CN110200019A - Kill the composition of planthopper - Google Patents
Kill the composition of planthopper Download PDFInfo
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- CN110200019A CN110200019A CN201910649118.XA CN201910649118A CN110200019A CN 110200019 A CN110200019 A CN 110200019A CN 201910649118 A CN201910649118 A CN 201910649118A CN 110200019 A CN110200019 A CN 110200019A
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- Prior art keywords
- trifluoro
- benzene pyrimidine
- preparation
- metarhizium anisopliae
- benzene
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The invention belongs to the biocide technical fields containing microbial fungi, and in particular to a kind of composition for killing planthopper.The composition includes Metarhizium anisopliae and trifluoro-benzene pyrimidine.Active constituent Metarhizium anisopliae and trifluoro-benzene pyrimidine in composition of the invention have the synergistic function to planthopper.
Description
Technical field
The invention belongs to the biocide technical fields containing microbial fungi, and in particular to a kind of combination for killing planthopper
Object.
Background technique
Planthopper belongs to Homoptera (Homoptera) Delphacidae (Delphacidae), is commonly called as midge worm, fiery midge worm, sound
Worm.Planthopper is current one of the primary pest for influencing rice in China stable and high yields.Planthopper is distributed widely in South Asia, the southeast
The rice producing region such as sub-, Pacific Islands and Japan, Korea, Australia, Egypt.In China, Hainan, Guangdong, Guangxi, Yunnan are removed
Southern winter has a small amount of worm sources survival outer, throughout the year cannot be overwintering in other most areas, and the initial worm sources of spring and autumn are mainly come
From in foreign countries.The adult and nymph of planthopper are clustered in rice clump base portion piercing and sucking juice, make by harm browning necrosis, nutrient and moisture
It scatters and disappears, causes yellow leaf, grow short, withered bar lodging when serious.Newborn advanced stage grouting is discontented, causes blighted grain, and mass of 1000 kernel is reduced,
Seriously affect yield (" in recent years China planthopper occur with study on prevention overview ", Xiao Haijun etc., chinese scientific papers is online,
Http:// www.paper.edu.cn/releasepaper/content/200701-334, the paragraph 1 1-4 row of page 1 and
2 sections of 1-3 rows, publication date on 01 24th, 2007).
Planthopper is migrating property of one kind, fulminant and destructive rice grub.The reproductive capacity of planthopper is fast, concealment
By force, the oncoming force is violent, can population outbreak in a short time, cause rice to reduce or total crop failure.In recent years, planthopper not only field occurrence quantity compared with
Greatly, it and is easy to cause stripe disease and black streaked dwarf virus of rice.In addition, with Rice Production system and tillage method
Continuous renewal, the cultivated area of China's single cropping rice increases year by year, and the high yield of rice relies primarily on dense planting with high fertile to realize,
This environment forms suitable temperature, is conducive to the generation of planthopper, these factors all further cause planthopper for
Aggravation (" screening of planthopper pesticide control and application technical research ", Li Hongyang etc., the Agriculture of Anhui section that rice safety production threatens
It learns, the 25th phase of volume 41 in 2013, the left column paragraph 1 1-6 row of page 10299, publication date on December 31st, 2013；" planthopper
The discussion with Preventing Countermeasures occurs ", Fang Hongping, the friend of farmer richness, the 22nd phase in 2013, the abstract 1-2 row of page 91 is open
On December 31st, 2013 day).
Therefore, the medicament that research can effectively kill planthopper is of great significance.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of composition for killing planthopper, activity in the composition at
Divide Metarhizium anisopliae and trifluoro-benzene pyrimidine that there is the synergistic function to planthopper.
To achieve the above object, the technical solution of the present invention is as follows:
The composition of planthopper is killed, active constituent includes Metarhizium anisopliae and trifluoro-benzene pyrimidine.
It is described to kill planthopper and refer to that substance improves the death rate of Homoptera Delphacidae insect or inhibits the ability of its growth rate.
Inventor has been surprisingly found that in the course of the research, the work in the composition including Metarhizium anisopliae and trifluoro-benzene pyrimidine
Property ingredient Metarhizium anisopliae and trifluoro-benzene pyrimidine have to the synergistic function of planthopper.
