CN107897214A - A kind of microorganism compounded pesticides for preventing rice grub and its application - Google Patents
A kind of microorganism compounded pesticides for preventing rice grub and its application Download PDFInfo
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- CN107897214A CN107897214A CN201711431517.6A CN201711431517A CN107897214A CN 107897214 A CN107897214 A CN 107897214A CN 201711431517 A CN201711431517 A CN 201711431517A CN 107897214 A CN107897214 A CN 107897214A
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- Prior art keywords
- pymetrozine
- compounded pesticides
- microorganism
- java isaria
- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/707—1,2,3- or 1,2,4-triazines; Hydrogenated 1,2,3- or 1,2,4-triazines
Abstract
The invention discloses a kind of microorganism compounded pesticides for preventing rice grub and its application, the compounded pesticides to contain Java Isaria bacterium and pymetrozine.The present invention provides a kind of compounded pesticides for preventing rice grub, using Java Isaria bacterium and pymetrozine as active ingredient, Java Isaria bacterium has preferable compatibility with pymetrozine, both can significantly improve insecticidal effect at compounding, effectively improves the resistance to the action of a drug of the pest to pymetrozine.The dosage of pesticide pymetrozine can be reduced using compound insecticidal agent to preventing and controlling pest, the pesticide residue on crop can be reduced, it is environmentally safe, have a vast market prospect.
Description
Technical field
The present invention relates to biological pesticide technical field, is compounded more particularly, to a kind of microorganism for preventing rice grub
Insecticide and its application.
Background technology
Planthopper includes brown paddy plant hopper, white backed planthopper etc., is the important pests on rice, widely distributed in China, in south
Rice producing region brown paddy plant hopper, white backed planthopper rice can be caused to drop in production over a large area when pest seriously occurs.It is in prevention to use more
Traditional chemical pesticide such as Diacloden, imidacloprid, pymetrozine is prevented, and causes brown fly using chemical insecticide in large quantities for a long time
The rice grubs such as lice, white backed planthopper generate the different degrees of resistance to the action of a drug, while chemical pesticide kills natural enemy, destroys rice field
Ecological environment, weaken the capacity of self-regulation of Ecological Environment of Paddy Field.Therefore, safe efficient, sustainable control rice is researched and developed to fly
The microbial pesticide of lice, which substitutes chemical pesticide, has important economic results in society and ecological benefits.
Pymetrozine also known as pyrazinones, belong to pyridines or Triazinone insecticide, main for brand-new non-killing livestock property insecticide
The lancet of sucking pest is acted on, causes the lancet of pest to be blocked and stop feeding, and it is final hungry and dead, because
It is and notable to the prevention effect of sucking pest.Pymetrozine has pest action of contace poison, while also systemic activity, energy
Xylem and phloem transport in plant.Suitable for it is anti-eliminate aphis, aleyrodid, the homoptera pest such as leafhopper and plant hopper.Closely
The wide, extensive use of the pesticide on rice such as pymetrozine, causes the resistance to the action of a drug of the rice grubs such as planthopper to be remarkably reinforced over year.
Therefore, how to reduce the dosage of insecticide, slow down pest pesticide it is drug-fast growth have become it is urgently to be resolved hurrily
Problem, exploitation material compounded pesticides are of great significance.
The usage amount of pesticide in produce reality can be reduced using pesticide combination technology or mixing application technique, slows down pest
To existing chemical pesticide resistance to the action of a drug growth rate, it is to improve microbial insecticide to be mixed using the pesticide of sub-doses with biological pesticide
Quick-acting and reduce chemical usage amount, the remaining important method of chemical pesticide.
The content of the invention
The purpose of the present invention is intended to overcome above-mentioned the deficiencies in the prior art, there is provided a kind of Java Isaria bacterium and pymetrozine
Compounded pesticides, the usage amount of chemical pesticide in produce reality is reduced using pesticide combination technology or mixing application technique, is slowed down
Pest is to existing chemical pesticide resistance to the action of a drug growth rate.
Application another object of the present invention is to the compounded pesticides for providing mentioned microorganism fungi and pymetrozine.
