CN102994419B - Bacillus pumilus LNXM12 and application thereof - Google Patents
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Abstract
The invention relates to a bacillus pumilus LNXM12 China general microbiological culture collection center (CGMCC) No. 3380 and an application of the bacillus pumilus LNXM12 in preventing and treating damages of wheat scab, wherein the bacillus pumilus LNXM12 can grow easily at low temperature, has growth-promoting effect and disease prevention effect and is scattered in Tibet. The bacillus pumilus is biocontrol bacillus obtained by separating wheat rhizosphere soil in Nyemo County, Lhasa, Tibet; the bacillus pumilus can grow well under the low temperature condition, thereby being beneficial to the growth-promoting function and the biocontrol function; the biocontrol effect on the wheat scab can reach 68%, thereby increasing the yield of the wheat by 12%; and therefore, the bacillus pumilus has good application prospect.
Description
Technical field
The invention belongs to microorganism and agricultural disease Prevention Technique field, relate to bacillus pumilus, be specifically related to suitable raw bacillus pumilus (Bacillus pumilus) LNXM12 of a kind of low temperature and the application in control gibberella saubinetii disease thereof.
Background technology
Withered, the rotten wheat head of the wheat scab another name wheat head, red wheat head are the important diseases of Wheat and barley, all over the world, are mainly distributed in moist and semi-humid region, are especially injured seriously with the moistening rainy Temperate Region in China of weather.All can be injured to heading from seedling, mainly cause that seedling is withered, brown foot rot, stalk are rotten and fringe is rotten, wherein cause harm the heaviest with fringe corruption again.Wheat scab is on the south China Huaihe River for a long time always and one of the most serious disease occurs middle and lower reach of Yangtze River Mai Qu, also repeatedly occurred to be very popular at Huang-Huai-Hai plain Mai Qu, northwest Mai Qu and northeast spring wheat in recent years, the production loss caused in the time of being very popular can reach 10%-40%.Head blight not only causes the minimizing of wheat class output, also makes infected seed lose kind of use and industrial value, simultaneously because the low product of thanking of gibberella contains toxin, also can cause poisoning after people and animals are edible.
Prevent and treat the Main Means of wheat scab at present for spraying chemical pesticide, the extensive application of the latter not only causes the pollution of air, water source, soil and food, the prior orthoselection being through chemical pesticide makes many pathogenic bacterias create resistance, control for this disease brings larger difficulty, serious threat China wheat class production safety.In recent years, increasing area adopts the method for integrated control to prevent and treat wheat scab, wherein especially plays an important role with biological pesticide.Rhizospheric microorganism is of a great variety, wherein have many types can Promoting plant growth, controlling plant diseases, increase crop yield, this quasi-microorganism is commonly referred to as plant growth-promoting rhizobacteria (Plant Growth-Promoting Rhizobacteria is called for short PGPR).There is good mutualism relation between PGPR and plant, plant can not only be made to produce induced resistance, and the yield and quality of crop can be improved.So, be separated from PGPR and utilize the probiotic bacterium the control and the increase yield that are applied to Plant diseases with growth-promoting and prophylaxis effect, there is very important theory significance and using value.
CN 101836638A discloses a kind of sterilant preventing and treating wheat scab, and its effective constituent is triazolone and tebuconazole.This sterilant stable chemical nature, triazolone and tebuconazole two composition mutual tolerance during mixing, there is not chemical reaction, of many uses, synergy is remarkable, use safety, and there is good bacteriostatic activity in the gibberella saubinetii colony dissimilar for current field, and apparently higher than triazolone or tebuconazole single dose, namely both are composite synergism.This built agent also has good preventive effect for other important diseases such as wheat powdery mildew in Wheat Production, and fungicidal spectrum is wide, and can delay pathogenic bacteria and develop immunity to drugs to the single dose in formula, has great importance for resistance management.
