Background technology
Paecilomyces javanicus Isaria javanicus (Friendrichs ﹠amp; Bally) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have excellent characteristics such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Paecilomyces javanicus has important economic implications.Paecilomyces javanicus distributes extensively, red fire ant, prodenia litura, aleyrodid and diamond-back moth etc. is had good control efficiency, has broad application prospects.
Avermectin (Abamec tins) is a kind of never poison, can act on insect synapse or neuromuscular synapse acceptor, the information transmission of nerve endings has activation to chloride channel in the interference insect body, thus the getting in touch of block nerves tip and muscle.Avermectin has paralysis and food refusal effect to insect, and blade is had very strong osmosis, can kill subepidermal insect, and the longevity of residure is long.Avermectin is that a kind of insecticidal spectrum is wide, the speed of knocking down is fast, and the biogenic pesticide of environmentally safe is mainly used in insects such as control diamond-back moth, aphid, Americal rice leaf miner, mite class.
Adopt the agriculture chemical compounding technology or mix the usage amount that application technique can reduce chemical pesticide in the production reality, it is to improve the quick-acting of microorganism insecticide and reduce a residual important method of chemical pesticide that the chemical pesticide of employing sub-doses mixes with biopesticide, the best mixed ratio of screening Paecilomyces javanicus and Avermectin has great importance.The at present domestic correlation technique report that still finds no relevant Paecilomyces javanicus and abamectin compounded insecticide.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Paecilomyces javanicus of noresidue and the compounded pesticides of Avermectin are provided.
Purpose of the present invention realizes by following technical proposals:
The compounded pesticides of a kind of Paecilomyces javanicus and Avermectin is made up of host and assistant agent, and host is 20~60% by mass percentage, and assistant agent is 40~80%; Described host is the mixture of Paecilomyces javanicus and Avermectin, described Paecilomyces javanicus: the Avermectin mass ratio is 100: 0.1~0.5.
Described Paecilomyces javanicus Isaria javanicus (Friendrichs ﹠amp; Bally) bacterial strain IJ-N2; belong to hyphomycetales; paecilomyces; do the nature reserve, hilllock from Guangxi province and separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; this bacterial strain is IJ-N2; the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.In being 200810029158.6 patent application, application number has a detailed description.Paecilomyces javanicus bacterium colony on the Cha Shi medium is stratiform; one deck edge of median rise is irregular; khaki; bacterium colony back side central authorities white; outer rim is faint yellow, and column or ellipse expand in bottle metulae portion, upwards attenuate suddenly; 7.5-12.5 * 2-2.5 μ m, 2~3 wheels are born on the conidiophore.Conidium cylindricality or oblong, 4-6.7 * 1-1.5 μ m is discrete long-chain.
In order to realize the present invention better, the mass ratio of described Paecilomyces javanicus and Avermectin is a Paecilomyces javanicus: Avermectin=100: 0.15~0.45.
As a kind of preferred, described Paecilomyces javanicus is Paecilomyces javanicus conidial powder or Paecilomyces javanicus spore suspension.
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 23~30%, auxiliary agent account for compounded pesticides 7~22%, filler accounts for compounded pesticides 2.5~30%.
Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Paecilomyces javanicus and Avermectin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art; have following advantage and beneficial effect: the bacterial strain that (1) the present invention uses is from being to do the nature reserve, hilllock from Guangxi province to be separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; be China domestic bacterial strain; be not from external introduction, can adapt to domestic natural environment.(2) the present invention has adapted to the requirement that organic food is produced, and environmentally safe, noresidue help delaying the generation of pest resistance to insecticide.(3) the present invention is better than only using a kind of control efficiency of medicament to the control efficiency of insect.Through long-term biological study and the indoor bioassay of infecting, the compounded pesticides of Paecilomyces javanicus and Avermectin has good control efficiency to insects such as prodenia litura, aleyrodid and diamond-back moths, simultaneously mite pest is had certain control effect.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, following embodiment is a preferred implementation of the present invention, but spiritual essence of the present invention and principle are to be based upon great deal of experiment data just to be summed up, simple the substituting of the variation of simple proportioning ratio, similar material can be realized according to this area routine techniques, do not give unnecessary details in an embodiment one by one, but therefore do not limit the present invention.
Following percentage is mass percent, and fungi Imperficti strain is IJ-N2, the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:20% Paecilomyces javanicus Avermectin suspending agent (diluting 2000 times)
A partly is 40% Paecilomyces javanicus conidial suspension: 40% Paecilomyces javanicus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH 7.0, in 4~8 ℃ of preservations.
B partly is a wetting powder: 1.8% Avermectin (commercial) 10%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Paecilomyces varioti Avermectin suspending agent, Paecilomyces javanicus: the Avermectin mass ratio is 100: 0.45.The virulence test result is as shown in table 1.
Embodiment 2:50% Paecilomyces javanicus Avermectin finish (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, 1.8% Avermectin 10% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Paecilomyces varioti Avermectin finish.Wherein, Paecilomyces javanicus bacterium powder: the Avermectin mass ratio is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 3:60% Paecilomyces varioti Avermectin finish (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 60%, 1.8% Avermectin 5% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Paecilomyces javanicus Avermectin finish.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Avermectin is 100: 0.15.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces varioti Avermectin wetting powder (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, 1.8% Avermectin 10% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Paecilomyces javanicus Avermectin wetting powder.Wherein, Paecilomyces javanicus bacterium: the mass ratio of Avermectin is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 5:60% Paecilomyces varioti Avermectin finish (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 60%, 1.8% Avermectin 3% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Paecilomyces javanicus Avermectin finish.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Avermectin is 100: 0.08.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of diamond-back moth (Plutella xylostella)
During Toxicity Determination, (Brassica campestris L.) makes test plant with cabbage mustard, potted plant seedling is put into dependent insect cage, and access diamond-back moth adult, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that diamond-back moth on the cabbage mustard blade grew to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 8 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Paecilomyces javanicus Avermectin is to the Toxicity Determination of diamond-back moth
(10
3Spore/ml, μ g/ml) (behind the medicine 8 days)
Handle |
Virulence regression equation |
R
2 |
LC
50And 95% confidence interval
|
TI |
Paecilomyces javanicus |
y=0.5920x+ 2.1039 |
0.9744 |
7.80,3.04-19.95 |
100.00 |
Avermectin (behind the medicine 2 days) |
y=1.5099x +5.1873 |
0.9849 |
0.75,0.46-1.22 |
|
Example 1: Paecilomyces javanicus: Avermectin (100: 0.45) |
y=0.5306x+ 2.4225 |
0.9542 |
7.21,2.55-20.42 |
108.18 |
Example 2: Paecilomyces javanicus: Avermectin (100: 0.36) |
y=0.5498x+ 2.3946 |
0.9613 |
5.48,1.81-16.70 |
144.44 |
Example 3: Paecilomyces javanicus: Avermectin (100: 0.15) |
y=0.6576x+ 1.9035 |
0.9556 |
5.12,1.89-13.80 |
152.34 |
Example 4: Paecilomyces javanicus: Avermectin (100: 0.36) |
y=0.5267x+ 2.4939 |
0.9840 |
5.73,1.85-17.78 |
136.13 |
Example 5: Paecilomyces javanicus: Avermectin (100: 0.08) |
y=0.5434x+ 2.4081 |
0.9397 |
5.89,1.97-17.38 |
132.43 |
R
2: determination coefficient; TI: relative virus force index.