CN104031863A - Bacillus subtilis for antagonizing Bacillus subtilis of Fusarium oxysporum f. sp. heterophylla - Google Patents
Bacillus subtilis for antagonizing Bacillus subtilis of Fusarium oxysporum f. sp. heterophylla Download PDFInfo
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- CN104031863A CN104031863A CN201410263152.0A CN201410263152A CN104031863A CN 104031863 A CN104031863 A CN 104031863A CN 201410263152 A CN201410263152 A CN 201410263152A CN 104031863 A CN104031863 A CN 104031863A
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- heterophylly faalsestarwort
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Abstract
The invention relates to a Bacillus subtilis for antagonizing Bacillus subtilis of Fusarium oxysporum f. sp. Heterophylla. The bacterial strain is Bacillus subtilis BS-C and is registered and preserved in Common microorganism Center of Chinese Microorganism Strain Preservation and Management Committee; the preservation number is CGMCCNo.9145. The separation of the antagonistic bacteria can provide a new biocontrol agent for biological prevention and control of pseudostellaria root rot. The Bacillus subtilis BS-C provided by the invention can be used for efficiently inhibit the Fusarium oxysporum f. sp. Heterophylla of pseudostellaria rhizosphere soil, and has wide application prospect in the field.
Description
Technical field
The present invention relates to a strain for the subtilis of antagonism Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum.
Background technology
Root of Heterophylly Faalsestarwort (
pseudstellariae heterophylla) be a kind of famousr and precious Chinese medicinal materials, containing amino acid, carbohydrate, glycoside, phospholipid, cyclic peptide and trace element etc.Have invigorating qi and benefiting blood, the effect of the tonifying the spleen and stomach that promotes the production of body fluid, be suitable for children's's summer heat of a specified duration and do not move back, cough, diet is depressed, the disease of weakness such as palpitaition and children's be weak unable, night sweat, mouth equality disease after being ill.Day by day deep along with Root of Heterophylly Faalsestarwort pharmacological effect research, Root of Heterophylly Faalsestarwort, as help medicine, becomes one of conventional bulk medicinal materials of China gradually.
Root of Heterophylly Faalsestarwort is of many uses, and the market requirement is very large.But its continuous cropping obstacle is extremely serious, the in the situation that of planting Root of Heterophylly Faalsestarwort continuously in same piece of land, even in the situation that enhancing field management, its upgrowth situation variation, even total crop failure, its product qualitative change is bad, Zong polysaccharide and content of ginsenoside significantly decline.Applicant is through studies confirm that: after Root of Heterophylly Faalsestarwort continuous cropping, Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum in rhizosphere soil (
fusarium oxysporumf. sp.
heterophylla) quantity extremely significantly increases, continuous cropping Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum (
fusarium oxysporumf. sp.
heterophylla) quantity be contrast nearly 5 times; The Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum being separated to is inoculated in the healthy soil of plantation Root of Heterophylly Faalsestarwort, Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum breeds in a large number in healthy soil, and infects Root of Heterophylly Faalsestarwort root, causes Root of Heterophylly Faalsestarwort disease.
In recent years, along with the research of the particular mechanism of action of green agriculture, Organic farming and microbial control is just flourish, obtaining efficient Antagonistic Fungi and making its effect perform to maximum was the basis of biological control.Many to the biological control of Fusarium oxysporum research in other crops (as banana, watermelon, cucumber, strawberry etc.), the Antagonistic Fungi kind of separated report is also more comprehensive.As mould in wood, bush mycorrhizal fungi (AM), Nonpathogenic Fusarium oxysporum, Paecilomyces lilacinus, false single-cell bacteria, genus bacillus, actinomycetes etc., such as the beautiful grade of old snow, once Bacillus subtilis BRF-2 was used for to cucumber and tomato wilt, but this is studied separated bacterial strain and compares source difference with them, for object also different, because Fusarium oxysporum exists the cause of genetic polymorphism.As a kind of universal soil-borne disease fungal pathogens, the pathogenic strains in Fusarium oxysporum kind has the host preference of height, from the Bacillus subtilis BRF-2 of elsewhere separation, might not have good effect to Root of Heterophylly Faalsestarwort specialized form sickle-like bacteria.The separated Antagonistic Fungi kind for Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum only has the < < of this seminar for the Paenibacillus polymyxa > > (patent No.: CN 102676435 B) of antagonism Root of Heterophylly Faalsestarwort rhizosphere soil Fusarium oxysporum at present, yet there are no the relevant report for the bacillus amyloliquefaciens of Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum.This seminar in early-stage Study, by dilution be coated with flat band method and dull and stereotyped dual test from experimental plot, Root of Heterophylly Faalsestarwort GAP base, Xi Yuan village, Ningde City Zherong County, Fujian Province, gather the subtilis that has been separated to a strain capable of high-efficiency continuous cropping Root of Heterophylly Faalsestarwort Rhizosphere sampling and suppressed Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum (
bacillus subtilis), called after BS-C.The biological control that the separation of this Antagonistic Fungi can be Root of Heterophylly Faalsestarwort root rot provides new biocontrol fungicide.
