CN104651260A - Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis - Google Patents
Brevibacillus brevis BBC-3 and application thereof as well as preparation method of microbial inoculum of brevibacillus brevis Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention relates to an antagonistic bacterium for preventing the fungal diseases of broccoli as well as a preparation method and the application of a microbial preparation of the antagonistic bacterium, and belongs to the field of biological prevention and treatment of crop diseases. The invention provides a strain of brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622, a production method of a microbial preparation containing the brevibacillus brevis, and also the application of the brevibacillus brevis BBC-3 or the microbial preparation in preventing and treating the fungal diseases of the broccoli. The brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622 is obtained by virtue of preliminary screening and secondary screening, wherein a plate opposing test is performed for the preliminary screening and a liquid fermentation liquid is adopted for the secondary screening; the brevibacillus brevis BBC-3 is capable of effectively preventing and treating a plurality of fungal diseases of the broccoli, such as gray mold, downy mildew, stalk break, black rot and black spot, and has the prevention and treatment efficiency of above 70%; in addition, the brevibacillus brevis BBC-3 is good in environmental safety and has excellent development application prospect.
Description
Technical field
The present invention relates to a kind of Brevibacillus brevis BBC-3 for preventing and treating Caulis et Folium Brassicae capitatae fungal disease (
brevibacillus brevis) and microbial inoculum preparation and application, belong to field of biological control on crop disease.
Background technology
Caulis et Folium Brassicae capitatae is Cruciferae, one, biennial herb plant, belong to cabbage vegetables.The inanimate matter such as its rich vitamin A, vitamins C and phosphorus, iron, calcium, and the content of protein is also very high is the important foreign exchange earning vegetables of China.Fungal disease is the important disease of a class on brassicaceous vegetable, occurs general and endangers serious.
The current control to Caulis et Folium Brassicae capitatae fungal disease is mainly through planting anti-(resistance to) sick kind and chemical prevention controls.Along with harmful pathogenic bacteria resistance problem is day by day serious, and chemical pesticide is residual easily causes the serious harm such as residual hazard, environmental pollution, causes very large threat, be unfavorable for the Sustainable development of Caulis et Folium Brassicae capitatae cultivation and production to HUMAN HEALTH, ecotope.
Utilizing antagonistic microbe and meta-bolites thereof suppress or kill pathogen, is one of effective measure of control of plant disease.The harm that biological control not only can avoid chemical pesticide to bring, and safe and effective, persistent.Among numerous biocontrol microorganisms, genus bacillus growth is vigorous, and breeding rapidly, can produce resistance statospore, have other microorganisms cannot be equal to than advantage.
Research at present in the biological control of Caulis et Folium Brassicae capitatae fungal disease is also less, and especially the patent documentation of this respect temporarily has no report.The object of the present invention is to provide a kind of can the sporeformer of efficient antagonism Caulis et Folium Brassicae capitatae pathogenic fungi, and form microbial preparation, for development of new Caulis et Folium Brassicae capitatae fungal disease biological and ecological methods to prevent plant disease, pests, and erosion product lays the foundation.
Summary of the invention
The object of the present invention is to provide a kind of can the efficiently Brevibacillus brevis of the multiple pathogenic fungi of antagonism Caulis et Folium Brassicae capitatae and microbial preparation thereof, and provide the preparation method of this microbial preparation.Said preparation can effectively prevent and treat Caulis et Folium Brassicae capitatae fungal disease after using, and reduces the use of chemical pesticide, reduces environmental pollution, improves product quality.
The technical solution adopted in the present invention is as follows:
Brevibacillus brevis BBC-3 of the present invention has carried out preservation on August 28th, 2014 at China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center, and preserving number is: CGMCC No.9622.
The application of Brevibacillus brevis BBC-3 of the present invention in fungal diseases of plants control.
Further, fungal disease of the present invention comprises ash arrhizus bacteria, Pseudoperonospora cubensis, black rot, hyphal cluster germ, alternaria, phytophthora, anthrax bacteria, wilt, ash arrhizus bacteria or dry thread Pyrenomycetes.
