CN101513191B - Compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid - Google Patents
Compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid Download PDFInfo
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- CN101513191B CN101513191B CN2009100377454A CN200910037745A CN101513191B CN 101513191 B CN101513191 B CN 101513191B CN 2009100377454 A CN2009100377454 A CN 2009100377454A CN 200910037745 A CN200910037745 A CN 200910037745A CN 101513191 B CN101513191 B CN 101513191B
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Abstract
The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid. The primary agent is a mixture of the verticillium lecanii and the imidacloprid, wherein, the mass ratio between the verticillium lecanii and the imidacloprid is 100: 5-50; while the pesticide consists of a primary agent and a secondary agent, according to mass percent,the primary main agent accounts for 5-50%, and the secondary agent 50-95%. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and imidacloprid of the invention has good effect in preventing andcontrolling pests such as white fly, plant louse, diamond back moth and the like.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant the Verticillium lecanii and the composite desinsection host and the insecticide of Imidacloprid of a kind of environmental protection, pollution-free, noresidue especially.
Background technology
Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas is a kind of insect pathogenic fungus, as a kind of living microorganism agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Verticillium lecanii has important economic implications.Verticillium lecanii distributes extensively, Trialeurodes vaporariorum Westwood, Bemisia tabaci, aphid, thrips etc. is had good control efficiency, has broad application prospects.
Imidacloprid (imidacloprid) is widely used efficient, interior suction at present, broad spectrum type nicotinic insecticide, is used to prevent and treat disguised insects such as sucking pest such as aleyrodid, aphid and liriomyza bryoniae.Along with using historical prolongation, insect strengthens day by day to the pesticide resistance of Imidacloprid.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, therefore, the mixed ratio of screening Verticillium lecanii and Imidacloprid the best, significant.Still find no at present the relevant report of relevant Verticillium lecanii and Imidacloprid compounded pesticides.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, the Verticillium lecanii and the composite desinsection host of Imidacloprid of a kind of environmental protection, pollution-free, noresidue is provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention is achieved through the following technical solutions:
Provide a kind of Verticillium lecanii and Imidacloprid composite desinsection host, described host is the mixture of Verticillium lecanii and Imidacloprid, and the mass ratio of described Verticillium lecanii and Imidacloprid is 100: 5～50.
In order to realize the present invention better, the mass ratio of described Verticillium lecanii and Imidacloprid is preferably 100: 6～and 25.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas bacterial strain VL-N6, belong to hyphomycetales, Verticillium, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, in application number is 200810029163.7 patent application Verticillium lecanii had concrete description.Described Verticillium lecanii bacterium colony is that white is extremely cream-colored, thin cotton-shaped, and the back side is colourless to deep mixed yellow.Conidiophore is undeveloped, like nutrient hypha, Dan Sheng is arranged on it, to the bottle stalk of giving birth to or 3～4 verticillate, and the bottle stalk is very thin, and size is because of bacterial strain and cell age is different differs greatly 8.5-40 * 0.8-2.2 μ m; Conidium is oval to cylindrical, two terminal circle, and Dan Sheng is gathered into head at bottle stalk top, and size makes a variation bigger because of bacterial strain reaches, 2.3-10 * 1-2.6 μ m.
As a kind of preferred, described Verticillium lecanii is Verticillium lecanii bacterium powder or Verticillium lecanii spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 5～50% by mass percentage, and assistant agent is 50～95%.
Described assistant agent is stabilizing agent, auxiliary agent and filler, is respectively by mass percentage that stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 2.5～30%.
Described stabilizing agent is one or more mixtures in glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; One or more mixtures in the preferred Tween-80 of described auxiliary agent, washing powder, dodecyl sodium sulfate, methyl green, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium (NNO); Described filler is kaolin, potter's clay or clay.
The compounded pesticides of described Verticillium lecanii and Imidacloprid can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) the bacterial strain of the present invention Bemisia tabaci polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to domestic natural ecological environment.
(2) the present invention adopts the agricultural chemicals of sub-doses and biopesticide to be mixed, determine to obtain the mixed ratio of good insecticidal effect, reduced the consumption and the persticide residue of Imidacloprid, adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) compounded pesticides control efficiency of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting.The compounded pesticides of Verticillium lecanii and Imidacloprid has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.
Percentage is mass percent in following examples, described Verticillium lecanii is the VL-N6 bacterial strain, the bacterial strain preserving number: CCTCC No:M-208086, preservation date are on June 10th, 2008, specifically describe to see that application number is in 200810029163.7 the patent application.
Embodiment 1:20% Verticillium lecanii imidacloprid oil solution (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 20%, Imidacloprid 3%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Verticillium lecanii imidacloprid oil solution.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Imidacloprid is 100: 15.The virulence test result is as shown in table 1.