Summary of the invention
For this reason, the present invention screens a strain for the subtilis of antagonism Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum from Root of Heterophylly Faalsestarwort continuous cropping rhizosphere soil.
A strain provided by the invention is for the subtilis of antagonism Root of Heterophylly Faalsestarwort rhizosphere soil Root of Heterophylly Faalsestarwort specialized form sickle-like bacteria, for subtilis (
bacillus subtilis) BS-C, in the registration preservation of in May, 2014 12 China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number is CGMCCNo.9145.
This bacterium can be in genus bacillus collective media cultivation and fermentation.Medium component: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 5g/L, pH 7.0.
(eyepiece 10X, object lens 100X) lower this bacterium gramstaining result of observing under oily mirror, this bacterium is bacillus, gramstaining is negative.This bacterial strain is through morphology and 16s RNA molecular biology identification, be defined as subtilis (
bacillus subtilis).
This bacterial strain can more extensively utilize carbon source, comprise: L-rhamnosyl, sucrose, D-Glucose and PEARLITOL 25C, but part carbon source is not utilized, being comprised: D-Fructose, D-wood sugar, L-arabinose, L-inositol, maltose and cellobiose, also can more extensively utilize nitrogenous source.
Subtilis of the present invention (
bacillus subtilis) BS-C, can efficiently suppress the specialized form Fusarium oxysporum of Root of Heterophylly Faalsestarwort rhizosphere soil, having a extensive future of this field.This bacterial strain has been protected finish in the registration of in May, 2014 12 China Committee for Culture Collection of Microorganisms common micro-organisms center, it is called for short CGMCC, and address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is CGMCC N0.9145.
Accompanying drawing explanation
Fig. 1 be subtilis (
bacillus subtilis) BS-C is at NA substratum growth figure.
Fig. 2 be subtilis (
bacillus subtilis) fungistatic effect of BS-C to Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum.Left figure is the contrast of Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum; Right figure is the fungistatic effect of subtilis BS-C antagonism Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum.
Fig. 3 be subtilis (
bacillus subtilis) BS-C is to Root of Heterophylly Faalsestarwort toxicity test.In picture, first row is for not adding the potted plant seedling of contrast Root of Heterophylly Faalsestarwort of subtilis BS-C, and second row is the potted plant seedling of Root of Heterophylly Faalsestarwort adding after the processing of subtilis BS-C.
Fig. 4 be subtilis (
bacillus subtilis) BS-C, Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum Root of Heterophylly Faalsestarwort tissue cultured seedling train test altogether.
Embodiment
1, the isolation and purification of subtilis BS-C
Collection 2012 experimental plots, Root of Heterophylly Faalsestarwort GAP base, Xi Yuan village, Ningde City Zherong County, Nian Cong Fujian Province of soil sample gather continuous cropping Root of Heterophylly Faalsestarwort Rhizosphere sampling.Adopt 5 point sampling methods, mix as sample.
Adopt isolation by dilution method to the separation and purification of soil sample microorganism, take 10g soil and add in the triangular flask that 90mL sterilized water is housed, 120r/min, vibration 30min, gained soil supension is for the separation of related microorganism.The soil supension having shaken is made to 10
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6, 10
-7etc. the different dilution soil solution.The suspension of above-mentioned dilution is applied in and LB substratum is housed (fills a prescription as follows: Tryptones (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/L, sodium-chlor (NaCl) 10g/L, with NaOH, regulate the pH of this substratum, to 7.0 ~ 7.4, every liter adds 10g ~ 15g agar powder) flat band method on, each concentration repeats 3 times, be placed in the incubator overnight incubation of 30 ℃, choose the suitable flat board of colony number, picking single bacterium colony wherein, adopts the method for parallel scribing to carry out purifying, continuous 3 switchings, confirm as single bacterium.The soil microorganisms of getting respectively after separation and purification is inoculated on NA substratum, 30 ℃ activate 2 days, then receive on PDA substratum, with punch tool, beat and get Root of Heterophylly Faalsestarwort Fusarium oxysporum bacterium cake and receive PDA (potato 200g/L, glucose 20g/L, every liter, agar adds 15 ~ 20 grams, natural PH) carry out flat board face-off experiment on flat board, the bacterial strain of choosing obvious antagonistic action carries out preserving after purifying, standby.
