CN107287139A - A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved - Google Patents
A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to microbial agriculture technical field, more particularly to a kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved.The invention provides a kind of microorganism formulation and preparation method and application, the microorganism formulation is made using fermentation of bacillus subtilis, shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate and composite fertilizer in the nutrient solution of fermentation, so that said preparation can not only play a part of suppressing harmful bacteria, additionally it is possible to provide substantial amounts of nutriment for crop.Experiment shows, microbial inoculum is made to the inhibition zones of pathogenic bacteria up to more than 6 times of control group in the microorganism formulation that the present invention is provided.Field experiment shows that the microbial inoculum has significant raising to the micronutrient levels in soil, improves the diseases such as the ginseng caused by continuous cropping, pseudo-ginseng, glutinous rehmannia.
Description
Technical field
The present invention relates to microbial manure technical field, more particularly to a kind of microorganism formulation and preparation method thereof is with changing
Application in kind continuous cropping obstacle.
Background technology
Agricultural chemicals, chemical fertilizer are the essential material used for agriculture of modern agriculture, are meeting the same of the demand that people increase to grain
When, also pay high ecological environment and Resources Consumption.Such as chemical fertilizer and chemical pesticide have chemical fixation in soil,
Overuse can cause the adverse consequences such as soil hardening, soil acidity increase, meanwhile, necessarily cause earth's surface and underground water pollution
Exacerbation, polluted by nutriments such as nitrogen, phosphorus more than China's surface water body.It is a large amount of to also result in many using chemical fertilizer, agricultural chemicals
Biotic population quantity is drastically reduced, or even extinction, and the heavy damage ecological balance, long term frequent using chemical pesticide can cause disease
There is the resistance to the action of a drug in bacterium, insect pest, the drug effect of chemical pesticide in itself is gradually reduced, to reach that sterilization purpose just needs to increase dosage.
So, agricultural cost can be not only improved, also persticide residue is increased sharply, it is very unfavorable to health, and severe contamination environment.
After same crop or nearly edge continuous cropping, in the case of normal management, yield reduction, product can be also produced
The phenomenon that qualitative change is bad, bearing status is deteriorated, here it is continuous cropping obstacle.Traditional theory thinks, main the reason for continuous cropping obstacles
There are disease caused by three broad aspects, i.e. edaphon, rhizosphere allelopathic Auto toxicity and soil nutrient not enough.Also some theories are recognized
To be the deterioration of soil physical and chemical property, such as soil acidification is hardened and gas permeability declines.And current solution is mostly all by mesh
Light is concentrated on microorganism disease, though it is effective unsatisfactory.In addition, influenceing be one from poison and pathogenic microorganism
Short-term effect, the suppression harmful bacteria secreted in Ginseng Growth should be able to natural degradation within a short period of time；Edaphon
Flora can be also restored by Natural regulation within a certain period of time.
People are after gradually understanding above mentioned problem, and microorganism medicine fertilizer arises at the historic moment.Microorganism medicine fertilizer is the life with microorganism
Life activity causes crop to obtain specific fertilizer effect and/or prevents and treats a kind of living microorganisms product of pest and disease damage effect.Microorganism
Contain a large amount of beneficial microbes in medicine fertilizer, crop nutritional condition can be improved, some invalid states in nitrogen and activating soil are fixed
Nutrient, create good soil microenvironment to promote the growth of crop, while the pest and disease damage of plant can be played
To inhibitory action.It is free from environmental pollution to person poultry harmless also, microorganism medicine fertilizer is harmless to pest natural enemy.
The microorganism medicine fertilizer of current development and production is remained in many weak points.Its unreasonable, processing mode of formula
Mixed mostly using finished product microbial inoculum with filler, lack fermentating metabolism product, had a strong impact on effect of microbial manure.
The content of the invention
In view of this, the technical problem to be solved in the present invention be to provide a kind of microorganism formulation and preparation method thereof with
Improve the application in continuous cropping obstacle, the microorganism formulation can play good inhibitory action to phytopathogen, improve soil
Nutrition and quality, and continuous cropping obstacles can be improved.
The microorganism formulation that the present invention is provided, by strain, shrimp and crab shells, collagen, sodium chloride, compound amino acid, carbon water
Compound and composite fertilizer are made；The strain includes bacillus subtilis；
Strain, shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate, composite fertilizer mass ratio for (0.1~
1)：(0.1~10)：(0.01~1)：(0.01~9)：(1~50)：(1~20)：(1~10).
