CN100531575C - Compounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox - Google Patents
Compounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox Download PDFInfo
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- CN100531575C CN100531575C CNB2007100315158A CN200710031515A CN100531575C CN 100531575 C CN100531575 C CN 100531575C CN B2007100315158 A CNB2007100315158 A CN B2007100315158A CN 200710031515 A CN200710031515 A CN 200710031515A CN 100531575 C CN100531575 C CN 100531575C
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- paecilomyces fumosoroseus
- aphox
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Abstract
The invention discloses paecilomyces fumosoroseus and pirimor compounded insecticide. The insecticide is composed of prime solvent and secondary solvent which have weigh percentages that the prime solvent is 10-70 percent and the secondary solvent is 30-90 percent. The prime solvent is the mixture of paecilomyces fumosoroseus and pirimor, and the weight ratio of the paecilomyces fumosoroseus to the pirimor is 100:20-100. The secondary solvent is stabilizing agent, assistant, and filling. The invention discloses paecilomyces fumosoroseus and pirimor compounded insecticide which can be processed into wettable powder, suspending agent, and water disperser. Long-term infectivity biology researches and an indoor biology measurement prove that the paecilomyces fumosoroseus has strong infective and insects killing function on multifold pests, in particular pests with chewing mouthparts. The paecilomyces fumosoroseus and imidacloprid compounded insecticide has strong preventing effect on the pests of whitefly, aphids, and diamondback moth, etc.
Description
Technical field
The present invention relates to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Aphox especially.
Background technology
Paecilomyces fumosoroseus (Paecilomyces fumosoroseus) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use paecilomyces fumosoroseus has important economic implications.Paecilomyces fumosoroseus distributes extensively, aphid, Bemisia tabaci, diamond-back moth etc. is had good control efficiency, has a good application prospect.
Aphox (Pirimicarb) be at present widely used efficient, fumigate, tag, wide spectrum CHE insecticides, suckings pest such as aphid are had special efficacy.Along with using historical prolongation, insect has been developed into a major issue to the pesticide resistance of Aphox.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the Compositional type insecticide of exploitation Aphox is significant.Still find no at present the relevant paecilomyces fumosoroseus and the compounded pesticides of Aphox.
Summary of the invention
In order to overcome the deficiency that above-mentioned prior art exists, the purpose of this invention is to provide a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Aphox.
Purpose of the present invention realizes by following technical proposals: the compounded pesticides of a kind of paecilomyces fumosoroseus and Aphox, form by host and assistant agent, and host is 10～70% by mass percentage, assistant agent is 30～90%; Described host is the mixture of paecilomyces fumosoroseus and Aphox, paecilomyces fumosoroseus: the mass ratio of Aphox is 100: 20～100; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant dinaphthyl methane-disulfonic acid sodium (NNO); Described filler is talcum powder, potter's clay or clay.
Described paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown et Smith belongs to hyphomycetales, and paecilomyces is located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou.Bacterial strain PF01-N10, preserving number: CCTCC No:M on June 27th, 207088,2007 is in China's typical culture collection center preservation.The described Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form verticil mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little pale red, 3～4 * 1～2 μ m, no chlamydospore.
In order to realize the present invention better, described paecilomyces fumosoroseus: the mass ratio of Aphox is 100: 20～50.
As preferably, described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
Described stabilizing agent, auxiliary agent and filler by mass percentage, stabilizer comprises compounded pesticides 5～35%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 3～45%.
The compounded pesticides of described paecilomyces fumosoroseus and Aphox can be processed into formulations such as wetting powder, suspending agent or water dispersant.
The present invention compared with prior art, have following advantage and beneficial effect: the Bemisia tabaci polypide that (1) this bacterial strain is infected from the area, GuangZhou, Guangdong Province separates acquisition, being China domestic bacterial strain, is not from external introduction, can adapt to local natural environment preferably.(2) adapted to the demand that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) its control efficiency is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, paecilomyces fumosoroseus has the very strong insecticidal effect that infects to various pests especially sucking pest.Compounded pesticides of the present invention has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Following percentage is mass percent.The Paecilomyces Fumosoroseus strain is PF01-N10, the bacterial strain preserving number: CCTCC No:M on June 27th, 207088,2007 is in China's typical culture collection center preservation.
Embodiment 1:10% Paecilomyces varioti Aphox suspending agent (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidial suspension: 20% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: Aphox 10%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 10% Paecilomyces varioti Aphox suspending agent, and paecilomyces fumosoroseus: the Aphox mass ratio is 100: 50.The virulence test result is as shown in table 1.
Embodiment 2:5% Paecilomyces varioti Aphox suspending agent (diluting 1000 times)
A partly is 10% paecilomyces fumosoroseus conidial suspension: 10% paecilomyces fumosoroseus conidium is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: Aphox 10%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 5% Paecilomyces varioti Aphox suspending agent, and paecilomyces fumosoroseus: the Aphox mass ratio is 100:100.The virulence test result is as shown in table 1.
Embodiment 3:50% Paecilomyces varioti Aphox wetting powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, Aphox 20%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti Aphox wetting powder.Paecilomyces fumosoroseus: the Aphox mass ratio is 100:40.Virulence test result such as table 1.
Embodiment 4:50% Paecilomyces varioti Aphox wetting powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, Aphox 10%, dodecyl sodium sulfate 14%, Tween-80 10% mixes, and adds talcum powder then and mends to 100%, makes 50% Paecilomyces varioti Aphox wetting powder.Paecilomyces fumosoroseus: the mass ratio of Aphox is 100: 20.