2, the screening of subtilis BS-C and fungistatic effect are measured
Adopt face-off culture method further to screen each bacterial strain that has certain antagonistic action of above-mentioned purifying and Root of Heterophylly Faalsestarwort Fusarium oxysporum: the bacterium liquid 100 μ L that take out above-mentioned purifying pour on LB plate culture medium, with the glass painting rod of sterilizing, diluent is coated with uniformly, cultivates 7 days for 30 ℃.On good PDA flat board, with the plate culture medium that diameter 5mm punch tool is 9cm at diameter, punch on one side, access Antagonistic Fungi bacterium piece, and by the onesize Fusarium oxysporum bacterium piece access the other end, carry out flat board face-off and cultivate.Screening antagonistic effect good bacterial strain identifies it, through morphology and 16s rRNA molecular biology identification, be defined as subtilis (
bacillus subtilis), called after BS-C.
Subtilis BS-C bacterial strain ferments through seeding tank, further amplifies after fermentation to pilot scale fermentation tank, and after Root of Heterophylly Faalsestarwort plantation, spray application, in soil, or after the fermentation of bacterium liquid, is mixed base manure and is applied to the soil that is about to plant Root of Heterophylly Faalsestarwort immediately.Bacterium liquid spray application or mix base manure and use, its application concentration is 10
6-10
8cFUs/mL.After using, will significantly improve Root of Heterophylly Faalsestarwort yield and quality.
3, the fungistatic effect of subtilis BS-C is measured
In laboratory, with punch tool, Fusarium oxysporum and subtilis BS-C respectively being broken into diameter is the small bacteria block of 5mm the two ends that moved into respectively PDA plate culture medium.The bacterium piece of not inoculating the PDA plate culture medium central position access formed objects of this bacterium shines for empty.28 ℃ of constant temperature culture 5 days, carry out that colony diameter size is measured and inhibiting rate calculates, and determine the antagonism performance of bacterial strain.
Bacteriostasis rate calculation formula: Fusarium oxysporum colony diameter * 100% of bacteriostasis rate (%)=(the Fusarium oxysporum colony diameter of contrast-inoculate the Fusarium oxysporum colony diameter of this bacterium)/contrast.
As a result, this bacterial strain reaches 92.1% to 5 days bacteriostasis rates of Fusarium oxysporum, as shown in Figure 2.
4, subtilis BS-C bacterial strain toxicity test
By in this subtilis access 100mL LB substratum, each 3 bottles, cultivate after 3 days, this bacterium bacterium liquid 100 mL that get cultivation after diluting 5 times with sterilized water, apply Root of Heterophylly Faalsestarwort potted plant in, observe the growing state of Root of Heterophylly Faalsestarwort every day, observe this subtilis to the whether toxic effect of Root of Heterophylly Faalsestarwort.As shown in Figure 3, compared with the control, the potted plant seedling well-grown of Root of Heterophylly Faalsestarwort group, shows that this subtilis BS-C, to not murder by poisoning of Root of Heterophylly Faalsestarwort growth itself, can not make Root of Heterophylly Faalsestarwort cause a disease.
5, subtilis BS-C, Root of Heterophylly Faalsestarwort Fusarium oxysporum and Root of Heterophylly Faalsestarwort tissue cultured seedling are trained test altogether.
This Antagonistic Fungi bacterium liquid of 200 μ L is joined around Root of Heterophylly Faalsestarwort tissue cultured seedling rhizosphere, cultivate after 2 days in tissue culture bottle edge access sickle-like bacteria, observe and make mutually effect between pathogenic bacteria, Antagonistic Fungi, Root of Heterophylly Faalsestarwort tissue cultured seedling.
As shown in Figure 4, Root of Heterophylly Faalsestarwort tissue cultured seedling well-grown, the Root of Heterophylly Faalsestarwort tissue cultured seedling that only accesses Root of Heterophylly Faalsestarwort Fusarium oxysporum is entered that Root of Heterophylly Faalsestarwort Fusarium oxysporum is infected and withered and final dead gradually.And in being connected to the Root of Heterophylly Faalsestarwort tissue culture bottle of Root of Heterophylly Faalsestarwort Fusarium oxysporum and subtilis BS-C, subtilis BS-C can suppress Root of Heterophylly Faalsestarwort Fusarium oxysporum and grows and form inhibitory area to Root of Heterophylly Faalsestarwort tissue cultured seedling, cause Root of Heterophylly Faalsestarwort Fusarium oxysporum cannot cross inhibitory area and Root of Heterophylly Faalsestarwort is caused a disease, and crown prince's seedling upgrowth situation is the same with blank grows fine.