CN 101617698A discloses the compound method that a kind of plant medical agent prevents and treats wheat scab, 5000 grams, pine needle, 2000 grams, tobacco leaf, nux vomica 3000 grams, withered 8000 grams of tea, 3000 grams, trypterygine powder, lime sulfur mixture 5000 grams.Get that pine needle, tobacco leaf, nux vomica, tea are withered, trypterygine powder pulverizes respectively to be grouped together and puts into large iron pan in proportion; add 200 jin of warm water soaking 5 minutes, then big fire infusion 30 minutes truce filtering extracting liquids and the abundant mix and blend of lime sulfur mixture 10 minutes finished product.
CN 102405908A discloses a kind of chitosan oligosaccharide composition for anti-wheat scab and uses thereof and method, particularly, relate to a kind of pesticide composition for anti-wheat scab, comprise oligochitosan in this pesticide composition, the weight percent that oligochitosan accounts for this pesticide composition is 0.003%-0.028%.This invention additionally provides the above-mentioned pesticide composition comprising oligochitosan for preventing and treating the purposes of wheat scab, and the weight percent that it is characterized in that comprising oligochitosan in the pesticide composition of oligochitosan is 0.003%-0.028%.This inventive composition solves the major issue of anti-wheat scab, especially with comprising the pesticide composition of oligochitosan after the seed and plant of process wheat, not only anti-wheat scab, and can not produce poisoning to crop, not having pesticide residue.
CN 1305721A discloses a kind of vegetable active oligosaccharide agricultural chemicals preventing and treating the crop pests such as wheat scab, described vegetable active oligosaccharide agricultural chemicals is take plant slag as raw material, obtain by following technique: in plant slag raw materials, add polygalacturonase with 10-15 ten thousand polygalacturonase unit/per kilogram raw material slag, be heated with stirring to 30-48 DEG C of hydrolysis, deactivation, solution after deactivation after filtration, screening after again through vacuum-concentrcted process, namely sterilizing potting obtains this product.
Have not yet to see the play-by-play utilizing PGPR to prevent and treat wheat scab.
Summary of the invention
One of the object of the invention is that provide a kind of bacillus pumilus (Bacillus pumilus), it has good fungistatic effect to pathogenic bacterias such as wheat scabs, has potential biological and ecological methods to prevent plant disease, pests, and erosion to be worth for Problems existing in current wheat scab control.Two of the object of the invention is to provide this bacillus pumilus preventing and treating the application in wheat scab.
For one of achieving the above object, the present invention adopts following technical scheme:
A kind of bacillus pumilus, Classification And Nomenclature is bacillus pumilus (Bacillus pumilus) LNXM12, and on November 2nd, 2009 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCC No.3380.
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 is separated to obtain from the wheat rhizosphere soil of Lhasa Nimu County.
The morphological specificity of described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 and physiological and biochemical property: Gram-positive, shaft-like, gemma can be produced, can 10 DEG C of growths, but can not 50 DEG C of growths, the cultivation of pH5.7 and 7%NaCl can be tolerated, catalase test, V-P test, Citrate trianion utilize test, glucose fermentation test and casein test to be the positive, and Starch Hydrolysis test, nitrate reduction test, experiment of growth factor and propionic salt utilize test to be feminine gender.
The Molecular Identification feature of described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380: the part 16S rRNA sequence utilizing round pcr amplification bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380, obtaining length is the fragment of 485bp, its sequencing result is as shown in sequence table, through carrying out tetraploid rice with known 16S rRNA sequence in GenBank, find that with the similarity of Bacillus pumilus GQ911554.1 be 99%.
According to the result of the gramstaining of bacterial strain, morphological specificity, physiological and biochemical property and 16S rRNA sequential analysis, with reference to " bacillus ", (Cai Miaoying etc. translate, agricultural press, 1988) and " microbial taxonomy " (Zhang Jizhong, Fudan University in Shanghai press, 1995) key is retrieved, and bacterial strain of the present invention is bacillus pumilus (Bacillus pumilus), called after LNXM12 through Bacillus subtilis.
