CN106386851A - Attractant and method for trapping mosquitoes - Google Patents

Attractant and method for trapping mosquitoes Download PDF

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Publication number
CN106386851A
CN106386851A CN201610784153.9A CN201610784153A CN106386851A CN 106386851 A CN106386851 A CN 106386851A CN 201610784153 A CN201610784153 A CN 201610784153A CN 106386851 A CN106386851 A CN 106386851A
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attractant
trimethylamine
carbon dioxide
mosquito
trap
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CN106386851B (en
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彭雨旸
彭新辉
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/04Carbon disulfide; Carbon monoxide; Carbon dioxide
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02Amines; Quaternary ammonium compounds
    • A01N33/04Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an attractant and method for trapping mosquitoes. Main functional components of the attractant comprise carbon dioxide and trimethylamine. The method for trapping the mosquitoes, disclosed by the invention, is simple and scientific, the effect is obvious and reliable, raw materials are easily obtained, and the manufacturing is convenient, economical, environmentally friendly and safe, so that the marketing value is great.

Description

A kind of attractant of trapping mosquitoes and method
Technical field
The present invention relates to a kind of attractant of trapping mosquitoes and method.
Background technology
Mosquito belongs to Insecta, Diptera, Dulicidae, and the whole world there are about 2700 kinds.China can pathophorous mosquito substantially can divide For three classes:One class is anopheles, popular name malarial mosquito, main propagation malaria;Another kind of cry culex, main propagation filaricide and popular second Type encephalitis;3rd class, yellow-fever mosquito, has black and white speckle with it, is black speck mosquito, the main propagation B-mode and dengue fever of popularity again.These The whole world is caused per year over megadeath by the disease of mosquitoes spread.Additionally, biting of mosquito also brings to human lives Have a strong impact on, such as bite rear red swelling of the skin very itch, drone sound easily affect sleep.
The all one's life of mosquito needs to experience ovum, larva, pupa, adult four-stage, is belonging to complete metamorphosiss biological.Mosquito is divided into Female mosquito and male mosquito, wherein male mosquito is only sucked nectar and can be survived with water, and female mosquito needs to bite animal and sucks blood Promote the maturation of internal ovum thus completing reproductive process, therefore the mosquito stinging people belongs to female mosquito.
Because mosquito has a strong impact on to the mankind, since the last century, people design a lot of ways to eliminate mosquito, wherein Most importantly use chemical insecticide (as pyrethrin pesticide, DDT etc.), but these pesticide life-time service are not only to health It is harmful to, pollutes environment it is often more important that making mosquito develop immunity to drugs and so that insecticide efficiency is reduced.Mosquito incense using chemical insecticide Fumigate and be also only capable of temporary transient expelling mosquito and its quantity can not be reduced.
In view of the foregoing, people increase the habits such as phototaxis using mosquito, chemotactic odor property and carry out non-killing The research of mosquito is trapped and killed in worm agent mosquito eradication.Such as, Nasci of nineteen eighty-three etc. reports photo-induction mosquito catcher and utilizes ultraviolet light for these researchs To attract mosquito, but male mosquito can only be captured, and there is no positive effect to female mosquito, particularly to infect dengue fever Aedes aegypti with Aedes albopictus is all invalid.In recent years, Cook et al. (2011), Kline et al. (2012) etc. further study dioxy Change carbon and chemical odor to the epigamic of mosquito it is believed that carbon dioxide is mosquito find target of attack signal of interest material it One.Guo Jia (Guangming Daily/2003 year 07 month 04 day) writes articles and points out, Pfansteihl, acetone and dimethyl disulfide have relatively to mosquito Strong attraction.Additionally, also document report, 1-OCOL, aldehyde C-9 also have very strong attracting action to mosquito.
Although there being so many people to carry out research extensively and profoundly for trapping mosquitoes, these researchs remain many defects Or it is not enough.These document great majority are using single CO2This colorless and odorless gaseous material is as attractant, or adopts Known odoring substance (as matsutake alcohol, acetone, some organic acid including lactic acid, hexanal etc.) combines and makees attractant Carry out trapping mosquitoes.The toxicity that these materials have is larger (as acetone), and some attractant effects are bad, the preparation cost of some attractants High.Further, such as utilize CO2For the document of attractant, mostly need electricity auxiliary, this adds increased use cost, there is no the area of electricity Domain cannot use, thus limiting its range;High temperature is needed to produce carbon dioxide, very inconvenient.For luring It should simplification as far as possible for catching mosquito, could preferably promote indoors or outdoor all can be applied.