Further, plant of the present invention is Caulis et Folium Brassicae capitatae.
Further, the PB of Brevibacillus brevis BBC-3 liquid described in the preparation method of the microbial inoculum of Brevibacillus brevis BBC-3 of the present invention substratum, 28 DEG C of shaking culture obtain fermented liquid, described Brevibacillus brevis BBC-3 bacterial content>=2.0 × 10
9cfu/mL, adopts spray-drying process to obtain solid powder.
Further, spray-dired matrix of the present invention is micro mist calcium carbonate, beta-cyclodextrin.
Further, fermented liquid of the present invention is 10 weight parts, and described micro mist calcium carbonate is 0.2 ~ 0.8 weight part, and described beta-cyclodextrin is 0.7 ~ 1.3 weight part.
Further, solid powder of the present invention is containing described Brevibacillus brevis BBC-3 sum>=2.5 × 10
10cfu/g.
The invention has the beneficial effects as follows provide a kind of effectively can prevent and treat Caulis et Folium Brassicae capitatae fungal disease antagonistic strain and microbial inoculum and application, this product tool has the following advantages: the Brevibacillus brevis that 1) the present invention announces has broad spectrum antibacterial, not only there is stronger antagonistic action to the multiple pathogenic bacteria of Caulis et Folium Brassicae capitatae, and to other pathogenic bacterias as phytophthora blight of pepper, cucumber anthracnose, cotton-wilt fusarium, botrytis cinerea pers, Botrytis cinerea, dry thread Pyrenomycetes, watermelon Pseudoperonospora cubensis etc. also have good antagonistic action.2) there is good growth-promoting functions: after microbiobacterial agent prepared by the present invention is used, effectively can not only suppress the generation of Caulis et Folium Brassicae capitatae fungal disease, but also nourishing and growing of Caulis et Folium Brassicae capitatae can be promoted, increase yield, reduce product pesticide residue.3) relative to chemical pesticide, microbiobacterial agent prepared by the present invention is safer, environmental protection, its preparation and use procedure pollution-free, nuisanceless, environmentally friendly.In a word, the Brevibacillus brevis microbial inoculum that the present invention announces uses in Caulis et Folium Brassicae capitatae cultivation and production has advantage that is nontoxic, pollution-free, noresidue, it is the effective way solving Caulis et Folium Brassicae capitatae pesticide residue and germ resistance problems, and this microbial inoculum also has certain application potential on other crops, significant to promotion Sustainable Development of Vegetable Industry.
biological sample preservation information
Brevibacillus brevis BBC-3, Classification And Nomenclature is
brevibacillus brevis, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center on August 28th, 2014, and preserving number is: CGMCC No. 9622; Preservation address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica, postcode 100101.
Accompanying drawing explanation
Fig. 1 is the phylogenetic tree of Brevibacillus brevis BBC-3 of the present invention.
Embodiment
The present invention is by a large amount of screening operation, obtain a strain has stronger antagonistic action bacterial strain BBC-3 to Caulis et Folium Brassicae capitatae fungal disease germ, by thalli morphology, physiological and biochemical property and 16S rDNA molecular biology identification, determine this bacterial strain be Brevibacillus brevis (
brevibacillus brevis).This bacterial strain is stored in China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center on September 5th, 2014, and preserving number is: CGMCC No. 9622; Preservation address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica, postcode 100101;
Single bacterium colony that Brevibacillus brevis bacterial strain BBC-3 of the present invention is formed at PB culture medium flat plate is circular, and opaque, smooth surface is moistening, neat in edge, and median rise does not produce soluble pigment.Thalli morphology is bacillus, produces gemma, raw in gemma, ovalize, Gram-positive, and thalline size is (0.6-0.8) μm × (3.5-4.0) μm.
Brevibacillus brevis bacterial strain BBC-3 of the present invention has stronger antagonistic action to pathogenic fungies such as Caulis et Folium Brassicae capitatae gray mold, oidium, Black Rotten, sclerotium disease, black spots.