Further, the Metarhizium anisopliae is Metarhizium anisopliae CQMa421.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 1 × 200,000,000,000-10 ×
200000000000 spores: 1-10g trifluoro-benzene pyrimidine.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 4 × 200,000,000,000-8 ×
200000000000 spores: 2-8g trifluoro-benzene pyrimidine.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 6 × 200,000,000,000-7 ×
200000000000 spores: 4-6g trifluoro-benzene pyrimidine
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine is 7 × 200,000,000,000 spores
Son: 4g trifluoro-benzene pyrimidine.
The present invention also aims to protect the preparation for killing planthopper, active constituent includes Metarhizium anisopliae and trifluoro
Benzene pyrimidine.
Further, the Metarhizium anisopliae is Metarhizium anisopliae CQMa421.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 1 × 200,000,000,000-10 ×
200000000000 spores: 1-10g trifluoro-benzene pyrimidine.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 4 × 200,000,000,000-8 ×
200000000000 spores: 2-8g trifluoro-benzene pyrimidine.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine be 6 × 200,000,000,000-7 ×
200000000000 spores: 4-6g trifluoro-benzene pyrimidine.
Further, the proportion of Metarhizium anisopliae (specially xerospore powder) and trifluoro-benzene pyrimidine is 7 × 200,000,000,000 spores
Son: 4g trifluoro-benzene pyrimidine.
Further, the preparation further includes solvent and emulsifier.
Further, the solvent includes the vegetable oil such as rapeseed oil, sesame oil, corn oil or peanut oil.
Further, the emulsifier includes sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, rouge
Fat alcohol and ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine list olein, castor oil and ethylene oxide condensate
One or more of.
Further, the medicament further includes stabilizer.
Further, the stabilizer includes butylated hydroxyarisol, 2,6-di-tert-butyl p-cresol, gallic acid third
One of ester, tert-butylhydroquinone and ESCALOL 567 are a variety of.
Further, the dosage of the stabilizer is the 1%-5% of preparation gross mass.
Further, the dosage of the stabilizer is the 1%-3% of preparation gross mass.
The beneficial effects of the present invention are:
Active constituent Metarhizium anisopliae and trifluoro-benzene pyrimidine in composition of the invention have the collaboration to planthopper
Synergistic effect.
Composition efficacy stability of the invention.
Composition quick-acting of the invention is good.
Composition pest of the invention is not likely to produce drug resistance.
Specific embodiment
Illustrated embodiment is to preferably be illustrated to the contents of the present invention, but is not that the contents of the present invention only limit
In illustrated embodiment.So those skilled in the art carry out nonessential change to embodiment according to foregoing invention content
Into and adjustment, still fall within protection scope of the present invention.Unless otherwise specified, technological means used in embodiment is this field skill
Conventional means known to art personnel.
Indoor biometrics compound joint toxicity measuring test:
It is (only green by chafer to detect Metarhizium anisopliae CQMa421 xerospore powder, trifluoro-benzene pyrimidine single dose and combinations thereof
Stiff bacterium CQMa421 xerospore powder and trifluoro-benzene pyrimidine composition, are specifically shown in Table the virulence 1) to black peach aphid, meanwhile, co-toxicity coefficient is calculated,
Test result is as shown in table 1.
Reagent agent: Metarhizium anisopliae CQMa421 xerospore powder, 96%wt trifluoro-benzene pyrimidine raw medicine.
Selected insect source: planthopper is adopted in University Of Chongqing's base of plant paddy field in July, 2015, indoors using without medicine
Rice seedlings raising, 26 ± 1 DEG C of raising temperature, relative humidity 75% ± 5%, light application time L:D=16:8h.
Test method:
Insecticidal toxicity measuring method: rice ware worm Potter spray-on process is used.6 plants of rice seedling are taken, diameter 6.5cm culture dish is placed in
In, every ware adds water 10ml, is fixed on ware center with drystone sand, strikes off along ware face.Nymph, CO are drawn with pest sucking device2Anesthesia, often
Ware filters out 3 age mid-term nymph 30 of the same size and is connected on rice seedling, in Potter spray tower (pressure 34.5kPa, settling amount
For 4.35mg/cm2) metered dose；Every ware spray value 2.5ml, 3 repetitions of every processing；
72h investigates the death rate of test worm after medicine, and carries out data processing, compares the activity of reagent agent.