The present invention selection bacillus subtilis compounded with other bacteriums, cooperation shrimp and crab shells, collagen, sodium chloride,
Amino acid, carbohydrate, composite fertilizer are fermented, and there is substantial amounts of viable bacteria in obtained biological agent.Preferably, this
Viable count in the microorganism formulation provided is provided and is not less than 1 × 109Individual/mL~20 × 109Individual/mL.It is preferred that, microorganism formulation
Middle viable count is 1 × 1010Individual/mL.And, substantial amounts of organic matter, middle trace element are contained in the microorganism formulation that the present invention is provided,
So as to recover soil fertility and take into account killing pathogenic microorganisms, effectively regulation soil micro-ecosystem constitutes, optimizes soil nutrient
Composition, the bioactivity for improving soil, improve continuous cropping obstacles.
The strain also includes bacillus thuringiensis, bacillus licheniformis, bacillus megaterium；The bacillus subtilis
The quantity ratio of bacterium and bacillus thuringiensis, bacillus licheniformis, bacillus megaterium is (0.5~5)：(0.5~5)：(0.5
~5)：(0.5~5).
Bacillus subtilis (Bacillus subtilis), often uses it for sewage disposal, aquaculture in the prior art
Field.Research shows that it can produce subtilin, polymyxins, nystatin, gramicidins isoreactivity material, to caused by soil
Germ inhibitory action is strong.The bacillus subtilis strain used in the present invention is in the management of Chinese agriculture Microbiological Culture Collection
The heart, numbering is ACCC No.10242.
Bacillus thuringiensis (Bacillus thuringiensis), can produce two big toxoids, i.e. endotoxin (companion cell
Crystal) and exotoxin, make insect stop taking food, last insect is because hungry and dead.The golden gemma bars of Su Yun used in the present invention
Bacteria strain comes from Chinese agriculture Microbiological Culture Collection administrative center, and numbering is ACCC No.01599.
Bacillus licheniformis (Bacilluslicheniformis) can produce resistant activity material, and with unique biology
Take oxygen mechanism of action by force, the growth and breeding of pathogenic bacteria can be suppressed, also with fixed nitrogen or crop rhizosphere growth-promoting ability.Used in the present invention
Lichem bacillus strain come from Chinese agriculture Microbiological Culture Collection administrative center, numbering is ACCC No.02975.
Bacillus megaterium (Bacillus megaterium), is saprophytic bacteria in common oil, industrially for producing
Glucose isomerase, while being also the decomposer of organophosphor, therefore, agriculturally can be used for manufacture phosphorous bacterial fertilizer.The present invention
The bacillus megaterium bacterial strain of middle use comes from Chinese agriculture Microbiological Culture Collection administrative center, and numbering is ACCC
No.04314。
In some embodiments, microorganism formulation is made by the raw material of following mass parts：
0.1 part of bacillus subtilis, 1 part of shrimp med, 0.01 part of collagen, 9 parts of NaCl, 1 part of compound amino acid, carbon water
20 parts of compound, 1 part of composite fertilizer.
In some embodiments, microorganism formulation is made by the raw material of following mass parts：
1 part of bacillus subtilis, 0.1 part of crab meat, 1 part of collagen, 0.01 part of NaCl, 50 parts of compound amino acid, carbon water
1 part of compound, 10 parts of composite fertilizer.
In some embodiments, microorganism formulation is made by the raw material of following mass parts：
0.5 part of bacillus subtilis, 0.5 part of bacillus thuringiensis, 0.5 part of bacillus licheniformis, bacillus megaterium
0.5 part, 0.5 part of crab meat, 0.05 part of collagen, 0.9 part of NaCl, 2 parts of compound amino acid, 3 parts of carbohydrate, composite fertilizer 3
Part.
In some embodiments, microorganism formulation is made by the raw material of following mass parts：
0.3 part of bacillus subtilis, 0.2 part of bacillus thuringiensis, 1 part of bacillus licheniformis, bacillus megaterium 0.3
Part, 1 part of shrimp crab powder, 0.05 part of collagen, 0.05 part of NaCl, 10 parts of compound amino acid, 20 parts of carbohydrate, composite fertilizer
10 parts.
