CN105410032A - Microbial trapping agent and application thereof - Google Patents
Microbial trapping agent and application thereof Download PDFInfo
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- CN105410032A CN105410032A CN201510958305.8A CN201510958305A CN105410032A CN 105410032 A CN105410032 A CN 105410032A CN 201510958305 A CN201510958305 A CN 201510958305A CN 105410032 A CN105410032 A CN 105410032A
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- trapping bait
- bacillus amyloliquefaciens
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- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 13
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- 244000005700 microbiome Species 0.000 claims description 48
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- 239000002609 medium Substances 0.000 claims description 24
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- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
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- 208000027418 Wounds and injury Diseases 0.000 description 2
- YKPQYKBIAFGWBT-KAMYIIQDSA-N [(Z)-18-hydroxyoctadec-9-en-7-yl] acetate Chemical compound CCCCCCC(C\C=C/CCCCCCCCO)OC(C)=O YKPQYKBIAFGWBT-KAMYIIQDSA-N 0.000 description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a microbial trapping agent and application thereof. An active component of the trapping agent is bacillus amyloliquefaciens YN2010-KC (CGMCC No. 5722) fermentation liquor in which the living bacteria count is larger than or equal to 3*10<9>cfu/ml. The trapping agent can induce trypetid, vinegar fly and vegetable leafminer to eat due to a particular smell emitted thereby and catch both female and male flies, is long in action period and low in cost, does not contain any chemical toxic agents, does not kill and wound any other non-target living beings and belongs to an environment-friendly biological trapping agent. Euaresta aequalis is trapped: the quantity of the Euaresta aequalis is increased by 18.81 to 23.71% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 37.78% to 30 times compared with that of the control. Tetradacus citri is trapped: the quantity of the Tetradacus citri is increased by 64.43% compared with that of the control, and the quantity of female flies trapped by the trapping agent is increased by 33.25 times. The vegetable leafminer is trapped: the female fly rate accounts for 55 to 60%.
Description
Technical field
The invention belongs to agricultural science injurious insect control technical field, be specifically related to a kind of microorganism trapping bait, and the application in trapping male and female fruit fly, liriomyza bryoniae.
Background technology
Diptera (Diptera) is 1 order of Arthropoda (Arthropoda), Mandibulata (Mandibulata), Insecta (Insecta), Pterigota (Pterygota), is to be only second to coleoptera, Lepidoptera, hymenopteran the fourth-largest order in Insecta.Known 85000 kinds of the world, distribution on global.China known more than 4000 plants.Dipteral insect has a pair wing and a pair haltere, comprises mosquito, fly, horsefly.Some monoids of common diptera such as kind of a fly, leaf are dived the larva of fly, fruit fly, wheat cecidomyiia etc., are all the important pests of agricultural.Agromyzidae is caused harm multiple leguminous plant; The numerous species of Tephritidae is caused harm oranges and tangerines, pears, peach etc.
Fruit fly belongs to diptera Tephritidae (Tephritidae), is class important pests fruits and vegetables class crop being caused to heavy losses.Tephritidae is known about 500 genus up to now, more than 4500 plant, wherein, the monoid that fruits and vegetables class is harmful to trypetid reaches 15 genus, 22 subgenus, more than 150 kinds, [Bactroceradorsalis (Hendel)] is closed with dacus dorsalis in China, melon trypetid [Bactroceracucurbitae (CoqilLett)], citrus fruit flies [Bactroceraminax (Enderlein)] etc. are comparatively common, are mainly distributed in the provinces such as Hunan, Hubei, Sichuan, Chongqing, Guizhou, Fujian, Guangdong, Guangxi, Hainan, Yunnan, Taiwan.The host range of harmful trypetid is very wide, relates to economic fruit class plant and amounts to 24 sections, 42 genus: vegetable plants 4 section, 16 belongs to (wild host plant is not included).Fruit fly fertility is strong, harmfulness is large.In trypetid distribution or Endemic Area, host volatiles rate of damage average out to 25-45%, up to 80% time serious, even has no harvest, and brings heavy economic losses to fruits and vegetables class crop, has had a strong impact on peasant and has increased production extra earning.