Embodiment 2:5% Verticillium lecanii imidacloprid suspending agent
A partly is 10% Verticillium lecanii conidial suspension: 10% Verticillium lecanii conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: Imidacloprid 5.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 5% Verticillium lecanii imidacloprid suspending agent, and described Verticillium lecanii: the Imidacloprid mass ratio is 100: 50.The virulence test result is shown in Table 1.
Embodiment 3:50% Verticillium lecanii imidacloprid wettable powder (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, 10%WP Imidacloprid 3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti imidacloprid wettable powder.Wherein, Verticillium lecanii bacterium powder: the Imidacloprid mass ratio is 100: 6.The virulence test, the result is as shown in table 1.
Embodiment 4:50% Verticillium lecanii imidacloprid oil solution (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, Imidacloprid 2.5%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Verticillium lecanii imidacloprid oil solution.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Imidacloprid is 100: 5.The virulence test result is as shown in table 1.
Embodiment 5:20% Verticillium lecanii imidacloprid oil solution
Verticillium lecanii bacterium powder (10
10Individual/g) 20%, Imidacloprid 5%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Verticillium lecanii imidacloprid oil solution.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Imidacloprid is 100: 25.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Verticillium lecanii Imidacloprid is to the Toxicity Determination of Bemisia tabaci
(10
6Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Verticillium lecanii | y＝0.3744x+3.3240 | 0.9814 | 2.99，0.98-9.16 | 100.00 |
| Imidacloprid (behind the medicine 3 days) | y＝1.5799x+3.0199 | 0.9881 | 17.92，16.25-19.76 | |
| Embodiment 1: Verticillium lecanii: Imidacloprid (100: 15) | y＝0.3392x+3.5571 | 0.9750 | 1.79，0.47-6.74 | 167.04 |
| Embodiment 2: Verticillium lecanii: Imidacloprid (100: 50) | y＝0.3526x+3.4492 | 0.9703 | 2.49，0.74-8.37 | 120.08 |
| Embodiment 3: Verticillium lecanii: Imidacloprid (100: 6) | y＝0.3354x+3.6676 | 0.9645 | 0.93，0.21-42.38 | 321.51 |
| Embodiment 4: Verticillium lecanii: Imidacloprid (100: 5) | y＝0.3211x+3.7648 | 0.9630 | 0.70，0.14-35.97 | 427.14 |
| Embodiment 5: Verticillium lecanii: Imidacloprid (100: 25) | y＝0.3442x+3.5478 | 0.9522 | 1.65，0.43-6.28 | 181.21 |
R
2: determination coefficient; TI: relative virus force index.
Claims (7)
1. a Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) and the composite desinsection host of Imidacloprid, it is characterized in that described host is the mixture of Verticillium lecanii and Imidacloprid, the mass ratio of described Verticillium lecanii and Imidacloprid is 100: 5～50; The preserving number of described verticillium lecanii strain VL-N6 is CCTCC No:M-208086.
2. according to described Verticillium lecanii of claim 1 and the composite desinsection host of Imidacloprid, it is characterized in that described Verticillium lecanii and Imidacloprid mass ratio are 100: 6～25.
3. according to claim 1 or 2 described Verticillium lecaniis and the composite desinsection host of Imidacloprid, it is characterized in that described Verticillium lecanii is Verticillium lecanii bacterium powder or Verticillium lecanii spore suspension.
4. an insecticide that is prepared by claim 1 or 2 described desinsection hosts is characterized in that being made up of host and assistant agent, and host is 5～50% by mass percentage, and assistant agent is 50～95%.
5. according to the described insecticide of claim 4, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 23～30% of the stabilizer comprises compounded pesticides, auxiliary agent accounts for compounded pesticides 7～22%, filler accounts for compounded pesticides 2.5～30%.
6. according to the described insecticide of claim 5, it is characterized in that described stabilizing agent is one or more mixtures in glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, methyl green, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium; Described filler is kaolin, potter's clay or clay.
7. according to claim 4,5 or 6 described insecticides, it is characterized in that described insecticide is wetting powder, suspending agent or finish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100377454A CN101513191B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid |
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| CN2009100377454A CN101513191B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid |
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| CN101513191B true CN101513191B (en) | 2011-09-14 |
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| CN104255808A (en) * | 2014-08-25 | 2015-01-07 | 谢明 | Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control |
| CN106577776A (en) * | 2016-11-25 | 2017-04-26 | 河池市农业科学研究所 | Microbial pesticide for treating corn diseases |
| CN114375962A (en) * | 2021-12-08 | 2022-04-22 | 赤天化科技集团有限公司 | Spirotetramat insecticidal composition and application |
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| 6种常用农药与球孢白僵菌和蜡蚧轮枝菌的相容性;谷祖敏等;《农药》;20060531;第45卷(第5期);325-326 * |
| 谷祖敏等.6种常用农药与球孢白僵菌和蜡蚧轮枝菌的相容性.《农药》.2006,第45卷(第5期),325-326. * |
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