CN102461563A - Polyvalent protein attractant suitable for bactroceradorsalis (Hendel), its preparation method and application - Google Patents
Polyvalent protein attractant suitable for bactroceradorsalis (Hendel), its preparation method and application Download PDFInfo
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- CN102461563A CN102461563A CN2010105368581A CN201010536858A CN102461563A CN 102461563 A CN102461563 A CN 102461563A CN 2010105368581 A CN2010105368581 A CN 2010105368581A CN 201010536858 A CN201010536858 A CN 201010536858A CN 102461563 A CN102461563 A CN 102461563A
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Abstract
Belonging to the technical field of citrus pest control, the invention specifically relates to a polyvalent protein attractant suitable for bactroceradorsalis (Hendel), its preparation method and application. The polyvalent protein attractant comprises by weight: 5-20% of polyvalent peptone; 5-20% of brown sugar; 10-20% of edible vinegar or 1-3% of ammonium acetate; 0.1-0.3% of a preservative; 0-2% of methyleugenol; and 0.1-1% of a pesticide. Water is added to the above components to a full formulation amount, and through fully stirring, dissolving and uniformly mixing, a polyvalent protein attractant for effective control of bactroceradorsalis (Hendel) can be obtained. The polyvalent protein attractant of the invention has the advantages of substantial attraction effect for female and male imagoes of bactroceradorsalis (Hendel), no environmental pollution, no toxicity to human and other beneficial insects, as well as simple production technology and low cost, etc.
Description
Technical field
The invention belongs to the mandarin orange pest prevention and control field, be specifically related to a kind of citrus fruit fly multivalence protein attractant and preparation method thereof that is applicable to.
Background technology
Citrus fruit fly claim again dacus dorsalis hendel of mandarin orange Bactroceradorsalis (Hendel) in EMUs for Kyushu of Japan, the Ryukyu Islands and China Taiwan the earliest by report.Be diffused into the many countries and regions in North America, Oceania and Asia at present, between north and south latitude 20-30 degree, winter climate is caused harm the heaviest in the area more than 20 ℃.In China domestic citrus producing region, its mainly cause harm 46 sections more than 250 kind of fruit tree, vegetables and flowers such as oranges and tangerines, pomegranate, peach, Lee, apricot, pears, apple, tomato, watermelon are a kind of omnivorousness and destructive quarantine pest insect.
The citrus fruit fly adult lays eggs under host's pericarp, pierces pulp after the larva hatching and causes harm and grow.Maturescent fruit by harm pericarp browning, softening, even rots; The fruit that do is caved in by harm, and impaired development, fruit are subject to damage and subject to other sick worm and invade; That invades late period does not cause shedding, but the back of gathering is in transportation and storage period, and the fruit that is injured is perishable.World many countries is classified it as important quarantine property insect with the area.In China is a kind of inward plant quarantine property pest.
In 3-9 generation, take place in citrus fruit fly 1 year, do not have the strict process of surviving the winter, and each generation is overlapped, takes place irregularly from generation to generation, and various together worm attitudes are also deposited simultaneously, and this brings many difficulties to its integrated control.
The existing Prevention Technique of citrus fruit fly mainly is the basis with cultural control (collection shedding), and physical control (fruit bagging), biological control (trapping male insect) combine with chemical control.The citrus fruit fly attractant that uses at present all is to be main component with methyl eugenol, is a kind of sex attractant, has this attractant of document announcement only to lure the citrus fruit fly male imago, and its female adult worm is not had attractant effect.Traditional chemical pesticide is because citrus fruit fly is laid eggs in pericarp and the food characteristic and control efficiency is undesirable of diving, and poisonous to people and other useful insect, and contaminated environment and insect are prone to develop immunity to drugs.
Summary of the invention
The objective of the invention is to overcome the defective of above-mentioned prior art, provide a kind of citrus fruit fly male imago and female adult worm are all had active high, multivalence protein attractant that the lasting period is long and preparation method thereof and application.