At present, the method for preventing and treating Fruit fly mainly contains cultural control, chemical control, physical control and biological control etc.Wherein cultural control comprises band wormed fruit reality, fruit bagging and the pupa etc. that goes out of turning over winter regularly removed in plantation; Chemical control methods mainly sprays Bait on Killing trypetid; The technology that physical control measure adopts trypetid trapping bait and poison bait to combine, at present, for preventing and treating the trapping bait mainly chemosynthesis trapping bait of trypetid, as lured ketone (cuelure), methyl eugenol (methyleugenol) etc.Take a lot of work due to during cultural control measure expense, effect is bad; Chemical control not only easily makes trypetid develop immunity to drugs, and is harmful to people, animal, easily produces the problem of the environmental pollutions such as residue of pesticide; There is again the problems such as volatilization is fast, the lasting period is short, medication is frequent in property trapping bait in actual applications, and what do not reach control borer fruit rate prevents and treats object, and current most widely used general trapping bait methyl eugenol only lures male worm etc., and its application is restricted.
Sugar-vinegar liquid, female sex hormone, protein three major types lure is had both at home and abroad for trypetid.Sugar-vinegar liquid is time-consuming, effort, and cost is higher; Female sex hormone can only lure male worm, and due to the capable multiple mating of male worm energy, preventive effect is often not ideal; Protein-based trapping bait can lure female male worm, effective.But the protein lure at present on overseas market is pure animal protein, and production cost is high.
Americal rice leaf miner (LiriomyzasativaeBlanchard) also belongs to diptera Agromyzidae (Agromyzidae) Liriomyza (Liriomyza), being commonly called as vegetable leafminer, map worm, scrawly handwriting etc., is the dangerous pest that leaf mining is caused harm.China early than in December, 1993 at Sanya, Hainan Province Late Cambrian, Americal rice leaf miner northwards spreads gradually subsequently, 1994 at Kunming, Yunnan occurring and damage, nineteen ninety-five occurs on Shanghai and Nanjing and other places, 1996 in Shandong, Beijing population outbreak, 1997 in Henan, Shanxi occurs, 1998 northeastward, the Inner Mongol causes disaster, except Tibet, all there is distribution in the whole nation.Its host plant reaches more than 200 and plants, and wherein vegetables are injured the heaviest with solanaceous vegetables (except pimento, capsicum), beans, melon, Carrot family etc.
Adult of Liriomyza Sativae Blanchard is small-sized fly class, body black, and long 1.3 ~ 2.3 millimeters of body, in 8.7 ~ 12.6 days life-spans, every female all one's life lays eggs 70 ~ 125 pieces, and incubation rate is up to more than 90%.Larva milky is to faint yellow, and long 3 millimeter, year bred for 15 ~ 20 generations.Enter winter in greenhouse by solar heat, can endanger in the four seasons, mainly contain 2 hazard booms, appear at 5 ~ June and 9 ~ October respectively, year can reach 20 generations more than from generation to generation.Adult, larva all can endanger, female adult pest acanthopore on host's blade also takes food wherein and lays eggs, larva is potential food mesophyll or petiole after the subcutaneous hatching of blade table, produce irregular snakelike white gallery, destroy leaf chlorophyll, affect photosynthesis, time serious, the withered and yellow even whole strain that comes off of blade is withered; Cause seedling stage and ruin seedling.Endanger serious field to be injured strain 100%, blade is injured 70%.Abound with phase custard squash, after discovery harm, within about 7 days, suffer all mesophyll, destruction in about 15 days.Larva and adult also can propagate some virus disease by taking food.
At present; along with the change of cropping system; continue the continuous expansion of warm winter phenomenon and open country and vegetable at protected field area; Liriomyza has become and various vegetables has endangered one of especially big, disastrous insect preventing and treating exceptionally poverty-stricken difficulty; annual generation is many from generation to generation and overlap is chaotic; its spread in china is fast, and autumn land for growing field crops the end of spring and the beginning of summer, protecting field occurred especially serious the whole year.The Protectorate cultivation of vegetables is that vegetable leafminer provides good hibernacle.This worm not only can survive the winter in booth, and can in canopy occurrence injury, become the important worm source in outdoor vegetable field.Therefore, the control done well in booth can prevent this worm after taking off canopy effectively to outdoor vegetable diffusion harm.