Data processing:
Virulence regression equation is calculated using POLO Plus software.Calculating parameter include: slope b value and standard error, card square,
Freedom degree, LC50Value and 95% confidence limit.
Co-toxicity evaluation method:
Using Sun Yunpei co-toxicity coefficient method:
Toxicity index (TI)=(LC of standard agent50The LC of/reagent agent50)×100
The LC that mixture actual toxicity index (ATI)=medicament A is applied alone50LC/medicament A mixed with B50)×100
Mixtures theoretical toxicity index (TTI)=TI (A) × a+TI (B) × b
A: percentage composition of the medicament A in mixture is indicated；
B: percentage composition of the medicament B in mixture is indicated.
Co-toxicity coefficient CTC=(mixture actual toxicity index ATI/ mixtures theoretical toxicity index TTI) × 100；
According to the regulations of the Ministry of Agriculture ' s Pesticide Testing Institute, the judgment criteria of mixture formula synergy are as follows: co-toxicity coefficient is greater than 120 and is
Synergistic effect；Co-toxicity coefficient 80-120 is summation action；Co-toxicity coefficient is antagonism less than 80；
For above each group in addition to medicament is different, other processing modes are identical.
The results are shown in Table 1.
The virulence test result of 1 green muscardine fungus of table and trifluoro-benzene pyrimidine single dose and combinations thereof to planthopper
| Medicament and proportion | LC50(mg/L) | Co-toxicity coefficient CTC |
| A: green muscardine fungus | 62.08 | - |
| B: trifluoro-benzene pyrimidine | 3.69 | - |
| A:B=10:1 | 12.41 | 205.2 |
| A:B=9:2 | 7.06 | 226.7 |
| A:B=8:3 | 4.88 | 239.5 |
| A:B=7:4 | 2.80 | 328.7 |
| A:B=6:5 | 2.83 | 268.2 |
| A:B=5:6 | 2.78 | 232.1 |
| A:B=4:7 | 2.81 | 199.3 |
| A:B=3:8 | 2.41 | 205.8 |
| A:B=2:9 | 2.02 | 220.1 |
| A:B=1:10 | 2.01 | 200.9 |
Remarks: green muscardine fungus CQMa421 spore content used be 20,500,000,000 spores/gram, in table proportion for simplify description, green muscardine fungus:
Trifluoro-benzene pyrimidine=x:y refers to that muscardine spore number is x × 200,000,000,000: y/ grams of weight of trifluoro-benzene pyrimidine.
As shown in Table 1, in the grams ratio of Metarhizium anisopliae CQMa421 xerospore powder miospore number and trifluoro-benzene pyrimidine
In (1-10) × 200,000,000,000 spore: within the scope of 1-10g trifluoro-benzene pyrimidine, the two compounding, co-toxicity coefficient is all larger than 120,
Show synergistic function；Wherein in 7 × 200,000,000,000 spores: when 4g trifluoro-benzene pyrimidine, synergistic function is the most significant.
Thus it proves, Metarhizium anisopliae CQMa421 is compounded with trifluoro-benzene pyrimidine with synergistic function, can effectively be killed rice and be flown
Lice.
