CN110839652B - Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof - Google Patents
Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof Download PDFInfo
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Abstract
The invention discloses a compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof. The invention firstly provides a compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran, wherein the compound pesticide contains Isaria javanicus mutant strain IJUV-6 and dinotefuran. The isaria javanica mutant strain IJUV-6 has good compatibility with dinotefuran, the dinotefuran does not influence the germination and hypha growth of the isaria javanica mutant strain IJUV-6 spores, and the combination of the low-dose dinotefuran and the isaria javanica mutant strain IJUV-6 can play a role in synergy, so that the control effect on rice planthoppers and rice leafhoppers is obviously improved, the use amount of chemical pesticide dinotefuran can be reduced, and the drug resistance of the rice planthoppers and rice leafhoppers to the dinotefuran is improved; in addition, the compound pesticide has stable prevention and control effect on rice planthoppers and rice leafhoppers under the conditions of strong ultraviolet rays and high temperature, has no pollution and residue, and has wide application prospect in prevention and control of the rice planthoppers and the rice leafhoppers.
Description
Technical Field
The invention belongs to the technical field of biological control. More particularly relates to a compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof.
Background
Rice planthopper and rice leafhopper are migratory insects on rice. The rice planthopper flies back and forth in the rice planting areas in the south and the middle of China along with airflow in spring and autumn every year, the rice planthopper not only directly stabs and absorbs rice juice and the like, but also more seriously spreads rice virus diseases, and dwarf diseases caused in the tillering stage and the jointing stage of rice can cause that the yield of the rice is reduced by more than 50 percent in a large area, and even the rice is harvested absolutely. In the aspect of prevention and treatment, chemical pesticides such as dinotefuran, thiamethoxam, imidacloprid, pymetrozine and the like are mostly adopted for prevention and treatment; however, the long-term and large-scale use of chemical pesticides causes the rice planthoppers and rice leafhoppers to generate drug resistance, and meanwhile, the large-scale use of chemical pesticides kills natural enemies of pests, destroys the self-regulation capability of the rice field ecosystem on the pests, and pollutes water and ecological environment.
Isaria javanicanicus is an important entomopathogenic fungus, has outstanding control effect on pests with piercing-sucking mouthparts such as rice planthoppers and rice leafhoppers, has a brand new action mechanism different from the existing chemical insecticides as a living microbial pesticide, can be used for treating drug-resistant pests, and has the characteristics of no pollution to the environment, no residue, being friendly to natural enemies of the pests and the like; however, field application practices show that the control effect of the live microbial insecticides such as Isaria javanicus is easily influenced by environmental conditions such as strong ultraviolet rays and high temperature in the field. Therefore, the research and development of the microbial pesticide for continuously controlling the rice pests, which is environment-friendly, safe and efficient, has important social and economic benefits and ecological benefits instead of chemical pesticides.
Dinotefuran is a novel super-nicotine pesticide, can be absorbed by the roots of plants and widely distributed in the plants, has obvious control effect on piercing-sucking mouthpart pests, can show high insecticidal activity at a lower dose, has the characteristics of contact poisoning, stomach toxicity, strong systemic property, quick effect, long lasting period and the like, and is suitable for controlling aphids, leafhoppers, plant hoppers, thrips, whiteflies and resistant pests thereof. However, in recent years, the pesticide resistance of rice pests such as rice planthoppers and rice leafhoppers is remarkably enhanced due to the wide and large use of pesticides such as dinotefuran in rice.
Therefore, on the premise of effectively controlling rice pests and improving rice yield, the using amount or using times of chemical pesticides is reduced, the increase of the drug resistance of the pests to the chemical pesticides is slowed down, the ecological environment of a farmland is protected, the main problem to be solved urgently in current rice production is solved, and the development of the efficient microbial compound pesticide which is environment-friendly and safe to natural enemies of the pests is of great significance.
Disclosure of Invention
The invention aims to overcome the defects of the existing rice pest control technology and provides a compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof.
The invention aims to provide a compound insecticide.
The invention also aims to provide application of the compound pesticide in controlling rice planthoppers/rice leafhoppers or preparing a biological preparation for controlling the rice planthoppers/the rice leafhoppers.
