AU2020102672A4 - Plant attractant composition for tephritidae pests and preparation method thereof - Google Patents

Plant attractant composition for tephritidae pests and preparation method thereof Download PDF

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AU2020102672A4
AU2020102672A4 AU2020102672A AU2020102672A AU2020102672A4 AU 2020102672 A4 AU2020102672 A4 AU 2020102672A4 AU 2020102672 A AU2020102672 A AU 2020102672A AU 2020102672 A AU2020102672 A AU 2020102672A AU 2020102672 A4 AU2020102672 A4 AU 2020102672A4
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Prior art keywords
powder
clove
acorus
muell
extract
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AU2020102672A
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Xuyuan Gao
Xiaodong Jiang
Xiuzhen Long
Dewei Wei
Yonghao Yu
Xianru Zeng
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Guangxi Qifeng Juzhou Fruit Industry Co Ltd
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Guangxi Qifeng Juzhou Fruit Industry Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/002Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/28Myrtaceae [Myrtle family], e.g. teatree or clove

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Austracy The present invention belongs to the field of agricultural agents and particularly provides a plant attractant composition for Tephritidae pests and a preparation method thereof. The plant attractant composition includes the following major ingredients: Melaleuca bracteata F. Muell., Asarum sieboldii, Acorus tatarinowii and clove. The present invention adopts natural plant ingredients, is nontoxic, pollution-free, and safe to people, livestock and the environment, can attract multiple Tephritidae pests including male pests and female pests, and has obvious effects. 1