Realize that technical scheme of the present invention is following:
A kind of citrus fruit fly multivalence protein attractant, component is by weight percentage:
Multivalence peptone 5-20%; Brown sugar 5-20%; Vinegar 10-20% or ammonium acetate 1-3%; Preservative (Sodium Benzoate or methyl p-hydroxybenzoate) 0.1-0.3%; Methyl eugenol 0-2%; Insecticide (chlopyrifos or pyrethroid are wherein a kind of for DDT EC, chlorophos) 0.1-1%; Keep the skin wet to the full dose of prescription.
Optimization formula of the present invention each component by weight percentage is following: multivalence peptone 10%; Brown sugar 10%; Ammonium acetate 1.5%; Methyl p-hydroxybenzoate 0.15%; Methyl eugenol 0.5%; Chlorophos 0.5%; Keep the skin wet to full dose.
Described multivalence peptone (available from Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd), brown sugar is the commercially available prod, described vinegar is commercially available light-coloured vinegar or mature vinegar or mature vinegar.Said ammonium acetate (available from Chemical Reagent Co., Ltd., Sinopharm Group), preservative is the commercial goods.Said methyl eugenol (available from sweetening treatment Co., Ltd in the Hangzhou) is the commercial goods, effectively purity＞95%.Said insecticide is that (content is 80% to the commercially available agricultural chemical DDT EC; Available from the biochemical Industrial Co., Ltd of Henan Province's Planck); Chlorophos (available from the Nanning chemical inc), chlopyrifos (available from the grand chemical industry of Jiangsu scape Co., Ltd), pyrethroid (available from the Henan Province eastern bright chemical industry Co., Ltd that breaks a seal).
Citrus fruit fly multivalence protein attractant provided by the present invention is main with the multivalence peptone, mixes with ammonium acetate, methyl eugenol, and it is simple to have production technology, low cost and other advantages; Not only good to the male worm trapping effect, and female worm also had good attractant effect.
Embodiment
Through embodiment the present invention is further described, embodiment should not be used as limitation of the present invention.
Embodiment 1:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone (available from Qingdao GaoKeYuan Hai Bo Bioisystech Co., Ltd product, down together) 5g; Place the 100ml conical flask, add brown sugar 5g, light-coloured vinegar 10ml; Preservative sodium benzoate (available from the prosperous chemical industry of Shanghai well Co., Ltd product, down together) 0.15g, DDT EC 1ml; Add the abundant stirring and dissolving of water 78.85ml and mix, obtain a kind of citrus fruit fly multivalence protein attractant.
Embodiment 2:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g, light-coloured vinegar 15ml, preservative sodium benzoate 0.15g; Chlorophos is (available from the Nanning chemical inc; Down together) 0.5ml adds the abundant stirring and dissolving of water 64.35ml to mix, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 3:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 5g, places the 100ml conical flask; Add brown sugar 5g, light-coloured vinegar 10ml, the preservative methyl p-hydroxybenzoate is (available from the true Bioisystech Co., Ltd in Shanghai product; 0.2ml down together); Chlorophos 0.1ml adds the abundant stirring and dissolving of water 79.7ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 4:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 20g, places the 100ml conical flask; Add brown sugar 5g, ammonium acetate 1 (available from Chemical Reagent Co., Ltd., Sinopharm Group) g, preservative sodium benzoate 0.3g; Methyl eugenol (available from sweetening treatment Co., Ltd product in the Hangzhou, down together) 2ml, chlopyrifos (available from the grand chemical industry of Jiangsu scape Co., Ltd) 0.2ml; Add the abundant stirring and dissolving of water 71.5ml and mix, obtain a kind of citrus fruit fly multivalence protein attractant.