The invention also aims to provide a biological agent for preventing and controlling rice planthopper/rice leafhopper.
The above purpose of the invention is realized by the following technical scheme:
the invention firstly provides a compound insecticide which contains Isaria javanicus and dinotefuran.
Preferably, the mass ratio of the Isaria javanicus to the dinotefuran is 100:8.33 to 25.
More preferably, the mass ratio of the Isaria javanicus to the dinotefuran is 100:10 to 16.
Still more preferably, the mass ratio of the Isaria javanicus to the dinotefuran is 100: 16.
preferably, the Isaria javanicus is Isaria javanicus (Isaria japonica) mutant strain IJUV-6 which is preserved in China center for type culture collection 10 months and 10 days in 2019 and has the preservation number of CCTCC NO: m2019800, the preservation address is Wuhan, Wuhan university in China.
Preferably, the Isaria javanicus mutant strain IJUV-6 is spore powder of the Isaria javanicus mutant strain IJUV-6.
The Isaria javanicus mutant strain IJUV-6 provided by the invention has good biocompatibility with low-dose dinotefuran, the dinotefuran does not influence the germination and hypha growth of Isaria javanicus mutant strain IJUV-6 spores, and the compound insecticide prepared by compounding the low-dose dinotefuran and the Isaria javanicus mutant strain IJUV-6 has obvious control effects on rice planthoppers and rice leafhoppers; therefore, the application of the compound pesticide in controlling the rice planthopper/rice leafhopper or preparing the biological preparation for controlling the rice planthopper/rice leafhopper also belongs to the protection scope of the invention.
The invention also provides a biological agent for preventing and treating rice planthopper/rice leafhopper, which consists of a main agent and an auxiliary agent; the main agent comprises Isaria javanicus mutagenic strain IJUV-6 and dinotefuran, and the auxiliary agent comprises a stabilizer, an auxiliary agent and a filler.
Preferably, the biological agent comprises, in mass percent: 50-65% of main agent, 0.3-0.8% of stabilizing agent, 12-16% of auxiliary agent and 22-35% of filler.
More preferably, the biological agent comprises, in mass percent: 58% of main agent, 0.8% of stabilizing agent, 14% of auxiliary agent and 27.2% of filler.
Preferably, the stabilizer is nano aluminum hydroxide or glycerol.
Preferably, the auxiliary agent is any one or more of tween-80, sodium lignosulfonate, Daruning suspending agent DK8, span-20, Morwet D-425, DP-10 or sodium carboxymethylcellulose.
Preferably, the filler is any one or more of diatomite, kaolin, white carbon black or paraffin oil.
Preferably, the biological preparation is in the form of wettable powder or oil.
Preferably, the rice planthopper is sogatella furcifera.
The invention has the following beneficial effects:
the invention provides a compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof. The compound pesticide takes ultraviolet-resistant and high-temperature-resistant Isaria javanicus mutant strain IJUV-6 and dinotefuran as effective components, the Isaria javanicus mutant strain IJUV-6 and dinotefuran have good compatibility, the dinotefuran does not influence the germination and hypha growth of Isaria javanicus mutant strain IJUV-6 spores, the compound of the low-dose dinotefuran and Isaria javanicus mutant strain IJUV-6 can play a role in synergy, and the control effects on rice planthoppers and rice leafhoppers are obviously improved; the compound pesticide is applied to control rice planthoppers and rice leafhoppers, the using amount and the using frequency of the chemical pesticide dinotefuran can be reduced, the increase speed of the pesticide resistance of pests to the dinotefuran is effectively slowed down, and the problem of pesticide resistance and the problem of pesticide residue in the process of controlling the rice planthoppers and the rice leafhoppers are solved;
in addition, compared with the isaria javanica single agent, the compound pesticide has the characteristic of quick-acting performance, has stable control effect on rice planthoppers and rice leafhoppers, is not influenced by field environmental conditions such as strong ultraviolet rays, high temperature and the like in southern areas, has no pollution to the environment, is beneficial to protecting natural enemies of pests, continuously controlling harm of the rice planthoppers and the rice leafhoppers, promotes the improvement of the yield and the quality of rice, and has wide market application prospect.