Brevibacillus brevis bacterial strain BBC-3 of the present invention also has antagonistic action to vegetalitas pathogenic fungies such as phytophthora blight of pepper, cucumber anthracnose, cotton-wilt fusarium, botrytis cinerea pers, Botrytis cinerea, dry thread Pyrenomycetes, watermelon Pseudoperonospora cubensis.
Microbial preparation of the present invention contains the Brevibacillus brevis bacterial strain BBC-3 that preserving number is CGMCC No. 9622.Its liquid bacterial agent total plate count>=2.0 × 10
9cfu/mL, solid powder is containing total plate count>=2.5 × 10
10cfu/g.
The preparation method of microbial preparation of the present invention comprises the following steps:
Step one: the Brevibacillus brevis BBC-3 of preservation line is transferred in PB culture medium flat plate, is placed in 28 DEG C of constant temperature culture 2 d.The Brevibacillus brevis BBC-3 lawn of acquisition is inoculated in PB seed culture medium, 28 DEG C of shaking table shaking culture 24 h, obtained seed liquor.
Step 2: by the seed liquor of step one by volume 10% inoculum size be inoculated in bulk fermentation substratum, at 28 DEG C, cultivate 36 h, obtain fermented liquid.
Step 3: after the fermentation liquor microscopy of step 2 is counted, bacterial concentration>=2.0 × 10
9cfu/mL, aseptic subpackaged, the Brevibacillus brevis microbial liquid preparation obtained.
Step 4: liquid fermentation liquid step 2 obtained mixes by a certain percentage with multiple matrix, obtains solid powder further by spraying dry.
The bulk fermentation substratum described in preparation method's step 2 of microbial preparation of the present invention comprises extractum carnis 5.0 g/L, peptone 10.0 g/L, NaCl 5.0 g/L, glucose 20.0 g/L, pH 7.0 ~ 7.2; The matrix described in preparation method's step 4 of microbial preparation of the present invention is micro mist calcium carbonate, beta-cyclodextrin; Wherein fermented liquid: micro mist calcium carbonate: beta-cyclodextrin=10:(0.2-0.8): (0.7-1.3).
Microbial preparation of the present invention is applied to the control of Caulis et Folium Brassicae capitatae fungal disease, and effectively can suppress the growth of the pathogenic fungies such as Caulis et Folium Brassicae capitatae gray mold, oidium, Black Rotten, black spot, sclerotium disease, it is prevented and treated efficiency and reaches more than 70%.
Below in conjunction with preferred embodiment, technical scheme of the present invention is set forth in detail.Following examples only for instruction and explanation of the present invention, and do not form the restriction to technical solution of the present invention.
the separation of embodiment 1 Brevibacillus brevis BBC-3, screening and preservation
(1) soil sampling: select the Caulis et Folium Brassicae capitatae field of growing fine, at the rhizosphere of healthy Caulis et Folium Brassicae capitatae plant, scalp topsoil 2-3 cm, get soil 10 g closed on Caulis et Folium Brassicae capitatae rhizosphere; Load in sterilized kraft bag, seal sack, and record sampling spot, environment and date.
(2) strains separation and purifying: with plate dilution method, pedotheque is separated on PB substratum.Concrete operations are as follows: accurately take 10 g pedotheques, join in the triangular flask filling 90 mL sterilized waters and granulated glass sphere, and 200 rpm vibrate 30 min, and soil sample is evenly distributed becomes soil supension, and concentration is 10
-1; The rest may be inferred progressively 10 times of gradient dilutions to 10
-2, 10
-3, 10
-410
-7; Select 10
-5, 10
-6, 10
-7three concentration, at 80 DEG C of water bath with thermostatic control 20 min, are then respectively got 100 μ L diluent spread plates, are positioned over 30 DEG C of constant temperature culture carton upside downs and cultivate 48 h; Single bacterium colony of picking different shape, identifies with spore staining, is forwarded to PB substratum test tube slant after each bacterial strain method of scoring purifying 2-3 time, and cultivate 48 h for 30 DEG C, 4 DEG C of Refrigerator stores are for subsequent use.