6, the application prospect of subtilis BS-C
Plant diseases more than 80% is by fungus-caused, controls fungal disease of crucial importance for improving crop yield.Fusarium oxysporum is the soil-borne disease fungi of a quasi-representative, in soil and organic matter, quantity, enlivening more, its scope of living away from home is very extensive, can cause the 100 various plants generation blights such as melon, Solanaceae, banana, cotton, pulse family and flowers, in agriculture production, cause serious crop failure, reduce quality, impact supply and export trade, even cause person poultry poisoning, cause serious financial loss.Applicant is through studies confirm that: after plantation Root of Heterophylly Faalsestarwort, in rhizosphere soil, Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum quantity extremely significantly increases; The Fusarium oxysporum being separated to is inoculated in the healthy soil of plantation Root of Heterophylly Faalsestarwort, Fusarium oxysporum breeds in a large number in healthy soil, and infects Root of Heterophylly Faalsestarwort root, causes Root of Heterophylly Faalsestarwort disease.Approximately more than 50,000 mu of the annual total cultivated area of Root of Heterophylly Faalsestarwort in the whole nation, by existing market price budget, the whole nation output value is more than 5,000,000,000 yuan of Renminbi approximately, but after continuous cropping, in Root of Heterophylly Faalsestarwort rhizosphere soil, the pathogenic bacterium such as specialized form Fusarium oxysporum and pathogenic bacteria are increased, make output sharply drop to below 1/3 of the first planting (contrast), produce serious financial loss, be one and be badly in need of the major issue solving.Subtilis BS-C can efficiently suppress Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum, having a extensive future of this field.
Claims (1)
- One strain for the subtilis of antagonism Root of Heterophylly Faalsestarwort specialized form Fusarium oxysporum ( bacillus subtilis) BS-C, in the registration preservation of in May, 2014 12 China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number is CGMCCNo.9145.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586303A (en) * | 2016-03-28 | 2016-05-18 | 福建农林大学 | Korean pseudomonas and application thereof |
| CN107287139A (en) * | 2017-08-03 | 2017-10-24 | 北京泰克美高新技术有限公司 | A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved |
| CN113604377A (en) * | 2021-06-23 | 2021-11-05 | 山东京青农业科技有限公司 | Bacillus subtilis microbial inoculum and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100078419A (en) * | 2008-12-30 | 2010-07-08 | 경기도농업기술원 | Novel bacillus subtilis and microorganism agent comprising the strains for preventing sclerotinia rot and fusarium wilt of plants |
| CN102676435A (en) * | 2012-05-24 | 2012-09-19 | 福建师范大学福清分校 | Paenibacillus polymyxa for antagonizing Fusarium oxysporum in rhizosphere soil of Radix Pseudostellariae |
-
2014
- 2014-06-14 CN CN201410263152.0A patent/CN104031863B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100078419A (en) * | 2008-12-30 | 2010-07-08 | 경기도농업기술원 | Novel bacillus subtilis and microorganism agent comprising the strains for preventing sclerotinia rot and fusarium wilt of plants |
| CN102676435A (en) * | 2012-05-24 | 2012-09-19 | 福建师范大学福清分校 | Paenibacillus polymyxa for antagonizing Fusarium oxysporum in rhizosphere soil of Radix Pseudostellariae |
Non-Patent Citations (3)
| Title |
|---|
| 太子参连作障碍及其根际土壤尖孢镰刀菌数量变化: "林茂兹等", 《云南农业大学学报》, 31 December 2012 (2012-12-31), pages 716 - 721 * |
| 孙晓宇等: "枯草芽孢杆菌BSK1对尖孢镰刀菌(Fusarium oxysporum)拮抗作用的研究", 《陕西农业科学》, 31 December 2012 (2012-12-31), pages 15 - 17 * |
| 柳慧丽: "拮抗枯草芽孢杆菌KC-5的分离鉴定及其发酵优化", 《中国生物工程杂志》, vol. 34, no. 3, 31 March 2014 (2014-03-31), pages 96 - 102 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586303A (en) * | 2016-03-28 | 2016-05-18 | 福建农林大学 | Korean pseudomonas and application thereof |
| CN105586303B (en) * | 2016-03-28 | 2019-03-12 | 福建农林大学 | One plant of South Korea pseudomonad and its application |
| CN107287139A (en) * | 2017-08-03 | 2017-10-24 | 北京泰克美高新技术有限公司 | A kind of microorganism formulation and preparation method thereof and the application in continuous cropping obstacle is improved |
| CN113604377A (en) * | 2021-06-23 | 2021-11-05 | 山东京青农业科技有限公司 | Bacillus subtilis microbial inoculum and preparation method and application thereof |
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