The above-mentioned purpose of the present invention is achieved by following technical solution:
A kind of microorganism compounded pesticides for preventing rice grub, contain Java Isaria bacterium and pymetrozine.
Preferably, the mass ratio of the Java Isaria bacterium and pymetrozine is 100:8~35.
It is highly preferred that the mass ratio of the Java Isaria bacterium and pymetrozine is 100:8.33~33.3.
It is highly preferred that the mass ratio of the Java Isaria bacterium and pymetrozine is 100:20.
Java Isaria bacterium is a kind of insect pathogenic fungus, the prevention effect of sucking pest is protruded, as one
Kind of living body biological pesticide, has the brand-new mechanism of action different from existing chemical insecticide, environmentally safe, noresidue
Feature.And Java Isaria bacterium has the preferable resistance to the action of a drug to pymetrozine, both compatibilities are good, using sub-doses pesticide with
Quick-acting, the usage amount for reducing chemical pesticide and the chemical pesticide that Java Isaria bacterium mixing can improve microbial insecticide are residual
Stay, insect pathogenic fungus and insecticide are carried out science mixture can not only lift insecticidal effect, be imitated relative to the prevention of single dose
Fruit has greatly improved, and largely slow down the resistance to the action of a drug of the pest to chemical insecticide.
Preferably, the Java Isaria bacterium is Java Isaria IJNL-N8, and the bacterial strain was in preservation on November 22 in 2017
In China typical culture collection center, deposit number is CCTCC NO: M 2017709.
This bacterial strain is separated from the brown paddy plant hopper polypide that Guangdong Province In Guangzhou Area is infected and obtained, and is China domestic bacterial strain,
Not introduced from foreign countries, adapt to local natural environment.
Java Isaria IJNL-N8 bacterium colony shape in czapek's medium is irregular, yellow-white in the middle part of the bacterium colony back side, to
It is changed into Chu Shise outside;Mycelia is thin, there is fine-grained structure on surface, white, and mycelia is 0.3 ~ 1.2 μm wide, separates, transparent, smooth;It is mitogenetic
9~13 μm of sporophore, thereon the verticil of raw 2~4 bottles stalk composition, bottle metulae portion ellipse expand, be tapered upwards,
8 ~ 14 × 2.2~2.8 μm;The nearly cylindricality of conidium or fusiformis, transparent, smooth, 5.0~8 × 1.4~3 μm, conidium is concatenated
It is arranged in long-chain.
Preferably, the Java Isaria IJNL-N8 bacterium are Java Isaria IJNL-N8 conidial powders.
The compounded pesticides are made of host agent and assistant agent, and the host agent is the mixing of Java Isaria bacterium and pymetrozine
Thing, the assistant agent are stabilizer, auxiliary agent and filler, and each component is by mass percentage:Host agent 35~65%, stabilizer 0.5~
1%, auxiliary agent 11~20%, filler 14~58.5%.
Preferably, the stabilizer is nano-aluminum hydroxide, glycerine or paraffin oil;The auxiliary agent is Tween-80, lignin
Sodium sulfonate, wetting agent LX, up to profit suspending agent DY6 in one or more;The filler is kaolin, diatomite, clay, hard charcoal
Black or methyl oleate.
Preferably, compounded pesticides are wettable powder or finish.
A kind of application of mentioned microorganism compounded pesticides on prevention rice grub.
Bacterial strain Java Isaria IJNL-N8 is to separate to obtain on the planthopper from Guangdong Province In Guangzhou Area by natural infection
A kind of insect pathogenic fungus, can effectively prevent planthopper in southern area;The bacterium has pymetrozine the stronger resistance to the action of a drug, with
The pymetrozine of low concentration, which is used in mixed way, can thoroughly control planthopper, can greatly reduce the usage amount of the chemical pesticides such as pymetrozine, solution
The certainly resistance problems and Pesticide Residue during water rice hopper prevention.
Compared with prior art, the present invention has the advantages that:
The present invention provides a kind of microorganism compounded pesticides for preventing rice grub, using Java Isaria bacterium and pymetrozine to have
Component is imitated, Java Isaria bacterium has good compatibility with pymetrozine, both compound the desinsection that can be significantly improved to planthopper
Effect, is effectively improved the resistance to the action of a drug of the pest to pymetrozine.Reduce chemical pesticide pyrrole aphid using microorganism compounded pesticides at the same time
The dosage of ketone, reduces the residual of chemical pesticide, environmentally safe, has a vast market prospect.