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FIG. 1 is a graph showing the results of ultraviolet ray resistance tests of Isaria javanicus mutant strain IJUV-6.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 acquisition of Isaria javanicus mutant strain IJUV-6
Brown plant hoppers (Nilapavata lugens (Stal)) infected by insect fungi are collected on rice in Guangzhou area by an inventor team, and a Isaria javanicus IJNL-N8 (preservation number: CCTCC NO: M2017709) with good control effect on rice plant hoppers and rice leafhoppers is obtained after separation and screening from dead bodies. In later-period field application, the strain is found to have unstable control effect on rice planthoppers, rice leafhoppers and other rice pests, and is easily influenced by environmental conditions such as strong ultraviolet rays, high temperature and the like in fields. Therefore, the wild strain (Isaria javanicus IJNL-N8) is irradiated by ultraviolet rays to induce the strain to generate variation, and the Isaria javanicus mutagenic strain IJUV-6 with ultraviolet ray resistance and high temperature resistance is finally obtained through screening and purification and is preserved in the China center for type culture collection 10 months and 10 days in 2019 with the preservation number of CCTCC NO: m2019800, the preservation address is Wuhan, Wuhan university in China.
Example 2 determination of compatibility of Isaria javanicus mutant IJUV-6 with dinotefuran
1. Experimental methods
Ultraviolet-resistant Isaria javanicus mutant strain IJUV-6 is inoculated into a Chaudhur culture medium (30.0 g of sucrose and sodium nitrate (NaNO)3)3.0g of dipotassium hydrogen phosphate (K)2HPO4)1.0g magnesium sulfate (MgSO)4.7H20.5g of O), 0.5g of potassium chloride (KCl), and ferrous sulfate (FeSO)4)0.01g and 20g of agar powder, adding water to make the total volume be 1000mL), culturing in a thermostat (L: D is 14:10) at 26 +/-1 ℃ for 10 days, collecting spores by using 0.1% Tween-80 sterile water, mixing fungal spores and dinotefuran in a Chache culture solution to prepare suspensions containing different concentrations of dinotefuran (0.1x, 0.5x and 1x) and fungal spores, and testing by using a slide germination method;
dropping the dinotefuran and spore suspension with different concentrations on a sterile glass slide, placing the sterile glass slide on a culture dish with filter paper laid at the bottom, dropping sterile water for moisturizing (100, RH), culturing for 9h, 11h and 13h, and performing microscopic examination to calculate the spore germination rate (the spore germination rate is the number of germinated spores/total spore number multiplied by 100%). Each treatment was repeated 3 times. All the test data are processed and completed on a data processing software SAS system.
2. Results of the experiment
The result of spore germination rates of the Isaria javanicus mutant strain IJUV-6 in dinotefuran solutions with different concentrations is shown in Table 1, and it can be seen that, compared with a control group, dinotefuran (0.1x, 0.5x, 1x) with different concentrations has no significant difference on the spore germination rate of the Isaria javanicus mutant strain IJUV-6, the spore germination rates of the Isaria javanicus mutant strain IJUV-6 are not influenced by the length of the culture time (9 h, 11h and 13h of culture), and the spore germination rates can reach over 86.5%. The above results indicate that the Isaria javanicus mutant strain IJUV-6 has good compatibility with dinotefuran.
TABLE 1 spore germination rates of Isaria javanicus mutant strain IJUV-6 in dinotefuran solutions of different concentrations
| Time | Control group | Dinotefuran (1x) | Dinotefuran (0.5x) | Dinotefuran (0.1x) |
| 9h | 87.1±3.4a | 86.5±2.9a | 87.9±4.5a | 88.3±3.6a |
| 11h | 95.5±3.7a | 94.8±3.2a | 95.8±2.8a | 96.1±4.2a |
| 13h | 98.4±2.9a | 97.7±3.5a | 98.6±3.3a | 99.1±3.4a |
Note: the same letters after the same number in the same column in the table indicate that the difference is not significant (DMRT method); dinotefuran (1x), dinotefuran (0.5x) and dinotefuran (0.1x) respectively represent the use dose of dinotefuran as the field conventional use dose and the field conventional use dose of 0.5 and 0.1 times.