(3) active bacterial strain screening:
Primary dcreening operation: with Caulis et Folium Brassicae capitatae pathogenic fungi for indicator, employing tradition five selects the restraining effect that face-off method mensuration different strains grows Caulis et Folium Brassicae capitatae pathogenic fungi.Concrete operation method is as follows: at the pathogenic fungi bacterium cake of PDA plate center inoculation diameter 5 mm, the equidistant inoculation of surrounding bacterial strain to be screened, 25 DEG C of constant temperature culture, each process 3 repetition.Only to inoculate Caulis et Folium Brassicae capitatae pathogenic fungi bacterium block for contrast.According to the antibacterial bandwidth determination antagonism degree between each screening bacterium colony edge and pathogenic bacteria colony edge, the bacterial strain that retention is excellent.
Multiple sieve: Antagonistic Fungi is inoculated in PB liquid nutrient medium (50 mL/250 mL), at 200 rpm, cultivates 48 h under 30 DEG C of conditions; By centrifugal for fermented liquid (8000 rpm, 20 min), abandon precipitation, collect supernatant; By supernatant liquor 0.22 μm of membrane filtration removing thalline, make aseptic ferment filtrate.With the punching of sterilizing punch tool on the pathogenic fungi flat board prepared, dull and stereotyped punching injects 100 μ L Antagonistic Fungi bacteria-free filtrates in backward hole, and pathogenic fungi screen plate 25 DEG C cultivates 3 ~ 5 d, adopts right-angled intersection method measure antibacterial circle diameter and calculate average inhibition.Filter out and can produce the metabolic activity product bacterial strain the strongest to Caulis et Folium Brassicae capitatae pathogenic fungi antagonistic action, called after BBC-3, has carried out-80 DEG C of freezings to by this bacterial strain.
the mensuration of embodiment 2 Brevibacillus brevis BBC-3 antimicrobial spectrum
Choosing the representative 15 kind of plant pathogenic fungies such as Caulis et Folium Brassicae capitatae gray mold, black spot, sclerotium disease, Black Rotten, phytophthora blight of pepper, cucumber anthracnose, cotton-wilt fusarium, ash arrhizus bacteria, Botrytis cinerea, dry thread Pyrenomycetes, fusarium graminearum, southern corn leaf blight, tomato early blight bacterium, Fulvia fulva Ciferri, Pseudoperonospora cubensis is target, adopts Agar diffusion test to measure the antimicrobial spectrum of bacterial strain BBC-3.Bacterial strain BBC-3 all has stronger antagonistic action to 16 kind of plant pathogenic fungies, and wherein the strongest to the restraining effect of Caulis et Folium Brassicae capitatae ash arrhizus bacteria, antibacterial circle diameter can reach 30.23 mm, and be secondly black rot, inhibition zone reaches 28.79 mm.
Table 1 Brevibacillus brevis BBC-3 is to the restraining effect of different plant pathogenic fungi
the qualification of embodiment 3 Brevibacillus brevis BBC-3
According to the experiment content recorded in " common bacteria system identification handbook " and test method, bacterial strain BBC-3 is obtained to screening and carries out morphology and Physiology and biochemistry qualification.Single bacterium colony that this bacterial strain is formed at PB culture medium flat plate is circular, and opaque, smooth surface is moistening, neat in edge, and median rise does not produce soluble pigment.Picking is cultivated after 24 h bacterium colonies carry out gramstaining, and in opticmicroscope 100 times of oily Microscopic observations, bacterial strain is bacillus, thalline size is (0.6-0.8) μm × (3.5-4.0) μm, produces gemma, Gram-positive, raw in gemma, ovalize.Below its Physiology and biochemistry identification of indicator:
Table 2 Brevibacillus brevis BBC-3 Physiology and biochemistry identification of indicator
Note :+represent positive reaction (exist or respond);-represent negative reaction (do not exist or reactionless); V represents reaction variable (reaction unstable between bacterial strain).