Amino acid (amino acid) is important nutriment, the main component of present invention compound amino acid used be containing
Glycine, alanine, leucine, isoleucine, valine, cystine, cysteine, methionine, threonine, serine,
Phenylalanine, tyrosine, tryptophan, proline, hydroxyproline, glutamic acid, asparatate etc..Its source is not limited, can be with
It is animal or plant acidolysis, alkaline hydrolysis is obtained or microbial fermentation is made.In the present invention, the compound amino acid by
Sea-tangle zymotic fluid, monohydrate zinc sulphate and four hydration ammonium heptamolybdate compositions, wherein adding one in per 80mL~120mL sea-tangle zymotic fluids
The hydration ammonium heptamolybdates of water zinc sulphate 20g~40g and four 1g~3g.
The sea-tangle zymotic fluid can be also made by commercially available and obtain through microbial fermentation, enzymolysis.
Carbohydrate (carbohydrate) be energy matter, the present invention in, carbohydrate be selected from glucose, fructose
Or sucrose.
In the present invention, N, P, K mass ratio are (10~20) in composite fertilizer：(10~20)：(10~20).
Wherein N, P, K are that water miscible component is present, such as：NO3-、NH4+、PO3-、PO4 3-、HPO4 2-、H2PO4 -、P6O18 6-、
P2O7 4-、K+。
In some embodiments, the existence form of composite fertilizer's N P and K is NH4NO3/K2HPO4/KH2PO4, N/P/K=15/15/
15th, N/P/K=15/15/17 or N/P/K=15/15/20.
The present invention is not limited the source of shrimp and crab shells, can be that the aquatic of chitin that be rich in that rivers,lakes and seas are arbitrarily originated is moved
Beyond the region of objective existence shell, can also include meat, and heretofore described shrimp and crab shells are the fine grained that dry product crosses the mesh sieve of 20 mesh~100 after crushed
Powder.Preferably shrimp shell meal, crab shell powder or shrimp crab powder.
In the present invention, collagen is the powder that Animal Skin is crushed or extracted, and wherein the content of collagen is 30%~65%.
In some embodiments, collagen is pigskin collagen.In other embodiments, collagen is collagen.Separately
In some embodiments, collagen is cow leather collagen.
The preparation method for the microorganism formulation that the present invention is provided, including：
Step 1：Strain, shrimp and crab shells, collagen, sodium chloride are mixed with water, first is carried out and ferments, nutrient solution is made；
Step 2：Amino acid, carbohydrate, composite fertilizer mix with nutrient solution, carry out second and ferment, microorganism system is made
Agent.
In the present invention, the temperature of the first fermentation is 25 DEG C~35 DEG C；Time is 3 days~5 days；Condition is bubble aeration；The
The temperature of two fermentations is 25 DEG C~35 DEG C；Time is 3 days~5 days；Condition is bubble aeration.
In some embodiments, the temperature of the first fermentation is 28 DEG C~32 DEG C；Time is 3 days；Condition is bubble aeration；Second
The temperature of fermentation is 28 DEG C~32 DEG C；Time is 3 days；Condition is bubble aeration.
Zymotic fluid pH maintains 6.6~7.0.Preferably 7.0.
Blow rate 20L/min, dissolved oxygen amount is 20%.
In the present invention, after being mixed in step 1 with water, the mass fraction of strain is 0.1 ‰~1 ‰；The quality of shrimp and crab shells point
Number is 0.1 ‰~10 ‰；The mass fraction of collagen is 0.01 ‰~1 ‰；The mass fraction of sodium chloride be 0.01 ‰~
9‰；
After being mixed in step 2 with nutrient solution, the mass fraction of amino acid is 1 ‰~50 ‰；The quality of carbohydrate point
Number is 1 ‰~20 ‰；The mass fraction of composite fertilizer is 1 ‰~10 ‰.
Water described in step 1 is domestic water.
The microorganism formulation that the present invention is provided is in suppressing phytopathogen, improving soil property, improvement continuous cropping obstacles
Using.
In the embodiment of the present invention, the phytopathogen is Fusarium oxysporum, epidemic disease bacterium etc.；The crop is ginseng, three
Seven or glutinous rehmannia.