Embodiment 5:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g; Ammonium acetate 1.5g, preservative methyl p-hydroxybenzoate 0.15g, chlorophos 0.5ml; Add the abundant stirring and dissolving of water 77.85ml and mix, obtain a kind of citrus fruit fly multivalence protein attractant.
Embodiment 6:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g, ammonium acetate 3g, preservative sodium benzoate 0.1g; Methyl eugenol 0.1ml, pyrethroid (available from the Henan Province eastern bright chemical industry Co., Ltd that breaks a seal, down with) 1ml; Add the abundant stirring and dissolving of water 75.8ml and mix, obtain a kind of citrus fruit fly multivalence protein attractant.
Embodiment 7:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 20g, places the 100ml conical flask; Add brown sugar 5g, ammonium acetate 1g, preservative sodium benzoate 0.2g; Methyl eugenol 2ml; Chlorophos 0.2ml adds the abundant stirring and dissolving of water 71.6ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 8:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 20g, places the 100ml conical flask; Add brown sugar 20g, light-coloured vinegar 15ml, preservative methyl p-hydroxybenzoate 0.2g; Methyl eugenol 0.5ml; Pyrethroid 0.2ml adds the abundant stirring and dissolving of water 44.1ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 9:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 15g, places the 100ml conical flask; Add brown sugar 10g, light-coloured vinegar 20ml, preservative methyl p-hydroxybenzoate 0.3g; Methyl eugenol 0.5ml; Chlopyrifos 0.5ml adds the abundant stirring and dissolving of water 53.7ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 10:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g, light-coloured vinegar 15ml, preservative methyl p-hydroxybenzoate 0.15g; Methyl eugenol 0.5ml; Chlorophos 0.5ml adds the abundant stirring and dissolving of water 63.85ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 11:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g, ammonium acetate 3g, preservative sodium benzoate 0.15g; Methyl eugenol 0.1ml, DDT EC (content is 80%, available from the biochemical Industrial Co., Ltd of Henan Province's Planck) 1ml; Add the abundant stirring and dissolving of water 75.75ml and mix, obtain a kind of citrus fruit fly multivalence protein attractant.
Embodiment 12:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 15g, places the 100ml conical flask; Add brown sugar 10g, light-coloured vinegar 20ml, preservative methyl p-hydroxybenzoate 0.15g; Methyl eugenol 0.5ml; Chlorophos 0.5ml adds the abundant stirring and dissolving of water 53.85ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 13:
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 15g, ammonium acetate 1.5g, preservative sodium benzoate 0.15g; Methyl eugenol 1ml; Chlorophos 0.1ml adds the abundant stirring and dissolving of water 72.25ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 14
The citrus fruit fly attractant of preparation 100ml takes by weighing multivalence peptone 10g, places the 100ml conical flask; Add brown sugar 10g, ammonium acetate 1.5g, preservative methyl p-hydroxybenzoate 0.15g; Methyl eugenol 0.5ml; Chlorophos 0.5ml adds the abundant stirring and dissolving of water 77.35ml and mixes, and obtains a kind of citrus fruit fly multivalence protein attractant.
Embodiment 15: the application implementation example
The attractant of getting embodiment 1-14 preparation respectively is added drop-wise to process in the cotton core and lures core, is divided in the Steiner trapper (Entry-Exit Inspection and Quarantine Bureau provides by GuangZhou, China) each trapper attractant 4ml of the present invention that packs into.Other gets methyl eugenol (contrast 1) and contrast 2 (sugar-vinegar liquid) each 4ml, is added drop-wise to lure in the core, as contrast.Described trapper is placed the bigger guava garden of citrus fruit fly insect density.Trapper overhead highly is about 1.5 meters.The spacing of each trapper is a 30-50 rice.Each handles 3 repetitions.Respectively at 1 day, 3 days, 5 days, 7 days mensuration insect-catching effects.