Embodiment
With reference to embodiment, the present invention is further illustrated, but embodiment the present invention is not done it is any
The restriction of form.Unless otherwise indicated, the source chemicals that the embodiment of the present invention uses is the source chemicals routinely bought.
The acquisition of 1 Java Isaria IJNL-N8 bacterial strains of embodiment
Inventor team is collected on the rice of In Guangzhou Area by brown paddy plant hopper [the Nilaparvata lugens of worm fungal infection
(Stal)], separate, purify from worm corpse, screening, identification, obtaining one plant of Java cluster to planthopper with fine preventive effect
Spore IJNL-N8, and the bacterial strain has the resistance to the action of a drug to pymetrozine.The bacterial strain is preserved in Chinese Typical Representative on November 22nd, 2017
Culture collection, deposit number are CCTCC NO: M 2017709.
2 Java Isaria IJNL-N8 bacterial strains of embodiment measure the resistance to the action of a drug of pymetrozine
1st, the processing of strains tested
The Java Isaria IJNL-N8 inoculations obtained after purified are on czapek's medium tablet, in 25 ± 1 DEG C of perseverance
Incubator(14:10D)After middle culture 10 days, collect spore with the sterile water of various concentrations pymetrozine and suspension is made, use glass slide
Sprouting method is tested.Spore suspension is dropped on sterilized slide glass, bottom is placed in and is covered with the culture dish of filter paper, 3 are added dropwise in ware
~4 drop sterile water moisturizings(100, RH), microscopy after 11h, 13h, 15h is cultivated, calculates spore germination rate(Spore germination rate %=
The % of the spore count of sprouting/total spore count × 100).Each 3 repetitions of processing.Above-mentioned all test datas are soft in data processing
Handle and complete on part SAS system.
2nd, result of study shows, the pymetrozine of 0.5 and 0.1 times of conventional dosage is on the spore germination rate of bacterial strain without influence
(Table 1), the spore germination rate of Java Isaria IJNL-N8 bacterial strains is up to more than 90%;In the pyrrole of 11h and 13h routine dosages
Aphid ketone can significantly reduce the spore germination rate of bacterial strain, but after 15h various concentrations pymetrozine to the spore germination rate of bacterial strain
Influence difference is not notable, and the spore germination rate of Java Isaria IJNL-N8 bacterial strains is up to more than 93%.
Spore germination rate of the 1 Java Isaria IJNL-N8 bacterial strains of table in various concentrations pymetrozine solution
Time | Check plot | Pymetrozine (1x) | Pymetrozine (0.5x) | Pymetrozine (0.1x) |
11h | 91.4±4.7 a | 79.3±4.5 b | 89.2±4.1 a | 90.7±4.1 a |
13h | 96.8±7.1 a | 89.5±4.1 b | 92.3±3.8 a | 95.9±535 a |
15h | 97.6±5.3 a | 93.1±5.8 a | 95.3±6.1 a | 96.5±4.3 a |
Note:The identical person of letter represents that difference is not notable after numeral of going together in table(DMRT methods).Pymetrozine (1x, 0.5x and 0.1x) point
Not Biao Shi pymetrozine dosage for routine dose, 0.5 and 0.1 times of routine dose.
3 30% Java Isaria IJNL-N8 compounded pesticides wettable powders of embodiment(2000 times of dilution)
30% Java Isaria IJNL-N8 bacterium powders(1010Spore/g), 10% pymetrozine(Active compound purchased in market), 0.8% nanometer of hydroxide
Aluminium, 5% Tween-80,6% sodium lignin sulfonate, 5% up to profit suspending agent DY6, and diatomite is mended to 100%, and 30% Java Isaria is made
IJNL-N8 pymetrozine wettable powders, wherein Java Isaria IJNL-N8 bacterium powders:The mass ratio of pymetrozine is 100:33.3.