The microorganism formulation that the present invention is provided is verified by experiments, with very high cell density, with certain sterilization,
Bacteriostasis, and the content of Trace Elements in Soil can be increased, improve crop incidence of disease rise phenomenon caused by continuous cropping.It is preferred that
, suppression phytopathogen of the present invention refers to suppress Fusarium oxysporum；Improvement continuous cropping obstacles of the present invention are to change
The continuous cropping obstacle of philanthropist's ginseng；The soil property of the present invention that improves refers to improve the micronutrient levels in soil.
The microbial bacterial agent that the present invention is provided can be used separately as microbial bacterial agent, also further can be diluted or be compounded
Microbial bacterial agent is made after natural surfactant.The microbial bacterial agent that the present invention is provided includes the microorganism system that the present invention is provided
Agent and surfactant.In some embodiments, microbial bacterial agent includes 1 part of microorganism formulation；0.001 part of surfactant~
0.02 part；1 part~10 parts of water.
The surfactant is selected from soapberry extract, gleditsia sinensis extract solution or glycine betaine.
Soapberry extract of the present invention is obtains through water extraction, and its preparation method is：By soapberry kind skin dries pulverizing,
Extracting in water, 60 DEG C are flowed back 2 hours, are extracted 3 times, and filtering is concentrated after the extract solution after multiple times of filtration is well mixed, obtained
The soapberry extract after must concentrating.Extract solution is 4 with material quality ratio:1.
Gleditsia sinensis extract solution of the present invention through hot water also to raise, and its preparation method is：(can the gleditsia sinensis of remove seed
Be cut into section) clean up and be put into container, after adding water big fire boil small fire endure slowly to gleditsia sinensis it is soft it is rotten (water be gleditsia sinensis 5 times of left sides
It is right), after cooling, filtering.The stoste after filtering is refunded in pot again, after infusion 1 hour, cooled down, filtering will be mixed extract twice
It is described gleditsia sinensis extract solution to close.
It is a kind of to suppress phytopathogen, improve soil property and/or improve the method for continuous cropping obstacles, the present invention is provided
Microbial bacterial agent is applied to farmland, and the dosage used is 100L/ mus~1000L/ mus.
The mode of the administration is selected from：Root is filled, sprinkling, smear, infiltration, or microbial bacterial agent be adsorbed in filler entered
Row is broadcasted sowing.The filler is selected from diatomite, perlite, red beautiful soil or peat soil.
The invention provides a kind of microorganism formulation and preparation method and application, the microorganism formulation uses withered grass gemma
Bacillus fermentation is made, shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate and composite fertilizer in the nutrient solution of fermentation,
So that said preparation can not only play a part of suppressing harmful bacteria, additionally it is possible to provide substantial amounts of nutriment for crop.It is real
Test and show, microbial inoculum is made to the inhibition zones of pathogenic bacteria up to more than 6 times of control group in the microorganism formulation that provides of the present invention.Crop field
Experiment shows that the microbial inoculum has significant raising to the micronutrient levels in soil, improves the ginseng diseases caused by continuous cropping.
Embodiment
The invention provides a kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved, this area
Technical staff can use for reference present disclosure, be suitably modified technological parameter realization.In particular, all similar replacements
Apparent to those skilled in the art with changing, they are considered as being included in the present invention.The method of the present invention
And application is described by preferred embodiment, related personnel can substantially not depart from present invention, spirit and model
Enclose interior to methods herein and using being modified or suitably changing with combining, to realize and apply the technology of the present invention.
Examination material, the instrument that the present invention is used are all common commercially available product, can all be bought in market.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1
Bacillus subtilis 0.1kg, shrimp med 0.1kg, collagen 0.01kg, NaCl 9kg, amino acid/11 kg, carbon aquation
Compound 3kg, composite fertilizer 1kg.
Wherein, collagen is collagen, and amino acid is to add monohydrate zinc sulphate per in 80mL sea-tangle zymotic fluids
The hydrations of 20g and four ammonium heptamolybdate 1g is made, and carbohydrate is N/P/K=15/15/15 in sucrose, composite fertilizer.
Water 1000L, adds strain, shrimp shell meal, collagen, NaCl bubble aerations and is cultivated, cultivation temperature is maintained at
30 DEG C, incubation time is 3 days；
On the basis of once cultivating, amino acid, carbohydrate, composite fertilizer are added, continues bubble aeration and is cultivated,
Cultivation temperature is maintained at 30 DEG C, and incubation time is 4 days, produces microorganism formulation.