Table 1 attractant of the present invention and methyl eugenol compare the attractant effect of citrus fruit fly
Show that by table 1 field trapping experiment result different embodiment more of the present invention have attractant effect preferably to citrus fruit fly male and female adult; The trap effect of the attractant of embodiment 5,10,14 preparations is respectively 2.4 times, 1.8 times and 4.0 times of contrast 1 methyl eugenol, and the trap effect of the attractant of embodiment 2,5,10,14 preparations is respectively 2.1 times, 5.6 times, 4.1 times, 9.2 times of contrast 2 sugar-vinegar liquids.And the embodiment of the invention is the male and female double plays, and the contrast methyl eugenol is only effective to male worm.The harm because the female worm of citrus fruit fly lays eggs, therefore attractant of the present invention has the more real meaning of preventing and treating to the female worm of citrus fruit fly.
Claims (4)
1. multivalence protein attractant that is applicable to citrus fruit fly, it is characterized in that: each component by weight percentage is following:
Multivalence peptone 5-20%;
Brown sugar 5-20%;
Vinegar 10-20% or ammonium acetate 1-3%;
Preservative 0.1-0.3%;
Methyl eugenol 0-2%;
Insecticide 0.1-1%;
Add water to the full dose of prescription;
Wherein: preservative is Sodium Benzoate or methyl p-hydroxybenzoate; Described insecticide is a DDT EC, chlorophos, chlopyrifos or pyrethroid.
2. attractant according to claim 1 is characterized in that, each component by weight percentage is following:
Multivalence peptone 10%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Methyl eugenol 0.5%;
Chlorophos 0.5%;
Add water to the full dose of prescription.
3. preparation method who is applicable to citrus fruit fly multivalence protein attractant, its step is following:
Each component by weight percentage is following: multivalence peptone 5-20%; Brown sugar 5-20%; Vinegar 10-20% or ammonium acetate 1-3%; Preservative 0.1-0.3%; Methyl eugenol 0-2%; Insecticide 0.1-1%, wherein said preservative are Sodium Benzoate or methyl p-hydroxybenzoate, and described insecticide is a DDT EC; Chlorophos; Chlopyrifos or pyrethroid, add water to the full dose of prescription after, fully stirring and dissolving mixes the multivalence protein attractant that is citrus fruit fly.
4. method according to claim 2 is characterized in that, each component by weight percentage is following:
Multivalence peptone 10%;
Brown sugar 10%;
Ammonium acetate 1.5%;
Methyl p-hydroxybenzoate 0.15%;
Methyl eugenol 0.5%;
Chlorophos 0.5%;
Add water to the full dose of prescription.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010105368581A CN102461563A (en) | 2010-10-30 | 2010-10-30 | Polyvalent protein attractant suitable for bactroceradorsalis (Hendel), its preparation method and application |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102754665A (en) * | 2012-07-17 | 2012-10-31 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
| CN103283724A (en) * | 2013-06-17 | 2013-09-11 | 贵州省亚热带作物研究所 | Fruit fly trapping agent, preparation method and application thereof |
| CN103535386A (en) * | 2012-07-16 | 2014-01-29 | 铜川市园艺工作站 | Special-purpose concentrated insect-inducing liquid for drosophila melanogaster as well as preparation method thereof |
| CN104542720A (en) * | 2014-12-19 | 2015-04-29 | 邓仁双 | Method for biologically controlling Bactrocera minax (Enderlein) |
| CN104892090A (en) * | 2015-04-30 | 2015-09-09 | 河北双星种业有限公司 | Multifunctional insecticidal and antibacterial nutrient solution and use method thereof |
| CN105010440A (en) * | 2015-08-20 | 2015-11-04 | 四川新致高生物科技有限公司 | Tephritidae insect pheromone attractant and trap-killing agent adopting attractant |