Example 3 high temperature and ultraviolet resistance test of Isaria javanicus mutant strain IJUV-6
1. Experimental methods
Respectively inoculating conidia of a wild strain (WT) and a mutant strain (UV) into a PDA (personal digital assistant) plate to be cultured until conidia are produced for later use, respectively collecting conidia of the two strains by using 0.1% Tween-80 sterile water to prepare 1.0 multiplied by 106 spores/mL of spore suspension, placing the spore suspension under ultraviolet radiation for 0 to 40min, then adding 5mL of the spore suspension into a triangular flask filled with 200mL of Chachi's culture solution, culturing at 26 +/-1 ℃ and 200rpm, observing and recording the germination conditions at 10h, 12h and 14h, taking the condition that the length of a germ tube exceeds half of the diameter of the spores as a germination standard, and repeating all the treatments for 3 times; the ultraviolet resistance of the Isaria javanicus mutant strain IJUV-6 is determined by comparing the difference of the strains in the 10h, 12h and 14h in the spore germination rate.
In addition, spore suspension with the concentration of 1.0 × 106 spores/mL is taken and cultured under the conditions of 30 +/-1 ℃, 31 +/-1 ℃, 31.5 +/-1 ℃, 33.5 +/-1 ℃, 34.5 +/-1 ℃, 35 +/-1 ℃ and 200rpm respectively, the spore germination conditions are observed and recorded at 12h and 24h, the germination standard is that the length of a germ tube exceeds half of the diameter of the spores, and all treatments are repeated for 3 times; the high temperature resistance of the Isaria javanicus mutant strain IJUV-6 is determined by comparing the difference of spore germination rates of the strains at different temperatures in 12h and 24 h.
2. Results of the experiment
The ultraviolet resistance test results of the Isaria javanicus mutant strain IJUV-6 are shown in figure 1, and it can be seen that after the Isaria javanicus mutant strain IJUV-6 is irradiated by ultraviolet rays for 40min, the spore germination rate of the Isaria javanicus mutant strain IJUV-6 is not obviously different from that of a control group (irradiated by ultraviolet rays for 0min), while after the wild strain is irradiated by ultraviolet rays for 40min, the spore germination rate of the wild strain is obviously different from that of the control group.
The above results illustrate that: the ultraviolet irradiation has no obvious influence on the spore germination rate of the Isaria javanicus mutant strain IJUV-6, and the Isaria javanicus mutant strain IJUV-6 has good ultraviolet resistance.
The results of the spore germination rates of the Isaria javanicus mutant strain IJUV-6 at different temperatures are shown in Table 2, and it can be seen that spores of both the wild strain and the mutant strain IJUV-6 can normally germinate at the temperature of 30 +/-1 ℃ to 33.5 +/-1 ℃; wherein the spore germination rate of the mutagenic strain IJUV-6 at 33.5 +/-1 ℃ is obviously higher than that of a wild strain; the spore germination rate of the mutagenized strain IJUV-6 at 24h is more than 20 percent under the condition that the temperature is 34.5 +/-1 ℃, and the wild strain does not germinate; and spores of Isaria javanicus IJN-30 and Isaria javanicus IJW-25 do not germinate under the condition that the temperature is higher than 33.5 +/-1 ℃.
The above results show that: the mutant strain IJUV-6 obtained by screening has good high temperature resistance.
TABLE 2 spore germination (%) results of the mutagenized strain IJUV-6 at different temperatures
Note: the same letters after the same numbers in the same column indicate no significant difference (DMRT method).
Example 450% wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran
A wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran comprises the following specific formula components: 50% Isaria javanicus mutant strain IJUV-6 spore powder (10)11Spore/g), 8% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 4% tween-80, 5% sodium lignosulfonate, 5% damantyl suspending agent DK8, and diatomite for supplementing to 100% to prepare 50% wettable powder of the isaria javanica mutant strain IJUV-6 and dinotefuran; wherein the mass ratio of the Isaria javanicus mutant strain IJUV-6 to dinotefuran is 100: 16.