Content of the invention
Present invention solves the technical problem that being, improve the attractant effect to mosquito, it is a kind of good to mosquito attractant effect to provide Attractant and trapping mosquitoes simple to operation method.
The technical scheme is that, a kind of attractant of trapping mosquitoes, the main functional component of described attractant are provided Including carbon dioxide and trimethylamine.
Further, the main functional component of described attractant also includes 1-OCOL.
Further, the main functional component of described attractant also includes aldehyde C-9.
Further, the main functional component of described attractant is made up of carbon dioxide and trimethylamine.
The present invention further provides a kind of method of trapping mosquitoes, separately puts in the trap of at least one opening Put the raw material that can discharge carbon dioxide, trimethylamine respectively.
Further, at the opening of described trap it is black, or the generally black of described trap.
Further, the carbon dioxide volumetric concentration at the opening of described trap is 3-6%.
Further, the raw material of described releasable trimethylamine is the aqueous solution of trimethylamine, and in aqueous solution, trimethylamine content is 0.0001~15 μ g/L, preferably 0.01~3 μ g/L, more preferably 0.1~1.5 μ g/L.The aqueous solution of trimethylamine is not only permissible Release trimethylamine, but also steam can be discharged, improve the humidity of air it is also possible to attract mosquito.
Further, the carbon dioxide in described trap is by dry ice sublimation, bicarbonate and acid reaction, sugared and micro- life At least one that thing ferments in three kinds of approach produces;The fermentation approach preferably employing sugar with microorganism produces.
Further, the microorganism that described employing fermentation approach produces carbon dioxide is yeast.
Further, add penicillin in described fermentation raw material, make penicillin account for the 0.05% of total fermentation raw material quality ~0.15%.
Further, described fermentation raw material water dissolution or moistening when, the pH value of aqueous solution is 3~7.5, preferably 4.5 ~5.
Further, when described fermentation approach produces carbon dioxide, partly sugar carries out slow release process with shitosan.Sugar is entered It is in order to carbon dioxide when reducing initial produces speed that row slow release is processed, and extends the time that fermentation produces carbon dioxide.
Further, the described bicarbonate producing carbon dioxide with acid reaction be sodium bicarbonate, in potassium bicarbonate extremely Few one kind.
Further, the described acid reacting generation carbon dioxide with bicarbonate is one of mineral acid or organic acid, Mineral acid is dilute hydrochloric acid or dilute sulfuric acid;Organic acid is citric acid, malic acid, oxalic acid, at least one in tartaric acid, preferably lemon Lemon acid.
The principle of the present invention is:The clear and definite carbon dioxide of prior art is the signal of interest material that mosquito finds target of attack One of;And trimethylamine is as carbon dioxide, during low concentration, mosquito has special sensitivity to it.The present invention by trimethylamine with Carbon dioxide combines as attractant, finds that this ratio of gas mixture any individually gas all more solution attracts mosquito, body Reveal and obviously worked in coordination with facilitation.By two kinds of combination of gases of trimethylamine of carbon dioxide and suitable concn are risen Come so that it may be attracted in trapping " trap " device mosquito thus reaching trapping purpose.
The carbon dioxide of the present invention can be by dry ice, bicarbonate and acid reaction, sugar and three kinds of approach of fermentable At least one generation.Because the carbon dioxide release of dry ice approach, bicarbonate and acid reaction approach is too fast, the persistent period exists Problem, therefore the fermentation approach preferably employing sugar with microorganism produces.Send out by aerobic under aqueous solution or wet condition with yeast for sugar Ferment, just can persistently produce carbon dioxide naturally.Process conditions during fermentation have:Sugar can using monosaccharide (referring to glucose or Fructose) or Disaccharidase (sucrose, maltose), it is preferred to use one of glucose in monosaccharide or Fructose or two kinds.Because yeast merely with Glucose, Fructose, additional disaccharidase also will by dissolving or moistening when the water that adds or the water producing during fermentation make it be degraded to Portugal Grape sugar, Fructose could be decomposed generation CO by yeast2.Add during water, pH value is adjusted to 3~7.5, preferably 4.5 with acid in advance~ 5;Add water when, in water can plus also can not added with antibiotic, be preferably added to the antibiotic (penicillins) of bacteria growing inhibiting.In order to Extend the time that fermentation produces carbon dioxide, the sugar that part adds first carries out slow release process.These conditions meet yeast and send out The optimal conditionss of ferment, can suppress antibacterial and fungus growth in fermentation raw material by antibiotic and acid adding simultaneously again, not interfere with Yeast fermentation.