Description

Austracy
The present invention belongs to the field of agricultural agents and particularly provides a plant attractant composition for Tephritidae pests and a preparation method thereof. The plant attractant composition includes the following major ingredients: Melaleuca bracteata F. Muell., Asarum sieboldii, Acorus tatarinowii and clove. The present invention adopts natural plant ingredients, is nontoxic, pollution-free, and safe to people, livestock and the environment, can attract multiple Tephritidaepests including male pests and female pests, and has obvious effects.
Description
PLANT ATTRACTANT COMPOSITION FOR TEPHRITIDAE PESTS AND PREPARATION METHOD THEREOF
Technical Field
The present invention belongs to the field of agricultural agents and particularly provides a plant attractant composition for Tephritidaepests and a preparation method thereof.
Background
Tephritidae insects are one of the most important Diptera economic groups, including over 250 species, and are harmful to commercial crops including fruits and vegetables. The fruits and melon vegetables are particularly seriously harmed by some Tephritidae species such as Bactrocera dorsalis Hendel, Bactrocera cucurbitae, Bactrocera tau, Ceratitis capitata, Rhagoletis pomonella, Anastrepha ludens and Rhagoletis cingulata. These species become important economic pests in tropical and subtropical areas. The Bactrocera dorsalis Hendel is a major Tephritidaespecies. The Bactrocera dorsalis Hendel belongs to Diptera Tephritidae fruit flies. It is also known as oriental fruit fly, is a serious pest in the fruit tree planting industry in Asian-Pacific region, and is also a risky harmful organism for strict quarantine and prevention in dozens of countries and regions in the world. The Bactrocera dorsalis Hendel has an extensive host range and may harm more than 250 kinds of fruits and vegetables in 46 families. Since the mid-1990s, the Bactrocera dorsalis Hendel has extended from several provinces and regions in southern China to the Southern China, Eastern China and Southwest Region and caused large-area outbreaks, thereby seriously affecting safe fruit production and export trade in the southern China. At present, the Bactrocera dorsalis Hendel is mainly controlled in the
Description
following manners: chemical pesticide control, attractant killing control and fruit bagging. The attractant killing control includes male killing by sex attractants and male and female killing control by food attractants. Environmental pollution, pesticide residues and other problems are often caused by the chemical pesticide control. Control of the Tephritidae pests is developing towards a direction of greening and environmental protection and decreased pesticide application in the future. The present invention provides a novel plant attractant composition for Tephritidae pests, is nontoxic and pollution-free to people, livestock and the environment, and has the characteristics of green and environmental protection.
Summary
A purpose of the present invention is to overcome the above defects and provide an efficient plant attractant composition for Tephritidae pests and a preparation method thereof. The plant attractant composition is nontoxic and pollution-free to people, livestock and the environment, green and environment-friendly. Technical solutions of the present invention are as follows: the plant attractant composition includes the following major ingredients: Melaleuca bracteata F. Muell., Asarum sieboldii, Acorus tatarinowiiand clove. Various major ingredients of the trapping bag are natural plant ingredients, do not belong to synthetic agents, and have obvious attractive effects. Each major ingredient has an attractive effect alone, and a combination effect is excellent. Preferably, the plant attractant composition for Tephritidae pests further includes excipients. The excipients are one or more of banana meal, pumpkin powder, yeast powder, brown powdered sugar and sodium diacetate; and a mass percent of the excipients is 0-45%. Preferably, the plant attractant composition includes the following major ingredients in percentage by mass: 0.1-91% of Melaleuca bracteata F. Muell.,
Description
0.1-90% of Asarum sieboldii, 0.1-90% of Acorus tatarinowii and 0.1-90% of clove. Preferably, the plant attractant composition includes the following major ingredients in percentage by mass: 14-60% of Melaleuca bracteata F. Muell., -50% of Asarum sieboldii, 10-40% of Acorus tatarinowiiand 10-40% of clove. Preferably, each major ingredient is one of powder, extract and essential oil or a mixture of several. The present invention further discloses a preparation method of the plant attractant composition for Tephritidae pests. The preparation method includes the following steps: step Si: preparing major ingredients: Melaleuca bracteataF. Muell.: drying and crushing leaves; sieving the leaves by a 40-mesh sieve to obtain powdered Melaleuca bracteata F. Muell.; extractng the powdered Melaleuca bracteata F. Muell. or dried leaves with an organic solvent to obtain Melaleuca bracteataF. Muell. extract; distilling fresh leaves with water vapor to obtain Melaleuca bracteataF. Muell. essential oil, so as to prepare Melaleuca bracteataF. Muell. powder, extract and essential oil for later use; Asarum sieboldii: drying and crushing Asarum sieboldii roots; sieving the roots by the 40-mesh sieve to obtain Asarum sieboldii powder; extracting the Asarum sieboldii powder or dried roots with an organic solvent to obtain Asarum sieboldii extract; distilling fresh Asarum sieboldii roots with water vapor to obtain Asarum sieboldii essential oil, so as to prepare Asarum sieboldii powder, extract and essential oil for later use; Acorus tatarinowii: drying and crushing Acorus tatarinowiiroots; sieving the roots by the 40-mesh sieve to obtain Acorus tatarinowii powder; extracting the Acorus tatarinowiipowder or dried roots with an organic solvent to obtain Acorus tatarinowii extract; distilling fresh Acorus tatarinowii roots with water vapor to obtain Acorus tatarinowiiessential oil, so as to prepare Acorus tatarinowiipowder, extract and essential oil for later use;