Microbial inoculum containing bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 also belongs to protection scope of the present invention.
For achieve the above object two, the present invention adopts following technical scheme:
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 is preventing and treating the application in wheat scab.Concrete technical scheme is as follows:
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant fungistatic effect for gibberella.
Described bacillus pumilus (Bacillus pumilus) LNXM12C GMCC No.3380 has significant fungistatic effect for rice leaf spot bacteria.
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant fungistatic effect for yeast.
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant growth promoting effects effect for wheat.
Described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant growth promoting effects effect for Arabidopis thaliana.
The application method of described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 bacterial strain is: fermentation culture → dilution → sprinkling → effect measuring.
The fermentation culture of described bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 bacterial strain, its substratum consists of: glucose 20g/L, Pidolidone 5g/L, MgSO
40.5g/L, KCl0.5g/L, KH
2pO
41g/L, Fe
2sO
46H
2o 0.15mg/L, MnSO
4h
2o 5.0mg/L, CuSO
45H
2o0.16mg/L, all the other are filtered water, are adjusted to pH7.0.
Bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 is a kind of genus bacillus wheat scab being had to good prevention effect, and be the suitable raw bacterium of a kind of low temperature, can grow at the temperature of 10 DEG C, environmentally safe, can significantly suppress gibberella, rice leaf spot bacteria and yeast, significantly can also promote wheat and Arabidopis thaliana growth, there is potential biological and ecological methods to prevent plant disease, pests, and erosion and be worth.
Accompanying drawing explanation
Fig. 1 is the gemma photo of bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCCNo.3380 of embodiment 2.
Fig. 2 is that bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCCNo.3380 of embodiment 6 is to the bacteriostatic action photo of gibberella.
Fig. 3 is that bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCCNo.3380 of embodiment 7 is to the bacteriostatic action photo of rice leaf spot bacteria.
Fig. 4 is that bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCCNo.3380 of embodiment 8 is to saccharomycetic bacteriostatic action photo.
Technical scheme of the present invention is further illustrated by embodiment below in conjunction with accompanying drawing; but following example is only simple and easy example of the present invention; do not represent or limit the scope of the present invention, interest field of the present invention is as the criterion with claims.
Embodiment
For better the present invention being described, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
Embodiment 1:
Adopt five point samplings, in the wheat rhizosphere soil of Lhasa Nimu County, dark 5-25mm place takes soil sample to load in sampler bag, has marked sampling time, place, vegetation.Take soil sample 5g, be dissolved in 45ml physiological saline, on shaking table, concussion mixing 30min, leaves standstill and makes clarification.Aspirate supernatant is respectively with sterilized water dilution 10
-1, 10
-2, 10
-3, 10
-4, 10
-5five concentration gradients, 80 DEG C of water-bath 10min.Draw 200 μ l and be coated with LB flat board, 37 DEG C of incubated overnight.After flat board grows bacterium colony, picking list bacterium colony, after line purifying, numbering is preserved, and screening obtains bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380.
Embodiment 2:
With reference to " bacillus ", (Cai Miaoying etc. translate, agricultural press, 1988) and " microbial taxonomy " (Zhang Jizhong, Fudan University in Shanghai press, 1995) test method, the morphological specificity of bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 obtain embodiment 1 and physiological and biochemical property detect.
Result shows, this bacterium Gram-positive, shaft-like, gemma can be produced as shown in Figure 1,10 DEG C of growths, but 50 DEG C of growths, can not can tolerate the cultivation of pH5.7 and 7%NaCl, catalase test, V-P test, Citrate trianion utilize test, glucose fermentation test and casein test to be the positive, and Starch Hydrolysis test, nitrate reduction test, experiment of growth factor and propionic salt utilize test to be feminine gender.