Embodiment 2
Bacillus subtilis 0.5kg, crab meat 0.1kg, collagen 1kg, NaCl 0.01kg, amino acid 5kg, carbon aquation
Compound 1kg, composite fertilizer 5kg.
Wherein, collagen is pigskin collagen, and amino acid is to add monohydrate zinc sulphate per in 120mL sea-tangle zymotic fluids
The hydrations of 40g and four ammonium heptamolybdate 3g is made, and carbohydrate is N/P/K=15/15/15 in fructose, composite fertilizer.
Water 1000L, adds strain, crab meat, collagen, NaCl bubble aerations and is cultivated, cultivation temperature is maintained at 28
DEG C, incubation time is 4 days；
On the basis of once cultivating, amino acid, carbohydrate, composite fertilizer are added, continues bubble aeration and is cultivated,
Cultivation temperature is maintained at 32 DEG C, and incubation time is 3 days, produces microorganism formulation.
Embodiment 3
Bacillus subtilis 0.5kg, bacillus thuringiensis 0.5kg, bacillus licheniformis 0.5kg, bacillus megaterium
0.5kg, crab meat 0.5kg, collagen 0.05kg, NaCl 0.9kg, amino acid 2kg, carbohydrate 3kg, composite fertilizer 3kg.
Wherein, collagen is collagen, and amino acid is to add monohydrate zinc sulphate per in 100mL sea-tangle zymotic fluids
The hydrations of 30g and four ammonium heptamolybdate 2g is made, and carbohydrate is N/P/K=15/15/15 in sucrose, composite fertilizer.
Water 1000L, adds strain, shrimp shell meal, collagen, NaCl bubble aerations and is cultivated, cultivation temperature is maintained at
30 DEG C, incubation time is 3 days；
On the basis of once cultivating, amino acid, carbohydrate, composite fertilizer are added, continues bubble aeration and is cultivated,
Cultivation temperature is maintained at 30 DEG C, and incubation time is 4 days, produces microorganism formulation.
Embodiment 4
Bacillus subtilis 0.3kg, bacillus thuringiensis 0.2kg, bacillus licheniformis 0.7kg, bacillus megaterium
0.3kg, shrimp crab powder 1kg, collagen 0.05kg, NaCl 0.05kg, amino acid 7kg, carbohydrate 10kg, composite fertilizer
5kg。
Wherein, collagen is collagen, and amino acid is to add sulfuric acid monohydrate in every 100mL sea-tangles zymotic fluid
The hydrations of zinc 40g and four ammonium heptamolybdate 1g is made, and carbohydrate is N/P/K=15/15/20 in glucose, composite fertilizer.
Water 1000L, adds strain, crab meat, collagen, NaCl bubble aerations and is cultivated, cultivation temperature is maintained at 32
DEG C, incubation time is 4 days；
On the basis of once cultivating, amino acid, carbohydrate, composite fertilizer are added, continues bubble aeration and is cultivated,
Cultivation temperature is maintained at 28 DEG C, and incubation time is 4 days, produces microorganism formulation.
Embodiment 5
Biological agent is made in each embodiment, after testing, inoculum density such as table 1：
Each embodiment inoculum density of table 1
| Inoculum density (cfu/ml) | |
| Embodiment 1 | 1.3×1013 |
| Embodiment 2 | 2.7×1011 |
| Embodiment 3 | 1.9×1012 |
| Embodiment 4 | 6.2×1011 |
As a result show, the microorganism formulation provided in various embodiments of the present invention has very high cell density.
Embodiment 6
Inhibition of the microbial-bacterial fertilizer to pathogen：
A. by microorganism formulation made from embodiment 1~4, being diluted respectively with water, adjustment cell density is about 1 ×
106cfu/ml, detects inhibitory action of each preparation to encountered pathogenic bacteria.
B. pathogen be Fusarium oxysporum, bought by CGMCC, with LB culture mediums (tryptone 10g, yeast extract 5g,
NaCl 10g, 1L water) 28 DEG C of cultures 1 day, it is diluted to 10 with sterilized water6Cfu/mL, it is standby..
C. culture medium is PDA culture medium, is made up of potato 200g, glucose 20g, agar 20g, 1L water, pH=6.5.
Observe inhibitory action of the bacterial manure to pathogen.