Embodiment 1
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 20,000,000,000/gram, trifluoro-benzene pyrimidine content is 1%wt, and muscardine spore number: trifluoro-benzene pyrimidine weight is 10 × 200,000,000,000
Spore: 1g trifluoro-benzene pyrimidine, addition 6%wt sorbitan fatty acid ester polyoxyethylene ether, 3%wt tert-butylhydroquinone,
1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, quality of the percentage to account for preparation gross mass
Percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 2
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 18,000,000,000/gram, trifluoro-benzene pyrimidine content is 2%wt, and muscardine spore number: trifluoro-benzene pyrimidine weight is 9 × 200,000,000,000 spores
Son: 2g trifluoro-benzene pyrimidine, addition 6%wt sorbitan fatty acid ester polyoxyethylene ether, 3%wt tert-butylhydroquinone, 1%
Wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, quality percentage of the percentage to account for preparation gross mass
Than meter.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 3
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 16,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 3%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 8 × 200,000,000,000
A spore: 3g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 4
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 14,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 4%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 7 × 200,000,000,000
A spore: 4g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 5
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 12,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 5%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 6 × 200,000,000,000
A spore: 5g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 6
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 10,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 6%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 5 × 200,000,000,000
A spore: 6g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 7
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 8,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 7%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 4 × 200,000,000,000
A spore: 7g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 8
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 6,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 8%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 3 × 200,000,000,000
A spore: 8g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 9
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 4,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 9%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 2 × 200,000,000,000
A spore: 9g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 10
Preparation, active constituent are Metarhizium anisopliae CQMa421 and trifluoro-benzene pyrimidine, wherein green muscardine fungus xerospore powder content
For 2,000,000,000 spores/gram, trifluoro-benzene pyrimidine content be 10%wt, muscardine spore number: trifluoro-benzene pyrimidine weight be 1 × 200,000,000,000
A spore: 10g trifluoro-benzene pyrimidine adds 6%wt sorbitan fatty acid ester polyoxyethylene ether, and 3%wt tertiary butyl is to benzene two
Phenol, 1%wt bentonite, 0.8%wt white carbon black supply 100% with salad oil, matter of the percentage to account for preparation gross mass
Measure percentages.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Embodiment 11
Preparation, active constituent are Metarhizium anisopliae CQMa421, and wherein green muscardine fungus xerospore powder content is 10,000,000,000
Spore/gram, add 6%wt sorbitan fatty acid ester polyoxyethylene ether, 3%wt tert-butylhydroquinone, 1%wt swelling
Soil, 0.8%wt white carbon black supply 100% with salad oil, and the percentage is in terms of the mass percent for accounting for preparation gross mass.
The preparation the preparation method comprises the following steps:
All the components in addition to muscardine spore are mixed, are uniformly mixed through high speed shear, then 2-3 is sanded by sand mill
Hour, so that average grain diameter is reached 1-5 microns, green muscardine fungus xerospore powder is added, obtains preparation after mixing.
Indoor compatibility test
The formulation samples of embodiment 1-11 are placed in 37 DEG C of incubators and are incubated for, detect spore germination respectively at 3d, 14d
Rate, determines whether trifluoro-benzene pyrimidine has an impact to the sprouting of muscardine spore.Spore germination rate detection method: utilize sterile water will
It is 1 × 10 that each formulation samples, which are diluted to spore content,7/ ml takes on 50uL spore suspension even spread to 1/4SDAY plate, 28 degree
Germination rate is detected after culture 14h；
Spore germination rate detection method: it is 1 × 10 that each formulation samples, which are diluted to spore content, using sterile water7/ ml, takes
On 50uL spore suspension even spread to 1/4SDAY plate, germination rate is detected after 28 degree of culture 14h；The above each group is different except medicament
Outside, other way to manages are identical.
Germination rate result of the 2 embodiment 1-11 of table after 37 DEG C of incubations 3d, 14d
| Sample | 37 DEG C of germination rates after 3 days | 37 DEG C of germination rates after 14 days |
| Embodiment 1 | 85.2%Aa | 65.2%Aa |
| Embodiment 2 | 89.4%Aa | 64.9%Aa |
| Embodiment 3 | 83.6%Aa | 66.2%Aa |
| Embodiment 4 | 84.7%Aa | 64.8%Aa |
| Embodiment 5 | 86.9%Aa | 62.8%Aa |
| Embodiment 6 | 84.2%Aa | 64.8%Aa |
| Embodiment 7 | 86.9%Aa | 67.1%Aa |
| Embodiment 8 | 84.7%Aa | 65.8%Aa |
| Embodiment 9 | 86.4%Aa | 63.3%Aa |
| Embodiment 10 | 89.2%Aa | 64.8%Aa |
| Embodiment 11 | 86.4%Aa | 64.5%Aa |
Remarks: lowercase difference indicates that, in 0.05 horizontal upper significant difference, capitalization difference is indicated in 0.01 level
Upper significant difference.