Example 550% wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran
A wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran comprises the following specific formula components: 50% Isaria javanicus mutant strain IJUV-6 spore powder (10)11Spore/g), 5% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 4% span-20, 5% Morwet D-425, 4% sodium carboxymethylcellulose and kaolin to make up to 100%, so as to prepare 50% wettable powder of Isaria javanicus mutant IJUV-6 and dinotefuran; wherein the mass ratio of the Isaria javanicus mutant strain IJUV-6 to dinotefuran is 100: 10.
Example 640% wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran
A wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran comprises the following specific formula components: 40% Isaria javanicus mutant strain IJUV-6 spore powder (10%11Spore/g), 10% dinotefuran (technical product purchased from market), 0.3% nano-aluminium hydroxide, 3% tween-80, 6% sodium lignosulfonate, 6% sodium carboxymethylcellulose and diatomite which are supplemented to 100% to prepare 40% wettable powder of the Isaria javanicus mutant strain IJUV-6 and dinotefuran; wherein the mass ratio of the Isaria javanicus mutant strain IJUV-6 to dinotefuran is 100: 25.
Example 760% wettable powder of Isaria javanicus mutant strain IJUV-6 and dinotefuran
An oil solution of Isaria javanicus mutant strain IJUV-6 and dinotefuran comprises the following specific formula components: 60% Isaria javanicus mutant strain IJUV-6 spore powder (10)11Spore/g), 5% dinotefuran (a commercial raw drug), 0.8% nano-aluminum hydroxide, 4% tween-80, 5% sodium lignosulfonate, 3% suspending agent DK8 and diatomite to 100% to prepare 60% isaria javanicus mutant strain IJUV-6 and dinotefuran wettable powder; wherein the mass ratio of the Isaria javanicus mutant strain IJUV-6 spore powder to dinotefuran is 100: 8.33.
Example 850% oil solution of Isaria javanicus mutant strain IJUV-6 and dinotefuran
An oil solution of Isaria javanicus mutant strain IJUV-6 and dinotefuran comprises the following specific formula components: 50% Isaria javanicus mutant strain IJUV-6 spore powder (10)11Spore/g), 5% dinotefuran (a commercial original drug), 5% tween-80, 0.5% glycerol, 5% span-20, 1% white carbon black, 5% OP-10 and paraffin oil to 100% to prepare 50% of an oil solution of isaria javanica mutant IJUV-6 and dinotefuran; wherein the mass ratio of the Isaria javanicus mutant strain IJUV-6 to dinotefuran is 100: 10.
Comparative example 1 wettable powder lacking Isaria javanicus mutagenic strain IJUV-6 and dinotefuran
A wettable powder, except that there is no Isaria javanicus mutant strain IJUV-6 and dinotefuran, the other formulation ingredient content is the same as example 4; the specific formula comprises the following components in percentage by weight: 0.8 percent of nano aluminum hydroxide, 4 percent of tween-80, 5 percent of sodium lignosulfonate, 5 percent of wetting and suspending agent DK8 and diatomite which is supplemented to 100 percent to prepare the wettable powder.
Comparative example 2 wettable powder lacking dinotefuran
A wettable powder, except that no dinotefuran is added, the contents of the other formulation components are the same as those in example 5; the specific formula comprises the following components in percentage by weight: 50% Isaria javanicus mutant strain IJUV-6 spore powder (10)11Spore/g), 0.8 percent of nano aluminum hydroxide, 4 percent of tween-80, 5 percent of sodium lignosulfonate, 5 percent of wetting suspending agent DK8 and diatomite which is supplemented to 100 percent to prepare the wettable powder.
Comparative example 3 wettable powder lacking Isaria javanicus mutagenic strain IJUV-6
A wettable powder, except that there is no Isaria javanicus mutant strain IJUV-6, the other formulation component content is the same as example 4; the specific formula comprises the following components in percentage by weight: 8 percent of dinotefuran (a commercial raw drug), 0.8 percent of nano-aluminum hydroxide, 4 percent of tween-80, 5 percent of sodium lignosulfonate, 5 percent of Darun suspending agent DK8 and diatomite which are supplemented to 100 percent, thus preparing the wettable powder.
Comparative example 4
20% of dinotefuran, and the dosage is conventionally used in the field (the concentration is 133.3 mu g/mL in the field).