Because this worm generation overlap phenomenon is serious, pesticide resistance is strong, more difficult control in production.At present, in production to the control of Americal rice leaf miner still mainly based on chemical control, in order to solve the food-safety problem that chemical pesticide for many years brings, bio-control method should be utilized to control insect pest, but biological prevention does not also have real application in production and performance benefit, and find with strong points, preventive effect is high, and to people, animal safety, do not have the new lure of the one of environmental pollution extremely urgent.
Summary of the invention
For the needs of prior art, the object of this invention is to provide a kind of be applicable to trap trypetid and fruit bat class pest, can male and female with the novel microbial trapping bait of catching.
For achieving the above object, technical scheme of the present invention is as follows:
1. a microorganism trapping bait, its active component is the zymotic fluid of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722.
2. a kind of microorganism trapping bait according to technical scheme 1, the zymotic fluid of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 prepares by the following method:
(1) after bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 being activated, by 5 ~ 10% inoculum concentrations, described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, under cultivation temperature 35 DEG C, rotating speed 120rpm/min condition, 36 ~ 48h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2;
(2) by 10 ~ 12% inoculum concentrations, primary seed solution is transferred to the secondary seed medium in seeding tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth of following mass fraction: corn flour 0.3 ~ 0.5%, analysis for soybean powder 1 ~ 1.5%, sucrose 0.2 ~ 0.5%, peptone 0.1 ~ 0.3%, fish meal 0.1 ~ 0.3%, MgSO
47H
2o0.02 ~ 0.04%, CaCO
30.4 ~ 0.8%, (NH
4)
2sO
40.03 ~ 0.06%, surplus is water, pH7.0 ~ 7.2;
(3) by 10 ~ 12% inoculum concentrations, secondary seed solution is inoculated into the fermentation medium in fermentation tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, throughput is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, You effect Jun Shuo≤3 × 10 alive of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 in the zymotic fluid after cultivation
9during cfu/ml, this zymotic fluid is the zymotic fluid of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722, and described fermentation medium is identical with step (2) described secondary seed medium.
3. the microorganism trapping bait described in technical scheme 1 or 2 is in the application of control Fruit fly.
4. the microorganism trapping bait described in technical scheme 1 or 2 is in the application of control Americal rice leaf miner.
5. the microorganism trapping bait according to technical scheme 3 is in the application of control Fruit fly, described application loads in plastic bottle by described microorganism trapping bait, the charge weight of every bottle is 50 ~ 100mL, screw bottle cap, uniform 4 holes on the bottle wall apart from liquid level 1 ~ 3cm in bottle, the aperture in described each hole is 4 ~ 6mm; Plastic bottle is hung on overhead 0.5 ~ 1.5 meter of, every mu is hung plastic bottle 8 ~ 10, adjacent plastic bottle interval 5 ~ 7m; In plastic bottle, described microorganism trapping bait 25 ~ 50mL is added once every 20 ~ 30 days.
Compared with prior art, the present invention has following beneficial effect:
1, the special odor that product of the present invention gives out can lure trypetid and fruit bat to take food, can male and female with catching, the action period is long, and disposable feeding intake can two weeks interior holding effects, and cost is low, has stronger competitiveness.Dacus dorsalis is traped: special fruit bat attractant more auspicious than fruit increases by 23.71%, increases by 18.81% than methyl eugenol.The female worm lured than the auspicious special fruit bat attractant of fruit increases by 37.78%, and the female worm lured than methyl eugenol increases by 30 times.Citrus fruit fly is traped: increase by 64.43% than methyl eugenol, the citrus fruit fly female worm number ratio methyl eugenol of trapping increases by 33.25 times.Trapping to Americal rice leaf miner: female worm rate accounts for 55 ~ 60%, creates unforeseeable technique effect.Methyl eugenol lures less than liriomyza bryoniae, can only be contrast with sugar-vinegar liquid, and the latter also can lure female male worm, and just total worm amount is more much lower than this product.
2, do not contain any chemical toxic medicament in product of the present invention, do not kill and do not kill and wound any other non-target organism, belonging to environmentally friendly fruit fly trapping bait.
3, product of the present invention completely can substituted chemistry insecticide, greatly reduces chemical pesticide consumption, thus reduces chemical pesticide to the negative effect of natural enemy insect, without pesticide resistance.
4, product of the present invention can improve agricultural production quality and yield, and to ecological safety, ecological benefits are remarkable, uses simple.
Bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC of the present invention is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
Embodiment
Below in conjunction with embodiment, a kind of microorganism trapping bait provided by the invention is described in detail; but the present invention is not construed as limiting; those skilled in the art are improving technique parameter on this basis, changes concrete auxiliary material type etc. and replaces and change, still belong to protection scope of the present invention.Each embodiment is conventional method without specified otherwise.
The acquisition of embodiment 1 bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KC, preservation
1, the acquisition of bacillus amyloliquefaciens YN2010-KC of the present invention
1.1 material
Pick up from the soil around the Rhizosphere of Crops of Yunnan Prov Agriculture University Hou Shan farm.
1.2 medium preparing
LB solid culture medium and PSA medium (potato sucrose agar medium) are melted, pours sterilizing culture dish respectively into, cooling, make LB and PSA dull and stereotyped.
1.3 are separated cultivation
Adopting dilution plate partition method to be separated with plate streaking, is YN2010-KC by a Strain Designation of separation and purification gained.
2, the qualification of bacillus amyloliquefaciens YN2010-KC
2.1, Morphological Identification
Get bacterial strain YN2010-KC to cultivate on LB medium, cultivate 36 hours, carry out morphologic observation qualification for 37 DEG C, thalline is shaft-like, end circle, and peritrichous, tool motility, produce oval gemma; On LB and PSA flat board, bacterium colony circle, weak tea brown, bacterium colony central color slightly dark, protuberance, dry tack free has fold.(eastern elegant pearl etc. are write with " common bacteria system identification handbook ", Science Press, calendar year 2001) in describe bacillus morphological feature basically identical, tentatively judge that bacterial strain YN2010-KC belongs to bacillus (Bacillusspp.).
2.2, Physiology and biochemistry qualification
Bacterial strain Gram’s staining, spore staining, Starch Hydrolysis, glucose utilization, citrate utilization, V-P reaction, catalase reaction, nitrate reduction, gelatin liquefaction etc. are all positive.
3, the preservation of bacillus amyloliquefaciens YN2010-KC
Through above-mentioned identify show be separated obtain bacterial strain be bacillus amyloliquefaciens (Bacillusamyloliquefaciens), its code name is YN2010-KC, described bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
The preparation method of embodiment 2 microorganism trapping bait of the present invention, comprises the following steps:
(1) bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 that-80 DEG C are preserved is seeded on LB liquid medium, cultivate 24h for 35 DEG C, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 that must activate, by 10% inoculum concentration, described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, cultivation temperature 35 DEG C, under rotating speed 120rpm/min condition, 36h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2,
(2) by 10% inoculum concentration, primary seed solution is transferred to the secondary seed medium in seeding tank, at 35 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Obtain secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth of following mass fraction: corn flour 0.5%, analysis for soybean powder 1%, sucrose 0.5%, peptone 0.3%, fish meal 0.1%, MgSO
47H
2o0.02%, CaCO
30.4%, (NH
4)
2sO
40.03%, surplus is water, pH7.0 ~ 7.2; Described secondary seed medium, in 121 DEG C of sterilizing 30min, is waited to be cooled to 30 DEG C, access primary seed solution;
(3) by 10% inoculum concentration, secondary seed solution is inoculated into the fermentation medium in fermentation tank, at 35 DEG C, rotating speed 100rpm/min, throughput is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, You effect Jun Shuo≤3 × 10 alive of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 in the zymotic fluid after cultivation
9during cfu/ml, packed by zymotic fluid medicinal plastic bottle, be described microorganism trapping bait, described fermentation medium is identical with step (2) described secondary seed medium.
Microorganism trapping bait of the present invention is prepared by the preparation method of the microorganism trapping bait described in embodiment 2.
The comparative trial that embodiment 3 microorganism trapping bait of the present invention and other lure are traped dacus dorsalis
Trap effect test is carried out in Yuanjiang county citrus orchard, simultaneously with " fruit Rui Te " fruit bat attractant, (belonging to albumen bait formulation, commercially available), methyl eugenol (attribute lure, commercially available) and water (blank) contrast.Microorganism trapping bait consumption 50ml/ bottle of the present invention, the auspicious special fruit bat attractant consumption 50ml/ bottle of fruit, methyl eugenol consumption 3ml/ bottle.