Described trimethylamine derives from commercially available trimethylamine aqueous solution, is diluted to very low suitable concn during use.Low dense When spending, people will not be clearly felt that its stink, therefore it can indoors or outdoor carries out trapping mosquitoes use.
The attractant effusion mouth region of the trap of the present invention has following feature:There is the mouth of two insertions, wherein close The open area in container body area is less than the open area away from container body area;It is shaped as one of inverted round stage, inverted trapezoidal.Pass through The design that this outlet bore incrementally increases, makes attractant gas (the carbon dioxide and trimethylamine) temperature of effusion be increased to and more connect Nearly body temperature, to improve the effect of attracting mosquitoes.
It is separated in trap with the raw material area containing trimethylamine that carbon dioxide produces area.This design is Because trimethylamine is alkaline matter, fermentation zone is acid condition, and both can not mix.
Trap also has following feature:Attractant effusion mouth region or device generally black, preferred embodiment is generally Black;Insulation construction is carried outside container body area.Trap is set to black and is because mosquito interesting black habit;To hold Device is provided with insulation construction outside body region, is the optimal temperature conditions (20~30 DEG C about) both having ensured yeast fermentation, and energy Improve the temperature of attractant, thus increasing the allure to mosquito.So, the temperature in whole trap is preferably 20~30 ℃.
It should be noted that the concentration of Carbon Dioxide in Air is about 0.03%, human normal exhaled gas carbon dioxide Concentration is about 4% about, and with momental increasing, the concentration of the carbon dioxide in exhaled gas also can raise;Different The gas concentration lwevel of people's exhalation is also different.Mosquito is to apparently higher than atmospheric concentration until breathe out the titanium dioxide of concentration close to people Carbon all has different degrees of tropism.Compared with the prior art, the beneficial effects are mainly as follows:
1. carbon dioxide and trimethylamine both are had the effumability thing of stronger attracting action by the present invention first to mosquito Matter is combined into attractant, it is to avoid the defect of document or deficiency described in aforementioned background art, thus effectively increasing trapping mosquito The effect of son.
2. the present invention to be ensured yeast fecund, to be produced carbon dioxide for a long time using preferably fermentation condition.This Invention preferably employs the conditions such as glucose, at least one monosaccharide in Fructose and preferred pH, and to can guarantee that yeast fermentation has more excellent Condition;Additionally, the present invention adds the antibiotic (as penicillins) of bacteria growing inhibiting also in fermentation raw material, by adjusting The acid such as propanoic acid that pH value adds can suppress fungus growth again, thus preferably avoiding antibacterial with mycete to yeast-leavened interference, Ensure the aerogenesis steady in a long-term of yeast.Additionally, partly sugar is carried out slow release process by the present invention in advance, the supply time of sugar prolongs Long, thus the yeast fermentation uniform aerogenesis time can be extended, also avoid need to frequently change during fermentation and produce carbon dioxide raw material Drawback.