| CN105309432A (en) * | 2014-10-26 | 2016-02-10 | 仲恺农业工程学院 | Bactrocera dorsalis attractant |
| CN108323532A (en) * | 2018-02-13 | 2018-07-27 | 四川省农业科学院植物保护研究所 | A kind of slow-release citrus fruit fly attractant and production method |
| CN109452325A (en) * | 2018-12-06 | 2019-03-12 | 葛诚 | A kind of dacus dorsalis male and female are double to lure attractant |
| CN110150009A (en) * | 2019-05-29 | 2019-08-23 | 广西壮族自治区农业科学院 | A kind of method that Synthetical prevention irrigates mandarin orange orchard citrus fruit fly and wood louse |
| CN112602706A (en) * | 2021-01-09 | 2021-04-06 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN113365495A (en) * | 2018-12-05 | 2021-09-07 | 蒂克斯迪克有限责任公司 | Tick trap and method of trapping ticks |
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| CN101589728A (en) * | 2009-06-30 | 2009-12-02 | 福建农林大学 | Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof |
| CN101690478A (en) * | 2009-10-15 | 2010-04-07 | 杜进平 | Food-sex attraction slow-release attractant for bactrocera dorsalis, and application thereof |
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Patent Citations (3)
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| CN1793233A (en) * | 2005-12-27 | 2006-06-28 | 云南农业大学 | Attractant for juxiaoshi fly |
| CN101589728A (en) * | 2009-06-30 | 2009-12-02 | 福建农林大学 | Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof |
| CN101690478A (en) * | 2009-10-15 | 2010-04-07 | 杜进平 | Food-sex attraction slow-release attractant for bactrocera dorsalis, and application thereof |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535386A (en) * | 2012-07-16 | 2014-01-29 | 铜川市园艺工作站 | Special-purpose concentrated insect-inducing liquid for drosophila melanogaster as well as preparation method thereof |
| CN102754665B (en) * | 2012-07-17 | 2014-05-14 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
| CN102754665A (en) * | 2012-07-17 | 2012-10-31 | 华中农业大学 | Application of hansenula anomala in preparing citrus fruit fly bait |
| CN103283724A (en) * | 2013-06-17 | 2013-09-11 | 贵州省亚热带作物研究所 | Fruit fly trapping agent, preparation method and application thereof |
| CN105309432B (en) * | 2014-10-26 | 2017-07-28 | 仲恺农业工程学院 | Attractant for juxiaoshi fly |
| CN105309432A (en) * | 2014-10-26 | 2016-02-10 | 仲恺农业工程学院 | Bactrocera dorsalis attractant |
| CN104542720A (en) * | 2014-12-19 | 2015-04-29 | 邓仁双 | Method for biologically controlling Bactrocera minax (Enderlein) |
| CN104892090A (en) * | 2015-04-30 | 2015-09-09 | 河北双星种业有限公司 | Multifunctional insecticidal and antibacterial nutrient solution and use method thereof |
| CN105010440A (en) * | 2015-08-20 | 2015-11-04 | 四川新致高生物科技有限公司 | Tephritidae insect pheromone attractant and trap-killing agent adopting attractant |
| CN108323532A (en) * | 2018-02-13 | 2018-07-27 | 四川省农业科学院植物保护研究所 | A kind of slow-release citrus fruit fly attractant and production method |
| CN113365495A (en) * | 2018-12-05 | 2021-09-07 | 蒂克斯迪克有限责任公司 | Tick trap and method of trapping ticks |
| CN109452325A (en) * | 2018-12-06 | 2019-03-12 | 葛诚 | A kind of dacus dorsalis male and female are double to lure attractant |
| CN110150009A (en) * | 2019-05-29 | 2019-08-23 | 广西壮族自治区农业科学院 | A kind of method that Synthetical prevention irrigates mandarin orange orchard citrus fruit fly and wood louse |
| CN112602706A (en) * | 2021-01-09 | 2021-04-06 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
| CN112602706B (en) * | 2021-01-09 | 2022-02-01 | 福建农林大学 | Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof |
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Application publication date: 20120523 |