The above-mentioned a kind of attractant of each plastic bottle, screws bottle cap, and uniform 4 holes on the bottle wall apart from liquid level 2cm in bottle, the aperture in described each hole is 5mm.Carry out field adaptability noon on May 16th, 2015, plastic bottle is hung on from 0.5-1.5 rice, oranges and tangerines ground.Lure dacus dorsalis quantity (head) in table 1 two days later.Visible, microorganism trapping bait of the present invention lures borer population amount all higher than the auspicious special fruit bat attractant of fruit and methyl eugenol, and female worm rate is up to 51.67%.Microorganism trapping bait of the present invention lures the auspicious special fruit bat attractant of worm number ratio fruit to increase by 23.71%, increases by 18.81% than methyl eugenol.The female worm that microorganism trapping bait of the present invention lures than the auspicious special fruit bat attractant of fruit increases by 37.78%, and the female worm lured than methyl eugenol increases by 30 times.
Table 1 microorganism trapping bait of the present invention is to dacus dorsalis trapping experiment
Embodiment 4 microorganism trapping bait of the present invention is to citrus fruit trypetid trapping experiments
Fruit fly trapping experiments is carried out, test reagent: microorganism trapping bait of the present invention on farm, Nanxi, Hekou County, Yunnan Province.Plastic bottle and the operation of installed reagents are identical with embodiment 3, microorganism trapping bait consumption 50ml/ bottle of the present invention, hang on from 0.5-1.5 rice, oranges and tangerines ground in plastic bottle noon on July 5th, 2014 reagent being housed, worm amount within latter 1 day, 2 days and 3 days, is investigated in each bottle in table 2 from feeding intake, visible, trapping bait fermentation of the present invention produces the protein-based compound that waits of volatility and lures fruit fly to eat, Be very effective, and male and female are with luring.
The effect of table 2 microorganism of the present invention trapping bait trapping fruit fly (citrus fruit fly, little trypetid, Bactrocera correcta, melon trypetid etc.)
Embodiment 5 microorganism trapping bait of the present invention is to the comparative trial of citrus fruit fly control efficiency
In the citrus orchard of Mengzi County, Yunnan Province, carry out the test of trapping citrus fruit fly, contrast with methyl eugenol simultaneously.Microorganism trapping bait consumption 50ml/ bottle of the present invention, methyl eugenol consumption 50ml/ bottle.
The plastic bottle and the operation that fill each reagent are identical with embodiment 3, noon on December 4th, 2014, the plastic bottle that reagent is housed is hung on from 0.5-1.5 rice, oranges and tangerines ground, worm amount within latter 1 day, 2 days and 3 days, is investigated in each bottle in table 3 from feeding intake, visible, producing the protein-based compound that waits of volatility with microorganism trapping bait fermentation of the present invention lures citrus fruit fly to eat, except first day lures worm amount lower than except gyplure methyl eugenol, At All Other Times, the effect of the large trypetid of trapping is all better than gyplure methyl eugenol, and male and female are with luring.In three days, microorganism trapping bait trapping citrus fruit fly number ratio methyl eugenol of the present invention increases by 64.43%, and the citrus fruit fly female worm number ratio methyl eugenol of trapping increases by 33.25 times.
The effect of table 3 microorganism trapping bait trapping of the present invention citrus fruit fly
Embodiment 6 microorganism of the present invention trapping bait and yellow plate field lure worm comparative trial
Trap effect test is carried out in tomato vegetable base, Mengzi County, Yunnan Province, contrasts with field and the yellow plate of 30 × 30cm.The bottled 50ml of each yellow plastic microorganism trapping bait of the present invention, screws bottle cap, and uniform 4 holes on the bottle wall apart from liquid level 2cm place in bottle, the aperture in described each hole is 5mm.Carry out field adaptability noon on June 16th, 2015, hung on by plastic bottle apart from 0.5 ~ 0.8 meter, ground high, every mu is hung plastic bottle 8, adjacent plastic bottle interval 5 ~ 10m; In same field, Jiang Huangban hangs on apart from ground 0.5-0.8 rice high, and every mu is hung yellow plate 8, adjacent yellow plate interval 5 ~ 10m.Often lure Americal rice leaf miner quantity (head) in table 4 two days later.Visible, microorganism trapping bait of the present invention lures borer population amount all higher than yellow plate average every day, and in 8 days, microorganism trapping bait of the present invention lures worm 1988, yellow trap 379, is 5.2 times of yellow trap amount.