Description
clove: drying and crushing clove flowers or fruits; sieving the clove flowers or fruits by the 40-mesh sieve to obtain clove powder; extracting the clove powder or dried flowers or fruits with an organic solvent to obtain clove extract; distilling fresh flowers or fruits with water vapor to obtain clove essential oil, so as to prepare clove powder, extract and essential oil for later use; step S2: mixing the various major ingredients prepared in the step Sl in percentage by mass, and adding excipients such as banana meal, pumpkin powder, yeast powder, brown powdered sugar and sodium diacetate. During mixing, the various major ingredients may be combined in one or more states such as the powder, extract and essential oil. Compared with the prior art, the present invention has beneficial effects as follows: 1. The plant attractant composition adopts the natural plant ingredients, and is nontoxic, pollution-free, and safe to people, livestock and the environment. 2. The plant attractant composition can attract multiple Tephritidae pests including male pests and female pests and has obvious effects. 3. The plant attractant composition is simple in preparation, diversified in use, and easy to use.
Detailed Description
The present invention will be further described below in combination with specific embodiments. The present invention is described by taking Clausena lansium that will ripen soon as an example and taking a commercially available fruit fly attractant as a control group. 1. Preparation of a plant attractant composition for Tephritidaepests 3 parts of 100 g of an attractant composition were prepared; the first part of the attractant composition includes the following ingredients: 10 g of Melaleuca
A
Description
bracteataF. Muell. extract, 1 g of Melaleuca bracteataF. Muell. essential oil, 14 g of Asarum sieboldii extract, 2 g of Asarum sieboldii essential oil, 18 g of Acorus tatarinowii extract, 10 g of clove powder, 5 g of banana meal, 15 g of pumpkin powder, 15 g of yeast powder, 9 g of brown powdered sugar and 1 g of sodium diacetate. The second part of the attractant composition includes the following ingredients: 20 g of Melaleuca bracteata F. Muell. essential oil, 25 g of Asarum sieboldii essential oil, 20 g of Acorus tatarinowiiextract, 30 g of clove essential oil, 1 g of banana meal, 1 g of pumpkin powder, 1 g of yeast powder, 1 g of brown powdered sugar and 1 g of sodium diacetate. The third part of the attractant composition includes the following ingredients: g of Melaleuca bracteataF. Muell. extract, 8 g of Asarum sieboldii extract, 16 g of Acorus tatarinowiiextract, 6 g of clove powder and 10 g of yeast powder. 2. Agent dosing During use, water or insecticide liquid in an amount of 3-5 times that of the volume was added into the attractant composition to be uniformly mixed with the attractant composition so as to trap Tephritidae. The formula is applicable to trapping the Tephritidae by a trapper or in an open container, and multiple female and male Tephritidae on fruit and vegetable crop may be attracted. Population density of Tephritidae pests in the field is greatly decreased during continuous application, thereby decreasing the dosage of chemical pesticides and lowering the degree of environmental pollution. 3. Field use conditions: (1) Field use conditions of the first part of the attractant composition A test crop is the Clausena lansium that will ripen soon, and carambola, mango, pumpkin and loofah are planted nearby the Clausena lansium. During test, ml of the first part of the attractant composition with the insecticide liquid was added into the bottom of a Tephritidaetrapper bottle; and 1 ml of a commercially
Description
available Tephritidae attractant was added into the bottom of a control trapper bottle. The two attractants were hung on Clausena lansium trees; the trapper bottle was 1.2 m away from the ground; spacing between every two trapper bottles was m; and the bottles were gotten back for investigation and statistics and the Tephritidaewas removed and put back every 5 days, and investigation was totally conducted for 15 days. The results were as shown in Table 1. Table 1 Conditions of attracted insects by different attractants in Clausena lansium orchard
Treatment Tephritidae species Number of Number of Number of
insects within insects within insects
the first 5 days 6-10 days within
(head) (head) 11-15 days
(head)
Attractant Male Bactrocera dorsalisHendel 65 51 74
composition in the Female Bactroceradorsalis 16 7 20
present invention Hendel
Male Bactrocera cucurbitae 1 5 4
Female Bactroceracucurbitae 5 5 1
Male Bactrocera tau 4 1 5
Female Bactrocera tau 6 3 5
Male Bactrocerascutellata 1 3 1
Female Bactrocerascutellata 2 1 1
Total 100 76 111
Commercially Male Bactrocera dorsalisHendel 102 63 92