Test method：Odontothrips loti.
Sterilized PDA culture medium is heated to complete thawing, is poured in culture dish, per ware 15ml (lower floor), treats that it coagulates
Gu.With sterile working media surface vertically put Oxford cup (internal diameter 6mm, external diameter 8mm, high 10mm circular tubule, pipe
Two ends are smooth).In addition, the PDA culture medium of thawing is cooled into 50 DEG C or so is mixed into gained bacterium in 1mL b, bacterium will be mixed with
Culture medium 5ml is added on (upper strata) to be solidified on the culture medium solidified.Diluted each embodiment is added in Oxford cup to be made
Microorganism formulation (Oxford cup can typically fill 240 microlitres, not make its excessive).Fill it up with rearmounted 35 DEG C of overnight incubations, observation knot
Really.Every group parallel 3 times, average.If sterilized water is control.
Wherein, to the result of the test such as following table of embodiment 1~12：
The bacteriostatic experiment result of table 2
As a result show, control group is without inhibition zone, and all inhibition zones of experimental group are all higher than 18mm, illustrate that fungistatic effect is notable
(p<0.05) microorganism formulation that, each embodiment is provided all shows height bacteriostasis.Wherein, embodiment 3 and the institute of embodiment 4
The fungistatic effect for obtaining microorganism formulation is significantly better than embodiment 1 and embodiment 2, illustrates bacillus subtilis and the golden gemma bars of Su Yun
Bacterium, which coordinates, generates synergy synergy, so that (the p that is significantly improved to the inhibitory action of pathogen<0.05).
Embodiment 7~10
Formula such as table 3：
The microbial bacterial agent component of table 3
| Microorganism formulation | Water | Surfactant | |
| Embodiment 7 | The preparation 1L of embodiment 1 | 10L | Gleditsia sinensis extract solution 15g |
| Embodiment 8 | The preparation 1L of embodiment 2 | 1L | Glycine betaine 6mL |
| Embodiment 9 | The preparation 1L of embodiment 3 | 5L | Soapberry extract 10mL |
| Embodiment 10 | The preparation 1L of embodiment 4 | 5L | Soapberry extract 10mL |
Microorganism formulation is diluted after 1~10 times, is mixed with surfactant, and microbial bacterial agent is made.
Embodiment 11
It is the ginseng of precious Chinese medicine again as conventional Chinese medicine, can be not only used for medicine and can be used for health products and food again, absolutely
Most of obtained by cultivating.
Ginseng is perennial plant, and it is extremely strong to avoid ground property, it is impossible to continuous cropping continuous cropping, and it is general to continue planting ginseng with second crop soil
Seedling rate was deposited after the 2nd year and is down to less than 30%, and there is pest and disease damage mostly in the ginseng of survival, or even the plot having almost can be complete
The exhausted seedling in portion, current mechanism is not fully understood.Present ginseng crop rotation interval is usually more than 20 years, so in terms of cultivation
Continuous cropping obstacle is maximum technical barrier.
The soil of present invention selection ginseng continuous cropping 3 years, same plot randomly selects 8 pieces of 9m × 9m sample prescription, routinely turn over,
After applying fertilizer to the subsoil, root fills different given the test agent, then planting ginseng, and (according to required situation about keeping the skin wet) is every as needed afterwards
7~30 Tiangengs are filled or sprayed successively；Another set gives the processing of equivalent clear water as a control group.The respectively plantation 100 of experimental group and control group
Ginseng-leaf, it is in one line, it is spaced, under same humidity, temperature, illumination condition, observe 2 years, as a result such as table 4, table 5：
Table 4：Each group microelement contents of soil
Other embodiment is above to be given only the control group of equivalent water using rear microelement contents of soil, as a result with reality
Apply example 1 similar.