The comparative trial that table 4 microorganism of the present invention trapping bait and yellow plate are lured Americal rice leaf miner
Implement 7 microorganism trapping baits of the present invention to Americal rice leaf miner male and female quantity trapping experiments
In Niu Mo village, Jiangchuan County, Yunnan Province, Vegetable Base carries out cowpea Americal rice leaf miner trapping experiments, microorganism trapping bait of the present invention for 50ml is directly added outer wall to be posted in yellow adhesive tape plastic bottle, screw bottle cap, uniform 4 holes on the bottle wall apart from liquid level 2cm place in bottle, the aperture in described each hole is 5mm.Distance 0.5 ~ 0.8 meter, ground is hung on high noon on February 3rd, 2015, worm amount within latter 1 day, 2 days and 3 days, is investigated in each bottle in table 5 from feeding intake, visible, the protein-based compound that waits of the volatility produced with microorganism trapping bait fermentation of the present invention lures Americal rice leaf miner to eat, Be very effective, and male and female are with luring, female worm rate reaches 55 ~ 60%.
Table 5 microorganism trapping bait of the present invention catches male and female Americal rice leaf miner statistics
Claims (5)
1. a microorganism trapping bait, is characterized in that: its active component is the zymotic fluid of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722.
2. a kind of microorganism trapping bait according to claim 1, is characterized in that, the zymotic fluid of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 prepares by the following method:
(1) by 5 ~ 10% inoculum concentrations, described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 of activation is inoculated into seed culture medium, under cultivation temperature 35 DEG C, rotating speed 120rpm/min condition, 36 ~ 48h cultivated by shaking table, obtain primary seed solution, described seed culture medium is LB liquid medium, pH7.0 ~ 7.2;
(2) by 10 ~ 12% inoculum concentrations, primary seed solution is transferred to the secondary seed medium in seeding tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, throughput is 1000L/h, and tank pressure cultivates 36 ~ 48h under remaining on 0.02 ~ 0.03MPa condition, obtains secondary seed solution; Described secondary seed medium is grouped into by the following one-tenth of following mass fraction: corn flour 0.3 ~ 0.5%, analysis for soybean powder 1 ~ 1.5%, sucrose 0.2 ~ 0.5%, peptone 0.1 ~ 0.3%, fish meal 0.1 ~ 0.3%, MgSO
47H
2o0.02 ~ 0.04%, CaCO
30.4 ~ 0.8%, (NH
4)
2sO
40.03 ~ 0.06%, surplus is water, pH7.0 ~ 7.2;
(3) by 10 ~ 12% inoculum concentrations, secondary seed solution is inoculated into the fermentation medium in fermentation tank, at 28 ~ 37 DEG C, rotating speed 100rpm/min, throughput is 10000L/h, tank pressure cultivates 48 ~ 72h under remaining on 0.02 ~ 0.03MPa condition, You effect Jun Shuo≤3 × 10 alive of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 in the zymotic fluid after cultivation
9during cfu/ml, this zymotic fluid is the zymotic fluid of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722, and described fermentation medium is identical with step (2) described secondary seed medium.
3. the microorganism trapping bait described in claim 1 or 2 is in the application of control Fruit fly.
4. the microorganism trapping bait described in claim 1 or 2 is in the application of control Americal rice leaf miner.
5. application according to claim 3, it is characterized in that: described application is loaded in plastic bottle by described microorganism trapping bait, and the charge weight of every bottle is 50 ~ 100mL, screws bottle cap, uniform 4 holes on the bottle wall apart from liquid level 1 ~ 3cm in bottle, the aperture in described each hole is 4 ~ 6mm; Plastic bottle is hung on overhead 0.5 ~ 1.5 meter of, every mu is hung plastic bottle 8 ~ 10, adjacent plastic bottle interval 5 ~ 7m; In plastic bottle, described microorganism trapping bait 25 ~ 50mL is added once every 20 ~ 30 days.
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| CN105410032B (en) | 2018-04-24 |
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Denomination of invention: A Microbial Trapping Agent and Its Application Effective date of registration: 20231120 Granted publication date: 20180424 Pledgee: Kunming Dongfeng Sub branch of Bank of China Ltd. Pledgor: MICROBIAL FERMENTATION ENGINEERING RESEARCH CENTER Co.,Ltd. OF YUNNAN PROVINCE Registration number: Y2023530000066 |