Application example 1 toxicity determination of compound insecticide on sogatella furcifera
Carrying out a toxicity determination experiment by using the compound pesticide of the Isaria javanicus mutant strain IJUV-6 and dinotefuran prepared in the embodiments 4-8 of the invention and the wettable powder prepared in the comparative examples 1-4, and determining the toxicity of the Isaria javanicus mutant strain to Sogatella furcifera; the specific experimental methods and experimental results are as follows:
1. experimental methods
(1) Test object
The test plant is rice (Oryza sativa L.), potted seedlings (the diameter of a pot is 30cm, the height of the seedlings is 40cm, 3 clusters/pot and 6-13 plants/cluster) are placed in an insect breeding cage, adult sogatella furcifera is inoculated to be completely expelled after spawning, and the sogatella furcifera at the stem part of the rice is grown to 4-year nymph for later use under the condition of 32-37 +/-1 ℃;
diluting 2000 times of the compound pesticide of the Isaria javanicus mutant strain IJUV-6 and dinotefuran prepared in the embodiments 4-8 and the wettable powder prepared in the comparative examples 1-3 to respectively obtain mixed liquor with different concentrations; preparing the dinotefuran in the comparative example 4 into liquid medicine with the concentration (133.3 mug/mL) which is conventionally used in the field, then respectively spraying the liquid medicine onto the leaves and stems of the rice in different treatment groups by adopting a spraying method, preferably, the rice is fully stained with liquid medicine droplets without dripping, treating 6 pots of seedlings for each time, investigating the effect 4 and 8 days after the pesticide application, repeating the whole experiment for 4 times, and calculating the corrected mortality rate of the sogatella furcifera; the corrected mortality rate of sogatella furcifera is calculated as follows:
corrected mortality (%) — treatment mortality-control mortality)/(1-control mortality) × 100.
2. Results of the experiment
The toxicity test results of the compound pesticide prepared by the invention to sogatella furcifera are shown in table 2, and it can be seen from the table that when the wettable powder does not contain isaria javanicus mutant strain IJUV-6 and dinotefuran, the control effect to sogatella furcifera is only 8.5% (4 days) and 11.8% (8 days); when the wettable powder does not contain dinotefuran, the control effect on sogatella furcifera is 40.4 percent (4 days) and 71.5 percent (8 days); when the wettable powder does not contain the Isaria javanicanicus mutant strain IJUV-6, the control effect on sogatella furcifera is 45.2 percent (4 days) and 47.5 percent (8 days); the control effect of the compound pesticide of the isaria javanicus mutant strain IJUV-6 and dinotefuran (example 4-8) on the sogatella furcifera is as high as 80.4-88% (4 days) and 89-96.1% (8 days), and is obviously higher than that of the wettable powder of comparative examples 1-3 on the sogatella furcifera; also significantly higher than the control effect of the conventional field recommended concentration of dinotefuran of comparative example 4.
The results show that the compound pesticide of the isaria javanica mutant strain IJUV-6 and dinotefuran prepared by the invention has obvious control effect on the sogatella furcifera, the isaria javanica mutant strain IJUV-6 and dinotefuran compound have synergistic effect on the control of the sogatella furcifera, the control effect of the compound pesticide on the sogatella furcifera is obviously higher than that of a single agent, the control effect is improved and the pesticidal time is shortened compared with a eubacterial pesticide, and the control effect is improved and the control duration time is prolonged compared with a chemical pesticide;
wherein the concentration of Isaria javanicus mutant strain IJUV-6 is 2.5x107spore/mL) compared with the prior art, the control effect of the compound agent on sogatella furcifera is improved by 99-118.6% (day 4) and 24.5-34.4% (day 8), which shows that the quick-acting property of the insecticide is improved after dinotefuran is added into the Isaria javanicus mutant strain IJUV-6, and compared with the chemical pesticide dinotefuran single agent (the using concentration of the dinotefuran is 133.3 mug/mL), the control effect of the compound agent on sogatella furcifera is improved by 77.9-118.6% (day 4) and 87.4-102.3% (day 8).
The above results show that: after the fungus insecticide is added into the chemical pesticide, the control effect is improved, and the control duration of the pesticide is prolonged; compared with the conventional dosage of dinotefuran and other chemical pesticides, the compound agent improves the control effect and reduces the dosage of the chemical pesticide dinotefuran.