3. the present invention makes simple science, and device is easy to get without driven by power, raw material, macroeconomic environmental protection.The present invention's Carbon dioxide is produced with yeast fermentation by sugar, and sugar can be obtained by the D/W of injection or commercial glucose reagent, Yeast is i.e. commercially available in supermarket, and single 15 grams of active dry yeasts of bag are only 2 yuan about;The acquisition of trimethylamine is also easy to, can Directly buy ready-made trimethylamine aqueous solution.The raw material of trap is also very simple and easy to get with making, and container body area can adopt city Top half is cut off formation gas effusion area, lower half during making by the large volume edible vegetable oil sold, beverage, pure water sky kettle Part forms container body area;Place product carbon dioxide raw material and trimethylamine raw material, Ran Hou in container body area bottom respectively Empty kettle obtains two-part respective clip and uses double faced adhesive tape adhesive plaster glue one circle respectively after cutting off, then had leisure kettle top half is inverted In the kettle body of lower half, mouth is taken in the sealing of blend compounds cloth, makes heat-insulation layer in kettle body outer layer with Cotton Gossypii, then uses black plastic bag bag Wrap up in trap.Because there being heat-insulation layer, yeast fermentation can produce heat to the present invention, along with escape orifice inversion heat up with The higher temperature in summer itself, just can make emergent gass temperature closer to body temperature, therefore not need needed for aforementioned documents Electric heater unit, thus expand its range.
In a word, the invention provides a kind of new with the attractant of trapping mosquitoes, and can defining according to this attractant A kind of method of trapping mosquitoes, trapping mosquitoes methodological science of the present invention, raw material obtains easily, makes simple and convenient, economic and environment-friendly Safety, effect is reliable, has wide market popularization value.
Specific embodiment
Embodiment and comparative example
First, the contrast test of different attractants:
The fermentation liquid of carbon dioxide is produced in preparation, and method is:Weigh 40 grams of glucoses, be dissolved in the third acid for adjusting pH value to 4.2 250 milliliters of aqueous solutions in, add 1.5 grams of certain brand high activity dried yeast, stir evenly.
The manufacture method of trap:The plastics sky kettle containing certain 5 liters of pure water of brand is cut off as upper and lower two Point, two-part respective clip is enclosed with double faced adhesive tape adhesive plaster glue one respectively, then by gained oil can top half be inverted be fixed under Partly go up, blend compounds cloth sealing take mouth, make heat-insulation layer in kettle body outer layer with Cotton Gossypii, then with black plastic bag parcel trapping Device.
The contrast test carrying out different attractant composition trapping mosquitoes is specific as follows:
The attractant of each comparative example forms:
Comparative example 1:Only with the produced carbon dioxide of the above-mentioned fermentation liquid preparing as attractant.Another one small beaker is put Enter 10 milliliters of pure water.
Comparative example 2:With the produced carbon dioxide of the above-mentioned fermentation liquid preparing as a kind of attractant composition, also one kind is drawn By 10 milliliters of mass fraction adding in another one small beaker, the aqueous solution of the acetone by 0.5% is volatilized lure composition Acetone.
Comparative example 3:With the produced carbon dioxide of the above-mentioned fermentation liquid preparing as a kind of attractant composition, also one kind is drawn By 10 milliliters of mass fraction adding in another one small beaker, newborn aqueous acid by 0.5% is volatilized lure composition Lactic acid.
Comparative example 4:The 1.5 μ g/L trimethylamine aqueous solutions that attractant composition adds 10 milliliters by a small beaker are volatilized Trimethylamine;Another one beaker loads 291.5 grams of water.
Embodiment 1:With the produced carbon dioxide of the above-mentioned fermentation liquid preparing as a kind of attractant composition, also one kind is drawn The trimethylamine that lure composition is volatilized by 10 milliliters of the 1.5 μ g/L trimethylamine aqueous solutions adding in another one small beaker.
Embodiment 2:Have 4 kinds of compositions and be combined into attractant, be the produced carbon dioxide of the above-mentioned fermentation liquid preparing respectively Trimethylamine that 10 milliliters of the 1.5 μ g/L trimethylamine aqueous solutions adding in composition, another one small beaker are volatilized, also has one 10 milliliters of 0.05% 1-OCOLs containing mass fraction adding in small beaker, 0.04% aldehyde C-9 of mass fraction 1-OCOL and aldehyde C-9 that ethanol water is volatilized.
The specific implementation process of trapping mosquitoes is:
Respectively the attractant of above-mentioned each comparative example and embodiment is put in respective trap, after the sun sets, hang In liftoff 50 centimetres certain cell woods, it is separated by 15 meters, at day fetches within second day, and open in small-sized mosquito net and lure Device for catching is suitable for reading, and the mosquito of trapping is counted.Next re-replaces random exchange placement location after attractant at dusk, continuously Carry out 7 days, finally count the trapping mosquitoes quantity of each comparative example and embodiment.