4 60% Java Isaria IJNL-N8 compounded pesticides wettable powders of embodiment(2000 times of dilution)
60% Java Isaria IJNL-N8 bacterium powders(1010Spore/g), 5% pymetrozine(Active compound purchased in market), 0.8% nano-aluminum hydroxide,
5% Tween-80,6% sodium lignin sulfonate, kaolin are mended to 100%, and 60% Java Isaria IJNL-N8 pymetrozine wettables are made
Pulvis, wherein Java Isaria IJNL-N8 bacterium powders:The mass ratio of pymetrozine is 100:8.33.
5 50% Java Isaria IJNL-N8 compounded pesticides wettable powders of embodiment(2000 times of dilution)
50% Java Isaria IJNL-N8 bacterium powders(1010Spore/g), 10% pymetrozine(Active compound purchased in market), 1% glycerine, 15% tween-
80, diatomite is mended to 100%, and 50% Java Isaria IJNL-N8 pymetrozine wettable powders, wherein Java Isaria is made
The mass ratio of IJNL-N8 Jun Fen ﹕ pymetrozines is 100:20.
6 50% Java Isaria IJNL-N8 compounded pesticides wettable powders of embodiment(2000 times of dilution)
50% Java Isaria IJNL-N8 bacterium powders(1010Spore/g), 5% pymetrozine(Active compound purchased in market), 1% glycerine, 15% tween-
80, diatomite is mended to 100%, and diatomite is mended to 100%, and 50% Java Isaria IJNL-N8 pymetrozine wettable powders are made, its
In, Java Isaria IJNL-N8 bacterium powders:The mass ratio of pymetrozine is 100:10.
7 50% Java Isaria IJNL-N8 compounded pesticides finishes of embodiment(2000 times of dilution)
50% Java Isaria IJNL-N8 bacterium powders(1010Spore/g), 10% pymetrozine(Active compound purchased in market), 1% glycerine, 15% tween-
80, add methyl oleate to mend to 100%, 50% Java Isaria IJNL-N8 pymetrozine finishes are made, wherein, Java Isaria IJNL-
N8 Jun Fen ﹕ pymetrozines mass ratio is 100:20.
Toxicity test of 8 compounded pesticides of embodiment to brown paddy plant hopper [Nilaparvata lugens (Stal)]
1st, experimental method
(1)Test object
With rice (Oryza sativaL. test plant) is made, by Potted orchard(Basin diameter 30cm, height of seedling 40cm, 3 clumps/basin, 6 ~
13 plants/clump)It is put into insect cage, and accesses brown paddy plant hopper adult, drives its whole out of after it is laid eggs one day, the interior holding 26 of cage ±
1 DEG C of temperature and 14:10 illumination, treats that the brown paddy plant hopper of rice stem is developed to 3,4 age nymphs.
(2)Experiment packet
Tested using the compounded pesticides of embodiment 3~7, while be provided with following control group:
Contrast groups 1:A kind of compounded pesticides, it is identical with 3 compounded pesticides basic components constituent content of embodiment, but do not include pawl
Isaria IJNL-N8 bacterium conidia powder and pymetrozine.
Contrast groups 2:A kind of compounded pesticides, it is identical with 3 compounded pesticides basic components constituent content of embodiment, distinguish
In 30% Java Isaria IJNL-N8 bacterium powders(1010Spore/g)40% Java Isaria IJNL-N8 bacterium are changed to 10% pymetrozine
(106Spore/ml)Single dose, dilutes 2000 times.
Contrast groups 3:A kind of compounded pesticides, it is identical with 3 compounded pesticides basic components constituent content of embodiment, distinguish
In 30% Java Isaria IJNL-N8 bacterium powders(1010Spore/g)It is 40% pymetrozine with 10% pymetrozine(50µg/ml)Single dose, dilution
2000 times.
(3)Test method
Liquid is sprayed onto on rice leaf and stem using spray-on process, be stained with rice liquid droplet without dripping preferably, each
6 basin seedlings are handled, investigate the effect of 10 days after medicine, calculate corrected mortality.
2nd, test result
Entirely 4 repetitions of experiment, corrected mortality, corrected mortality are calculated according to equation below(%)=(Handle the death rate-control
The death rate)/(1- compares the death rate)×100.
