CN100569080C - The compounded pesticides of paecilomyces fumosoroseus and Imidacloprid - Google Patents
The compounded pesticides of paecilomyces fumosoroseus and Imidacloprid Download PDFInfo
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- CN100569080C CN100569080C CNB2007100315139A CN200710031513A CN100569080C CN 100569080 C CN100569080 C CN 100569080C CN B2007100315139 A CNB2007100315139 A CN B2007100315139A CN 200710031513 A CN200710031513 A CN 200710031513A CN 100569080 C CN100569080 C CN 100569080C
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- paecilomyces fumosoroseus
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Abstract
The invention discloses the compounded pesticides of a kind of paecilomyces fumosoroseus and Imidacloprid.It is made up of host and assistant agent, and host is 5～50% by mass percentage, and assistant agent is 50～95%; Host is the mixture of paecilomyces fumosoroseus and Imidacloprid, paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 5～50; Described assistant agent is stabilizing agent, auxiliary agent and filler, the invention discloses to use made wettable powder for fungi spore, fungal spore suspending agent and the water dispersant of above-mentioned bacterial strains.Through long-term biological study and the indoor bioassay of infecting, paecilomyces fumosoroseus has the very strong insecticidal effect that infects to various pests especially sucking pest.The compounded pesticides of this paecilomyces fumosoroseus and Imidacloprid has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Description
Technical field
The present invention relates to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Imidacloprid especially.
Background technology
Paecilomyces fumosoroseus (Paecilomyces fumosoroseus (Wize) Brown et Smith) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use paecilomyces fumosoroseus has important economic implications.Paecilomyces fumosoroseus distributes extensively, aphid, Bemisia tabaci, diamond-back moth etc. is had good control efficiency, has a good application prospect.
Imidacloprid (imidacloprid) is widely used efficient, interior suction at present, broad spectrum type nicotinic insecticide, is mainly used in disguised insects such as suckings pest such as preventing and treating aleyrodid, aphid and liriomyza bryoniae.Along with using historical prolongation, insect has been developed into a major issue to the pesticide resistance of Imidacloprid.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the Compositional type insecticide of exploitation Imidacloprid is significant.Still find no at present the relevant paecilomyces fumosoroseus and the compounded pesticides of Imidacloprid.
Summary of the invention
In order to overcome the deficiency that above-mentioned prior art exists, the object of the present invention is to provide a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Imidacloprid.
Purpose of the present invention realizes by following technical proposals: the compounded pesticides of a kind of paecilomyces fumosoroseus and Imidacloprid, form by host and assistant agent, and host is 5～50% by mass percentage, assistant agent is 50～95%; Described host is the mixture of paecilomyces fumosoroseus and Imidacloprid, described paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 5～50; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium (NNO); Described filler is talcum powder, potter's clay or clay.
Described paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown et Smith bacterial strain PF01-N10, belong to hyphomycetales, paecilomyces, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, the bacterial strain preserving number: CCTCC No:M on June 27th, 207088,2007 is in China's typical culture collection center preservation.The Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form verticil mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little pale red, 3～4 * 1～2 μ m, no chlamydospore.
In order to realize the present invention better, described paecilomyces fumosoroseus: the mass ratio of Imidacloprid is 100: 6～25.
As a kind of preferred, described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
Described stabilizing agent, auxiliary agent and filler by mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
The compounded pesticides of described paecilomyces fumosoroseus and Imidacloprid can be processed into formulations such as wetting powder, suspending agent or water dispersant.
The present invention compared with prior art has following advantage and beneficial effect: the Bemisia tabaci polypide that (1) this bacterial strain is infected from the area, GuangZhou, Guangdong Province separates acquisition, is China domestic bacterial strain, is not from external introduction, can adapt to local natural environment.(2) adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) its control efficiency is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, paecilomyces fumosoroseus has the very strong insecticidal effect that infects to various pests especially sucking pest.The compounded pesticides of this paecilomyces fumosoroseus and Imidacloprid has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Following percentage is mass percent, and the Paecilomyces Fumosoroseus strain is PF01-N10, the bacterial strain preserving number: CCTCC No:M 207088, preservation date on June 27th, 2007.
Embodiment 1:10% Paecilomyces varioti imidacloprid suspending agent (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidial suspension: 20% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid (commercial) 3.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 10% Paecilomyces varioti imidacloprid suspending agent, paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 15.The virulence test result is as shown in table 1.
Embodiment 2:5% Paecilomyces varioti imidacloprid suspending agent (diluting 1000 times)
A partly is 10% paecilomyces fumosoroseus conidial suspension: 10% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid 5.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 5% Paecilomyces varioti imidacloprid suspending agent, and described paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 50.The virulence test result is as shown in table 1.