available Female Bactroceradorsalis 0 0 0
Tephritidae Hendel
attractant Male Bactrocera cucurbitae 0 0 0
Female Bactroceracucurbitae 0 0 0
Male Bactrocera tau 0 0 0
Description
Female Bactrocera tau 0 0 0
Male Bactrocerascutellata 0 0 0
Female Bactrocerascutellata 0 0 0
Total 102 63 92
Through preliminary test results, 4 Tephritidae species and partial male Tephritidae insects may be attracted by the attractant composition in the present invention, while only the male Bactrocera dorsalis Hendel is attracted by the commercially available Tephritidae attractant. In addition, the total number of the Tephritidae insects attracted by the attractant composition in the present invention within 10 days and 15 days is more than that of the commercially available Tephritidae attractant, and the attractant composition in the present invention has an obvious attractive effect. (2) Field use conditions of the second part of the attractant composition A test crop is mango that will ripen soon, and guava, bitter gourd, pumpkin and loofah are planted nearby the mango. During test, 50 ml of the second part of the attractant composition with the insecticide liquid was added into the bottom of a Tephritidae trapper bottle; and 1 ml of the commercially available Tephritidae attractant was added into the bottom of a control trapper bottle. The two attractants were hung on mango trees; the trapper bottle was 1.5 m away from the ground; spacing between every two trapper bottles was 20 m; and the bottles were gotten back for investigation and statistics and the Tephritidaewas removed and put back every 5 days, and investigation was totally conducted for 15 days. The results were as shown in Table 2. Table 2 Conditions of attracted insects by different attractants in mango orchard
Treatment Tephritidaespecies Number of Number of Number of
insects within insects within insects
the first 5 days 6-10 days within
'7
Description
(head) (head) 11-15 days
(head)
Attractant Male BactroceradorsalisHendel 254 190 156
composition in the Female Bactroceradorsalis 6 2 1
present invention Hendel
Male Bactroceracucurbitae 2 7 2
Female Bactrocera cucurbitae 1 1 1
Male Bactroceratau 1 1 1
Female Bactrocera tau 1 1 2
Total 265 202 163
Commercially Male BactroceradorsalisHendel 213 190 156
available Female Bactroceradorsalis 0 0 0
Tephritidae Hendel
attractant Male Bactroceracucurbitae 0 0 0
Female Bactrocera cucurbitae 0 0 0
Male Bactroceratau 0 0 0
Female Bactrocera tau 0 0 0
Total 213 190 156
Through the test results, 3 Tephritidae species and partial male Tephritidae insects may be attracted by the attractant composition in the present invention, while only the male Bactrocera dorsalis Hendel is attracted by the commercially available Tephritidae attractant. In addition, the total species and number of the Tephritidae insects attracted by the composition in the present invention are larger than those of the commercially available Tephritidaeattractant. (3) Field use conditions of the third part of the attractant composition A test crop is guava at a maturation stage, and bitter gourd is planted nearby the guava. During test, 50 ml of the third part of the attractant composition with the
Q
Description
insecticide liquid was added into the bottom of a Tephritidae trapper bottle; and 1 ml of the commercially available Tephritidae attractant was added into the bottom of a control trapper bottle. The two attractants were hung on guava trees; the trapper bottle was 1.5 m away from the ground; spacing between every two trapper bottles was 20 m; and the bottles were gotten back for investigation and statistics and the Tephritidaewas removed and put back every 5 days, and investigation was totally conducted for 15 days. The results were as shown in Table 3. Table 3 Conditions of attracted insects by different attractants in guava orchard
Treatment Tephritidae species Number of Number of Number of
insects within insects within insects within
the first 5 days 6-10 days 11-15 days
(head) (head) (head)
Attractant Male BactroceradorsalisHendel 255 200 220
composition in the Female Bactrocera dorsalis 1 1 2
present invention Hendel
Male Bactrocera cucurbitae 1 3 1
Female Bactroceracucurbitae 1 2 1
Total 258 206 224
Commercially Male BactroceradorsalisHendel 225 204 203
available Female Bactrocera dorsalis 0 0 0
Tephritidae Hendel
attractant Male Bactrocera cucurbitae 0 0 0
Female Bactroceracucurbitae 0 0 0
Total 225 204 203
Through the test results, lots of the male Bactrocera dorsalis Hendel, some female Bactrocera dorsalis Hendel and a small amount of the male and female Bactroceracucurbitae may be attracted by the attractant composition in the present invention, while only the male Bactrocera dorsalis Hendel is attracted by the
Description
commercially available Tephritidaeattractant. The total species and number of the attracted Tephritidae insects in the solution of the present invention are larger than those of the commercially available Tephritidae attractant. It should be understood that, these embodiments are merely used for describing the present invention, rather than limiting the scope of the present invention. In addition, it should be understood that, after the contents disclosed by the present invention are read, various modifications or changes may be made to the present invention by those skilled in the art. These equivalent forms shall also be included in the scope defined by claims of the present invention.
1 +