Embodiment 3:
Bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 embodiment 1 obtained is inoculated in LB liquid medium, 180rpm, 37 DEG C of concussions cultivate 10h, collected by centrifugation thalline, add N,O-Diacetylmuramidase and SDS broken wall after suspension, adopt phenol-chloroform method to extract genomic dna.
Be template with genomic dna, use forward primer PRB1601(5 '-GGATCCTAATACATGCAAGTCGAGCGG-3 ') (SEQ ID NO:1) and reverse primer PRB1602(5 '-GGATCCACGTATTACCGCGGCTGCTGGC-3 ') (SEQ ID NO:2) carry out pcr amplification to 16S rRNA.PCR condition is: 95 DEG C, 4min; 94 DEG C, 1min; 50 DEG C, 1min; 72 DEG C, 2min; 34cycle; 72 DEG C, 10min, 4 DEG C of preservations.Reclaim after PCR primer and send order-checking, through confirming that 16S rRNA partial gene sequence length is 485bp, its result, as shown in sequence table, is 99% with Bacillu spumilus GQ911554.1 similarity in GenBank.
Embodiment 4:
According to table 1 formulated 1C substratum:
Table 1
| Moiety | Concentration | 1×1C | 5×1C |
| Peptone | 0.7 | 4.2g | 21g |
| Sodium-chlor | 0.5 | 3g | 15g |
| Soyflour | 0.3 | 1.8g | 9g |
| Final volume | 600ml | 600ml |
Prepare soil extract as follows:
Joined by 500g soil in 1L distilled water, 98 DEG C of heating 45min, filter paper filtering, in 4 DEG C of preservations after autoclaving.
According to table 2 formulated main medium:
Table 2
| Moiety | Final concentration | Volume (ml) |
| 5×1C | 1× | 50 |
| 20% glucose | 0.1% | 1.25 |
| Soil extract | 10% | 25 |
| Water | 173.75 |
Bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 embodiment 1 obtained is inoculated in above-mentioned main medium, 37 DEG C of overnight incubation, get the right-angled intersection point of 5 μ l bacterium liquid points to distance solid medium plate center 2.5cm, each bacterial strain 5 repetition, be placed on respectively in 4 DEG C, 10 DEG C, 14 DEG C, 18 DEG C, 30 DEG C incubators and cultivate, record strain growth situation every day, record 15 days.
Result shows, bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 of the present invention can grow in the environment of temperature≤10 DEG C.The bacterial strain that can manifest bacterium colony at the temperature of 10 DEG C in one week is designated as suitable raw bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCCNo.3380 of low temperature, the result of test further shows that it can grow under the condition of 4 DEG C.
Embodiment 5:
Bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 embodiment 1 obtained is inoculated into and is seeded on LB substratum, incubated overnight, then bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCC No.3380 of activation is transferred in Landy substratum and carries out shake flask fermentation, temperature is 30 DEG C, shaking speed is 200rpm, fermentation time is 38 hours, obtains the fermented liquid of bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380.
Wherein fermention medium consists of: glucose 20g/L, Pidolidone 5g/L, MgSO
40.5g/L, KCl0.5g/L, KH
2pO
41g/L, Fe
2sO
46H
2o 0.15mg/L, MnSO
4h
2o 5.0mg/L, CuSO
45H
2o0.16mg/L, all the other are filtered water, are adjusted to pH7.0.
Embodiment 6:
Adopt dull and stereotyped face-off method, good PDA is dull and stereotyped, the gibberella block of diameter 1cm is placed in dull and stereotyped central authorities, at its surrounding distance 2cm place, symmetry places four filter papers, filter paper is made a call to the fermented liquid of bacillus pumilus (Bacillus pumilus) the LNXM12CGMCC No.3380 of the embodiment 5 of 5 μ l, in triplicate, cultivate 3 days in 25 DEG C of illumination boxs, observe bacillus pumilus (Bacillus pumilus) LNXM12CGMCC No.3380 to the fungistatic effect of gibberella.