Identify after above-mentioned each index is compared in conjunction with 16S rDNA sequence this bacterial strain be Brevibacillus brevis (
brevibacillus brevis), its 16S rDNA sequence is shown in sequence table seq1.Send China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center to carry out preservation, preserving number is: CGMCC No.9622.
the preparation of embodiment 4 microbial preparation
The Brevibacillus brevis BBC-3 of preservation line is transferred in PB culture medium flat plate, as 28 DEG C of constant temperature culture 2 d; The Brevibacillus brevis BBC-3 lawn of acquisition is inoculated in PB seed culture medium, 28 DEG C of shaking table shaking culture 24 h, obtained seed liquor; By seed liquor by volume 10% inoculum size be inoculated in bulk fermentation substratum, at 28 DEG C, cultivate 36 h, obtain fermented liquid bacterium number>=2.0 × 10
9cfu/mL; By liquid fermentation liquid and opaque calcium carbonate and beta-cyclodextrin in 10:(0.2-0.8): the ratio of (0.7-1.3) mixes, and obtains solid powder further by spraying dry, containing total plate count>=2.68 × 10
10cfu/g.
Prepared by table 3 Brevibacillus brevis solid fungicide
the Field information test of embodiment 8 ~ 12 Brevibacillus brevis BBC-3 microbial preparation
The Field information test of microbial preparation of the present invention is carried out Zhangjiakou City Guyuan County, Hebei province Caulis et Folium Brassicae capitatae Tanaka.Become by mentioned microorganism preparation diluent 250 times, 500 times, 750 times, 1000 times, 1500 times, 2000 times to carry out Caulis et Folium Brassicae capitatae foliage-spray, with spray clear water for blank, 3 repetitions are established in each process.Start to spray in the Caulis et Folium Brassicae capitatae curd development phase, spray 3 times continuously, 15 days, interval, after last spray medicine, in 10 days, add up the incidence of Caulis et Folium Brassicae capitatae fungal disease.
The different spraying concentration of table 4 microbial preparation is to the prevention effect of Caulis et Folium Brassicae capitatae fungal disease
As can be seen from the above results, considering cost and effect, 750-1000 is doubly the optimum diluting multiple of this microbial preparation at Caulis et Folium Brassicae capitatae foliage-spray.Can 80.1% be reached to the prevention effect of oidium when diluting 750 times, effectively reduce sickness rate, promote to nourish and grow, volume increase 8.9%; When dilution 1000 times, 78.8% is reached to the prevention effect of gray mold, volume increase 7.0%; In addition said preparation at 500-1000 doubly to the protection effect of Caulis et Folium Brassicae capitatae black spot, Black Rotten, sclerotium disease all more than 70%.
To sum up, the preserving number that the present invention filters out is that the Brevibacillus brevis BBC-3 of CGMCC No.9622 has good antagonistic action to the multiple pathogenic fungi of Caulis et Folium Brassicae capitatae, its microbial preparation effectively can prevent and treat the generation of the multiple fungal disease of Caulis et Folium Brassicae capitatae, thus reach increase yield, reduce the object of product pesticide residue.
Claims (8)
1. a Brevibacillus brevis BBC-3, this Brevibacillus brevis has carried out preservation on August 28th, 2014 at China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center, and preserving number is: CGMCC No.9622.
2. the application of a kind of Brevibacillus brevis BBC-3 according to claim 1 in fungal diseases of plants control.
3. the application of a kind of Brevibacillus brevis BBC-3 according to claim 2 in fungal diseases of plants control, is characterized in that described fungal disease comprises ash arrhizus bacteria, Pseudoperonospora cubensis, black rot, hyphal cluster germ, alternaria, phytophthora, anthrax bacteria, wilt, ash arrhizus bacteria or dry thread Pyrenomycetes.
4. the application of a kind of Brevibacillus brevis BBC-3 according to claim 3 in fungal diseases of plants control, is characterized in that described plant is Caulis et Folium Brassicae capitatae.