Embodiment 5:10% Paecilomyces varioti Aphox water dispersant (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidium water dispersant: 20% paecilomyces fumosoroseus conidial powder is dissolved in the 20% Tween-80 aqueous solution and the 10% glycerine mixed liquor, and pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: Aphox 10%, and sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 10% Paecilomyces varioti Aphox suspending agent, and paecilomyces fumosoroseus: the Aphox mass ratio is 100:50.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, and potted plant seedling is put into dependent insect cage, and inserts the aphid adult, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI), its result of the test such as table 1 with reference to the abundant method of Sun Yun.
Table 1 Paecilomyces varioti Aphox is to the Toxicity Determination (10 of Bemisia tabaci
5Spore/ml, mg/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | T1 |
| Paecilomyces fumosoroseus | y＝0.3947x+2.6674 | 0.9952 | 8.14，3.56-∞ | 100.00 |
| Aphox (behind the medicine 3 days) | y＝1.5449x+2.8599 | 0.9618 | 23.16，17.91-29.76 | |
| Embodiment 1: paecilomyces fumosoroseus: Aphox (100: 50) | y＝0.3701x+2.8432 | 0.9914 | 6.73，2.87-∞ | 120.95 |
| Embodiment 2: paecilomyces fumosoroseus: Aphox (100: 100) | y＝0.3978x+2.6920 | 0.9912 | 6.33，2.87-∞ | 128.59 |
| Embodiment 3: paecilomyces fumosoroseus: Aphox (100: 40) | y＝0.4227x+2.6470 | 0.9829 | 3.68，1.83-∞ | 221.20 |
| Embodiment 4: paecilomyces fumosoroseus: Aphox (100: 20) | y＝0.3624x+2.9810 | 0.9918 | 3.73，1.68-∞ | 218.23 |
| Embodiment 5: paecilomyces fumosoroseus: Aphox (100: 50) | y＝0.3943x+2.6907 | 0.9927 | 7.20，3.20-∞ | 113.06 |
R
2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1, the compounded pesticides of a kind of paecilomyces fumosoroseus and Aphox is made up of host and assistant agent, it is characterized in that, host is 10～70% by mass percentage, and assistant agent is 30～90%; Described host is the mixture of paecilomyces fumosoroseus (Paecilomyces fumosoroseus) and Aphox, and paecilomyces fumosoroseus: the mass ratio of Aphox is 100: 20～100; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant dinaphthyl methane-disulfonic acid sodium; Described filler is talcum powder, potter's clay or clay; Described Paecilomyces Fumosoroseus strain preserving number: CCTCC No:M207088, on June 27th, 2007 is in China's typical culture collection center preservation.
2, compounded pesticides according to claim 1 is characterized in that, described paecilomyces fumosoroseus: the mass ratio of Aphox is 100:20～50.
3, compounded pesticides according to claim 1 and 2 is characterized in that, described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
4, compounded pesticides according to claim 1 is characterized in that, described stabilizing agent, auxiliary agent and filler by mass percentage, stabilizer comprises compounded pesticides 5～35%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 3～45%.
5, compounded pesticides according to claim 1 and 2 is characterized in that, the compounded pesticides of described paecilomyces fumosoroseus and Aphox is processed into wetting powder, suspending agent or water dispersant.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100315158A CN100531575C (en) | 2007-11-20 | 2007-11-20 | Compounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
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| CNB2007100315158A CN100531575C (en) | 2007-11-20 | 2007-11-20 | Compounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
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| CN100531575C true CN100531575C (en) | 2009-08-26 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101273728B (en) * | 2008-04-22 | 2011-01-19 | 华南农业大学 | Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin |
| CN101273727B (en) * | 2008-04-22 | 2011-01-19 | 华南农业大学 | Pesticides combined with Paecilomyces fumosoroseus and Acetamiprid |
| CN101317581B (en) * | 2008-07-04 | 2011-06-22 | 华南农业大学 | Mixed insecticide of paecilomyces fumosa-roseus brown et smith and avermectin |
| CN101317580B (en) * | 2008-07-04 | 2011-03-23 | 华南农业大学 | Mixed insecticide of paecilomyces fumosa-roseus brown et smith and high-efficiency cypermethrin |
| CN102630710A (en) * | 2012-04-10 | 2012-08-15 | 江苏省农业科学院 | Method for increasing infectivity of Paecilomyces fumosoroseus on Bemisia tabaci by utilizing plant source extract |
Citations (2)
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| KR20000025982A (en) * | 1998-10-16 | 2000-05-06 | 심희진 | Insect pathogenic mold, paecilomyces fumosoroseus sfp-198 strain, microbial insecticides containing it, and method for preventing pine gall midge and vermin using it |
| WO2006121350A1 (en) * | 2005-05-10 | 2006-11-16 | Millennium Microbes Limited | Entomopathogenic fungi and uses thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000025982A (en) * | 1998-10-16 | 2000-05-06 | 심희진 | Insect pathogenic mold, paecilomyces fumosoroseus sfp-198 strain, microbial insecticides containing it, and method for preventing pine gall midge and vermin using it |
| WO2006121350A1 (en) * | 2005-05-10 | 2006-11-16 | Millennium Microbes Limited | Entomopathogenic fungi and uses thereof |
Non-Patent Citations (7)
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| KR100756196B B1 2007.09.05 |
| 常用化学杀虫剂对两种生防真菌孢子的相容性测定. 姜渝，冯明光.应用生态学报，第17卷第7期. 2006 |
| 常用化学杀虫剂对两种生防真菌孢子的相容性测定. 姜渝，冯明光.应用生态学报，第17卷第7期. 2006 * |
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