Its result of the test is as shown in table 2.
Prevention effect of 2 different agents of table to planthopper
Processing | Insect population before medicine(Only) | Population number after medicine(Only) | Prevention effect(%) |
Embodiment 3 | 2945.8±296.0 | 317±65.7 | 89.2±4.9 |
Embodiment 4 | 2913.3±373.1 | 519±72.0 | 82.2±3.5 |
Embodiment 5 | 3183.5±325.1 | 210.5±65.8 | 93.4±5.7 |
Embodiment 6 | 3029±315.9 | 602.5±84.8 | 80.1±5.1 |
Embodiment 7 | 2717.3±316.7 | 231.75±44.7 | 91.5±4.4 |
Contrast groups 1 | 2819.5±265.4 | 2473±266.0 | 12.2±3.2 |
Contrast groups 2 | 3015.5±291.1 | 1136.5±129.8 | 62.3±5.7 |
Contrast groups 3 | 3057±295.7 | 1559.8±204.4 | 48.9±4.2 |
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from the limit of above-described embodiment
System, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of microorganism compounded pesticides for preventing rice grub, it is characterised in that contain Java Isaria bacterium and pymetrozine.
2. microorganism compounded pesticides according to claim 1, it is characterised in that the Java Isaria bacterium and pymetrozine
Mass ratio is 100:8~35.
3. microorganism compounded pesticides according to claim 2, it is characterised in that the Java Isaria bacterium and pymetrozine
Mass ratio is 100:8.33~33.3.
4. microorganism compounded pesticides according to claim 2, it is characterised in that the Java Isaria bacterium and pymetrozine
Mass ratio is 100:20.
5. according to any microorganism compounded pesticides of Claims 1 to 4, it is characterised in that the Java Isaria bacterium is
Java Isaria IJNL-N8, the bacterial strain were preserved in China typical culture collection center, deposit number on November 22nd, 2017
For CCTCC NO: M 2017709.
6. microorganism compounded pesticides according to claim 5, it is characterised in that the Java Isaria IJNL-N8 is pawl
Isaria IJNL-N8 conidial powders.
7. according to any microorganism compounded pesticides of Claims 1 to 4, it is characterised in that the compounded pesticides are by leading
Agent and assistant agent composition, the host agent are Java Isaria bacterium and the mixture of pymetrozine, and the assistant agent is stabilizer, auxiliary agent and fills out
Material, each component are by mass percentage:Host agent 35~65%, stabilizer 0.5~1%, auxiliary agent 11~20%, filler 14~
58.5%。
8. microorganism compounded pesticides according to claim 7, it is characterised in that the stabilizer for nano-aluminum hydroxide,
Glycerine or paraffin oil;The auxiliary agent for Tween-80, sodium lignin sulfonate, wetting agent LX, up to profit suspending agent DY6 in one kind or
It is several;The filler is kaolin, diatomite, clay, white carbon or methyl oleate.
9. microorganism compounded pesticides according to claim 7, it is characterised in that the compounded pesticides are wettable powder
Or finish.
10. application of any one of Claims 1 to 4 microorganism compounded pesticides on prevention rice grub.
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Cited By (3)
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CN110720471A (en) * | 2019-11-15 | 2020-01-24 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutagenic strain IJUV-6 and thiamethoxam and application thereof |
CN110839652A (en) * | 2019-11-15 | 2020-02-28 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof |
CN112715301A (en) * | 2021-01-22 | 2021-04-30 | 江苏省农业科学院 | Method for promoting rice growth and repelling pests by utilizing Isaria javanicus |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110720471A (en) * | 2019-11-15 | 2020-01-24 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutagenic strain IJUV-6 and thiamethoxam and application thereof |
CN110839652A (en) * | 2019-11-15 | 2020-02-28 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof |
CN110839652B (en) * | 2019-11-15 | 2021-07-27 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof |
CN110720471B (en) * | 2019-11-15 | 2021-07-27 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutagenic strain IJUV-6 and thiamethoxam and application thereof |
CN112715301A (en) * | 2021-01-22 | 2021-04-30 | 江苏省农业科学院 | Method for promoting rice growth and repelling pests by utilizing Isaria javanicus |
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