As shown in Table 2, there was no significant difference for the germination rate of the preparation of embodiment 1-11.Thus prove, trifluoro-benzene pyrimidine with
The good compatibility of Metarhizium anisopliae CQMa421.
Field information test: field rice brown paddy plant hopper test
Water is done with the preparation and 10%wt trifluoro-benzene pyrimidine suspending agent (being purchased from Dow E.I.Du Pont Company) of embodiment 1-11
Brown plant-hopper field control comparative test, while blank control group is set；
Specifically: in beginning peak period medication, medication 1 time, before medicine, 3 days after medicine, 7 days, 21 days investigation borer populations and calculate prevention and treatment
Effect, control efficiency calculate as follows:
In formula, before PT0 indicates application, after PT1 indicates application, PT indicates chemicals treatment district, and CK indicates blank control group；
For above each group in addition to medicament is different, other processing modes are identical；
The results are shown in Table 3.
3 green muscardine fungus of table compounds prevention and treatment Brown Planthopper field test results with trifluoro-benzene pyrimidine
Note: lowercase difference indicates that, in 0.05 horizontal upper significant difference, capitalization difference indicates in 0.01 level
Significant difference；It is consistent that the application dosage respectively handled is converted into production cost, is disposed as 8 yuan/mu.
As shown in Table 3, under identical cost, synergy is obvious after mixture according to the above ratio, bright to the preventive effect of rice hoppers
It is aobvious to be better than single dose.Thus it proves, medicament of the invention is excellent to the control efficiency of planthopper, and quick-acting is good, and the lasting period is long.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (10)
1. killing the composition of planthopper, which is characterized in that active constituent includes Metarhizium anisopliae and trifluoro-benzene pyrimidine.
2. composition according to claim 1, which is characterized in that the Metarhizium anisopliae is Metarhizium anisopliae
CQMa421。
3. composition according to claim 1 or 2, which is characterized in that the proportion of Metarhizium anisopliae and trifluoro-benzene pyrimidine
For 1 × 200,000,000,000-10 × 200,000,000,000 spores: 1-10g trifluoro-benzene pyrimidine.
4. composition according to claim 3, which is characterized in that the proportion of Metarhizium anisopliae and trifluoro-benzene pyrimidine is 4
× 2,000 hundred million -8 × 200,000,000,000 spores: 2-8g trifluoro-benzene pyrimidine.
5. composition according to claim 4, which is characterized in that the proportion of Metarhizium anisopliae and trifluoro-benzene pyrimidine is 6
× 2,000 hundred million -7 × 200,000,000,000 spores: 4-6g trifluoro-benzene pyrimidine.
6. composition according to claim 5, which is characterized in that the proportion of Metarhizium anisopliae and trifluoro-benzene pyrimidine is 7
× 2,000 hundred million spores: 4g trifluoro-benzene pyrimidine.
7. killing the preparation of planthopper, which is characterized in that active constituent includes Metarhizium anisopliae and trifluoro-benzene pyrimidine.
8. preparation according to claim 7, which is characterized in that the preparation further includes solvent and emulsifier.
9. preparation according to claim 8, which is characterized in that the preparation further includes stabilizer.
10. preparation according to claim 8 or claim 9, which is characterized in that the stabilizer includes tert-butyl to hydroxyl fennel
In ether, 2,6-di-tert-butyl p-cresol, propylgallate, tert-butylhydroquinone and ESCALOL 567
It is one kind of multiple.
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| CN201910649118.XA CN110200019A (en) | 2019-07-18 | 2019-07-18 | Kill the composition of planthopper |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110495476A (en) * | 2019-09-24 | 2019-11-26 | 重庆谷百奥生物研究院有限公司 | Metarhizium anisopliae and two diuril pyrimidines compounding kill the composition of striped rice borer |
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| CN107455379A (en) * | 2017-09-22 | 2017-12-12 | 陕西嘉益蓝德生物工程有限公司 | A kind of ultra low volume liquids of pyrimidine containing trifluoro-benzene and its application |
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