A (en) | A kind of prevent and treat soil-borne disease, improve crop cold resistance compositions | |
| CN105061078A (en) | Health bio-organic fertilizer and preparation method thereof | |
| CN102461513A (en) | Preparation and method for controlling plant pathogenic nematodes | |
| CN102257935A (en) | Method for controlling plant diseases through cocktail therapy | |
| CN102907458B (en) | Biological mosquitocide and preparation method thereof | |
| CN104304323B (en) | The method utilizing entomopathogenic nematode and matrine combination preventing and treating black cutworm | |
| CN103719084B (en) | Application of beta-aminobutyric acid in inducing tea tree to generate resistance on tea geometrid | |
| CN103734117B (en) | A kind of method of ecological organic tea place control false eye leafhopper nymph | |
| CN109320368A (en) | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae | |
| WO2017067169A1 (en) | Compound pesticide for preventing tetranychus urticae | |
| KR101826310B1 (en) | Nematocidal composition comprising Chitosan and Actinomycetes | |
| CN109650997A (en) | A kind of plant root-knot nematode administering method | |
| CN115521357A (en) | Agricultural biological weeding enzyme | |
| CN105272693A (en) | Biological insecticidal organic fertilizer and preparation method thereof | |
| CN108690818A (en) | A kind of microbial bacterial agent and preparation method thereof | |
| JPH01113306A (en) | Controlling drug against pomacea canaliculata lamarck and method for controlling | |
| CN103250486A (en) | Method for biologically preventing root-knot nematode by taking cyanogenic glycoside rich crop straws as raw materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190212 |