Table 5：The each group incidence of disease (ginseng root rot)
| The incidence of disease (%) | |
| The preparation of embodiment 1 | 28% |
| The preparation of embodiment 2 | 30% |
| The preparation of embodiment 3 | 20% |
| The preparation of embodiment 4 | 24% |
| The microbial inoculum of embodiment 7 | 18% |
| The microbial inoculum of embodiment 8 | 14% |
| The microbial inoculum of embodiment 9 | 17% |
| The microbial inoculum of embodiment 10 | 19% |
| Water | 43% |
Embodiment 12
Pseudo-ginseng medicinal plant is the distinctive rare traditional Chinese medicine of China, thin with strengthen immunity, antifatigue, protection cardiac muscle
The multiple efficacies such as born of the same parents, suppression Apoptosis, stomach invigorating, anti-inflammatory, the scattered stasis of blood, hemostasis, are widely used on human health care and treatment, have
High medicinal and economic value.At present, wild pseudo-ginseng is very rare, most pseudo-ginseng on medicine and food products market
From artificial cultivation.The artificial growth of pseudo-ginseng and pseudo-ginseng and cultivation are respectively positioned at the northeast of China and southwestern each province.In recent years,
Only the notoginseng planting area of the white county of Jilin governor and Wenshan Prefecture of Yunnan Province two places the period of full bloom more than 50 000 mu (35,000,
000m2), domestic market is not only supplied, also a large amount of outlets underproduce to meet the market demand.Because large area is single year after year
Plantation, pseudo-ginseng belongs to the perennial plant of perennial root in addition, and happiness warm moist, and it is extremely strong to avoid ground property, there is extremely serious continuous cropping
Problem on obstacle.Pseudo-ginseng is after one batch of plantation, and the soil after broad-leaf forest clear felling was difficult to recover in 30~50 years, and formation is commonly called as
" always " abandons ground.Pseudo-ginseng is planted always and deposits seedling rate below 25% after general 3 years, and more than 75% pseudo-ginseng seedling is all because of Roots of Panax Notoginseng
Rot and lose.And remaining Roots of Panax Notoginseng is covered with disease scar, perithelium reddens, and fibrous root comes off, and burning must be serious.
The present invention selection notoginseng soil of 3 years, same plot randomly selects 8 pieces of 9m × 9m sample prescription, routinely turn over,
After applying fertilizer to the subsoil, root fills different given the test agent, then plants pseudo-ginseng, and (according to required situation about keeping the skin wet) is every as needed afterwards
7~30 Tiangengs are filled or sprayed successively；Another set gives the processing of equivalent clear water as a control group.The respectively plantation 100 of experimental group and control group
Pseudo-ginseng seedling, it is in one line, it is spaced, under same humidity, temperature, illumination condition, observe 2 years, as a result such as table 6, table 7：
Table 6：Each group microelement contents of soil
Other embodiment is above to be given only the control group of equivalent water using rear microelement contents of soil, as a result with reality
Apply example 1 similar.
Table 7：The each group incidence of disease (notoginseng root rot)
As a result show, micro member in soil can all be improved by giving the microorganism formulation of the invention provided or microbial bacterial agent
The content of element, reduces the incidence of disease of crop.Relative to give embodiment 1~4 offer microorganism formulation, give embodiment 7~
Better (the p of 10 microbial inoculums provided<0.05), illustrate that raising of the addition of surfactant to effect serves important work
With.The microbial inoculum or the effect of preparation prepared with bacillus subtilis and bacillus thuringiensis, bacillus licheniformis co-fermentation
All it is significantly higher than (p<0.05) product made from exclusive use fermentation of bacillus subtilis, illustrates that 3 kinds of strains compoundings are served good
Good synergy synergy, so that the effect to soil improvement is improved and causes crop disease situation to obtain bright continuous cropping
Aobvious suppression.
Afterwards by differently testing throughout the country, the microbial-bacterial fertilizer pair of present invention compounding natural surfactant is drawn
There is obvious effect in terms of preventing and treating soil-borne disease of crop, improvement Soil Micro-environment, raising crop quality, increase yield, the present invention
Environmental protection noresidue, it is environmentally friendly.
It the above is only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
Say, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of microorganism formulation, it is characterised in that by strain, shrimp and crab shells, collagen, sodium chloride, compound amino acid, carbon water
Compound and composite fertilizer are made；
The strain includes bacillus subtilis；
The strain, shrimp and crab shells, collagen, sodium chloride, amino acid, carbohydrate, composite fertilizer mass ratio for (0.1~
1)：(0.1~10)：(0.01~1)：(0.01~9)：(1~50)：(1~20)：(1~10).