As shown in Figure 2, visible average diameter of inhibition zone is 10mm to result, and namely bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant bacteriostatic action to gibberella.
Embodiment 7:
Adopt dull and stereotyped face-off method, cultured rice leaf spot bacteria 5ml is added in the NA substratum of 150ml, the flat board of falling NA, each flat board puts the filter paper of four sterilizings, the fermented liquid drawing bacillus pumilus (Bacillus pumilus) the LNXM12CGMCC No.3380 of the embodiment 5 of 5 μ l is beaten on filter paper, cultivate 3 days for 28 DEG C, observe bacillus pumilus (Bacillus pumilus) LNXM12 CGMCC No.3380 to the fungistatic effect of rice leaf spot bacteria.
Result as shown in Figure 3, the average diameter of inhibition zone of the visible left side and below bacterium colony is 16.5mm, and namely bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant bacteriostatic action to rice leaf spot bacteria.
Embodiment 8:
Suitable for the low temperature of embodiment 4 raw bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 is inoculated in 1C substratum and cultivates 8h, then be transferred in the Landy substratum of 10ml with the ratio of 1:100, ferment about 40 hours, collected by centrifugation supernatant liquor, for subsequent use in 4 DEG C of storages.
Fungal bacterial strain yeast to be measured is inoculated into overnight incubation in appropriate media, after measuring OD value, bacterium liquid is diluted to 10
7cfu/ml, mixed culture medium is made containing bacterium dull and stereotyped.To be cooled completely after, right-angled intersection punching (apart from plate center 2.5cm), suitable raw bacillus pumilus (Bacillus pumilus) the LNXM12CGMCC No.3380 of inoculation low temperature is in Landy substratum fermented supernatant fluid.Cultivate 2d under placing room temperature, observe for saccharomycetic fungistatic effect.
As shown in Figure 4, visible average diameter of inhibition zone is 28mm to result, and namely bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 has significant bacteriostatic action to yeast.
Embodiment 9:
Wheat seed clear water soaks 2h, vernalization under 28 DEG C of wet conditions, be seeded in the Turnover Box that aseptic soil is housed, fill with root when treating that stem and leaf of Wheat grows to 5 true leaves, average every strain stem and leaf of Wheat waters the fermented liquid of bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCC No.3380 of 20ml embodiment 5, and (concentration is 10
8cfu/ml), contrast with clear water.Extract 10 young plants after two weeks, measure plant height, root length and plant fresh weight.
Result shows, the stem and leaf of Wheat through the process of bacterium liquid is obviously good than the growing way of control group.After measured, plant height increment rate reaches 61.5%, and the long increment rate of root reaches 3.4%, and fresh weight increment rate reaches 124.5%.Namely bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 can remarkable wheat growth-promoting.
Embodiment 10:
Arabidopis thaliana seed, after clorox (effective Cl content is 5%) surface sterilization, is seeded on MS culture medium flat plate (9cm), and hot-house culture one week is for subsequent use.
Suitable for the low temperature of embodiment 4 raw bacillus pumilus (Bacillus pumilus) LNXM12 CGMCCNo.3380 is inoculated into overnight incubation in LB liquid nutrient medium, after measuring OD value, is quantitatively 10 by bacterial concentration
5cfu/ml.
Arabidopis thaliana seedling is with after above-mentioned bacterium immersion root 5min, and plant to MS flat board (20 × 20cm), each dull and stereotyped 4 seedlings, each bacterial strain arranges 3 repetitions.Hot-house culture 21d, takes out the dull and stereotyped fresh weight weighing every Arabidopis thaliana.Negative control is fresh liquid substratum leaching root.
Result shows, is 0.04935g with the Arabidopis thaliana mean fresh of bacterium liquid process, negative control 0.039444g, and namely bacillus pumilus provided by the invention (Bacillus pumilus) LNXM12 CGMCC No.3380 can significantly promote that Arabidopis thaliana grows.