Embodiment 3:50% Paecilomyces varioti imidacloprid wettable powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, 10%WP Imidacloprid 3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti imidacloprid wettable powder.Wherein, Paecilomyces Fumosoroseus powder: the Imidacloprid mass ratio is 100: 6.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces varioti imidacloprid wettable powder
Paecilomyces Fumosoroseus powder (10
10/ g) 50%, Imidacloprid 5%, dodecyl sodium sulfate 14%, Tween-80 10% mixes, and adds clay then and mends to 100%, makes 50% paecilomyces fumosoroseus imidacloprid wettable powder.Wherein, Paecilomyces Fumosoroseus powder: the mass ratio of Imidacloprid is 100: 10.The virulence test result is as shown in table 1.
Embodiment 5:10% Paecilomyces varioti Imidacloprid water dispersant (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidium water dispersant: 20% paecilomyces fumosoroseus conidial powder is dissolved in the glycerine mixed liquor of Tween-80 20% and 10%, and pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid 5.0%, and sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 10% Paecilomyces varioti imidacloprid suspending agent, paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 25.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 paecilomyces fumosoroseus Imidacloprid is to the Toxicity Determination (10 of Bemisia tabaci
3Spore/ml, μ g/ml) (10 is big behind the medicine)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Paecilomyces fumosoroseus | y＝0.34x+3.72 | 0.9617 | 6.11，3.08-12.11 | 100.00 |
| Imidacloprid (behind the medicine 3 days) | y＝1.5799x+3.0199 | 0.9881 | 17.92，16.25-19.76 | |
| Embodiment 1: paecilomyces fumosoroseus: Imidacloprid (100: 15) | y＝0.4251x+3.4930 | 0.9862 | 3.51，0.32-38.58 | 174.23 |
| Embodiment 2: paecilomyces fumosoroseus: Imidacloprid (100: 50) | y＝0.3782x+3.6860 | 0.9856 | 4.49，0.39-52.24 | 135.99 |
| Embodiment 3: paecilomyces fumosoroseus: Imidacloprid (100: 6) | y＝0.4129x+3.6075 | 0.9881 | 2.36，0.17-32.09 | 259.45 |
| Embodiment 4: paecilomyces fumosoroseus: Imidacloprid (100: 10) | y＝0.4038x+3.6579 | 0.9468 | 2.11，0.13-33.38 | 289.57 |
| Embodiment 5: paecilomyces fumosoroseus: Imidacloprid (100: 25) | y＝0.4272x+3.4726 | 0.9593 | 3.57，0.34-41.11 | 171.15 |
R
2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1, the compounded pesticides of a kind of paecilomyces fumosoroseus and Imidacloprid is made up of host and assistant agent, it is characterized in that, host is 5～50% by mass percentage, and assistant agent is 50～95%; Described host is the mixture of paecilomyces fumosoroseus (Paecilomyces fumosoroseus) and Imidacloprid, and described paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 5～50; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium; Described filler is talcum powder, potter's clay or clay; Described Paecilomyces Fumosoroseus strain preserving number: CCTCC NO:M on June 27th, 207088,2007 is in China's typical culture collection center preservation.
2, compounded pesticides according to claim 1 is characterized in that, described paecilomyces fumosoroseus: the Imidacloprid mass ratio is 100: 6～25.
3, compounded pesticides according to claim 1 and 2 is characterized in that: described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
4, compounded pesticides according to claim 1 is characterized in that, described stabilizing agent, auxiliary agent and filler by mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
5, compounded pesticides according to claim 1 and 2 is characterized in that, the compounded pesticides of described paecilomyces fumosoroseus and Imidacloprid is processed into wetting powder, suspending agent or water dispersant.
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| CN101273727B (en) * | 2008-04-22 | 2011-01-19 | 华南农业大学 | Pesticides combined with Paecilomyces fumosoroseus and Acetamiprid |
| CN101273728B (en) * | 2008-04-22 | 2011-01-19 | 华南农业大学 | Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin |
| CN101317581B (en) * | 2008-07-04 | 2011-06-22 | 华南农业大学 | Mixed insecticide of paecilomyces fumosa-roseus brown et smith and avermectin |
| CN101317580B (en) * | 2008-07-04 | 2011-03-23 | 华南农业大学 | Mixed insecticide of paecilomyces fumosa-roseus brown et smith and high-efficiency cypermethrin |
| CN101502270B (en) * | 2009-03-17 | 2011-08-17 | 华南农业大学 | Compositional main pesticide containing Paecilomyces javanicus and Imidacloprid and pesticide |
| CN101502269B (en) * | 2009-03-17 | 2011-08-17 | 华南农业大学 | Compositional main pesticide containing Verticillium lecanii and Acetamiprid and pesticide |
| CN103491787A (en) * | 2010-10-29 | 2014-01-01 | 诺维信生物股份有限公司 | Fungi for odor control |
| CN102630710A (en) * | 2012-04-10 | 2012-08-15 | 江苏省农业科学院 | Method for increasing infectivity of Paecilomyces fumosoroseus on Bemisia tabaci by utilizing plant source extract |
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