2. microorganism formulation according to claim 1, it is characterised in that the strain also include bacillus thuringiensis,
Bacillus licheniformis, bacillus megaterium；It is the bacillus subtilis and bacillus thuringiensis, bacillus licheniformis, huge
The quantity ratio of bacillus is (0.5~5)：(0.5~5)：(0.5~5)：(0.5~5).
3. microorganism formulation according to claim 1, it is characterised in that the compound amino acid is by sea-tangle zymotic fluid, one
Water zinc sulphate and four hydration ammonium heptamolybdates compositions, wherein addition monohydrate zinc sulphate 20g in per 80mL~120mL sea-tangle zymotic fluids~
The hydration ammonium heptamolybdates of 40g and four 1g~3g.
4. microorganism formulation according to claim 1, it is characterised in that the carbohydrate is selected from glucose, fructose
Or sucrose.
5. microorganism formulation according to claim 1, it is characterised in that N, P, K mass ratio are (10 in the composite fertilizer
~20)：(10~20)：(10~20).
6. the preparation method of the microorganism formulation described in any one of Claims 1 to 5, it is characterised in that including：
Step 1：Strain, shrimp and crab shells, collagen, sodium chloride are mixed with water, first is carried out and ferments, nutrient solution is made；
Step 2：Compound amino acid, carbohydrate, composite fertilizer mix with the nutrient solution, carry out second and ferment, micro- life is made
Thing preparation；
The temperature of first fermentation is 25 DEG C~35 DEG C；Time is 3 days~5 days；Condition is bubble aeration；
The temperature of second fermentation is 25 DEG C~35 DEG C；Time is 3 days~5 days；Condition is bubble aeration.
7. the microorganism formulation described in any one of Claims 1 to 5 is suppressing phytopathogen, is improving soil property, improvement crop company
Make the application in obstacle.
8. application according to claim 7, it is characterised in that the phytopathogen is Fusarium oxysporum, epidemic disease bacterium etc.；
The crop is ginseng, pseudo-ginseng or glutinous rehmannia.
9. a kind of microbial bacterial agent, it is characterised in that including volumes below part：
1 part of microorganism formulation described in any one of Claims 1 to 5；
0.001 part~0.02 part of surfactant；
1 part~10 parts of water.
10. a kind of suppress phytopathogen, improve soil property and/or improve the method for continuous cropping obstacles, it is characterised in that will weigh
Profit requires that the microbial bacterial agent described in 9 is applied to farmland；The applied dose is 100L/ mus~1000L/ mus.
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| CN109320368A (en) * | 2018-11-08 | 2019-02-12 | 北京泰克美高新技术有限公司 | The biological agent and preparation method thereof for preventing and treating liriomyza bryoniae |
| CN110833075A (en) * | 2019-10-11 | 2020-02-25 | 北京泰克美高新技术有限公司 | Compound preparation and application thereof in treating pine plague wood infected with pine wilt disease |
| CN111018612A (en) * | 2019-12-20 | 2020-04-17 | 德州市元和农业科技开发有限责任公司 | Full-water-soluble drip-irrigation microbial inoculant particles and preparation method thereof |
| CN111285742A (en) * | 2020-04-01 | 2020-06-16 | 黄河三角洲京博化工研究院有限公司 | Special biofertilizer for moderate and severe saline-alkali soil and preparation method thereof |
| CN116711741A (en) * | 2023-07-27 | 2023-09-08 | 内蒙古多肽科技有限责任公司 | Application of microbial preparation in astragalus membranaceus planting |
| CN118077719A (en) * | 2024-04-25 | 2024-05-28 | 赤峰市乾铭农业科技发展有限公司 | Application of microbial preparation in platycodon grandiflorum planting |
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| CN116711741A (en) * | 2023-07-27 | 2023-09-08 | 内蒙古多肽科技有限责任公司 | Application of microbial preparation in astragalus membranaceus planting |
| CN116711741B (en) * | 2023-07-27 | 2023-11-24 | 内蒙古多肽科技有限责任公司 | Application of microbial preparation in astragalus membranaceus planting |
| CN118077719A (en) * | 2024-04-25 | 2024-05-28 | 赤峰市乾铭农业科技发展有限公司 | Application of microbial preparation in platycodon grandiflorum planting |
| CN118077719B (en) * | 2024-04-25 | 2024-07-09 | 赤峰市乾铭农业科技发展有限公司 | Application of microbial preparation in platycodon grandiflorum planting |
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