5. the preparation method containing, for example the microbial inoculum of Brevibacillus brevis BBC-3 according to claim 1, it is characterized in that described Brevibacillus brevis BBC-3 liquid PB substratum, 28 DEG C of shaking culture obtain fermented liquid, bacterial content>=2.0 × 10 of described Brevibacillus brevis BBC-3
9cfu/mL, adopts spray-drying process to obtain solid powder.
6. the preparation method of the microbial inoculum containing Brevibacillus brevis BBC-3 according to claim 5, is characterized in that described spray-dired matrix is micro mist calcium carbonate, beta-cyclodextrin.
7. the preparation method of the microbial inoculum containing Brevibacillus brevis BBC-3 according to claim 6, it is characterized in that described fermented liquid is 10 weight parts, described micro mist calcium carbonate is 0.2 ~ 0.8 weight part, and described beta-cyclodextrin is 0.7 ~ 1.3 weight part.
8. the preparation method of the microbial inoculum containing Brevibacillus brevis BBC-3 according to claim 6-7 any one, is characterized in that described solid powder is containing described Brevibacillus brevis BBC-3 sum>=2.5 × 10
10cfu/g.
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| CN105998141A (en) * | 2016-05-10 | 2016-10-12 | 王秋红 | Pure-fermenting method for preparing mirabilitum praeparatum and application thereof |
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| CN107164438A (en) * | 2017-07-13 | 2017-09-15 | 陕西科技大学 | A kind of fermentation process for improving the Brevibacillus brevis bacteriocin level of production |
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| CN105998141A (en) * | 2016-05-10 | 2016-10-12 | 王秋红 | Pure-fermenting method for preparing mirabilitum praeparatum and application thereof |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| CN107164438A (en) * | 2017-07-13 | 2017-09-15 | 陕西科技大学 | A kind of fermentation process for improving the Brevibacillus brevis bacteriocin level of production |
| CN107164438B (en) * | 2017-07-13 | 2021-03-23 | 陕西科技大学 | Fermentation method for improving production level of brevibacillus brevis bacteriocin |
| CN107652026B (en) * | 2017-09-12 | 2020-07-17 | 湖北省农业科学院植保土肥研究所 | Special disease-resistant fertilizer for fingered citron and Chinese yam and preparation method thereof |
| CN109169650A (en) * | 2018-08-15 | 2019-01-11 | 山东泰诺药业有限公司 | A kind of preparation method of waxy Bacillus wettable powder |
| CN112980748A (en) * | 2021-05-07 | 2021-06-18 | 河北萌帮生物科技有限公司 | Brevibacillus brevis, application and method for producing humic acid by converting lignite |
| CN112980748B (en) * | 2021-05-07 | 2022-04-12 | 河北萌帮生物科技有限公司 | Brevibacillus brevis, application and method for producing humic acid by converting lignite |
| CN113265360A (en) * | 2021-06-10 | 2021-08-17 | 烟台市森林资源监测保护服务中心(烟台市林业技术推广中心、烟台沿海防护林省级自然保护区管理中心) | Brevibacillus brevis and application thereof |
| CN113265360B (en) * | 2021-06-10 | 2022-04-26 | 烟台市森林资源监测保护服务中心(烟台市林业技术推广中心、烟台沿海防护林省级自然保护区管理中心) | Brevibacillus brevis and application thereof |
| CN115029261A (en) * | 2022-05-17 | 2022-09-09 | 福建省农业科学院农业生物资源研究所 | Biocontrol compound microbial agent as well as preparation method and application thereof |
| CN115029261B (en) * | 2022-05-17 | 2023-05-16 | 福建省农业科学院农业生物资源研究所 | Biocontrol compound microbial agent and preparation method and application thereof |
| CN115281216A (en) * | 2022-08-10 | 2022-11-04 | 河北省科学院生物研究所 | Application of brevibacillus brevis ZLP-151 in biological prevention and control |
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