Claims (5)

Claims
1. A plant attractant composition for Tephritidae pests, comprising the following major ingredients: Melaleuca bracteata F. Muell., Asarum sieboldii, Acorus tatarinowiiand clove, wherein the plant attractant composition comprises the following major ingredients in percentage by mass: 0.1-91% of Melaleuca bracteata F. Muell., 0.1-90% of Asarum sieboldii, 0.1-90% of Acorus tatarinowiiand 0.1-90% of clove; the plant attractant composition also comprises excipients: a mass percent of the excipients is 0-45%.
2. The plant attractant composition for Tephritidaepests according to claim 1, further comprising excipients, wherein the excipients are one or more of banana meal, pumpkin powder, yeast powder, brown powdered sugar and sodium diacetate.
3. The plant attractant composition for Tephritidaepests according to claim 1, comprising the following major ingredients in percentage by mass: 14-60% of Melaleuca bracteata F. Muell., 10-50% of Asarum sieboldii, 10-40% of Acorus tatarinowiiand 10-40% of clove.
4. The plant attractant composition for Tephritidaepests according to claim 1, wherein each major ingredient is one of powder, extract and essential oil or a mixture of several.
5. A preparation method of the plant attractant composition for Tephritidae pests of any one of claims 1-4, comprising the following steps: step Si: preparing major ingredients: Melaleuca bracteataF. Muell.: drying and crushing leaves; sieving the leaves by a 40-mesh sieve to obtain powdered Melaleuca bracteata F. Muell.; extractng the powdered Melaleuca bracteata F. Muell. or dried leaves with an organic solvent to obtain Melaleuca bracteataF. Muell. extract; distilling fresh leaves with water vapor to obtain Melaleuca bracteataF. Muell. essential oil, so as to prepare Melaleuca bracteataF. Muell. powder, extract and essential oil for later use; Asarum sieboldii: drying and crushing Asarum sieboldii roots; sieving the roots by the 40-mesh sieve to obtain Asarum sieboldii powder; extracting the
Claims
Asarum sieboldii powder or dried roots with an organic solvent to obtain Asarum sieboldii extract; distilling fresh Asarum sieboldii roots with water vapor to obtain Asarum sieboldii essential oil, so as to prepare Asarum sieboldii powder, extract and essential oil for later use; Acorus tatarinowii: drying and crushing Acorus tatarinowiiroots; sieving the roots by the 40-mesh sieve to obtain Acorus tatarinowii powder; extracting the Acorus tatarinowiipowder or dried roots with an organic solvent to obtain Acorus tatarinowii extract; distilling fresh Acorus tatarinowii roots with water vapor to obtain Acorus tatarinowiiessential oil, so as to prepare Acorus tatarinowiipowder, extract and essential oil for later use; clove: drying and crushing clove flowers or fruits; sieving the clove flowers or fruits by the 40-mesh sieve to obtain clove powder; extracting the clove powder or dried flowers or fruits with an organic solvent to obtain clove extract; distilling fresh flowers or fruits with water vapor to obtain clove essential oil, so as to prepare clove powder, extract and essential oil for later use; step S2: mixing the various major ingredients prepared in the step Si in percentage by mass, and adding excipients such as banana meal, pumpkin powder, yeast powder, brown powdered sugar and sodium diacetate.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115553293A (en) * 2022-11-14 2023-01-03 广东省农业科学院植物保护研究所 Citrus fruit fly repellent and preparation method thereof
CN116004433A (en) * 2022-09-16 2023-04-25 华南农业大学 Citrobacter malonate-free and application thereof in attracting bactrocera dorsalis

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116004433A (en) * 2022-09-16 2023-04-25 华南农业大学 Citrobacter malonate-free and application thereof in attracting bactrocera dorsalis
CN116004433B (en) * 2022-09-16 2024-02-20 华南农业大学 Citrobacter malonate-free and application thereof in attracting bactrocera dorsalis
CN115553293A (en) * 2022-11-14 2023-01-03 广东省农业科学院植物保护研究所 Citrus fruit fly repellent and preparation method thereof

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