Each comparative example lures mosquito quantity as follows with embodiment:
Test brief summary:
Above contrast experiment shows, above-mentioned each trapping bait (combination) all has trap effect to mosquito, but have each other necessarily poor Different, wherein, individually using carbon dioxide as the trapping mosquitoes minimum number of trapping bait composition, and with embodiment 2 (carbon dioxide, Trimethylamine, aldehyde C-9, the combination of 1-OCOL) trapping mosquitoes quantity most;Embodiment 1 (trimethylamine and carbon dioxide group Close) trapping mosquitoes quantity time many, and than individually with the comparative example 1 of carbon dioxide and independent with trimethylamine for attractant composition Comparative example 4 lures mosquito quantity to increase by 125%, 46.94% respectively every night.
2nd, in attractant the more excellent concentration of trimethylamine determination
According to the method for above-described embodiment, have carbon dioxide as one of attractant composition in the case of (use certain brand When gas concentration lwevel detector records that attractant is just thrown in every night, each trap gas escape port gas concentration lwevel is 3.5 ± 0.4%), carried out trimethylamine different content further lures mosquito Comparision Test.
The above results show, in the range of 0.001~1.5 μ g/L, with the increase of trimethylamine content, the trapping of attractant Mosquito populations integrally assume increase trend, wherein most with the trimethylamine content trapping mosquitoes quantity of 1.5 μ g/L;The three of 15 μ g/L It is probably that trimethylamine content is too high that methylamine content is processed, and the attractant effect of mosquito is deteriorated on the contrary.As can be seen here, three in attractant The more excellent concentration range of methylamine is 0.1~1.5 μ g/L.

Claims (10)

1. a kind of attractant of trapping mosquitoes it is characterised in that the main functional component of described attractant include carbon dioxide and Trimethylamine.
2. attractant as claimed in claim 1 it is characterised in that the main functional component of described attractant also to include 1- pungent Alkene -3- alcohol.
3. attractant as claimed in claim 1 or 2 is it is characterised in that the main functional component of described attractant also includes nonyl Aldehyde.
4. attractant as claimed in claim 1 it is characterised in that the main functional component of described attractant by carbon dioxide and Trimethylamine forms.
5. a kind of method of trapping mosquitoes is it is characterised in that separated in the trap of at least one opening distinguish Release carbon dioxide, the raw material of trimethylamine.
6. method as claimed in claim 5 is it is characterised in that be black at the opening of described trap, or described lures The generally black of device for catching.
7. method as claimed in claim 5 is it is characterised in that carbon dioxide volumetric concentration at the opening of described trap For 3-6%.
8. method as claimed in claim 5 is it is characterised in that the raw material of described releasable trimethylamine is the water-soluble of trimethylamine Liquid, in aqueous solution, trimethylamine content is 0.0001~15 μ g/L.
9. method as claimed in claim 8 it is characterised in that in described aqueous solution trimethylamine content be 0.1~1.5 μ g/L.
10. method as claimed in claim 5 is it is characterised in that the carbon dioxide in described trap is by dry ice sublimation, carbon Sour hydrogen salt is produced with acid reaction, sugar and at least one in three kinds of approach of fermentable.
CN201610784153.9A 2016-08-31 2016-08-31 The attractant and method of a kind of trapping mosquitoes Expired - Fee Related CN106386851B (en)

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CN111838191A (en) * 2019-04-25 2020-10-30 佛山市格林盈璐电器科技有限公司 Novel bionic mosquito attractant and preparation method thereof
CN114375954A (en) * 2022-03-01 2022-04-22 广东省科学院动物研究所 Binding pyruvic acid to adsorb CO by using chitosan as carrier2Mosquito trapping composition and preparation method and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362674A (en) * 2018-11-30 2019-02-22 山东大学 A kind of carbon dioxide and lactic acid independent generator and portable mosquito catching-killing device
CN111838191A (en) * 2019-04-25 2020-10-30 佛山市格林盈璐电器科技有限公司 Novel bionic mosquito attractant and preparation method thereof
CN114375954A (en) * 2022-03-01 2022-04-22 广东省科学院动物研究所 Binding pyruvic acid to adsorb CO by using chitosan as carrier2Mosquito trapping composition and preparation method and application thereof

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