Embodiment 11:
The fermented liquid of bacillus pumilus (Bacillus pumilus) the LNXM12 CGMCC No.3380 of embodiment 5 is diluted 100 times, starts before first 15 days to execute fermented liquid to wheat spraying in heading, afterwards at interval of spray in 7 days 1 time, spray 3 times altogether.
Result shows, the fermented liquid of bacillus pumilus of the present invention (Bacillus pumilus) LNXM12 CGMCCNo.3380 has the effect of preventing and treating wheat scab significantly and improving wheat yield, and its biocontrol effect can reach 68%, increase yield 12%.
It should be noted that and understand, when not departing from the spirit and scope of the present invention required by accompanying claim, various amendment and improvement can be made to the present invention of foregoing detailed description.Therefore, the scope of claimed technical scheme is not by the restriction of given any specific exemplary teachings.
Applicant states, the present invention illustrates proportioning raw materials of the present invention and preparation process by above-described embodiment, but the present invention is not limited to said ratio and preparation process, does not namely mean that the present invention must rely on above-mentioned preparation process and could implement.Person of ordinary skill in the field should understand, any improvement in the present invention, to equivalence replacement and the interpolation of ancillary component, the concrete way choice etc. of raw material selected by the present invention, all drops within protection scope of the present invention and open scope.
Sequence table: (SEQ ID NO:3)
TGAGCGTAGTTGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTGCGAGCAGTTACTCTCGCACTTGTTCTTCCCTAACAACAGTAGCTTTACGATCCGAAGACCTTCATCACTCACGCGGCGTTGCTCCGTCAGGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCATTACCCCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGACAGCCGAAACCGTCTTTCATCCTTGAACCATGCGGTTCAAGGAACTATCCGGTATTAGCTCCGGTTTCCCGGAGTTATCCCAGTCTTACAGGCAGGTTACCCACGTGTTACTCACCCGTCCGCCGCTAACATCCGGGAGCAAGCTCCCTTCTGTCCGCTCGACTTGCATGTATTAGGATCCA
Claims (1)
1. bacillus pumilus (Bacillus pumilus) LNXM12CGMCC No.3380 is suppressing the application in yeast;
Described bacillus pumilus (Bacillus pumilus) LNXM12 on November 2nd, 2009 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number is CGMCCNo.3380, is to be separated to obtain from the wheat rhizosphere soil of Lhasa Nimu County; Its morphological specificity and physiological and biochemical property are: Gram-positive, shaft-like, gemma can be produced, can 10 DEG C of growths, but can not 50 DEG C of growths, can tolerate the cultivation of pH5.7 and 7%NaCl, catalase test, V-P test, Citrate trianion utilize test, glucose fermentation test and casein test to be the positive, and Starch Hydrolysis test, nitrate reduction test, experiment of growth factor and propionic salt utilize test to be feminine gender.
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| UY36332A (en) * | 2014-12-29 | 2017-04-28 | Fmc Corp | COMPOSITIONS OF BACILLUS PUMILUS RT1279 AND METHODS OF USE TO BENEFIT THE GROWTH OF PLANTS |
| CN107467075B (en) * | 2017-09-21 | 2020-11-24 | 怀化学院 | Application of bacillus pumilus as rice growth promoter |
| CN109439593A (en) * | 2018-12-12 | 2019-03-08 | 沈阳农业大学 | A kind of bacillus pumilus preventing and treating false smut |
| CN109699681B (en) * | 2019-01-28 | 2020-10-23 | 江苏省农业科学院 | Bacillus pumilus ZR3-3 for preventing and treating bacterial diseases and application thereof |
| CN110577911B (en) * | 2019-09-17 | 2021-03-26 | 山东农业大学 | Bacillus pumilus and application thereof |
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