Table 2 toxicity test results of the compounded insecticide prepared by the invention on Sogatella furcifera
Note: the same letters after the same numbers in the same column indicate no significant difference (DMRT method).
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (7)
1. The compound insecticide is characterized by comprising isaria javanica and dinotefuran;
the mass ratio of the Isaria javanicus to the dinotefuran is 100:8.33 to 25;
the Isaria javanicus is Isaria javanicus (Isaria javanica) mutant strain IJUV-6 which is preserved in China center for type culture collection 10 months and 10 days in 2019, and the preservation number is CCTCC NO: m2019800;
the Isaria javanicus mutant strain IJUV-6 is spore powder of Isaria javanicus mutant strain IJUV-6, and the spore content of the Isaria javanicus mutant strain IJUV-6 is 1011Spores/g.
2. The compound pesticide according to claim 1, wherein the mass ratio of the Isaria javanicus to the dinotefuran is 100:10 to 16.
3. The compound pesticide according to claim 1, wherein the mass ratio of the Isaria javanicus to the dinotefuran is 100: 16.
4. the application of the compound pesticide as claimed in any one of claims 1 to 3 in controlling rice planthoppers/rice leafhoppers or preparing biological agents for controlling the rice planthoppers/the rice leafhoppers.
5. The biological preparation for preventing and controlling rice planthoppers/rice leafhoppers is characterized by consisting of a main agent and an auxiliary agent; the main agent comprises Isaria javanicus mutagenic strain IJUV-6 and dinotefuran, and the auxiliary agent comprises a stabilizer, an auxiliary agent and a filler;
the mass ratio of the Isaria javanicus to the dinotefuran is 100:8.33 to 25;
the Isaria javanicus mutant strain IJUV-6 is preserved in the China center for type culture Collection in 2019, 10 months and 10 days, and the preservation number is CCTCC NO: m2019800;
the Isaria javanicus mutant strain IJUV-6 is spore powder of Isaria javanicus mutant strain IJUV-6, and the spore content of the Isaria javanicus mutant strain IJUV-6 is 1011Spores/g.
6. The biological agent according to claim 5, characterized in that it comprises, in mass percent: 50-65% of main agent, 0.5-1% of stabilizing agent, 12-20% of auxiliary agent and 22-35% of filler.
7. The biological preparation according to claim 5 or 6, wherein the biological preparation is in the form of wettable powder or oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN101755751A (en) * | 2009-12-29 | 2010-06-30 | 华南农业大学 | Pesticide by mixing tea saponin with insect growth regulator |
| KR20170053863A (en) * | 2015-11-07 | 2017-05-17 | 대한민국(농촌진흥청장) | Composition for simultaneous control of both aphid and Pythium ultimum using Isaria javanica Pf185 and its culture media |
| CN107897214A (en) * | 2017-12-26 | 2018-04-13 | 华南农业大学 | A kind of microorganism compounded pesticides for preventing rice grub and its application |
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| KR101330078B1 (en) * | 2011-12-06 | 2013-11-15 | 대한민국 | NEW ENTOMOPATHOGENIC FUNGI ISARIA JAVANICA Pf04 HAVING CONTROLLING OF BEMISIA TABACI Q TYPE AND MICROBIAL INSECTICIDE FOR CONTROLLING BEMISIA TABACI Q TYPE CONTAINING THE SAME |
| CN109479903B (en) * | 2018-11-28 | 2021-03-19 | 华南农业大学 | Isaria javanicus conidium oil suspending agent and application thereof |
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| CN101755751A (en) * | 2009-12-29 | 2010-06-30 | 华南农业大学 | Pesticide by mixing tea saponin with insect growth regulator |
| KR20170053863A (en) * | 2015-11-07 | 2017-05-17 | 대한민국(농촌진흥청장) | Composition for simultaneous control of both aphid and Pythium ultimum using Isaria javanica Pf185 and its culture media |
| CN107897214A (en) * | 2017-12-26 | 2018-04-13 | 华南农业大学 | A kind of microorganism compounded pesticides for preventing rice grub and its application |
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